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GLRA1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol GLRA1
Entrez Gene ID 2741
Full Name glycine receptor alpha 1
Synonyms HKPX1,STHE
General protein information
Preferred Names

glycine receptor alpha 1

Names

glycine receptor subunit alpha-1
glycine receptor 48 kDa subunit
glycine receptor strychnine-binding subunit

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

5

5q33.1

Summary The protein encoded by this gene is a subunit of a pentameric inhibitory glycine receptor, which mediates postsynaptic inhibition in the central nervous system. Defects in this gene are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Multiple transcript variants encoding different isoforms have been found. [provided by RefSeq, Dec 2015].
Disorder MIM:

138491

Disorder Html: Startle disease/hyperekplexia, autosomal dominant, 149400 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_001292000.1 NP_001278929.1 glycine receptor subunit alpha-1 isoform 3
NM_001146040.1 NP_001139512.1 glycine receptor subunit alpha-1 isoform 1 precursor
NM_000171.3 NP_000162.2 glycine receptor subunit alpha-1 isoform 2 precursor
XM_017009349.1 XP_016864838.1 glycine receptor subunit alpha-1 isoform X1



Caenorhabditis elegans (roundworm) avr-15 NP_001024077.1
Xenopus tropicalis (tropical clawed frog) glra1 XP_004912784.1
Macaca mulatta (rhesus monkey) GLRA1 XP_002804635.1
Drosophila melanogaster (fruit fly) GluClalpha NP_001262740.1
Caenorhabditis elegans (roundworm) avr-15 NP_001024076.1
Homo sapiens (human) GLRA1 NP_001139512.1
Pan troglodytes (chimpanzee) GLRA1 XP_001169235.1
Bos taurus (cattle) GLRA1 NP_776746.1
Mus musculus (house mouse) Glra1 NP_065238.2
Rattus norvegicus (Norway rat) Glra1 NP_037265.1
Caenorhabditis elegans (roundworm) avr-14 NP_001020963.1
Caenorhabditis elegans (roundworm) glc-3 NP_504441.1
Gallus gallus (chicken) GLRA1 XP_425210.3
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001434 XP_003435829.1
Caenorhabditis elegans (roundworm) glc-1 NP_507090.1
Canis lupus familiaris (dog) GLRA1 XP_003434572.1
Danio rerio (zebrafish) glra1 NP_571477.1


Related articles in PubMed

Length of the TM3-4 loop of the glycine receptor modulates receptor desensitization.
Langlhofer G, Janzen D, Meiselbach H, Villmann C
Neuroscience letters600176-81(2015 Jul)

Ethanol Modulation is Quantitatively Determined by the Transmembrane Domain of Human α1 Glycine Receptors.
Horani S, Stater EP, Corringer PJ, Trudell JR, Harris RA, Howard RJ
Alcoholism, clinical and experimental research39(6)962-8(2015 Jun)

Allosteric and hyperekplexic mutant phenotypes investigated on an α1 glycine receptor transmembrane structure.
Moraga-Cid G, Sauguet L, Huon C, Malherbe L, Girard-Blanc C, Petres S, Murail S, Taly A, Baaden M, Delarue M, Corringer PJ
Proceedings of the National Academy of Sciences of the United States of America112(9)2865-70(2015 Mar)

Correlating structural and energetic changes in glycine receptor activation.
Scott S, Lynch JW, Keramidas A
The Journal of biological chemistry290(9)5621-34(2015 Feb)

[GLYCINE RECEPTOR: MOLECULAR ORGANIZATION AND PATHOLOGY].
Maleeva GV, Bregestovski PD
Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)61(5)107-17(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The E103K startle mutation reduces the sensitivity of glycine receptor alpha 1 to both glycine and sarcosine and impairs channel gating.
Title: The Startle Disease Mutation E103K Impairs Activation of Human Homomeric α1 Glycine Receptors by Disrupting an Intersubunit Salt Bridge across the Agonist Binding Site.

