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GNAI3 cDNA ORF clone, Homo sapiens (human)

Gene Symbol GNAI3
Entrez Gene ID 2773
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
Synonyms 87U6, ARCND1
General protein information
Preferred Names
guanine nucleotide-binding protein G(k) subunit alpha
Names
guanine nucleotide-binding protein G(k) subunit alpha
g(i) alpha-3
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p13

Summary Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]. lac of sum
Disorder MIM:

139370


mRNA and Protein(s)

mRNA Protein Name
NM_006496 NP_006487 guanine nucleotide-binding protein G(k) subunit alpha



Homo sapiens (human) GNAI3 NP_006487.1
Pan troglodytes (chimpanzee) GNAI3 XP_513624.1
Macaca mulatta (Rhesus monkey) GNAI3 XP_001091470.1
Canis lupus familiaris (dog) GNAI3 XP_854614.3
Bos taurus (cattle) GNAI3 NP_001029849.1
Mus musculus (house mouse) Gnai3 NP_034436.1
Rattus norvegicus (Norway rat) Gnai3 NP_037238.1
Gallus gallus (chicken) GNAI3 NP_989580.1
Danio rerio (zebrafish) gnai3 NP_001104720.1
Xenopus (Silurana) tropicalis (western clawed frog) gnai3 NP_001011471.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GNAI3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNAI3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19778
NM_006496 Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19778
Clone ID Related Accession (Same CDS sequence) NM_006496
Accession Version NM_006496.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1065bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(k) subunit alpha
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP289420.1, BC025285.1, BE886720.1, BU195094.1, AW984247.1, CX165017.1, BI492787.1, AI684171.1 and BU622030.1. This sequence is a reference standard in the RefSeqGene project. On Jun 14, 2012 this sequence version replaced gi:169646784. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M27543.1, BC025285.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCTGCA CGTTGAGCGC CGAAGACAAG GCGGCAGTGG AGCGAAGCAA GATGATCGAC 
CGCAACTTAC GGGAGGACGG GGAAAAAGCG GCCAAAGAAG TGAAGCTGCT GCTACTCGGT
GCTGGAGAAT CTGGTAAAAG CACCATTGTG AAACAGATGA AAATCATTCA TGAGGATGGC
TATTCAGAGG ATGAATGTAA ACAATATAAA GTAGTTGTCT ACAGCAATAC TATACAGTCC
ATCATTGCAA TCATAAGAGC CATGGGACGG CTAAAGATTG ACTTTGGGGA AGCTGCCAGG
GCAGATGATG CCCGGCAATT ATTTGTTTTA GCTGGCAGTG CTGAAGAAGG AGTCATGACT
CCAGAACTAG CAGGAGTGAT TAAACGGTTA TGGCGAGATG GTGGGGTACA AGCTTGCTTC
AGCAGATCCA GGGAATATCA GCTCAATGAT TCTGCTTCAT ATTATCTAAA TGATCTGGAT
AGAATATCCC AGTCTAACTA CATTCCAACT CAGCAAGATG TTCTTCGGAC GAGAGTGAAG
ACCACAGGCA TTGTAGAAAC ACATTTCACC TTCAAAGACC TATACTTCAA GATGTTTGAT
GTAGGTGGCC AAAGATCAGA ACGAAAAAAG TGGATTCACT GTTTTGAGGG AGTGACAGCA
ATTATCTTCT GTGTGGCCCT CAGTGATTAT GACCTTGTTC TGGCTGAGGA CGAGGAGATG
AACCGAATGC ATGAAAGCAT GAAACTGTTT GACAGCATTT GTAATAACAA ATGGTTTACA
GAAACTTCAA TCATTCTCTT CCTTAACAAG AAAGACCTTT TTGAGGAAAA AATAAAGAGG
AGTCCGTTAA CTATCTGTTA TCCAGAATAC ACAGGTTCCA ATACATATGA AGAGGCAGCT
GCCTATATTC AATGCCAGTT TGAAGATCTG AACAGAAGAA AAGATACCAA GGAGATCTAT
ACTCACTTCA CCTGTGCCAC AGACACGAAG AATGTGCAGT TTGTTTTTGA TGCTGTTACA
GATGTCATCA TTAAAAACAA CTTAAAGGAA TGTGGACTTT ATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006487.1
CDS158..1222
Misc Feature(1)122..124(+)
Misc Feature(2)197..1213(+)
Misc Feature(3)257..1201(+)
Misc Feature(4)275..1135(+)
Misc Feature(5)275..298(+)
Misc Feature(6)278..802(+)
Misc Feature(7)362..364(+)
Misc Feature(8)683..709(+)
Misc Feature(9)698..700(+)
Misc Feature(10)701..805(+)
Misc Feature(11)707..805(+)
Misc Feature(12)755..766(+)
Misc Feature(13)758..808(+)
Misc Feature(14)962..973(+)
Misc Feature(15)1106..1156(+)
Misc Feature(16)1130..1138(+)
Exon (1)1..275
Gene:GNAI3
Gene Synonym:
Exon (2)276..318
Gene:GNAI3
Gene Synonym:
Exon (3)319..460
Gene:GNAI3
Gene Synonym:
Exon (4)461..618
Gene:GNAI3
Gene Synonym:
Exon (5)619..747
Gene:GNAI3
Gene Synonym:
Exon (6)748..877
Gene:GNAI3
Gene Synonym:
Exon (7)878..1031
Gene:GNAI3
Gene Synonym:
Exon (8)1032..1244
Gene:GNAI3
Gene Synonym:
Exon (9)1245..4743
Gene:GNAI3
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_006496
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 (GNAI3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006496

