HIF1A cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following HIF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26981 NM_001243084.1
Latest version!
Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$339.50-$475.30
$679.00
OHu27176 NM_001530.4
Latest version!
Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu26941 NM_181054.3
Latest version!
Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
View Old Accession Versions >>
OHu27176 NM_001530.3 Homo sapiens hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu26941 NM_181054.2 Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu26981
    Clone ID Related Accession (Same CDS sequence) NM_001243084.1
    Accession Version NM_001243084.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2553bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product hypoxia-inducible factor 1-alpha isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FJ790247.1, CN264320.1, X72726.1 and BC012527.2. Transcript Variant: This variant (3) contains an alternate 5' terminal exon, and uses an alternate in-frame acceptor splice site at the next exon compared to variant 1. This results in a longer isoform (3, also known as isoform I.3) with a distinct N-terminus compared to isoform 1. This isoform is transcriptionally active, and is upregulated in activated human T lymphocytes (PMID:18638657). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ790247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820697 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGCTCCC AATGTCGGAG TTTGGAAAAC AAATTTGTCT TTTTAAAAGA AGGTCTAGGA 
    AACTCAAAAC CTGAAGAATT GGAAGAAATC AGAATAGAAA ATGGTAGGAT AAGTTCTGAA
    CGTCGAAAAG AAAAGTCTCG AGATGCAGCC AGATCTCGGC GAAGTAAAGA ATCTGAAGTT
    TTTTATGAGC TTGCTCATCA GTTGCCACTT CCACATAATG TGAGTTCGCA TCTTGATAAG
    GCCTCTGTGA TGAGGCTTAC CATCAGCTAT TTGCGTGTGA GGAAACTTCT GGATGCTGGT
    GATTTGGATA TTGAAGATGA CATGAAAGCA CAGATGAATT GCTTTTATTT GAAAGCCTTG
    GATGGTTTTG TTATGGTTCT CACAGATGAT GGTGACATGA TTTACATTTC TGATAATGTG
    AACAAATACA TGGGATTAAC TCAGTTTGAA CTAACTGGAC ACAGTGTGTT TGATTTTACT
    CATCCATGTG ACCATGAGGA AATGAGAGAA ATGCTTACAC ACAGAAATGG CCTTGTGAAA
    AAGGGTAAAG AACAAAACAC ACAGCGAAGC TTTTTTCTCA GAATGAAGTG TACCCTAACT
    AGCCGAGGAA GAACTATGAA CATAAAGTCT GCAACATGGA AGGTATTGCA CTGCACAGGC
    CACATTCACG TATATGATAC CAACAGTAAC CAACCTCAGT GTGGGTATAA GAAACCACCT
    ATGACCTGCT TGGTGCTGAT TTGTGAACCC ATTCCTCACC CATCAAATAT TGAAATTCCT
    TTAGATAGCA AGACTTTCCT CAGTCGACAC AGCCTGGATA TGAAATTTTC TTATTGTGAT
    GAAAGAATTA CCGAATTGAT GGGATATGAG CCAGAAGAAC TTTTAGGCCG CTCAATTTAT
    GAATATTATC ATGCTTTGGA CTCTGATCAT CTGACCAAAA CTCATCATGA TATGTTTACT
    AAAGGACAAG TCACCACAGG ACAGTACAGG ATGCTTGCCA AAAGAGGTGG ATATGTCTGG
    GTTGAAACTC AAGCAACTGT CATATATAAC ACCAAGAATT CTCAACCACA GTGCATTGTA
    TGTGTGAATT ACGTTGTGAG TGGTATTATT CAGCACGACT TGATTTTCTC CCTTCAACAA
    ACAGAATGTG TCCTTAAACC GGTTGAATCT TCAGATATGA AAATGACTCA GCTATTCACC
    AAAGTTGAAT CAGAAGATAC AAGTAGCCTC TTTGACAAAC TTAAGAAGGA ACCTGATGCT
    TTAACTTTGC TGGCCCCAGC CGCTGGAGAC ACAATCATAT CTTTAGATTT TGGCAGCAAC
    GACACAGAAA CTGATGACCA GCAACTTGAG GAAGTACCAT TATATAATGA TGTAATGCTC
    CCCTCACCCA ACGAAAAATT ACAGAATATA AATTTGGCAA TGTCTCCATT ACCCACCGCT
    GAAACGCCAA AGCCACTTCG AAGTAGTGCT GACCCTGCAC TCAATCAAGA AGTTGCATTA
    AAATTAGAAC CAAATCCAGA GTCACTGGAA CTTTCTTTTA CCATGCCCCA GATTCAGGAT
    CAGACACCTA GTCCTTCCGA TGGAAGCACT AGACAAAGTT CACCTGAGCC TAATAGTCCC
    AGTGAATATT GTTTTTATGT GGATAGTGAT ATGGTCAATG AATTCAAGTT GGAATTGGTA
    GAAAAACTTT TTGCTGAAGA CACAGAAGCA AAGAACCCAT TTTCTACTCA GGACACAGAT
    TTAGACTTGG AGATGTTAGC TCCCTATATC CCAATGGATG ATGACTTCCA GTTACGTTCC
    TTCGATCAGT TGTCACCATT AGAAAGCAGT TCCGCAAGCC CTGAAAGCGC AAGTCCTCAA
    AGCACAGTTA CAGTATTCCA GCAGACTCAA ATACAAGAAC CTACTGCTAA TGCCACCACT
    ACCACTGCCA CCACTGATGA ATTAAAAACA GTGACAAAAG ACCGTATGGA AGACATTAAA
    ATATTGATTG CATCTCCATC TCCTACCCAC ATACATAAAG AAACTACTAG TGCCACATCA
    TCACCATATA GAGATACTCA AAGTCGGACA GCCTCACCAA ACAGAGCAGG AAAAGGAGTC
    ATAGAACAGA CAGAAAAATC TCATCCAAGA AGCCCTAACG TGTTATCTGT CGCTTTGAGT
    CAAAGAACTA CAGTTCCTGA GGAAGAACTA AATCCAAAGA TACTAGCTTT GCAGAATGCT
    CAGAGAAAGC GAAAAATGGA ACATGATGGT TCACTTTTTC AAGCAGTAGG AATTGGAACA
    TTATTACAGC AGCCAGACGA TCATGCAGCT ACTACATCAC TTTCTTGGAA ACGTGTAAAA
    GGATGCAAAT CTAGTGAACA GAATGGAATG GAGCAAAAGA CAATTATTTT AATACCCTCT
    GATTTAGCAT GTAGACTGCT GGGGCAATCA ATGGATGAAA GTGGATTACC ACAGCTGACC
    AGTTATGATT GTGAAGTTAA TGCTCCTATA CAAGGCAGCA GAAACCTACT GCAGGGTGAA
    GAATTACTCA GAGCTTTGGA TCAAGTTAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001230013.1
    CDS230..2782
    Translation

