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EEF2K cDNA ORF clone, Homo sapiens(Human)

Gene Symbol EEF2K
Entrez Gene ID 29904
Full Name eukaryotic elongation factor 2 kinase
Synonyms HSU93850,eEF-2K
General protein information
Preferred Names

eukaryotic elongation factor 2 kinase

Names

eukaryotic elongation factor 2 kinase
calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase
calmodulin-dependent protein kinase III
eEF-2 kinase
elongation factor-2 kinase
eukaroytic elongation factor 2 kinase

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

16

16p12.2

Summary This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008].
Disorder MIM:

606968


mRNA and Protein(s)

mRNA Protein Name
NM_013302.3 NP_037434.1 eukaryotic elongation factor 2 kinase
XM_017023193.1 XP_016878682.1 eukaryotic elongation factor 2 kinase isoform X1
XM_017023194.1 XP_016878683.1 eukaryotic elongation factor 2 kinase isoform X2



Homo sapiens (human) EEF2K NP_037434.1
Bos taurus (cattle) EEF2K NP_001179471.1
Canis lupus familiaris (dog) EEF2K XP_005621491.1
Macaca mulatta (rhesus monkey) EEF2K XP_001092565.1
Pan troglodytes (chimpanzee) EEF2K XP_001161085.2
Rattus norvegicus (Norway rat) Eef2k NP_037079.1
Danio rerio (zebrafish) eef2k NP_001002740.1
Caenorhabditis elegans (roundworm) efk-1 NP_001022572.1
Xenopus tropicalis (tropical clawed frog) eef2k NP_001120146.1
Mus musculus (house mouse) Eef2k NP_001254640.1
Gallus gallus (chicken) EEF2K XP_004945358.1


Related articles in PubMed

Eukaryotic elongation factor 2 kinase as a drug target in cancer, and in cardiovascular and neurodegenerative diseases.
Liu R, Proud CG
Acta pharmacologica Sinica37(3)285-94(2016 Mar)

Regulation and roles of elongation factor 2 kinase.
Proud CG
Biochemical Society transactions43(3)328-32(2015 Jun)

Molecular Mechanism for the Control of Eukaryotic Elongation Factor 2 Kinase by pH: Role in Cancer Cell Survival.
Xie J, Mikolajek H, Pigott CR, Hooper KJ, Mellows T, Moore CE, Mohammed H, Werner JM, Thomas GJ, Proud CG
Molecular and cellular biology35(10)1805-24(2015 May)

Elongation Factor 2 Kinase Is Regulated by Proline Hydroxylation and Protects Cells during Hypoxia.
Moore CE, Mikolajek H, Regufe da Mota S, Wang X, Kenney JW, Werner JM, Proud CG
Molecular and cellular biology35(10)1788-804(2015 May)

Regulated stability of eukaryotic elongation factor 2 kinase requires intrinsic but not ongoing activity.
Wang X, Xie J, da Mota SR, Moore CE, Proud CG
The Biochemical journal467(2)321-31(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study reports how phosphorylation of a regulatory site (Ser-500) integrates with Ca(2+) and CaM to influence eEF-2K activity.
Title: Signal Integration at Elongation Factor 2 Kinase: THE ROLES OF CALCIUM, CALMODULIN, AND SER-500 PHOSPHORYLATION.

Article reviews recent evidence concerning the role of eEF2K in human diseases; growing evidence links eEF2K to a range of human diseases, including cardiovascular conditions (atherosclerosis, via macrophage survival) and pulmonary arterial hypertension, as well as solid tumors, where eEF2K appears to play contrasting roles depending on tumor type and stage. eEF2K is also involved in neurological disorders. [Review]
Title: Eukaryotic elongation factor 2 kinase as a drug target in cancer, and in cardiovascular and neurodegenerative diseases.

Recent evidence shows that eEF2K plays an important role in learning and memory, processes that require the synthesis of new proteins and involve Ca-mediated signalling. eEF2K is activated under conditions of nutrient and energy depletion
Title: Regulation and roles of elongation factor 2 kinase.

Results show that eEF2K is rapidly activated in response to acidosis in cells, an effect that is followed by its downregulation.
Title: Molecular Mechanism for the Control of Eukaryotic Elongation Factor 2 Kinase by pH: Role in Cancer Cell Survival.

Data show that eEF2K is activated during hypoxia or upon inhibition of prolyl hydroxylases and inhibited by its hydroxylation on a highly conserved proline residue, restricting its activity during normoxia.
Title: Elongation Factor 2 Kinase Is Regulated by Proline Hydroxylation and Protects Cells during Hypoxia.

