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RBM20 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol RBM20
Entrez Gene ID 282996
Full Name RNA binding motif protein 20
General protein information
Preferred Names

RNA binding motif protein 20

Names

RNA-binding protein 20
probable RNA-binding protein 20
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

10

10q25.2
Summary This gene encodes a protein that binds RNA and regulates splicing. Mutations in this gene have been associated with familial dilated cardiomyopathy. [provided by RefSeq, Apr 2014].
Disorder MIM:

613171

Disorder Html: Cardiomyopathy, dilated, 1DD, 613172 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_017016104.1 XP_016871593.1 RNA-binding protein 20 isoform X2
XM_017016103.2 XP_016871592.1 RNA-binding protein 20 isoform X1
XM_017016103.1 XP_016871592.1 RNA-binding protein 20 isoform X1
NM_001134363.2 NP_001127835.2 RNA-binding protein 20
NM_001134363.3 NP_001127835.2 RNA-binding protein 20
XM_011539697.2 XP_011537999.1 RNA-binding protein 20 isoform X2
XM_017016104.2 XP_016871593.1 RNA-binding protein 20 isoform X2

Pathways from BioSystems


Homologies


Homo sapiens (human) RBM20 NP_001127835.1
Pan troglodytes (chimpanzee) RBM20 XP_508032.3
Canis lupus familiaris (dog) RBM20 XP_544017.3
Gallus gallus (chicken) RBM20 XP_421755.4
Bos taurus (cattle) RBM20 NP_001179542.1
Mus musculus (house mouse) Rbm20 NP_001164318.1
Xenopus tropicalis (tropical clawed frog) rbm20 XP_002942947.2
RBM20 XP_001087170.2
Rattus norvegicus (Norway rat) Rbm20 NP_001101081.2
Danio rerio (zebrafish) LOC555579 XP_683222.5

Gene Ontology


Bibliography

Related articles in PubMed

RBM20 Regulates Circular RNA Production From the Titin Gene.
Khan MA, Reckman YJ, Aufiero S, van den Hoogenhof MM, van der Made I, Beqqali A, Koolbergen DR, Rasmussen TB, van der Velden J, Creemers EE, Pinto YM
Circulation research119(9)996-1003(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RBM20 is crucial for the formation of a subset of circular RNAs that originate from the I-band of the titin gene.
Title: RBM20 Regulates Circular RNA Production From the Titin Gene.

Heterozygous loss of RBM20 suffices to profoundly impair myocyte biomechanics by its disturbance of TTN splicing causing dilated cardiomyopathy.
Title: A mutation in the glutamate-rich region of RNA-binding motif protein 20 causes dilated cardiomyopathy through missplicing of titin and impaired Frank-Starling mechanism.

Mutations in RBM20 cause dilated cardiomyopathy along with dysregulated isoform switch.
Title: Emerging Role for RBM20 and its Splicing Substrates in Cardiac Function and Heart Failure.

RBM20 familial dilated cardiomyopathy is a developmental disorder initiated by molecular defects that pattern maladaptive cellular mechanisms of pathological cardiac remodeling.
Title: Modeling structural and functional deficiencies of RBM20 familial dilated cardiomyopathy using human induced pluripotent stem cells.

In failing hearts, reduced expression of RBM20 affected alternative splicing of several direct targets, indicating that differences in RBM20 expression may affect cardiac function.
Title: RNA-binding protein RBM20 represses splicing to orchestrate cardiac pre-mRNA processing.

The following RBM20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RBM20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu23596 NM_001134363.3
Latest version!		 Homo sapiens RNA binding motif protein 20 (RBM20), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $459.50-$643.30
$919.00
OHu78531 XM_017016104.2
Latest version!		 Homo sapiens RNA binding motif protein 20 (RBM20), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $559.30
$799.00
OHu78531 XM_011539697.2
Latest version!		 Homo sapiens RNA binding motif protein 20 (RBM20), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $559.30
$799.00
OHu91314 XM_017016103.2
Latest version!		 Homo sapiens RNA binding motif protein 20 (RBM20), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
View Old Accession Versions >>
OHu91314 XM_017016103.1 Homo sapiens RNA binding motif protein 20 (RBM20), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 19-21 $643.30
$919.00
OHu23596 NM_001134363.2 Homo sapiens RNA binding motif protein 20 (RBM20), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $459.50-$643.30
$919.00
OHu78531 XM_017016104.1 Homo sapiens RNA binding motif protein 20 (RBM20), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 16 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu23596
Clone ID Related Accession (Same CDS sequence) NM_001134363.2 , NM_001134363.3
Accession Version NM_001134363.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3684bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-05
Organism Homo sapiens(human)
Product RNA-binding protein 20
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU822950.1, KF459660.1 and CB044334.1. On Apr 15, 2014 this sequence version replaced NM_001134363.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU822950.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGTGCTGG CAGCAGCCAT GAGCCAGGAC GCGGACCCCA GCGGTCCGGA GCAGCCGGAC 
AGAGTTGCCT GCAGTGTGCC TGGTGCCCGG GCGTCCCCGG CACCCTCCGG CCCGCGAGGG 
ATGCAGCAGC CGCCGCCGCC GCCCCAGCCA CCGCCCCCGC CCCAAGCCGG CCTACCCCAG 
ATCATCCAAA ATGCCGCCAA GCTCCTGGAC AAGAACCCAT TCTCGGTCAG TAACCCGAAC 
CCTCTGCTTC CTTCACCTGC CAGTCTCCAG CTGGCTCAAC TGCAGGCCCA GCTCACCCTC 
CACCGGCTGA AGCTGGCACA GACAGCTGTC ACCAACAACA CTGCAGCCGC CACAGTCCTG 
AACCAAGTCC TCTCCAAAGT GGCCATGTCC CAGCCTCTCT TCAATCAACT GAGGCATCCG 
TCTGTGATCA CTGGCCCCCA CGGCCATGCT GGGGTTCCCC AACATGCTGC AGCCATACCC 
AGTACCCGGT TTCCCTCTAA TGCAATTGCC TTTTCACCCC CCAGCCAGAC ACGAGGCCCC 
GGACCCTCCA TGAACCTTCC CAACCAGCCA CCCAGTGCCA TGGTGATGCA TCCTTTCACT 
GGGGTAATGC CTCAGACCCC TGGCCAGCCA GCAGTCATCT TGGGCATTGG CAAGACTGGG 
CCTGCTCCAG CTACAGCAGG ATTCTATGAG TATGGCAAAG CCAGCTCTGG CCAGACATAT 
GGCCCTGAAA CAGATGGTCA GCCTGGCTTC CTGCCATCCT CGGCCTCAAC CTCGGGCAGT 
GTGACCTATG AAGGGCACTA CAGCCACACA GGGCAGGATG GTCAAGCTGC CTTTTCCAAA 
GATTTTTACG GACCCAACTC CCAAGGTTCA CATGTGGCCA GCGGATTTCC AGCTGAGCAG 
GCTGGGGGCC TGAAAAGTGA GGTCGGGCCA CTGCTGCAGG GCACAAACAG CCAATGGGAG 
AGCCCCCATG GATTCTCGGG CCAAAGCAAG CCTGATCTCA CAGCAGGTCC CATGTGGCCT 
CCACCCCACA ACCAGCCCTA TGAGCTGTAC GACCCCGAGG AACCAACCTC AGACAGGACA 
CCTCCTTCCT TCGGGGGTCG GCTTAACAAC AGCAAACAGG GTTTTATCGG TGCTGGGCGG 
AGGGCCAAGG AGGACCAGGC GTTGCTATCT GTGCGGCCCC TGCAGGCTCA TGAGCTGAAC 
GACTTTCACG GTGTGGCCCC CCTCCACTTG CCGCATATCT GTAGCATCTG TGACAAGAAG 
GTGTTTGATT TGAAGGACTG GGAGCTGCAT GTGAAAGGGA AGCTGCACGC TCAGAAATGC 
CTGGTCTTCT CTGAAAATGC TGGCATCCGG TGTATACTTG GTTCGGCAGA GGGAACATTG 
TGTGCTTCTC CCAACAGCAC AGCTGTTTAT AACCCTGCTG GGAATGAAGA TTATGCCTCA 
AATCTTGGAA CATCATACGT GCCCATTCCA GCAAGGTCAT TCACTCAGTC AAGCCCCACA 
TTTCCTTTGG CTTCTGTGGG GACAACTTTT GCACAGCGGA AAGGGGCTGG CCGTGTGGTG 
CACATCTGCA ATCTCCCTGA AGGAAGCTGC ACTGAGAATG ACGTCATTAA CCTGGGGCTG 
CCCTTTGGAA AGGTCACTAA TTACATCCTC ATGAAGTCGA CTAATCAGGC CTTTTTAGAG 
ATGGCTTACA CAGAAGCTGC ACAGGCCATG GTCCAGTATT ATCAAGAAAA ATCTGCTGTG 
ATCAATGGTG AGAAGTTGCT CATTCGGATG TCCAAGAGAT ACAAGGAATT GCAGCTCAAG 
AAACCCGGGA AGGCCGTGGC TGCCATCATC CAGGACATCC ATTCCCAGAG GGAGAGGGAC 
ATGTTCCGGG AAGCAGACAG ATATGGCCCA GAAAGGCCGC GGTCTCGTAG TCCGGTGAGC 
CGGTCACTCT CCCCGAGGTC CCACACTCCC AGCTTCACCT CCTGCAGCTC TTCCCACAGC 
CCTCCGGGCC CCTCCCGGGC TGACTGGGGC AATGGCCGGG ACTCCTGGGA GCACTCTCCC 
TATGCCAGGA GGGAGGAAGA GCGAGACCCG GCTCCCTGGA GGGACAACGG AGATGACAAG 
AGGGACAGGA TGGACCCCTG GGCACATGAT CGCAAACACC ACCCCCGGCA ACTGGACAAG 
GCTGAGTTGG ACGAGCGACC AGAAGGAGGG AGGCCCCACC GGGAGAAGTA CCCGAGATCT 
GGGTCTCCCA ACCTGCCCCA CTCTGTGTCC AGCTACAAAA GCCGTGAAGA CGGCTACTAC 
CGGAAAGAGC CCAAAGCCAA GTCGGACAAG TATCTGAAGC AGCAGCAGGA TGCCCCCGGG 
AGGTCCAGGA GGAAAGACGA GGCCAGGCTG CGGGAAAGCA GACACCCCCA TCCGGATGAC 
TCAGGCAAGG AAGATGGGCT GGGGCCAAAG GTCACTAGGG CCCCTGAGGG CGCCAAGGCC 
AAGCAGAATG AGAAAAATAA AACCAAGAGA ACTGATAGAG ACCAAGAAGG AGCTGATGAT 
AGAAAAGAAA ACACAATGGC AGAGAATGAG GCTGGAAAAG AGGAACAGGA GGGCATGGAA 
GAAAGCCCTC AATCAGTGGG CAGACAGGAG AAAGAAGCAG AGTTCTCTGA TCCGGAAAAC 
ACAAGGACAA AGAAGGAACA AGATTGGGAG AGTGAAAGTG AGGCAGAGGG GGAGAGCTGG 
TATCCCACTA ACATGGAGGA GCTGGTGACA GTGGACGAGG TTGGGGAAGA AGAAGATTTT 
ATCGTGGAAC CAGACATCCC AGAGCTGGAA GAAATTGTGC CCATTGACCA GAAAGACAAA 
ATTTGCCCAG AAACATGTCT GTGTGTGACA ACCACCTTAG ACTTAGACCT GGCCCAGGAT 
TTCCCCAAGG AAGGAGTCAA GGCCGTAGGG AATGGGGCTG CAGAAATCAG CCTCAAGTCA 
CCCAGAGAAC TGCCCTCTGC TTCCACAAGC TGTCCCAGTG ACATGGACGT GGAAATGCCT 
GGCCTAAATC TGGATGCTGA GCGGAAGCCA GCTGAAAGTG AGACAGGCCT CTCCCTGGAG 
GATTCAGATT GCTACGAGAA GGAGGCAAAG GGAGTGGAGA GCTCAGATGT TCATCCAGCC 
CCTACAGTCC AGCAAATGTC TTCCCCTAAG CCAGCAGAGG AGAGGGCCCG GCAGCCAAGC 
CCATTTGTGG ATGATTGCAA GACCAGGGGG ACCCCCGAAG ATGGGGCTTG TGAAGGCAGC 
CCCCTGGAGG AGAAAGCCAG CCCCCCCATC GAAACTGACC TCCAAAACCA AGCTTGCCAA 
GAAGTGTTGA CCCCGGAAAA CTCCAGGTAC GTGGAAATGA AATCTCTGGA GGTGAGGTCA 
CCAGAGTACA CTGAAGTGGA ACTGAAACAG CCCCTTTCTT TGCCCTCTTG GGAACCAGAG 
GATGTGTTCA GTGAACTTAG CATTCCTCTA GGGGTGGAGT TCGTGGTTCC CAGGACTGGC 
TTTTATTGCA AGCTGTGTGG GCTGTTCTAC ACGAGCGAGG AGACAGCAAA GATGAGCCAC 
TGCCGCAGCG CTGTCCACTA CAGGAACTTA CAGAAATATT TGTCCCAGCT GGCCGAGGAG 
GGCCTCAAGG AGACCGAGGG GGCAGATAGC CCGAGGCCAG AGGACAGCGG AATCGTGCCA 
CGCTTCGAAA GGAAAAAGCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001127835.2
CDS59..3742
Translation

Target ORF information:

