ANGPT1 cDNA ORF clone, Homo sapiens(Human)

  • Gene

  • Clones

  • gRNAs

The following ANGPT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANGPT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18352 NM_001146.5
Latest version!
Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu06136 NM_001199859.3
Latest version!
Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu102700 NM_001314051.1
Latest version!
Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
View Old Accession Versions >>
OHu18352 NM_001146.4 Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu06136 NM_001199859.1 Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu06136 NM_001199859.2 Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu18352 NM_001146.3 Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu18352
    Clone ID Related Accession (Same CDS sequence) NM_001146.5 , NM_001146.4 , NM_001146.3
    Accession Version NM_001146.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-16
    Organism Homo sapiens(human)
    Product angiopoietin-1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX648814.1, BC152419.1 and AP000428.3. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001146.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648814.1, BC152411.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000517746.6/ ENSP00000428340.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
    AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
    TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
    CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
    CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
    GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
    CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
    CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
    ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
    ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGGTGTT TTACTAAAGG GAGGAAAAAG AGAGGAAGAG
    AAACCATTTA GAGACTGTGC AGATGTATAT CAAGCTGGTT TTAATAAAAG TGGAATCTAC
    ACTATTTATA TTAATAATAT GCCAGAACCC AAAAAGGTGT TTTGCAATAT GGATGTCAAT
    GGGGGAGGTT GGACTGTAAT ACAACATCGT GAAGATGGAA GTCTAGATTT CCAAAGAGGC
    TGGAAGGAAT ATAAAATGGG TTTTGGAAAT CCCTCCGGTG AATATTGGCT GGGGAATGAG
    TTTATTTTTG CCATTACCAG TCAGAGGCAG TACATGCTAA GAATTGAGTT AATGGACTGG
    GAAGGGAACC GAGCCTATTC ACAGTATGAC AGATTCCACA TAGGAAATGA AAAGCAAAAC
    TATAGGTTGT ATTTAAAAGG TCACACTGGG ACAGCAGGAA AACAGAGCAG CCTGATCTTA
    CACGGTGCTG ATTTCAGCAC TAAAGATGCT GATAATGACA ACTGTATGTG CAAATGTGCC
    CTCATGTTAA CAGGAGGATG GTGGTTTGAT GCTTGTGGCC CCTCCAATCT AAATGGAATG
    TTCTATACTG CGGGACAAAA CCATGGAAAA CTGAATGGGA TAAAGTGGCA CTACTTCAAA
    GGGCCCAGTT ACTCCTTACG TTCCACAACT ATGATGATTC GACCTTTAGA TTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137.2
    CDS361..1857
    Translation

    Target ORF information:

    RefSeq Version NM_001146.5
    Organism Homo sapiens(human)
    Definition Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
    AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
    TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
    CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
    CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
    GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
    CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
    CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
    ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
    ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGGTGTT TTACTAAAGG GAGGAAAAAG AGAGGAAGAG
    AAACCATTTA GAGACTGTGC AGATGTATAT CAAGCTGGTT TTAATAAAAG TGGAATCTAC
    ACTATTTATA TTAATAATAT GCCAGAACCC AAAAAGGTGT TTTGCAATAT GGATGTCAAT
    GGGGGAGGTT GGACTGTAAT ACAACATCGT GAAGATGGAA GTCTAGATTT CCAAAGAGGC
    TGGAAGGAAT ATAAAATGGG TTTTGGAAAT CCCTCCGGTG AATATTGGCT GGGGAATGAG
    TTTATTTTTG CCATTACCAG TCAGAGGCAG TACATGCTAA GAATTGAGTT AATGGACTGG
    GAAGGGAACC GAGCCTATTC ACAGTATGAC AGATTCCACA TAGGAAATGA AAAGCAAAAC
    TATAGGTTGT ATTTAAAAGG TCACACTGGG ACAGCAGGAA AACAGAGCAG CCTGATCTTA
    CACGGTGCTG ATTTCAGCAC TAAAGATGCT GATAATGACA ACTGTATGTG CAAATGTGCC
    CTCATGTTAA CAGGAGGATG GTGGTTTGAT GCTTGTGGCC CCTCCAATCT AAATGGAATG
    TTCTATACTG CGGGACAAAA CCATGGAAAA CTGAATGGGA TAAAGTGGCA CTACTTCAAA
    GGGCCCAGTT ACTCCTTACG TTCCACAACT ATGATGATTC GACCTTTAGA TTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18352
    Clone ID Related Accession (Same CDS sequence) NM_001146.5 , NM_001146.4 , NM_001146.3
    Accession Version NM_001146.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product angiopoietin-1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX648814.1, BC152419.1 and AP000428.3. This sequence is a reference standard in the RefSeqGene project. On Sep 29, 2015 this sequence version replaced NM_001146.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648814.1, BC152411.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467148 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
    AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
    TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
    CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
    CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
    GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
    CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
    CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
    ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
    ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGGTGTT TTACTAAAGG GAGGAAAAAG AGAGGAAGAG
    AAACCATTTA GAGACTGTGC AGATGTATAT CAAGCTGGTT TTAATAAAAG TGGAATCTAC
    ACTATTTATA TTAATAATAT GCCAGAACCC AAAAAGGTGT TTTGCAATAT GGATGTCAAT
    GGGGGAGGTT GGACTGTAAT ACAACATCGT GAAGATGGAA GTCTAGATTT CCAAAGAGGC
    TGGAAGGAAT ATAAAATGGG TTTTGGAAAT CCCTCCGGTG AATATTGGCT GGGGAATGAG
    TTTATTTTTG CCATTACCAG TCAGAGGCAG TACATGCTAA GAATTGAGTT AATGGACTGG
    GAAGGGAACC GAGCCTATTC ACAGTATGAC AGATTCCACA TAGGAAATGA AAAGCAAAAC
    TATAGGTTGT ATTTAAAAGG TCACACTGGG ACAGCAGGAA AACAGAGCAG CCTGATCTTA
    CACGGTGCTG ATTTCAGCAC TAAAGATGCT GATAATGACA ACTGTATGTG CAAATGTGCC
    CTCATGTTAA CAGGAGGATG GTGGTTTGAT GCTTGTGGCC CCTCCAATCT AAATGGAATG
    TTCTATACTG CGGGACAAAA CCATGGAAAA CTGAATGGGA TAAAGTGGCA CTACTTCAAA
    GGGCCCAGTT ACTCCTTACG TTCCACAACT ATGATGATTC GACCTTTAGA TTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137.2
    CDS498..1994
    Translation

    Target ORF information:

    RefSeq Version NM_001146.4
    Organism Homo sapiens(human)
    Definition Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
    AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
    TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
    CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
    CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
    GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
    CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
    CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
    ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
    ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGGTGTT TTACTAAAGG GAGGAAAAAG AGAGGAAGAG
    AAACCATTTA GAGACTGTGC AGATGTATAT CAAGCTGGTT TTAATAAAAG TGGAATCTAC
    ACTATTTATA TTAATAATAT GCCAGAACCC AAAAAGGTGT TTTGCAATAT GGATGTCAAT
    GGGGGAGGTT GGACTGTAAT ACAACATCGT GAAGATGGAA GTCTAGATTT CCAAAGAGGC
    TGGAAGGAAT ATAAAATGGG TTTTGGAAAT CCCTCCGGTG AATATTGGCT GGGGAATGAG
    TTTATTTTTG CCATTACCAG TCAGAGGCAG TACATGCTAA GAATTGAGTT AATGGACTGG
    GAAGGGAACC GAGCCTATTC ACAGTATGAC AGATTCCACA TAGGAAATGA AAAGCAAAAC
    TATAGGTTGT ATTTAAAAGG TCACACTGGG ACAGCAGGAA AACAGAGCAG CCTGATCTTA
    CACGGTGCTG ATTTCAGCAC TAAAGATGCT GATAATGACA ACTGTATGTG CAAATGTGCC
    CTCATGTTAA CAGGAGGATG GTGGTTTGAT GCTTGTGGCC CCTCCAATCT AAATGGAATG
    TTCTATACTG CGGGACAAAA CCATGGAAAA CTGAATGGGA TAAAGTGGCA CTACTTCAAA
    GGGCCCAGTT ACTCCTTACG TTCCACAACT ATGATGATTC GACCTTTAGA TTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18352
    Clone ID Related Accession (Same CDS sequence) NM_001146.5 , NM_001146.4 , NM_001146.3
    Accession Version NM_001146.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product angiopoietin-1 isoform 1 precursor
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
    AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
    TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
    CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
    CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
    GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
    CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
    CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
    ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
    ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGGTGTT TTACTAAAGG GAGGAAAAAG AGAGGAAGAG
    AAACCATTTA GAGACTGTGC AGATGTATAT CAAGCTGGTT TTAATAAAAG TGGAATCTAC
    ACTATTTATA TTAATAATAT GCCAGAACCC AAAAAGGTGT TTTGCAATAT GGATGTCAAT
    GGGGGAGGTT GGACTGTAAT ACAACATCGT GAAGATGGAA GTCTAGATTT CCAAAGAGGC
    TGGAAGGAAT ATAAAATGGG TTTTGGAAAT CCCTCCGGTG AATATTGGCT GGGGAATGAG
    TTTATTTTTG CCATTACCAG TCAGAGGCAG TACATGCTAA GAATTGAGTT AATGGACTGG
    GAAGGGAACC GAGCCTATTC ACAGTATGAC AGATTCCACA TAGGAAATGA AAAGCAAAAC
    TATAGGTTGT ATTTAAAAGG TCACACTGGG ACAGCAGGAA AACAGAGCAG CCTGATCTTA
    CACGGTGCTG ATTTCAGCAC TAAAGATGCT GATAATGACA ACTGTATGTG CAAATGTGCC
    CTCATGTTAA CAGGAGGATG GTGGTTTGAT GCTTGTGGCC CCTCCAATCT AAATGGAATG
    TTCTATACTG CGGGACAAAA CCATGGAAAA CTGAATGGGA TAAAGTGGCA CTACTTCAAA
    GGGCCCAGTT ACTCCTTACG TTCCACAACT ATGATGATTC GACCTTTAGA TTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137.2
    Translation

    Target ORF information:

    RefSeq Version NM_001146.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
    AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
    TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
    CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
    CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
    GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
    CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
    CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
    ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
    ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGGTGTT TTACTAAAGG GAGGAAAAAG AGAGGAAGAG
    AAACCATTTA GAGACTGTGC AGATGTATAT CAAGCTGGTT TTAATAAAAG TGGAATCTAC
    ACTATTTATA TTAATAATAT GCCAGAACCC AAAAAGGTGT TTTGCAATAT GGATGTCAAT
    GGGGGAGGTT GGACTGTAAT ACAACATCGT GAAGATGGAA GTCTAGATTT CCAAAGAGGC
    TGGAAGGAAT ATAAAATGGG TTTTGGAAAT CCCTCCGGTG AATATTGGCT GGGGAATGAG
    TTTATTTTTG CCATTACCAG TCAGAGGCAG TACATGCTAA GAATTGAGTT AATGGACTGG
    GAAGGGAACC GAGCCTATTC ACAGTATGAC AGATTCCACA TAGGAAATGA AAAGCAAAAC
    TATAGGTTGT ATTTAAAAGG TCACACTGGG ACAGCAGGAA AACAGAGCAG CCTGATCTTA
    CACGGTGCTG ATTTCAGCAC TAAAGATGCT GATAATGACA ACTGTATGTG CAAATGTGCC
    CTCATGTTAA CAGGAGGATG GTGGTTTGAT GCTTGTGGCC CCTCCAATCT AAATGGAATG
    TTCTATACTG CGGGACAAAA CCATGGAAAA CTGAATGGGA TAAAGTGGCA CTACTTCAAA
    GGGCCCAGTT ACTCCTTACG TTCCACAACT ATGATGATTC GACCTTTAGA TTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06136
    Clone ID Related Accession (Same CDS sequence) NM_001199859.1 , NM_001199859.2 , NM_001199859.3
    Accession Version NM_001199859.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product angiopoietin-1 isoform 2 precursor
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
    AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
    TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
    CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
    CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
    GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
    CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
    CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
    ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
    ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGTTTTA CTAAAGGGAG GAAAAAGAGA GGAAGAGAAA
    CCATTTAGAG ACTGTGCAGA TGTATATCAA GCTGGTTTTA ATAAAAGTGG AATCTACACT
    ATTTATATTA ATAATATGCC AGAACCCAAA AAGGTGTTTT GCAATATGGA TGTCAATGGG
    GGAGGTTGGA CTGTAATACA ACATCGTGAA GATGGAAGTC TAGATTTCCA AAGAGGCTGG
    AAGGAATATA AAATGGGTTT TGGAAATCCC TCCGGTGAAT ATTGGCTGGG GAATGAGTTT
    ATTTTTGCCA TTACCAGTCA GAGGCAGTAC ATGCTAAGAA TTGAGTTAAT GGACTGGGAA
    GGGAACCGAG CCTATTCACA GTATGACAGA TTCCACATAG GAAATGAAAA GCAAAACTAT
    AGGTTGTATT TAAAAGGTCA CACTGGGACA GCAGGAAAAC AGAGCAGCCT GATCTTACAC
    GGTGCTGATT TCAGCACTAA AGATGCTGAT AATGACAACT GTATGTGCAA ATGTGCCCTC
    ATGTTAACAG GAGGATGGTG GTTTGATGCT TGTGGCCCCT CCAATCTAAA TGGAATGTTC
    TATACTGCGG GACAAAACCA TGGAAAACTG AATGGGATAA AGTGGCACTA CTTCAAAGGG
    CCCAGTTACT CCTTACGTTC CACAACTATG ATGATTCGAC CTTTAGATTT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186788.1
    Translation

