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HPCAL1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol HPCAL1
Entrez Gene ID 3241
Full Name hippocalcin like 1
Synonyms BDR1,HLP2,VILIP-3
General protein information
Preferred Names

hippocalcin like 1

Names

hippocalcin-like protein 1
calcium-binding protein BDR-1
visinin-like protein 3
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

2

2p25.1
Summary The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. Several alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Apr 2012].
Disorder MIM:

600207


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_011510349.1 XP_011508651.1 hippocalcin-like protein 1 isoform X2
NM_002149.3 NP_002140.2 hippocalcin-like protein 1
XM_017003952.1 XP_016859441.1 hippocalcin-like protein 1 isoform X1
NM_001258357.1 NP_001245286.1 hippocalcin-like protein 1
NM_134421.2 NP_602293.1 hippocalcin-like protein 1
XM_011510346.1 XP_011508648.1 hippocalcin-like protein 1 isoform X1
XM_011510348.1 XP_011508650.1 hippocalcin-like protein 1 isoform X1
NM_001258358.1 NP_001245287.1 hippocalcin-like protein 1
XM_011510346.2 XP_011508648.1 hippocalcin-like protein 1 isoform X1
XM_017003950.1 XP_016859439.1 hippocalcin-like protein 1 isoform X1
XM_017003951.1 XP_016859440.1 hippocalcin-like protein 1 isoform X1
XM_005246161.1 XP_005246218.1 hippocalcin-like protein 1 isoform X1
XM_005246162.1 XP_005246219.1 hippocalcin-like protein 1 isoform X1
XM_017003953.1 XP_016859442.1 hippocalcin-like protein 1 isoform X1
XM_005246160.1 XP_005246217.1 hippocalcin-like protein 1 isoform X1
XM_011510347.1 XP_011508649.1 hippocalcin-like protein 1 isoform X1
XM_011510345.1 XP_011508647.1 hippocalcin-like protein 1 isoform X1
XM_005246163.1 XP_005246220.1 hippocalcin-like protein 1 isoform X1
NM_001258359.1 NP_001245288.1 hippocalcin-like protein 1

Pathways from BioSystems


Homologies


Rattus norvegicus (Norway rat) Hpcal1 NP_059052.1
Pan troglodytes (chimpanzee) HPCAL1 NP_001233306.1
Gallus gallus (chicken) HPCAL1 NP_990565.1
Xenopus tropicalis (tropical clawed frog) hpcal1 NP_001037893.1
Homo sapiens (human) HPCAL1 NP_602293.1
HPCAL1 XP_001085327.1
Canis lupus familiaris (dog) HPCAL1 XP_005630174.1
Mus musculus (house mouse) Hpcal1 NP_057886.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP007247 XP_001687984.1
Bos taurus (cattle) HPCAL1 NP_001092434.1
Danio rerio (zebrafish) zgc:63695 NP_957458.1
Drosophila melanogaster (fruit fly) Nca NP_788543.1

Gene Ontology


Bibliography

Related articles in PubMed

Hippocalcin-like 1 suppresses hepatocellular carcinoma progression by promoting p21(Waf/Cip1) stabilization by activating the ERK1/2-MAPK pathway.
Zhang Y, Liu Y, Duan J, Yan H, Zhang J, Zhang H, Fan Q, Luo F, Yan G, Qiao K, Liu J
Hepatology (Baltimore, Md.)63(3)880-97(2016 Mar)

Structure and Calcium Binding Properties of a Neuronal Calcium-Myristoyl Switch Protein, Visinin-Like Protein 3.
Li C, Lim S, Braunewell KH, Ames JB
PloS one11(11)e0165921(2016)

The EHEC-host interactome reveals novel targets for the translocated intimin receptor.
Blasche S, Arens S, Ceol A, Siszler G, Schmidt MA, H?user R, Schwarz F, Wuchty S, Aloy P, Uetz P, Stradal T, Koegl M
Scientific reports47531(2014 Dec)

