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HSPA8 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol HSPA8
Entrez Gene ID 3312
Full Name heat shock protein family A (Hsp70) member 8
Synonyms HEL-33,HEL-S-72p,HSC54,HSC70,HSC71,HSP71,HSP73,HSPA10,LAP-1,LAP1,NIP71
General protein information
Preferred Names

heat shock protein family A (Hsp70) member 8

Names

heat shock cognate 71 kDa protein
LPS-associated protein 1
N-myristoyltransferase inhibitor protein 71
constitutive heat shock protein 70
epididymis luminal protein 33
epididymis secretory sperm binding protein Li 72p
heat shock 70kDa protein 8
heat shock 70kd protein 10
heat shock cognate protein 54
lipopolysaccharide-associated protein 1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

11

11q24.1

Summary This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011].
Disorder MIM:

600816


mRNA and Protein(s)

mRNA Protein Name
NM_153201.3 NP_694881.1 heat shock cognate 71 kDa protein isoform 2
XM_011542798.1 XP_011541100.1 heat shock cognate 71 kDa protein isoform X1
NM_006597.5 NP_006588.1 heat shock cognate 71 kDa protein isoform 1



Homo sapiens (human) HSPA8 NP_006588.1
Canis lupus familiaris (dog) HSPA8 XP_005619707.1
Bos taurus (cattle) HSPA8 NP_776770.2
Rattus norvegicus (Norway rat) Hspa8 NP_077327.1
Gallus gallus (chicken) HSPA8 NP_990334.1
Caenorhabditis elegans (roundworm) hsp-1 NP_503068.1
Macaca mulatta (rhesus monkey) HSPA8 XP_002799868.1
Mus musculus (house mouse) Hspa8 NP_112442.2
Danio rerio (zebrafish) hspa8 NP_001103873.1
Xenopus tropicalis (tropical clawed frog) hspa8 XP_004916187.1
Pan troglodytes (chimpanzee) HSPA8 XP_003313441.2
Drosophila melanogaster (fruit fly) Hsc70-1 NP_524063.1
Arabidopsis thaliana (thale cress) AT3G09440 NP_001189847.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004944 XP_315042.3


Related articles in PubMed

Tetrameric Assembly of K
Li K, Jiang Q, Bai X, Yang YF, Ruan MY, Cai SQ
Molecular cell65(1)52-65(2017 Jan)

ARD1-mediated Hsp70 acetylation balances stress-induced protein refolding and degradation.
Seo JH, Park JH, Lee EJ, Vo TT, Choi H, Kim JY, Jang JK, Wee HJ, Lee HS, Jang SH, Park ZY, Jeong J, Lee KJ, Seok SH, Park JY, Lee BJ, Lee MN, Oh GT, Kim KW
Nature communications712882(2016 Oct)

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.
Sellier C, Campanari ML, Julie Corbier C, Gaucherot A, Kolb-Cheynel I, Oulad-Abdelghani M, Ruffenach F, Page A, Ciura S, Kabashi E, Charlet-Berguerand N
The EMBO journal35(12)1276-97(2016 Jun)

Salivary protein histatin 3 regulates cell proliferation by enhancing p27(Kip1) and heat shock cognate protein 70 ubiquitination.
Imamura Y, Wang PL, Masuno K, Sogawa N
Biochemical and biophysical research communications470(2)269-274(2016 Feb)

Genetic mutations strengthen functional association of LAP1 with DYT1 dystonia and muscular dystrophy.
Rebelo S, da Cruz E Silva EF, da Cruz E Silva OA
Mutation research. Reviews in mutation research76642-7(2015 Oct-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study demonstrates a critical role of Hsc70 in SV40 endoplasmic reticulum-to-cytosol penetration and reveal how SGTA controls Hsc70 to impact this process
Title: SGTA-Dependent Regulation of Hsc70 Promotes Cytosol Entry of Simian Virus 40 from the Endoplasmic Reticulum.

HSPA1A and HSPA8 have roles in parturition through stimulating immune inflammatory and estrogen response
Title: Functional analysis of HSPA1A and HSPA8 in parturition.

These results suggest that Bag1 and Bag3 control the stability of the Hsc70-client complex using at least two distinct protein-protein contacts, providing a previously under-appreciated layer of molecular regulation in the human Hsc70 system.
Title: Non-canonical Interactions between Heat Shock Cognate Protein 70 (Hsc70) and Bcl2-associated Anthanogene (BAG) Co-Chaperones Are Important for Client Release.

