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AMELX cDNA ORF clone, Homo sapiens(Human)

Gene Symbol AMELX
Entrez Gene ID 265
Full Name amelogenin, X-linked
Synonyms AI1E,AIH1,ALGN,AMG,AMGL,AMGX
General protein information
Preferred Names

amelogenin, X-linked

Names

amelogenin, X isoform
amelogenin (X chromosome, amelogenesis imperfecta 1)
amelogenin (amelogenesis imperfecta 1, X-linked)

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

X

Xp22.2

Summary This gene encodes a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in this gene cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008].
Disorder MIM:

300391

Disorder Html: Amelogenesis imperfecta, hypoplastic/hypomaturation type, 301200 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_017029404.1 XP_016884893.1 amelogenin, X isoform isoform X1
NM_182680.1 NP_872621.1 amelogenin, X isoform isoform 3
XM_011545489.1 XP_011543791.1 amelogenin, X isoform isoform X1
NM_001142.2 NP_001133.1 amelogenin, X isoform isoform 1 precursor
NM_182681.1 NP_872622.1 amelogenin, X isoform isoform 2



Homo sapiens (human) AMELX NP_872621.1
Canis lupus familiaris (dog) AMELX XP_003435493.1
Rattus norvegicus (Norway rat) Amelx NP_062027.1
Bos taurus (cattle) AMELX NP_001014984.1
Macaca mulatta (rhesus monkey) AMELX NP_001091983.1


Related articles in PubMed

Full-length amelogenin influences the differentiation of human dental pulp stem cells.
Frasheri I, Ern C, Diegritz C, Hickel R, Hristov M, Folwaczny M
Stem cell research & therapy710(2016 Jan)

Association of Amelogenin with High Caries Experience in Indian Children.
Saha R, Sood PB, Sandhu M, Diwaker A, Upadhyaye S
The Journal of clinical pediatric dentistry39(5)458-61(2015)

Amelogenin in Enamel Tissue Engineering.
Uskoković V
Advances in experimental medicine and biology881237-54(2015)

Enamel formation genes associated with dental erosive wear.
Søvik JB, Vieira AR, Tveit AB, Mulic A
Caries research49(3)236-42(2015)

A pituitary homeobox 2 (Pitx2):microRNA-200a-3p:β-catenin pathway converts mesenchymal cells to amelogenin-expressing dental epithelial cells.
Sharp T, Wang J, Li X, Cao H, Gao S, Moreno M, Amendt BA
The Journal of biological chemistry289(39)27327-41(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a single-nucleotide polymorphism in the amelogenin gene using amplified product-length polymorphisms in combination with sex-determining region Y analysis, is reported.
Title: Sex Determination from Fragmented and Degenerated DNA by Amplified Product-Length Polymorphism Bidirectional SNP Analysis of Amelogenin and SRY Genes.

Full-length amelogenin may have a negative mitogenic impact on human dental pulp stem cells.
Title: Full-length amelogenin influences the differentiation of human dental pulp stem cells.

sequencing data showed presence of mutation. Samples showing mutation (43.3%) showed high correlation with caries (80.7%) experience which was statistically significant.
Title: Association of Amelogenin with High Caries Experience in Indian Children.

Studies indicate that a single point mutation (41Pro-->Thr) in the amelogenin gene causes severe dental enamel malformation known as amelogenesis imperfect.
Title: Amelogenin in Enamel Tissue Engineering.

suggestive overrepresentation of TT genotype of amelogenin marker in cases w/severe erosion when compared to no dentine erosion. Amelogenin also associated with severe erosion in recessive model; TT genotype significantly more frequent in affected group
Title: Enamel formation genes associated with dental erosive wear.

The following AMELX gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AMELX cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25363
NM_182681.1
Latest version!
Homo sapiens amelogenin, X-linked (AMELX), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu25376
NM_182680.1
Latest version!
Homo sapiens amelogenin, X-linked (AMELX), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu25376
XM_011545489.1
Latest version!
Homo sapiens amelogenin, X-linked (AMELX), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu25531 NM_001142.2
Latest version!
Homo sapiens amelogenin, X-linked (AMELX), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu99236 XM_017029404.1
Latest version!
Homo sapiens amelogenin, X-linked (AMELX), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00

