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GNGT1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol GNGT1
Entrez Gene ID 2792
Full Name guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1
Synonyms GNG1
General protein information
Preferred Names
guanine nucleotide-binding protein G(T) subunit gamma-T1
Names
guanine nucleotide-binding protein G(T) subunit gamma-T1
transducin gamma chain
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

7

7q21.3

Summary Heterotrimeric guanine nucleotide-binding proteins (G proteins) transduce extracellular signals received by transmembrane receptors to effector proteins. Transducin is a guanine nucleotide-binding protein found specifically in rod outer segments, where it mediates activation by rhodopsin of a cyclic GTP-specific (guanosine monophosphate) phosphodiesterase. Transducin is also referred to as GMPase. GNGT1 encodes the gamma subunit of transducin (Hurley et al., 1984 [PubMed 6438626]; Scherer et al., 1996 [PubMed 8661128]).[supplied by OMIM, Mar 2008]. lac of sum
Disorder MIM:

189970


mRNA and Protein(s)

mRNA Protein Name
NM_021955 NP_068774 guanine nucleotide-binding protein G(T) subunit gamma-T1 precursor



Homo sapiens (human) GNGT1 NP_068774.1
Pan troglodytes (chimpanzee) GNGT1 XP_001168330.1
Macaca mulatta (Rhesus monkey) GNGT1 XP_001098610.2
Canis lupus familiaris (dog) GNGT1 NP_001003225.1
Bos taurus (cattle) GNGT1 NP_776752.1
Mus musculus (house mouse) Gngt1 NP_034444.1
Rattus norvegicus (Norway rat) Gngt1 NP_001129249.1
Danio rerio (zebrafish) gngt1 NP_956261.1
Xenopus (Silurana) tropicalis (western clawed frog) gngt1 NP_001165112.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GNGT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNGT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu04853 NM_021955 Homo sapiens guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1 (GNGT1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu04853
Clone ID Related Accession (Same CDS sequence) NM_021955
Accession Version NM_021955.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 225bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product guanine nucleotide-binding protein G(T) subunit gamma-T1 precursor
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC029367.1 and AC002076.2. On Sep 9, 2005 this sequence version replaced gi:41584205. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG435040.1, EL946418.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398, SAMEA2153307 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
ATGCCAGTAA TCAATATTGA GGACCTGACA GAAAAGGACA AATTGAAGAT GGAAGTTGAC 
CAGCTCAAGA AAGAAGTGAC ACTGGAAAGA ATGCTAGTTT CCAAATGTTG TGAAGAAGTA
AGAGATTACG TTGAAGAACG ATCTGGCGAG GATCCACTGG TAAAGGGCAT CCCAGAGGAC
AAAAATCCCT TCAAGGAGCT CAAAGGAGGC TGTGTGATTT CATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_068774.1
CDS149..373
Misc Feature(1)110..112(+)
Misc Feature(2)182..352(+)
Misc Feature(3)182..340(+)
Exon (1)1..137
Gene:GNGT1
Gene Synonym:
Exon (2)138..244
Gene:GNGT1
Gene Synonym:
Exon (3)245..628
Gene:GNGT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_021955
Organism Homo sapiens (human)
Definition Homo sapiens guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1 (GNGT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_021955

ORF Insert Sequence:

1
61
121
181
ATGCCAGTAA TCAATATTGA GGACCTGACA GAAAAGGACA AATTGAAGAT GGAAGTTGAC 
CAGCTCAAGA AAGAAGTGAC ACTGGAAAGA ATGCTAGTTT CCAAATGTTG TGAAGAAGTA
AGAGATTACG TTGAAGAACG ATCTGGCGAG GATCCACTGG TAAAGGGCAT CCCAGAGGAC
AAAAATCCCT TCAAGGAGCT CAAAGGAGGC TGTGTGATTT CATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

IGF-1 activates a cilium-localized noncanonical Gbetagamma signaling pathway that regulates cell-cycle progression
Dev. Cell 26 (4), 358-368 (2013)
Yeh C, Li A, Chuang JZ, Saito M, Caceres A and Sung CH.


book

Genome-wide association study of lung function decline in adults with and without asthma
J. Allergy Clin. Immunol. 129 (5), 1218-1228 (2012)
Imboden M, Bouzigon E, Curjuric I, Ramasamy A, Kumar A, Hancock DB, Wilk JB, Vonk JM, Thun GA, Siroux V, Nadif R, Monier F, Gonzalez JR, Wjst M, Heinrich J, Loehr LR, Franceschini N, North KE, Altmuller J, Koppelman GH, Guerra S, Kronenberg F, Lathrop M, Moffatt MF, O'Connor GT, Strachan DP, Postma DS, London SJ, Schindler C, Kogevinas M, Kauffmann F, Jarvis DL, Demenais F and Probst-Hensch NM.


book

The WD40 repeat protein WDR26 binds Gbetagamma and promotes Gbetagamma-dependent signal transduction and leukocyte migration
J. Biol. Chem. 286 (51), 43902-43912 (2011)
Sun Z, Tang X, Lin F and Chen S.


book

G protein betagamma subunits regulate cell adhesion through Rap1a and its effector Radil
J. Biol. Chem. 285 (9), 6538-6551 (2010)
Ahmed SM, Daulat AM, Meunier A and Angers S.


book

G Protein betagamma subunits augment UVB-induced apoptosis by stimulating the release of soluble heparin-binding epidermal growth factor from human keratinocytes
J. Biol. Chem. 282 (34), 24720-24730 (2007)
Seo M, Lee MJ, Heo JH, Lee YI, Kim Y, Kim SY, Lee ES and Juhnn YS.


book

Differential ability to form the G protein betagamma complex among members of the beta and gamma subunit families
J. Biol. Chem. 271 (12), 7141-7146 (1996)
Yan K, Kalyanaraman V and Gautam N.


book

Isolation of cDNA clones encoding eight different human G protein gamma subunits, including three novel forms designated the gamma 4, gamma 10, and gamma 11 subunits
J. Biol. Chem. 270 (37), 21765-21771 (1995)
Ray K, Kunsch C, Bonner LM and Robishaw JD.


book

Structure of the bovine transducin gamma subunit gene and analysis of promoter function in transgenic mice
Exp. Eye Res. 56 (4), 497-507 (1993)
Tao L, Pandey S, Simon MI and Fong HK.


book

G-protein beta gamma dimers. Membrane targeting requires subunit coexpression and intact gamma C-A-A-X domain
J. Biol. Chem. 266 (9), 5363-5366 (1991)
Simonds WF, Butrynski JE, Gautam N, Unson CG and Spiegel AM.


book

Isolation and characterization of a cDNA clone for the gamma subunit of bovine retinal transducin
Proc. Natl. Acad. Sci. U.S.A. 81 (22), 6948-6952 (1984)
Hurley,J.B., Fong,H.K., Teplow,D.B., Dreyer,W.J. and Simon,M.I.


 
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