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HBA1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol HBA1
Entrez Gene ID 3039
Full Name hemoglobin, alpha 1
Synonyms HBA-T3, HBH
General protein information
Preferred Names
hemoglobin subunit alpha
Names
hemoglobin subunit alpha
alpha-globin
alpha-1 globin
alpha-1-globin
alpha one globin
hemoglobin alpha chain
hemoglobin alpha-1 chain
hemoglobin alpha 1 globin chain
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

16

16p13.3

Summary The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

141800

Disorder Html: Thalassemias, alpha-, 604131 (3); Methemoglobinemias, alpha- (3);

mRNA and Protein(s)

mRNA Protein Name
NM_000558 NP_000549 hemoglobin subunit alpha



Homo sapiens (human) HBA2 NP_000508.1
Homo sapiens (human) HBA1 NP_000549.1
Macaca mulatta (Rhesus monkey) HBA2 NP_001038189.1
Macaca mulatta (Rhesus monkey) HBA2 XP_001094404.1
Canis lupus familiaris (dog) LOC100855558 NP_001257814.1
Canis lupus familiaris (dog) LOC100855540 NP_001257815.1
Bos taurus (cattle) HBA NP_001070890.2
Bos taurus (cattle) HBA1 XP_003585623.1
Mus musculus (house mouse) Hba-a2 NP_001077424.1
Mus musculus (house mouse) Hba-a1 NP_032244.2
Rattus norvegicus (Norway rat) LOC287167 NP_001013875.1
Gallus gallus (chicken) HBAA NP_001004376.1
Gallus gallus (chicken) LOC100858011 XP_004949966.1
Xenopus (Silurana) tropicalis (western clawed frog) hba1 NP_988860.1

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HBA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HBA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu23534
NM_000558 Homo sapiens hemoglobin, alpha 1 (HBA1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23534
Clone ID Related Accession (Same CDS sequence) NM_000517 , NM_000558
Accession Version NM_000558.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 429bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 01-APR-2015
Organism Homo sapiens (human)
Product hemoglobin subunit alpha
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z84721.1, BC101846.1 and AI064720.1. This sequence is a reference standard in the RefSeqGene project. On Aug 9, 2014 this sequence version replaced gi:14456711. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK223392.1, CB994937.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
ATGGTGCTGT CTCCTGCCGA CAAGACCAAC GTCAAGGCCG CCTGGGGTAA GGTCGGCGCG 
CACGCTGGCG AGTATGGTGC GGAGGCCCTG GAGAGGATGT TCCTGTCCTT CCCCACCACC
AAGACCTACT TCCCGCACTT CGACCTGAGC CACGGCTCTG CCCAGGTTAA GGGCCACGGC
AAGAAGGTGG CCGACGCGCT GACCAACGCC GTGGCGCACG TGGACGACAT GCCCAACGCG
CTGTCCGCCC TGAGCGACCT GCACGCGCAC AAGCTTCGGG TGGACCCGGT CAACTTCAAG
CTCCTAAGCC ACTGCCTGCT GGTGACCCTG GCCGCCCACC TCCCCGCCGA GTTCACCCCT
GCGGTGCACG CCTCCCTGGA CAAGTTCCTG GCTTCTGTGA GCACCGTGCT GACCTCCAAA
TACCGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000549.1
CDS67..495
Misc Feature(1)30..30(+)
Misc Feature(2)70..72(+)
Misc Feature(3)73..492(+)
Misc Feature(4)76..78(+)
Misc Feature(5)88..90(+)
Misc Feature(6)88..90(+)
Misc Feature(7)91..93(+)
Misc Feature(8)100..102(+)
Misc Feature(9)100..102(+)
Misc Feature(10)115..117(+)
Misc Feature(11)115..117(+)
Misc Feature(12)115..117(+)
Misc Feature(13)139..141(+)
Misc Feature(14)139..141(+)
Misc Feature(15)160..492(+)
Misc Feature(16)163..477(+)
Misc Feature(17)172..174(+)
Misc Feature(18)187..189(+)
Misc Feature(19)193..195(+)
Misc Feature(20)214..216(+)
Misc Feature(21)235..237(+)
Misc Feature(22)247..249(+)
Misc Feature(23)250..252(+)
Misc Feature(24)337..339(+)
Misc Feature(25)364..366(+)
Exon (1)1..161
Gene:HBA1
Gene Synonym:
Exon (2)162..366
Gene:HBA1
Gene Synonym:
Exon (3)367..606
Gene:HBA1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000558
Organism Homo sapiens (human)
Definition Homo sapiens hemoglobin, alpha 1 (HBA1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000558

