BBC3 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following BBC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BBC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu19952 NM_014417.5
Latest version!
Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu19952 XM_006723141.3
Latest version!
Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu20051 NM_001127242.3
Latest version!
Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu19976 NM_001127240.3
Latest version!
Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $188.30
$269.00
OHu20134 NM_001127241.3
Latest version!
Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $69.30
$99.00
OHu67065 XM_011526722.2
Latest version!
Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $139.30
$199.00
View Old Accession Versions >>
OHu20051 NM_001127242.2 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu20134 NM_001127241.2 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $69.30
$99.00
OHu19952 XM_006723141.2 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu19952 NM_014417.4 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu19976 NM_001127240.2 Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $188.30
$269.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu19952
    Clone ID Related Accession (Same CDS sequence) NM_014417.5 , XM_006723141.3 , XM_006723141.2 , NM_014417.4
    Accession Version NM_014417.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-19
    Organism Homo sapiens(human)
    Product bcl-2-binding component 3 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008532.7. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_014417.4. Transcript Variant: This variant (4) has alternate exon structure at its 5' end and it thus differs in the 5' UTR and 5' coding region, compared to variant 2. The encoded isoform (4, also known as PUMA-alpha) has a distinct N-terminus and is longer than isoform 2. Isoform 4 also includes the C-terminal BH3 domain and can localize to the mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279869.944.1, DRR138517.347682.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 RefSeq Select criteria :: based on manual assertion, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

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    ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
    GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
    GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
    GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
    CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
    GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
    CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
    CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
    CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
    TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055232.1
    CDS287..868
    Translation

    Target ORF information:

    RefSeq Version NM_014417.5
    Organism Homo sapiens(human)
    Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014417.5

    ORF Insert Sequence:

    1
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    481
    541
    ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
    GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
    GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
    GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
    CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
    GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
    CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
    CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
    CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
    TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19952
    Clone ID Related Accession (Same CDS sequence) NM_014417.5 , XM_006723141.3 , XM_006723141.2 , NM_014417.4
    Accession Version NM_014417.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product bcl-2-binding component 3 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF332558.1, BG258126.1, U82987.1 and AA887822.1. On Dec 30, 2011 this sequence version replaced NM_014417.3. Transcript Variant: This variant (4) has alternate exon structure at its 5' end and it thus differs in the 5' UTR and 5' coding region, compared to variant 2. The encoded isoform (4, also known as PUMA-alpha) has a distinct N-terminus and is longer than isoform 2. Isoform 4 also includes the C-terminal BH3 domain and can localize to the mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF332558.1, ERR279869.944.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

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    541
    ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
    GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
    GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
    GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
    CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
    GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
    CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
    CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
    CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
    TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055232.1
    CDS282..863
    Translation

    Target ORF information:

    RefSeq Version NM_014417.4
    Organism Homo sapiens(human)
    Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014417.4

    ORF Insert Sequence:

    1
    61
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    181
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    301
    361
    421
    481
    541
    ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
    GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
    GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
    GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
    CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
    GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
    CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
    CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
    CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
    TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19952
    Clone ID Related Accession (Same CDS sequence) NM_014417.5 , XM_006723141.3 , XM_006723141.2 , NM_014417.4
    Accession Version XM_006723141.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product bcl-2-binding component 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006723141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
    GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
    GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
    GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
    CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
    GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
    CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
    CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
    CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
    TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006723204.1
    CDS233..814
    Translation

    Target ORF information:

    RefSeq Version XM_006723141.3
    Organism Homo sapiens(human)
    Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006723141.3

    ORF Insert Sequence:

    1
    61
    121
    181
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    301
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    421
    481
    541
    ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
    GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
    GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
    GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
    CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
    GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
    CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
    CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
    CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
    TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19952
    Clone ID Related Accession (Same CDS sequence) NM_014417.5 , XM_006723141.3 , XM_006723141.2 , NM_014417.4
    Accession Version XM_006723141.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 582bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product bcl-2-binding component 3 isoform X1
    Comment