RefSeq Version NM_001134363.2
Organism Homo sapiens(human)
Definition Homo sapiens RNA binding motif protein 20 (RBM20), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001134363.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGTGCTGG CAGCAGCCAT GAGCCAGGAC GCGGACCCCA GCGGTCCGGA GCAGCCGGAC 
AGAGTTGCCT GCAGTGTGCC TGGTGCCCGG GCGTCCCCGG CACCCTCCGG CCCGCGAGGG 
ATGCAGCAGC CGCCGCCGCC GCCCCAGCCA CCGCCCCCGC CCCAAGCCGG CCTACCCCAG 
ATCATCCAAA ATGCCGCCAA GCTCCTGGAC AAGAACCCAT TCTCGGTCAG TAACCCGAAC 
CCTCTGCTTC CTTCACCTGC CAGTCTCCAG CTGGCTCAAC TGCAGGCCCA GCTCACCCTC 
CACCGGCTGA AGCTGGCACA GACAGCTGTC ACCAACAACA CTGCAGCCGC CACAGTCCTG 
AACCAAGTCC TCTCCAAAGT GGCCATGTCC CAGCCTCTCT TCAATCAACT GAGGCATCCG 
TCTGTGATCA CTGGCCCCCA CGGCCATGCT GGGGTTCCCC AACATGCTGC AGCCATACCC 
AGTACCCGGT TTCCCTCTAA TGCAATTGCC TTTTCACCCC CCAGCCAGAC ACGAGGCCCC 
GGACCCTCCA TGAACCTTCC CAACCAGCCA CCCAGTGCCA TGGTGATGCA TCCTTTCACT 
GGGGTAATGC CTCAGACCCC TGGCCAGCCA GCAGTCATCT TGGGCATTGG CAAGACTGGG 
CCTGCTCCAG CTACAGCAGG ATTCTATGAG TATGGCAAAG CCAGCTCTGG CCAGACATAT 
GGCCCTGAAA CAGATGGTCA GCCTGGCTTC CTGCCATCCT CGGCCTCAAC CTCGGGCAGT 
GTGACCTATG AAGGGCACTA CAGCCACACA GGGCAGGATG GTCAAGCTGC CTTTTCCAAA 
GATTTTTACG GACCCAACTC CCAAGGTTCA CATGTGGCCA GCGGATTTCC AGCTGAGCAG 
GCTGGGGGCC TGAAAAGTGA GGTCGGGCCA CTGCTGCAGG GCACAAACAG CCAATGGGAG 
AGCCCCCATG GATTCTCGGG CCAAAGCAAG CCTGATCTCA CAGCAGGTCC CATGTGGCCT 
CCACCCCACA ACCAGCCCTA TGAGCTGTAC GACCCCGAGG AACCAACCTC AGACAGGACA 
CCTCCTTCCT TCGGGGGTCG GCTTAACAAC AGCAAACAGG GTTTTATCGG TGCTGGGCGG 
AGGGCCAAGG AGGACCAGGC GTTGCTATCT GTGCGGCCCC TGCAGGCTCA TGAGCTGAAC 
GACTTTCACG GTGTGGCCCC CCTCCACTTG CCGCATATCT GTAGCATCTG TGACAAGAAG 
GTGTTTGATT TGAAGGACTG GGAGCTGCAT GTGAAAGGGA AGCTGCACGC TCAGAAATGC 
CTGGTCTTCT CTGAAAATGC TGGCATCCGG TGTATACTTG GTTCGGCAGA GGGAACATTG 
TGTGCTTCTC CCAACAGCAC AGCTGTTTAT AACCCTGCTG GGAATGAAGA TTATGCCTCA 
AATCTTGGAA CATCATACGT GCCCATTCCA GCAAGGTCAT TCACTCAGTC AAGCCCCACA 
TTTCCTTTGG CTTCTGTGGG GACAACTTTT GCACAGCGGA AAGGGGCTGG CCGTGTGGTG 
CACATCTGCA ATCTCCCTGA AGGAAGCTGC ACTGAGAATG ACGTCATTAA CCTGGGGCTG 
CCCTTTGGAA AGGTCACTAA TTACATCCTC ATGAAGTCGA CTAATCAGGC CTTTTTAGAG 
ATGGCTTACA CAGAAGCTGC ACAGGCCATG GTCCAGTATT ATCAAGAAAA ATCTGCTGTG 
ATCAATGGTG AGAAGTTGCT CATTCGGATG TCCAAGAGAT ACAAGGAATT GCAGCTCAAG 
AAACCCGGGA AGGCCGTGGC TGCCATCATC CAGGACATCC ATTCCCAGAG GGAGAGGGAC 
ATGTTCCGGG AAGCAGACAG ATATGGCCCA GAAAGGCCGC GGTCTCGTAG TCCGGTGAGC 
CGGTCACTCT CCCCGAGGTC CCACACTCCC AGCTTCACCT CCTGCAGCTC TTCCCACAGC 
CCTCCGGGCC CCTCCCGGGC TGACTGGGGC AATGGCCGGG ACTCCTGGGA GCACTCTCCC 
TATGCCAGGA GGGAGGAAGA GCGAGACCCG GCTCCCTGGA GGGACAACGG AGATGACAAG 
AGGGACAGGA TGGACCCCTG GGCACATGAT CGCAAACACC ACCCCCGGCA ACTGGACAAG 
GCTGAGTTGG ACGAGCGACC AGAAGGAGGG AGGCCCCACC GGGAGAAGTA CCCGAGATCT 
GGGTCTCCCA ACCTGCCCCA CTCTGTGTCC AGCTACAAAA GCCGTGAAGA CGGCTACTAC 
CGGAAAGAGC CCAAAGCCAA GTCGGACAAG TATCTGAAGC AGCAGCAGGA TGCCCCCGGG 
AGGTCCAGGA GGAAAGACGA GGCCAGGCTG CGGGAAAGCA GACACCCCCA TCCGGATGAC 
TCAGGCAAGG AAGATGGGCT GGGGCCAAAG GTCACTAGGG CCCCTGAGGG CGCCAAGGCC 
AAGCAGAATG AGAAAAATAA AACCAAGAGA ACTGATAGAG ACCAAGAAGG AGCTGATGAT 
AGAAAAGAAA ACACAATGGC AGAGAATGAG GCTGGAAAAG AGGAACAGGA GGGCATGGAA 
GAAAGCCCTC AATCAGTGGG CAGACAGGAG AAAGAAGCAG AGTTCTCTGA TCCGGAAAAC 
ACAAGGACAA AGAAGGAACA AGATTGGGAG AGTGAAAGTG AGGCAGAGGG GGAGAGCTGG 
TATCCCACTA ACATGGAGGA GCTGGTGACA GTGGACGAGG TTGGGGAAGA AGAAGATTTT 
ATCGTGGAAC CAGACATCCC AGAGCTGGAA GAAATTGTGC CCATTGACCA GAAAGACAAA 
ATTTGCCCAG AAACATGTCT GTGTGTGACA ACCACCTTAG ACTTAGACCT GGCCCAGGAT 
TTCCCCAAGG AAGGAGTCAA GGCCGTAGGG AATGGGGCTG CAGAAATCAG CCTCAAGTCA 
CCCAGAGAAC TGCCCTCTGC TTCCACAAGC TGTCCCAGTG ACATGGACGT GGAAATGCCT 
GGCCTAAATC TGGATGCTGA GCGGAAGCCA GCTGAAAGTG AGACAGGCCT CTCCCTGGAG 
GATTCAGATT GCTACGAGAA GGAGGCAAAG GGAGTGGAGA GCTCAGATGT TCATCCAGCC 
CCTACAGTCC AGCAAATGTC TTCCCCTAAG CCAGCAGAGG AGAGGGCCCG GCAGCCAAGC 
CCATTTGTGG ATGATTGCAA GACCAGGGGG ACCCCCGAAG ATGGGGCTTG TGAAGGCAGC 
CCCCTGGAGG AGAAAGCCAG CCCCCCCATC GAAACTGACC TCCAAAACCA AGCTTGCCAA 
GAAGTGTTGA CCCCGGAAAA CTCCAGGTAC GTGGAAATGA AATCTCTGGA GGTGAGGTCA 
CCAGAGTACA CTGAAGTGGA ACTGAAACAG CCCCTTTCTT TGCCCTCTTG GGAACCAGAG 
GATGTGTTCA GTGAACTTAG CATTCCTCTA GGGGTGGAGT TCGTGGTTCC CAGGACTGGC 
TTTTATTGCA AGCTGTGTGG GCTGTTCTAC ACGAGCGAGG AGACAGCAAA GATGAGCCAC 
TGCCGCAGCG CTGTCCACTA CAGGAACTTA CAGAAATATT TGTCCCAGCT GGCCGAGGAG 
GGCCTCAAGG AGACCGAGGG GGCAGATAGC CCGAGGCCAG AGGACAGCGG AATCGTGCCA 
CGCTTCGAAA GGAAAAAGCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu23596
Clone ID Related Accession (Same CDS sequence) NM_001134363.2 , NM_001134363.3
Accession Version NM_001134363.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3684bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-13
Organism Homo sapiens(human)
Product RNA-binding protein 20
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL359260.20, EU822950.1 and KF459660.1. On Nov 23, 2018 this sequence version replaced NM_001134363.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU822950.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000369519.4/ ENSP00000358532.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGTGCTGG CAGCAGCCAT GAGCCAGGAC GCGGACCCCA GCGGTCCGGA GCAGCCGGAC 
AGAGTTGCCT GCAGTGTGCC TGGTGCCCGG GCGTCCCCGG CACCCTCCGG CCCGCGAGGG 
ATGCAGCAGC CGCCGCCGCC GCCCCAGCCA CCGCCCCCGC CCCAAGCCGG CCTACCCCAG 
ATCATCCAAA ATGCCGCCAA GCTCCTGGAC AAGAACCCAT TCTCGGTCAG TAACCCGAAC 
CCTCTGCTTC CTTCACCTGC CAGTCTCCAG CTGGCTCAAC TGCAGGCCCA GCTCACCCTC 
CACCGGCTGA AGCTGGCACA GACAGCTGTC ACCAACAACA CTGCAGCCGC CACAGTCCTG 
AACCAAGTCC TCTCCAAAGT GGCCATGTCC CAGCCTCTCT TCAATCAACT GAGGCATCCG 
TCTGTGATCA CTGGCCCCCA CGGCCATGCT GGGGTTCCCC AACATGCTGC AGCCATACCC 
AGTACCCGGT TTCCCTCTAA TGCAATTGCC TTTTCACCCC CCAGCCAGAC ACGAGGCCCC 
GGACCCTCCA TGAACCTTCC CAACCAGCCA CCCAGTGCCA TGGTGATGCA TCCTTTCACT 
GGGGTAATGC CTCAGACCCC TGGCCAGCCA GCAGTCATCT TGGGCATTGG CAAGACTGGG 
CCTGCTCCAG CTACAGCAGG ATTCTATGAG TATGGCAAAG CCAGCTCTGG CCAGACATAT 
GGCCCTGAAA CAGATGGTCA GCCTGGCTTC CTGCCATCCT CGGCCTCAAC CTCGGGCAGT 
GTGACCTATG AAGGGCACTA CAGCCACACA GGGCAGGATG GTCAAGCTGC CTTTTCCAAA 
GATTTTTACG GACCCAACTC CCAAGGTTCA CATGTGGCCA GCGGATTTCC AGCTGAGCAG 
GCTGGGGGCC TGAAAAGTGA GGTCGGGCCA CTGCTGCAGG GCACAAACAG CCAATGGGAG 
AGCCCCCATG GATTCTCGGG CCAAAGCAAG CCTGATCTCA CAGCAGGTCC CATGTGGCCT 
CCACCCCACA ACCAGCCCTA TGAGCTGTAC GACCCCGAGG AACCAACCTC AGACAGGACA 
CCTCCTTCCT TCGGGGGTCG GCTTAACAAC AGCAAACAGG GTTTTATCGG TGCTGGGCGG 
AGGGCCAAGG AGGACCAGGC GTTGCTATCT GTGCGGCCCC TGCAGGCTCA TGAGCTGAAC 
GACTTTCACG GTGTGGCCCC CCTCCACTTG CCGCATATCT GTAGCATCTG TGACAAGAAG 
GTGTTTGATT TGAAGGACTG GGAGCTGCAT GTGAAAGGGA AGCTGCACGC TCAGAAATGC 
CTGGTCTTCT CTGAAAATGC TGGCATCCGG TGTATACTTG GTTCGGCAGA GGGAACATTG 
TGTGCTTCTC CCAACAGCAC AGCTGTTTAT AACCCTGCTG GGAATGAAGA TTATGCCTCA 
AATCTTGGAA CATCATACGT GCCCATTCCA GCAAGGTCAT TCACTCAGTC AAGCCCCACA 
TTTCCTTTGG CTTCTGTGGG GACAACTTTT GCACAGCGGA AAGGGGCTGG CCGTGTGGTG 
CACATCTGCA ATCTCCCTGA AGGAAGCTGC ACTGAGAATG ACGTCATTAA CCTGGGGCTG 
CCCTTTGGAA AGGTCACTAA TTACATCCTC ATGAAGTCGA CTAATCAGGC CTTTTTAGAG 
ATGGCTTACA CAGAAGCTGC ACAGGCCATG GTCCAGTATT ATCAAGAAAA ATCTGCTGTG 
ATCAATGGTG AGAAGTTGCT CATTCGGATG TCCAAGAGAT ACAAGGAATT GCAGCTCAAG 
AAACCCGGGA AGGCCGTGGC TGCCATCATC CAGGACATCC ATTCCCAGAG GGAGAGGGAC 
ATGTTCCGGG AAGCAGACAG ATATGGCCCA GAAAGGCCGC GGTCTCGTAG TCCGGTGAGC 
CGGTCACTCT CCCCGAGGTC CCACACTCCC AGCTTCACCT CCTGCAGCTC TTCCCACAGC 
CCTCCGGGCC CCTCCCGGGC TGACTGGGGC AATGGCCGGG ACTCCTGGGA GCACTCTCCC 
TATGCCAGGA GGGAGGAAGA GCGAGACCCG GCTCCCTGGA GGGACAACGG AGATGACAAG 
AGGGACAGGA TGGACCCCTG GGCACATGAT CGCAAACACC ACCCCCGGCA ACTGGACAAG 
GCTGAGTTGG ACGAGCGACC AGAAGGAGGG AGGCCCCACC GGGAGAAGTA CCCGAGATCT 
GGGTCTCCCA ACCTGCCCCA CTCTGTGTCC AGCTACAAAA GCCGTGAAGA CGGCTACTAC 
CGGAAAGAGC CCAAAGCCAA GTCGGACAAG TATCTGAAGC AGCAGCAGGA TGCCCCCGGG 
AGGTCCAGGA GGAAAGACGA GGCCAGGCTG CGGGAAAGCA GACACCCCCA TCCGGATGAC 
TCAGGCAAGG AAGATGGGCT GGGGCCAAAG GTCACTAGGG CCCCTGAGGG CGCCAAGGCC 
AAGCAGAATG AGAAAAATAA AACCAAGAGA ACTGATAGAG ACCAAGAAGG AGCTGATGAT 
AGAAAAGAAA ACACAATGGC AGAGAATGAG GCTGGAAAAG AGGAACAGGA GGGCATGGAA 
GAAAGCCCTC AATCAGTGGG CAGACAGGAG AAAGAAGCAG AGTTCTCTGA TCCGGAAAAC 
ACAAGGACAA AGAAGGAACA AGATTGGGAG AGTGAAAGTG AGGCAGAGGG GGAGAGCTGG 
TATCCCACTA ACATGGAGGA GCTGGTGACA GTGGACGAGG TTGGGGAAGA AGAAGATTTT 
ATCGTGGAAC CAGACATCCC AGAGCTGGAA GAAATTGTGC CCATTGACCA GAAAGACAAA 
ATTTGCCCAG AAACATGTCT GTGTGTGACA ACCACCTTAG ACTTAGACCT GGCCCAGGAT 
TTCCCCAAGG AAGGAGTCAA GGCCGTAGGG AATGGGGCTG CAGAAATCAG CCTCAAGTCA 
CCCAGAGAAC TGCCCTCTGC TTCCACAAGC TGTCCCAGTG ACATGGACGT GGAAATGCCT 
GGCCTAAATC TGGATGCTGA GCGGAAGCCA GCTGAAAGTG AGACAGGCCT CTCCCTGGAG 
GATTCAGATT GCTACGAGAA GGAGGCAAAG GGAGTGGAGA GCTCAGATGT TCATCCAGCC 
CCTACAGTCC AGCAAATGTC TTCCCCTAAG CCAGCAGAGG AGAGGGCCCG GCAGCCAAGC 
CCATTTGTGG ATGATTGCAA GACCAGGGGG ACCCCCGAAG ATGGGGCTTG TGAAGGCAGC 
CCCCTGGAGG AGAAAGCCAG CCCCCCCATC GAAACTGACC TCCAAAACCA AGCTTGCCAA 
GAAGTGTTGA CCCCGGAAAA CTCCAGGTAC GTGGAAATGA AATCTCTGGA GGTGAGGTCA 
CCAGAGTACA CTGAAGTGGA ACTGAAACAG CCCCTTTCTT TGCCCTCTTG GGAACCAGAG 
GATGTGTTCA GTGAACTTAG CATTCCTCTA GGGGTGGAGT TCGTGGTTCC CAGGACTGGC 
TTTTATTGCA AGCTGTGTGG GCTGTTCTAC ACGAGCGAGG AGACAGCAAA GATGAGCCAC 
TGCCGCAGCG CTGTCCACTA CAGGAACTTA CAGAAATATT TGTCCCAGCT GGCCGAGGAG 
GGCCTCAAGG AGACCGAGGG GGCAGATAGC CCGAGGCCAG AGGACAGCGG AATCGTGCCA 
CGCTTCGAAA GGAAAAAGCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001127835.2
CDS120..3803
Translation

