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GLRA1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol GLRA1
Entrez Gene ID 2741
Full Name glycine receptor, alpha 1
Synonyms HKPX1, STHE
General protein information
Preferred Names
glycine receptor subunit alpha-1
Names
glycine receptor subunit alpha-1
glycine receptor 48 kDa subunit
glycine receptor strychnine-binding subunit
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

5

5q32

Summary The protein encoded by this gene is a subunit of a pentameric inhibitory glycine receptor. The receptor mediates postsynaptic inhibition in the central nervous system. Defects in this gene are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]. lac of sum
Disorder MIM:

138491

Disorder Html: Startle disease/hyperekplexia, autosomal dominant, 149400 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_001146040 NP_001139512 glycine receptor subunit alpha-1 isoform 1 precursor
XM_005268412 XP_005268469 glycine receptor subunit alpha-1 isoform X1
NM_000171 NP_000162 glycine receptor subunit alpha-1 isoform 2 precursor
NM_001292000 NP_001278929 glycine receptor subunit alpha-1 isoform 3



Homo sapiens (human) GLRA1 NP_001139512.1
Pan troglodytes (chimpanzee) GLRA1 XP_001169235.1
Macaca mulatta (Rhesus monkey) GLRA1 XP_002804635.1
Canis lupus familiaris (dog) GLRA1 XP_003434572.1
Bos taurus (cattle) GLRA1 NP_776746.1
Mus musculus (house mouse) Glra1 NP_065238.2
Rattus norvegicus (Norway rat) Glra1 NP_037265.1
Gallus gallus (chicken) GLRA1 XP_425210.3
Danio rerio (zebrafish) glra1 NP_571477.1
Drosophila melanogaster (fruit fly) GluClalpha NP_001262740.1
Caenorhabditis elegans avr-14 NP_001020963.1
Caenorhabditis elegans glc-3 NP_504441.1
Caenorhabditis elegans avr-15 NP_001024076.1
Caenorhabditis elegans avr-15 NP_001024077.1
Caenorhabditis elegans glc-1 NP_507090.1
Xenopus (Silurana) tropicalis (western clawed frog) glra1 XP_004912784.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GLRA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLRA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu26270
NM_001146040 Homo sapiens glycine receptor, alpha 1 (GLRA1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu39854 XM_005268412 PREDICTED: Homo sapiens glycine receptor, alpha 1 (GLRA1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu26374
NM_000171 Homo sapiens glycine receptor, alpha 1 (GLRA1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu24622 NM_001292000 Homo sapiens glycine receptor, alpha 1 (GLRA1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26270
Clone ID Related Accession (Same CDS sequence) NM_001146040
Accession Version NM_001146040.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1374bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 25-MAY-2014
Organism Homo sapiens (human)
Product glycine receptor subunit alpha-1 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X52009.1, BC114947.1 and AK226046.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC114947.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGA GCCCCATGTT GAATCTATTC
CAGGAGGATG AAGCTGGAGA AGGCCGCTTT AACTTCTCTG CCTATGGGAT GGGCCCAGCC
TGTCTACAGG CCAAGGATGG CATCTCAGTC AAGGGCGCCA ACAACAGTAA CACCACCAAC
CCCCCTCCTG CACCATCTAA GTCCCCAGAG GAGATGCGAA AACTCTTCAT CCAGAGGGCC
AAGAAGATCG ACAAAATATC CCGCATTGGC TTCCCCATGG CCTTCCTCAT TTTCAACATG
TTCTACTGGA TCATCTACAA GATTGTCCGT AGAGAGGACG TCCACAACCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001139512.1
CDS288..1661
Misc Feature(1)282..284(+)
Misc Feature(2)306..1625(+)
Misc Feature(3)411..1031(+)
Misc Feature(4)960..977(+)
Misc Feature(5)1029..1109(+)
Misc Feature(6)1050..1616(+)
Misc Feature(7)1128..1181(+)
Misc Feature(8)1224..1295(+)
Misc Feature(9)1572..1625(+)
Exon (1)1..343
Gene:GLRA1
Gene Synonym:
Exon (2)344..471
Gene:GLRA1
Gene Synonym:
Exon (3)472..539
Gene:GLRA1
Gene Synonym:
Exon (4)540..763
Gene:GLRA1
Gene Synonym:
Exon (5)764..846
Gene:GLRA1
Gene Synonym:
Exon (6)847..984
Gene:GLRA1
Gene Synonym:
Exon (7)985..1199
Gene:GLRA1
Gene Synonym:
Exon (8)1200..1346
Gene:GLRA1
Gene Synonym:
Exon (9)1347..1821
Gene:GLRA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001146040
Organism Homo sapiens (human)
Definition Homo sapiens glycine receptor, alpha 1 (GLRA1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001146040

