EEF2K cDNA ORF clone, Homo sapiens (human)
| Gene Symbol | EEF2K |
| Entrez Gene ID | 29904 |
| Full Name | eukaryotic elongation factor 2 kinase |
| Synonyms | HSU93850, eEF-2K |
| Gene Type | protein-coding |
| Organism | Homo sapiens (human) |
| Genome | |
| Summary | This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]. lac of sum |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_013302 | NP_037434 | eukaryotic elongation factor 2 kinase |
| Homo sapiens (human) | EEF2K | NP_037434.1 |
| Pan troglodytes (chimpanzee) | EEF2K | XP_001161085.2 |
| Macaca mulatta (Rhesus monkey) | EEF2K | XP_001092565.1 |
| Canis lupus familiaris (dog) | EEF2K | XP_005621491.1 |
| Bos taurus (cattle) | EEF2K | NP_001179471.1 |
| Mus musculus (house mouse) | Eef2k | NP_001254640.1 |
| Rattus norvegicus (Norway rat) | Eef2k | NP_037079.1 |
| Gallus gallus (chicken) | EEF2K | XP_004945358.1 |
| Danio rerio (zebrafish) | eef2k | NP_001002740.1 |
| Caenorhabditis elegans | efk-1 | NP_001022572.1 |
| Xenopus (Silurana) tropicalis (western clawed frog) | eef2k | NP_001120146.1 |
Related articles in PubMed
- Interlaboratory reproducibility of large-scale human protein-complex analysis by standardized AP-MS. Varjosalo M, et al. Nat Methods, 2013 Apr. PMID 23455922.
- Phosphorylation of eukaryotic elongation factor 2 (eEF2) by cyclin A-cyclin-dependent kinase 2 regulates its inhibition by eEF2 kinase. Hizli AA, et al. Mol Cell Biol, 2013 Feb. PMID 23184662.
- Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass. Povlsen LK, et al. Nat Cell Biol, 2012 Oct. PMID 23000965.
- Roles of eEF-2 kinase in cancer. Liu XY, et al. Chin Med J (Engl), 2012 Aug. PMID 22932089.
- Targeted silencing of elongation factor 2 kinase suppresses growth and sensitizes tumors to doxorubicin in an orthotopic model of breast cancer. Tekedereli I, et al. PLoS One, 2012. PMID 22911754.
See all (44) citations in PubMed
See citations in PubMed for homologs of this gene provided by HomoloGene
GeneRIFs: Gene References Into Functions What's a GeneRIF?
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the disruption of eEF-2K expression in breast cancer cells results in the down-regulation of signaling pathways affecting growth, survival and resistance and has potential as a therapeutic approach for the treatment of breast cancer.
Title: Targeted silencing of elongation factor 2 kinase suppresses growth and sensitizes tumors to doxorubicin in an orthotopic model of breast cancer.
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EEF2 phosphorylation by cyclin A-cyclin-dependent kinase 2 (CDK2) on a novel site, serine 595 (S595), directly regulates T56 phosphorylation by eEF2K.
Title: Phosphorylation of eukaryotic elongation factor 2 (eEF2) by cyclin A-cyclin-dependent kinase 2 regulates its inhibition by eEF2 kinase.
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eEF-2 kinase fills critical niches in the life of a cancer cell and the eEF-2/eEF-2 kinase pathway is a key biochemical sensor.
Title: Roles of eEF-2 kinase in cancer.
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Burn induces prolonged activation of eEF2K and eEF2 in pediatric patients.
Title: Thermal injury activates the eEF2K-dependent eEF2 pathway in pediatric patients.
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These data indicate that eEF-2K is regulated at multiple levels with phosphorylation playing a critical role in the enzyme's turnover under stressful conditions.
Title: Phosphorylation of elongation factor-2 kinase differentially regulates the enzyme's stability under stress conditions.
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several autophosphorylation sites, including Thr(348), Thr(353), Ser(366) and Ser(445), all of which are highly conserved among vertebrate
Title: Identification of autophosphorylation sites in eukaryotic elongation factor-2 kinase.
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Phosphorylation of Ser-500 lags behind the phosphorylation of Thr-348 and is associated with the Ca(2+)-independent activity of eEF-2K(eEF-2K)
Title: Calcium/calmodulin stimulates the autophosphorylation of elongation factor 2 kinase on Thr-348 and Ser-500 to regulate its activity and calcium dependence.
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highly conserved residues in the C-terminal tip of eEF2K are essential for the phosphorylation of eEF2
Title: Insights into the regulation of eukaryotic elongation factor 2 kinase and the interplay between its domains.
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results suggest that the expression of eEF-2 kinase contributes to migration and invasion of human glioma cells by protecting them from anoikis.
Title: Expression of elongation factor-2 kinase contributes to anoikis resistance and invasion of human glioma cells.
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activation of eEF-2 kinase-mediated autophagy plays a protective role for cancer cells under metabolic stress conditions
Title: Cytoprotective effect of the elongation factor-2 kinase-mediated autophagy in breast cancer cells subjected to growth factor inhibition.