Target ORF information:

RefSeq Version NM_001134363.3
Organism Homo sapiens(human)
Definition Homo sapiens RNA binding motif protein 20 (RBM20), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001134363.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
ATGGTGCTGG CAGCAGCCAT GAGCCAGGAC GCGGACCCCA GCGGTCCGGA GCAGCCGGAC 
AGAGTTGCCT GCAGTGTGCC TGGTGCCCGG GCGTCCCCGG CACCCTCCGG CCCGCGAGGG 
ATGCAGCAGC CGCCGCCGCC GCCCCAGCCA CCGCCCCCGC CCCAAGCCGG CCTACCCCAG 
ATCATCCAAA ATGCCGCCAA GCTCCTGGAC AAGAACCCAT TCTCGGTCAG TAACCCGAAC 
CCTCTGCTTC CTTCACCTGC CAGTCTCCAG CTGGCTCAAC TGCAGGCCCA GCTCACCCTC 
CACCGGCTGA AGCTGGCACA GACAGCTGTC ACCAACAACA CTGCAGCCGC CACAGTCCTG 
AACCAAGTCC TCTCCAAAGT GGCCATGTCC CAGCCTCTCT TCAATCAACT GAGGCATCCG 
TCTGTGATCA CTGGCCCCCA CGGCCATGCT GGGGTTCCCC AACATGCTGC AGCCATACCC 
AGTACCCGGT TTCCCTCTAA TGCAATTGCC TTTTCACCCC CCAGCCAGAC ACGAGGCCCC 
GGACCCTCCA TGAACCTTCC CAACCAGCCA CCCAGTGCCA TGGTGATGCA TCCTTTCACT 
GGGGTAATGC CTCAGACCCC TGGCCAGCCA GCAGTCATCT TGGGCATTGG CAAGACTGGG 
CCTGCTCCAG CTACAGCAGG ATTCTATGAG TATGGCAAAG CCAGCTCTGG CCAGACATAT 
GGCCCTGAAA CAGATGGTCA GCCTGGCTTC CTGCCATCCT CGGCCTCAAC CTCGGGCAGT 
GTGACCTATG AAGGGCACTA CAGCCACACA GGGCAGGATG GTCAAGCTGC CTTTTCCAAA 
GATTTTTACG GACCCAACTC CCAAGGTTCA CATGTGGCCA GCGGATTTCC AGCTGAGCAG 
GCTGGGGGCC TGAAAAGTGA GGTCGGGCCA CTGCTGCAGG GCACAAACAG CCAATGGGAG 
AGCCCCCATG GATTCTCGGG CCAAAGCAAG CCTGATCTCA CAGCAGGTCC CATGTGGCCT 
CCACCCCACA ACCAGCCCTA TGAGCTGTAC GACCCCGAGG AACCAACCTC AGACAGGACA 
CCTCCTTCCT TCGGGGGTCG GCTTAACAAC AGCAAACAGG GTTTTATCGG TGCTGGGCGG 
AGGGCCAAGG AGGACCAGGC GTTGCTATCT GTGCGGCCCC TGCAGGCTCA TGAGCTGAAC 
GACTTTCACG GTGTGGCCCC CCTCCACTTG CCGCATATCT GTAGCATCTG TGACAAGAAG 
GTGTTTGATT TGAAGGACTG GGAGCTGCAT GTGAAAGGGA AGCTGCACGC TCAGAAATGC 
CTGGTCTTCT CTGAAAATGC TGGCATCCGG TGTATACTTG GTTCGGCAGA GGGAACATTG 
TGTGCTTCTC CCAACAGCAC AGCTGTTTAT AACCCTGCTG GGAATGAAGA TTATGCCTCA 
AATCTTGGAA CATCATACGT GCCCATTCCA GCAAGGTCAT TCACTCAGTC AAGCCCCACA 
TTTCCTTTGG CTTCTGTGGG GACAACTTTT GCACAGCGGA AAGGGGCTGG CCGTGTGGTG 
CACATCTGCA ATCTCCCTGA AGGAAGCTGC ACTGAGAATG ACGTCATTAA CCTGGGGCTG 
CCCTTTGGAA AGGTCACTAA TTACATCCTC ATGAAGTCGA CTAATCAGGC CTTTTTAGAG 
ATGGCTTACA CAGAAGCTGC ACAGGCCATG GTCCAGTATT ATCAAGAAAA ATCTGCTGTG 
ATCAATGGTG AGAAGTTGCT CATTCGGATG TCCAAGAGAT ACAAGGAATT GCAGCTCAAG 
AAACCCGGGA AGGCCGTGGC TGCCATCATC CAGGACATCC ATTCCCAGAG GGAGAGGGAC 
ATGTTCCGGG AAGCAGACAG ATATGGCCCA GAAAGGCCGC GGTCTCGTAG TCCGGTGAGC 
CGGTCACTCT CCCCGAGGTC CCACACTCCC AGCTTCACCT CCTGCAGCTC TTCCCACAGC 
CCTCCGGGCC CCTCCCGGGC TGACTGGGGC AATGGCCGGG ACTCCTGGGA GCACTCTCCC 
TATGCCAGGA GGGAGGAAGA GCGAGACCCG GCTCCCTGGA GGGACAACGG AGATGACAAG 
AGGGACAGGA TGGACCCCTG GGCACATGAT CGCAAACACC ACCCCCGGCA ACTGGACAAG 
GCTGAGTTGG ACGAGCGACC AGAAGGAGGG AGGCCCCACC GGGAGAAGTA CCCGAGATCT 
GGGTCTCCCA ACCTGCCCCA CTCTGTGTCC AGCTACAAAA GCCGTGAAGA CGGCTACTAC 
CGGAAAGAGC CCAAAGCCAA GTCGGACAAG TATCTGAAGC AGCAGCAGGA TGCCCCCGGG 
AGGTCCAGGA GGAAAGACGA GGCCAGGCTG CGGGAAAGCA GACACCCCCA TCCGGATGAC 
TCAGGCAAGG AAGATGGGCT GGGGCCAAAG GTCACTAGGG CCCCTGAGGG CGCCAAGGCC 
AAGCAGAATG AGAAAAATAA AACCAAGAGA ACTGATAGAG ACCAAGAAGG AGCTGATGAT 
AGAAAAGAAA ACACAATGGC AGAGAATGAG GCTGGAAAAG AGGAACAGGA GGGCATGGAA 
GAAAGCCCTC AATCAGTGGG CAGACAGGAG AAAGAAGCAG AGTTCTCTGA TCCGGAAAAC 
ACAAGGACAA AGAAGGAACA AGATTGGGAG AGTGAAAGTG AGGCAGAGGG GGAGAGCTGG 
TATCCCACTA ACATGGAGGA GCTGGTGACA GTGGACGAGG TTGGGGAAGA AGAAGATTTT 
ATCGTGGAAC CAGACATCCC AGAGCTGGAA GAAATTGTGC CCATTGACCA GAAAGACAAA 
ATTTGCCCAG AAACATGTCT GTGTGTGACA ACCACCTTAG ACTTAGACCT GGCCCAGGAT 
TTCCCCAAGG AAGGAGTCAA GGCCGTAGGG AATGGGGCTG CAGAAATCAG CCTCAAGTCA 
CCCAGAGAAC TGCCCTCTGC TTCCACAAGC TGTCCCAGTG ACATGGACGT GGAAATGCCT 
GGCCTAAATC TGGATGCTGA GCGGAAGCCA GCTGAAAGTG AGACAGGCCT CTCCCTGGAG 
GATTCAGATT GCTACGAGAA GGAGGCAAAG GGAGTGGAGA GCTCAGATGT TCATCCAGCC 
CCTACAGTCC AGCAAATGTC TTCCCCTAAG CCAGCAGAGG AGAGGGCCCG GCAGCCAAGC 
CCATTTGTGG ATGATTGCAA GACCAGGGGG ACCCCCGAAG ATGGGGCTTG TGAAGGCAGC 
CCCCTGGAGG AGAAAGCCAG CCCCCCCATC GAAACTGACC TCCAAAACCA AGCTTGCCAA 
GAAGTGTTGA CCCCGGAAAA CTCCAGGTAC GTGGAAATGA AATCTCTGGA GGTGAGGTCA 
CCAGAGTACA CTGAAGTGGA ACTGAAACAG CCCCTTTCTT TGCCCTCTTG GGAACCAGAG 
GATGTGTTCA GTGAACTTAG CATTCCTCTA GGGGTGGAGT TCGTGGTTCC CAGGACTGGC 
TTTTATTGCA AGCTGTGTGG GCTGTTCTAC ACGAGCGAGG AGACAGCAAA GATGAGCCAC 
TGCCGCAGCG CTGTCCACTA CAGGAACTTA CAGAAATATT TGTCCCAGCT GGCCGAGGAG 
GGCCTCAAGG AGACCGAGGG GGCAGATAGC CCGAGGCCAG AGGACAGCGG AATCGTGCCA 
CGCTTCGAAA GGAAAAAGCT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78531
Clone ID Related Accession (Same CDS sequence) XM_017016104.1 , XM_011539697.2 , XM_017016104.2
Accession Version XM_017016104.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product RNA-binding protein 20 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCCCAGC CTCTCTTCAA TCAACTGAGG CATCCGTCTG TGATCACTGG CCCCCACGGC 
CATGCTGGGG TTCCCCAACA TGCTGCAGCC ATACCCAGTA CCCGGTTTCC CTCTAATGCA 
ATTGCCTTTT CACCCCCCAG CCAGACACGA GGCCCCGGAC CCTCCATGAA CCTTCCCAAC 
CAGCCACCCA GTGCCATGGT GATGCATCCT TTCACTGGGG TAATGCCTCA GACCCCTGGC 
CAGCCAGCAG TCATCTTGGG CATTGGCAAG ACTGGGCCTG CTCCAGCTAC AGCAGGATTC 
TATGAGTATG GCAAAGCCAG CTCTGGCCAG ACATATGGCC CTGAAACAGA TGGTCAGCCT 
GGCTTCCTGC CATCCTCGGC CTCAACCTCG GGCAGTGTGA CCTATGAAGG GCACTACAGC 
CACACAGGGC AGGATGGTCA AGCTGCCTTT TCCAAAGATT TTTACGGACC CAACTCCCAA 
GGTTCACATG TGGCCAGCGG ATTTCCAGCT GAGCAGGCTG GGGGCCTGAA AAGTGAGGTC 
GGGCCACTGC TGCAGGGCAC AAACAGCCAA TGGGAGAGCC CCCATGGATT CTCGGGCCAA 
AGCAAGCCTG ATCTCACAGC AGGTCCCATG TGGCCTCCAC CCCACAACCA GCCCTATGAG 
CTGTACGACC CCGAGGAACC AACCTCAGAC AGGACACCTC CTTCCTTCGG GGGTCGGCTT 
AACAACAGCA AACAGGGTTT TATCGGTGCT GGGCGGAGGG CCAAGGAGGA CCAGGCGTTG 
CTATCTGTGC GGCCCCTGCA GGCTCATGAG CTGAACGACT TTCACGGTGT GGCCCCCCTC 
CACTTGCCGC ATATCTGTAG CATCTGTGAC AAGAAGGTGT TTGATTTGAA GGACTGGGAG 
CTGCATGTGA AAGGGAAGCT GCACGCTCAG AAATGCCTGG TCTTCTCTGA AAATGCTGGC 
ATCCGGTGTA TACTTGGTTC GGCAGAGGGA ACATTGTGTG CTTCTCCCAA CAGCACAGCT 
GTTTATAACC CTGCTGGGAA TGAAGATTAT GCCTCAAATC TTGGAACATC ATACGTGCCC 
ATTCCAGCAA GGTCATTCAC TCAGTCAAGC CCCACATTTC CTTTGGCTTC TGTGGGGACA 
ACTTTTGCAC AGCGGAAAGG GGCTGGCCGT GTGGTGCACA TCTGCAATCT CCCTGAAGGA 
AGCTGCACTG AGAATGACGT CATTAACCTG GGGCTGCCCT TTGGAAAGGT CACTAATTAC 
ATCCTCATGA AGTCGACTAA TCAGGCCTTT TTAGAGATGG CTTACACAGA AGCTGCACAG 
GCCATGGTCC AGTATTATCA AGAAAAATCT GCTGTGATCA ATGGTGAGAA GTTGCTCATT 
CGGATGTCCA AGAGATACAA GGAATTGCAG CTCAAGAAAC CCGGGAAGGC CGTGGCTGCC 
ATCATCCAGG ACATCCATTC CCAGAGGGAG AGGGACATGT TCCGGGAAGC AGACAGATAT 
GGCCCAGAAA GGCCGCGGTC TCGTAGTCCG GTGAGCCGGT CACTCTCCCC GAGGTCCCAC 
ACTCCCAGCT TCACCTCCTG CAGCTCTTCC CACAGCCCTC CGGGCCCCTC CCGGGCTGAC 
TGGGGCAATG GCCGGGACTC CTGGGAGCAC TCTCCCTATG CCAGGAGGGA GGAAGAGCGA 
GACCCGGCTC CCTGGAGGGA CAACGGAGAT GACAAGAGGG ACAGGATGGA CCCCTGGGCA 
CATGATCGCA AACACCACCC CCGGCAACTG GACAAGGCTG AGTTGGACGA GCGACCAGAA 
GGAGGGAGGC CCCACCGGGA GAAGTACCCG AGATCTGGGT CTCCCAACCT GCCCCACTCT 
GTGTCCAGCT ACAAAAGCCG TGAAGACGGC TACTACCGGA AAGAGCCCAA AGCCAAGTCG 
GACAAGTATC TGAAGCAGCA GCAGGATGCC CCCGGGAGGT CCAGGAGGAA AGACGAGGCC 
AGGCTGCGGG AAAGCAGACA CCCCCATCCG GATGACTCAG GCAAGGAAGA TGGGCTGGGG 
CCAAAGGTCA CTAGGGCCCC TGAGGGCGCC AAGGCCAAGC AGAATGAGAA AAATAAAACC 
AAGAGAACTG ATAGAGACCA AGAAGGAGCT GATGATAGAA AAGAAAACAC AATGGCAGAG 
AATGAGGCTG GAAAAGAGGA ACAGGAGGGC ATGGAAGAAA GCCCTCAATC AGTGGGCAGA 
CAGGAGAAAG AAGCAGAGTT CTCTGATCCG GAAAACACAA GGACAAAGAA GGAACAAGAT 
TGGGAGAGTG AAAGTGAGGC AGAGGGGGAG AGCTGGTATC CCACTAACAT GGAGGAGCTG 
GTGACAGTGG ACGAGGTTGG GGAAGAAGAA GATTTTATCG TGGAACCAGA CATCCCAGAG 
CTGGAAGAAA TTGTGCCCAT TGACCAGAAA GACAAAATTT GCCCAGAAAC ATGTCTGTGT 
GTGACAACCA CCTTAGACTT AGACCTGGCC CAGGATTTCC CCAAGGAAGG AGTCAAGGCC 
GTAGGGAATG GGGCTGCAGA AATCAGCCTC AAGTCACCCA GAGAACTGCC CTCTGCTTCC 
ACAAGCTGTC CCAGTGACAT GGACGTGGAA ATGCCTGGCC TAAATCTGGA TGCTGAGCGG 
AAGCCAGCTG AAAGTGAGAC AGGCCTCTCC CTGGAGGATT CAGATTGCTA CGAGAAGGAG 
GCAAAGGGAG TGGAGAGCTC AGATGTTCAT CCAGCCCCTA CAGTCCAGCA AATGTCTTCC 
CCTAAGCCAG CAGAGGAGAG GGCCCGGCAG CCAAGCCCAT TTGTGGATGA TTGCAAGACC 
AGGGGGACCC CCGAAGATGG GGCTTGTGAA GGCAGCCCCC TGGAGGAGAA AGCCAGCCCC 
CCCATCGAAA CTGACCTCCA AAACCAAGCT TGCCAAGAAG TGTTGACCCC GGAAAACTCC 
AGGTACGTGG AAATGAAATC TCTGGAGGTG AGGTCACCAG AGTACACTGA AGTGGAACTG 
AAACAGCCCC TTTCTTTGCC CTCTTGGGAA CCAGAGGATG TGTTCAGTGA ACTTAGCATT 
CCTCTAGGGG TGGAGTTCGT GGTTCCCAGG ACTGGCTTTT ATTGCAAGCT GTGTGGGCTG 
TTCTACACGA GCGAGGAGAC AGCAAAGATG AGCCACTGCC GCAGCGCTGT CCACTACAGG 
AACTTACAGA AATATTTGTC CCAGCTGGCC GAGGAGGGCC TCAAGGAGAC CGAGGGGGCA 
GATAGCCCGA GGCCAGAGGA CAGCGGAATC GTGCCACGCT TCGAAAGGAA AAAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016871593.1
CDS3917..7216
Translation