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGA GCCCCATGTT GAATCTATTC
CAGGAGGATG AAGCTGGAGA AGGCCGCTTT AACTTCTCTG CCTATGGGAT GGGCCCAGCC
TGTCTACAGG CCAAGGATGG CATCTCAGTC AAGGGCGCCA ACAACAGTAA CACCACCAAC
CCCCCTCCTG CACCATCTAA GTCCCCAGAG GAGATGCGAA AACTCTTCAT CCAGAGGGCC
AAGAAGATCG ACAAAATATC CCGCATTGGC TTCCCCATGG CCTTCCTCAT TTTCAACATG
TTCTACTGGA TCATCTACAA GATTGTCCGT AGAGAGGACG TCCACAACCA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu39854
Clone ID Related Accession (Same CDS sequence) XM_005268412
Accession Version XM_005268412.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 924bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product glycine receptor subunit alpha-1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029289.12) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:530380479. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA
TCTCTGCCCA AGGGTCCGGT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005268469.1
CDS318..1241
Misc Feature(1)441..1061(+)
Misc Feature(2)1080..>1229(+)
Translation

Target ORF information:

RefSeq Version XM_005268412
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens glycine receptor, alpha 1 (GLRA1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005268412

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA
TCTCTGCCCA AGGGTCCGGT TTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu26374
Clone ID Related Accession (Same CDS sequence) NM_000171
Accession Version NM_000171.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1350bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glycine receptor subunit alpha-1 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X52009.1, AK312702.1, BC074980.2 and AK226046.1. On Mar 26, 2009 this sequence version replaced gi:119372309. Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 5' end of the last exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X52009.1, AK312702.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142670 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGG AGGATGAAGC TGGAGAAGGC
CGCTTTAACT TCTCTGCCTA TGGGATGGGC CCAGCCTGTC TACAGGCCAA GGATGGCATC
TCAGTCAAGG GCGCCAACAA CAGTAACACC ACCAACCCCC CTCCTGCACC ATCTAAGTCC
CCAGAGGAGA TGCGAAAACT CTTCATCCAG AGGGCCAAGA AGATCGACAA AATATCCCGC
ATTGGCTTCC CCATGGCCTT CCTCATTTTC AACATGTTCT ACTGGATCAT CTACAAGATT
GTCCGTAGAG AGGACGTCCA CAACCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000162.2
CDS288..1637
Misc Feature(1)282..284(+)
Misc Feature(2)306..1601(+)
Misc Feature(3)411..1031(+)
Misc Feature(4)960..977(+)
Misc Feature(5)1029..1109(+)
Misc Feature(6)1050..1592(+)
Misc Feature(7)1128..1181(+)
Misc Feature(8)1224..1295(+)
Misc Feature(9)1542..1544(+)
Misc Feature(10)1548..1601(+)
Exon (1)1..343
Gene:GLRA1
Gene Synonym:
Exon (2)344..471
Gene:GLRA1
Gene Synonym:
Exon (3)472..539
Gene:GLRA1
Gene Synonym:
Exon (4)540..763
Gene:GLRA1
Gene Synonym:
Exon (5)764..846
Gene:GLRA1
Gene Synonym:
Exon (6)847..984
Gene:GLRA1
Gene Synonym:
Exon (7)985..1199
Gene:GLRA1
Gene Synonym:
Exon (8)1200..1346
Gene:GLRA1
Gene Synonym:
Exon (9)1347..1797
Gene:GLRA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000171
Organism Homo sapiens (human)
Definition Homo sapiens glycine receptor, alpha 1 (GLRA1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000171

