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GNAI2 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol GNAI2
Entrez Gene ID 2771
Full Name G protein subunit alpha i2
Synonyms GIP,GNAI2B,H_LUCA15.1,H_LUCA16.1
General protein information
Preferred Names

G protein subunit alpha i2

Names

guanine nucleotide-binding protein G(i) subunit alpha-2
GTP-binding regulatory protein Gi alpha-2 chain
adenylate cyclase-inhibiting G alpha protein
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
guanine nucleotide-binding protein G(i), alpha-2 subunit

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

3

3p21.31

Summary The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013].
Disorder MIM:

139360

Disorder Html: Pituitary ACTH-secreting adenoma (3); Ventricular tachycardia,

mRNA and Protein(s)

mRNA Protein Name
NM_001282618.1 NP_001269547.1 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 4
NM_001166425.1 NP_001159897.1 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 2
NM_001282620.1 NP_001269549.1 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 6
NM_001282617.1 NP_001269546.1 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 3
NM_001282619.1 NP_001269548.1 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 5
NM_002070.3 NP_002061.1 guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 1



Gallus gallus (chicken) GNAI2 NP_990733.1
Canis lupus familiaris (dog) GNAI2 NP_001003364.1
Mus musculus (house mouse) Gnai2 NP_032164.2
Danio rerio (zebrafish) gnai2b NP_956136.1
Saccharomyces cerevisiae (baker's yeast) GPA1 NP_011868.1
Xenopus tropicalis (tropical clawed frog) gnai2 NP_989250.1
Macaca mulatta (rhesus monkey) GNAI2 XP_001103122.1
Homo sapiens (human) GNAI2 NP_002061.1
Pan troglodytes (chimpanzee) GNAI2 XP_001167942.1
Bos taurus (cattle) GNAI2 NP_001094510.1
Magnaporthe oryzae (rice blast fungus) MGG_00365 XP_003718715.1
Rattus norvegicus (Norway rat) Gnai2 NP_112297.1
Kluyveromyces lactis KLLA0F25916g XP_456234.1
Eremothecium gossypii AGOS_ADR153C NP_984250.1
Neurospora crassa NCU06493 XP_957133.1


Related articles in PubMed

The GPSM2/LGN GoLoco motifs are essential for hearing.
Bhonker Y, Abu-Rayyan A, Ushakov K, Amir-Zilberstein L, Shivatzki S, Yizhar-Barnea O, Elkan-Miller T, Tayeb-Fligelman E, Kim SM, Landau M, Kanaan M, Chen P, Matsuzaki F, Sprinzak D, Avraham KB
Mammalian genome : official journal of the International Mammalian Genome Society27(1-2)29-46(2016 Feb)

Close spatio-association of the transient receptor potential canonical 4 (TRPC4) channel with Gαi in TRPC4 activation process.
Myeong J, Kwak M, Jeon JP, Hong C, Jeon JH, So I
American journal of physiology. Cell physiology308(11)C879-89(2015 Jun)

Requirement of Gαi1/3-Gab1 signaling complex for keratinocyte growth factor-induced PI3K-AKT-mTORC1 activation.
Zhang YM, Zhang ZQ, Liu YY, Zhou X, Shi XH, Jiang Q, Fan DL, Cao C
The Journal of investigative dermatology135(1)181-191(2015 Jan)

Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network.
Chen TC, Lin KT, Chen CH, Lee SA, Lee PY, Liu YW, Kuo YL, Wang FS, Lai JM, Huang CY
Journal of proteome research13(12)5339-46(2014 Dec)

Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
Chiasserini D, van Weering JR, Piersma SR, Pham TV, Malekzadeh A, Teunissen CE, de Wit H, Jiménez CR
Journal of proteomics106191-204(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data indicate that, unlike in taste cells, TAS2Rs couple to the prevalent G proteins, Galphai1, Galphai2, and Galphai3, with no evidence for functional coupling to Galphagust.
Title: Coupling of Airway Smooth Muscle Bitter Taste Receptors to Intracellular Signaling and Relaxation Is via G

Kinocilium is essential for proper localization of Lgn, as well as Gai and aPKC, suggesting that cilium function plays a role in positioning of apical proteins critical for hearing.
Title: The GPSM2/LGN GoLoco motifs are essential for hearing.

Galpha(i2) activates the TRPC4 channel by direct binding.
Title: Close spatio-association of the transient receptor potential canonical 4 (TRPC4) channel with Gαi in TRPC4 activation process.

We observed increased expression of Galphai1/3 in wounded human skin and keloid skin tissues, suggesting the possible involvement of Galphai1/3 in wound healing and keloid formation.
Title: Requirement of Gαi1/3-Gab1 signaling complex for keratinocyte growth factor-induced PI3K-AKT-mTORC1 activation.

Data strongly implicate GNAI2 as a critical regulator of oncogenesis and an upstream driver of cancer progression in ovarian carcinoma.
Title: Suppression of GNAI2 message in ovarian cancer.