    Target ORF information:

    RefSeq Version NM_001199859.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199859.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
    AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
    TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
    CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
    CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
    GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
    CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
    CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
    ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
    ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGTTTTA CTAAAGGGAG GAAAAAGAGA GGAAGAGAAA
    CCATTTAGAG ACTGTGCAGA TGTATATCAA GCTGGTTTTA ATAAAAGTGG AATCTACACT
    ATTTATATTA ATAATATGCC AGAACCCAAA AAGGTGTTTT GCAATATGGA TGTCAATGGG
    GGAGGTTGGA CTGTAATACA ACATCGTGAA GATGGAAGTC TAGATTTCCA AAGAGGCTGG
    AAGGAATATA AAATGGGTTT TGGAAATCCC TCCGGTGAAT ATTGGCTGGG GAATGAGTTT
    ATTTTTGCCA TTACCAGTCA GAGGCAGTAC ATGCTAAGAA TTGAGTTAAT GGACTGGGAA
    GGGAACCGAG CCTATTCACA GTATGACAGA TTCCACATAG GAAATGAAAA GCAAAACTAT
    AGGTTGTATT TAAAAGGTCA CACTGGGACA GCAGGAAAAC AGAGCAGCCT GATCTTACAC
    GGTGCTGATT TCAGCACTAA AGATGCTGAT AATGACAACT GTATGTGCAA ATGTGCCCTC
    ATGTTAACAG GAGGATGGTG GTTTGATGCT TGTGGCCCCT CCAATCTAAA TGGAATGTTC
    TATACTGCGG GACAAAACCA TGGAAAACTG AATGGGATAA AGTGGCACTA CTTCAAAGGG
    CCCAGTTACT CCTTACGTTC CACAACTATG ATGATTCGAC CTTTAGATTT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06136
    Clone ID Related Accession (Same CDS sequence) NM_001199859.1 , NM_001199859.2 , NM_001199859.3
    Accession Version NM_001199859.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-03
    Organism Homo sapiens(human)
    Product angiopoietin-1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX648814.1, AB084454.1 and AP000428.3. On Sep 29, 2015 this sequence version replaced NM_001199859.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1, which results in an isoform (2) that is one amino acid shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.228403.1, SRR1803614.256349.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
    AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
    TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
    CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
    CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
    GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
    CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
    CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
    ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
    ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGTTTTA CTAAAGGGAG GAAAAAGAGA GGAAGAGAAA
    CCATTTAGAG ACTGTGCAGA TGTATATCAA GCTGGTTTTA ATAAAAGTGG AATCTACACT
    ATTTATATTA ATAATATGCC AGAACCCAAA AAGGTGTTTT GCAATATGGA TGTCAATGGG
    GGAGGTTGGA CTGTAATACA ACATCGTGAA GATGGAAGTC TAGATTTCCA AAGAGGCTGG
    AAGGAATATA AAATGGGTTT TGGAAATCCC TCCGGTGAAT ATTGGCTGGG GAATGAGTTT
    ATTTTTGCCA TTACCAGTCA GAGGCAGTAC ATGCTAAGAA TTGAGTTAAT GGACTGGGAA
    GGGAACCGAG CCTATTCACA GTATGACAGA TTCCACATAG GAAATGAAAA GCAAAACTAT
    AGGTTGTATT TAAAAGGTCA CACTGGGACA GCAGGAAAAC AGAGCAGCCT GATCTTACAC
    GGTGCTGATT TCAGCACTAA AGATGCTGAT AATGACAACT GTATGTGCAA ATGTGCCCTC
    ATGTTAACAG GAGGATGGTG GTTTGATGCT TGTGGCCCCT CCAATCTAAA TGGAATGTTC
    TATACTGCGG GACAAAACCA TGGAAAACTG AATGGGATAA AGTGGCACTA CTTCAAAGGG
    CCCAGTTACT CCTTACGTTC CACAACTATG ATGATTCGAC CTTTAGATTT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186788.1
    CDS498..1991
    Translation