Results support the notion that the efficacies of ligands for the alpha1 GlyR are determined by conformational changes that occur within and near the ligand-binding domain: disruption of the D97-R119 interaction may be an important element in receptor activation and that agonists may break this putative intersubunit bond in order to destabilize the initial shut state and facilitate transitions towards the open state.
Title: Disruption of a putative intersubunit electrostatic bond enhances agonist efficacy at the human α1 glycine receptor.

gain-of-function GLRA1 mutations also cause hyperekplexia, although the mechanism is unknown. Here we identify two new gain-of-function mutations (I43F and W170S) and characterize these along with known gain-of-function mutations (Q226E, V280M, and R414H) to identify how they cause hyperekplexia.
Title: Investigating the Mechanism by Which Gain-of-function Mutations to the α1 Glycine Receptor Cause Hyperekplexia.

the TM3-4 loop length is critical for glycine receptor alpha1 desensitization and a direct neighborhood of both basic stretches changes receptor properties from non-desensitizing to desensitizing.
Title: Length of the TM3-4 loop of the glycine receptor modulates receptor desensitization.

GLRA1 and GLRB mutations are responsible for abnormal startled reactions in humans. (Review)
Title: [GLYCINE RECEPTOR: MOLECULAR ORGANIZATION AND PATHOLOGY].

The following GLRA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLRA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu26270
NM_001146040.1
Latest version!
Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu26374
NM_000171.3
Latest version!
Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu24622 NM_001292000.1
Latest version!
Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu95827 XM_017009349.1
Latest version!
Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26270
Clone ID Related Accession (Same CDS sequence) NM_001146040.1
Accession Version NM_001146040.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1374bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product glycine receptor subunit alpha-1 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X52009.1, BC114947.1 and AK226046.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC114947.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142670 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGA GCCCCATGTT GAATCTATTC
CAGGAGGATG AAGCTGGAGA AGGCCGCTTT AACTTCTCTG CCTATGGGAT GGGCCCAGCC
TGTCTACAGG CCAAGGATGG CATCTCAGTC AAGGGCGCCA ACAACAGTAA CACCACCAAC
CCCCCTCCTG CACCATCTAA GTCCCCAGAG GAGATGCGAA AACTCTTCAT CCAGAGGGCC
AAGAAGATCG ACAAAATATC CCGCATTGGC TTCCCCATGG CCTTCCTCAT TTTCAACATG
TTCTACTGGA TCATCTACAA GATTGTCCGT AGAGAGGACG TCCACAACCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001139512.1
CDS288..1661
Misc Feature(1)282..284
Misc Feature(2)306..1625
Misc Feature(3)411..1031
Misc Feature(4)975..992
Misc Feature(5)1038..1103
Misc Feature(6)1050..1616
Misc Feature(7)1119..1181
Misc Feature(8)1152..1154
Misc Feature(9)1215..1277
Misc Feature(10)1563..1625
Exon (1)1..343
Exon (2)344..471
Exon (3)472..539
Exon (4)540..763
Exon (5)764..846
Exon (6)847..984
Exon (7)985..1199
Exon (8)1200..1346
Exon (9)1347..1821
Translation

Target ORF information:

RefSeq Version NM_001146040.1
Organism Homo sapiens(Human)
Definition Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001146040.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGA GCCCCATGTT GAATCTATTC
CAGGAGGATG AAGCTGGAGA AGGCCGCTTT AACTTCTCTG CCTATGGGAT GGGCCCAGCC
TGTCTACAGG CCAAGGATGG CATCTCAGTC AAGGGCGCCA ACAACAGTAA CACCACCAAC
CCCCCTCCTG CACCATCTAA GTCCCCAGAG GAGATGCGAA AACTCTTCAT CCAGAGGGCC
AAGAAGATCG ACAAAATATC CCGCATTGGC TTCCCCATGG CCTTCCTCAT TTTCAACATG
TTCTACTGGA TCATCTACAA GATTGTCCGT AGAGAGGACG TCCACAACCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26374
Clone ID Related Accession (Same CDS sequence) NM_000171.3
Accession Version NM_000171.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product glycine receptor subunit alpha-1 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X52009.1, AK312702.1, BC074980.2 and AK226046.1. On Mar 26, 2009 this sequence version replaced NM_000171.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 5' end of the last exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X52009.1, AK312702.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142670 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGG AGGATGAAGC TGGAGAAGGC
CGCTTTAACT TCTCTGCCTA TGGGATGGGC CCAGCCTGTC TACAGGCCAA GGATGGCATC
TCAGTCAAGG GCGCCAACAA CAGTAACACC ACCAACCCCC CTCCTGCACC ATCTAAGTCC
CCAGAGGAGA TGCGAAAACT CTTCATCCAG AGGGCCAAGA AGATCGACAA AATATCCCGC
ATTGGCTTCC CCATGGCCTT CCTCATTTTC AACATGTTCT ACTGGATCAT CTACAAGATT
GTCCGTAGAG AGGACGTCCA CAACCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000162.2
CDS288..1637
Misc Feature(1)282..284
Misc Feature(2)306..1601
Misc Feature(3)411..1031
Misc Feature(4)975..992
Misc Feature(5)1038..1103
Misc Feature(6)1050..1592
Misc Feature(7)1119..1181
Misc Feature(8)1152..1154
Misc Feature(9)1215..1277
Misc Feature(10)1539..1601
Misc Feature(11)1542..1544
Exon (1)1..343
Exon (2)344..471
Exon (3)472..539
Exon (4)540..763
Exon (5)764..846
Exon (6)847..984
Exon (7)985..1199
Exon (8)1200..1346
Exon (9)1347..1797
Translation

Target ORF information:

RefSeq Version NM_000171.3
Organism Homo sapiens(Human)
Definition Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000171.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGG AGGATGAAGC TGGAGAAGGC
CGCTTTAACT TCTCTGCCTA TGGGATGGGC CCAGCCTGTC TACAGGCCAA GGATGGCATC
TCAGTCAAGG GCGCCAACAA CAGTAACACC ACCAACCCCC CTCCTGCACC ATCTAAGTCC
CCAGAGGAGA TGCGAAAACT CTTCATCCAG AGGGCCAAGA AGATCGACAA AATATCCCGC
ATTGGCTTCC CCATGGCCTT CCTCATTTTC AACATGTTCT ACTGGATCAT CTACAAGATT