Specific interaction to PIP2 increases the kinetic rate of membrane binding of VILIPs, a subfamily of Neuronal Calcium Sensors (NCS) proteins.
Rebaud S, Wang CK, Sarkis J, Mason L, Simon A, Blum LJ, Hofmann A, Girard-Egrot AP
Biochimica et biophysica acta1838(10)2698-707(2014 Oct)

Mutations that disrupt PHOXB interaction with the neuronal calcium sensor HPCAL1 impede cellular differentiation in neuroblastoma.
Wang W, Zhong Q, Teng L, Bhatnagar N, Sharma B, Zhang X, Luther W 2nd, Haynes LP, Burgoyne RD, Vidal M, Volchenboum S, Hill DE, George RE
Oncogene33(25)3316-24(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Secreted HPCAL1 protein in the plasma dropped dramatically in hepatocellular carcinoma patients relative to controls.
Title: Hippocalcin-like 1 suppresses hepatocellular carcinoma progression by promoting p21(Waf/Cip1) stabilization by activating the ERK1/2-MAPK pathway.

The myristoylated VILIP-3 protein structure determined in this study is quite different.
Title: Structure and Calcium Binding Properties of a Neuronal Calcium-Myristoyl Switch Protein, Visinin-Like Protein 3.

Results show that in the presence of calcium, N-myristoylation significantly increases the kinetic rate of VILIP adsorption to the membrane.
Title: Specific interaction to PIP2 increases the kinetic rate of membrane binding of VILIPs, a subfamily of Neuronal Calcium Sensors (NCS) proteins.

HPCAL1 did not appreciably influence the ability of WT PHOX2B.
Title: Mutations that disrupt PHOXB interaction with the neuronal calcium sensor HPCAL1 impede cellular differentiation in neuroblastoma.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Identification of novel loci for Alzheimer disease and replication of CLU, PICALM, and BIN1 in Caribbean Hispanic individuals.

The following HPCAL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HPCAL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu10525 NM_002149.3
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 NM_001258359.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 NM_001258358.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 NM_001258357.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 NM_134421.2
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 XM_011510346.2
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 XM_017003953.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X11, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 XM_011510345.1
Latest version!		 Homo sapiens hippocalcin-like 1 (HPCAL1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 XM_005246160.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 XM_017003952.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 XM_011510348.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X10, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 XM_017003950.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 XM_017003951.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 XM_005246161.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 XM_011510347.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 XM_005246162.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu10525 XM_005246163.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu35197 XM_011510349.1
Latest version!		 Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X12, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu10525 XM_011510346.1 Homo sapiens hippocalcin-like 1 (HPCAL1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version NM_002149.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-11-29
Organism Homo sapiens(human)
Product hippocalcin-like protein 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI603158.1, BC017482.1, AC007249.5 and AF070616.1. On Apr 27, 2012 this sequence version replaced NM_002149.2. Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 5. All five variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.235417.1, BC017482.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002140.2
CDS385..966
Misc Feature(1)364..366
Misc Feature(2)421..921
Misc Feature(3)499..642
Misc Feature(4)order(499..501,508..510,523..528,601..603,607..609,613..615,619..621,625..627,634..636)
Misc Feature(5)577..762
Misc Feature(6)order(601..603,607..609,613..615,634..636,709..711,715..717,721..723,742..744)
Misc Feature(7)682..906
Misc Feature(8)order(709..711,715..717,721..723,742..744,853..855,859..861,865..867,886..888)
Exon (1)1..274
Exon (2)275..360
Exon (3)361..762
Exon (4)763..868
Exon (5)869..1762
Translation

Target ORF information:

RefSeq Version NM_002149.3
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_002149.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version NM_001258357.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-11-29
Organism Homo sapiens(human)
Product hippocalcin-like protein 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU145561.1, BC017482.1, AC007249.5 and AF070616.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 5. All five variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU145561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2149876 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001245286.1
CDS159..740
Misc Feature(1)138..140
Misc Feature(2)195..695
Misc Feature(3)273..416
Misc Feature(4)order(273..275,282..284,297..302,375..377,381..383,387..389,393..395,399..401,408..410)
Misc Feature(5)351..536
Misc Feature(6)order(375..377,381..383,387..389,408..410,483..485,489..491,495..497,516..518)
Misc Feature(7)456..680
Misc Feature(8)order(483..485,489..491,495..497,516..518,627..629,633..635,639..641,660..662)
Exon (1)1..48
Exon (2)49..134
Exon (3)135..536
Exon (4)537..642
Exon (5)643..1536
Translation

