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EEF2K cDNA ORF clone, Homo sapiens (human)

Gene Symbol EEF2K
Entrez Gene ID 29904
Full Name eukaryotic elongation factor 2 kinase
Synonyms HSU93850, eEF-2K
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16p12.2

Summary This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

606968


mRNA and Protein(s)

mRNA Protein Name
NM_013302 NP_037434 eukaryotic elongation factor 2 kinase



Homo sapiens (human) EEF2K NP_037434.1
Pan troglodytes (chimpanzee) EEF2K XP_001161085.2
Macaca mulatta (Rhesus monkey) EEF2K XP_001092565.1
Canis lupus familiaris (dog) EEF2K XP_005621491.1
Bos taurus (cattle) EEF2K NP_001179471.1
Mus musculus (house mouse) Eef2k NP_001254640.1
Rattus norvegicus (Norway rat) Eef2k NP_037079.1
Gallus gallus (chicken) EEF2K XP_004945358.1
Danio rerio (zebrafish) eef2k NP_001002740.1
Caenorhabditis elegans efk-1 NP_001022572.1
Xenopus (Silurana) tropicalis (western clawed frog) eef2k NP_001120146.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EEF2K gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF2K cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu13523
NM_013302 Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu13523
Clone ID Related Accession (Same CDS sequence) NM_013302 , XM_005276497
Accession Version NM_013302.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2178bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product eukaryotic elongation factor 2 kinase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA735877.1, DA556486.1, U93850.1, CA397138.1, AW675420.1 and AC009034.10. On Mar 16, 2006 this sequence version replaced gi:25453477. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC
CGAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_037434.1
CDS486..2663
Misc Feature(1)465..467(+)
Misc Feature(2)489..491(+)
Misc Feature(3)537..539(+)
Misc Feature(4)537..539(+)
Misc Feature(5)537..539(+)
Misc Feature(6)564..566(+)
Misc Feature(7)576..578(+)
Misc Feature(8)666..668(+)
Misc Feature(9)681..683(+)
Misc Feature(10)690..692(+)
Misc Feature(11)693..695(+)
Misc Feature(12)696..698(+)
Misc Feature(13)717..719(+)
Misc Feature(14)717..719(+)
Misc Feature(15)717..719(+)
Misc Feature(16)846..1439(+)
Misc Feature(17)1527..1529(+)
Misc Feature(18)1542..1544(+)
Misc Feature(19)1560..1562(+)
Misc Feature(20)1560..1562(+)
Misc Feature(21)1581..1583(+)
Misc Feature(22)1581..1583(+)
Misc Feature(23)1581..1583(+)
Misc Feature(24)1581..1583(+)
Misc Feature(25)1581..1583(+)
Misc Feature(26)1581..1583(+)
Misc Feature(27)1614..1616(+)
Misc Feature(28)1614..1616(+)
Misc Feature(29)1614..1616(+)
Misc Feature(30)1671..1673(+)
Misc Feature(31)1671..1673(+)
Misc Feature(32)1671..1673(+)
Misc Feature(33)1671..1673(+)
Misc Feature(34)1671..1673(+)
Misc Feature(35)1671..1673(+)
Misc Feature(36)1677..1679(+)
Misc Feature(37)1677..1679(+)
Misc Feature(38)1677..1679(+)
Misc Feature(39)1818..1820(+)
Misc Feature(40)1893..1895(+)
Misc Feature(41)1893..1895(+)
Misc Feature(42)1905..1907(+)
Misc Feature(43)1905..1907(+)
Misc Feature(44)1914..1916(+)
Misc Feature(45)1956..1958(+)
Misc Feature(46)1983..1985(+)
Misc Feature(47)1983..1985(+)
Misc Feature(48)2082..>2624(+)
Misc Feature(49)2124..2213(+)
Misc Feature(50)2262..2351(+)
Misc Feature(51)2262..2351(+)
Misc Feature(52)2265..2315(+)
Misc Feature(53)2313..2366(+)
Misc Feature(54)2382..2516(+)
Misc Feature(55)2538..2627(+)
Exon (1)1..409
Gene:EEF2K
Gene Synonym:
Exon (2)410..731
Gene:EEF2K
Gene Synonym:
Exon (3)732..832
Gene:EEF2K
Gene Synonym:
Exon (4)833..893
Gene:EEF2K
Gene Synonym:
Exon (5)894..931
Gene:EEF2K
Gene Synonym:
Exon (6)932..1103
Gene:EEF2K
Gene Synonym:
Exon (7)1104..1253
Gene:EEF2K
Gene Synonym:
Exon (8)1254..1386
Gene:EEF2K
Gene Synonym:
Exon (9)1387..1514
Gene:EEF2K
Gene Synonym:
Exon (10)1515..1716
Gene:EEF2K
Gene Synonym:
Exon (11)1717..1784
Gene:EEF2K
Gene Synonym:
Exon (12)1785..1862
Gene:EEF2K
Gene Synonym:
Exon (13)1863..1925
Gene:EEF2K
Gene Synonym:
Exon (14)1926..2060
Gene:EEF2K
Gene Synonym:
Exon (15)2061..2249
Gene:EEF2K
Gene Synonym:
Exon (16)2250..2374
Gene:EEF2K
Gene Synonym:
Exon (17)2375..2553
Gene:EEF2K
Gene Synonym:
Exon (18)2554..7412
Gene:EEF2K
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_013302
Organism Homo sapiens (human)
Definition Homo sapiens eukaryotic elongation factor 2 kinase (EEF2K), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_013302