GTCCGTAGAG AGGACGTCCA CAACCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24622
Clone ID Related Accession (Same CDS sequence) NM_001292000.1
Accession Version NM_001292000.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1101bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product glycine receptor subunit alpha-1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC114967.1, AK308539.1 and AK226046.1. On May 15, 2014 this sequence version replaced XM_005268411.1. Transcript Variant: This variant (3) lacks an internal exon in the 5' region, which results in translation initiation at a downstream AUG start codon, and contains an alternate splice site at the 5' end of the last exon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus and lacks an internal segment in the C-terminal region, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226046.1, BC114967.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142670 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGACTATA GGGTCAACAT CTTCCTGCGG CAGCAATGGA ACGACCCCCG CCTGGCCTAT 
AATGAATACC CTGACGACTC TCTGGACCTG GACCCATCCA TGCTGGACTC CATCTGGAAA
CCTGACCTGT TCTTTGCCAA CGAGAAGGGG GCCCACTTCC ATGAGATCAC CACAGACAAC
AAATTGCTAA GGATCTCCCG GAATGGGAAT GTCCTCTACA GCATCAGAAT CACCCTGACA
CTGGCCTGCC CCATGGACTT GAAGAATTTC CCCATGGATG TCCAGACATG TATCATGCAA
CTGGAAAGCT TTGGATATAC GATGAATGAC CTCATCTTTG AGTGGCAGGA ACAGGGAGCC
GTGCAGGTAG CAGATGGACT AACTCTGCCC CAGTTTATCT TGAAGGAAGA GAAGGACTTG
AGATACTGCA CCAAGCACTA CAACACAGGT AAATTCACCT GCATTGAGGC CCGGTTCCAC
CTGGAGCGGC AGATGGGTTA CTACCTGATT CAGATGTATA TTCCCAGCCT GCTCATTGTC
ATCCTCTCAT GGATCTCCTT CTGGATCAAC ATGGATGCTG CACCTGCTCG TGTGGGCCTA
GGCATCACCA CTGTGCTCAC CATGACCACC CAGAGCTCCG GCTCTCGAGC ATCTCTGCCC
AAGGTGTCCT ATGTGAAAGC CATTGACATT TGGATGGCAG TTTGCCTGCT CTTTGTGTTC
TCAGCCCTAT TAGAATATGC TGCCGTTAAC TTTGTGTCTC GGCAACATAA GGAGCTGCTC
CGATTCAGGA GGAAGCGGAG ACATCACAAG GAGGATGAAG CTGGAGAAGG CCGCTTTAAC
TTCTCTGCCT ATGGGATGGG CCCAGCCTGT CTACAGGCCA AGGATGGCAT CTCAGTCAAG
GGCGCCAACA ACAGTAACAC CACCAACCCC CCTCCTGCAC CATCTAAGTC CCCAGAGGAG
ATGCGAAAAC TCTTCATCCA GAGGGCCAAG AAGATCGACA AAATATCCCG CATTGGCTTC
CCCATGGCCT TCCTCATTTT CAACATGTTC TACTGGATCA TCTACAAGAT TGTCCGTAGA
GAGGACGTCC ACAACCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278929.1
CDS409..1509
Misc Feature(1)253..255
Misc Feature(2)409..1473
Misc Feature(3)<409..903
Misc Feature(4)922..1464
Exon (1)1..343
Exon (2)344..411
Exon (3)412..635
Exon (4)636..718
Exon (5)719..856
Exon (6)857..1071
Exon (7)1072..1218
Exon (8)1219..1669
Translation