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGTACAGCT TCAATACTCT TCGACTCTAC CTTTGGGAGA CCATTGTATT CTTCAGCCTT 
GCTGCTTCTA AGGAGGCTGA AGCTGCTCGC TCCGCACCCA AGCCTATGTC ACCCTCGGAT
TTCCTGGATA AGCTAATGGG GAGAACCTCC GGATATGATG CCAGGATCAG GCCCAATTTT
AAAGGTCCCC CAGTGAACGT GAGCTGCAAC ATTTTCATCA ACAGCTTTGG TTCCATTGCT
GAGACAACCA TGGACTATAG GGTCAACATC TTCCTGCGGC AGCAATGGAA CGACCCCCGC
CTGGCCTATA ATGAATACCC TGACGACTCT CTGGACCTGG ACCCATCCAT GCTGGACTCC
ATCTGGAAAC CTGACCTGTT CTTTGCCAAC GAGAAGGGGG CCCACTTCCA TGAGATCACC
ACAGACAACA AATTGCTAAG GATCTCCCGG AATGGGAATG TCCTCTACAG CATCAGAATC
ACCCTGACAC TGGCCTGCCC CATGGACTTG AAGAATTTCC CCATGGATGT CCAGACATGT
ATCATGCAAC TGGAAAGCTT TGGATATACG ATGAATGACC TCATCTTTGA GTGGCAGGAA
CAGGGAGCCG TGCAGGTAGC AGATGGACTA ACTCTGCCCC AGTTTATCTT GAAGGAAGAG
AAGGACTTGA GATACTGCAC CAAGCACTAC AACACAGGTA AATTCACCTG CATTGAGGCC
CGGTTCCACC TGGAGCGGCA GATGGGTTAC TACCTGATTC AGATGTATAT TCCCAGCCTG
CTCATTGTCA TCCTCTCATG GATCTCCTTC TGGATCAACA TGGATGCTGC ACCTGCTCGT
GTGGGCCTAG GCATCACCAC TGTGCTCACC ATGACCACCC AGAGCTCCGG CTCTCGAGCA
TCTCTGCCCA AGGTGTCCTA TGTGAAAGCC ATTGACATTT GGATGGCAGT TTGCCTGCTC
TTTGTGTTCT CAGCCCTATT AGAATATGCT GCCGTTAACT TTGTGTCTCG GCAACATAAG
GAGCTGCTCC GATTCAGGAG GAAGCGGAGA CATCACAAGG AGGATGAAGC TGGAGAAGGC
CGCTTTAACT TCTCTGCCTA TGGGATGGGC CCAGCCTGTC TACAGGCCAA GGATGGCATC
TCAGTCAAGG GCGCCAACAA CAGTAACACC ACCAACCCCC CTCCTGCACC ATCTAAGTCC
CCAGAGGAGA TGCGAAAACT CTTCATCCAG AGGGCCAAGA AGATCGACAA AATATCCCGC
ATTGGCTTCC CCATGGCCTT CCTCATTTTC AACATGTTCT ACTGGATCAT CTACAAGATT
GTCCGTAGAG AGGACGTCCA CAACCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24622
Clone ID Related Accession (Same CDS sequence) NM_001292000
Accession Version NM_001292000.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1101bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product glycine receptor subunit alpha-1 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC114967.1, AK308539.1 and AK226046.1. On May 15, 2014 this sequence version replaced gi:530380477. Transcript Variant: This variant (3) lacks an internal exon in the 5' region, which results in translation initiation at a downstream AUG start codon, and contains an alternate splice site at the 5' end of the last exon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus and lacks an internal segment in the C-terminal region, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226046.1, BC114967.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2142670 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGACTATA GGGTCAACAT CTTCCTGCGG CAGCAATGGA ACGACCCCCG CCTGGCCTAT 
AATGAATACC CTGACGACTC TCTGGACCTG GACCCATCCA TGCTGGACTC CATCTGGAAA
CCTGACCTGT TCTTTGCCAA CGAGAAGGGG GCCCACTTCC ATGAGATCAC CACAGACAAC
AAATTGCTAA GGATCTCCCG GAATGGGAAT GTCCTCTACA GCATCAGAAT CACCCTGACA
CTGGCCTGCC CCATGGACTT GAAGAATTTC CCCATGGATG TCCAGACATG TATCATGCAA
CTGGAAAGCT TTGGATATAC GATGAATGAC CTCATCTTTG AGTGGCAGGA ACAGGGAGCC
GTGCAGGTAG CAGATGGACT AACTCTGCCC CAGTTTATCT TGAAGGAAGA GAAGGACTTG
AGATACTGCA CCAAGCACTA CAACACAGGT AAATTCACCT GCATTGAGGC CCGGTTCCAC
CTGGAGCGGC AGATGGGTTA CTACCTGATT CAGATGTATA TTCCCAGCCT GCTCATTGTC
ATCCTCTCAT GGATCTCCTT CTGGATCAAC ATGGATGCTG CACCTGCTCG TGTGGGCCTA
GGCATCACCA CTGTGCTCAC CATGACCACC CAGAGCTCCG GCTCTCGAGC ATCTCTGCCC
AAGGTGTCCT ATGTGAAAGC CATTGACATT TGGATGGCAG TTTGCCTGCT CTTTGTGTTC
TCAGCCCTAT TAGAATATGC TGCCGTTAAC TTTGTGTCTC GGCAACATAA GGAGCTGCTC
CGATTCAGGA GGAAGCGGAG ACATCACAAG GAGGATGAAG CTGGAGAAGG CCGCTTTAAC
TTCTCTGCCT ATGGGATGGG CCCAGCCTGT CTACAGGCCA AGGATGGCAT CTCAGTCAAG
GGCGCCAACA ACAGTAACAC CACCAACCCC CCTCCTGCAC CATCTAAGTC CCCAGAGGAG
ATGCGAAAAC TCTTCATCCA GAGGGCCAAG AAGATCGACA AAATATCCCG CATTGGCTTC
CCCATGGCCT TCCTCATTTT CAACATGTTC TACTGGATCA TCTACAAGAT TGTCCGTAGA
GAGGACGTCC ACAACCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001278929.1
CDS409..1509
Misc Feature(1)253..255(+)
Misc Feature(2)409..1473(+)
Misc Feature(3)409..903(+)
Misc Feature(4)922..1464(+)
Exon (1)1..343
Gene:GLRA1
Gene Synonym:
Exon (2)344..411
Gene:GLRA1
Gene Synonym:
Exon (3)412..635
Gene:GLRA1
Gene Synonym:
Exon (4)636..718
Gene:GLRA1
Gene Synonym:
Exon (5)719..856
Gene:GLRA1
Gene Synonym:
Exon (6)857..1071
Gene:GLRA1
Gene Synonym:
Exon (7)1072..1218
Gene:GLRA1
Gene Synonym:
Exon (8)1219..1669
Gene:GLRA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001292000
Organism Homo sapiens (human)
Definition Homo sapiens glycine receptor, alpha 1 (GLRA1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001292000