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    541
    ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
    GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
    GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
    GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
    CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
    GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
    CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
    CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
    CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
    TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006723204.1
    Translation

    Target ORF information:

    RefSeq Version XM_006723141.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006723141.2

    ORF Insert Sequence:

    1
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    121
    181
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    301
    361
    421
    481
    541
    ATGGCCCGCG CACGCCAGGA GGGCAGCTCC CCGGAGCCCG TAGAGGGCCT GGCCCGCGAC 
    GGCCCGCGCC CCTTCCCGCT CGGCCGCCTG GTGCCCTCGG CAGTGTCCTG CGGCCTCTGC
    GAGCCCGGCC TGGCTGCCGC CCCCGCCGCC CCCACCCTGC TGCCCGCTGC CTACCTCTGC
    GCCCCCACCG CCCCACCCGC CGTCACCGCC GCCCTGGGGG GTTCCCGCTG GCCTGGGGGT
    CCCCGCAGCC GGCCCCGAGG CCCGCGCCCG GACGGTCCTC AGCCCTCGCT CTCGCTGGCG
    GAGCAGCACC TGGAGTCGCC CGTGCCCAGC GCCCCGGGGG CTCTGGCGGG CGGTCCCACC
    CAGGCGGCCC CGGGAGTCCG CGGGGAGGAG GAACAGTGGG CCCGGGAGAT CGGGGCCCAG
    CTGCGGCGGA TGGCGGACGA CCTCAACGCA CAGTACGAGC GGCGGAGACA AGAGGAGCAG
    CAGCGGCACC GCCCCTCACC CTGGAGGGTC CTGTACAATC TCATCATGGG ACTCCTGCCC
    TTACCCAGGG GCCACAGAGC CCCCGAGATG GAGCCCAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20051
    Clone ID Related Accession (Same CDS sequence) NM_001127242.2 , NM_001127242.3
    Accession Version NM_001127242.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product bcl-2-binding component 3 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF354656.1, BG258126.1, U82987.1 and AA887822.1. On Dec 30, 2011 this sequence version replaced NM_001127242.1. Transcript Variant: This variant (3) lacks two exons spanning the 5' and central coding regions, resulting in a frameshift in the 3' coding region, compared to variant 2. The encoded isoform (3, also known as PUMA-delta) has the same N-terminus but is otherwise distinct and shorter, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354656.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2151887 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
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    301
    ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
    ACGACGTGGG TCCCCTGCCA GATTTGTGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
    ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
    AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
    GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120714.1
    CDS165..470
    Translation

    Target ORF information:

    RefSeq Version NM_001127242.2
    Organism Homo sapiens(human)
    Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127242.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
    ACGACGTGGG TCCCCTGCCA GATTTGTGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
    ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
    AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
    GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20051
    Clone ID Related Accession (Same CDS sequence) NM_001127242.2 , NM_001127242.3
    Accession Version NM_001127242.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product bcl-2-binding component 3 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008532.7. On Jun 2, 2019 this sequence version replaced NM_001127242.2. Transcript Variant: This variant (3) lacks two exons spanning the 5' and central coding regions, resulting in a frameshift in the 3' coding region, compared to variant 2. The encoded isoform (3, also known as PUMA-delta) has the same N-terminus but is otherwise distinct and shorter, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354656.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2151887 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

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    ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
    ACGACGTGGG TCCCCTGCCA GATTTGTGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
    ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
    AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
    GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120714.1
    CDS66..371
    Translation

    Target ORF information:

    RefSeq Version NM_001127242.3
    Organism Homo sapiens(human)
    Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127242.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
    ACGACGTGGG TCCCCTGCCA GATTTGTGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
    ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
    AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
    GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19976
    Clone ID Related Accession (Same CDS sequence) NM_001127240.3 , NM_001127240.2
    Accession Version NM_001127240.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product bcl-2-binding component 3 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008532.7. On Jun 2, 2019 this sequence version replaced NM_001127240.2. Transcript Variant: This variant (1) includes an alternate exon in the 5' coding region, resulting in a frameshift for the remainder of the CDS, compared to variant 2. The encoded isoform (1, also known as PUMA-gamma) has the same N-terminus but is otherwise distinct and longer, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354654.1, ERR279827.7607.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
    ACGACGTGGG TCCCCTGCCA GATTTGTGGC CCCAGGGAGC GCCATGGCCC GCGCACGCCA
    GGAGGGCAGC TCCCCGGAGC CCGTAGAGGG CCTGGCCCGC GACGGCCCGC GCCCCTTCCC
    GCTCGGCCGC CTGGTGCCCT CGGCAGTGTC CTGCGGCCTC TGCGAGCCCG GCCTGGCTGC
    CGCCCCCGCC GCCCCCACCC TGCTGCCCGC TGCCTACCTC TGCGCCCCCA CCGCCCCACC
    CGCCGTCACC GCCGCCCTGG GGGGTTCCCG CTGGCCTGGG GGTCCCCGCA GCCGGCCCCG
    AGGCCCGCGC CCGGACGGTC CTCAGCCCTC GCTCTCGCTG GCGGAGCAGC ACCTGGAGTC
    GCCCGTGCCC AGCGCCCCGG GGGCTCTGGC GGGCGGTCCC ACCCAGGCGG CCCCGGGAGT
    CCGCGGGGAG GAGGAACAGT GGGCCCGGGA GATCGGGGCC CAGCTGCGGC GGATGGCGGA
    CGACCTCAAC GCACAGTACG AGCGGCGGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
    ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
    AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
    GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120712.1
    CDS66..851
    Translation

    Target ORF information:

    RefSeq Version NM_001127240.3
    Organism Homo sapiens(human)
    Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127240.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
    ACGACGTGGG TCCCCTGCCA GATTTGTGGC CCCAGGGAGC GCCATGGCCC GCGCACGCCA
    GGAGGGCAGC TCCCCGGAGC CCGTAGAGGG CCTGGCCCGC GACGGCCCGC GCCCCTTCCC
    GCTCGGCCGC CTGGTGCCCT CGGCAGTGTC CTGCGGCCTC TGCGAGCCCG GCCTGGCTGC
    CGCCCCCGCC GCCCCCACCC TGCTGCCCGC TGCCTACCTC TGCGCCCCCA CCGCCCCACC
    CGCCGTCACC GCCGCCCTGG GGGGTTCCCG CTGGCCTGGG GGTCCCCGCA GCCGGCCCCG
    AGGCCCGCGC CCGGACGGTC CTCAGCCCTC GCTCTCGCTG GCGGAGCAGC ACCTGGAGTC
    GCCCGTGCCC AGCGCCCCGG GGGCTCTGGC GGGCGGTCCC ACCCAGGCGG CCCCGGGAGT
    CCGCGGGGAG GAGGAACAGT GGGCCCGGGA GATCGGGGCC CAGCTGCGGC GGATGGCGGA
    CGACCTCAAC GCACAGTACG AGCGGCGGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
    ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
    AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
    GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19976
    Clone ID Related Accession (Same CDS sequence) NM_001127240.3 , NM_001127240.2
    Accession Version NM_001127240.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product bcl-2-binding component 3 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF354654.1, BG258126.1, U82987.1 and AA887822.1. This sequence is a reference standard in the RefSeqGene project. On Dec 30, 2011 this sequence version replaced NM_001127240.1. Transcript Variant: This variant (1) includes an alternate exon in the 5' coding region, resulting in a frameshift for the remainder of the CDS, compared to variant 2. The encoded isoform (1, also known as PUMA-gamma) has the same N-terminus but is otherwise distinct and longer, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354654.1, ERR279827.7607.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
    ACGACGTGGG TCCCCTGCCA GATTTGTGGC CCCAGGGAGC GCCATGGCCC GCGCACGCCA
    GGAGGGCAGC TCCCCGGAGC CCGTAGAGGG CCTGGCCCGC GACGGCCCGC GCCCCTTCCC
    GCTCGGCCGC CTGGTGCCCT CGGCAGTGTC CTGCGGCCTC TGCGAGCCCG GCCTGGCTGC
    CGCCCCCGCC GCCCCCACCC TGCTGCCCGC TGCCTACCTC TGCGCCCCCA CCGCCCCACC
    CGCCGTCACC GCCGCCCTGG GGGGTTCCCG CTGGCCTGGG GGTCCCCGCA GCCGGCCCCG
    AGGCCCGCGC CCGGACGGTC CTCAGCCCTC GCTCTCGCTG GCGGAGCAGC ACCTGGAGTC
    GCCCGTGCCC AGCGCCCCGG GGGCTCTGGC GGGCGGTCCC ACCCAGGCGG CCCCGGGAGT
    CCGCGGGGAG GAGGAACAGT GGGCCCGGGA GATCGGGGCC CAGCTGCGGC GGATGGCGGA
    CGACCTCAAC GCACAGTACG AGCGGCGGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
    ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
    AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
    GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120712.1
    CDS165..950
    Translation