Target ORF information:

RefSeq Version NM_001258357.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001258357.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version NM_134421.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-11-29
Organism Homo sapiens(human)
Product hippocalcin-like protein 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK000596.1, AC007240.2, AC007249.5 and AF070616.1. On Apr 27, 2012 this sequence version replaced NM_134421.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 5. All five variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK000596.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2161836 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_602293.1
CDS527..1108
Misc Feature(1)506..508
Misc Feature(2)563..1063
Misc Feature(3)641..784
Misc Feature(4)order(641..643,650..652,665..670,743..745,749..751,755..757,761..763,767..769,776..778)
Misc Feature(5)719..904
Misc Feature(6)order(743..745,749..751,755..757,776..778,851..853,857..859,863..865,884..886)
Misc Feature(7)824..1048
Misc Feature(8)order(851..853,857..859,863..865,884..886,995..997,1001..1003,1007..1009,1028..1030)
Exon (1)1..243
Exon (2)244..329
Exon (3)330..502
Exon (4)503..904
Exon (5)905..1010
Exon (6)1011..1904
Translation

Target ORF information:

RefSeq Version NM_134421.2
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_134421.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version NM_001258358.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-11-28
Organism Homo sapiens(human)
Product hippocalcin-like protein 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI601950.1, BC017482.1, AC007249.5 and AF070616.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 5. All five variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279871.3937.1, SRR1803611.392192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001245287.1
CDS171..752
Misc Feature(1)150..152
Misc Feature(2)207..707
Misc Feature(3)285..428
Misc Feature(4)order(285..287,294..296,309..314,387..389,393..395,399..401,405..407,411..413,420..422)
Misc Feature(5)363..548
Misc Feature(6)order(387..389,393..395,399..401,420..422,495..497,501..503,507..509,528..530)
Misc Feature(7)468..692
Misc Feature(8)order(495..497,501..503,507..509,528..530,639..641,645..647,651..653,672..674)
Exon (1)1..60
Exon (2)61..146
Exon (3)147..548
Exon (4)549..654
Exon (5)655..1548
Translation

Target ORF information:

RefSeq Version NM_001258358.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001258358.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version NM_001258359.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-11-29
Organism Homo sapiens(human)
Product hippocalcin-like protein 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292280.1, BC017482.1, AC007249.5 and AF070616.1. Transcript Variant: This variant (5) represents the longest transcript. All five variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292280.1, SRR1803612.267494.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001245288.1
CDS565..1146
Misc Feature(1)544..546
Misc Feature(2)601..1101
Misc Feature(3)679..822
Misc Feature(4)order(679..681,688..690,703..708,781..783,787..789,793..795,799..801,805..807,814..816)
Misc Feature(5)757..942
Misc Feature(6)order(781..783,787..789,793..795,814..816,889..891,895..897,901..903,922..924)
Misc Feature(7)862..1086
Misc Feature(8)order(889..891,895..897,901..903,922..924,1033..1035,1039..1041,1045..1047,1066..1068)
Exon (1)1..454
Exon (2)455..540
Exon (3)541..942
Exon (4)943..1048
Exon (5)1049..1942
Translation

Target ORF information:

RefSeq Version NM_001258359.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001258359.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_017003952.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product hippocalcin-like protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016859441.1
CDS345..926
Misc Feature(1)381..881
Misc Feature(2)459..602
Misc Feature(3)order(459..461,468..470,483..488,561..563,567..569,573..575,579..581,585..587,594..596)
Misc Feature(4)537..722
Misc Feature(5)order(561..563,567..569,573..575,594..596,669..671,675..677,681..683,702..704)
Misc Feature(6)642..866
Misc Feature(7)order(669..671,675..677,681..683,702..704,813..815,819..821,825..827,846..848)
Translation

Target ORF information:

RefSeq Version XM_017003952.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017003952.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_011510346.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product hippocalcin-like protein 1 isoform X1
Comment 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508648.1
Translation

Target ORF information:

RefSeq Version XM_011510346.1
Organism Homo sapiens(Human)
Definition Homo sapiens hippocalcin-like 1 (HPCAL1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011510346.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_011510348.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product hippocalcin-like protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508650.1
CDS463..1044
Misc Feature(1)499..999
Misc Feature(2)577..720
Misc Feature(3)order(577..579,586..588,601..606,679..681,685..687,691..693,697..699,703..705,712..714)
Misc Feature(4)655..840
Misc Feature(5)order(679..681,685..687,691..693,712..714,787..789,793..795,799..801,820..822)
Misc Feature(6)760..984
Misc Feature(7)order(787..789,793..795,799..801,820..822,931..933,937..939,943..945,964..966)
Translation

Target ORF information:

RefSeq Version XM_011510348.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X10, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011510348.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_011510346.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product hippocalcin-like protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011510346.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508648.1
CDS229..810
Misc Feature(1)265..765
Misc Feature(2)343..486
Misc Feature(3)order(343..345,352..354,367..372,445..447,451..453,457..459,463..465,469..471,478..480)
Misc Feature(4)421..606
Misc Feature(5)order(445..447,451..453,457..459,478..480,553..555,559..561,565..567,586..588)
Misc Feature(6)526..750
Misc Feature(7)order(553..555,559..561,565..567,586..588,697..699,703..705,709..711,730..732)
Translation

Target ORF information:

RefSeq Version XM_011510346.2
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011510346.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_017003950.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product hippocalcin-like protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016859439.1
CDS2117..2698
Misc Feature(1)2153..2653
Misc Feature(2)2231..2374
Misc Feature(3)order(2231..2233,2240..2242,2255..2260,2333..2335,2339..2341,2345..2347,2351..2353,2357..2359,2366..2368)
Misc Feature(4)2309..2494
Misc Feature(5)order(2333..2335,2339..2341,2345..2347,2366..2368,2441..2443,2447..2449,2453..2455,2474..2476)
Misc Feature(6)2414..2638
Misc Feature(7)order(2441..2443,2447..2449,2453..2455,2474..2476,2585..2587,2591..2593,2597..2599,2618..2620)
Translation

Target ORF information:

RefSeq Version XM_017003950.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017003950.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_017003951.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product hippocalcin-like protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016859440.1
CDS600..1181
Misc Feature(1)636..1136
Misc Feature(2)714..857
Misc Feature(3)order(714..716,723..725,738..743,816..818,822..824,828..830,834..836,840..842,849..851)
Misc Feature(4)792..977
Misc Feature(5)order(816..818,822..824,828..830,849..851,924..926,930..932,936..938,957..959)
Misc Feature(6)897..1121
Misc Feature(7)order(924..926,930..932,936..938,957..959,1068..1070,1074..1076,1080..1082,1101..1103)
Translation

Target ORF information:

RefSeq Version XM_017003951.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017003951.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_005246161.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product hippocalcin-like protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005246218.1
CDS573..1154
Misc Feature(1)609..1109
Misc Feature(2)687..830
Misc Feature(3)order(687..689,696..698,711..716,789..791,795..797,801..803,807..809,813..815,822..824)
Misc Feature(4)765..950
Misc Feature(5)order(789..791,795..797,801..803,822..824,897..899,903..905,909..911,930..932)
Misc Feature(6)870..1094
Misc Feature(7)order(897..899,903..905,909..911,930..932,1041..1043,1047..1049,1053..1055,1074..1076)
Translation

Target ORF information:

RefSeq Version XM_005246161.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005246161.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_005246162.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product hippocalcin-like protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005246219.1
CDS576..1157
Misc Feature(1)612..1112
Misc Feature(2)690..833
Misc Feature(3)order(690..692,699..701,714..719,792..794,798..800,804..806,810..812,816..818,825..827)
Misc Feature(4)768..953
Misc Feature(5)order(792..794,798..800,804..806,825..827,900..902,906..908,912..914,933..935)
Misc Feature(6)873..1097
Misc Feature(7)order(900..902,906..908,912..914,933..935,1044..1046,1050..1052,1056..1058,1077..1079)
Translation