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGACTATA GGGTCAACAT CTTCCTGCGG CAGCAATGGA ACGACCCCCG CCTGGCCTAT 
AATGAATACC CTGACGACTC TCTGGACCTG GACCCATCCA TGCTGGACTC CATCTGGAAA
CCTGACCTGT TCTTTGCCAA CGAGAAGGGG GCCCACTTCC ATGAGATCAC CACAGACAAC
AAATTGCTAA GGATCTCCCG GAATGGGAAT GTCCTCTACA GCATCAGAAT CACCCTGACA
CTGGCCTGCC CCATGGACTT GAAGAATTTC CCCATGGATG TCCAGACATG TATCATGCAA
CTGGAAAGCT TTGGATATAC GATGAATGAC CTCATCTTTG AGTGGCAGGA ACAGGGAGCC
GTGCAGGTAG CAGATGGACT AACTCTGCCC CAGTTTATCT TGAAGGAAGA GAAGGACTTG
AGATACTGCA CCAAGCACTA CAACACAGGT AAATTCACCT GCATTGAGGC CCGGTTCCAC
CTGGAGCGGC AGATGGGTTA CTACCTGATT CAGATGTATA TTCCCAGCCT GCTCATTGTC
ATCCTCTCAT GGATCTCCTT CTGGATCAAC ATGGATGCTG CACCTGCTCG TGTGGGCCTA
GGCATCACCA CTGTGCTCAC CATGACCACC CAGAGCTCCG GCTCTCGAGC ATCTCTGCCC
AAGGTGTCCT ATGTGAAAGC CATTGACATT TGGATGGCAG TTTGCCTGCT CTTTGTGTTC
TCAGCCCTAT TAGAATATGC TGCCGTTAAC TTTGTGTCTC GGCAACATAA GGAGCTGCTC
CGATTCAGGA GGAAGCGGAG ACATCACAAG GAGGATGAAG CTGGAGAAGG CCGCTTTAAC
TTCTCTGCCT ATGGGATGGG CCCAGCCTGT CTACAGGCCA AGGATGGCAT CTCAGTCAAG
GGCGCCAACA ACAGTAACAC CACCAACCCC CCTCCTGCAC CATCTAAGTC CCCAGAGGAG
ATGCGAAAAC TCTTCATCCA GAGGGCCAAG AAGATCGACA AAATATCCCG CATTGGCTTC
CCCATGGCCT TCCTCATTTT CAACATGTTC TACTGGATCA TCTACAAGAT TGTCCGTAGA
GAGGACGTCC ACAACCAGTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