Target ORF information:

RefSeq Version NM_001292000.1
Organism Homo sapiens(Human)
Definition Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001292000.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGACTATA GGGTCAACAT CTTCCTGCGG CAGCAATGGA ACGACCCCCG CCTGGCCTAT 
AATGAATACC CTGACGACTC TCTGGACCTG GACCCATCCA TGCTGGACTC CATCTGGAAA
CCTGACCTGT TCTTTGCCAA CGAGAAGGGG GCCCACTTCC ATGAGATCAC CACAGACAAC
AAATTGCTAA GGATCTCCCG GAATGGGAAT GTCCTCTACA GCATCAGAAT CACCCTGACA
CTGGCCTGCC CCATGGACTT GAAGAATTTC CCCATGGATG TCCAGACATG TATCATGCAA
CTGGAAAGCT TTGGATATAC GATGAATGAC CTCATCTTTG AGTGGCAGGA ACAGGGAGCC
GTGCAGGTAG CAGATGGACT AACTCTGCCC CAGTTTATCT TGAAGGAAGA GAAGGACTTG
AGATACTGCA CCAAGCACTA CAACACAGGT AAATTCACCT GCATTGAGGC CCGGTTCCAC
CTGGAGCGGC AGATGGGTTA CTACCTGATT CAGATGTATA TTCCCAGCCT GCTCATTGTC
ATCCTCTCAT GGATCTCCTT CTGGATCAAC ATGGATGCTG CACCTGCTCG TGTGGGCCTA
GGCATCACCA CTGTGCTCAC CATGACCACC CAGAGCTCCG GCTCTCGAGC ATCTCTGCCC
AAGGTGTCCT ATGTGAAAGC CATTGACATT TGGATGGCAG TTTGCCTGCT CTTTGTGTTC
TCAGCCCTAT TAGAATATGC TGCCGTTAAC TTTGTGTCTC GGCAACATAA GGAGCTGCTC
CGATTCAGGA GGAAGCGGAG ACATCACAAG GAGGATGAAG CTGGAGAAGG CCGCTTTAAC
TTCTCTGCCT ATGGGATGGG CCCAGCCTGT CTACAGGCCA AGGATGGCAT CTCAGTCAAG
GGCGCCAACA ACAGTAACAC CACCAACCCC CCTCCTGCAC CATCTAAGTC CCCAGAGGAG
ATGCGAAAAC TCTTCATCCA GAGGGCCAAG AAGATCGACA AAATATCCCG CATTGGCTTC
CCCATGGCCT TCCTCATTTT CAACATGTTC TACTGGATCA TCTACAAGAT TGTCCGTAGA
GAGGACGTCC ACAACCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu95827
Clone ID Related Accession (Same CDS sequence) XM_017009349.1
Accession Version XM_017009349.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1398bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product glycine receptor subunit alpha-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGGCATTC AACCAAACTT AGAGCCCACC TTAATAAAAG GCAACCTGTA CCAGCATGAG 
AAAGAGCCAT CATTAACCTT TTGCTCTCCT CTCTTCCTCC TTAGCCTTGC TGCTTCTAAG
GAGGCTGAAG CTGCTCGCTC CGCACCCAAG CCTATGTCAC CCTCGGATTT CCTGGATAAG
CTAATGGGGA GAACCTCCGG ATATGATGCC AGGATCAGGC CCAATTTTAA AGGTCCCCCA
GTGAACGTGA GCTGCAACAT TTTCATCAAC AGCTTTGGTT CCATTGCTGA GACAACCATG
GACTATAGGG TCAACATCTT CCTGCGGCAG CAATGGAACG ACCCCCGCCT GGCCTATAAT
GAATACCCTG ACGACTCTCT GGACCTGGAC CCATCCATGC TGGACTCCAT CTGGAAACCT
GACCTGTTCT TTGCCAACGA GAAGGGGGCC CACTTCCATG AGATCACCAC AGACAACAAA
TTGCTAAGGA TCTCCCGGAA TGGGAATGTC CTCTACAGCA TCAGAATCAC CCTGACACTG
GCCTGCCCCA TGGACTTGAA GAATTTCCCC ATGGATGTCC AGACATGTAT CATGCAACTG
GAAAGCTTTG GATATACGAT GAATGACCTC ATCTTTGAGT GGCAGGAACA GGGAGCCGTG
CAGGTAGCAG ATGGACTAAC TCTGCCCCAG TTTATCTTGA AGGAAGAGAA GGACTTGAGA
TACTGCACCA AGCACTACAA CACAGGTAAA TTCACCTGCA TTGAGGCCCG GTTCCACCTG
GAGCGGCAGA TGGGTTACTA CCTGATTCAG ATGTATATTC CCAGCCTGCT CATTGTCATC
CTCTCATGGA TCTCCTTCTG GATCAACATG GATGCTGCAC CTGCTCGTGT GGGCCTAGGC
ATCACCACTG TGCTCACCAT GACCACCCAG AGCTCCGGCT CTCGAGCATC TCTGCCCAAG
GTGTCCTATG TGAAAGCCAT TGACATTTGG ATGGCAGTTT GCCTGCTCTT TGTGTTCTCA
GCCCTATTAG AATATGCTGC CGTTAACTTT GTGTCTCGGC AACATAAGGA GCTGCTCCGA
TTCAGGAGGA AGCGGAGACA TCACAAGGAG GATGAAGCTG GAGAAGGCCG CTTTAACTTC
TCTGCCTATG GGATGGGCCC AGCCTGTCTA CAGGCCAAGG ATGGCATCTC AGTCAAGGGC
GCCAACAACA GTAACACCAC CAACCCCCCT CCTGCACCAT CTAAGTCCCC AGAGGAGATG
CGAAAACTCT TCATCCAGAG GGCCAAGAAG ATCGACAAAA TATCCCGCAT TGGCTTCCCC
ATGGCCTTCC TCATTTTCAA CATGTTCTAC TGGATCATCT ACAAGATTGT CCGTAGAGAG
GACGTCCACA ACCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016864838.1
CDS1..1398
Translation

Target ORF information:

RefSeq Version XM_017009349.1
Organism Homo sapiens(Human)
Definition Homo sapiens glycine receptor alpha 1 (GLRA1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017009349.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGGCATTC AACCAAACTT AGAGCCCACC TTAATAAAAG GCAACCTGTA CCAGCATGAG 
AAAGAGCCAT CATTAACCTT TTGCTCTCCT CTCTTCCTCC TTAGCCTTGC TGCTTCTAAG
GAGGCTGAAG CTGCTCGCTC CGCACCCAAG CCTATGTCAC CCTCGGATTT CCTGGATAAG
CTAATGGGGA GAACCTCCGG ATATGATGCC AGGATCAGGC CCAATTTTAA AGGTCCCCCA
GTGAACGTGA GCTGCAACAT TTTCATCAAC AGCTTTGGTT CCATTGCTGA GACAACCATG
GACTATAGGG TCAACATCTT CCTGCGGCAG CAATGGAACG ACCCCCGCCT GGCCTATAAT
GAATACCCTG ACGACTCTCT GGACCTGGAC CCATCCATGC TGGACTCCAT CTGGAAACCT
GACCTGTTCT TTGCCAACGA GAAGGGGGCC CACTTCCATG AGATCACCAC AGACAACAAA
TTGCTAAGGA TCTCCCGGAA TGGGAATGTC CTCTACAGCA TCAGAATCAC CCTGACACTG
GCCTGCCCCA TGGACTTGAA GAATTTCCCC ATGGATGTCC AGACATGTAT CATGCAACTG
GAAAGCTTTG GATATACGAT GAATGACCTC ATCTTTGAGT GGCAGGAACA GGGAGCCGTG
CAGGTAGCAG ATGGACTAAC TCTGCCCCAG TTTATCTTGA AGGAAGAGAA GGACTTGAGA
TACTGCACCA AGCACTACAA CACAGGTAAA TTCACCTGCA TTGAGGCCCG GTTCCACCTG
GAGCGGCAGA TGGGTTACTA CCTGATTCAG ATGTATATTC CCAGCCTGCT CATTGTCATC
CTCTCATGGA TCTCCTTCTG GATCAACATG GATGCTGCAC CTGCTCGTGT GGGCCTAGGC
ATCACCACTG TGCTCACCAT GACCACCCAG AGCTCCGGCT CTCGAGCATC TCTGCCCAAG
GTGTCCTATG TGAAAGCCAT TGACATTTGG ATGGCAGTTT GCCTGCTCTT TGTGTTCTCA
GCCCTATTAG AATATGCTGC CGTTAACTTT GTGTCTCGGC AACATAAGGA GCTGCTCCGA
TTCAGGAGGA AGCGGAGACA TCACAAGGAG GATGAAGCTG GAGAAGGCCG CTTTAACTTC
TCTGCCTATG GGATGGGCCC AGCCTGTCTA CAGGCCAAGG ATGGCATCTC AGTCAAGGGC
GCCAACAACA GTAACACCAC CAACCCCCCT CCTGCACCAT CTAAGTCCCC AGAGGAGATG
CGAAAACTCT TCATCCAGAG GGCCAAGAAG ATCGACAAAA TATCCCGCAT TGGCTTCCCC
ATGGCCTTCC TCATTTTCAA CATGTTCTAC TGGATCATCT ACAAGATTGT CCGTAGAGAG
GACGTCCACA ACCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Length of the TM3-4 loop of the glycine receptor modulates receptor desensitization.
Neuroscience letters600176-81(2015 Jul)
Langlhofer G,Janzen D,Meiselbach H,Villmann C


book

Ethanol Modulation is Quantitatively Determined by the Transmembrane Domain of Human α1 Glycine Receptors.
Alcoholism, clinical and experimental research39(6)962-8(2015 Jun)
Horani S,Stater EP,Corringer PJ,Trudell JR,Harris RA,Howard RJ


book

Allosteric and hyperekplexic mutant phenotypes investigated on an α1 glycine receptor transmembrane structure.
Proceedings of the National Academy of Sciences of the United States of America112(9)2865-70(2015 Mar)
Moraga-Cid G,Sauguet L,Huon C,Malherbe L,Girard-Blanc C,Petres S,Murail S,Taly A,Baaden M,Delarue M,Corringer PJ


book

Correlating structural and energetic changes in glycine receptor activation.
The Journal of biological chemistry290(9)5621-34(2015 Feb)
Scott S,Lynch JW,Keramidas A


book

[GLYCINE RECEPTOR: MOLECULAR ORGANIZATION AND PATHOLOGY].
Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)61(5)107-17(2015)
Maleeva GV,Bregestovski PD


 
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