The following GNAI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNAI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu13581 NM_001166425.1
Latest version!
Homo sapiens G protein subunit alpha i2 (GNAI2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$154.50-$216.30
$309.00
OHu14225 NM_001282619.1
Latest version!
Homo sapiens G protein subunit alpha i2 (GNAI2), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu14246 NM_001282617.1
Latest version!
Homo sapiens G protein subunit alpha i2 (GNAI2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu14271 NM_001282618.1
Latest version!
Homo sapiens G protein subunit alpha i2 (GNAI2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu14309 NM_001282620.1
Latest version!
Homo sapiens G protein subunit alpha i2 (GNAI2), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu20814 NM_002070.3
Latest version!
Homo sapiens G protein subunit alpha i2 (GNAI2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00

ORF Online Only Promotion

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2-4 Clone 40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu13581
Clone ID Related Accession (Same CDS sequence) NM_001166425.1
Accession Version NM_001166425.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 957bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB044421.1, AK302330.1, AK126708.1 and AK055574.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302330.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGAGGTG CTGGGGAGTC AGGGAAGAGC ACCATCGTCA AGCAGATGAA GATCATCCAC 
GAGGATGGCT ACTCCGAGGA GGAATGCCGG CAGTACCGGG CGGTTGTCTA CAGCAACACC
ATCCAGTCCA TCATGGCCAT TGTCAAAGCC ATGGGCAACC TGCAGATCGA CTTTGCCGAC
CCCTCCAGAG CGGACGACGC CAGGCAGCTA TTTGCACTGT CCTGCACCGC CGAGGAGCAA
GGCGTGCTCC CTGATGACCT GTCCGGCGTC ATCCGGAGGC TCTGGGCTGA CCATGGTGTG
CAGGCCTGCT TTGGCCGCTC AAGGGAATAC CAGCTCAACG ACTCAGCTGC CTACTACCTG
AACGACCTGG AGCGTATTGC ACAGAGTGAC TACATCCCCA CACAGCAAGA TGTGCTACGG
ACCCGCGTAA AGACCACGGG GATCGTGGAG ACACACTTCA CCTTCAAGGA CCTACACTTC
AAGATGTTTG ATGTGGGTGG TCAGCGGTCT GAGCGGAAGA AGTGGATCCA CTGCTTTGAG
GGCGTCACAG CCATCATCTT CTGCGTAGCC TTGAGCGCCT ATGACTTGGT GCTAGCTGAG
GACGAGGAGA TGAACCGCAT GCATGAGAGC ATGAAGCTAT TCGATAGCAT CTGCAACAAC
AAGTGGTTCA CAGACACGTC CATCATCCTC TTCCTCAACA AGAAGGACCT GTTTGAGGAG
AAGATCACAC ACAGTCCCCT GACCATCTGC TTCCCTGAGT ACACAGGGGC CAACAAATAT
GATGAGGCAG CCAGCTACAT CCAGAGTAAG TTTGAGGACC TGAATAAGCG CAAAGACACC
AAGGAGATCT ACACGCACTT CACGTGCGCC ACCGACACCA AGAACGTGCA GTTCGTGTTT
GACGCCGTCA CCGATGTCAT CATCAAGAAC AACCTGAAGG ACTGCGGCCT CTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001159897.1
CDS176..1132
Misc Feature(1)137..139
Misc Feature(2)182..1123
Misc Feature(3)182..1111
Misc Feature(4)order(182..184,197..205,608..610,665..667,674..676,872..877,881..883,1040..1045)
Misc Feature(5)182..205
Misc Feature(6)order(185..193,275..277,287..289,299..301,308..310,317..322,374..376,383..385,512..514,599..601,674..676,698..700,710..712)
Misc Feature(7)593..619
Misc Feature(8)608..610
Misc Feature(9)order(611..613,617..619,623..625,656..658,662..664,674..682,686..688,695..700,704..715)
Misc Feature(10)order(617..619,692..694,701..706,713..715)
Misc Feature(11)665..676
Misc Feature(12)668..718
Misc Feature(13)872..883
Misc Feature(14)order(1016..1051,1055..1066)
Misc Feature(15)1040..1048
Exon (1)1..182
Exon (2)183..225
Exon (3)226..367
Exon (4)368..528
Exon (5)529..657
Exon (6)658..787
Exon (7)788..941
Exon (8)942..1156
Exon (9)1157..2143
Translation

Target ORF information:

RefSeq Version NM_001166425.1
Organism Homo sapiens(Human)
Definition Homo sapiens G protein subunit alpha i2 (GNAI2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001166425.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAGAGGTG CTGGGGAGTC AGGGAAGAGC ACCATCGTCA AGCAGATGAA GATCATCCAC 
GAGGATGGCT ACTCCGAGGA GGAATGCCGG CAGTACCGGG CGGTTGTCTA CAGCAACACC
ATCCAGTCCA TCATGGCCAT TGTCAAAGCC ATGGGCAACC TGCAGATCGA CTTTGCCGAC
CCCTCCAGAG CGGACGACGC CAGGCAGCTA TTTGCACTGT CCTGCACCGC CGAGGAGCAA
GGCGTGCTCC CTGATGACCT GTCCGGCGTC ATCCGGAGGC TCTGGGCTGA CCATGGTGTG
CAGGCCTGCT TTGGCCGCTC AAGGGAATAC CAGCTCAACG ACTCAGCTGC CTACTACCTG
AACGACCTGG AGCGTATTGC ACAGAGTGAC TACATCCCCA CACAGCAAGA TGTGCTACGG
ACCCGCGTAA AGACCACGGG GATCGTGGAG ACACACTTCA CCTTCAAGGA CCTACACTTC
AAGATGTTTG ATGTGGGTGG TCAGCGGTCT GAGCGGAAGA AGTGGATCCA CTGCTTTGAG
GGCGTCACAG CCATCATCTT CTGCGTAGCC TTGAGCGCCT ATGACTTGGT GCTAGCTGAG
GACGAGGAGA TGAACCGCAT GCATGAGAGC ATGAAGCTAT TCGATAGCAT CTGCAACAAC
AAGTGGTTCA CAGACACGTC CATCATCCTC TTCCTCAACA AGAAGGACCT GTTTGAGGAG
AAGATCACAC ACAGTCCCCT GACCATCTGC TTCCCTGAGT ACACAGGGGC CAACAAATAT
GATGAGGCAG CCAGCTACAT CCAGAGTAAG TTTGAGGACC TGAATAAGCG CAAAGACACC
AAGGAGATCT ACACGCACTT CACGTGCGCC ACCGACACCA AGAACGTGCA GTTCGTGTTT
GACGCCGTCA CCGATGTCAT CATCAAGAAC AACCTGAAGG ACTGCGGCCT CTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14225
Clone ID Related Accession (Same CDS sequence) NM_001282619.1
Accession Version NM_001282619.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA528337.1, BC016995.1 and AK055574.1. Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (5) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC016995.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGAGTATG CAGGGCATCT TCCTGCCAGC TCTGCCCAGG GCACCATATT GGCATGCACC 
AGCTGCACAG GTGCTGGGGA GTCAGGGAAG AGCACCATCG TCAAGCAGAT GAAGATCATC
CACGAGGATG GCTACTCCGA GGAGGAATGC CGGCAGTACC GGGCGGTTGT CTACAGCAAC
ACCATCCAGT CCATCATGGC CATTGTCAAA GCCATGGGCA ACCTGCAGAT CGACTTTGCC
GACCCCTCCA GAGCGGACGA CGCCAGGCAG CTATTTGCAC TGTCCTGCAC CGCCGAGGAG
CAAGGCGTGC TCCCTGATGA CCTGTCCGGC GTCATCCGGA GGCTCTGGGC TGACCATGGT
GTGCAGGCCT GCTTTGGCCG CTCAAGGGAA TACCAGCTCA ACGACTCAGC TGCCTACTAC
CTGAACGACC TGGAGCGTAT TGCACAGAGT GACTACATCC CCACACAGCA AGATGTGCTA
CGGACCCGCG TAAAGACCAC GGGGATCGTG GAGACACACT TCACCTTCAA GGACCTACAC
TTCAAGATGT TTGATGTGGG TGGTCAGCGG TCTGAGCGGA AGAAGTGGAT CCACTGCTTT
GAGGGCGTCA CAGCCATCAT CTTCTGCGTA GCCTTGAGCG CCTATGACTT GGTGCTAGCT
GAGGACGAGG AGATGAACCG CATGCATGAG AGCATGAAGC TATTCGATAG CATCTGCAAC
AACAAGTGGT TCACAGACAC GTCCATCATC CTCTTCCTCA ACAAGAAGGA CCTGTTTGAG
GAGAAGATCA CACACAGTCC CCTGACCATC TGCTTCCCTG AGTACACAGG GGCCAACAAA
TATGATGAGG CAGCCAGCTA CATCCAGAGT AAGTTTGAGG ACCTGAATAA GCGCAAAGAC
ACCAAGGAGA TCTACACGCA CTTCACGTGC GCCACCGACA CCAAGAACGT GCAGTTCGTG
TTTGACGCCG TCACCGATGT CATCATCAAG AACAACCTGA AGGACTGCGG CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269548.1
CDS561..1580
Misc Feature(1)519..521
Misc Feature(2)630..1571
Misc Feature(3)630..1559
Misc Feature(4)order(630..632,645..653,1056..1058,1113..1115,1122..1124,1320..1325,1329..1331,1488..1493)
Misc Feature(5)630..653
Misc Feature(6)order(633..641,723..725,735..737,747..749,756..758,765..770,822..824,831..833,960..962,1047..1049,1122..1124,1146..1148,1158..1160)
Misc Feature(7)1041..1067
Misc Feature(8)1056..1058
Misc Feature(9)order(1059..1061,1065..1067,1071..1073,1104..1106,1110..1112,1122..1130,1134..1136,1143..1148,1152..1163)
Misc Feature(10)order(1065..1067,1140..1142,1149..1154,1161..1163)
Misc Feature(11)1113..1124
Misc Feature(12)1116..1166
Misc Feature(13)1320..1331
Misc Feature(14)order(1464..1499,1503..1514)
Misc Feature(15)1488..1496
Exon (1)1..506
Exon (2)507..630
Exon (3)631..673
Exon (4)674..815
Exon (5)816..976
Exon (6)977..1105
Exon (7)1106..1235
Exon (8)1236..1389
Exon (9)1390..1604
Exon (10)1605..2591
Translation

Target ORF information:

RefSeq Version NM_001282619.1
Organism Homo sapiens(Human)
Definition Homo sapiens G protein subunit alpha i2 (GNAI2), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282619.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGAGTATG CAGGGCATCT TCCTGCCAGC TCTGCCCAGG GCACCATATT GGCATGCACC 
AGCTGCACAG GTGCTGGGGA GTCAGGGAAG AGCACCATCG TCAAGCAGAT GAAGATCATC
CACGAGGATG GCTACTCCGA GGAGGAATGC CGGCAGTACC GGGCGGTTGT CTACAGCAAC
ACCATCCAGT CCATCATGGC CATTGTCAAA GCCATGGGCA ACCTGCAGAT CGACTTTGCC
GACCCCTCCA GAGCGGACGA CGCCAGGCAG CTATTTGCAC TGTCCTGCAC CGCCGAGGAG
CAAGGCGTGC TCCCTGATGA CCTGTCCGGC GTCATCCGGA GGCTCTGGGC TGACCATGGT
GTGCAGGCCT GCTTTGGCCG CTCAAGGGAA TACCAGCTCA ACGACTCAGC TGCCTACTAC
CTGAACGACC TGGAGCGTAT TGCACAGAGT GACTACATCC CCACACAGCA AGATGTGCTA
CGGACCCGCG TAAAGACCAC GGGGATCGTG GAGACACACT TCACCTTCAA GGACCTACAC
TTCAAGATGT TTGATGTGGG TGGTCAGCGG TCTGAGCGGA AGAAGTGGAT CCACTGCTTT
GAGGGCGTCA CAGCCATCAT CTTCTGCGTA GCCTTGAGCG CCTATGACTT GGTGCTAGCT
GAGGACGAGG AGATGAACCG CATGCATGAG AGCATGAAGC TATTCGATAG CATCTGCAAC
AACAAGTGGT TCACAGACAC GTCCATCATC CTCTTCCTCA ACAAGAAGGA CCTGTTTGAG
GAGAAGATCA CACACAGTCC CCTGACCATC TGCTTCCCTG AGTACACAGG GGCCAACAAA
TATGATGAGG CAGCCAGCTA CATCCAGAGT AAGTTTGAGG ACCTGAATAA GCGCAAAGAC
ACCAAGGAGA TCTACACGCA CTTCACGTGC GCCACCGACA CCAAGAACGT GCAGTTCGTG
TTTGACGCCG TCACCGATGT CATCATCAAG AACAACCTGA AGGACTGCGG CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14246
Clone ID Related Accession (Same CDS sequence) NM_001282617.1
Accession Version NM_001282617.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 912bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK126708.1, AK096595.1 and AK055574.1. On Sep 14, 2013 this sequence version replaced XM_005265068.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK126708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGATCA TCCACGAGGA TGGCTACTCC GAGGAGGAAT GCCGGCAGTA CCGGGCGGTT 
GTCTACAGCA ACACCATCCA GTCCATCATG GCCATTGTCA AAGCCATGGG CAACCTGCAG
ATCGACTTTG CCGACCCCTC CAGAGCGGAC GACGCCAGGC AGCTATTTGC ACTGTCCTGC
ACCGCCGAGG AGCAAGGCGT GCTCCCTGAT GACCTGTCCG GCGTCATCCG GAGGCTCTGG
GCTGACCATG GTGTGCAGGC CTGCTTTGGC CGCTCAAGGG AATACCAGCT CAACGACTCA
GCTGCCTACT ACCTGAACGA CCTGGAGCGT ATTGCACAGA GTGACTACAT CCCCACACAG
CAAGATGTGC TACGGACCCG CGTAAAGACC ACGGGGATCG TGGAGACACA CTTCACCTTC
AAGGACCTAC ACTTCAAGAT GTTTGATGTG GGTGGTCAGC GGTCTGAGCG GAAGAAGTGG
ATCCACTGCT TTGAGGGCGT CACAGCCATC ATCTTCTGCG TAGCCTTGAG CGCCTATGAC
TTGGTGCTAG CTGAGGACGA GGAGATGAAC CGCATGCATG AGAGCATGAA GCTATTCGAT
AGCATCTGCA ACAACAAGTG GTTCACAGAC ACGTCCATCA TCCTCTTCCT CAACAAGAAG
GACCTGTTTG AGGAGAAGAT CACACACAGT CCCCTGACCA TCTGCTTCCC TGAGTACACA
GGGGCCAACA AATATGATGA GGCAGCCAGC TACATCCAGA GTAAGTTTGA GGACCTGAAT
AAGCGCAAAG ACACCAAGGA GATCTACACG CACTTCACGT GCGCCACCGA CACCAAGAAC
GTGCAGTTCG TGTTTGACGC CGTCACCGAT GTCATCATCA AGAACAACCT GAAGGACTGC
GGCCTCTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269546.1
CDS262..1173
Misc Feature(1)202..204
Misc Feature(2)262..1152
Misc Feature(3)262..1137
Misc Feature(4)order(316..318,328..330,340..342,349..351,358..363,415..417,424..426,553..555,640..642,715..717,739..741,751..753)
Misc Feature(5)634..660
Misc Feature(6)649..651
Misc Feature(7)order(652..654,658..660,664..666,697..699,703..705,715..723,727..729,736..741,745..756)
Misc Feature(8)order(658..660,733..735,742..747,754..756)
Misc Feature(9)706..717
Misc Feature(10)709..759
Misc Feature(11)913..924
Misc Feature(12)order(1057..1092,1096..1107)
Misc Feature(13)1081..1089
Exon (1)1..223
Exon (2)224..266
Exon (3)267..408
Exon (4)409..569
Exon (5)570..698
Exon (6)699..828
Exon (7)829..982
Exon (8)983..1197
Exon (9)1198..2184
Translation

Target ORF information:

RefSeq Version NM_001282617.1
Organism Homo sapiens(Human)
Definition Homo sapiens G protein subunit alpha i2 (GNAI2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282617.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGATCA TCCACGAGGA TGGCTACTCC GAGGAGGAAT GCCGGCAGTA CCGGGCGGTT 
GTCTACAGCA ACACCATCCA GTCCATCATG GCCATTGTCA AAGCCATGGG CAACCTGCAG
ATCGACTTTG CCGACCCCTC CAGAGCGGAC GACGCCAGGC AGCTATTTGC ACTGTCCTGC
ACCGCCGAGG AGCAAGGCGT GCTCCCTGAT GACCTGTCCG GCGTCATCCG GAGGCTCTGG
GCTGACCATG GTGTGCAGGC CTGCTTTGGC CGCTCAAGGG AATACCAGCT CAACGACTCA
GCTGCCTACT ACCTGAACGA CCTGGAGCGT ATTGCACAGA GTGACTACAT CCCCACACAG
CAAGATGTGC TACGGACCCG CGTAAAGACC ACGGGGATCG TGGAGACACA CTTCACCTTC
AAGGACCTAC ACTTCAAGAT GTTTGATGTG GGTGGTCAGC GGTCTGAGCG GAAGAAGTGG
ATCCACTGCT TTGAGGGCGT CACAGCCATC ATCTTCTGCG TAGCCTTGAG CGCCTATGAC
TTGGTGCTAG CTGAGGACGA GGAGATGAAC CGCATGCATG AGAGCATGAA GCTATTCGAT
AGCATCTGCA ACAACAAGTG GTTCACAGAC ACGTCCATCA TCCTCTTCCT CAACAAGAAG
GACCTGTTTG AGGAGAAGAT CACACACAGT CCCCTGACCA TCTGCTTCCC TGAGTACACA
GGGGCCAACA AATATGATGA GGCAGCCAGC TACATCCAGA GTAAGTTTGA GGACCTGAAT
AAGCGCAAAG ACACCAAGGA GATCTACACG CACTTCACGT GCGCCACCGA CACCAAGAAC
GTGCAGTTCG TGTTTGACGC CGTCACCGAT GTCATCATCA AGAACAACCT GAAGGACTGC
GGCCTCTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14271
Clone ID Related Accession (Same CDS sequence) NM_001282618.1
Accession Version NM_001282618.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 825bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY037331.1, BC012138.1, AK055574.1 and AK096595.1. Transcript Variant: This variant (4) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (4) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK055574.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCATTG TCAAAGCCAT GGGCAACCTG CAGATCGACT TTGCCGACCC CTCCAGAGCG 
GACGACGCCA GGCAGCTATT TGCACTGTCC TGCACCGCCG AGGAGCAAGG CGTGCTCCCT
GATGACCTGT CCGGCGTCAT CCGGAGGCTC TGGGCTGACC ATGGTGTGCA GGCCTGCTTT
GGCCGCTCAA GGGAATACCA GCTCAACGAC TCAGCTGCCT ACTACCTGAA CGACCTGGAG
CGTATTGCAC AGAGTGACTA CATCCCCACA CAGCAAGATG TGCTACGGAC CCGCGTAAAG
ACCACGGGGA TCGTGGAGAC ACACTTCACC TTCAAGGACC TACACTTCAA GATGTTTGAT
GTGGGTGGTC AGCGGTCTGA GCGGAAGAAG TGGATCCACT GCTTTGAGGG CGTCACAGCC
ATCATCTTCT GCGTAGCCTT GAGCGCCTAT GACTTGGTGC TAGCTGAGGA CGAGGAGATG
AACCGCATGC ATGAGAGCAT GAAGCTATTC GATAGCATCT GCAACAACAA GTGGTTCACA
GACACGTCCA TCATCCTCTT CCTCAACAAG AAGGACCTGT TTGAGGAGAA GATCACACAC
AGTCCCCTGA CCATCTGCTT CCCTGAGTAC ACAGGGGCCA ACAAATATGA TGAGGCAGCC
AGCTACATCC AGAGTAAGTT TGAGGACCTG AATAAGCGCA AAGACACCAA GGAGATCTAC
ACGCACTTCA CGTGCGCCAC CGACACCAAG AACGTGCAGT TCGTGTTTGA CGCCGTCACC
GATGTCATCA TCAAGAACAA CCTGAAGGAC TGCGGCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269547.1
CDS380..1204
Misc Feature(1)227..229
Misc Feature(2)<383..1195
Misc Feature(3)383..1183
Misc Feature(4)665..691
Misc Feature(5)680..682
Misc Feature(6)order(683..685,689..691,695..697,728..730,734..736,746..754,758..760,767..772,776..787)
Misc Feature(7)order(689..691,764..766,773..778,785..787)
Misc Feature(8)737..748
Misc Feature(9)740..790
Misc Feature(10)944..955
Misc Feature(11)order(1088..1123,1127..1138)
Misc Feature(12)1112..1120
Exon (1)1..297
Exon (2)298..439
Exon (3)440..600
Exon (4)601..729
Exon (5)730..859
Exon (6)860..1013
Exon (7)1014..1228
Exon (8)1229..2215
Translation

Target ORF information:

RefSeq Version NM_001282618.1
Organism Homo sapiens(Human)
Definition Homo sapiens G protein subunit alpha i2 (GNAI2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282618.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCCATTG TCAAAGCCAT GGGCAACCTG CAGATCGACT TTGCCGACCC CTCCAGAGCG 
GACGACGCCA GGCAGCTATT TGCACTGTCC TGCACCGCCG AGGAGCAAGG CGTGCTCCCT
GATGACCTGT CCGGCGTCAT CCGGAGGCTC TGGGCTGACC ATGGTGTGCA GGCCTGCTTT
GGCCGCTCAA GGGAATACCA GCTCAACGAC TCAGCTGCCT ACTACCTGAA CGACCTGGAG
CGTATTGCAC AGAGTGACTA CATCCCCACA CAGCAAGATG TGCTACGGAC CCGCGTAAAG
ACCACGGGGA TCGTGGAGAC ACACTTCACC TTCAAGGACC TACACTTCAA GATGTTTGAT
GTGGGTGGTC AGCGGTCTGA GCGGAAGAAG TGGATCCACT GCTTTGAGGG CGTCACAGCC
ATCATCTTCT GCGTAGCCTT GAGCGCCTAT GACTTGGTGC TAGCTGAGGA CGAGGAGATG
AACCGCATGC ATGAGAGCAT GAAGCTATTC GATAGCATCT GCAACAACAA GTGGTTCACA
GACACGTCCA TCATCCTCTT CCTCAACAAG AAGGACCTGT TTGAGGAGAA GATCACACAC
AGTCCCCTGA CCATCTGCTT CCCTGAGTAC ACAGGGGCCA ACAAATATGA TGAGGCAGCC
AGCTACATCC AGAGTAAGTT TGAGGACCTG AATAAGCGCA AAGACACCAA GGAGATCTAC
ACGCACTTCA CGTGCGCCAC CGACACCAAG AACGTGCAGT TCGTGTTTGA CGCCGTCACC
GATGTCATCA TCAAGAACAA CCTGAAGGAC TGCGGCCTCT TCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14309
Clone ID Related Accession (Same CDS sequence) NM_001282620.1
Accession Version NM_001282620.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA528337.1, AK096595.1 and AK055574.1. On Sep 14, 2013 this sequence version replaced XM_005265066.1. Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (6) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096595.1, AK096299.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGACGGAGG GTGTGAAGAC GCTAGGCTGG ACGAAGCAGA AAGGCGGGTG TCACTGGGGA 
CGTTCTGAGG GTGCTGGGGA GTCAGGGAAG AGCACCATCG TCAAGCAGAT GAAGATCATC
CACGAGGATG GCTACTCCGA GGAGGAATGC CGGCAGTACC GGGCGGTTGT CTACAGCAAC
ACCATCCAGT CCATCATGGC CATTGTCAAA GCCATGGGCA ACCTGCAGAT CGACTTTGCC
GACCCCTCCA GAGCGGACGA CGCCAGGCAG CTATTTGCAC TGTCCTGCAC CGCCGAGGAG
CAAGGCGTGC TCCCTGATGA CCTGTCCGGC GTCATCCGGA GGCTCTGGGC TGACCATGGT
GTGCAGGCCT GCTTTGGCCG CTCAAGGGAA TACCAGCTCA ACGACTCAGC TGCCTACTAC
CTGAACGACC TGGAGCGTAT TGCACAGAGT GACTACATCC CCACACAGCA AGATGTGCTA
CGGACCCGCG TAAAGACCAC GGGGATCGTG GAGACACACT TCACCTTCAA GGACCTACAC
TTCAAGATGT TTGATGTGGG TGGTCAGCGG TCTGAGCGGA AGAAGTGGAT CCACTGCTTT
GAGGGCGTCA CAGCCATCAT CTTCTGCGTA GCCTTGAGCG CCTATGACTT GGTGCTAGCT
GAGGACGAGG AGATGAACCG CATGCATGAG AGCATGAAGC TATTCGATAG CATCTGCAAC
AACAAGTGGT TCACAGACAC GTCCATCATC CTCTTCCTCA ACAAGAAGGA CCTGTTTGAG
GAGAAGATCA CACACAGTCC CCTGACCATC TGCTTCCCTG AGTACACAGG GGCCAACAAA
TATGATGAGG CAGCCAGCTA CATCCAGAGT AAGTTTGAGG ACCTGAATAA GCGCAAAGAC
ACCAAGGAGA TCTACACGCA CTTCACGTGC GCCACCGACA CCAAGAACGT GCAGTTCGTG
TTTGACGCCG TCACCGATGT CATCATCAAG AACAACCTGA AGGACTGCGG CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269549.1
CDS437..1456
Misc Feature(1)404..406
Misc Feature(2)506..1447
Misc Feature(3)506..1435
Misc Feature(4)order(506..508,521..529,932..934,989..991,998..1000,1196..1201,1205..1207,1364..1369)
Misc Feature(5)506..529
Misc Feature(6)order(509..517,599..601,611..613,623..625,632..634,641..646,698..700,707..709,836..838,923..925,998..1000,1022..1024,1034..1036)
Misc Feature(7)917..943
Misc Feature(8)932..934
Misc Feature(9)order(935..937,941..943,947..949,980..982,986..988,998..1006,1010..1012,1019..1024,1028..1039)
Misc Feature(10)order(941..943,1016..1018,1025..1030,1037..1039)
Misc Feature(11)989..1000
Misc Feature(12)992..1042
Misc Feature(13)1196..1207
Misc Feature(14)order(1340..1375,1379..1390)
Misc Feature(15)1364..1372
Exon (1)1..506
Exon (2)507..549
Exon (3)550..691
Exon (4)692..852
Exon (5)853..981
Exon (6)982..1111
Exon (7)1112..1265
Exon (8)1266..1480
Exon (9)1481..2467
Translation

