GTF3A cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | GTF3A |
| Entrez Gene ID | 2971 |
| Full Name | general transcription factor IIIA |
| Synonyms | AP2,TFIIIA |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. [provided by RefSeq, Jul 2008]. |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_002097.2 | NP_002088.2 | transcription factor IIIA |
| Homo sapiens (human) | GTF3A | NP_002088.2 |
| Mus musculus (house mouse) | Gtf3a | NP_079928.2 |
| Rattus norvegicus (Norway rat) | Gtf3a | NP_001107042.1 |
| Gallus gallus (chicken) | GTF3A | NP_001107043.1 |
| Drosophila melanogaster (fruit fly) | CG9609 | NP_573161.1 |
| Magnaporthe oryzae (rice blast fungus) | MGG_05322 | XP_003712886.1 |
| Pan troglodytes (chimpanzee) | GTF3A | XP_522641.3 |
| GTF3A | XP_001095556.1 | |
| Canis lupus familiaris (dog) | GTF3A | XP_534523.3 |
| Bos taurus (cattle) | GTF3A | NP_001107044.1 |
| Danio rerio (zebrafish) | gtf3aa | NP_001003866.1 |
| Schizosaccharomyces pombe (fission yeast) | sfc2 | NP_594670.1 |
| Xenopus tropicalis (tropical clawed frog) | gtf3a | XP_002941582.2 |
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The 5S RNP couples p53 homeostasis to ribosome biogenesis and nucleolar stress.
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Association analyses of 249,796 individuals reveal 18 new loci associated with body mass index.
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cDNA cloning, DNA binding, and evolution of mammalian transcription factor IIIA.
Hanas JS, Hocker JR, Cheng YG, Lerner MR, Brackett DJ, Lightfoot SA, Hanas RJ, Madhusudhan KT, Moreland RJ
Gene282(1-2)43-52(2002 Jan)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The following GTF3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GTF3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu32111 | |
| Clone ID Related Accession (Same CDS sequence) | NM_002097.2 | |
| Accession Version | NM_002097.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ® Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-02 | |
| Organism | Homo sapiens(human) | |
| Product | transcription factor IIIA | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D32257.1, BP254755.1, U20272.1 and AW627732.1. On or before Mar 29, 2008 this sequence version replaced XM_001716951.1, XM_001715623.1, XM_001717641.1, NM_002097.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D32257.1, SRR1163655.444445.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | CTGGATCCGC CGGCCGTGGT CGCCGAGTCG GTGTCGTCCT TGACCATCGC CGACGCGTTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_002088.2 |
| CDS | 195..1292 |
| Misc Feature(1) | 57..59 |
| Misc Feature(2) | 318..386 |
| Misc Feature(3) | 327..386 |
| Misc Feature(4) | order(339..341,345..347,351..353,357..362,369..374,381..383,429..431,435..437,447..452,459..464,471..473,519..521,525..527,531..533,537..542,549..554) |
| Misc Feature(5) | <384..755 |
| Misc Feature(6) | 408..476 |
| Misc Feature(7) | 408..476 |
| Misc Feature(8) | order(408..410,423..425,462..464,474..476) |
| Misc Feature(9) | 498..569 |
| Misc Feature(10) | 498..554 |
| Misc Feature(11) | 594..662 |
| Misc Feature(12) | 603..662 |
| Misc Feature(13) | order(615..617,621..623,627..629,633..638,645..650,657..659,705..707,711..713,723..728,735..740,747..749,864..866,870..872,876..878,882..887,894..899,906..908) |
| Misc Feature(14) | 684..752 |
| Misc Feature(15) | 684..752 |
| Misc Feature(16) | order(684..686,699..701,738..740,750..752) |
| Misc Feature(17) | 765..833 |
| Misc Feature(18) | 849..911 |
| Misc Feature(19) | 849..911 |
| Misc Feature(20) | order(849..851,858..860,897..899,909..911) |
| Misc Feature(21) | 930..1007 |
| Misc Feature(22) | order(930..932,951..953,990..992,1005..1007) |
| Misc Feature(23) | 936..1007 |
| Misc Feature(24) | 1029..1097 |
| Misc Feature(25) | 1029..1097 |
| Misc Feature(26) | order(1029..1031,1044..1046,1083..1085,1095..1097) |
| Exon (1) | 1..395 |
| Exon (2) | 396..496 |
| Exon (3) | 497..593 |
| Exon (4) | 594..682 |
| Exon (5) | 683..756 |
| Exon (6) | 757..837 |
| Exon (7) | 838..1067 |
| Exon (8) | 1068..1127 |
| Exon (9) | 1128..1404 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_002097.2 |
ORF Insert Sequence:
1 | CTGGATCCGC CGGCCGTGGT CGCCGAGTCG GTGTCGTCCT TGACCATCGC CGACGCGTTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
The 5S RNP couples p53 homeostasis to ribosome biogenesis and nucleolar stress. |
|
Association analyses of 249,796 individuals reveal 18 new loci associated with body mass index. |
|
Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening. |
|
Assembly and isolation of intermediate steps of transcription complexes formed on the human 5S rRNA gene. |
|
cDNA cloning, DNA binding, and evolution of mammalian transcription factor IIIA. |