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HPRT1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol HPRT1
Entrez Gene ID 3251
Full Name hypoxanthine phosphoribosyltransferase 1
Synonyms HGPRT, HPRT
General protein information
Preferred Names
hypoxanthine-guanine phosphoribosyltransferase
Names
hypoxanthine-guanine phosphoribosyltransferase
HGPRTase
NP_000185.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq26.1

Summary The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009]. lac of sum
Disorder MIM:

308000

Disorder Html: Lesch-Nyhan syndrome, 300322 (3); HPRT-related gout, 300323 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000194 NP_000185 hypoxanthine-guanine phosphoribosyltransferase
XM_011531328 XP_011529630 hypoxanthine-guanine phosphoribosyltransferase isoform X1



Homo sapiens (human) HPRT1 NP_000185.1
Pan troglodytes (chimpanzee) HPRT1 NP_001104287.1
Macaca mulatta (Rhesus monkey) LOC709186 XP_001097691.1
Canis lupus familiaris (dog) HPRT1 NP_001003357.1
Bos taurus (cattle) LOC510369 XP_002686486.1
Mus musculus (house mouse) Hprt NP_038584.2
Rattus norvegicus (Norway rat) Hprt1 NP_036715.1
Gallus gallus (chicken) HPRT1 NP_990179.1
Danio rerio (zebrafish) hprt1 NP_998151.1
Caenorhabditis elegans CELE_Y105E8B.5 NP_493545.1
Xenopus (Silurana) tropicalis (western clawed frog) hprt1 NP_989312.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HPRT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HPRT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu26272
NM_000194 Homo sapiens hypoxanthine phosphoribosyltransferase 1 (HPRT1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu58312 XM_011531328 PREDICTED: Homo sapiens hypoxanthine phosphoribosyltransferase 1 (HPRT1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
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5 or more Clone 50% OFF
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30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26272
Clone ID Related Accession (Same CDS sequence) NM_000194
Accession Version NM_000194.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 657bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 16-JUL-2015
Organism Homo sapiens (human)
Product hypoxanthine-guanine phosphoribosyltransferase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004383.1, M31642.1 and BC000578.2. This sequence is a reference standard in the RefSeqGene project. On Jan 9, 2008 this sequence version replaced gi:4504482. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000578.2, M31642.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGCGACCC GCAGCCCTGG CGTCGTGATT AGTGATGATG AACCAGGTTA TGACCTTGAT 
TTATTTTGCA TACCTAATCA TTATGCTGAG GATTTGGAAA GGGTGTTTAT TCCTCATGGA
CTAATTATGG ACAGGACTGA ACGTCTTGCT CGAGATGTGA TGAAGGAGAT GGGAGGCCAT
CACATTGTAG CCCTCTGTGT GCTCAAGGGG GGCTATAAAT TCTTTGCTGA CCTGCTGGAT
TACATCAAAG CACTGAATAG AAATAGTGAT AGATCCATTC CTATGACTGT AGATTTTATC
AGACTGAAGA GCTATTGTAA TGACCAGTCA ACAGGGGACA TAAAAGTAAT TGGTGGAGAT
GATCTCTCAA CTTTAACTGG AAAGAATGTC TTGATTGTGG AAGATATAAT TGACACTGGC
AAAACAATGC AGACTTTGCT TTCCTTGGTC AGGCAGTATA ATCCAAAGAT GGTCAAGGTC
GCAAGCTTGC TGGTGAAAAG GACCCCACGA AGTGTTGGAT ATAAGCCAGA CTTTGTTGGA
TTTGAAATTC CAGACAAGTT TGTTGTAGGA TATGCCCTTG ACTATAATGA ATACTTCAGG
GATTTGAATC ATGTTTGTGT CATTAGTGAA ACTGGAAAAG CAAAATACAA AGCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000185.1
CDS168..824
Misc Feature(1)171..173(+)
Misc Feature(2)306..713(+)
Misc Feature(3)369..665(+)
Misc Feature(4)474..476(+)
Exon (1)1..194
Gene:HPRT1
Gene Synonym:
Exon (2)195..301
Gene:HPRT1
Gene Synonym:
Exon (3)302..485
Gene:HPRT1
Gene Synonym:
Exon (4)486..551
Gene:HPRT1
Gene Synonym:
Exon (5)552..569
Gene:HPRT1
Gene Synonym:
Exon (6)570..652
Gene:HPRT1
Gene Synonym:
Exon (7)653..699
Gene:HPRT1
Gene Synonym:
Exon (8)700..776
Gene:HPRT1
Gene Synonym:
Exon (9)777..1415
Gene:HPRT1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000194
Organism Homo sapiens (human)
Definition Homo sapiens hypoxanthine phosphoribosyltransferase 1 (HPRT1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000194