Target ORF information:

RefSeq Version NM_001282620.1
Organism Homo sapiens(Human)
Definition Homo sapiens G protein subunit alpha i2 (GNAI2), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282620.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGACGGAGG GTGTGAAGAC GCTAGGCTGG ACGAAGCAGA AAGGCGGGTG TCACTGGGGA 
CGTTCTGAGG GTGCTGGGGA GTCAGGGAAG AGCACCATCG TCAAGCAGAT GAAGATCATC
CACGAGGATG GCTACTCCGA GGAGGAATGC CGGCAGTACC GGGCGGTTGT CTACAGCAAC
ACCATCCAGT CCATCATGGC CATTGTCAAA GCCATGGGCA ACCTGCAGAT CGACTTTGCC
GACCCCTCCA GAGCGGACGA CGCCAGGCAG CTATTTGCAC TGTCCTGCAC CGCCGAGGAG
CAAGGCGTGC TCCCTGATGA CCTGTCCGGC GTCATCCGGA GGCTCTGGGC TGACCATGGT
GTGCAGGCCT GCTTTGGCCG CTCAAGGGAA TACCAGCTCA ACGACTCAGC TGCCTACTAC
CTGAACGACC TGGAGCGTAT TGCACAGAGT GACTACATCC CCACACAGCA AGATGTGCTA
CGGACCCGCG TAAAGACCAC GGGGATCGTG GAGACACACT TCACCTTCAA GGACCTACAC
TTCAAGATGT TTGATGTGGG TGGTCAGCGG TCTGAGCGGA AGAAGTGGAT CCACTGCTTT
GAGGGCGTCA CAGCCATCAT CTTCTGCGTA GCCTTGAGCG CCTATGACTT GGTGCTAGCT
GAGGACGAGG AGATGAACCG CATGCATGAG AGCATGAAGC TATTCGATAG CATCTGCAAC
AACAAGTGGT TCACAGACAC GTCCATCATC CTCTTCCTCA ACAAGAAGGA CCTGTTTGAG
GAGAAGATCA CACACAGTCC CCTGACCATC TGCTTCCCTG AGTACACAGG GGCCAACAAA
TATGATGAGG CAGCCAGCTA CATCCAGAGT AAGTTTGAGG ACCTGAATAA GCGCAAAGAC
ACCAAGGAGA TCTACACGCA CTTCACGTGC GCCACCGACA CCAAGAACGT GCAGTTCGTG
TTTGACGCCG TCACCGATGT CATCATCAAG AACAACCTGA AGGACTGCGG CCTCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20814
Clone ID Related Accession (Same CDS sequence) NM_002070.3
Accession Version NM_002070.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1068bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product guanine nucleotide-binding protein G(i) subunit alpha-2 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY037331.1, BC012138.1, AK055574.1 and U73166.1. This sequence is a reference standard in the RefSeqGene project. On Sep 14, 2013 this sequence version replaced NM_002070.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012138.1, X04828.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCGGCCG AGCGCTCTAA GATGATCGAC 
AAGAACCTGC GGGAGGACGG AGAGAAGGCG GCGCGGGAGG TGAAGTTGCT GCTGTTGGGT
GCTGGGGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA CGAGGATGGC
TACTCCGAGG AGGAATGCCG GCAGTACCGG GCGGTTGTCT ACAGCAACAC CATCCAGTCC
ATCATGGCCA TTGTCAAAGC CATGGGCAAC CTGCAGATCG ACTTTGCCGA CCCCTCCAGA
GCGGACGACG CCAGGCAGCT ATTTGCACTG TCCTGCACCG CCGAGGAGCA AGGCGTGCTC
CCTGATGACC TGTCCGGCGT CATCCGGAGG CTCTGGGCTG ACCATGGTGT GCAGGCCTGC
TTTGGCCGCT CAAGGGAATA CCAGCTCAAC GACTCAGCTG CCTACTACCT GAACGACCTG
GAGCGTATTG CACAGAGTGA CTACATCCCC ACACAGCAAG ATGTGCTACG GACCCGCGTA
AAGACCACGG GGATCGTGGA GACACACTTC ACCTTCAAGG ACCTACACTT CAAGATGTTT
GATGTGGGTG GTCAGCGGTC TGAGCGGAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACA
GCCATCATCT TCTGCGTAGC CTTGAGCGCC TATGACTTGG TGCTAGCTGA GGACGAGGAG
ATGAACCGCA TGCATGAGAG CATGAAGCTA TTCGATAGCA TCTGCAACAA CAAGTGGTTC
ACAGACACGT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAGGA GAAGATCACA
CACAGTCCCC TGACCATCTG CTTCCCTGAG TACACAGGGG CCAACAAATA TGATGAGGCA
GCCAGCTACA TCCAGAGTAA GTTTGAGGAC CTGAATAAGC GCAAAGACAC CAAGGAGATC
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTCGTGTT TGACGCCGTC
ACCGATGTCA TCATCAAGAA CAACCTGAAG GACTGCGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002061.1
CDS180..1247
Misc Feature(1)216..1238
Misc Feature(2)279..1226
Misc Feature(3)order(297..299,312..320,723..725,780..782,789..791,987..992,996..998,1155..1160)
Misc Feature(4)297..320
Misc Feature(5)order(300..308,390..392,402..404,414..416,423..425,432..437,489..491,498..500,627..629,714..716,789..791,813..815,825..827)
Misc Feature(6)384..386
Misc Feature(7)708..734
Misc Feature(8)714..716
Misc Feature(9)723..725
Misc Feature(10)order(726..728,732..734,738..740,771..773,777..779,789..797,801..803,810..815,819..830)
Misc Feature(11)order(732..734,807..809,816..821,828..830)
Misc Feature(12)780..791
Misc Feature(13)783..833
Misc Feature(14)792..794
Misc Feature(15)987..998
Misc Feature(16)order(1131..1166,1170..1181)
Misc Feature(17)1155..1163
Misc Feature(18)1233..1235
Exon (1)1..297
Exon (2)298..340
Exon (3)341..482
Exon (4)483..643
Exon (5)644..772
Exon (6)773..902
Exon (7)903..1056
Exon (8)1057..1271
Exon (9)1272..2258
Translation