The following EEF2K gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF2K cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu13523 NM_013302.3
Latest version!
Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu86007 XM_017023193.1
Latest version!
Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00
OHu86008 XM_017023194.1
Latest version!
Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu13523
Clone ID Related Accession (Same CDS sequence) NM_013302.3
Accession Version NM_013302.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-16
Organism Homo sapiens(Human)
Product eukaryotic elongation factor 2 kinase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA735877.1, DA556486.1, U93850.1, CA397138.1, AW675420.1 and AC009034.10. On Mar 16, 2006 this sequence version replaced NM_013302.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC
CGAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_037434.1
CDS486..2663
Misc Feature(1)465..467
Misc Feature(2)489..491
Misc Feature(3)537..539
Misc Feature(4)537..539
Misc Feature(5)537..539
Misc Feature(6)564..566
Misc Feature(7)564..566
Misc Feature(8)576..578
Misc Feature(9)666..668
Misc Feature(10)681..683
Misc Feature(11)690..692
Misc Feature(12)693..695
Misc Feature(13)693..695
Misc Feature(14)696..698
Misc Feature(15)699..701
Misc Feature(16)705..707
Misc Feature(17)717..719
Misc Feature(18)717..719
Misc Feature(19)717..719
Misc Feature(20)726..767
Misc Feature(21)846..1439
Misc Feature(22)1212..1214
Misc Feature(23)1527..1529
Misc Feature(24)1542..1544
Misc Feature(25)1560..1562
Misc Feature(26)1560..1562
Misc Feature(27)1581..1583
Misc Feature(28)1581..1583
Misc Feature(29)1581..1583
Misc Feature(30)1581..1583
Misc Feature(31)1581..1583
Misc Feature(32)1581..1583
Misc Feature(33)1614..1616
Misc Feature(34)1614..1616
Misc Feature(35)1614..1616
Misc Feature(36)1659..1661
Misc Feature(37)1671..1673
Misc Feature(38)1671..1673
Misc Feature(39)1671..1673
Misc Feature(40)1671..1673
Misc Feature(41)1671..1673
Misc Feature(42)1671..1673
Misc Feature(43)1677..1679
Misc Feature(44)1677..1679
Misc Feature(45)1677..1679
Misc Feature(46)1788..1790
Misc Feature(47)1818..1820
Misc Feature(48)1893..1895
Misc Feature(49)1893..1895
Misc Feature(50)1905..1907
Misc Feature(51)1905..1907
Misc Feature(52)1914..1916
Misc Feature(53)1956..1958
Misc Feature(54)1983..1985
Misc Feature(55)1983..1985
Misc Feature(56)2082..>2624
Misc Feature(57)2124..2213
Misc Feature(58)2262..2351
Exon (1)1..409
Exon (2)410..731
Exon (3)732..832
Exon (4)833..893
Exon (5)894..931
Exon (6)932..1103
Exon (7)1104..1253
Exon (8)1254..1386
Exon (9)1387..1514
Exon (10)1515..1716
Exon (11)1717..1784
Exon (12)1785..1862
Exon (13)1863..1925
Exon (14)1926..2060
Exon (15)2061..2249
Exon (16)2250..2374
Exon (17)2375..2553
Exon (18)2554..7412
Translation

Target ORF information:

RefSeq Version NM_013302.3
Organism Homo sapiens(Human)
Definition Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_013302.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC
CGAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86007
Clone ID Related Accession (Same CDS sequence) XM_017023193.1
Accession Version XM_017023193.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product eukaryotic elongation factor 2 kinase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC
CAAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016878682.1
CDS197..2374
Misc Feature(1)557..1150
Misc Feature(2)1793..>2335
Misc Feature(3)1835..1924
Misc Feature(4)1973..2062
Translation

Target ORF information:

RefSeq Version XM_017023193.1
Organism Homo sapiens(Human)
Definition Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017023193.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC
CAAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86008
Clone ID Related Accession (Same CDS sequence) XM_017023194.1
Accession Version XM_017023194.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2115bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product eukaryotic elongation factor 2 kinase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC
CAAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGTA
TGTGTAGAGA AGTGGAATCT CCTCAACTCC TCCCGCCTCC ACCTGCCGAG GGCTTCGGCC
GTGGCCCTGG AAGTGCAAAG GCTTAATGCT CTGGACCTCG AAAAGAAAAT CGGGAAGTCC
ATTTTGGGGA AGGTCCATCT GGCCATGGTG CGCTACCACG AGGGTGGGCG CTTCTGCGAG
AAGGGCGAGG AGTGGGACCA GGAGTCGGCT GTCTTCCACC TGGAGCACGC AGCCAACCTG
GGCGAGCTGG AGGCCATCGT GGGCCTGGGA CTCATGTACT CGCAGTTGCC TCATCACATC
CTAGCCGATG TCTCTCTGAA GGAGACAGAA GAGAACAAAA CCAAAGGATT TGATTACTTA
CTAAAGGCCG CTGAAGCTGG CGACAGGCAG TCCATGATCC TAGTGGCGCG AGCTTTTGAC
TCTGGCCAGA ACCTCAGCCC GGACAGGTGC CAAGACTGGC TAGAGGCCCT GCACTGGTAC
AACACTGCCC TGGAGATGAC GGACTGTGAT GAGGGCGGTG AGTACGACGG AATGCAGGAC
GAGCCCCGGT ACATGATGCT GGCCAGGGAG GCCGAGATGC TGTTCACAGG AGGCTACGGG
CTGGAGAAGG ACCCGCAGAG ATCAGGGGAC TTGTATACCC AGGCAGCAGA GGCAGCGATG
GAAGCCATGA AGGGCCGACT GGCCAACCAG TACTACCAAA AGGCTGAAGA GGCCTGGGCC
CAGATGGAGG AGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016878683.1
CDS493..2607
Translation

Target ORF information:

RefSeq Version XM_017023194.1
Organism Homo sapiens(Human)
Definition Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017023194.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC
CAAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGTA
TGTGTAGAGA AGTGGAATCT CCTCAACTCC TCCCGCCTCC ACCTGCCGAG GGCTTCGGCC
GTGGCCCTGG AAGTGCAAAG GCTTAATGCT CTGGACCTCG AAAAGAAAAT CGGGAAGTCC
ATTTTGGGGA AGGTCCATCT GGCCATGGTG CGCTACCACG AGGGTGGGCG CTTCTGCGAG
AAGGGCGAGG AGTGGGACCA GGAGTCGGCT GTCTTCCACC TGGAGCACGC AGCCAACCTG
GGCGAGCTGG AGGCCATCGT GGGCCTGGGA CTCATGTACT CGCAGTTGCC TCATCACATC
CTAGCCGATG TCTCTCTGAA GGAGACAGAA GAGAACAAAA CCAAAGGATT TGATTACTTA
CTAAAGGCCG CTGAAGCTGG CGACAGGCAG TCCATGATCC TAGTGGCGCG AGCTTTTGAC
TCTGGCCAGA ACCTCAGCCC GGACAGGTGC CAAGACTGGC TAGAGGCCCT GCACTGGTAC
AACACTGCCC TGGAGATGAC GGACTGTGAT GAGGGCGGTG AGTACGACGG AATGCAGGAC
GAGCCCCGGT ACATGATGCT GGCCAGGGAG GCCGAGATGC TGTTCACAGG AGGCTACGGG
CTGGAGAAGG ACCCGCAGAG ATCAGGGGAC TTGTATACCC AGGCAGCAGA GGCAGCGATG
GAAGCCATGA AGGGCCGACT GGCCAACCAG TACTACCAAA AGGCTGAAGA GGCCTGGGCC
CAGATGGAGG AGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Eukaryotic elongation factor 2 kinase as a drug target in cancer, and in cardiovascular and neurodegenerative diseases.
Acta pharmacologica Sinica37(3)285-94(2016 Mar)
Liu R,Proud CG


book

Regulation and roles of elongation factor 2 kinase.
Biochemical Society transactions43(3)328-32(2015 Jun)
Proud CG


book

Molecular Mechanism for the Control of Eukaryotic Elongation Factor 2 Kinase by pH: Role in Cancer Cell Survival.
Molecular and cellular biology35(10)1805-24(2015 May)
Xie J,Mikolajek H,Pigott CR,Hooper KJ,Mellows T,Moore CE,Mohammed H,Werner JM,Thomas GJ,Proud CG


book

Elongation Factor 2 Kinase Is Regulated by Proline Hydroxylation and Protects Cells during Hypoxia.
Molecular and cellular biology35(10)1788-804(2015 May)
Moore CE,Mikolajek H,Regufe da Mota S,Wang X,Kenney JW,Werner JM,Proud CG


book

Regulated stability of eukaryotic elongation factor 2 kinase requires intrinsic but not ongoing activity.
The Biochemical journal467(2)321-31(2015 Apr)
Wang X,Xie J,da Mota SR,Moore CE,Proud CG


 
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