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HGD cDNA ORF clone, Homo sapiens(Human)

Gene Symbol HGD
Entrez Gene ID 3081
Full Name homogentisate 1,2-dioxygenase
Synonyms AKU,HGO
General protein information
Preferred Names

homogentisate 1,2-dioxygenase

Names

homogentisate 1,2-dioxygenase
homogentisate oxidase
homogentisate oxygenase
homogentisic acid oxidase
homogentisicase

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

3

3q13.33

Summary This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.[provided by RefSeq, May 2010].
Disorder MIM:

607474

Disorder Html: Alkaptonuria, 203500 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005247412.1 XP_005247469.1 homogentisate 1,2-dioxygenase isoform X1
XM_005247413.1 XP_005247470.1 homogentisate 1,2-dioxygenase isoform X2
XM_011512746.1 XP_011511048.1 homogentisate 1,2-dioxygenase isoform X4
XM_017006277.1 XP_016861766.1 homogentisate 1,2-dioxygenase isoform X3
XM_005247414.4 XP_005247471.1 homogentisate 1,2-dioxygenase isoform X5
NM_000187.3 NP_000178.2 homogentisate 1,2-dioxygenase



Drosophila melanogaster (fruit fly) hgo NP_523544.2
Oryza sativa (rice) Os06g0103300 NP_001056540.1
Homo sapiens (human) HGD NP_000178.2
Bos taurus (cattle) HGD NP_001138324.1
Danio rerio (zebrafish) hgd NP_694498.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP009609 XP_318638.4
Caenorhabditis elegans (roundworm) hgo-1 NP_492433.1
Canis lupus familiaris (dog) HGD XP_535754.3
Mus musculus (house mouse) Hgd NP_038575.2
Gallus gallus (chicken) HGD XP_416553.1
Magnaporthe oryzae (rice blast fungus) MGG_00431 XP_003718640.1
Xenopus tropicalis (tropical clawed frog) hgd XP_004912503.1
Pan troglodytes (chimpanzee) HGD XP_001164938.1
Rattus norvegicus (Norway rat) Hgd NP_001012145.1
Neurospora crassa NCU05499 XP_960461.1
Arabidopsis thaliana (thale cress) HGO NP_851187.1
Macaca mulatta (rhesus monkey) HGD XP_001110996.1


Related articles in PubMed

First report of HGD mutations in a Chinese with alkaptonuria.
Yang YJ, Guo JH, Chen WJ, Zhao R, Tang JS, Meng XH, Zhao L, Tu M, He XY, Wu LQ, Zhu YM
Gene518(2)467-9(2013 Apr)

Homogentisate 1,2 dioxygenase is expressed in human osteoarticular cells: implications in alkaptonuria.
Laschi M, Tinti L, Braconi D, Millucci L, Ghezzi L, Amato L, Selvi E, Spreafico A, Bernardini G, Santucci A
Journal of cellular physiology227(9)3254-7(2012 Sep)

An update on molecular genetics of Alkaptonuria (AKU).
Zatkova A
Journal of inherited metabolic disease34(6)1127-36(2011 Dec)

The human homogentisate 1,2-dioxygenase (HGO) gene.
Granadino B, Beltrán-Valero de Bernabé D, Fernández-Cañón JM, Peñalva MA, Rodríguez de Córdoba S
Genomics43(2)115-22(1997 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Twelve novel HGD gene variants have been identified in 99 alkaptonuria patients affecting the bones.
Title: Twelve novel HGD gene variants identified in 99 alkaptonuria patients: focus on 'black bone disease' in Italy.

A novel alkaptonuria mutation, c.87 + 1G > A, shows a significant founder effect and high prevalence in a nomadic Indian population.
Title: Mutation screening of the HGD gene identifies a novel alkaptonuria mutation with significant founder effect and high prevalence.

study described two novel HGD mutations in a Chinese alkaptonuria family, the splicing mutation of IVS7 1G>C, a donor splice site of exon 7, and a missense mutation of F329C in exon 12
Title: First report of HGD mutations in a Chinese with alkaptonuria.

The observed increase of HGD expression in Alkaptonuria cells is probably due to a compensatory mechanism to overcome the almost null catalytic activity of the deficient enzyme
Title: Homogentisate 1,2 dioxygenase is expressed in human osteoarticular cells: implications in alkaptonuria.

An update on molecular genetics of Alkaptonuria (AKU).(review)
Title: An update on molecular genetics of Alkaptonuria (AKU).