Target ORF information:

RefSeq Version NM_002070.3
Organism Homo sapiens(Human)
Definition Homo sapiens G protein subunit alpha i2 (GNAI2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002070.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCGGCCG AGCGCTCTAA GATGATCGAC 
AAGAACCTGC GGGAGGACGG AGAGAAGGCG GCGCGGGAGG TGAAGTTGCT GCTGTTGGGT
GCTGGGGAGT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA CGAGGATGGC
TACTCCGAGG AGGAATGCCG GCAGTACCGG GCGGTTGTCT ACAGCAACAC CATCCAGTCC
ATCATGGCCA TTGTCAAAGC CATGGGCAAC CTGCAGATCG ACTTTGCCGA CCCCTCCAGA
GCGGACGACG CCAGGCAGCT ATTTGCACTG TCCTGCACCG CCGAGGAGCA AGGCGTGCTC
CCTGATGACC TGTCCGGCGT CATCCGGAGG CTCTGGGCTG ACCATGGTGT GCAGGCCTGC
TTTGGCCGCT CAAGGGAATA CCAGCTCAAC GACTCAGCTG CCTACTACCT GAACGACCTG
GAGCGTATTG CACAGAGTGA CTACATCCCC ACACAGCAAG ATGTGCTACG GACCCGCGTA
AAGACCACGG GGATCGTGGA GACACACTTC ACCTTCAAGG ACCTACACTT CAAGATGTTT
GATGTGGGTG GTCAGCGGTC TGAGCGGAAG AAGTGGATCC ACTGCTTTGA GGGCGTCACA
GCCATCATCT TCTGCGTAGC CTTGAGCGCC TATGACTTGG TGCTAGCTGA GGACGAGGAG
ATGAACCGCA TGCATGAGAG CATGAAGCTA TTCGATAGCA TCTGCAACAA CAAGTGGTTC
ACAGACACGT CCATCATCCT CTTCCTCAAC AAGAAGGACC TGTTTGAGGA GAAGATCACA
CACAGTCCCC TGACCATCTG CTTCCCTGAG TACACAGGGG CCAACAAATA TGATGAGGCA
GCCAGCTACA TCCAGAGTAA GTTTGAGGAC CTGAATAAGC GCAAAGACAC CAAGGAGATC
TACACGCACT TCACGTGCGC CACCGACACC AAGAACGTGC AGTTCGTGTT TGACGCCGTC
ACCGATGTCA TCATCAAGAA CAACCTGAAG GACTGCGGCC TCTTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

The GPSM2/LGN GoLoco motifs are essential for hearing.
Mammalian genome : official journal of the International Mammalian Genome Society27(1-2)29-46(2016 Feb)
Bhonker Y,Abu-Rayyan A,Ushakov K,Amir-Zilberstein L,Shivatzki S,Yizhar-Barnea O,Elkan-Miller T,Tayeb-Fligelman E,Kim SM,Landau M,Kanaan M,Chen P,Matsuzaki F,Sprinzak D,Avraham KB


book

Close spatio-association of the transient receptor potential canonical 4 (TRPC4) channel with Gαi in TRPC4 activation process.
American journal of physiology. Cell physiology308(11)C879-89(2015 Jun)
Myeong J,Kwak M,Jeon JP,Hong C,Jeon JH,So I


book

Requirement of Gαi1/3-Gab1 signaling complex for keratinocyte growth factor-induced PI3K-AKT-mTORC1 activation.
The Journal of investigative dermatology135(1)181-191(2015 Jan)
Zhang YM,Zhang ZQ,Liu YY,Zhou X,Shi XH,Jiang Q,Fan DL,Cao C


book

Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network.
Journal of proteome research13(12)5339-46(2014 Dec)
Chen TC,Lin KT,Chen CH,Lee SA,Lee PY,Liu YW,Kuo YL,Wang FS,Lai JM,Huang CY


book

Proteomic analysis of cerebrospinal fluid extracellular vesicles: a comprehensive dataset.
Journal of proteomics106191-204(2014 Jun)
Chiasserini D,van Weering JR,Piersma SR,Pham TV,Malekzadeh A,Teunissen CE,de Wit H,Jiménez CR


 
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