Target ORF information:

RefSeq Version XM_017016104.1
Organism Homo sapiens(human)
Definition Homo sapiens RNA binding motif protein 20 (RBM20), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017016104.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCCCAGC CTCTCTTCAA TCAACTGAGG CATCCGTCTG TGATCACTGG CCCCCACGGC 
CATGCTGGGG TTCCCCAACA TGCTGCAGCC ATACCCAGTA CCCGGTTTCC CTCTAATGCA 
ATTGCCTTTT CACCCCCCAG CCAGACACGA GGCCCCGGAC CCTCCATGAA CCTTCCCAAC 
CAGCCACCCA GTGCCATGGT GATGCATCCT TTCACTGGGG TAATGCCTCA GACCCCTGGC 
CAGCCAGCAG TCATCTTGGG CATTGGCAAG ACTGGGCCTG CTCCAGCTAC AGCAGGATTC 
TATGAGTATG GCAAAGCCAG CTCTGGCCAG ACATATGGCC CTGAAACAGA TGGTCAGCCT 
GGCTTCCTGC CATCCTCGGC CTCAACCTCG GGCAGTGTGA CCTATGAAGG GCACTACAGC 
CACACAGGGC AGGATGGTCA AGCTGCCTTT TCCAAAGATT TTTACGGACC CAACTCCCAA 
GGTTCACATG TGGCCAGCGG ATTTCCAGCT GAGCAGGCTG GGGGCCTGAA AAGTGAGGTC 
GGGCCACTGC TGCAGGGCAC AAACAGCCAA TGGGAGAGCC CCCATGGATT CTCGGGCCAA 
AGCAAGCCTG ATCTCACAGC AGGTCCCATG TGGCCTCCAC CCCACAACCA GCCCTATGAG 
CTGTACGACC CCGAGGAACC AACCTCAGAC AGGACACCTC CTTCCTTCGG GGGTCGGCTT 
AACAACAGCA AACAGGGTTT TATCGGTGCT GGGCGGAGGG CCAAGGAGGA CCAGGCGTTG 
CTATCTGTGC GGCCCCTGCA GGCTCATGAG CTGAACGACT TTCACGGTGT GGCCCCCCTC 
CACTTGCCGC ATATCTGTAG CATCTGTGAC AAGAAGGTGT TTGATTTGAA GGACTGGGAG 
CTGCATGTGA AAGGGAAGCT GCACGCTCAG AAATGCCTGG TCTTCTCTGA AAATGCTGGC 
ATCCGGTGTA TACTTGGTTC GGCAGAGGGA ACATTGTGTG CTTCTCCCAA CAGCACAGCT 
GTTTATAACC CTGCTGGGAA TGAAGATTAT GCCTCAAATC TTGGAACATC ATACGTGCCC 
ATTCCAGCAA GGTCATTCAC TCAGTCAAGC CCCACATTTC CTTTGGCTTC TGTGGGGACA 
ACTTTTGCAC AGCGGAAAGG GGCTGGCCGT GTGGTGCACA TCTGCAATCT CCCTGAAGGA 
AGCTGCACTG AGAATGACGT CATTAACCTG GGGCTGCCCT TTGGAAAGGT CACTAATTAC 
ATCCTCATGA AGTCGACTAA TCAGGCCTTT TTAGAGATGG CTTACACAGA AGCTGCACAG 
GCCATGGTCC AGTATTATCA AGAAAAATCT GCTGTGATCA ATGGTGAGAA GTTGCTCATT 
CGGATGTCCA AGAGATACAA GGAATTGCAG CTCAAGAAAC CCGGGAAGGC CGTGGCTGCC 
ATCATCCAGG ACATCCATTC CCAGAGGGAG AGGGACATGT TCCGGGAAGC AGACAGATAT 
GGCCCAGAAA GGCCGCGGTC TCGTAGTCCG GTGAGCCGGT CACTCTCCCC GAGGTCCCAC 
ACTCCCAGCT TCACCTCCTG CAGCTCTTCC CACAGCCCTC CGGGCCCCTC CCGGGCTGAC 
TGGGGCAATG GCCGGGACTC CTGGGAGCAC TCTCCCTATG CCAGGAGGGA GGAAGAGCGA 
GACCCGGCTC CCTGGAGGGA CAACGGAGAT GACAAGAGGG ACAGGATGGA CCCCTGGGCA 
CATGATCGCA AACACCACCC CCGGCAACTG GACAAGGCTG AGTTGGACGA GCGACCAGAA 
GGAGGGAGGC CCCACCGGGA GAAGTACCCG AGATCTGGGT CTCCCAACCT GCCCCACTCT 
GTGTCCAGCT ACAAAAGCCG TGAAGACGGC TACTACCGGA AAGAGCCCAA AGCCAAGTCG 
GACAAGTATC TGAAGCAGCA GCAGGATGCC CCCGGGAGGT CCAGGAGGAA AGACGAGGCC 
AGGCTGCGGG AAAGCAGACA CCCCCATCCG GATGACTCAG GCAAGGAAGA TGGGCTGGGG 
CCAAAGGTCA CTAGGGCCCC TGAGGGCGCC AAGGCCAAGC AGAATGAGAA AAATAAAACC 
AAGAGAACTG ATAGAGACCA AGAAGGAGCT GATGATAGAA AAGAAAACAC AATGGCAGAG 
AATGAGGCTG GAAAAGAGGA ACAGGAGGGC ATGGAAGAAA GCCCTCAATC AGTGGGCAGA 
CAGGAGAAAG AAGCAGAGTT CTCTGATCCG GAAAACACAA GGACAAAGAA GGAACAAGAT 
TGGGAGAGTG AAAGTGAGGC AGAGGGGGAG AGCTGGTATC CCACTAACAT GGAGGAGCTG 
GTGACAGTGG ACGAGGTTGG GGAAGAAGAA GATTTTATCG TGGAACCAGA CATCCCAGAG 
CTGGAAGAAA TTGTGCCCAT TGACCAGAAA GACAAAATTT GCCCAGAAAC ATGTCTGTGT 
GTGACAACCA CCTTAGACTT AGACCTGGCC CAGGATTTCC CCAAGGAAGG AGTCAAGGCC 
GTAGGGAATG GGGCTGCAGA AATCAGCCTC AAGTCACCCA GAGAACTGCC CTCTGCTTCC 
ACAAGCTGTC CCAGTGACAT GGACGTGGAA ATGCCTGGCC TAAATCTGGA TGCTGAGCGG 
AAGCCAGCTG AAAGTGAGAC AGGCCTCTCC CTGGAGGATT CAGATTGCTA CGAGAAGGAG 
GCAAAGGGAG TGGAGAGCTC AGATGTTCAT CCAGCCCCTA CAGTCCAGCA AATGTCTTCC 
CCTAAGCCAG CAGAGGAGAG GGCCCGGCAG CCAAGCCCAT TTGTGGATGA TTGCAAGACC 
AGGGGGACCC CCGAAGATGG GGCTTGTGAA GGCAGCCCCC TGGAGGAGAA AGCCAGCCCC 
CCCATCGAAA CTGACCTCCA AAACCAAGCT TGCCAAGAAG TGTTGACCCC GGAAAACTCC 
AGGTACGTGG AAATGAAATC TCTGGAGGTG AGGTCACCAG AGTACACTGA AGTGGAACTG 
AAACAGCCCC TTTCTTTGCC CTCTTGGGAA CCAGAGGATG TGTTCAGTGA ACTTAGCATT 
CCTCTAGGGG TGGAGTTCGT GGTTCCCAGG ACTGGCTTTT ATTGCAAGCT GTGTGGGCTG 
TTCTACACGA GCGAGGAGAC AGCAAAGATG AGCCACTGCC GCAGCGCTGT CCACTACAGG 
AACTTACAGA AATATTTGTC CCAGCTGGCC GAGGAGGGCC TCAAGGAGAC CGAGGGGGCA 
GATAGCCCGA GGCCAGAGGA CAGCGGAATC GTGCCACGCT TCGAAAGGAA AAAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78531
Clone ID Related Accession (Same CDS sequence) XM_017016104.1 , XM_011539697.2 , XM_017016104.2
Accession Version XM_011539697.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product RNA-binding protein 20 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539697.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCCCAGC CTCTCTTCAA TCAACTGAGG CATCCGTCTG TGATCACTGG CCCCCACGGC 
CATGCTGGGG TTCCCCAACA TGCTGCAGCC ATACCCAGTA CCCGGTTTCC CTCTAATGCA 
ATTGCCTTTT CACCCCCCAG CCAGACACGA GGCCCCGGAC CCTCCATGAA CCTTCCCAAC 
CAGCCACCCA GTGCCATGGT GATGCATCCT TTCACTGGGG TAATGCCTCA GACCCCTGGC 
CAGCCAGCAG TCATCTTGGG CATTGGCAAG ACTGGGCCTG CTCCAGCTAC AGCAGGATTC 
TATGAGTATG GCAAAGCCAG CTCTGGCCAG ACATATGGCC CTGAAACAGA TGGTCAGCCT 
GGCTTCCTGC CATCCTCGGC CTCAACCTCG GGCAGTGTGA CCTATGAAGG GCACTACAGC 
CACACAGGGC AGGATGGTCA AGCTGCCTTT TCCAAAGATT TTTACGGACC CAACTCCCAA 
GGTTCACATG TGGCCAGCGG ATTTCCAGCT GAGCAGGCTG GGGGCCTGAA AAGTGAGGTC 
GGGCCACTGC TGCAGGGCAC AAACAGCCAA TGGGAGAGCC CCCATGGATT CTCGGGCCAA 
AGCAAGCCTG ATCTCACAGC AGGTCCCATG TGGCCTCCAC CCCACAACCA GCCCTATGAG 
CTGTACGACC CCGAGGAACC AACCTCAGAC AGGACACCTC CTTCCTTCGG GGGTCGGCTT 
AACAACAGCA AACAGGGTTT TATCGGTGCT GGGCGGAGGG CCAAGGAGGA CCAGGCGTTG 
CTATCTGTGC GGCCCCTGCA GGCTCATGAG CTGAACGACT TTCACGGTGT GGCCCCCCTC 
CACTTGCCGC ATATCTGTAG CATCTGTGAC AAGAAGGTGT TTGATTTGAA GGACTGGGAG 
CTGCATGTGA AAGGGAAGCT GCACGCTCAG AAATGCCTGG TCTTCTCTGA AAATGCTGGC 
ATCCGGTGTA TACTTGGTTC GGCAGAGGGA ACATTGTGTG CTTCTCCCAA CAGCACAGCT 
GTTTATAACC CTGCTGGGAA TGAAGATTAT GCCTCAAATC TTGGAACATC ATACGTGCCC 
ATTCCAGCAA GGTCATTCAC TCAGTCAAGC CCCACATTTC CTTTGGCTTC TGTGGGGACA 
ACTTTTGCAC AGCGGAAAGG GGCTGGCCGT GTGGTGCACA TCTGCAATCT CCCTGAAGGA 
AGCTGCACTG AGAATGACGT CATTAACCTG GGGCTGCCCT TTGGAAAGGT CACTAATTAC 
ATCCTCATGA AGTCGACTAA TCAGGCCTTT TTAGAGATGG CTTACACAGA AGCTGCACAG 
GCCATGGTCC AGTATTATCA AGAAAAATCT GCTGTGATCA ATGGTGAGAA GTTGCTCATT 
CGGATGTCCA AGAGATACAA GGAATTGCAG CTCAAGAAAC CCGGGAAGGC CGTGGCTGCC 
ATCATCCAGG ACATCCATTC CCAGAGGGAG AGGGACATGT TCCGGGAAGC AGACAGATAT 
GGCCCAGAAA GGCCGCGGTC TCGTAGTCCG GTGAGCCGGT CACTCTCCCC GAGGTCCCAC 
ACTCCCAGCT TCACCTCCTG CAGCTCTTCC CACAGCCCTC CGGGCCCCTC CCGGGCTGAC 
TGGGGCAATG GCCGGGACTC CTGGGAGCAC TCTCCCTATG CCAGGAGGGA GGAAGAGCGA 
GACCCGGCTC CCTGGAGGGA CAACGGAGAT GACAAGAGGG ACAGGATGGA CCCCTGGGCA 
CATGATCGCA AACACCACCC CCGGCAACTG GACAAGGCTG AGTTGGACGA GCGACCAGAA 
GGAGGGAGGC CCCACCGGGA GAAGTACCCG AGATCTGGGT CTCCCAACCT GCCCCACTCT 
GTGTCCAGCT ACAAAAGCCG TGAAGACGGC TACTACCGGA AAGAGCCCAA AGCCAAGTCG 
GACAAGTATC TGAAGCAGCA GCAGGATGCC CCCGGGAGGT CCAGGAGGAA AGACGAGGCC 
AGGCTGCGGG AAAGCAGACA CCCCCATCCG GATGACTCAG GCAAGGAAGA TGGGCTGGGG 
CCAAAGGTCA CTAGGGCCCC TGAGGGCGCC AAGGCCAAGC AGAATGAGAA AAATAAAACC 
AAGAGAACTG ATAGAGACCA AGAAGGAGCT GATGATAGAA AAGAAAACAC AATGGCAGAG 
AATGAGGCTG GAAAAGAGGA ACAGGAGGGC ATGGAAGAAA GCCCTCAATC AGTGGGCAGA 
CAGGAGAAAG AAGCAGAGTT CTCTGATCCG GAAAACACAA GGACAAAGAA GGAACAAGAT 
TGGGAGAGTG AAAGTGAGGC AGAGGGGGAG AGCTGGTATC CCACTAACAT GGAGGAGCTG 
GTGACAGTGG ACGAGGTTGG GGAAGAAGAA GATTTTATCG TGGAACCAGA CATCCCAGAG 
CTGGAAGAAA TTGTGCCCAT TGACCAGAAA GACAAAATTT GCCCAGAAAC ATGTCTGTGT 
GTGACAACCA CCTTAGACTT AGACCTGGCC CAGGATTTCC CCAAGGAAGG AGTCAAGGCC 
GTAGGGAATG GGGCTGCAGA AATCAGCCTC AAGTCACCCA GAGAACTGCC CTCTGCTTCC 
ACAAGCTGTC CCAGTGACAT GGACGTGGAA ATGCCTGGCC TAAATCTGGA TGCTGAGCGG 
AAGCCAGCTG AAAGTGAGAC AGGCCTCTCC CTGGAGGATT CAGATTGCTA CGAGAAGGAG 
GCAAAGGGAG TGGAGAGCTC AGATGTTCAT CCAGCCCCTA CAGTCCAGCA AATGTCTTCC 
CCTAAGCCAG CAGAGGAGAG GGCCCGGCAG CCAAGCCCAT TTGTGGATGA TTGCAAGACC 
AGGGGGACCC CCGAAGATGG GGCTTGTGAA GGCAGCCCCC TGGAGGAGAA AGCCAGCCCC 
CCCATCGAAA CTGACCTCCA AAACCAAGCT TGCCAAGAAG TGTTGACCCC GGAAAACTCC 
AGGTACGTGG AAATGAAATC TCTGGAGGTG AGGTCACCAG AGTACACTGA AGTGGAACTG 
AAACAGCCCC TTTCTTTGCC CTCTTGGGAA CCAGAGGATG TGTTCAGTGA ACTTAGCATT 
CCTCTAGGGG TGGAGTTCGT GGTTCCCAGG ACTGGCTTTT ATTGCAAGCT GTGTGGGCTG 
TTCTACACGA GCGAGGAGAC AGCAAAGATG AGCCACTGCC GCAGCGCTGT CCACTACAGG 
AACTTACAGA AATATTTGTC CCAGCTGGCC GAGGAGGGCC TCAAGGAGAC CGAGGGGGCA 
GATAGCCCGA GGCCAGAGGA CAGCGGAATC GTGCCACGCT TCGAAAGGAA AAAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011537999.1
CDS281..3580
Translation

