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HGD cDNA ORF clone, Homo sapiens (human)

Gene Symbol HGD
Entrez Gene ID 3081
Full Name homogentisate 1,2-dioxygenase
Synonyms AKU, HGO
General protein information
Preferred Names
homogentisate 1,2-dioxygenase
Names
homogentisate 1,2-dioxygenase
homogentisicase
homogentisate oxidase
homogentisate oxygenase
homogentisic acid oxidase
NP_000178.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3q13.33

Summary This gene encodes the enzyme homogentisate 1,2 dioxygenase. This enzyme is involved in the catabolism of the amino acids tyrosine and phenylalanine. Mutations in this gene are the cause of the autosomal recessive metabolism disorder alkaptonuria.[provided by RefSeq, May 2010]. lac of sum
Disorder MIM:

607474

Disorder Html: Alkaptonuria, 203500 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_005247412 XP_005247469 homogentisate 1,2-dioxygenase isoform X1
XM_005247413 XP_005247470 homogentisate 1,2-dioxygenase isoform X2
XM_011512746 XP_011511048 homogentisate 1,2-dioxygenase isoform X3
XM_005247414 XP_005247471 homogentisate 1,2-dioxygenase isoform X4
NM_000187 NP_000178 homogentisate 1,2-dioxygenase



Homo sapiens (human) HGD NP_000178.2
Pan troglodytes (chimpanzee) HGD XP_001164938.1
Macaca mulatta (Rhesus monkey) HGD XP_001110996.1
Canis lupus familiaris (dog) HGD XP_535754.3
Bos taurus (cattle) HGD NP_001138324.1
Mus musculus (house mouse) Hgd NP_038575.2
Rattus norvegicus (Norway rat) Hgd NP_001012145.1
Gallus gallus (chicken) HGD XP_416553.1
Danio rerio (zebrafish) hgd NP_694498.2
Drosophila melanogaster (fruit fly) hgo NP_523544.2
Caenorhabditis elegans hgo-1 NP_492433.1
Arabidopsis thaliana (thale cress) HGO NP_851187.1
Xenopus (Silurana) tropicalis (western clawed frog) hgd XP_004912503.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HGD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HGD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu37912 XM_005247412 PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu37913 XM_005247413 PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu58205 XM_011512746 PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu37914 XM_005247414 PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu20740
NM_000187 Homo sapiens homogentisate 1,2-dioxygenase (HGD), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$189.50-$265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu37912
Clone ID Related Accession (Same CDS sequence) XM_005247412
Accession Version XM_005247412.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1113bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product homogentisate 1,2-dioxygenase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGG ATGTCTCCCC GTTCAATGTT GTGGCCTGGC ACGGGAATTA TACACCCTAC
AAGTACAACC TGAAGAATTT CATGGTTATC AACTCAGTGG CCTTTGACCA TGCAGACCCA
TCCATTTTCA CAGTATTGAC TGCTAAGTCT GTCCGCCCTG GAGTGGCCAT TGCTGATTTT
GTCATCTTCC CACCTCGATG GGGGGTTGCT GATAAGACCT TCAGGCCTCC TTATTACCAT
AGGAACTGCA TGAGTGAGTT CATGGGACTC ATCCGAGGTC ACTATGAGGC AAAGCAAGGT
GGGTTCCTGC CAGGGGGAGG GAGTCTACAC AGCACAATGA CCCCCCATGG ACCTGATGCT
GACTGCTTTG AGAAGGCCAG CAAGGTCAAG CTGGCACCTG AGAGGATTGC CGATGGCACC
ATGGCATTTA TGTTTGAATC ATCTTTAAGT CTGGCGGTCA CAAAGTGGGG ACTCAAGGCC
TCCAGGTGTT TGGATGAGAA CTACCACAAG TGCTGGGAGC CACTCAAGAG CCACTTCACT
CCCAACTCCA GGAACCCAGC AGAACCTAAT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005247469.1
CDS461..1573
Misc Feature(1)467..1537(+)
Translation

Target ORF information:

RefSeq Version XM_005247412
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005247412