    Target ORF information:

    RefSeq Version NM_001127240.2
    Organism Homo sapiens(human)
    Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127240.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
    ACGACGTGGG TCCCCTGCCA GATTTGTGGC CCCAGGGAGC GCCATGGCCC GCGCACGCCA
    GGAGGGCAGC TCCCCGGAGC CCGTAGAGGG CCTGGCCCGC GACGGCCCGC GCCCCTTCCC
    GCTCGGCCGC CTGGTGCCCT CGGCAGTGTC CTGCGGCCTC TGCGAGCCCG GCCTGGCTGC
    CGCCCCCGCC GCCCCCACCC TGCTGCCCGC TGCCTACCTC TGCGCCCCCA CCGCCCCACC
    CGCCGTCACC GCCGCCCTGG GGGGTTCCCG CTGGCCTGGG GGTCCCCGCA GCCGGCCCCG
    AGGCCCGCGC CCGGACGGTC CTCAGCCCTC GCTCTCGCTG GCGGAGCAGC ACCTGGAGTC
    GCCCGTGCCC AGCGCCCCGG GGGCTCTGGC GGGCGGTCCC ACCCAGGCGG CCCCGGGAGT
    CCGCGGGGAG GAGGAACAGT GGGCCCGGGA GATCGGGGCC CAGCTGCGGC GGATGGCGGA
    CGACCTCAAC GCACAGTACG AGCGGCGGAG ACAAGAGGAG CAGCAGCGGC ACCGCCCCTC
    ACCCTGGAGG GTCCTGTACA ATCTCATCAT GGGACTCCTG CCCTTACCCA GGGGCCACAG
    AGCCCCCGAG ATGGAGCCCA ATTAGGTGCC TGCACCCGCC CGGTGGACGT CAGGGACTCG
    GGGGGCAGGC CCCTCCCACC TCCTGACACC CTGGCCAGCG CGGGGGACTT TCTCTGCACC
    ATGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20134
    Clone ID Related Accession (Same CDS sequence) NM_001127241.3 , NM_001127241.2
    Accession Version NM_001127241.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product bcl-2-binding component 3 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008532.7. On Jun 2, 2019 this sequence version replaced NM_001127241.2. Transcript Variant: This variant (2) encodes isoform 2 (also known as PUMA-beta), which includes the C-terminal BH3 domain and can localize to the mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354655.1, SRR1163655.659098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
    ACGACGTGGG TCCCCTGCCA GATTTGTGGT CCTCAGCCCT CGCTCTCGCT GGCGGAGCAG
    CACCTGGAGT CGCCCGTGCC CAGCGCCCCG GGGGCTCTGG CGGGCGGTCC CACCCAGGCG
    GCCCCGGGAG TCCGCGGGGA GGAGGAACAG TGGGCCCGGG AGATCGGGGC CCAGCTGCGG
    CGGATGGCGG ACGACCTCAA CGCACAGTAC GAGCGGCGGA GACAAGAGGA GCAGCAGCGG
    CACCGCCCCT CACCCTGGAG GGTCCTGTAC AATCTCATCA TGGGACTCCT GCCCTTACCC
    AGGGGCCACA GAGCCCCCGA GATGGAGCCC AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120713.1
    CDS66..461
    Translation