Target ORF information:

RefSeq Version XM_011539697.2
Organism Homo sapiens(human)
Definition Homo sapiens RNA binding motif protein 20 (RBM20), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011539697.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCCCAGC CTCTCTTCAA TCAACTGAGG CATCCGTCTG TGATCACTGG CCCCCACGGC 
CATGCTGGGG TTCCCCAACA TGCTGCAGCC ATACCCAGTA CCCGGTTTCC CTCTAATGCA 
ATTGCCTTTT CACCCCCCAG CCAGACACGA GGCCCCGGAC CCTCCATGAA CCTTCCCAAC 
CAGCCACCCA GTGCCATGGT GATGCATCCT TTCACTGGGG TAATGCCTCA GACCCCTGGC 
CAGCCAGCAG TCATCTTGGG CATTGGCAAG ACTGGGCCTG CTCCAGCTAC AGCAGGATTC 
TATGAGTATG GCAAAGCCAG CTCTGGCCAG ACATATGGCC CTGAAACAGA TGGTCAGCCT 
GGCTTCCTGC CATCCTCGGC CTCAACCTCG GGCAGTGTGA CCTATGAAGG GCACTACAGC 
CACACAGGGC AGGATGGTCA AGCTGCCTTT TCCAAAGATT TTTACGGACC CAACTCCCAA 
GGTTCACATG TGGCCAGCGG ATTTCCAGCT GAGCAGGCTG GGGGCCTGAA AAGTGAGGTC 
GGGCCACTGC TGCAGGGCAC AAACAGCCAA TGGGAGAGCC CCCATGGATT CTCGGGCCAA 
AGCAAGCCTG ATCTCACAGC AGGTCCCATG TGGCCTCCAC CCCACAACCA GCCCTATGAG 
CTGTACGACC CCGAGGAACC AACCTCAGAC AGGACACCTC CTTCCTTCGG GGGTCGGCTT 
AACAACAGCA AACAGGGTTT TATCGGTGCT GGGCGGAGGG CCAAGGAGGA CCAGGCGTTG 
CTATCTGTGC GGCCCCTGCA GGCTCATGAG CTGAACGACT TTCACGGTGT GGCCCCCCTC 
CACTTGCCGC ATATCTGTAG CATCTGTGAC AAGAAGGTGT TTGATTTGAA GGACTGGGAG 
CTGCATGTGA AAGGGAAGCT GCACGCTCAG AAATGCCTGG TCTTCTCTGA AAATGCTGGC 
ATCCGGTGTA TACTTGGTTC GGCAGAGGGA ACATTGTGTG CTTCTCCCAA CAGCACAGCT 
GTTTATAACC CTGCTGGGAA TGAAGATTAT GCCTCAAATC TTGGAACATC ATACGTGCCC 
ATTCCAGCAA GGTCATTCAC TCAGTCAAGC CCCACATTTC CTTTGGCTTC TGTGGGGACA 
ACTTTTGCAC AGCGGAAAGG GGCTGGCCGT GTGGTGCACA TCTGCAATCT CCCTGAAGGA 
AGCTGCACTG AGAATGACGT CATTAACCTG GGGCTGCCCT TTGGAAAGGT CACTAATTAC 
ATCCTCATGA AGTCGACTAA TCAGGCCTTT TTAGAGATGG CTTACACAGA AGCTGCACAG 
GCCATGGTCC AGTATTATCA AGAAAAATCT GCTGTGATCA ATGGTGAGAA GTTGCTCATT 
CGGATGTCCA AGAGATACAA GGAATTGCAG CTCAAGAAAC CCGGGAAGGC CGTGGCTGCC 
ATCATCCAGG ACATCCATTC CCAGAGGGAG AGGGACATGT TCCGGGAAGC AGACAGATAT 
GGCCCAGAAA GGCCGCGGTC TCGTAGTCCG GTGAGCCGGT CACTCTCCCC GAGGTCCCAC 
ACTCCCAGCT TCACCTCCTG CAGCTCTTCC CACAGCCCTC CGGGCCCCTC CCGGGCTGAC 
TGGGGCAATG GCCGGGACTC CTGGGAGCAC TCTCCCTATG CCAGGAGGGA GGAAGAGCGA 
GACCCGGCTC CCTGGAGGGA CAACGGAGAT GACAAGAGGG ACAGGATGGA CCCCTGGGCA 
CATGATCGCA AACACCACCC CCGGCAACTG GACAAGGCTG AGTTGGACGA GCGACCAGAA 
GGAGGGAGGC CCCACCGGGA GAAGTACCCG AGATCTGGGT CTCCCAACCT GCCCCACTCT 
GTGTCCAGCT ACAAAAGCCG TGAAGACGGC TACTACCGGA AAGAGCCCAA AGCCAAGTCG 
GACAAGTATC TGAAGCAGCA GCAGGATGCC CCCGGGAGGT CCAGGAGGAA AGACGAGGCC 
AGGCTGCGGG AAAGCAGACA CCCCCATCCG GATGACTCAG GCAAGGAAGA TGGGCTGGGG 
CCAAAGGTCA CTAGGGCCCC TGAGGGCGCC AAGGCCAAGC AGAATGAGAA AAATAAAACC 
AAGAGAACTG ATAGAGACCA AGAAGGAGCT GATGATAGAA AAGAAAACAC AATGGCAGAG 
AATGAGGCTG GAAAAGAGGA ACAGGAGGGC ATGGAAGAAA GCCCTCAATC AGTGGGCAGA 
CAGGAGAAAG AAGCAGAGTT CTCTGATCCG GAAAACACAA GGACAAAGAA GGAACAAGAT 
TGGGAGAGTG AAAGTGAGGC AGAGGGGGAG AGCTGGTATC CCACTAACAT GGAGGAGCTG 
GTGACAGTGG ACGAGGTTGG GGAAGAAGAA GATTTTATCG TGGAACCAGA CATCCCAGAG 
CTGGAAGAAA TTGTGCCCAT TGACCAGAAA GACAAAATTT GCCCAGAAAC ATGTCTGTGT 
GTGACAACCA CCTTAGACTT AGACCTGGCC CAGGATTTCC CCAAGGAAGG AGTCAAGGCC 
GTAGGGAATG GGGCTGCAGA AATCAGCCTC AAGTCACCCA GAGAACTGCC CTCTGCTTCC 
ACAAGCTGTC CCAGTGACAT GGACGTGGAA ATGCCTGGCC TAAATCTGGA TGCTGAGCGG 
AAGCCAGCTG AAAGTGAGAC AGGCCTCTCC CTGGAGGATT CAGATTGCTA CGAGAAGGAG 
GCAAAGGGAG TGGAGAGCTC AGATGTTCAT CCAGCCCCTA CAGTCCAGCA AATGTCTTCC 
CCTAAGCCAG CAGAGGAGAG GGCCCGGCAG CCAAGCCCAT TTGTGGATGA TTGCAAGACC 
AGGGGGACCC CCGAAGATGG GGCTTGTGAA GGCAGCCCCC TGGAGGAGAA AGCCAGCCCC 
CCCATCGAAA CTGACCTCCA AAACCAAGCT TGCCAAGAAG TGTTGACCCC GGAAAACTCC 
AGGTACGTGG AAATGAAATC TCTGGAGGTG AGGTCACCAG AGTACACTGA AGTGGAACTG 
AAACAGCCCC TTTCTTTGCC CTCTTGGGAA CCAGAGGATG TGTTCAGTGA ACTTAGCATT 
CCTCTAGGGG TGGAGTTCGT GGTTCCCAGG ACTGGCTTTT ATTGCAAGCT GTGTGGGCTG 
TTCTACACGA GCGAGGAGAC AGCAAAGATG AGCCACTGCC GCAGCGCTGT CCACTACAGG 
AACTTACAGA AATATTTGTC CCAGCTGGCC GAGGAGGGCC TCAAGGAGAC CGAGGGGGCA 
GATAGCCCGA GGCCAGAGGA CAGCGGAATC GTGCCACGCT TCGAAAGGAA AAAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78531
Clone ID Related Accession (Same CDS sequence) XM_017016104.1 , XM_011539697.2 , XM_017016104.2
Accession Version XM_017016104.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3300bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product RNA-binding protein 20 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016104.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCCCAGC CTCTCTTCAA TCAACTGAGG CATCCGTCTG TGATCACTGG CCCCCACGGC 
CATGCTGGGG TTCCCCAACA TGCTGCAGCC ATACCCAGTA CCCGGTTTCC CTCTAATGCA 
ATTGCCTTTT CACCCCCCAG CCAGACACGA GGCCCCGGAC CCTCCATGAA CCTTCCCAAC 
CAGCCACCCA GTGCCATGGT GATGCATCCT TTCACTGGGG TAATGCCTCA GACCCCTGGC 
CAGCCAGCAG TCATCTTGGG CATTGGCAAG ACTGGGCCTG CTCCAGCTAC AGCAGGATTC 
TATGAGTATG GCAAAGCCAG CTCTGGCCAG ACATATGGCC CTGAAACAGA TGGTCAGCCT 
GGCTTCCTGC CATCCTCGGC CTCAACCTCG GGCAGTGTGA CCTATGAAGG GCACTACAGC 
CACACAGGGC AGGATGGTCA AGCTGCCTTT TCCAAAGATT TTTACGGACC CAACTCCCAA 
GGTTCACATG TGGCCAGCGG ATTTCCAGCT GAGCAGGCTG GGGGCCTGAA AAGTGAGGTC 
GGGCCACTGC TGCAGGGCAC AAACAGCCAA TGGGAGAGCC CCCATGGATT CTCGGGCCAA 
AGCAAGCCTG ATCTCACAGC AGGTCCCATG TGGCCTCCAC CCCACAACCA GCCCTATGAG 
CTGTACGACC CCGAGGAACC AACCTCAGAC AGGACACCTC CTTCCTTCGG GGGTCGGCTT 
AACAACAGCA AACAGGGTTT TATCGGTGCT GGGCGGAGGG CCAAGGAGGA CCAGGCGTTG 
CTATCTGTGC GGCCCCTGCA GGCTCATGAG CTGAACGACT TTCACGGTGT GGCCCCCCTC 
CACTTGCCGC ATATCTGTAG CATCTGTGAC AAGAAGGTGT TTGATTTGAA GGACTGGGAG 
CTGCATGTGA AAGGGAAGCT GCACGCTCAG AAATGCCTGG TCTTCTCTGA AAATGCTGGC 
ATCCGGTGTA TACTTGGTTC GGCAGAGGGA ACATTGTGTG CTTCTCCCAA CAGCACAGCT 
GTTTATAACC CTGCTGGGAA TGAAGATTAT GCCTCAAATC TTGGAACATC ATACGTGCCC 
ATTCCAGCAA GGTCATTCAC TCAGTCAAGC CCCACATTTC CTTTGGCTTC TGTGGGGACA 
ACTTTTGCAC AGCGGAAAGG GGCTGGCCGT GTGGTGCACA TCTGCAATCT CCCTGAAGGA 
AGCTGCACTG AGAATGACGT CATTAACCTG GGGCTGCCCT TTGGAAAGGT CACTAATTAC 
ATCCTCATGA AGTCGACTAA TCAGGCCTTT TTAGAGATGG CTTACACAGA AGCTGCACAG 
GCCATGGTCC AGTATTATCA AGAAAAATCT GCTGTGATCA ATGGTGAGAA GTTGCTCATT 
CGGATGTCCA AGAGATACAA GGAATTGCAG CTCAAGAAAC CCGGGAAGGC CGTGGCTGCC 
ATCATCCAGG ACATCCATTC CCAGAGGGAG AGGGACATGT TCCGGGAAGC AGACAGATAT 
GGCCCAGAAA GGCCGCGGTC TCGTAGTCCG GTGAGCCGGT CACTCTCCCC GAGGTCCCAC 
ACTCCCAGCT TCACCTCCTG CAGCTCTTCC CACAGCCCTC CGGGCCCCTC CCGGGCTGAC 
TGGGGCAATG GCCGGGACTC CTGGGAGCAC TCTCCCTATG CCAGGAGGGA GGAAGAGCGA 
GACCCGGCTC CCTGGAGGGA CAACGGAGAT GACAAGAGGG ACAGGATGGA CCCCTGGGCA 
CATGATCGCA AACACCACCC CCGGCAACTG GACAAGGCTG AGTTGGACGA GCGACCAGAA 
GGAGGGAGGC CCCACCGGGA GAAGTACCCG AGATCTGGGT CTCCCAACCT GCCCCACTCT 
GTGTCCAGCT ACAAAAGCCG TGAAGACGGC TACTACCGGA AAGAGCCCAA AGCCAAGTCG 
GACAAGTATC TGAAGCAGCA GCAGGATGCC CCCGGGAGGT CCAGGAGGAA AGACGAGGCC 
AGGCTGCGGG AAAGCAGACA CCCCCATCCG GATGACTCAG GCAAGGAAGA TGGGCTGGGG 
CCAAAGGTCA CTAGGGCCCC TGAGGGCGCC AAGGCCAAGC AGAATGAGAA AAATAAAACC 
AAGAGAACTG ATAGAGACCA AGAAGGAGCT GATGATAGAA AAGAAAACAC AATGGCAGAG 
AATGAGGCTG GAAAAGAGGA ACAGGAGGGC ATGGAAGAAA GCCCTCAATC AGTGGGCAGA 
CAGGAGAAAG AAGCAGAGTT CTCTGATCCG GAAAACACAA GGACAAAGAA GGAACAAGAT 
TGGGAGAGTG AAAGTGAGGC AGAGGGGGAG AGCTGGTATC CCACTAACAT GGAGGAGCTG 
GTGACAGTGG ACGAGGTTGG GGAAGAAGAA GATTTTATCG TGGAACCAGA CATCCCAGAG 
CTGGAAGAAA TTGTGCCCAT TGACCAGAAA GACAAAATTT GCCCAGAAAC ATGTCTGTGT 
GTGACAACCA CCTTAGACTT AGACCTGGCC CAGGATTTCC CCAAGGAAGG AGTCAAGGCC 
GTAGGGAATG GGGCTGCAGA AATCAGCCTC AAGTCACCCA GAGAACTGCC CTCTGCTTCC 
ACAAGCTGTC CCAGTGACAT GGACGTGGAA ATGCCTGGCC TAAATCTGGA TGCTGAGCGG 
AAGCCAGCTG AAAGTGAGAC AGGCCTCTCC CTGGAGGATT CAGATTGCTA CGAGAAGGAG 
GCAAAGGGAG TGGAGAGCTC AGATGTTCAT CCAGCCCCTA CAGTCCAGCA AATGTCTTCC 
CCTAAGCCAG CAGAGGAGAG GGCCCGGCAG CCAAGCCCAT TTGTGGATGA TTGCAAGACC 
AGGGGGACCC CCGAAGATGG GGCTTGTGAA GGCAGCCCCC TGGAGGAGAA AGCCAGCCCC 
CCCATCGAAA CTGACCTCCA AAACCAAGCT TGCCAAGAAG TGTTGACCCC GGAAAACTCC 
AGGTACGTGG AAATGAAATC TCTGGAGGTG AGGTCACCAG AGTACACTGA AGTGGAACTG 
AAACAGCCCC TTTCTTTGCC CTCTTGGGAA CCAGAGGATG TGTTCAGTGA ACTTAGCATT 
CCTCTAGGGG TGGAGTTCGT GGTTCCCAGG ACTGGCTTTT ATTGCAAGCT GTGTGGGCTG 
TTCTACACGA GCGAGGAGAC AGCAAAGATG AGCCACTGCC GCAGCGCTGT CCACTACAGG 
AACTTACAGA AATATTTGTC CCAGCTGGCC GAGGAGGGCC TCAAGGAGAC CGAGGGGGCA 
GATAGCCCGA GGCCAGAGGA CAGCGGAATC GTGCCACGCT TCGAAAGGAA AAAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016871593.1
CDS3919..7218
Translation