Target ORF information:

RefSeq Version XM_005246162.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005246162.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_017003953.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product hippocalcin-like protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016859442.1
CDS480..1061
Misc Feature(1)516..1016
Misc Feature(2)594..737
Misc Feature(3)order(594..596,603..605,618..623,696..698,702..704,708..710,714..716,720..722,729..731)
Misc Feature(4)672..857
Misc Feature(5)order(696..698,702..704,708..710,729..731,804..806,810..812,816..818,837..839)
Misc Feature(6)777..1001
Misc Feature(7)order(804..806,810..812,816..818,837..839,948..950,954..956,960..962,981..983)
Translation

Target ORF information:

RefSeq Version XM_017003953.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X11, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017003953.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_005246160.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product hippocalcin-like protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005246217.1
CDS450..1031
Misc Feature(1)486..986
Misc Feature(2)564..707
Misc Feature(3)order(564..566,573..575,588..593,666..668,672..674,678..680,684..686,690..692,699..701)
Misc Feature(4)642..827
Misc Feature(5)order(666..668,672..674,678..680,699..701,774..776,780..782,786..788,807..809)
Misc Feature(6)747..971
Misc Feature(7)order(774..776,780..782,786..788,807..809,918..920,924..926,930..932,951..953)
Translation

Target ORF information:

RefSeq Version XM_005246160.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005246160.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_011510347.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product hippocalcin-like protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508649.1
CDS670..1251
Misc Feature(1)706..1206
Misc Feature(2)784..927
Misc Feature(3)order(784..786,793..795,808..813,886..888,892..894,898..900,904..906,910..912,919..921)
Misc Feature(4)862..1047
Misc Feature(5)order(886..888,892..894,898..900,919..921,994..996,1000..1002,1006..1008,1027..1029)
Misc Feature(6)967..1191
Misc Feature(7)order(994..996,1000..1002,1006..1008,1027..1029,1138..1140,1144..1146,1150..1152,1171..1173)
Translation

Target ORF information:

RefSeq Version XM_011510347.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011510347.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_011510345.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product hippocalcin-like protein 1 isoform X1
Comment 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508647.1
Translation

Target ORF information:

RefSeq Version XM_011510345.1
Organism Homo sapiens(Human)
Definition Homo sapiens hippocalcin-like 1 (HPCAL1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011510345.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10525
Clone ID Related Accession (Same CDS sequence) NM_002149.3 , XM_017003952.1 , NM_001258357.1 , NM_134421.2 , XM_011510346.1 , XM_011510348.1 , NM_001258358.1 , XM_011510346.2 , XM_017003950.1 , XM_017003951.1 , XM_005246161.1 , XM_005246162.1 , XM_017003953.1 , XM_005246160.1 , XM_011510347.1 , XM_011510345.1 , XM_005246163.1 , NM_001258359.1
Accession Version XM_005246163.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 582bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product hippocalcin-like protein 1 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005246220.1
CDS623..1204
Misc Feature(1)659..1159
Misc Feature(2)737..880
Misc Feature(3)order(737..739,746..748,761..766,839..841,845..847,851..853,857..859,863..865,872..874)
Misc Feature(4)815..1000
Misc Feature(5)order(839..841,845..847,851..853,872..874,947..949,953..955,959..961,980..982)
Misc Feature(6)920..1144
Misc Feature(7)order(947..949,953..955,959..961,980..982,1091..1093,1097..1099,1103..1105,1124..1126)
Translation

Target ORF information:

RefSeq Version XM_005246163.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005246163.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGGCAAAC TGTCCTTGGA AGAATTCATC AGAGGTGCCA AGAGCGACCC CTCCATCGTC 
CGCCTGCTGC AGTGCGACCC CAGCAGTGCC AGTCAGTTCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu35197
Clone ID Related Accession (Same CDS sequence) XM_011510349.1
Accession Version XM_011510349.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 573bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(human)
Product hippocalcin-like protein 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGTGTCCC TGGGGAAGCC ACCCATGGTC ACTCGCTGCC CGAATCTGCC TCCTGGCTTG 
GCCCTGCCCA CCTCTTCAGA GGGCATCTTC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011508651.1
CDS400..972
Misc Feature(1)514..657
Misc Feature(2)order(514..516,523..525,538..543,616..618,622..624,628..630,634..636,640..642,649..651)
Misc Feature(3)517..666
Misc Feature(4)592..777
Misc Feature(5)601..774
Misc Feature(6)order(616..618,622..624,628..630,649..651,724..726,730..732,736..738,757..759)
Misc Feature(7)697..>882
Misc Feature(8)700..882
Misc Feature(9)order(724..726,730..732,736..738,757..759,868..870,874..876,880..882)
Translation

Target ORF information:

RefSeq Version XM_011510349.1
Organism Homo sapiens(human)
Definition Homo sapiens hippocalcin like 1 (HPCAL1), transcript variant X12, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011510349.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGCAAAC AGAACAGCAA GCTGCGGCCC GAGGTGCTGC AGGACCTGCG GGAGAACACG 
GAGTTCACCG ACCACGAGCT GCAGGAGTGG TACAAGGGCT TCCTCAAGGA CTGCCCCACC 
GGCCACCTGA CCGTGGACGA GTTCAAGAAG ATCTACGCCA ACTTCTTCCC CTACGGCGAC 
GCTTCCAAGT TCGCCGAGCA CGTCTTCCGC ACCTTCGACA CCAACGGCGA CGGCACCATC 
GACTTCCGGG AGTTCATCAT TGCGCTGAGC GTGACCTCGC GGGGCAAGCT GGAGCAGAAG 
CTCAAGTGGG CCTTCAGCAT GTACGACCTG GACGGCAACG GCTACATCAG CCGCAGCGAG 
ATGCTGGAGA TCGTGCAGGC CATCTACAAG ATGGTGTCGT CTGTGATGAA GATGCCGGAG 
GATGAGTCCA CCCCGGAGAA GCGCACAGAC AAGATCTTCA GGCAGATGGA CACCAACAAT 
GACGTGTCCC TGGGGAAGCC ACCCATGGTC ACTCGCTGCC CGAATCTGCC TCCTGGCTTG 
GCCCTGCCCA CCTCTTCAGA GGGCATCTTC TAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Hippocalcin-like 1 suppresses hepatocellular carcinoma progression by promoting p21(Waf/Cip1) stabilization by activating the ERK1/2-MAPK pathway.
Hepatology (Baltimore, Md.)63(3)880-97(2016 Mar)
Zhang Y,Liu Y,Duan J,Yan H,Zhang J,Zhang H,Fan Q,Luo F,Yan G,Qiao K,Liu J


book

Structure and Calcium Binding Properties of a Neuronal Calcium-Myristoyl Switch Protein, Visinin-Like Protein 3.
PloS one11(11)e0165921(2016)
Li C,Lim S,Braunewell KH,Ames JB


book

The EHEC-host interactome reveals novel targets for the translocated intimin receptor.
Scientific reports47531(2014 Dec)
Blasche S,Arens S,Ceol A,Siszler G,Schmidt MA,H?user R,Schwarz F,Wuchty S,Aloy P,Uetz P,Stradal T,Koegl M


book

Specific interaction to PIP2 increases the kinetic rate of membrane binding of VILIPs, a subfamily of Neuronal Calcium Sensors (NCS) proteins.
Biochimica et biophysica acta1838(10)2698-707(2014 Oct)
Rebaud S,Wang CK,Sarkis J,Mason L,Simon A,Blum LJ,Hofmann A,Girard-Egrot AP


book

Mutations that disrupt PHOXB interaction with the neuronal calcium sensor HPCAL1 impede cellular differentiation in neuroblastoma.
Oncogene33(25)3316-24(2014 Jun)
Wang W,Zhong Q,Teng L,Bhatnagar N,Sharma B,Zhang X,Luther W 2nd,Haynes LP,Burgoyne RD,Vidal M,Volchenboum S,Hill DE,George RE