    Target ORF information:

    RefSeq Version NM_001127241.3
    Organism Homo sapiens(human)
    Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127241.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
    ACGACGTGGG TCCCCTGCCA GATTTGTGGT CCTCAGCCCT CGCTCTCGCT GGCGGAGCAG
    CACCTGGAGT CGCCCGTGCC CAGCGCCCCG GGGGCTCTGG CGGGCGGTCC CACCCAGGCG
    GCCCCGGGAG TCCGCGGGGA GGAGGAACAG TGGGCCCGGG AGATCGGGGC CCAGCTGCGG
    CGGATGGCGG ACGACCTCAA CGCACAGTAC GAGCGGCGGA GACAAGAGGA GCAGCAGCGG
    CACCGCCCCT CACCCTGGAG GGTCCTGTAC AATCTCATCA TGGGACTCCT GCCCTTACCC
    AGGGGCCACA GAGCCCCCGA GATGGAGCCC AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20134
    Clone ID Related Accession (Same CDS sequence) NM_001127241.3 , NM_001127241.2
    Accession Version NM_001127241.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 396bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product bcl-2-binding component 3 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF354655.1, BG258126.1, U82987.1 and AA887822.1. On Dec 30, 2011 this sequence version replaced NM_001127241.1. Transcript Variant: This variant (2) encodes isoform 2 (also known as PUMA-beta), which includes the C-terminal BH3 domain and can localize to the mitochondria. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF354655.1, SRR1163655.659098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02400288, SAMN02400289 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 11463392 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
    ACGACGTGGG TCCCCTGCCA GATTTGTGGT CCTCAGCCCT CGCTCTCGCT GGCGGAGCAG
    CACCTGGAGT CGCCCGTGCC CAGCGCCCCG GGGGCTCTGG CGGGCGGTCC CACCCAGGCG
    GCCCCGGGAG TCCGCGGGGA GGAGGAACAG TGGGCCCGGG AGATCGGGGC CCAGCTGCGG
    CGGATGGCGG ACGACCTCAA CGCACAGTAC GAGCGGCGGA GACAAGAGGA GCAGCAGCGG
    CACCGCCCCT CACCCTGGAG GGTCCTGTAC AATCTCATCA TGGGACTCCT GCCCTTACCC
    AGGGGCCACA GAGCCCCCGA GATGGAGCCC AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001120713.1
    CDS165..560
    Translation

    Target ORF information:

    RefSeq Version NM_001127241.2
    Organism Homo sapiens(human)
    Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001127241.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGAAATTTG GCATGGGGTC TGCCCAGGCA TGTCCATGCC AGGTGCCCAG GGCTGCTTCC 
    ACGACGTGGG TCCCCTGCCA GATTTGTGGT CCTCAGCCCT CGCTCTCGCT GGCGGAGCAG
    CACCTGGAGT CGCCCGTGCC CAGCGCCCCG GGGGCTCTGG CGGGCGGTCC CACCCAGGCG
    GCCCCGGGAG TCCGCGGGGA GGAGGAACAG TGGGCCCGGG AGATCGGGGC CCAGCTGCGG
    CGGATGGCGG ACGACCTCAA CGCACAGTAC GAGCGGCGGA GACAAGAGGA GCAGCAGCGG
    CACCGCCCCT CACCCTGGAG GGTCCTGTAC AATCTCATCA TGGGACTCCT GCCCTTACCC
    AGGGGCCACA GAGCCCCCGA GATGGAGCCC AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu67065
    Clone ID Related Accession (Same CDS sequence) XM_011526722.2
    Accession Version XM_011526722.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product bcl-2-binding component 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011526722.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTGGGGT TTCTGACCCG TCGGGGTGTC TGCGCCCCAG GGAGCGCCAT GGCCCGCGCA 
    CGCCAGGAGG GCAGCTCCCC GGAGCCCGTA GAGGGCCTGG CCCGCGACGG CCCGCGCCCC
    TTCCCGCTCG GCCGCCTGGT GCCCTCGGCA GTGTCCTGCG GCCTCTGCGA GCCCGGCCTG
    GCTGCCGCCC CCGCCGCCCC CACCCTGCTG CCCGCTGCCT ACCTCTGCGC CCCCACCGCC
    CCACCCGCCG TCACCGCCGC CCTGGGGGGT TCCCGCTGGC CTGGGGGTCC CCGCAGCCGG
    CCCCGAGGCC CGCGCCCGGA CGGTCCTCAG CCCTCGCTCT CGCTGGCGGA GCAGCACCTG
    GAGTCGCCCG TGCCCAGCGC CCCGGGGGCT CTGGCGGGCG GTCCCACCCA GGCGGCCCCG
    GGAGTCCGCG GGGAGGAGGA ACAGTGGGCC CGGGAGATCG GGGCCCAGCT GCGGCGGATG
    GCGGACGACC TCAACGCACA GTACGAGCGG CGGAGACAAG AGGAGCAGCA GCGGCACCGC
    CCCTCACCCT GGAGGGTCCT GTACAATCTC ATCATGGGAC TCCTGCCCTT ACCCAGGGGC
    CACAGAGCCC CCGAGATGGA GCCCAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011525024.1
    CDS126..755
    Translation

    Target ORF information:

    RefSeq Version XM_011526722.2
    Organism Homo sapiens(human)
    Definition Homo sapiens BCL2 binding component 3 (BBC3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011526722.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGTTGGGGT TTCTGACCCG TCGGGGTGTC TGCGCCCCAG GGAGCGCCAT GGCCCGCGCA 
    CGCCAGGAGG GCAGCTCCCC GGAGCCCGTA GAGGGCCTGG CCCGCGACGG CCCGCGCCCC
    TTCCCGCTCG GCCGCCTGGT GCCCTCGGCA GTGTCCTGCG GCCTCTGCGA GCCCGGCCTG
    GCTGCCGCCC CCGCCGCCCC CACCCTGCTG CCCGCTGCCT ACCTCTGCGC CCCCACCGCC
    CCACCCGCCG TCACCGCCGC CCTGGGGGGT TCCCGCTGGC CTGGGGGTCC CCGCAGCCGG
    CCCCGAGGCC CGCGCCCGGA CGGTCCTCAG CCCTCGCTCT CGCTGGCGGA GCAGCACCTG
    GAGTCGCCCG TGCCCAGCGC CCCGGGGGCT CTGGCGGGCG GTCCCACCCA GGCGGCCCCG
    GGAGTCCGCG GGGAGGAGGA ACAGTGGGCC CGGGAGATCG GGGCCCAGCT GCGGCGGATG
    GCGGACGACC TCAACGCACA GTACGAGCGG CGGAGACAAG AGGAGCAGCA GCGGCACCGC
    CCCTCACCCT GGAGGGTCCT GTACAATCTC ATCATGGGAC TCCTGCCCTT ACCCAGGGGC
    CACAGAGCCC CCGAGATGGA GCCCAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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