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGGCTGCA CGTTGAGCGC CGAAGACAAG GCGGCAGTGG AGCGAAGCAA GATGATCGAC 
CGCAACTTAC GGGAGGACGG GGAAAAAGCG GCCAAAGAAG TGAAGCTGCT GCTACTCGGT
GCTGGAGAAT CTGGTAAAAG CACCATTGTG AAACAGATGA AAATCATTCA TGAGGATGGC
TATTCAGAGG ATGAATGTAA ACAATATAAA GTAGTTGTCT ACAGCAATAC TATACAGTCC
ATCATTGCAA TCATAAGAGC CATGGGACGG CTAAAGATTG ACTTTGGGGA AGCTGCCAGG
GCAGATGATG CCCGGCAATT ATTTGTTTTA GCTGGCAGTG CTGAAGAAGG AGTCATGACT
CCAGAACTAG CAGGAGTGAT TAAACGGTTA TGGCGAGATG GTGGGGTACA AGCTTGCTTC
AGCAGATCCA GGGAATATCA GCTCAATGAT TCTGCTTCAT ATTATCTAAA TGATCTGGAT
AGAATATCCC AGTCTAACTA CATTCCAACT CAGCAAGATG TTCTTCGGAC GAGAGTGAAG
ACCACAGGCA TTGTAGAAAC ACATTTCACC TTCAAAGACC TATACTTCAA GATGTTTGAT
GTAGGTGGCC AAAGATCAGA ACGAAAAAAG TGGATTCACT GTTTTGAGGG AGTGACAGCA
ATTATCTTCT GTGTGGCCCT CAGTGATTAT GACCTTGTTC TGGCTGAGGA CGAGGAGATG
AACCGAATGC ATGAAAGCAT GAAACTGTTT GACAGCATTT GTAATAACAA ATGGTTTACA
GAAACTTCAA TCATTCTCTT CCTTAACAAG AAAGACCTTT TTGAGGAAAA AATAAAGAGG
AGTCCGTTAA CTATCTGTTA TCCAGAATAC ACAGGTTCCA ATACATATGA AGAGGCAGCT
GCCTATATTC AATGCCAGTT TGAAGATCTG AACAGAAGAA AAGATACCAA GGAGATCTAT
ACTCACTTCA CCTGTGCCAC AGACACGAAG AATGTGCAGT TTGTTTTTGA TGCTGTTACA
GATGTCATCA TTAAAAACAA CTTAAAGGAA TGTGGACTTT ATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Evidence for dynein and astral microtubule-mediated cortical release and transport of Galphai/LGN/NuMA complex in mitotic cells
Mol. Biol. Cell 24 (7), 901-913 (2013)
Zheng Z, Wan Q, Liu J, Zhu H, Chu X and Du Q.


book

A human homeotic transformation resulting from mutations in PLCB4 and GNAI3 causes auriculocondylar syndrome
Am. J. Hum. Genet. 90 (5), 907-914 (2012)
Rieder MJ, Green GE, Park SS, Stamper BD, Gordon CT, Johnson JM, Cunniff CM, Smith JD, Emery SB, Lyonnet S, Amiel J, Holder M, Heggie AA, Bamshad MJ, Nickerson DA, Cox TC, Hing AV, Horst JA and Cunningham ML.


book

Two distinct aspects of coupling between Galpha(i) protein and G protein-activated K+ channel (GIRK) revealed by fluorescently labeled Galpha(i3) protein subunits
J. Biol. Chem. 286 (38), 33223-33235 (2011)
Berlin S, Tsemakhovich VA, Castel R, Ivanina T, Dessauer CW, Keren-Raifman T and Dascal N.


book

A GDI (AGS3) and a GEF (GIV) regulate autophagy by balancing G protein activity and growth factor signals
Mol. Biol. Cell 22 (5), 673-686 (2011)
Garcia-Marcos M, Ear J, Farquhar MG and Ghosh P.


book

Specific interaction of Galphai3 with the Oa1 G-protein coupled receptor controls the size and density of melanosomes in retinal pigment epithelium
PLoS ONE 6 (9), E24376 (2011)
Young A, Jiang M, Wang Y, Ahmedli NB, Ramirez J, Reese BE, Birnbaumer L and Farber DB.


book

Guanine nucleotide-binding regulatory proteins in retinal pigment epithelial cells
Proc. Natl. Acad. Sci. U.S.A. 88 (9), 3907-3911 (1991)
Jiang M, Pandey S, Tran VT and Fong HK.


book

Distinct guanine nucleotide binding and release properties of the three Gi proteins
J. Biol. Chem. 265 (11), 6268-6273 (1990)
Carty DJ, Padrell E, Codina J, Birnbaumer L, Hildebrandt JD and Iyengar R.


book

Presence of three distinct molecular species of Gi protein alpha subunit. Structure of rat cDNAs and human genomic DNAs
J. Biol. Chem. 263 (14), 6656-6664 (1988)
Itoh H, Toyama R, Kozasa T, Tsukamoto T, Matsuoka M and Kaziro Y.


book

Alpha i-3 cDNA encodes the alpha subunit of Gk, the stimulatory G protein of receptor-regulated K+ channels
J. Biol. Chem. 263 (14), 6746-6750 (1988)
Codina J, Olate J, Abramowitz J, Mattera R, Cook RG and Birnbaumer L.


book

The human genome encodes at least three non-allellic G proteins with alpha i-type subunits
FEBS Lett. 220 (1), 187-192 (1987)
Suki,W.N., Abramowitz,J., Mattera,R., Codina,J. and Birnbaumer,L.


 
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