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGGTGCTGT CTCCTGCCGA CAAGACCAAC GTCAAGGCCG CCTGGGGTAA GGTCGGCGCG 
CACGCTGGCG AGTATGGTGC GGAGGCCCTG GAGAGGATGT TCCTGTCCTT CCCCACCACC
AAGACCTACT TCCCGCACTT CGACCTGAGC CACGGCTCTG CCCAGGTTAA GGGCCACGGC
AAGAAGGTGG CCGACGCGCT GACCAACGCC GTGGCGCACG TGGACGACAT GCCCAACGCG
CTGTCCGCCC TGAGCGACCT GCACGCGCAC AAGCTTCGGG TGGACCCGGT CAACTTCAAG
CTCCTAAGCC ACTGCCTGCT GGTGACCCTG GCCGCCCACC TCCCCGCCGA GTTCACCCCT
GCGGTGCACG CCTCCCTGGA CAAGTTCCTG GCTTCTGTGA GCACCGTGCT GACCTCCAAA
TACCGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Alpha thalassemia allelic frequency in Lebanon
Pediatr Blood Cancer 62 (1), 120-122 (2015)
Farra C, Badra R, Fares F, Muwakkit S, Dbaibo G, Dabbous I, Ashkar H, Mounsef C and Abboud MR.


book

Experimental basis for a new allosteric model for multisubunit proteins
Proc. Natl. Acad. Sci. U.S.A. 111 (35), 12758-12763 (2014)
Viappiani C, Abbruzzetti S, Ronda L, Bettati S, Henry ER, Mozzarelli A and Eaton WA.


book

Glycated hemoglobin predicts overall and cardiovascular mortality in non-diabetic hemodialysis patients
Clin. Nephrol. 82 (3), 173-180 (2014)
Ok ES, Asci G, Toz H, Ritz E, Kircelli F, Sever MS, Ozkahya M, Sipahi S, Dheir H, Bozkurt D, Omer Z, Sahin OZ, Ertilav M and Ok E.


book

The associations of SEA-alpha thalassemia 1, XmnI-Ggamma polymorphism and beta-globin gene mutations with the clinical severity of beta-thalassemia syndrome in northern Thailand
J Med Assoc Thai 97 (3), 300-307 (2014)
Tatu,T., Sritong,W. and Sa-Nguansermsri,T.


book

Identification and characterization of two novel and differentially expressed isoforms of human alpha2- and alpha1-globin genes
Hemoglobin 36 (5), 421-432 (2012)
Ghassemifar R, Forster L, Qadah T and Finlayson J.


book

Alpha-Thalassemia
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Origa,R., Moi,P., Galanello,R. and Cao,A.


book

Hemoglobin Moabit: alpha 86 (F7) Leu leads to Arg: a new unstable abnormal hemoglobin
Acta Haematol. 61 (3), 121-124 (1979)
Knuth,A., Pribilla,W., Marti,H.R. and Winterhalter,K.H.


book

Hemoglobin J Rovigo (alpha53 Ala replaced by Asp) in association with beta-thalassemia
Hemoglobin 2 (5), 443-445 (1978)
Moo-Penn,W.F., Jue,D.L. and Baine,R.M.


book

The nucleotide sequences of the untranslated 5' regions of human alpha- and beta-globin mRNAs
Proc. Natl. Acad. Sci. U.S.A. 74 (11), 5145-5149 (1977)
Chang,J.C., Temple,G.F., Poon,R., Neumann,K.H. and Kan,Y.W.


book

Haemoglobin G Norfolk alpha 85 (F6) Asp leads to Asn. Structural characterization by sequenator analysis and functional properties of a new variant with high oxygen affinity
FEBS Lett. 50 (2), 163-167 (1975)
Cohen-Solal,M., Manesse,B., Thillet,J. and Rosa,J.


 
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