    Target ORF information:

    RefSeq Version NM_001199859.2
    Organism Homo sapiens(human)
    Definition Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199859.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
    AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
    TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
    CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
    CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
    GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
    CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
    CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
    ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
    ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGTTTTA CTAAAGGGAG GAAAAAGAGA GGAAGAGAAA
    CCATTTAGAG ACTGTGCAGA TGTATATCAA GCTGGTTTTA ATAAAAGTGG AATCTACACT
    ATTTATATTA ATAATATGCC AGAACCCAAA AAGGTGTTTT GCAATATGGA TGTCAATGGG
    GGAGGTTGGA CTGTAATACA ACATCGTGAA GATGGAAGTC TAGATTTCCA AAGAGGCTGG
    AAGGAATATA AAATGGGTTT TGGAAATCCC TCCGGTGAAT ATTGGCTGGG GAATGAGTTT
    ATTTTTGCCA TTACCAGTCA GAGGCAGTAC ATGCTAAGAA TTGAGTTAAT GGACTGGGAA
    GGGAACCGAG CCTATTCACA GTATGACAGA TTCCACATAG GAAATGAAAA GCAAAACTAT
    AGGTTGTATT TAAAAGGTCA CACTGGGACA GCAGGAAAAC AGAGCAGCCT GATCTTACAC
    GGTGCTGATT TCAGCACTAA AGATGCTGAT AATGACAACT GTATGTGCAA ATGTGCCCTC
    ATGTTAACAG GAGGATGGTG GTTTGATGCT TGTGGCCCCT CCAATCTAAA TGGAATGTTC
    TATACTGCGG GACAAAACCA TGGAAAACTG AATGGGATAA AGTGGCACTA CTTCAAAGGG
    CCCAGTTACT CCTTACGTTC CACAACTATG ATGATTCGAC CTTTAGATTT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu06136
    Clone ID Related Accession (Same CDS sequence) NM_001199859.1 , NM_001199859.2 , NM_001199859.3
    Accession Version NM_001199859.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product angiopoietin-1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX648814.1, AB084454.1 and AP000428.3. On May 31, 2019 this sequence version replaced NM_001199859.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1, which results in an isoform (2) that is one amino acid shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.228403.1, SRR1803614.256349.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
    AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
    TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
    CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
    CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
    GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
    CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
    CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
    ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
    ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGTTTTA CTAAAGGGAG GAAAAAGAGA GGAAGAGAAA
    CCATTTAGAG ACTGTGCAGA TGTATATCAA GCTGGTTTTA ATAAAAGTGG AATCTACACT
    ATTTATATTA ATAATATGCC AGAACCCAAA AAGGTGTTTT GCAATATGGA TGTCAATGGG
    GGAGGTTGGA CTGTAATACA ACATCGTGAA GATGGAAGTC TAGATTTCCA AAGAGGCTGG
    AAGGAATATA AAATGGGTTT TGGAAATCCC TCCGGTGAAT ATTGGCTGGG GAATGAGTTT
    ATTTTTGCCA TTACCAGTCA GAGGCAGTAC ATGCTAAGAA TTGAGTTAAT GGACTGGGAA
    GGGAACCGAG CCTATTCACA GTATGACAGA TTCCACATAG GAAATGAAAA GCAAAACTAT
    AGGTTGTATT TAAAAGGTCA CACTGGGACA GCAGGAAAAC AGAGCAGCCT GATCTTACAC
    GGTGCTGATT TCAGCACTAA AGATGCTGAT AATGACAACT GTATGTGCAA ATGTGCCCTC
    ATGTTAACAG GAGGATGGTG GTTTGATGCT TGTGGCCCCT CCAATCTAAA TGGAATGTTC
    TATACTGCGG GACAAAACCA TGGAAAACTG AATGGGATAA AGTGGCACTA CTTCAAAGGG
    CCCAGTTACT CCTTACGTTC CACAACTATG ATGATTCGAC CTTTAGATTT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186788.1
    CDS361..1854
    Translation

