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PLA2G2D cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PLA2G2D
Entrez Gene ID 26279
Full Name phospholipase A2 group IID
Synonyms PLA2IID,SPLASH,sPLA2-IID,sPLA2S
General protein information
Preferred Names

phospholipase A2 group IID

Names

group IID secretory phospholipase A2
GIID sPLA2
phosphatidylcholine 2-acylhydrolase 2D
secretory phospholipase A2s
secretory-type PLA, stroma-associated homolog

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

1

1p36.12

Summary This gene encodes a secreted member of the phospholipase A2 family, and is found in a cluster of related family members on chromosome 1. Phospholipase A2 family members hydrolyze the sn-2 fatty acid ester bond of glycerophospholipids to produce lysophospholipids and free fatty acid. This gene may be involved in inflammation and immune response, and in weight loss associated with chronic obstructive pulmonary disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012].
Disorder MIM:

605630


mRNA and Protein(s)

mRNA Protein Name
NM_012400.3 NP_036532.1 group IID secretory phospholipase A2 isoform 1 precursor
NM_001271814.1 NP_001258743.1 group IID secretory phospholipase A2 isoform 2 precursor



Homo sapiens (human) PLA2G2D NP_036532.1
Macaca mulatta (rhesus monkey) PLA2G2D XP_001095528.1
Pan troglodytes (chimpanzee) PLA2G2D XP_524585.3
Rattus norvegicus (Norway rat) Pla2g2d NP_001013446.1
Canis lupus familiaris (dog) PLA2G2D XP_544524.3
Mus musculus (house mouse) Pla2g2d NP_035239.1


Related articles in PubMed

Critical role of phospholipase A2 group IID in age-related susceptibility to severe acute respiratory syndrome-CoV infection.
Vijay R, Hua X, Meyerholz DK, Miki Y, Yamamoto K, Gelb M, Murakami M, Perlman S
The Journal of experimental medicine212(11)1851-68(2015 Oct)

Assessing phospholipase A2 activity toward cardiolipin by mass spectrometry.
Hsu YH, Dumlao DS, Cao J, Dennis EA
PloS one8(3)e59267(2013)

Secretory phospholipase A2 group IID Is involved in progesterone-induced acrosomal exocytosis of human spermatozoa.
Li K, Jin JY, Chen WY, Shi QX, Ni Y, Roldan ER
Journal of andrology33(5)975-83(2012 Sep-Oct)

The bactericidal effect of human secreted group IID phospholipase A2 results from both hydrolytic and non-hydrolytic activities.
Fonseca-Maldonado R, Ferreira TL, Ward RJ
Biochimie94(6)1437-40(2012 Jun)

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA, Butte NF
PloS one7(12)e51954(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

found to induce PLA2G2D expression in mice and in human monocyte-derived macrophages
Title: Critical role of phospholipase A2 group IID in age-related susceptibility to severe acute respiratory syndrome-CoV infection.

sPLA(2)-IID is present at the head and midpiece in the human sperm, and its activation seems to be involved in acrosomal exocytosis.
Title: Secretory phospholipase A2 group IID Is involved in progesterone-induced acrosomal exocytosis of human spermatozoa.

phospholipid bilayer permeabilization by the hsPLA2GIID is independent of catalytic activity.
Title: The bactericidal effect of human secreted group IID phospholipase A2 results from both hydrolytic and non-hydrolytic activities.

Observational study of gene-disease association. (HuGE Navigator)
Title: New genetic associations detected in a host response study to hepatitis B vaccine.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Polymorphisms in innate immunity genes and patients response to dendritic cell-based HIV immuno-treatment.