The following EEF2K gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF2K cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
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| CloneID | OHu13523  |
|
| Clone ID Related Accession (Same CDS sequence) | NM_013302 , XM_005276497 | |
| Accession Version | NM_013302.3 | Documents for ORF clone product in dufault vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | COA |
| ORF Insert Method | CloneEZ® Seamless cloning technology | |
| Structure | linear | |
| Update Date | 15-MAR-2015 | |
| Organism | Homo sapiens (human) | |
| Product | eukaryotic elongation factor 2 kinase | |
| Comment | REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA735877.1, DA556486.1, U93850.1, CA397138.1, AW675420.1 and AC009034.10. On Mar 16, 2006 this sequence version replaced gi:25453477. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_037434.1 |
| CDS | 486..2663 |
| Misc Feature(1) | 465..467(+) |
| Misc Feature(2) | 489..491(+) |
| Misc Feature(3) | 537..539(+) |
| Misc Feature(4) | 537..539(+) |
| Misc Feature(5) | 537..539(+) |
| Misc Feature(6) | 564..566(+) |
| Misc Feature(7) | 576..578(+) |
| Misc Feature(8) | 666..668(+) |
| Misc Feature(9) | 681..683(+) |
| Misc Feature(10) | 690..692(+) |
| Misc Feature(11) | 693..695(+) |
| Misc Feature(12) | 696..698(+) |
| Misc Feature(13) | 717..719(+) |
| Misc Feature(14) | 717..719(+) |
| Misc Feature(15) | 717..719(+) |
| Misc Feature(16) | 846..1439(+) |
| Misc Feature(17) | 1527..1529(+) |
| Misc Feature(18) | 1542..1544(+) |
| Misc Feature(19) | 1560..1562(+) |
| Misc Feature(20) | 1560..1562(+) |
| Misc Feature(21) | 1581..1583(+) |
| Misc Feature(22) | 1581..1583(+) |
| Misc Feature(23) | 1581..1583(+) |
| Misc Feature(24) | 1581..1583(+) |
| Misc Feature(25) | 1581..1583(+) |
| Misc Feature(26) | 1581..1583(+) |
| Misc Feature(27) | 1614..1616(+) |
| Misc Feature(28) | 1614..1616(+) |
| Misc Feature(29) | 1614..1616(+) |
| Misc Feature(30) | 1671..1673(+) |
| Misc Feature(31) | 1671..1673(+) |
| Misc Feature(32) | 1671..1673(+) |
| Misc Feature(33) | 1671..1673(+) |
| Misc Feature(34) | 1671..1673(+) |
| Misc Feature(35) | 1671..1673(+) |
| Misc Feature(36) | 1677..1679(+) |
| Misc Feature(37) | 1677..1679(+) |
| Misc Feature(38) | 1677..1679(+) |
| Misc Feature(39) | 1818..1820(+) |
| Misc Feature(40) | 1893..1895(+) |
| Misc Feature(41) | 1893..1895(+) |
| Misc Feature(42) | 1905..1907(+) |
| Misc Feature(43) | 1905..1907(+) |
| Misc Feature(44) | 1914..1916(+) |
| Misc Feature(45) | 1956..1958(+) |
| Misc Feature(46) | 1983..1985(+) |
| Misc Feature(47) | 1983..1985(+) |
| Misc Feature(48) | 2082..>2624(+) |
| Misc Feature(49) | 2124..2213(+) |
| Misc Feature(50) | 2262..2351(+) |
| Misc Feature(51) | 2262..2351(+) |
| Misc Feature(52) | 2265..2315(+) |
| Misc Feature(53) | 2313..2366(+) |
| Misc Feature(54) | 2382..2516(+) |
| Misc Feature(55) | 2538..2627(+) |
| Exon (1) | 1..409 Gene:EEF2K Gene Synonym: |
| Exon (2) | 410..731 Gene:EEF2K Gene Synonym: |
| Exon (3) | 732..832 Gene:EEF2K Gene Synonym: |
| Exon (4) | 833..893 Gene:EEF2K Gene Synonym: |
| Exon (5) | 894..931 Gene:EEF2K Gene Synonym: |
| Exon (6) | 932..1103 Gene:EEF2K Gene Synonym: |
| Exon (7) | 1104..1253 Gene:EEF2K Gene Synonym: |
| Exon (8) | 1254..1386 Gene:EEF2K Gene Synonym: |
| Exon (9) | 1387..1514 Gene:EEF2K Gene Synonym: |
| Exon (10) | 1515..1716 Gene:EEF2K Gene Synonym: |
| Exon (11) | 1717..1784 Gene:EEF2K Gene Synonym: |
| Exon (12) | 1785..1862 Gene:EEF2K Gene Synonym: |
| Exon (13) | 1863..1925 Gene:EEF2K Gene Synonym: |
| Exon (14) | 1926..2060 Gene:EEF2K Gene Synonym: |
| Exon (15) | 2061..2249 Gene:EEF2K Gene Synonym: |
| Exon (16) | 2250..2374 Gene:EEF2K Gene Synonym: |
| Exon (17) | 2375..2553 Gene:EEF2K Gene Synonym: |
| Exon (18) | 2554..7412 Gene:EEF2K Gene Synonym: |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_013302 |
ORF Insert Sequence:
1 | ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
A conserved loop in the catalytic domain of eukaryotic elongation factor 2 kinase plays a key role in its substrate specificity |
|
Targeting elongation factor-2 kinase (eEF-2K) induces apoptosis in human pancreatic cancer cells |
|
Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation |
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The eEF2 kinase confers resistance to nutrient deprivation by blocking translation elongation |
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Phosphorylation of eukaryotic elongation factor 2 (eEF2) by cyclin A-cyclin-dependent kinase 2 regulates its inhibition by eEF2 kinase |
|
Phosphorylation of elongation factor-2 kinase on serine 499 by cAMP-dependent protein kinase induces Ca2+/calmodulin-independent activity |
|
Mapping the functional domains of elongation factor-2 kinase |
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Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase |
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Regulation of elongation factor-2 by multisite phosphorylation |
|
Identification of the major Mr 100,000 substrate for calmodulin-dependent protein kinase III in mammalian cells as elongation factor-2 |