    Target ORF information:

    RefSeq Version NM_001199859.3
    Organism Homo sapiens(human)
    Definition Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199859.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACAGTTT TCCTTTCCTT TGCTTTCCTC GCTGCCATTC TGACTCACAT AGGGTGCAGC 
    AATCAGCGCC GAAGTCCAGA AAACAGTGGG AGAAGATATA ACCGGATTCA ACATGGGCAA
    TGTGCCTACA CTTTCATTCT TCCAGAACAC GATGGCAACT GTCGTGAGAG TACGACAGAC
    CAGTACAACA CAAACGCTCT GCAGAGAGAT GCTCCACACG TGGAACCGGA TTTCTCTTCC
    CAGAAACTTC AACATCTGGA ACATGTGATG GAAAATTATA CTCAGTGGCT GCAAAAACTT
    GAGAATTACA TTGTGGAAAA CATGAAGTCG GAGATGGCCC AGATACAGCA GAATGCAGTT
    CAGAACCACA CGGCTACCAT GCTGGAGATA GGAACCAGCC TCCTCTCTCA GACTGCAGAG
    CAGACCAGAA AGCTGACAGA TGTTGAGACC CAGGTACTAA ATCAAACTTC TCGACTTGAG
    ATACAGCTGC TGGAGAATTC ATTATCCACC TACAAGCTAG AGAAGCAACT TCTTCAACAG
    ACAAATGAAA TCTTGAAGAT CCATGAAAAA AACAGTTTAT TAGAACATAA AATCTTAGAA
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGTTTTA CTAAAGGGAG GAAAAAGAGA GGAAGAGAAA
    CCATTTAGAG ACTGTGCAGA TGTATATCAA GCTGGTTTTA ATAAAAGTGG AATCTACACT
    ATTTATATTA ATAATATGCC AGAACCCAAA AAGGTGTTTT GCAATATGGA TGTCAATGGG
    GGAGGTTGGA CTGTAATACA ACATCGTGAA GATGGAAGTC TAGATTTCCA AAGAGGCTGG
    AAGGAATATA AAATGGGTTT TGGAAATCCC TCCGGTGAAT ATTGGCTGGG GAATGAGTTT
    ATTTTTGCCA TTACCAGTCA GAGGCAGTAC ATGCTAAGAA TTGAGTTAAT GGACTGGGAA
    GGGAACCGAG CCTATTCACA GTATGACAGA TTCCACATAG GAAATGAAAA GCAAAACTAT
    AGGTTGTATT TAAAAGGTCA CACTGGGACA GCAGGAAAAC AGAGCAGCCT GATCTTACAC
    GGTGCTGATT TCAGCACTAA AGATGCTGAT AATGACAACT GTATGTGCAA ATGTGCCCTC
    ATGTTAACAG GAGGATGGTG GTTTGATGCT TGTGGCCCCT CCAATCTAAA TGGAATGTTC
    TATACTGCGG GACAAAACCA TGGAAAACTG AATGGGATAA AGTGGCACTA CTTCAAAGGG
    CCCAGTTACT CCTTACGTTC CACAACTATG ATGATTCGAC CTTTAGATTT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102700
    Clone ID Related Accession (Same CDS sequence) NM_001314051.1
    Accession Version NM_001314051.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product angiopoietin-1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300319.1, BC152411.1 and AP000428.3. Transcript Variant: This variant (3) represents use of an alternate promoter and therefore differs in the 5' UTR and 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream start codon and result in an isoform (3) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300319.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA 
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGGTGTT TTACTAAAGG GAGGAAAAAG AGAGGAAGAG
    AAACCATTTA GAGACTGTGC AGATGTATAT CAAGCTGGTT TTAATAAAAG TGGAATCTAC
    ACTATTTATA TTAATAATAT GCCAGAACCC AAAAAGGTGT TTTGCAATAT GGATGTCAAT
    GGGGGAGGTT GGACTGTAAT ACAACATCGT GAAGATGGAA GTCTAGATTT CCAAAGAGGC
    TGGAAGGAAT ATAAAATGGG TTTTGGAAAT CCCTCCGGTG AATATTGGCT GGGGAATGAG
    TTTATTTTTG CCATTACCAG TCAGAGGCAG TACATGCTAA GAATTGAGTT AATGGACTGG
    GAAGGGAACC GAGCCTATTC ACAGTATGAC AGATTCCACA TAGGAAATGA AAAGCAAAAC
    TATAGGTTGT ATTTAAAAGG TCACACTGGG ACAGCAGGAA AACAGAGCAG CCTGATCTTA
    CACGGTGCTG ATTTCAGCAC TAAAGATGCT GATAATGACA ACTGTATGTG CAAATGTGCC
    CTCATGTTAA CAGGAGGATG GTGGTTTGAT GCTTGTGGCC CCTCCAATCT AAATGGAATG
    TTCTATACTG CGGGACAAAA CCATGGAAAA CTGAATGGGA TAAAGTGGCA CTACTTCAAA
    GGGCCCAGTT ACTCCTTACG TTCCACAACT ATGATGATTC GACCTTTAGA TTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001300980.1
    CDS280..1176
    Translation