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCGACCC GCAGCCCTGG CGTCGTGATT AGTGATGATG AACCAGGTTA TGACCTTGAT 
TTATTTTGCA TACCTAATCA TTATGCTGAG GATTTGGAAA GGGTGTTTAT TCCTCATGGA
CTAATTATGG ACAGGACTGA ACGTCTTGCT CGAGATGTGA TGAAGGAGAT GGGAGGCCAT
CACATTGTAG CCCTCTGTGT GCTCAAGGGG GGCTATAAAT TCTTTGCTGA CCTGCTGGAT
TACATCAAAG CACTGAATAG AAATAGTGAT AGATCCATTC CTATGACTGT AGATTTTATC
AGACTGAAGA GCTATTGTAA TGACCAGTCA ACAGGGGACA TAAAAGTAAT TGGTGGAGAT
GATCTCTCAA CTTTAACTGG AAAGAATGTC TTGATTGTGG AAGATATAAT TGACACTGGC
AAAACAATGC AGACTTTGCT TTCCTTGGTC AGGCAGTATA ATCCAAAGAT GGTCAAGGTC
GCAAGCTTGC TGGTGAAAAG GACCCCACGA AGTGTTGGAT ATAAGCCAGA CTTTGTTGGA
TTTGAAATTC CAGACAAGTT TGTTGTAGGA TATGCCCTTG ACTATAATGA ATACTTCAGG
GATTTGAATC ATGTTTGTGT CATTAGTGAA ACTGGAAAAG CAAAATACAA AGCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu58312
Clone ID Related Accession (Same CDS sequence) XM_011531328
Accession Version XM_011531328.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 675bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product hypoxanthine-guanine phosphoribosyltransferase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011786.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGCCAAAGC CTGCAAGATA CAGGAAGCTG CCAGGCCACC AGTTGATTAG TGATGATGAA 
CCAGGTTATG ACCTTGATTT ATTTTGCATA CCTAATCATT ATGCTGAGGA TTTGGAAAGG
GTGTTTATTC CTCATGGACT AATTATGGAC AGGACTGAAC GTCTTGCTCG AGATGTGATG
AAGGAGATGG GAGGCCATCA CATTGTAGCC CTCTGTGTGC TCAAGGGGGG CTATAAATTC
TTTGCTGACC TGCTGGATTA CATCAAAGCA CTGAATAGAA ATAGTGATAG ATCCATTCCT
ATGACTGTAG ATTTTATCAG ACTGAAGAGC TATTGTAATG ACCAGTCAAC AGGGGACATA
AAAGTAATTG GTGGAGATGA TCTCTCAACT TTAACTGGAA AGAATGTCTT GATTGTGGAA
GATATAATTG ACACTGGCAA AACAATGCAG ACTTTGCTTT CCTTGGTCAG GCAGTATAAT
CCAAAGATGG TCAAGGTCGC AAGCTTGCTG GTGAAAAGGA CCCCACGAAG TGTTGGATAT
AAGCCAGACT TTGTTGGATT TGAAATTCCA GACAAGTTTG TTGTAGGATA TGCCCTTGAC
TATAATGAAT ACTTCAGGGA TTTGAATCAT GTTTGTGTCA TTAGTGAAAC TGGAAAAGCA
AAATACAAAG CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529630.1
CDS109..783
Misc Feature(1)265..672(+)
Misc Feature(2)328..624(+)
Translation

Target ORF information:

RefSeq Version XM_011531328
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens hypoxanthine phosphoribosyltransferase 1 (HPRT1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531328