    Target ORF information:

    RefSeq Version NM_001243084.1
    Organism Homo sapiens(human)
    Definition Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001243084.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGCTCCC AATGTCGGAG TTTGGAAAAC AAATTTGTCT TTTTAAAAGA AGGTCTAGGA 
    AACTCAAAAC CTGAAGAATT GGAAGAAATC AGAATAGAAA ATGGTAGGAT AAGTTCTGAA
    CGTCGAAAAG AAAAGTCTCG AGATGCAGCC AGATCTCGGC GAAGTAAAGA ATCTGAAGTT
    TTTTATGAGC TTGCTCATCA GTTGCCACTT CCACATAATG TGAGTTCGCA TCTTGATAAG
    GCCTCTGTGA TGAGGCTTAC CATCAGCTAT TTGCGTGTGA GGAAACTTCT GGATGCTGGT
    GATTTGGATA TTGAAGATGA CATGAAAGCA CAGATGAATT GCTTTTATTT GAAAGCCTTG
    GATGGTTTTG TTATGGTTCT CACAGATGAT GGTGACATGA TTTACATTTC TGATAATGTG
    AACAAATACA TGGGATTAAC TCAGTTTGAA CTAACTGGAC ACAGTGTGTT TGATTTTACT
    CATCCATGTG ACCATGAGGA AATGAGAGAA ATGCTTACAC ACAGAAATGG CCTTGTGAAA
    AAGGGTAAAG AACAAAACAC ACAGCGAAGC TTTTTTCTCA GAATGAAGTG TACCCTAACT
    AGCCGAGGAA GAACTATGAA CATAAAGTCT GCAACATGGA AGGTATTGCA CTGCACAGGC
    CACATTCACG TATATGATAC CAACAGTAAC CAACCTCAGT GTGGGTATAA GAAACCACCT
    ATGACCTGCT TGGTGCTGAT TTGTGAACCC ATTCCTCACC CATCAAATAT TGAAATTCCT
    TTAGATAGCA AGACTTTCCT CAGTCGACAC AGCCTGGATA TGAAATTTTC TTATTGTGAT
    GAAAGAATTA CCGAATTGAT GGGATATGAG CCAGAAGAAC TTTTAGGCCG CTCAATTTAT
    GAATATTATC ATGCTTTGGA CTCTGATCAT CTGACCAAAA CTCATCATGA TATGTTTACT
    AAAGGACAAG TCACCACAGG ACAGTACAGG ATGCTTGCCA AAAGAGGTGG ATATGTCTGG
    GTTGAAACTC AAGCAACTGT CATATATAAC ACCAAGAATT CTCAACCACA GTGCATTGTA
    TGTGTGAATT ACGTTGTGAG TGGTATTATT CAGCACGACT TGATTTTCTC CCTTCAACAA
    ACAGAATGTG TCCTTAAACC GGTTGAATCT TCAGATATGA AAATGACTCA GCTATTCACC
    AAAGTTGAAT CAGAAGATAC AAGTAGCCTC TTTGACAAAC TTAAGAAGGA ACCTGATGCT
    TTAACTTTGC TGGCCCCAGC CGCTGGAGAC ACAATCATAT CTTTAGATTT TGGCAGCAAC
    GACACAGAAA CTGATGACCA GCAACTTGAG GAAGTACCAT TATATAATGA TGTAATGCTC
    CCCTCACCCA ACGAAAAATT ACAGAATATA AATTTGGCAA TGTCTCCATT ACCCACCGCT
    GAAACGCCAA AGCCACTTCG AAGTAGTGCT GACCCTGCAC TCAATCAAGA AGTTGCATTA
    AAATTAGAAC CAAATCCAGA GTCACTGGAA CTTTCTTTTA CCATGCCCCA GATTCAGGAT
    CAGACACCTA GTCCTTCCGA TGGAAGCACT AGACAAAGTT CACCTGAGCC TAATAGTCCC
    AGTGAATATT GTTTTTATGT GGATAGTGAT ATGGTCAATG AATTCAAGTT GGAATTGGTA
    GAAAAACTTT TTGCTGAAGA CACAGAAGCA AAGAACCCAT TTTCTACTCA GGACACAGAT
    TTAGACTTGG AGATGTTAGC TCCCTATATC CCAATGGATG ATGACTTCCA GTTACGTTCC
    TTCGATCAGT TGTCACCATT AGAAAGCAGT TCCGCAAGCC CTGAAAGCGC AAGTCCTCAA
    AGCACAGTTA CAGTATTCCA GCAGACTCAA ATACAAGAAC CTACTGCTAA TGCCACCACT
    ACCACTGCCA CCACTGATGA ATTAAAAACA GTGACAAAAG ACCGTATGGA AGACATTAAA
    ATATTGATTG CATCTCCATC TCCTACCCAC ATACATAAAG AAACTACTAG TGCCACATCA
    TCACCATATA GAGATACTCA AAGTCGGACA GCCTCACCAA ACAGAGCAGG AAAAGGAGTC
    ATAGAACAGA CAGAAAAATC TCATCCAAGA AGCCCTAACG TGTTATCTGT CGCTTTGAGT
    CAAAGAACTA CAGTTCCTGA GGAAGAACTA AATCCAAAGA TACTAGCTTT GCAGAATGCT
    CAGAGAAAGC GAAAAATGGA ACATGATGGT TCACTTTTTC AAGCAGTAGG AATTGGAACA
    TTATTACAGC AGCCAGACGA TCATGCAGCT ACTACATCAC TTTCTTGGAA ACGTGTAAAA
    GGATGCAAAT CTAGTGAACA GAATGGAATG GAGCAAAAGA CAATTATTTT AATACCCTCT
    GATTTAGCAT GTAGACTGCT GGGGCAATCA ATGGATGAAA GTGGATTACC ACAGCTGACC
    AGTTATGATT GTGAAGTTAA TGCTCCTATA CAAGGCAGCA GAAACCTACT GCAGGGTGAA
    GAATTACTCA GAGCTTTGGA TCAAGTTAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27176
    Clone ID Related Accession (Same CDS sequence) NM_001530.3 , NM_001530.4
    Accession Version NM_001530.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2481bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-17
    Organism Homo sapiens(human)
    Product hypoxia-inducible factor 1-alpha isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC344032.1, BC012527.2 and CN264295.1. This sequence is a reference standard in the RefSeqGene project. On Jul 26, 2008 this sequence version replaced NM_001530.2. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012527.2, SRR1660803.74010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
    AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT
    GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG
    AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT
    GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT
    ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG
    GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC
    CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA
    CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA
    ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA
    TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG
    GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG
    ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC
    GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT
    GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC
    ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA
    GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC
    GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC
    CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA
    GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG
    GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT
    GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC
    GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG
    CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA
    AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT
    CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT
    TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT
    GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG
    ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG
    TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA
    GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC
    ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA
    TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA
    GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA
    GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA
    GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA
    AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAG
    CCAGACGATC ATGCAGCTAC TACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT
    AGTGAACAGA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT
    AGACTGCTGG GGCAATCAAT GGATGAAAGT GGATTACCAC AGCTGACCAG TTATGATTGT
    GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA
    GCTTTGGATC AAGTTAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001521.1
    CDS405..2885
    Translation