    Target ORF information:

    RefSeq Version NM_001314051.1
    Organism Homo sapiens(human)
    Definition Homo sapiens angiopoietin 1 (ANGPT1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001314051.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAAGGAA AACACAAGGA AGAGTTGGAC ACCTTAAAGG AAGAGAAAGA GAACCTTCAA 
    GGCTTGGTTA CTCGTCAAAC ATATATAATC CAGGAGCTGG AAAAGCAATT AAACAGAGCT
    ACCACCAACA ACAGTGTCCT TCAGAAGCAG CAACTGGAGC TGATGGACAC AGTCCACAAC
    CTTGTCAATC TTTGCACTAA AGAAGGTGTT TTACTAAAGG GAGGAAAAAG AGAGGAAGAG
    AAACCATTTA GAGACTGTGC AGATGTATAT CAAGCTGGTT TTAATAAAAG TGGAATCTAC
    ACTATTTATA TTAATAATAT GCCAGAACCC AAAAAGGTGT TTTGCAATAT GGATGTCAAT
    GGGGGAGGTT GGACTGTAAT ACAACATCGT GAAGATGGAA GTCTAGATTT CCAAAGAGGC
    TGGAAGGAAT ATAAAATGGG TTTTGGAAAT CCCTCCGGTG AATATTGGCT GGGGAATGAG
    TTTATTTTTG CCATTACCAG TCAGAGGCAG TACATGCTAA GAATTGAGTT AATGGACTGG
    GAAGGGAACC GAGCCTATTC ACAGTATGAC AGATTCCACA TAGGAAATGA AAAGCAAAAC
    TATAGGTTGT ATTTAAAAGG TCACACTGGG ACAGCAGGAA AACAGAGCAG CCTGATCTTA
    CACGGTGCTG ATTTCAGCAC TAAAGATGCT GATAATGACA ACTGTATGTG CAAATGTGCC
    CTCATGTTAA CAGGAGGATG GTGGTTTGAT GCTTGTGGCC CCTCCAATCT AAATGGAATG
    TTCTATACTG CGGGACAAAA CCATGGAAAA CTGAATGGGA TAAAGTGGCA CTACTTCAAA
    GGGCCCAGTT ACTCCTTACG TTCCACAACT ATGATGATTC GACCTTTAGA TTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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