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGCCAAAGC CTGCAAGATA CAGGAAGCTG CCAGGCCACC AGTTGATTAG TGATGATGAA 
CCAGGTTATG ACCTTGATTT ATTTTGCATA CCTAATCATT ATGCTGAGGA TTTGGAAAGG
GTGTTTATTC CTCATGGACT AATTATGGAC AGGACTGAAC GTCTTGCTCG AGATGTGATG
AAGGAGATGG GAGGCCATCA CATTGTAGCC CTCTGTGTGC TCAAGGGGGG CTATAAATTC
TTTGCTGACC TGCTGGATTA CATCAAAGCA CTGAATAGAA ATAGTGATAG ATCCATTCCT
ATGACTGTAG ATTTTATCAG ACTGAAGAGC TATTGTAATG ACCAGTCAAC AGGGGACATA
AAAGTAATTG GTGGAGATGA TCTCTCAACT TTAACTGGAA AGAATGTCTT GATTGTGGAA
GATATAATTG ACACTGGCAA AACAATGCAG ACTTTGCTTT CCTTGGTCAG GCAGTATAAT
CCAAAGATGG TCAAGGTCGC AAGCTTGCTG GTGAAAAGGA CCCCACGAAG TGTTGGATAT
AAGCCAGACT TTGTTGGATT TGAAATTCCA GACAAGTTTG TTGTAGGATA TGCCCTTGAC
TATAATGAAT ACTTCAGGGA TTTGAATCAT GTTTGTGTCA TTAGTGAAAC TGGAAAAGCA
AAATACAAAG CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Pseudogene-free amplification of HPRT1 in quantitative reverse transcriptase polymerase chain reaction
Anal. Biochem. 485, 46-48 (2015)
Valadan R, Amjadi O, Tehrani M, Rafiei A, Hedayatizadeh-Omran A and Alizadeh-Navaei R.


book

A proteome-scale map of the human interactome network
Cell 159 (5), 1212-1226 (2014)
Rolland T, Tasan M, Charloteaux B, Pevzner SJ, Zhong Q, Sahni N, Yi S, Lemmens I, Fontanillo C, Mosca R, Kamburov A, Ghiassian SD, Yang X, Ghamsari L, Balcha D, Begg BE, Braun P, Brehme M, Broly MP, Carvunis AR, Convery-Zupan D, Corominas R, Coulombe-Huntington J, Dann E, Dreze M, Dricot A, Fan C, Franzosa E, Gebreab F, Gutierrez BJ, Hardy MF, Jin M, Kang S, Kiros R, Lin GN, Luck K, MacWilliams A, Menche J, Murray RR, Palagi A, Poulin MM, Rambout X, Rasla J, Reichert P, Romero V, Ruyssinck E, Sahalie JM, Scholz A, Shah AA, Sharma A, Shen Y, Spirohn K, Tam S, Tejeda AO, Trigg SA, Twizere JC, Vega K, Walsh J, Cusick ME, Xia Y, Barabasi AL, Iakoucheva LM, Aloy P, De Las Rivas J, Tavernier J, Calderwood MA, Hill DE, Hao T, Roth FP and Vidal M.


book

Novel hypoxanthine guanine phosphoribosyltransferase gene mutations in Saudi Arabian hyperuricemia patients
Biomed Res Int 2014, 290325 (2014)
Alanazi M, Al-Arfaj AS, Abduljaleel Z, Fahad Al-Arfaj H, Reddy Parine N, Purusottapatnam Shaik J, Khan Z and Ali Khan Pathan A.


book

Hypoxanthine guanine phosphoribosyltransferase (HPRT) deficiencies: HPRT1 mutations in new Japanese families and PRPP concentration
Nucleosides Nucleotides Nucleic Acids 33 (4-6), 218-222 (2014)
Yamada Y, Nomura N, Yamada K, Kimura R, Fukushi D, Wakamatsu N, Matsuda Y, Yamauchi T, Ueda T, Hasegawa H, Nakamura M, Ichida K, Kaneko K and Fujimori S.


book

Dystonia Overview
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Klein,C., Marras,C. and Munchau,A.


book

Lesch-Nyhan Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Wallace SE, Amemiya A, Bean LJH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Nyhan,W.L., O'Neill,J.P., Jinnah,H.A. and Harris,J.C.


book

A review of the molecular basis of hypoxanthine-guanine phosphoribosyltransferase (HPRT) deficiency
Hum. Genet. 90 (3), 195-207 (1992)
Sculley DG, Dawson PA, Emmerson BT and Gordon RB.


book

Characterization of mutations in phenotypic variants of hypoxanthine phosphoribosyltransferase deficiency
Hum. Mol. Genet. 1 (6), 427-432 (1992)
Sege-Peterson K, Chambers J, Page T, Jones OW and Nyhan WL.


book

The point mutation of hypoxanthine-guanine phosphoribosyltransferase (HPRTEdinburgh) and detection by allele-specific polymerase chain reaction
Hum. Genet. 88 (6), 695-696 (1992)
Lightfoot T, Joshi R, Nuki G and Snyder FF.


book

Identification of two independent Japanese mutant HPRT genes using the PCR technique
Adv. Exp. Med. Biol. 309B, 121-124 (1991)
Yamada Y, Goto H and Ogasawara N.


 
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