    Target ORF information:

    RefSeq Version NM_001530.3
    Organism Homo sapiens(human)
    Definition Homo sapiens hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001530.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
    AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT
    GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG
    AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT
    GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT
    ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG
    GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC
    CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA
    CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA
    ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA
    TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG
    GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG
    ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC
    GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT
    GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC
    ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA
    GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC
    GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC
    CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA
    GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG
    GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT
    GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC
    GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG
    CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA
    AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT
    CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT
    TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT
    GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG
    ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG
    TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA
    GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC
    ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA
    TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA
    GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA
    GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA
    GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA
    AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAG
    CCAGACGATC ATGCAGCTAC TACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT
    AGTGAACAGA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT
    AGACTGCTGG GGCAATCAAT GGATGAAAGT GGATTACCAC AGCTGACCAG TTATGATTGT
    GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA
    GCTTTGGATC AAGTTAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27176
    Clone ID Related Accession (Same CDS sequence) NM_001530.3 , NM_001530.4
    Accession Version NM_001530.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2481bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product hypoxia-inducible factor 1-alpha isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC344032.1, BC012527.2 and CN264295.1. This sequence is a reference standard in the RefSeqGene project. On Dec 7, 2018 this sequence version replaced NM_001530.3. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.144807.1, SRR1660805.134018.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000337138.9/ ENSP00000338018.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
    AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT
    GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG
    AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT
    GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT
    ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG
    GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC
    CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA
    CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA
    ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA
    TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG
    GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG
    ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC
    GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT
    GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC
    ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA
    GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC
    GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC
    CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA
    GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG
    GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT
    GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC
    GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG
    CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA
    AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT
    CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT
    TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT
    GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG
    ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG
    TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA
    GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC
    ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA
    TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA
    GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA
    GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA
    GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA
    AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAG
    CCAGACGATC ATGCAGCTAC TACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT
    AGTGAACAGA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT
    AGACTGCTGG GGCAATCAAT GGATGAAAGT GGATTACCAC AGCTGACCAG TTATGATTGT
    GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA
    GCTTTGGATC AAGTTAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001521.1
    CDS293..2773
    Translation