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGG ATGTCTCCCC GTTCAATGTT GTGGCCTGGC ACGGGAATTA TACACCCTAC
AAGTACAACC TGAAGAATTT CATGGTTATC AACTCAGTGG CCTTTGACCA TGCAGACCCA
TCCATTTTCA CAGTATTGAC TGCTAAGTCT GTCCGCCCTG GAGTGGCCAT TGCTGATTTT
GTCATCTTCC CACCTCGATG GGGGGTTGCT GATAAGACCT TCAGGCCTCC TTATTACCAT
AGGAACTGCA TGAGTGAGTT CATGGGACTC ATCCGAGGTC ACTATGAGGC AAAGCAAGGT
GGGTTCCTGC CAGGGGGAGG GAGTCTACAC AGCACAATGA CCCCCCATGG ACCTGATGCT
GACTGCTTTG AGAAGGCCAG CAAGGTCAAG CTGGCACCTG AGAGGATTGC CGATGGCACC
ATGGCATTTA TGTTTGAATC ATCTTTAAGT CTGGCGGTCA CAAAGTGGGG ACTCAAGGCC
TCCAGGTGTT TGGATGAGAA CTACCACAAG TGCTGGGAGC CACTCAAGAG CCACTTCACT
CCCAACTCCA GGAACCCAGC AGAACCTAAT TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37913
Clone ID Related Accession (Same CDS sequence) XM_005247413
Accession Version XM_005247413.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1017bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product homogentisate 1,2-dioxygenase isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAG ACCCATCCAT TTTCACAGTA
TTGACTGCTA AGTCTGTCCG CCCTGGAGTG GCCATTGCTG ATTTTGTCAT CTTCCCACCT
CGATGGGGGG TTGCTGATAA GACCTTCAGG CCTCCTTATT ACCATAATCT AGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005247470.1
CDS461..1477
Misc Feature(1)467..>1465(+)
Translation

Target ORF information:

RefSeq Version XM_005247413
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005247413

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAG ACCCATCCAT TTTCACAGTA
TTGACTGCTA AGTCTGTCCG CCCTGGAGTG GCCATTGCTG ATTTTGTCAT CTTCCCACCT
CGATGGGGGG TTGCTGATAA GACCTTCAGG CCTCCTTATT ACCATAATCT AGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58205
Clone ID Related Accession (Same CDS sequence) XM_011512746
Accession Version XM_011512746.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 885bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product homogentisate 1,2-dioxygenase isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511048.1
CDS461..1345
Misc Feature(1)467..>1339(+)
Translation

Target ORF information:

RefSeq Version XM_011512746
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011512746

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAA TCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37914
Clone ID Related Accession (Same CDS sequence) XM_005247414
Accession Version XM_005247414.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 702bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product homogentisate 1,2-dioxygenase isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578807337. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGA TTCCTTGGGT
CTTCCTAAAA GGTACCAGAC TGGAAATGCA GTGAATCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005247471.1
CDS461..1162
Misc Feature(1)467..>1141(+)
Translation

Target ORF information:

RefSeq Version XM_005247414
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens homogentisate 1,2-dioxygenase (HGD), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005247414

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGA TTCCTTGGGT
CTTCCTAAAA GGTACCAGAC TGGAAATGCA GTGAATCTTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20740
Clone ID Related Accession (Same CDS sequence) NM_000187
Accession Version NM_000187.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1338bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product homogentisate 1,2-dioxygenase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB231845.1, AK313563.1, AC133474.2 and AA861603.1. This sequence is a reference standard in the RefSeqGene project. On May 13, 2010 this sequence version replaced gi:115527116. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313563.1, AF045167.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAG ACCCATCCAT TTTCACAGTA
TTGACTGCTA AGTCTGTCCG CCCTGGAGTG GCCATTGCTG ATTTTGTCAT CTTCCCACCT
CGATGGGGGG TTGCTGATAA GACCTTCAGG CCTCCTTATT ACCATAGGAA CTGCATGAGT
GAGTTCATGG GACTCATCCG AGGTCACTAT GAGGCAAAGC AAGGTGGGTT CCTGCCAGGG
GGAGGGAGTC TACACAGCAC AATGACCCCC CATGGACCTG ATGCTGACTG CTTTGAGAAG
GCCAGCAAGG TCAAGCTGGC ACCTGAGAGG ATTGCCGATG GCACCATGGC ATTTATGTTT
GAATCATCTT TAAGTCTGGC GGTCACAAAG TGGGGACTCA AGGCCTCCAG GTGTTTGGAT
GAGAACTACC ACAAGTGCTG GGAGCCACTC AAGAGCCACT TCACTCCCAA CTCCAGGAAC
CCAGCAGAAC CTAATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000178.2
CDS461..1798
Misc Feature(1)446..448(+)
Misc Feature(2)467..1762(+)
Misc Feature(3)752..754(+)
Exon (1)1..475
Gene:HGD
Gene Synonym:
Exon (2)476..547
Gene:HGD
Gene Synonym:
Exon (3)548..636
Gene:HGD
Gene Synonym:
Exon (4)637..742
Gene:HGD
Gene Synonym:
Exon (5)743..802
Gene:HGD
Gene Synonym:
Exon (6)803..894
Gene:HGD
Gene Synonym:
Exon (7)895..929
Gene:HGD
Gene Synonym:
Exon (8)930..1009
Gene:HGD
Gene Synonym:
Exon (9)1010..1109
Gene:HGD
Gene Synonym:
Exon (10)1110..1234
Gene:HGD
Gene Synonym:
Exon (11)1235..1339
Gene:HGD
Gene Synonym:
Exon (12)1340..1466
Gene:HGD
Gene Synonym:
Exon (13)1467..1648
Gene:HGD
Gene Synonym:
Exon (14)1649..2010
Gene:HGD
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000187
Organism Homo sapiens (human)
Definition Homo sapiens homogentisate 1,2-dioxygenase (HGD), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000187