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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25363
Clone ID Related Accession (Same CDS sequence) NM_182681.1
Accession Version NM_182681.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 528bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-16
Organism Homo sapiens(Human)
Product amelogenin, X isoform isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002366.1. Transcript Variant: This variant (2) lacks two in-frame exons in the coding region, compared to variant 3. Isoform 2 is shorter than isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
ATGGGGACCT GGATTTTATT TGCCTGCCTC CTGGGAGCAG CTTTTGCCAT GCCTGTGCTT 
ACCCCTTTGA AGTGGTACCA GAGCATAAGG CCACCGTACC CTTCCTATGG TTACGAGCCC
ATGGGTGGAT GGCTGCACCA CCAAATCATC CCCGTGCTGT CCCAACAGCA CCCCCCGACT
CACACCCTGC AGCCTCATCA CCACATCCCA GTGGTGCCAG CTCAGCAGCC CGTGATCCCC
CAGCAACCAA TGATGCCCGT TCCTGGCCAA CACTCCATGA CTCCAATCCA ACACCACCAG
CCAAACCTCC CTCCGCCCGC CCAGCAGCCC TACCAGCCCC AGCCTGTTCA GCCACAGCCT
CACCAGCCCA TGCAGCCCCA GCCACCTGTG CACCCCATGC AGCCCCTGCC GCCACAGCCA
CCTCTGCCTC CGATGTTCCC CATGCAGCCC CTGCCTCCCA TGCTTCCTGA TCTGACTCTG
GAAGCTTGGC CATCAACAGA CAAGACCAAG CGGGAGGAAG TGGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_872622.1
CDS69..596
Misc Feature(1)42..44
Misc Feature(2)111..593
Exon (1)1..56
Exon (2)57..122
Exon (3)123..164
Exon (4)165..590
Exon (5)591..745
Translation

Target ORF information:

RefSeq Version NM_182681.1
Organism Homo sapiens(Human)
Definition Homo sapiens amelogenin, X-linked (AMELX), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_182681.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGGGACCT GGATTTTATT TGCCTGCCTC CTGGGAGCAG CTTTTGCCAT GCCTGTGCTT 
ACCCCTTTGA AGTGGTACCA GAGCATAAGG CCACCGTACC CTTCCTATGG TTACGAGCCC
ATGGGTGGAT GGCTGCACCA CCAAATCATC CCCGTGCTGT CCCAACAGCA CCCCCCGACT
CACACCCTGC AGCCTCATCA CCACATCCCA GTGGTGCCAG CTCAGCAGCC CGTGATCCCC
CAGCAACCAA TGATGCCCGT TCCTGGCCAA CACTCCATGA CTCCAATCCA ACACCACCAG
CCAAACCTCC CTCCGCCCGC CCAGCAGCCC TACCAGCCCC AGCCTGTTCA GCCACAGCCT
CACCAGCCCA TGCAGCCCCA GCCACCTGTG CACCCCATGC AGCCCCTGCC GCCACAGCCA
CCTCTGCCTC CGATGTTCCC CATGCAGCCC CTGCCTCCCA TGCTTCCTGA TCTGACTCTG
GAAGCTTGGC CATCAACAGA CAAGACCAAG CGGGAGGAAG TGGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25376
Clone ID Related Accession (Same CDS sequence) NM_182680.1 , XM_011545489.1
Accession Version NM_182680.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-16
Organism Homo sapiens(Human)
Product amelogenin, X isoform isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF436849.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) encodes the longest isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
ATGGGGACCT GGATTTTATT TGCCTGCCTC CTGGGAGCAG CTTTTGCCAT GCCTCTACCA 
CCTCATCCTG GGCACCCTGG TTATATCAAC TTCAGCTATG AGAACTCACA TTCTCAGGCT
ATCAATGTTG ACAGGACTGC ATTAGTGCTT ACCCCTTTGA AGTGGTACCA GAGCATAAGG
CCACCGTACC CTTCCTATGG TTACGAGCCC ATGGGTGGAT GGCTGCACCA CCAAATCATC
CCCGTGCTGT CCCAACAGCA CCCCCCGACT CACACCCTGC AGCCTCATCA CCACATCCCA
GTGGTGCCAG CTCAGCAGCC CGTGATCCCC CAGCAACCAA TGATGCCCGT TCCTGGCCAA
CACTCCATGA CTCCAATCCA ACACCACCAG CCAAACCTCC CTCCGCCCGC CCAGCAGCCC
TACCAGCCCC AGCCTGTTCA GCCACAGCCT CACCAGCCCA TGCAGCCCCA GCCACCTGTG
CACCCCATGC AGCCCCTGCC GCCACAGCCA CCTCTGCCTC CGATGTTCCC CATGCAGCCC
CTGCCTCCCA TGCTTCCTGA TCTGACTCTG GAAGCTTGGC CATCAACAGA CAAGACCAAG
CGGGAGGAAG TGGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_872621.1
CDS69..686
Misc Feature(1)42..44
Misc Feature(2)150..683
Exon (1)1..56
Exon (2)57..122
Exon (3)123..170
Exon (4)171..212
Exon (5)213..254
Exon (6)255..680
Exon (7)681..835
Translation