Target ORF information:

RefSeq Version XM_017016104.2
Organism Homo sapiens(human)
Definition Homo sapiens RNA binding motif protein 20 (RBM20), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017016104.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGTCCCAGC CTCTCTTCAA TCAACTGAGG CATCCGTCTG TGATCACTGG CCCCCACGGC 
CATGCTGGGG TTCCCCAACA TGCTGCAGCC ATACCCAGTA CCCGGTTTCC CTCTAATGCA 
ATTGCCTTTT CACCCCCCAG CCAGACACGA GGCCCCGGAC CCTCCATGAA CCTTCCCAAC 
CAGCCACCCA GTGCCATGGT GATGCATCCT TTCACTGGGG TAATGCCTCA GACCCCTGGC 
CAGCCAGCAG TCATCTTGGG CATTGGCAAG ACTGGGCCTG CTCCAGCTAC AGCAGGATTC 
TATGAGTATG GCAAAGCCAG CTCTGGCCAG ACATATGGCC CTGAAACAGA TGGTCAGCCT 
GGCTTCCTGC CATCCTCGGC CTCAACCTCG GGCAGTGTGA CCTATGAAGG GCACTACAGC 
CACACAGGGC AGGATGGTCA AGCTGCCTTT TCCAAAGATT TTTACGGACC CAACTCCCAA 
GGTTCACATG TGGCCAGCGG ATTTCCAGCT GAGCAGGCTG GGGGCCTGAA AAGTGAGGTC 
GGGCCACTGC TGCAGGGCAC AAACAGCCAA TGGGAGAGCC CCCATGGATT CTCGGGCCAA 
AGCAAGCCTG ATCTCACAGC AGGTCCCATG TGGCCTCCAC CCCACAACCA GCCCTATGAG 
CTGTACGACC CCGAGGAACC AACCTCAGAC AGGACACCTC CTTCCTTCGG GGGTCGGCTT 
AACAACAGCA AACAGGGTTT TATCGGTGCT GGGCGGAGGG CCAAGGAGGA CCAGGCGTTG 
CTATCTGTGC GGCCCCTGCA GGCTCATGAG CTGAACGACT TTCACGGTGT GGCCCCCCTC 
CACTTGCCGC ATATCTGTAG CATCTGTGAC AAGAAGGTGT TTGATTTGAA GGACTGGGAG 
CTGCATGTGA AAGGGAAGCT GCACGCTCAG AAATGCCTGG TCTTCTCTGA AAATGCTGGC 
ATCCGGTGTA TACTTGGTTC GGCAGAGGGA ACATTGTGTG CTTCTCCCAA CAGCACAGCT 
GTTTATAACC CTGCTGGGAA TGAAGATTAT GCCTCAAATC TTGGAACATC ATACGTGCCC 
ATTCCAGCAA GGTCATTCAC TCAGTCAAGC CCCACATTTC CTTTGGCTTC TGTGGGGACA 
ACTTTTGCAC AGCGGAAAGG GGCTGGCCGT GTGGTGCACA TCTGCAATCT CCCTGAAGGA 
AGCTGCACTG AGAATGACGT CATTAACCTG GGGCTGCCCT TTGGAAAGGT CACTAATTAC 
ATCCTCATGA AGTCGACTAA TCAGGCCTTT TTAGAGATGG CTTACACAGA AGCTGCACAG 
GCCATGGTCC AGTATTATCA AGAAAAATCT GCTGTGATCA ATGGTGAGAA GTTGCTCATT 
CGGATGTCCA AGAGATACAA GGAATTGCAG CTCAAGAAAC CCGGGAAGGC CGTGGCTGCC 
ATCATCCAGG ACATCCATTC CCAGAGGGAG AGGGACATGT TCCGGGAAGC AGACAGATAT 
GGCCCAGAAA GGCCGCGGTC TCGTAGTCCG GTGAGCCGGT CACTCTCCCC GAGGTCCCAC 
ACTCCCAGCT TCACCTCCTG CAGCTCTTCC CACAGCCCTC CGGGCCCCTC CCGGGCTGAC 
TGGGGCAATG GCCGGGACTC CTGGGAGCAC TCTCCCTATG CCAGGAGGGA GGAAGAGCGA 
GACCCGGCTC CCTGGAGGGA CAACGGAGAT GACAAGAGGG ACAGGATGGA CCCCTGGGCA 
CATGATCGCA AACACCACCC CCGGCAACTG GACAAGGCTG AGTTGGACGA GCGACCAGAA 
GGAGGGAGGC CCCACCGGGA GAAGTACCCG AGATCTGGGT CTCCCAACCT GCCCCACTCT 
GTGTCCAGCT ACAAAAGCCG TGAAGACGGC TACTACCGGA AAGAGCCCAA AGCCAAGTCG 
GACAAGTATC TGAAGCAGCA GCAGGATGCC CCCGGGAGGT CCAGGAGGAA AGACGAGGCC 
AGGCTGCGGG AAAGCAGACA CCCCCATCCG GATGACTCAG GCAAGGAAGA TGGGCTGGGG 
CCAAAGGTCA CTAGGGCCCC TGAGGGCGCC AAGGCCAAGC AGAATGAGAA AAATAAAACC 
AAGAGAACTG ATAGAGACCA AGAAGGAGCT GATGATAGAA AAGAAAACAC AATGGCAGAG 
AATGAGGCTG GAAAAGAGGA ACAGGAGGGC ATGGAAGAAA GCCCTCAATC AGTGGGCAGA 
CAGGAGAAAG AAGCAGAGTT CTCTGATCCG GAAAACACAA GGACAAAGAA GGAACAAGAT 
TGGGAGAGTG AAAGTGAGGC AGAGGGGGAG AGCTGGTATC CCACTAACAT GGAGGAGCTG 
GTGACAGTGG ACGAGGTTGG GGAAGAAGAA GATTTTATCG TGGAACCAGA CATCCCAGAG 
CTGGAAGAAA TTGTGCCCAT TGACCAGAAA GACAAAATTT GCCCAGAAAC ATGTCTGTGT 
GTGACAACCA CCTTAGACTT AGACCTGGCC CAGGATTTCC CCAAGGAAGG AGTCAAGGCC 
GTAGGGAATG GGGCTGCAGA AATCAGCCTC AAGTCACCCA GAGAACTGCC CTCTGCTTCC 
ACAAGCTGTC CCAGTGACAT GGACGTGGAA ATGCCTGGCC TAAATCTGGA TGCTGAGCGG 
AAGCCAGCTG AAAGTGAGAC AGGCCTCTCC CTGGAGGATT CAGATTGCTA CGAGAAGGAG 
GCAAAGGGAG TGGAGAGCTC AGATGTTCAT CCAGCCCCTA CAGTCCAGCA AATGTCTTCC 
CCTAAGCCAG CAGAGGAGAG GGCCCGGCAG CCAAGCCCAT TTGTGGATGA TTGCAAGACC 
AGGGGGACCC CCGAAGATGG GGCTTGTGAA GGCAGCCCCC TGGAGGAGAA AGCCAGCCCC 
CCCATCGAAA CTGACCTCCA AAACCAAGCT TGCCAAGAAG TGTTGACCCC GGAAAACTCC 
AGGTACGTGG AAATGAAATC TCTGGAGGTG AGGTCACCAG AGTACACTGA AGTGGAACTG 
AAACAGCCCC TTTCTTTGCC CTCTTGGGAA CCAGAGGATG TGTTCAGTGA ACTTAGCATT 
CCTCTAGGGG TGGAGTTCGT GGTTCCCAGG ACTGGCTTTT ATTGCAAGCT GTGTGGGCTG 
TTCTACACGA GCGAGGAGAC AGCAAAGATG AGCCACTGCC GCAGCGCTGT CCACTACAGG 
AACTTACAGA AATATTTGTC CCAGCTGGCC GAGGAGGGCC TCAAGGAGAC CGAGGGGGCA 
GATAGCCCGA GGCCAGAGGA CAGCGGAATC GTGCCACGCT TCGAAAGGAA AAAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu91314
Clone ID Related Accession (Same CDS sequence) XM_017016103.2 , XM_017016103.1
Accession Version XM_017016103.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product RNA-binding protein 20 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016103.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGTCCGCGT CCACAGCTGT GAACCGTGCC GCCAAGCTCC TGGACAAGAA CCCATTCTCG 
GTCAGTAACC CGAACCCTCT GCTTCCTTCA CCTGCCAGTC TCCAGCTGGC TCAACTGCAG 
GCCCAGCTCA CCCTCCACCG GCTGAAGCTG GCACAGACAG CTGTCACCAA CAACACTGCA 
GCCGCCACAG TCCTGAACCA AGTCCTCTCC AAAGTGGCCA TGTCCCAGCC TCTCTTCAAT 
CAACTGAGGC ATCCGTCTGT GATCACTGGC CCCCACGGCC ATGCTGGGGT TCCCCAACAT 
GCTGCAGCCA TACCCAGTAC CCGGTTTCCC TCTAATGCAA TTGCCTTTTC ACCCCCCAGC 
CAGACACGAG GCCCCGGACC CTCCATGAAC CTTCCCAACC AGCCACCCAG TGCCATGGTG 
ATGCATCCTT TCACTGGGGT AATGCCTCAG ACCCCTGGCC AGCCAGCAGT CATCTTGGGC 
ATTGGCAAGA CTGGGCCTGC TCCAGCTACA GCAGGATTCT ATGAGTATGG CAAAGCCAGC 
TCTGGCCAGA CATATGGCCC TGAAACAGAT GGTCAGCCTG GCTTCCTGCC ATCCTCGGCC 
TCAACCTCGG GCAGTGTGAC CTATGAAGGG CACTACAGCC ACACAGGGCA GGATGGTCAA 
GCTGCCTTTT CCAAAGATTT TTACGGACCC AACTCCCAAG GTTCACATGT GGCCAGCGGA 
TTTCCAGCTG AGCAGGCTGG GGGCCTGAAA AGTGAGGTCG GGCCACTGCT GCAGGGCACA 
AACAGCCAAT GGGAGAGCCC CCATGGATTC TCGGGCCAAA GCAAGCCTGA TCTCACAGCA 
GGTCCCATGT GGCCTCCACC CCACAACCAG CCCTATGAGC TGTACGACCC CGAGGAACCA 
ACCTCAGACA GGACACCTCC TTCCTTCGGG GGTCGGCTTA ACAACAGCAA ACAGGGTTTT 
ATCGGTGCTG GGCGGAGGGC CAAGGAGGAC CAGGCGTTGC TATCTGTGCG GCCCCTGCAG 
GCTCATGAGC TGAACGACTT TCACGGTGTG GCCCCCCTCC ACTTGCCGCA TATCTGTAGC 
ATCTGTGACA AGAAGGTGTT TGATTTGAAG GACTGGGAGC TGCATGTGAA AGGGAAGCTG 
CACGCTCAGA AATGCCTGGT CTTCTCTGAA AATGCTGGCA TCCGGTGTAT ACTTGGTTCG 
GCAGAGGGAA CATTGTGTGC TTCTCCCAAC AGCACAGCTG TTTATAACCC TGCTGGGAAT 
GAAGATTATG CCTCAAATCT TGGAACATCA TACGTGCCCA TTCCAGCAAG GTCATTCACT 
CAGTCAAGCC CCACATTTCC TTTGGCTTCT GTGGGGACAA CTTTTGCACA GCGGAAAGGG 
GCTGGCCGTG TGGTGCACAT CTGCAATCTC CCTGAAGGAA GCTGCACTGA GAATGACGTC 
ATTAACCTGG GGCTGCCCTT TGGAAAGGTC ACTAATTACA TCCTCATGAA GTCGACTAAT 
CAGGCCTTTT TAGAGATGGC TTACACAGAA GCTGCACAGG CCATGGTCCA GTATTATCAA 
GAAAAATCTG CTGTGATCAA TGGTGAGAAG TTGCTCATTC GGATGTCCAA GAGATACAAG 
GAATTGCAGC TCAAGAAACC CGGGAAGGCC GTGGCTGCCA TCATCCAGGA CATCCATTCC 
CAGAGGGAGA GGGACATGTT CCGGGAAGCA GACAGATATG GCCCAGAAAG GCCGCGGTCT 
CGTAGTCCGG TGAGCCGGTC ACTCTCCCCG AGGTCCCACA CTCCCAGCTT CACCTCCTGC 
AGCTCTTCCC ACAGCCCTCC GGGCCCCTCC CGGGCTGACT GGGGCAATGG CCGGGACTCC 
TGGGAGCACT CTCCCTATGC CAGGAGGGAG GAAGAGCGAG ACCCGGCTCC CTGGAGGGAC 
AACGGAGATG ACAAGAGGGA CAGGATGGAC CCCTGGGCAC ATGATCGCAA ACACCACCCC 
CGGCAACTGG ACAAGGCTGA GTTGGACGAG CGACCAGAAG GAGGGAGGCC CCACCGGGAG 
AAGTACCCGA GATCTGGGTC TCCCAACCTG CCCCACTCTG TGTCCAGCTA CAAAAGCCGT 
GAAGACGGCT ACTACCGGAA AGAGCCCAAA GCCAAGTCGG ACAAGTATCT GAAGCAGCAG 
CAGGATGCCC CCGGGAGGTC CAGGAGGAAA GACGAGGCCA GGCTGCGGGA AAGCAGACAC 
CCCCATCCGG ATGACTCAGG CAAGGAAGAT GGGCTGGGGC CAAAGGTCAC TAGGGCCCCT 
GAGGGCGCCA AGGCCAAGCA GAATGAGAAA AATAAAACCA AGAGAACTGA TAGAGACCAA 
GAAGGAGCTG ATGATAGAAA AGAAAACACA ATGGCAGAGA ATGAGGCTGG AAAAGAGGAA 
CAGGAGGGCA TGGAAGAAAG CCCTCAATCA GTGGGCAGAC AGGAGAAAGA AGCAGAGTTC 
TCTGATCCGG AAAACACAAG GACAAAGAAG GAACAAGATT GGGAGAGTGA AAGTGAGGCA 
GAGGGGGAGA GCTGGTATCC CACTAACATG GAGGAGCTGG TGACAGTGGA CGAGGTTGGG 
GAAGAAGAAG ATTTTATCGT GGAACCAGAC ATCCCAGAGC TGGAAGAAAT TGTGCCCATT 
GACCAGAAAG ACAAAATTTG CCCAGAAACA TGTCTGTGTG TGACAACCAC CTTAGACTTA 
GACCTGGCCC AGGATTTCCC CAAGGAAGGA GTCAAGGCCG TAGGGAATGG GGCTGCAGAA 
ATCAGCCTCA AGTCACCCAG AGAACTGCCC TCTGCTTCCA CAAGCTGTCC CAGTGACATG 
GACGTGGAAA TGCCTGGCCT AAATCTGGAT GCTGAGCGGA AGCCAGCTGA AAGTGAGACA 
GGCCTCTCCC TGGAGGATTC AGATTGCTAC GAGAAGGAGG CAAAGGGAGT GGAGAGCTCA 
GATGTTCATC CAGCCCCTAC AGTCCAGCAA ATGTCTTCCC CTAAGCCAGC AGAGGAGAGG 
GCCCGGCAGC CAAGCCCATT TGTGGATGAT TGCAAGACCA GGGGGACCCC CGAAGATGGG 
GCTTGTGAAG GCAGCCCCCT GGAGGAGAAA GCCAGCCCCC CCATCGAAAC TGACCTCCAA 
AACCAAGCTT GCCAAGAAGT GTTGACCCCG GAAAACTCCA GGTACGTGGA AATGAAATCT 
CTGGAGGTGA GGTCACCAGA GTACACTGAA GTGGAACTGA AACAGCCCCT TTCTTTGCCC 
TCTTGGGAAC CAGAGGATGT GTTCAGTGAA CTTAGCATTC CTCTAGGGGT GGAGTTCGTG 
GTTCCCAGGA CTGGCTTTTA TTGCAAGCTG TGTGGGCTGT TCTACACGAG CGAGGAGACA 
GCAAAGATGA GCCACTGCCG CAGCGCTGTC CACTACAGGA ACTTACAGAA ATATTTGTCC 
CAGCTGGCCG AGGAGGGCCT CAAGGAGACC GAGGGGGCAG ATAGCCCGAG GCCAGAGGAC 
AGCGGAATCG TGCCACGCTT CGAAAGGAAA AAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016871592.1
CDS1483..5001
Translation