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCTGAGT TAAAGTACAT TTCTGGATTT GGGAATGAGT GTTCTTCAGA GGATCCTCGC 
TGCCCAGGTT CCCTGCCAGA AGGACAGAAT AATCCTCAGG TCTGCCCCTA CAATCTCTAT
GCTGAGCAGC TCTCAGGATC GGCTTTCACT TGTCCACGGA GCACCAATAA GAGAAGCTGG
CTGTATAGGA TTCTACCTTC AGTTTCTCAC AAGCCCTTTG AATCCATTGA CGAAGGCCAA
GTCACTCACA ACTGGGATGA AGTTGATCCT GATCCTAACC AGCTTAGATG GAAACCATTT
GAGATTCCAA AAGCATCTCA GAAGAAAGTA GACTTTGTGA GTGGCCTGCA TACCTTGTGT
GGAGCTGGAG ACATAAAGTC TAACAATGGG CTTGCTATCC ACATTTTCCT CTGCAATACC
TCCATGGAGA ACAGATGCTT TTACAATTCA GATGGGGACT TCTTGATTGT TCCGCAGAAA
GGGAACCTTC TCATTTACAC CGAGTTTGGC AAGATGCTTG TACAGCCCAA TGAGATCTGC
GTCATTCAGA GAGGAATGCG GTTCAGCATA GATGTCTTTG AGGAGACCAG GGGCTACATC
TTGGAGGTCT ATGGTGTCCA CTTTGAGTTA CCTGACCTTG GACCAATTGG GGCCAATGGC
TTGGCCAATC CTCGTGATTT CTTGATACCC ATTGCCTGGT ATGAGGATCG CCAAGTACCA
GGTGGTTACA CGGTCATTAA TAAATACCAG GGCAAGCTGT TTGCTGCCAA ACAGGATGTC
TCCCCGTTCA ATGTTGTGGC CTGGCACGGG AATTATACAC CCTACAAGTA CAACCTGAAG
AATTTCATGG TTATCAACTC AGTGGCCTTT GACCATGCAG ACCCATCCAT TTTCACAGTA
TTGACTGCTA AGTCTGTCCG CCCTGGAGTG GCCATTGCTG ATTTTGTCAT CTTCCCACCT
CGATGGGGGG TTGCTGATAA GACCTTCAGG CCTCCTTATT ACCATAGGAA CTGCATGAGT
GAGTTCATGG GACTCATCCG AGGTCACTAT GAGGCAAAGC AAGGTGGGTT CCTGCCAGGG
GGAGGGAGTC TACACAGCAC AATGACCCCC CATGGACCTG ATGCTGACTG CTTTGAGAAG
GCCAGCAAGG TCAAGCTGGC ACCTGAGAGG ATTGCCGATG GCACCATGGC ATTTATGTTT
GAATCATCTT TAAGTCTGGC GGTCACAAAG TGGGGACTCA AGGCCTCCAG GTGTTTGGAT
GAGAACTACC ACAAGTGCTG GGAGCCACTC AAGAGCCACT TCACTCCCAA CTCCAGGAAC
CCAGCAGAAC CTAATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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