Target ORF information:

RefSeq Version NM_182680.1
Organism Homo sapiens(Human)
Definition Homo sapiens amelogenin, X-linked (AMELX), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_182680.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGGGACCT GGATTTTATT TGCCTGCCTC CTGGGAGCAG CTTTTGCCAT GCCTCTACCA 
CCTCATCCTG GGCACCCTGG TTATATCAAC TTCAGCTATG AGAACTCACA TTCTCAGGCT
ATCAATGTTG ACAGGACTGC ATTAGTGCTT ACCCCTTTGA AGTGGTACCA GAGCATAAGG
CCACCGTACC CTTCCTATGG TTACGAGCCC ATGGGTGGAT GGCTGCACCA CCAAATCATC
CCCGTGCTGT CCCAACAGCA CCCCCCGACT CACACCCTGC AGCCTCATCA CCACATCCCA
GTGGTGCCAG CTCAGCAGCC CGTGATCCCC CAGCAACCAA TGATGCCCGT TCCTGGCCAA
CACTCCATGA CTCCAATCCA ACACCACCAG CCAAACCTCC CTCCGCCCGC CCAGCAGCCC
TACCAGCCCC AGCCTGTTCA GCCACAGCCT CACCAGCCCA TGCAGCCCCA GCCACCTGTG
CACCCCATGC AGCCCCTGCC GCCACAGCCA CCTCTGCCTC CGATGTTCCC CATGCAGCCC
CTGCCTCCCA TGCTTCCTGA TCTGACTCTG GAAGCTTGGC CATCAACAGA CAAGACCAAG
CGGGAGGAAG TGGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25376
Clone ID Related Accession (Same CDS sequence) NM_182680.1 , XM_011545489.1
Accession Version XM_011545489.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product amelogenin, X isoform isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
ATGGGGACCT GGATTTTATT TGCCTGCCTC CTGGGAGCAG CTTTTGCCAT GCCTCTACCA 
CCTCATCCTG GGCACCCTGG TTATATCAAC TTCAGCTATG AGAACTCACA TTCTCAGGCT
ATCAATGTTG ACAGGACTGC ATTAGTGCTT ACCCCTTTGA AGTGGTACCA GAGCATAAGG
CCACCGTACC CTTCCTATGG TTACGAGCCC ATGGGTGGAT GGCTGCACCA CCAAATCATC
CCCGTGCTGT CCCAACAGCA CCCCCCGACT CACACCCTGC AGCCTCATCA CCACATCCCA
GTGGTGCCAG CTCAGCAGCC CGTGATCCCC CAGCAACCAA TGATGCCCGT TCCTGGCCAA
CACTCCATGA CTCCAATCCA ACACCACCAG CCAAACCTCC CTCCGCCCGC CCAGCAGCCC
TACCAGCCCC AGCCTGTTCA GCCACAGCCT CACCAGCCCA TGCAGCCCCA GCCACCTGTG
CACCCCATGC AGCCCCTGCC GCCACAGCCA CCTCTGCCTC CGATGTTCCC CATGCAGCCC
CTGCCTCCCA TGCTTCCTGA TCTGACTCTG GAAGCTTGGC CATCAACAGA CAAGACCAAG
CGGGAGGAAG TGGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543791.1
Translation

Target ORF information:

RefSeq Version XM_011545489.1
Organism Homo sapiens(Human)
Definition Homo sapiens amelogenin, X-linked (AMELX), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011545489.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGGGACCT GGATTTTATT TGCCTGCCTC CTGGGAGCAG CTTTTGCCAT GCCTCTACCA 
CCTCATCCTG GGCACCCTGG TTATATCAAC TTCAGCTATG AGAACTCACA TTCTCAGGCT
ATCAATGTTG ACAGGACTGC ATTAGTGCTT ACCCCTTTGA AGTGGTACCA GAGCATAAGG
CCACCGTACC CTTCCTATGG TTACGAGCCC ATGGGTGGAT GGCTGCACCA CCAAATCATC
CCCGTGCTGT CCCAACAGCA CCCCCCGACT CACACCCTGC AGCCTCATCA CCACATCCCA
GTGGTGCCAG CTCAGCAGCC CGTGATCCCC CAGCAACCAA TGATGCCCGT TCCTGGCCAA
CACTCCATGA CTCCAATCCA ACACCACCAG CCAAACCTCC CTCCGCCCGC CCAGCAGCCC
TACCAGCCCC AGCCTGTTCA GCCACAGCCT CACCAGCCCA TGCAGCCCCA GCCACCTGTG
CACCCCATGC AGCCCCTGCC GCCACAGCCA CCTCTGCCTC CGATGTTCCC CATGCAGCCC
CTGCCTCCCA TGCTTCCTGA TCTGACTCTG GAAGCTTGGC CATCAACAGA CAAGACCAAG
CGGGAGGAAG TGGATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25531
Clone ID Related Accession (Same CDS sequence) NM_001142.2
Accession Version NM_001142.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 576bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-16
Organism Homo sapiens(Human)
Product amelogenin, X isoform isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M86932.1. On Jan 19, 2000 this sequence version replaced NM_001142.1. Transcript Variant: This variant (1) lacks an in-frame exon in the coding region, compared to variant 3. Isoform 1 is shorter than isoform 3 and is the most abundant isoform of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
ATGGGGACCT GGATTTTATT TGCCTGCCTC CTGGGAGCAG CTTTTGCCAT GCCTCTACCA 
CCTCATCCTG GGCACCCTGG TTATATCAAC TTCAGCTATG AGGTGCTTAC CCCTTTGAAG
TGGTACCAGA GCATAAGGCC ACCGTACCCT TCCTATGGTT ACGAGCCCAT GGGTGGATGG
CTGCACCACC AAATCATCCC CGTGCTGTCC CAACAGCACC CCCCGACTCA CACCCTGCAG
CCTCATCACC ACATCCCAGT GGTGCCAGCT CAGCAGCCCG TGATCCCCCA GCAACCAATG
ATGCCCGTTC CTGGCCAACA CTCCATGACT CCAATCCAAC ACCACCAGCC AAACCTCCCT
CCGCCCGCCC AGCAGCCCTA CCAGCCCCAG CCTGTTCAGC CACAGCCTCA CCAGCCCATG
CAGCCCCAGC CACCTGTGCA CCCCATGCAG CCCCTGCCGC CACAGCCACC TCTGCCTCCG
ATGTTCCCCA TGCAGCCCCT GCCTCCCATG CTTCCTGATC TGACTCTGGA AGCTTGGCCA
TCAACAGACA AGACCAAGCG GGAGGAAGTG GATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001133.1
CDS69..644
Misc Feature(1)42..44
Misc Feature(2)150..641
Misc Feature(3)162..164
Exon (1)1..56
Exon (2)57..122
Exon (3)123..170
Exon (4)171..212
Exon (5)213..638
Exon (6)639..793
Translation

Target ORF information:

RefSeq Version NM_001142.2
Organism Homo sapiens(Human)
Definition Homo sapiens amelogenin, X-linked (AMELX), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001142.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGGACCT GGATTTTATT TGCCTGCCTC CTGGGAGCAG CTTTTGCCAT GCCTCTACCA 
CCTCATCCTG GGCACCCTGG TTATATCAAC TTCAGCTATG AGGTGCTTAC CCCTTTGAAG
TGGTACCAGA GCATAAGGCC ACCGTACCCT TCCTATGGTT ACGAGCCCAT GGGTGGATGG
CTGCACCACC AAATCATCCC CGTGCTGTCC CAACAGCACC CCCCGACTCA CACCCTGCAG
CCTCATCACC ACATCCCAGT GGTGCCAGCT CAGCAGCCCG TGATCCCCCA GCAACCAATG
ATGCCCGTTC CTGGCCAACA CTCCATGACT CCAATCCAAC ACCACCAGCC AAACCTCCCT
CCGCCCGCCC AGCAGCCCTA CCAGCCCCAG CCTGTTCAGC CACAGCCTCA CCAGCCCATG
CAGCCCCAGC CACCTGTGCA CCCCATGCAG CCCCTGCCGC CACAGCCACC TCTGCCTCCG
ATGTTCCCCA TGCAGCCCCT GCCTCCCATG CTTCCTGATC TGACTCTGGA AGCTTGGCCA
TCAACAGACA AGACCAAGCG GGAGGAAGTG GATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu99236
Clone ID Related Accession (Same CDS sequence) XM_017029404.1
Accession Version XM_017029404.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 585bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product amelogenin, X isoform isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
ATGGGGACCT GGATTTTATT TGCCTGCCTC CTGGGAGCAG CTTTTGCCAT GCCTCTACCA 
CCTCATCCTG GGCACCCTGG TTATATCAAC TTCAGCTATG AGGTGCTTAC CCCTTTGAAG
TGGTACCAGA GCATAAGGCC ACCGTACCCT TCCTATGGTT ACGAGCCCAT GGGTGGATGG
CTGCACCACC AAATCATCCC CGTGCTGTCC CAACAGCACC CCCCGACTCA CACCCTGCAG
CCTCATCACC ACATCCCAGT GGTGCCAGCT CAGCAGCCCG TGATCCCCCA GCAACCAATG
ATGCCCGTTC CTGGCCAACA CTCCATGACT CCAATCCAAC ACCACCAGCC AAACCTCCCT
CCGCCCGCCC AGCAGCCCTA CCAGCCCCAG CCTGTTCAGC CACAGCCTCA CCAGCCCATG
CAGCCCCAGC CACCTGTGCA CCCCATGCAG CCCCTGCCGC CACAGCCACC TCTGCCTCCG
ATGTTCCCCA TGCAGCCCCT GCCTCCCATG CTTCCTGATC TGACTCTGGA AGCTTGGCCA
TCAACAGACA AGACCAAGCG GGAGGAAGTG GTGAGTATAT TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016884893.1
CDS1170..1754
Misc Feature(1)1251..1739
Translation

Target ORF information:

RefSeq Version XM_017029404.1
Organism Homo sapiens(Human)
Definition Homo sapiens amelogenin, X-linked (AMELX), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017029404.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGGACCT GGATTTTATT TGCCTGCCTC CTGGGAGCAG CTTTTGCCAT GCCTCTACCA 
CCTCATCCTG GGCACCCTGG TTATATCAAC TTCAGCTATG AGGTGCTTAC CCCTTTGAAG
TGGTACCAGA GCATAAGGCC ACCGTACCCT TCCTATGGTT ACGAGCCCAT GGGTGGATGG
CTGCACCACC AAATCATCCC CGTGCTGTCC CAACAGCACC CCCCGACTCA CACCCTGCAG
CCTCATCACC ACATCCCAGT GGTGCCAGCT CAGCAGCCCG TGATCCCCCA GCAACCAATG
ATGCCCGTTC CTGGCCAACA CTCCATGACT CCAATCCAAC ACCACCAGCC AAACCTCCCT
CCGCCCGCCC AGCAGCCCTA CCAGCCCCAG CCTGTTCAGC CACAGCCTCA CCAGCCCATG
CAGCCCCAGC CACCTGTGCA CCCCATGCAG CCCCTGCCGC CACAGCCACC TCTGCCTCCG
ATGTTCCCCA TGCAGCCCCT GCCTCCCATG CTTCCTGATC TGACTCTGGA AGCTTGGCCA
TCAACAGACA AGACCAAGCG GGAGGAAGTG GTGAGTATAT TTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Full-length amelogenin influences the differentiation of human dental pulp stem cells.
Stem cell research & therapy710(2016 Jan)
Frasheri I,Ern C,Diegritz C,Hickel R,Hristov M,Folwaczny M


book

Association of Amelogenin with High Caries Experience in Indian Children.
The Journal of clinical pediatric dentistry39(5)458-61(2015)
Saha R,Sood PB,Sandhu M,Diwaker A,Upadhyaye S


book

Amelogenin in Enamel Tissue Engineering.
Advances in experimental medicine and biology881237-54(2015)
Uskoković V


book

Enamel formation genes associated with dental erosive wear.
Caries research49(3)236-42(2015)
Søvik JB,Vieira AR,Tveit AB,Mulic A


book

A pituitary homeobox 2 (Pitx2):microRNA-200a-3p:β-catenin pathway converts mesenchymal cells to amelogenin-expressing dental epithelial cells.
The Journal of biological chemistry289(39)27327-41(2014 Sep)
Sharp T,Wang J,Li X,Cao H,Gao S,Moreno M,Amendt BA


 
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