Target ORF information:

RefSeq Version XM_017016103.2
Organism Homo sapiens(human)
Definition Homo sapiens RNA binding motif protein 20 (RBM20), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017016103.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGTCCGCGT CCACAGCTGT GAACCGTGCC GCCAAGCTCC TGGACAAGAA CCCATTCTCG 
GTCAGTAACC CGAACCCTCT GCTTCCTTCA CCTGCCAGTC TCCAGCTGGC TCAACTGCAG 
GCCCAGCTCA CCCTCCACCG GCTGAAGCTG GCACAGACAG CTGTCACCAA CAACACTGCA 
GCCGCCACAG TCCTGAACCA AGTCCTCTCC AAAGTGGCCA TGTCCCAGCC TCTCTTCAAT 
CAACTGAGGC ATCCGTCTGT GATCACTGGC CCCCACGGCC ATGCTGGGGT TCCCCAACAT 
GCTGCAGCCA TACCCAGTAC CCGGTTTCCC TCTAATGCAA TTGCCTTTTC ACCCCCCAGC 
CAGACACGAG GCCCCGGACC CTCCATGAAC CTTCCCAACC AGCCACCCAG TGCCATGGTG 
ATGCATCCTT TCACTGGGGT AATGCCTCAG ACCCCTGGCC AGCCAGCAGT CATCTTGGGC 
ATTGGCAAGA CTGGGCCTGC TCCAGCTACA GCAGGATTCT ATGAGTATGG CAAAGCCAGC 
TCTGGCCAGA CATATGGCCC TGAAACAGAT GGTCAGCCTG GCTTCCTGCC ATCCTCGGCC 
TCAACCTCGG GCAGTGTGAC CTATGAAGGG CACTACAGCC ACACAGGGCA GGATGGTCAA 
GCTGCCTTTT CCAAAGATTT TTACGGACCC AACTCCCAAG GTTCACATGT GGCCAGCGGA 
TTTCCAGCTG AGCAGGCTGG GGGCCTGAAA AGTGAGGTCG GGCCACTGCT GCAGGGCACA 
AACAGCCAAT GGGAGAGCCC CCATGGATTC TCGGGCCAAA GCAAGCCTGA TCTCACAGCA 
GGTCCCATGT GGCCTCCACC CCACAACCAG CCCTATGAGC TGTACGACCC CGAGGAACCA 
ACCTCAGACA GGACACCTCC TTCCTTCGGG GGTCGGCTTA ACAACAGCAA ACAGGGTTTT 
ATCGGTGCTG GGCGGAGGGC CAAGGAGGAC CAGGCGTTGC TATCTGTGCG GCCCCTGCAG 
GCTCATGAGC TGAACGACTT TCACGGTGTG GCCCCCCTCC ACTTGCCGCA TATCTGTAGC 
ATCTGTGACA AGAAGGTGTT TGATTTGAAG GACTGGGAGC TGCATGTGAA AGGGAAGCTG 
CACGCTCAGA AATGCCTGGT CTTCTCTGAA AATGCTGGCA TCCGGTGTAT ACTTGGTTCG 
GCAGAGGGAA CATTGTGTGC TTCTCCCAAC AGCACAGCTG TTTATAACCC TGCTGGGAAT 
GAAGATTATG CCTCAAATCT TGGAACATCA TACGTGCCCA TTCCAGCAAG GTCATTCACT 
CAGTCAAGCC CCACATTTCC TTTGGCTTCT GTGGGGACAA CTTTTGCACA GCGGAAAGGG 
GCTGGCCGTG TGGTGCACAT CTGCAATCTC CCTGAAGGAA GCTGCACTGA GAATGACGTC 
ATTAACCTGG GGCTGCCCTT TGGAAAGGTC ACTAATTACA TCCTCATGAA GTCGACTAAT 
CAGGCCTTTT TAGAGATGGC TTACACAGAA GCTGCACAGG CCATGGTCCA GTATTATCAA 
GAAAAATCTG CTGTGATCAA TGGTGAGAAG TTGCTCATTC GGATGTCCAA GAGATACAAG 
GAATTGCAGC TCAAGAAACC CGGGAAGGCC GTGGCTGCCA TCATCCAGGA CATCCATTCC 
CAGAGGGAGA GGGACATGTT CCGGGAAGCA GACAGATATG GCCCAGAAAG GCCGCGGTCT 
CGTAGTCCGG TGAGCCGGTC ACTCTCCCCG AGGTCCCACA CTCCCAGCTT CACCTCCTGC 
AGCTCTTCCC ACAGCCCTCC GGGCCCCTCC CGGGCTGACT GGGGCAATGG CCGGGACTCC 
TGGGAGCACT CTCCCTATGC CAGGAGGGAG GAAGAGCGAG ACCCGGCTCC CTGGAGGGAC 
AACGGAGATG ACAAGAGGGA CAGGATGGAC CCCTGGGCAC ATGATCGCAA ACACCACCCC 
CGGCAACTGG ACAAGGCTGA GTTGGACGAG CGACCAGAAG GAGGGAGGCC CCACCGGGAG 
AAGTACCCGA GATCTGGGTC TCCCAACCTG CCCCACTCTG TGTCCAGCTA CAAAAGCCGT 
GAAGACGGCT ACTACCGGAA AGAGCCCAAA GCCAAGTCGG ACAAGTATCT GAAGCAGCAG 
CAGGATGCCC CCGGGAGGTC CAGGAGGAAA GACGAGGCCA GGCTGCGGGA AAGCAGACAC 
CCCCATCCGG ATGACTCAGG CAAGGAAGAT GGGCTGGGGC CAAAGGTCAC TAGGGCCCCT 
GAGGGCGCCA AGGCCAAGCA GAATGAGAAA AATAAAACCA AGAGAACTGA TAGAGACCAA 
GAAGGAGCTG ATGATAGAAA AGAAAACACA ATGGCAGAGA ATGAGGCTGG AAAAGAGGAA 
CAGGAGGGCA TGGAAGAAAG CCCTCAATCA GTGGGCAGAC AGGAGAAAGA AGCAGAGTTC 
TCTGATCCGG AAAACACAAG GACAAAGAAG GAACAAGATT GGGAGAGTGA AAGTGAGGCA 
GAGGGGGAGA GCTGGTATCC CACTAACATG GAGGAGCTGG TGACAGTGGA CGAGGTTGGG 
GAAGAAGAAG ATTTTATCGT GGAACCAGAC ATCCCAGAGC TGGAAGAAAT TGTGCCCATT 
GACCAGAAAG ACAAAATTTG CCCAGAAACA TGTCTGTGTG TGACAACCAC CTTAGACTTA 
GACCTGGCCC AGGATTTCCC CAAGGAAGGA GTCAAGGCCG TAGGGAATGG GGCTGCAGAA 
ATCAGCCTCA AGTCACCCAG AGAACTGCCC TCTGCTTCCA CAAGCTGTCC CAGTGACATG 
GACGTGGAAA TGCCTGGCCT AAATCTGGAT GCTGAGCGGA AGCCAGCTGA AAGTGAGACA 
GGCCTCTCCC TGGAGGATTC AGATTGCTAC GAGAAGGAGG CAAAGGGAGT GGAGAGCTCA 
GATGTTCATC CAGCCCCTAC AGTCCAGCAA ATGTCTTCCC CTAAGCCAGC AGAGGAGAGG 
GCCCGGCAGC CAAGCCCATT TGTGGATGAT TGCAAGACCA GGGGGACCCC CGAAGATGGG 
GCTTGTGAAG GCAGCCCCCT GGAGGAGAAA GCCAGCCCCC CCATCGAAAC TGACCTCCAA 
AACCAAGCTT GCCAAGAAGT GTTGACCCCG GAAAACTCCA GGTACGTGGA AATGAAATCT 
CTGGAGGTGA GGTCACCAGA GTACACTGAA GTGGAACTGA AACAGCCCCT TTCTTTGCCC 
TCTTGGGAAC CAGAGGATGT GTTCAGTGAA CTTAGCATTC CTCTAGGGGT GGAGTTCGTG 
GTTCCCAGGA CTGGCTTTTA TTGCAAGCTG TGTGGGCTGT TCTACACGAG CGAGGAGACA 
GCAAAGATGA GCCACTGCCG CAGCGCTGTC CACTACAGGA ACTTACAGAA ATATTTGTCC 
CAGCTGGCCG AGGAGGGCCT CAAGGAGACC GAGGGGGCAG ATAGCCCGAG GCCAGAGGAC 
AGCGGAATCG TGCCACGCTT CGAAAGGAAA AAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu91314
Clone ID Related Accession (Same CDS sequence) XM_017016103.2 , XM_017016103.1
Accession Version XM_017016103.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product RNA-binding protein 20 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGTCCGCGT CCACAGCTGT GAACCGTGCC GCCAAGCTCC TGGACAAGAA CCCATTCTCG 
GTCAGTAACC CGAACCCTCT GCTTCCTTCA CCTGCCAGTC TCCAGCTGGC TCAACTGCAG 
GCCCAGCTCA CCCTCCACCG GCTGAAGCTG GCACAGACAG CTGTCACCAA CAACACTGCA 
GCCGCCACAG TCCTGAACCA AGTCCTCTCC AAAGTGGCCA TGTCCCAGCC TCTCTTCAAT 
CAACTGAGGC ATCCGTCTGT GATCACTGGC CCCCACGGCC ATGCTGGGGT TCCCCAACAT 
GCTGCAGCCA TACCCAGTAC CCGGTTTCCC TCTAATGCAA TTGCCTTTTC ACCCCCCAGC 
CAGACACGAG GCCCCGGACC CTCCATGAAC CTTCCCAACC AGCCACCCAG TGCCATGGTG 
ATGCATCCTT TCACTGGGGT AATGCCTCAG ACCCCTGGCC AGCCAGCAGT CATCTTGGGC 
ATTGGCAAGA CTGGGCCTGC TCCAGCTACA GCAGGATTCT ATGAGTATGG CAAAGCCAGC 
TCTGGCCAGA CATATGGCCC TGAAACAGAT GGTCAGCCTG GCTTCCTGCC ATCCTCGGCC 
TCAACCTCGG GCAGTGTGAC CTATGAAGGG CACTACAGCC ACACAGGGCA GGATGGTCAA 
GCTGCCTTTT CCAAAGATTT TTACGGACCC AACTCCCAAG GTTCACATGT GGCCAGCGGA 
TTTCCAGCTG AGCAGGCTGG GGGCCTGAAA AGTGAGGTCG GGCCACTGCT GCAGGGCACA 
AACAGCCAAT GGGAGAGCCC CCATGGATTC TCGGGCCAAA GCAAGCCTGA TCTCACAGCA 
GGTCCCATGT GGCCTCCACC CCACAACCAG CCCTATGAGC TGTACGACCC CGAGGAACCA 
ACCTCAGACA GGACACCTCC TTCCTTCGGG GGTCGGCTTA ACAACAGCAA ACAGGGTTTT 
ATCGGTGCTG GGCGGAGGGC CAAGGAGGAC CAGGCGTTGC TATCTGTGCG GCCCCTGCAG 
GCTCATGAGC TGAACGACTT TCACGGTGTG GCCCCCCTCC ACTTGCCGCA TATCTGTAGC 
ATCTGTGACA AGAAGGTGTT TGATTTGAAG GACTGGGAGC TGCATGTGAA AGGGAAGCTG 
CACGCTCAGA AATGCCTGGT CTTCTCTGAA AATGCTGGCA TCCGGTGTAT ACTTGGTTCG 
GCAGAGGGAA CATTGTGTGC TTCTCCCAAC AGCACAGCTG TTTATAACCC TGCTGGGAAT 
GAAGATTATG CCTCAAATCT TGGAACATCA TACGTGCCCA TTCCAGCAAG GTCATTCACT 
CAGTCAAGCC CCACATTTCC TTTGGCTTCT GTGGGGACAA CTTTTGCACA GCGGAAAGGG 
GCTGGCCGTG TGGTGCACAT CTGCAATCTC CCTGAAGGAA GCTGCACTGA GAATGACGTC 
ATTAACCTGG GGCTGCCCTT TGGAAAGGTC ACTAATTACA TCCTCATGAA GTCGACTAAT 
CAGGCCTTTT TAGAGATGGC TTACACAGAA GCTGCACAGG CCATGGTCCA GTATTATCAA 
GAAAAATCTG CTGTGATCAA TGGTGAGAAG TTGCTCATTC GGATGTCCAA GAGATACAAG 
GAATTGCAGC TCAAGAAACC CGGGAAGGCC GTGGCTGCCA TCATCCAGGA CATCCATTCC 
CAGAGGGAGA GGGACATGTT CCGGGAAGCA GACAGATATG GCCCAGAAAG GCCGCGGTCT 
CGTAGTCCGG TGAGCCGGTC ACTCTCCCCG AGGTCCCACA CTCCCAGCTT CACCTCCTGC 
AGCTCTTCCC ACAGCCCTCC GGGCCCCTCC CGGGCTGACT GGGGCAATGG CCGGGACTCC 
TGGGAGCACT CTCCCTATGC CAGGAGGGAG GAAGAGCGAG ACCCGGCTCC CTGGAGGGAC 
AACGGAGATG ACAAGAGGGA CAGGATGGAC CCCTGGGCAC ATGATCGCAA ACACCACCCC 
CGGCAACTGG ACAAGGCTGA GTTGGACGAG CGACCAGAAG GAGGGAGGCC CCACCGGGAG 
AAGTACCCGA GATCTGGGTC TCCCAACCTG CCCCACTCTG TGTCCAGCTA CAAAAGCCGT 
GAAGACGGCT ACTACCGGAA AGAGCCCAAA GCCAAGTCGG ACAAGTATCT GAAGCAGCAG 
CAGGATGCCC CCGGGAGGTC CAGGAGGAAA GACGAGGCCA GGCTGCGGGA AAGCAGACAC 
CCCCATCCGG ATGACTCAGG CAAGGAAGAT GGGCTGGGGC CAAAGGTCAC TAGGGCCCCT 
GAGGGCGCCA AGGCCAAGCA GAATGAGAAA AATAAAACCA AGAGAACTGA TAGAGACCAA 
GAAGGAGCTG ATGATAGAAA AGAAAACACA ATGGCAGAGA ATGAGGCTGG AAAAGAGGAA 
CAGGAGGGCA TGGAAGAAAG CCCTCAATCA GTGGGCAGAC AGGAGAAAGA AGCAGAGTTC 
TCTGATCCGG AAAACACAAG GACAAAGAAG GAACAAGATT GGGAGAGTGA AAGTGAGGCA 
GAGGGGGAGA GCTGGTATCC CACTAACATG GAGGAGCTGG TGACAGTGGA CGAGGTTGGG 
GAAGAAGAAG ATTTTATCGT GGAACCAGAC ATCCCAGAGC TGGAAGAAAT TGTGCCCATT 
GACCAGAAAG ACAAAATTTG CCCAGAAACA TGTCTGTGTG TGACAACCAC CTTAGACTTA 
GACCTGGCCC AGGATTTCCC CAAGGAAGGA GTCAAGGCCG TAGGGAATGG GGCTGCAGAA 
ATCAGCCTCA AGTCACCCAG AGAACTGCCC TCTGCTTCCA CAAGCTGTCC CAGTGACATG 
GACGTGGAAA TGCCTGGCCT AAATCTGGAT GCTGAGCGGA AGCCAGCTGA AAGTGAGACA 
GGCCTCTCCC TGGAGGATTC AGATTGCTAC GAGAAGGAGG CAAAGGGAGT GGAGAGCTCA 
GATGTTCATC CAGCCCCTAC AGTCCAGCAA ATGTCTTCCC CTAAGCCAGC AGAGGAGAGG 
GCCCGGCAGC CAAGCCCATT TGTGGATGAT TGCAAGACCA GGGGGACCCC CGAAGATGGG 
GCTTGTGAAG GCAGCCCCCT GGAGGAGAAA GCCAGCCCCC CCATCGAAAC TGACCTCCAA 
AACCAAGCTT GCCAAGAAGT GTTGACCCCG GAAAACTCCA GGTACGTGGA AATGAAATCT 
CTGGAGGTGA GGTCACCAGA GTACACTGAA GTGGAACTGA AACAGCCCCT TTCTTTGCCC 
TCTTGGGAAC CAGAGGATGT GTTCAGTGAA CTTAGCATTC CTCTAGGGGT GGAGTTCGTG 
GTTCCCAGGA CTGGCTTTTA TTGCAAGCTG TGTGGGCTGT TCTACACGAG CGAGGAGACA 
GCAAAGATGA GCCACTGCCG CAGCGCTGTC CACTACAGGA ACTTACAGAA ATATTTGTCC 
CAGCTGGCCG AGGAGGGCCT CAAGGAGACC GAGGGGGCAG ATAGCCCGAG GCCAGAGGAC 
AGCGGAATCG TGCCACGCTT CGAAAGGAAA AAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016871592.1
CDS1482..5000
Translation