The following PLA2G2D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLA2G2D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu09864 NM_001271814.1
Latest version!
Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu10091 NM_012400.3
Latest version!
Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu09864
Clone ID Related Accession (Same CDS sequence) NM_001271814.1
Accession Version NM_001271814.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 189bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-08-26
Organism Homo sapiens(Human)
Product group IID secretory phospholipase A2 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC295429.1, AK310156.1, BC025706.1 and AK026347.1. Transcript Variant: This variant (2) lacks an internal exon in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK310156.1, DA982802.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:10455175) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG
GACTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001258743.1
CDS69..257
Misc Feature(1)129..>254
Exon (1)1..108
Exon (2)109..253
Exon (3)254..1863
Translation

Target ORF information:

RefSeq Version NM_001271814.1
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001271814.1

ORF Insert Sequence:

1
61
121
181
ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG
GACTGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10091
Clone ID Related Accession (Same CDS sequence) NM_012400.3
Accession Version NM_012400.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 438bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-08-26
Organism Homo sapiens(Human)
Product group IID secretory phospholipase A2 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC295429.1, BC025706.1 and AK026347.1. On Nov 28, 2012 this sequence version replaced NM_012400.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF188625.1, AK290406.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2155628 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:10455175) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG
GACTGGTGCT GCCAGACCCA TGACTGCTGC TATGACCACC TGAAGACCCA GGGGTGCAGC
ATCTACAAGG ACTATTACAG ATACAACTTT TCCCAGGGGA ACATCCACTG CTCTGACAAG
GGAAGCTGGT GTGAGCAGCA GCTGTGTGCC TGTGACAAGG AGGTGGCCTT CTGCCTGAAG
CGCAACCTGG ACACCTACCA GAAGCGACTG CGTTTCTACT GGCGGCCCCA CTGCCGGGGG
CAGACCCCTG GGTGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_036532.1
CDS69..506
Misc Feature(1)129..482
Misc Feature(2)order(132..134,141..143,153..155,183..185,216..218,312..314)
Misc Feature(3)order(207..209,213..215,219..221,270..272)
Misc Feature(4)order(213..215,267..272,279..281,324..326,402..404)
Exon (1)1..108
Exon (2)109..253
Exon (3)254..360
Exon (4)361..1970
Translation

Target ORF information:

RefSeq Version NM_012400.3
Organism Homo sapiens(Human)
Definition Homo sapiens phospholipase A2 group IID (PLA2G2D), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_012400.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGAACTTG CACTGCTGTG TGGGCTGGTG GTGATGGCTG GTGTGATTCC AATCCAGGGC 
GGGATCCTGA ACCTGAACAA GATGGTCAAG CAAGTGACTG GGAAAATGCC CATCCTCTCC
TACTGGCCCT ACGGCTGTCA CTGCGGACTA GGTGGCAGAG GCCAACCCAA AGATGCCACG
GACTGGTGCT GCCAGACCCA TGACTGCTGC TATGACCACC TGAAGACCCA GGGGTGCAGC
ATCTACAAGG ACTATTACAG ATACAACTTT TCCCAGGGGA ACATCCACTG CTCTGACAAG
GGAAGCTGGT GTGAGCAGCA GCTGTGTGCC TGTGACAAGG AGGTGGCCTT CTGCCTGAAG
CGCAACCTGG ACACCTACCA GAAGCGACTG CGTTTCTACT GGCGGCCCCA CTGCCGGGGG
CAGACCCCTG GGTGCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Critical role of phospholipase A2 group IID in age-related susceptibility to severe acute respiratory syndrome-CoV infection.
The Journal of experimental medicine212(11)1851-68(2015 Oct)
Vijay R,Hua X,Meyerholz DK,Miki Y,Yamamoto K,Gelb M,Murakami M,Perlman S


book

Assessing phospholipase A2 activity toward cardiolipin by mass spectrometry.
PloS one8(3)e59267(2013)
Hsu YH,Dumlao DS,Cao J,Dennis EA


book

Secretory phospholipase A2 group IID Is involved in progesterone-induced acrosomal exocytosis of human spermatozoa.
Journal of andrology33(5)975-83(2012 Sep-Oct)
Li K,Jin JY,Chen WY,Shi QX,Ni Y,Roldan ER


book

The bactericidal effect of human secreted group IID phospholipase A2 results from both hydrolytic and non-hydrolytic activities.
Biochimie94(6)1437-40(2012 Jun)
Fonseca-Maldonado R,Ferreira TL,Ward RJ


book

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
PloS one7(12)e51954(2012)
Comuzzie AG,Cole SA,Laston SL,Voruganti VS,Haack K,Gibbs RA,Butte NF


 
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