    Target ORF information:

    RefSeq Version NM_001530.4
    Organism Homo sapiens(human)
    Definition Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001530.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
    AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT
    GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG
    AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT
    GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT
    ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG
    GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC
    CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA
    CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA
    ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA
    TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG
    GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG
    ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC
    GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT
    GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC
    ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA
    GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC
    GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC
    CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA
    GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG
    GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT
    GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC
    GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG
    CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA
    AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT
    CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT
    TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT
    GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG
    ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG
    TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA
    GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC
    ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA
    TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA
    GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA
    GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA
    GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA
    AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAG
    CCAGACGATC ATGCAGCTAC TACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT
    AGTGAACAGA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT
    AGACTGCTGG GGCAATCAAT GGATGAAAGT GGATTACCAC AGCTGACCAG TTATGATTGT
    GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA
    GCTTTGGATC AAGTTAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26941
    Clone ID Related Accession (Same CDS sequence) NM_181054.3 , NM_181054.2
    Accession Version NM_181054.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product hypoxia-inducible factor 1-alpha isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC344032.1, AB073325.1, CN264295.1 and BC012527.2. On Jun 4, 2019 this sequence version replaced NM_181054.2. Transcript Variant: This variant (2) lacks the penultimate coding exon compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.250908.1, SRR1660807.224891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
    AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT
    GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG
    AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT
    GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT
    ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG
    GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC
    CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA
    CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA
    ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA
    TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG
    GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG
    ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC
    GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT
    GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC
    ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA
    GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC
    GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC
    CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA
    GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG
    GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT
    GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC
    GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG
    CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA
    AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT
    CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT
    TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT
    GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG
    ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG
    TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA
    GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC
    ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA
    TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA
    GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA
    GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA
    GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA
    AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_851397.1
    CDS293..2500
    Translation

    Target ORF information:

    RefSeq Version NM_181054.3
    Organism Homo sapiens(human)
    Definition Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_181054.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
    AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT
    GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG
    AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT
    GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT
    ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG
    GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC
    CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA
    CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA
    ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA
    TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG
    GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG
    ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC
    GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT
    GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC
    ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA
    GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC
    GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC
    CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA
    GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG
    GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT
    GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC
    GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG
    CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA
    AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT
    CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT
    TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT
    GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG
    ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG
    TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA
    GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC
    ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA
    TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA
    GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA
    GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA
    GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA
    AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26941
    Clone ID Related Accession (Same CDS sequence) NM_181054.3 , NM_181054.2
    Accession Version NM_181054.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product hypoxia-inducible factor 1-alpha isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC344032.1, AB073325.1, CN264295.1 and BC012527.2. On Jul 26, 2008 this sequence version replaced NM_181054.1. Transcript Variant: This variant (2) lacks the penultimate coding exon compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.263667.1, SRR1660807.17025.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
    AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT
    GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG
    AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT
    GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT
    ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG
    GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC
    CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA
    CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA
    ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA
    TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG
    GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG
    ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC
    GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT
    GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC
    ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA
    GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC
    GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC
    CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA
    GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG
    GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT
    GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC
    GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG
    CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA
    AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT
    CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT
    TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT
    GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG
    ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG
    TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA
    GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC
    ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA
    TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA
    GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA
    GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA
    GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA
    AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_851397.1
    CDS405..2612
    Translation

    Target ORF information:

    RefSeq Version NM_181054.2
    Organism Homo sapiens(human)
    Definition Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_181054.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
    AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT
    GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG
    AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT
    GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT
    ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG
    GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC
    CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA
    CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA
    ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA
    TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG
    GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG
    ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC
    GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT
    GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC
    ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA
    GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC
    GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC
    CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA
    GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG
    GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT
    GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC
    GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG
    CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA
    AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT
    CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT
    TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT
    GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG
    ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG
    TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA
    GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC
    ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA
    TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA
    GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA
    GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA
    GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA
    AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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