Target ORF information:

RefSeq Version XM_017016103.1
Organism Homo sapiens(human)
Definition Homo sapiens RNA binding motif protein 20 (RBM20), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017016103.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
ATGTCCGCGT CCACAGCTGT GAACCGTGCC GCCAAGCTCC TGGACAAGAA CCCATTCTCG 
GTCAGTAACC CGAACCCTCT GCTTCCTTCA CCTGCCAGTC TCCAGCTGGC TCAACTGCAG 
GCCCAGCTCA CCCTCCACCG GCTGAAGCTG GCACAGACAG CTGTCACCAA CAACACTGCA 
GCCGCCACAG TCCTGAACCA AGTCCTCTCC AAAGTGGCCA TGTCCCAGCC TCTCTTCAAT 
CAACTGAGGC ATCCGTCTGT GATCACTGGC CCCCACGGCC ATGCTGGGGT TCCCCAACAT 
GCTGCAGCCA TACCCAGTAC CCGGTTTCCC TCTAATGCAA TTGCCTTTTC ACCCCCCAGC 
CAGACACGAG GCCCCGGACC CTCCATGAAC CTTCCCAACC AGCCACCCAG TGCCATGGTG 
ATGCATCCTT TCACTGGGGT AATGCCTCAG ACCCCTGGCC AGCCAGCAGT CATCTTGGGC 
ATTGGCAAGA CTGGGCCTGC TCCAGCTACA GCAGGATTCT ATGAGTATGG CAAAGCCAGC 
TCTGGCCAGA CATATGGCCC TGAAACAGAT GGTCAGCCTG GCTTCCTGCC ATCCTCGGCC 
TCAACCTCGG GCAGTGTGAC CTATGAAGGG CACTACAGCC ACACAGGGCA GGATGGTCAA 
GCTGCCTTTT CCAAAGATTT TTACGGACCC AACTCCCAAG GTTCACATGT GGCCAGCGGA 
TTTCCAGCTG AGCAGGCTGG GGGCCTGAAA AGTGAGGTCG GGCCACTGCT GCAGGGCACA 
AACAGCCAAT GGGAGAGCCC CCATGGATTC TCGGGCCAAA GCAAGCCTGA TCTCACAGCA 
GGTCCCATGT GGCCTCCACC CCACAACCAG CCCTATGAGC TGTACGACCC CGAGGAACCA 
ACCTCAGACA GGACACCTCC TTCCTTCGGG GGTCGGCTTA ACAACAGCAA ACAGGGTTTT 
ATCGGTGCTG GGCGGAGGGC CAAGGAGGAC CAGGCGTTGC TATCTGTGCG GCCCCTGCAG 
GCTCATGAGC TGAACGACTT TCACGGTGTG GCCCCCCTCC ACTTGCCGCA TATCTGTAGC 
ATCTGTGACA AGAAGGTGTT TGATTTGAAG GACTGGGAGC TGCATGTGAA AGGGAAGCTG 
CACGCTCAGA AATGCCTGGT CTTCTCTGAA AATGCTGGCA TCCGGTGTAT ACTTGGTTCG 
GCAGAGGGAA CATTGTGTGC TTCTCCCAAC AGCACAGCTG TTTATAACCC TGCTGGGAAT 
GAAGATTATG CCTCAAATCT TGGAACATCA TACGTGCCCA TTCCAGCAAG GTCATTCACT 
CAGTCAAGCC CCACATTTCC TTTGGCTTCT GTGGGGACAA CTTTTGCACA GCGGAAAGGG 
GCTGGCCGTG TGGTGCACAT CTGCAATCTC CCTGAAGGAA GCTGCACTGA GAATGACGTC 
ATTAACCTGG GGCTGCCCTT TGGAAAGGTC ACTAATTACA TCCTCATGAA GTCGACTAAT 
CAGGCCTTTT TAGAGATGGC TTACACAGAA GCTGCACAGG CCATGGTCCA GTATTATCAA 
GAAAAATCTG CTGTGATCAA TGGTGAGAAG TTGCTCATTC GGATGTCCAA GAGATACAAG 
GAATTGCAGC TCAAGAAACC CGGGAAGGCC GTGGCTGCCA TCATCCAGGA CATCCATTCC 
CAGAGGGAGA GGGACATGTT CCGGGAAGCA GACAGATATG GCCCAGAAAG GCCGCGGTCT 
CGTAGTCCGG TGAGCCGGTC ACTCTCCCCG AGGTCCCACA CTCCCAGCTT CACCTCCTGC 
AGCTCTTCCC ACAGCCCTCC GGGCCCCTCC CGGGCTGACT GGGGCAATGG CCGGGACTCC 
TGGGAGCACT CTCCCTATGC CAGGAGGGAG GAAGAGCGAG ACCCGGCTCC CTGGAGGGAC 
AACGGAGATG ACAAGAGGGA CAGGATGGAC CCCTGGGCAC ATGATCGCAA ACACCACCCC 
CGGCAACTGG ACAAGGCTGA GTTGGACGAG CGACCAGAAG GAGGGAGGCC CCACCGGGAG 
AAGTACCCGA GATCTGGGTC TCCCAACCTG CCCCACTCTG TGTCCAGCTA CAAAAGCCGT 
GAAGACGGCT ACTACCGGAA AGAGCCCAAA GCCAAGTCGG ACAAGTATCT GAAGCAGCAG 
CAGGATGCCC CCGGGAGGTC CAGGAGGAAA GACGAGGCCA GGCTGCGGGA AAGCAGACAC 
CCCCATCCGG ATGACTCAGG CAAGGAAGAT GGGCTGGGGC CAAAGGTCAC TAGGGCCCCT 
GAGGGCGCCA AGGCCAAGCA GAATGAGAAA AATAAAACCA AGAGAACTGA TAGAGACCAA 
GAAGGAGCTG ATGATAGAAA AGAAAACACA ATGGCAGAGA ATGAGGCTGG AAAAGAGGAA 
CAGGAGGGCA TGGAAGAAAG CCCTCAATCA GTGGGCAGAC AGGAGAAAGA AGCAGAGTTC 
TCTGATCCGG AAAACACAAG GACAAAGAAG GAACAAGATT GGGAGAGTGA AAGTGAGGCA 
GAGGGGGAGA GCTGGTATCC CACTAACATG GAGGAGCTGG TGACAGTGGA CGAGGTTGGG 
GAAGAAGAAG ATTTTATCGT GGAACCAGAC ATCCCAGAGC TGGAAGAAAT TGTGCCCATT 
GACCAGAAAG ACAAAATTTG CCCAGAAACA TGTCTGTGTG TGACAACCAC CTTAGACTTA 
GACCTGGCCC AGGATTTCCC CAAGGAAGGA GTCAAGGCCG TAGGGAATGG GGCTGCAGAA 
ATCAGCCTCA AGTCACCCAG AGAACTGCCC TCTGCTTCCA CAAGCTGTCC CAGTGACATG 
GACGTGGAAA TGCCTGGCCT AAATCTGGAT GCTGAGCGGA AGCCAGCTGA AAGTGAGACA 
GGCCTCTCCC TGGAGGATTC AGATTGCTAC GAGAAGGAGG CAAAGGGAGT GGAGAGCTCA 
GATGTTCATC CAGCCCCTAC AGTCCAGCAA ATGTCTTCCC CTAAGCCAGC AGAGGAGAGG 
GCCCGGCAGC CAAGCCCATT TGTGGATGAT TGCAAGACCA GGGGGACCCC CGAAGATGGG 
GCTTGTGAAG GCAGCCCCCT GGAGGAGAAA GCCAGCCCCC CCATCGAAAC TGACCTCCAA 
AACCAAGCTT GCCAAGAAGT GTTGACCCCG GAAAACTCCA GGTACGTGGA AATGAAATCT 
CTGGAGGTGA GGTCACCAGA GTACACTGAA GTGGAACTGA AACAGCCCCT TTCTTTGCCC 
TCTTGGGAAC CAGAGGATGT GTTCAGTGAA CTTAGCATTC CTCTAGGGGT GGAGTTCGTG 
GTTCCCAGGA CTGGCTTTTA TTGCAAGCTG TGTGGGCTGT TCTACACGAG CGAGGAGACA 
GCAAAGATGA GCCACTGCCG CAGCGCTGTC CACTACAGGA ACTTACAGAA ATATTTGTCC 
CAGCTGGCCG AGGAGGGCCT CAAGGAGACC GAGGGGGCAG ATAGCCCGAG GCCAGAGGAC 
AGCGGAATCG TGCCACGCTT CGAAAGGAAA AAGCTCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

RBM20 Regulates Circular RNA Production From the Titin Gene.
Circulation research119(9)996-1003(2016 Oct)
Khan MA,Reckman YJ,Aufiero S,van den Hoogenhof MM,van der Made I,Beqqali A,Koolbergen DR,Rasmussen TB,van der Velden J,Creemers EE,Pinto YM