The following HGD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HGD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu20740 NM_000187.3
Latest version!
Homo sapiens homogentisate 1,2-dioxygenase (HGD), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
OHu37912 XM_005247412.1
Latest version!
Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu37913 XM_005247413.1
Latest version!
Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu37914 XM_005247414.4
Latest version!
Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu58205 XM_011512746.1
Latest version!
Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu94120 XM_017006277.1
Latest version!
Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu20740
Clone ID Related Accession (Same CDS sequence) NM_000187.3
Accession Version NM_000187.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-04-23
Organism Homo sapiens(Human)
Product homogentisate 1,2-dioxygenase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB231845.1, AK313563.1, AC133474.2 and AA861603.1. This sequence is a reference standard in the RefSeqGene project. On May 13, 2010 this sequence version replaced NM_000187.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313563.1, AF045167.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAG ACCCATCCAT TTTCACAGTA
TTGACTGCTA AGTCTGTCCG CCCTGGAGTG GCCATTGCTG ATTTTGTCAT CTTCCCACCT
CGATGGGGGG TTGCTGATAA GACCTTCAGG CCTCCTTATT ACCATAGGAA CTGCATGAGT
GAGTTCATGG GACTCATCCG AGGTCACTAT GAGGCAAAGC AAGGTGGGTT CCTGCCAGGG
GGAGGGAGTC TACACAGCAC AATGACCCCC CATGGACCTG ATGCTGACTG CTTTGAGAAG
GCCAGCAAGG TCAAGCTGGC ACCTGAGAGG ATTGCCGATG GCACCATGGC ATTTATGTTT
GAATCATCTT TAAGTCTGGC GGTCACAAAG TGGGGACTCA AGGCCTCCAG GTGTTTGGAT
GAGAACTACC ACAAGTGCTG GGAGCCACTC AAGAGCCACT TCACTCCCAA CTCCAGGAAC
CCAGCAGAAC CTAATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000178.2
CDS461..1798
Misc Feature(1)446..448
Misc Feature(2)467..1762
Misc Feature(3)752..754
Exon (1)1..475
Exon (2)476..547
Exon (3)548..636
Exon (4)637..742
Exon (5)743..802
Exon (6)803..894
Exon (7)895..929
Exon (8)930..1009
Exon (9)1010..1109
Exon (10)1110..1234
Exon (11)1235..1339
Exon (12)1340..1466
Exon (13)1467..1648
Exon (14)1649..2010
Translation

Target ORF information:

RefSeq Version NM_000187.3
Organism Homo sapiens(Human)
Definition Homo sapiens homogentisate 1,2-dioxygenase (HGD), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000187.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAG ACCCATCCAT TTTCACAGTA
TTGACTGCTA AGTCTGTCCG CCCTGGAGTG GCCATTGCTG ATTTTGTCAT CTTCCCACCT
CGATGGGGGG TTGCTGATAA GACCTTCAGG CCTCCTTATT ACCATAGGAA CTGCATGAGT
GAGTTCATGG GACTCATCCG AGGTCACTAT GAGGCAAAGC AAGGTGGGTT CCTGCCAGGG
GGAGGGAGTC TACACAGCAC AATGACCCCC CATGGACCTG ATGCTGACTG CTTTGAGAAG
GCCAGCAAGG TCAAGCTGGC ACCTGAGAGG ATTGCCGATG GCACCATGGC ATTTATGTTT
GAATCATCTT TAAGTCTGGC GGTCACAAAG TGGGGACTCA AGGCCTCCAG GTGTTTGGAT
GAGAACTACC ACAAGTGCTG GGAGCCACTC AAGAGCCACT TCACTCCCAA CTCCAGGAAC
CCAGCAGAAC CTAATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37912
Clone ID Related Accession (Same CDS sequence) XM_005247412.1
Accession Version XM_005247412.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1113bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product homogentisate 1,2-dioxygenase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGG ATGTCTCCCC GTTCAATGTT GTGGCCTGGC ACGGGAATTA TACACCCTAC
AAGTACAACC TGAAGAATTT CATGGTTATC AACTCAGTGG CCTTTGACCA TGCAGACCCA
TCCATTTTCA CAGTATTGAC TGCTAAGTCT GTCCGCCCTG GAGTGGCCAT TGCTGATTTT
GTCATCTTCC CACCTCGATG GGGGGTTGCT GATAAGACCT TCAGGCCTCC TTATTACCAT
AGGAACTGCA TGAGTGAGTT CATGGGACTC ATCCGAGGTC ACTATGAGGC AAAGCAAGGT
GGGTTCCTGC CAGGGGGAGG GAGTCTACAC AGCACAATGA CCCCCCATGG ACCTGATGCT
GACTGCTTTG AGAAGGCCAG CAAGGTCAAG CTGGCACCTG AGAGGATTGC CGATGGCACC
ATGGCATTTA TGTTTGAATC ATCTTTAAGT CTGGCGGTCA CAAAGTGGGG ACTCAAGGCC
TCCAGGTGTT TGGATGAGAA CTACCACAAG TGCTGGGAGC CACTCAAGAG CCACTTCACT
CCCAACTCCA GGAACCCAGC AGAACCTAAT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005247469.1
CDS461..1573
Misc Feature(1)467..1537
Translation