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA 
GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC
CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC
AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC
TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT
GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC
ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA
GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG
TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG
TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG
CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA
CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC
TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC
TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT
CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA
GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC
ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC
AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC
CGAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT
GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC
ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC
ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC
AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC
CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG
GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG
GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG
TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGC
GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC
CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC
ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC
TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT
GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG
TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG
GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC
GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG
ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG
GCCCAGATGG AGGAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A conserved loop in the catalytic domain of eukaryotic elongation factor 2 kinase plays a key role in its substrate specificity
Mol. Cell. Biol. 34 (12), 2294-2307 (2014)
Moore CE, Regufe da Mota S, Mikolajek H and Proud CG.


book

Targeting elongation factor-2 kinase (eEF-2K) induces apoptosis in human pancreatic cancer cells
Apoptosis 19 (1), 241-258 (2014)
Ashour AA, Abdel-Aziz AA, Mansour AM, Alpay SN, Huo L and Ozpolat B.


book

Eukaryotic elongation factor 2 kinase regulates the development of hypertension through oxidative stress-dependent vascular inflammation
Am. J. Physiol. Heart Circ. Physiol. 305 (5), H756-H768 (2013)
Usui T, Okada M, Hara Y and Yamawaki H.


book

The eEF2 kinase confers resistance to nutrient deprivation by blocking translation elongation
Cell 153 (5), 1064-1079 (2013)
Leprivier G, Remke M, Rotblat B, Dubuc A, Mateo AR, Kool M, Agnihotri S, El-Naggar A, Yu B, Somasekharan SP, Faubert B, Bridon G, Tognon CE, Mathers J, Thomas R, Li A, Barokas A, Kwok B, Bowden M, Smith S, Wu X, Korshunov A, Hielscher T, Northcott PA, Galpin JD, Ahern CA, Wang Y, McCabe MG, Collins VP, Jones RG, Pollak M, Delattre O, Gleave ME, Jan E, Pfister SM, Proud CG, Derry WB, Taylor MD and Sorensen PH.


book

Phosphorylation of eukaryotic elongation factor 2 (eEF2) by cyclin A-cyclin-dependent kinase 2 regulates its inhibition by eEF2 kinase
Mol. Cell. Biol. 33 (3), 596-604 (2013)
Hizli AA, Chi Y, Swanger J, Carter JH, Liao Y, Welcker M, Ryazanov AG and Clurman BE.


book

Phosphorylation of elongation factor-2 kinase on serine 499 by cAMP-dependent protein kinase induces Ca2+/calmodulin-independent activity
Biochem. J. 353 (PT 3), 621-626 (2001)
Diggle TA, Subkhankulova T, Lilley KS, Shikotra N, Willis AE and Redpath NT.


book

Mapping the functional domains of elongation factor-2 kinase
Biochemistry 39 (40), 12216-12224 (2000)
Pavur KS, Petrov AN and Ryazanov AG.


book

Identification of a new class of protein kinases represented by eukaryotic elongation factor-2 kinase
Proc. Natl. Acad. Sci. U.S.A. 94 (10), 4884-4889 (1997)
Ryazanov AG, Ward MD, Mendola CE, Pavur KS, Dorovkov MV, Wiedmann M, Erdjument-Bromage H, Tempst P, Parmer TG, Prostko CR, Germino FJ and Hait WN.


book

Regulation of elongation factor-2 by multisite phosphorylation
Eur. J. Biochem. 213 (2), 689-699 (1993)
Redpath NT, Price NT, Severinov KV and Proud CG.


book

Identification of the major Mr 100,000 substrate for calmodulin-dependent protein kinase III in mammalian cells as elongation factor-2
J. Biol. Chem. 262 (36), 17299-17303 (1987)
Nairn AC and Palfrey HC.


 
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