In fact, DnaJC5 overexpression induced tau release in cells, neurons, and brain tissue, but only when activity of the chaperone Hsc70 was intact and when tau was able to associate with this chaperone.
Title: DnaJ/Hsc70 chaperone complexes control the extracellular release of neurodegenerative-associated proteins.

These findings provide further evidence that histatin 3 may be involved in the regulation of cell proliferation, particularly during G1/S transition, via the ubiquitin-proteasome system of p27(Kip1) and HSC70.
Title: Salivary protein histatin 3 regulates cell proliferation by enhancing p27(Kip1) and heat shock cognate protein 70 ubiquitination.

The following HSPA8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPA8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu27538 NM_006597.5
Latest version!
Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu27538 XM_011542798.1
Latest version!
Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu27531 NM_153201.3
Latest version!
Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27538
Clone ID Related Accession (Same CDS sequence) XM_011542798.1 , NM_006597.5
Accession Version NM_006597.5 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product heat shock cognate 71 kDa protein isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC312904.1, DC395565.1, BC019816.2 and AK222628.1. This sequence is a reference standard in the RefSeqGene project. On Jul 30, 2013 this sequence version replaced NM_006597.4. Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ224294.1, SRR1803612.77063.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_006588.1
CDS278..2218
Misc Feature(1)170..172
Misc Feature(2)278..2116
Misc Feature(3)281..1435
Misc Feature(4)281..283
Misc Feature(5)293..1420
Misc Feature(6)order(305..307,311..322,488..490,716..718,800..802,872..889,893..895,965..970,1079..1081,1088..1093,1100..1102,1289..1297,1301..1306,1373..1375)
Misc Feature(7)order(344..346,350..352,356..358,371..379,383..385,425..427,437..439,446..448,674..679,1049..1051,1061..1063,1082..1084,1091..1096,1103..1105,1118..1120,1124..1126,1130..1135,1145..1147,1301..1303)
Misc Feature(8)order(374..376,380..382,446..448,455..460,1046..1051,1058..1063,1070..1075,1082..1084,1124..1135,1151..1153,1157..1159)
Misc Feature(9)599..601
Misc Feature(10)order(731..733,851..853,914..916,920..928,932..937,1250..1252)
Misc Feature(11)734..736
Misc Feature(12)734..736
Misc Feature(13)833..1408
Misc Feature(14)1013..1015
Misc Feature(15)1232..1234
Misc Feature(16)1232..1234
Misc Feature(17)1259..1261
Misc Feature(18)1262..1264
Misc Feature(19)1361..1363
Misc Feature(20)1457..1804
Misc Feature(21)1682..1684
Misc Feature(22)1706..1708
Misc Feature(23)1811..1813
Misc Feature(24)1811..1813
Misc Feature(25)1847..1849
Misc Feature(26)1898..1900
Misc Feature(27)1958..1960
Misc Feature(28)2042..2044
Misc Feature(29)2066..2068
Misc Feature(30)2078..2080
Exon (1)1..272
Exon (2)273..482
Exon (3)483..688
Exon (4)689..841
Exon (5)842..1397
Exon (6)1398..1600
Exon (7)1601..1799
Exon (8)1800..2032
Exon (9)2033..2460
Translation

Target ORF information:

RefSeq Version NM_006597.5
Organism Homo sapiens(Human)
Definition Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_006597.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27538
Clone ID Related Accession (Same CDS sequence) XM_011542798.1 , NM_006597.5
Accession Version XM_011542798.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product heat shock cognate 71 kDa protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011541100.1
CDS26..1966
Misc Feature(1)26..1864
Misc Feature(2)41..1168
Misc Feature(3)order(53..55,59..70,236..238,464..466,548..550,620..637,641..643,713..718,827..829,836..841,848..850,1037..1045,1049..1054,1121..1123)
Misc Feature(4)order(92..94,98..100,104..106,119..127,131..133,173..175,185..187,194..196,422..427,797..799,809..811,830..832,839..844,851..853,866..868,872..874,878..883,893..895,1049..1051)
Misc Feature(5)order(122..124,128..130,194..196,203..208,794..799,806..811,818..823,830..832,872..883,899..901,905..907)
Misc Feature(6)order(479..481,599..601,662..664,668..676,680..685,998..1000)
Translation

Target ORF information:

RefSeq Version XM_011542798.1
Organism Homo sapiens(Human)
Definition Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011542798.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TACCTCCTGC ACCCCGAGGT GTTCCTCAGA TTGAAGTCAC TTTTGACATT
GATGCCAATG GTATACTCAA TGTCTCTGCT GTGGACAAGA GTACGGGAAA AGAGAACAAG
ATTACTATCA CTAATGACAA GGGCCGTTTG AGCAAGGAAG ACATTGAACG TATGGTCCAG
GAAGCTGAGA AGTACAAAGC TGAAGATGAG AAGCAGAGGG ACAAGGTGTC ATCCAAGAAT
TCACTTGAGT CCTATGCCTT CAACATGAAA GCAACTGTTG AAGATGAGAA ACTTCAAGGC
AAGATTAACG ATGAGGACAA ACAGAAGATT CTGGACAAGT GTAATGAAAT TATCAACTGG
CTTGATAAGA ATCAGACTGC TGAGAAGGAA GAATTTGAAC ATCAACAGAA AGAGCTGGAG
AAAGTTTGCA ACCCCATCAT CACCAAGCTG TACCAGAGTG CAGGAGGCAT GCCAGGAGGA
ATGCCTGGGG GATTTCCTGG TGGTGGAGCT CCTCCCTCTG GTGGTGCTTC CTCAGGGCCC
ACCATTGAAG AGGTTGATTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27531
Clone ID Related Accession (Same CDS sequence) NM_153201.3
Accession Version NM_153201.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-30
Organism Homo sapiens(Human)
Product heat shock cognate 71 kDa protein isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC312904.1, DC395565.1, AB034951.1 and AK222628.1. On or before Aug 31, 2013 this sequence version replaced XM_005271538.1, NM_153201.2. Transcript Variant: This variant (2) uses alternate splice sites, and is missing an exon in the 3' coding region compared to variant 1. However, it maintains the reading frame, and encodes a shorter isoform (2) missing an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK222628.1, SRR1163657.50984.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TGCCAGGAGG AATGCCTGGG GGATTTCCTG GTGGTGGAGC TCCTCCCTCT
GGTGGTGCTT CCTCAGGGCC CACCATTGAA GAGGTTGATT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_694881.1
CDS278..1759
Misc Feature(1)170..172
Misc Feature(2)281..1435
Misc Feature(3)281..283
Misc Feature(4)293..1420
Misc Feature(5)296..1396
Misc Feature(6)order(305..307,311..322,488..490,716..718,800..802,872..889,893..895,965..970,1079..1081,1088..1093,1100..1102,1289..1297,1301..1306,1373..1375)
Misc Feature(7)order(344..346,350..352,356..358,371..379,383..385,425..427,437..439,446..448,674..679,1049..1051,1061..1063,1082..1084,1091..1096,1103..1105,1118..1120,1124..1126,1130..1135,1145..1147,1301..1303)
Misc Feature(8)order(374..376,380..382,446..448,455..460,1046..1051,1058..1063,1070..1075,1082..1084,1124..1135,1151..1153,1157..1159)
Misc Feature(9)599..601
Misc Feature(10)order(731..733,851..853,914..916,920..928,932..937,1250..1252)
Misc Feature(11)734..736
Misc Feature(12)734..736
Misc Feature(13)833..1408
Misc Feature(14)1013..1015
Misc Feature(15)1232..1234
Misc Feature(16)1232..1234
Misc Feature(17)1259..1261
Misc Feature(18)1262..1264
Misc Feature(19)1361..1363
Misc Feature(20)1685..1687
Exon (1)1..272
Exon (2)273..482
Exon (3)483..688
Exon (4)689..841
Exon (5)842..1397
Exon (6)1398..1600
Exon (7)1601..1664
Exon (8)1665..2001
Translation

Target ORF information:

RefSeq Version NM_153201.3
Organism Homo sapiens(Human)
Definition Homo sapiens heat shock protein family A (Hsp70) member 8 (HSPA8), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_153201.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGTCCAAGG GACCTGCAGT TGGTATTGAT CTTGGCACCA CCTACTCTTG TGTGGGTGTT 
TTCCAGCACG GAAAAGTCGA GATAATTGCC AATGATCAGG GAAACCGAAC CACTCCAAGC
TATGTCGCCT TTACGGACAC TGAACGGTTG ATCGGTGATG CCGCAAAGAA TCAAGTTGCA
ATGAACCCCA CCAACACAGT TTTTGATGCC AAACGTCTGA TTGGACGCAG ATTTGATGAT
GCTGTTGTCC AGTCTGATAT GAAACATTGG CCCTTTATGG TGGTGAATGA TGCTGGCAGG
CCCAAGGTCC AAGTAGAATA CAAGGGAGAG ACCAAAAGCT TCTATCCAGA GGAGGTGTCT
TCTATGGTTC TGACAAAGAT GAAGGAAATT GCAGAAGCCT ACCTTGGGAA GACTGTTACC
AATGCTGTGG TCACAGTGCC AGCTTACTTT AATGACTCTC AGCGTCAGGC TACCAAAGAT
GCTGGAACTA TTGCTGGTCT CAATGTACTT AGAATTATTA ATGAGCCAAC TGCTGCTGCT
ATTGCTTACG GCTTAGACAA AAAGGTTGGA GCAGAAAGAA ACGTGCTCAT CTTTGACCTG
GGAGGTGGCA CTTTTGATGT GTCAATCCTC ACTATTGAGG ATGGAATCTT TGAGGTCAAG
TCTACAGCTG GAGACACCCA CTTGGGTGGA GAAGATTTTG ACAACCGAAT GGTCAACCAT
TTTATTGCTG AGTTTAAGCG CAAGCATAAG AAGGACATCA GTGAGAACAA GAGAGCTGTA
AGACGCCTCC GTACTGCTTG TGAACGTGCT AAGCGTACCC TCTCTTCCAG CACCCAGGCC
AGTATTGAGA TCGATTCTCT CTATGAAGGA ATCGACTTCT ATACCTCCAT TACCCGTGCC
CGATTTGAAG AACTGAATGC TGACCTGTTC CGTGGCACCC TGGACCCAGT AGAGAAAGCC
CTTCGAGATG CCAAACTAGA CAAGTCACAG ATTCATGATA TTGTCCTGGT TGGTGGTTCT
ACTCGTATCC CCAAGATTCA GAAGCTTCTC CAAGACTTCT TCAATGGAAA AGAACTGAAT
AAGAGCATCA ACCCTGATGA AGCTGTTGCT TATGGTGCAG CTGTCCAGGC AGCCATCTTG
TCTGGAGACA AGTCTGAGAA TGTTCAAGAT TTGCTGCTCT TGGATGTCAC TCCTCTTTCC
CTTGGTATTG AAACTGCTGG TGGAGTCATG ACTGTCCTCA TCAAGCGTAA TACCACCATT
CCTACCAAGC AGACACAGAC CTTCACTACC TATTCTGACA ACCAGCCTGG TGTGCTTATT
CAGGTTTATG AAGGCGAGCG TGCCATGACA AAGGATAACA ACCTGCTTGG CAAGTTTGAA
CTCACAGGCA TGCCAGGAGG AATGCCTGGG GGATTTCCTG GTGGTGGAGC TCCTCCCTCT
GGTGGTGCTT CCTCAGGGCC CACCATTGAA GAGGTTGATT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Tetrameric Assembly of K
Molecular cell65(1)52-65(2017 Jan)
Li K,Jiang Q,Bai X,Yang YF,Ruan MY,Cai SQ


book

ARD1-mediated Hsp70 acetylation balances stress-induced protein refolding and degradation.
Nature communications712882(2016 Oct)
Seo JH,Park JH,Lee EJ,Vo TT,Choi H,Kim JY,Jang JK,Wee HJ,Lee HS,Jang SH,Park ZY,Jeong J,Lee KJ,Seok SH,Park JY,Lee BJ,Lee MN,Oh GT,Kim KW


book

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.
The EMBO journal35(12)1276-97(2016 Jun)
Sellier C,Campanari ML,Julie Corbier C,Gaucherot A,Kolb-Cheynel I,Oulad-Abdelghani M,Ruffenach F,Page A,Ciura S,Kabashi E,Charlet-Berguerand N


book

Salivary protein histatin 3 regulates cell proliferation by enhancing p27(Kip1) and heat shock cognate protein 70 ubiquitination.
Biochemical and biophysical research communications470(2)269-274(2016 Feb)
Imamura Y,Wang PL,Masuno K,Sogawa N


book

Genetic mutations strengthen functional association of LAP1 with DYT1 dystonia and muscular dystrophy.
Mutation research. Reviews in mutation research76642-7(2015 Oct-Dec)
Rebelo S,da Cruz E Silva EF,da Cruz E Silva OA


 
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