Target ORF information:

RefSeq Version XM_005247412.1
Organism Homo sapiens(Human)
Definition Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005247412.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGG ATGTCTCCCC GTTCAATGTT GTGGCCTGGC ACGGGAATTA TACACCCTAC
AAGTACAACC TGAAGAATTT CATGGTTATC AACTCAGTGG CCTTTGACCA TGCAGACCCA
TCCATTTTCA CAGTATTGAC TGCTAAGTCT GTCCGCCCTG GAGTGGCCAT TGCTGATTTT
GTCATCTTCC CACCTCGATG GGGGGTTGCT GATAAGACCT TCAGGCCTCC TTATTACCAT
AGGAACTGCA TGAGTGAGTT CATGGGACTC ATCCGAGGTC ACTATGAGGC AAAGCAAGGT
GGGTTCCTGC CAGGGGGAGG GAGTCTACAC AGCACAATGA CCCCCCATGG ACCTGATGCT
GACTGCTTTG AGAAGGCCAG CAAGGTCAAG CTGGCACCTG AGAGGATTGC CGATGGCACC
ATGGCATTTA TGTTTGAATC ATCTTTAAGT CTGGCGGTCA CAAAGTGGGG ACTCAAGGCC
TCCAGGTGTT TGGATGAGAA CTACCACAAG TGCTGGGAGC CACTCAAGAG CCACTTCACT
CCCAACTCCA GGAACCCAGC AGAACCTAAT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37913
Clone ID Related Accession (Same CDS sequence) XM_005247413.1
Accession Version XM_005247413.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1017bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product homogentisate 1,2-dioxygenase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAG ACCCATCCAT TTTCACAGTA
TTGACTGCTA AGTCTGTCCG CCCTGGAGTG GCCATTGCTG ATTTTGTCAT CTTCCCACCT
CGATGGGGGG TTGCTGATAA GACCTTCAGG CCTCCTTATT ACCATAATCT AGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005247470.1
CDS461..1477
Misc Feature(1)467..>1465
Translation

Target ORF information:

RefSeq Version XM_005247413.1
Organism Homo sapiens(Human)
Definition Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005247413.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAG ACCCATCCAT TTTCACAGTA
TTGACTGCTA AGTCTGTCCG CCCTGGAGTG GCCATTGCTG ATTTTGTCAT CTTCCCACCT
CGATGGGGGG TTGCTGATAA GACCTTCAGG CCTCCTTATT ACCATAATCT AGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37914
Clone ID Related Accession (Same CDS sequence) XM_005247414.4
Accession Version XM_005247414.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product homogentisate 1,2-dioxygenase isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005247414.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGA TTCCTTGGGT
CTTCCTAAAA GGTACCAGAC TGGAAATGCA GTGAATCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005247471.1
CDS461..1162
Misc Feature(1)467..>1141
Translation

Target ORF information:

RefSeq Version XM_005247414.4
Organism Homo sapiens(Human)
Definition Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005247414.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGA TTCCTTGGGT
CTTCCTAAAA GGTACCAGAC TGGAAATGCA GTGAATCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58205
Clone ID Related Accession (Same CDS sequence) XM_011512746.1
Accession Version XM_011512746.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 885bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product homogentisate 1,2-dioxygenase isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511048.1
CDS461..1345
Misc Feature(1)467..>1339
Translation

Target ORF information:

RefSeq Version XM_011512746.1
Organism Homo sapiens(Human)
Definition Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011512746.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu94120
Clone ID Related Accession (Same CDS sequence) XM_017006277.1
Accession Version XM_017006277.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 915bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product homogentisate 1,2-dioxygenase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGAACA GATGCTTTTA CAATTCAGAT GGGGACTTCT TGATTGTTCC GCAGAAAGGG 
AACCTTCTCA TTTACACCGA GTTTGGCAAG ATGCTTGTAC AGCCCAATGA GATCTGCGTC
ATTCAGAGAG GAATGCGGTT CAGCATAGAT GTCTTTGAGG AGACCAGGGG CTACATCTTG
GAGGTCTATG GTGTCCACTT TGAGTTACCT GACCTTGGAC CAATTGGGGC CAATGGCTTG
GCCAATCCTC GTGATTTCTT GATACCCATT GCCTGGTATG AGGATCGCCA AGTACCAGGT
GGTTACACGG TCATTAATAA ATACCAGGGC AAGCTGTTTG CTGCCAAACA GGATGTCTCC
CCGTTCAATG TTGTGGCCTG GCACGGGAAT TATACACCCT ACAAGTACAA CCTGAAGAAT
TTCATGGTTA TCAACTCAGT GGCCTTTGAC CATGCAGACC CATCCATTTT CACAGTATTG
ACTGCTAAGT CTGTCCGCCC TGGAGTGGCC ATTGCTGATT TTGTCATCTT CCCACCTCGA
TGGGGGGTTG CTGATAAGAC CTTCAGGCCT CCTTATTACC ATAGGAACTG CATGAGTGAG
TTCATGGGAC TCATCCGAGG TCACTATGAG GCAAAGCAAG GTGGGTTCCT GCCAGGGGGA
GGGAGTCTAC ACAGCACAAT GACCCCCCAT GGACCTGATG CTGACTGCTT TGAGAAGGCC
AGCAAGGTCA AGCTGGCACC TGAGAGGATT GCCGATGGCA CCATGGCATT TATGTTTGAA
TCATCTTTAA GTCTGGCGGT CACAAAGTGG GGACTCAAGG CCTCCAGGTG TTTGGATGAG
AACTACCACA AGTGCTGGGA GCCACTCAAG AGCCACTTCA CTCCCAACTC CAGGAACCCA
GCAGAACCTA ATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016861766.1
CDS497..1411
Misc Feature(1)<497..1375
Translation

Target ORF information:

RefSeq Version XM_017006277.1
Organism Homo sapiens(Human)
Definition Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017006277.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGAACA GATGCTTTTA CAATTCAGAT GGGGACTTCT TGATTGTTCC GCAGAAAGGG 
AACCTTCTCA TTTACACCGA GTTTGGCAAG ATGCTTGTAC AGCCCAATGA GATCTGCGTC
ATTCAGAGAG GAATGCGGTT CAGCATAGAT GTCTTTGAGG AGACCAGGGG CTACATCTTG
GAGGTCTATG GTGTCCACTT TGAGTTACCT GACCTTGGAC CAATTGGGGC CAATGGCTTG
GCCAATCCTC GTGATTTCTT GATACCCATT GCCTGGTATG AGGATCGCCA AGTACCAGGT
GGTTACACGG TCATTAATAA ATACCAGGGC AAGCTGTTTG CTGCCAAACA GGATGTCTCC
CCGTTCAATG TTGTGGCCTG GCACGGGAAT TATACACCCT ACAAGTACAA CCTGAAGAAT
TTCATGGTTA TCAACTCAGT GGCCTTTGAC CATGCAGACC CATCCATTTT CACAGTATTG
ACTGCTAAGT CTGTCCGCCC TGGAGTGGCC ATTGCTGATT TTGTCATCTT CCCACCTCGA
TGGGGGGTTG CTGATAAGAC CTTCAGGCCT CCTTATTACC ATAGGAACTG CATGAGTGAG
TTCATGGGAC TCATCCGAGG TCACTATGAG GCAAAGCAAG GTGGGTTCCT GCCAGGGGGA
GGGAGTCTAC ACAGCACAAT GACCCCCCAT GGACCTGATG CTGACTGCTT TGAGAAGGCC
AGCAAGGTCA AGCTGGCACC TGAGAGGATT GCCGATGGCA CCATGGCATT TATGTTTGAA
TCATCTTTAA GTCTGGCGGT CACAAAGTGG GGACTCAAGG CCTCCAGGTG TTTGGATGAG
AACTACCACA AGTGCTGGGA GCCACTCAAG AGCCACTTCA CTCCCAACTC CAGGAACCCA
GCAGAACCTA ATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

First report of HGD mutations in a Chinese with alkaptonuria.
Gene518(2)467-9(2013 Apr)
Yang YJ,Guo JH,Chen WJ,Zhao R,Tang JS,Meng XH,Zhao L,Tu M,He XY,Wu LQ,Zhu YM


book

Homogentisate 1,2 dioxygenase is expressed in human osteoarticular cells: implications in alkaptonuria.
Journal of cellular physiology227(9)3254-7(2012 Sep)
Laschi M,Tinti L,Braconi D,Millucci L,Ghezzi L,Amato L,Selvi E,Spreafico A,Bernardini G,Santucci A


book

An update on molecular genetics of Alkaptonuria (AKU).
Journal of inherited metabolic disease34(6)1127-36(2011 Dec)
Zatkova A


book

The human homogentisate 1,2-dioxygenase (HGO) gene.
Genomics43(2)115-22(1997 Jul)
Granadino B,Beltrán-Valero de Bernabé D,Fernández-Cañón JM,Peñalva MA,Rodríguez de Córdoba S


 
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