New hyperekplexia mutations provide insight into glycine receptor assembly, trafficking, and activation mechanisms
J. Biol. Chem. 288 (47), 33745-33759 (2013)
Bode A, Wood SE, Mullins JG, Keramidas A, Cushion TD, Thomas RH, Pickrell WO, Drew CJ, Masri A, Jones EA, Vassallo G, Born AP, Alehan F, Aharoni S, Bannasch G, Bartsch M, Kara B, Krause A, Karam EG, Matta S, Jain V, Mandel H, Freilinger M, Graham GE, Hobson E, Chatfield S, Vincent-Delorme C, Rahme JE, Afawi Z, Berkovic SF, Howell OW, Vanbellinghen JF, Rees MI, Chung SK and Lynch JW.


book

Analysis of hyperekplexia mutations identifies transmembrane domain rearrangements that mediate glycine receptor activation
J. Biol. Chem. 288 (47), 33760-33771 (2013)
Bode A and Lynch JW.


book

Open-channel structures of the human glycine receptor alpha1 full-length transmembrane domain
Structure 21 (10), 1897-1904 (2013)
Mowrey DD, Cui T, Jia Y, Ma D, Makhov AM, Zhang P, Tang P and Xu Y.


book

Glycine receptor autoimmune spectrum with stiff-man syndrome phenotype
JAMA Neurol 70 (1), 44-50 (2013)
McKeon A, Martinez-Hernandez E, Lancaster E, Matsumoto JY, Harvey RJ, McEvoy KM, Pittock SJ, Lennon VA and Dalmau J.


book

Inhibition of the ethanol-induced potentiation of alpha1 glycine receptor by a small peptide that interferes with Gbetagamma binding
J. Biol. Chem. 287 (48), 40713-40721 (2012)
San Martin L, Cerda F, Jimenez V, Fuentealba J, Munoz B, Aguayo LG and Guzman L.


book

Importance of Arg-219 for correct biogenesis of alpha 1 homooligomeric glycine receptors
FEBS Lett. 336 (3), 540-544 (1993)
Langosch D, Herbold A, Schmieden V, Borman J and Kirsch J.


book

Hyperekplexia
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Tijssen,M.A.J. and Rees,M.I.


book

Startle disease, or hyperekplexia: response to clonazepam and assignment of the gene (STHE) to chromosome 5q by linkage analysis
Ann. Neurol. 31 (6), 663-668 (1992)
Ryan SG, Sherman SL, Terry JC, Sparkes RS, Torres MC and Mackey RW.


book

Phosphorylation of the 48-kDa subunit of the glycine receptor by protein kinase C
J. Biol. Chem. 266 (1), 559-566 (1991)
Ruiz-Gomez A, Vaello ML, Valdivieso F and Mayor F Jr.


book

Alpha subunit variants of the human glycine receptor: primary structures, functional expression and chromosomal localization of the corresponding genes
EMBO J. 9 (3), 771-776 (1990)
Grenningloh G, Schmieden V, Schofield PR, Seeburg PH, Siddique T, Mohandas TK, Becker CM and Betz H.


 
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