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IDH1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol IDH1
Entrez Gene ID 3417
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Synonyms HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD
General protein information
Preferred Names
isocitrate dehydrogenase [NADP] cytoplasmic
Names
isocitrate dehydrogenase [NADP] cytoplasmic
NADP(+)-specific ICDH
oxalosuccinate decarboxylase
NADP-dependent isocitrate dehydrogenase, cytosolic
NADP-dependent isocitrate dehydrogenase, peroxisomal
NP_005887.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

2

2q33.3

Summary Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]. lac of sum
Disorder MIM:

147700


mRNA and Protein(s)

mRNA Protein Name
NM_005896 NP_005887 isocitrate dehydrogenase [NADP] cytoplasmic
NM_001282387 NP_001269316 isocitrate dehydrogenase [NADP] cytoplasmic
NM_001282386 NP_001269315 isocitrate dehydrogenase [NADP] cytoplasmic



Homo sapiens (human) IDH1 NP_005887.2
Pan troglodytes (chimpanzee) IDH1 XP_001141655.1
Macaca mulatta (Rhesus monkey) IDH1 XP_001107934.1
Canis lupus familiaris (dog) IDH1 XP_536047.2
Bos taurus (cattle) IDH1 NP_851355.2
Mus musculus (house mouse) Idh1 NP_034627.3
Rattus norvegicus (Norway rat) Idh1 NP_113698.1
Gallus gallus (chicken) IDH1 XP_004942738.1
Danio rerio (zebrafish) idh1 NP_958907.1
Drosophila melanogaster (fruit fly) Idh NP_652044.1
Caenorhabditis elegans idh-1 NP_001255392.1
Arabidopsis thaliana (thale cress) cICDH NP_176768.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following IDH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IDH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu21156
NM_005896 Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu21156
NM_001282387 Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu21156
NM_001282386 Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu21156
Clone ID Related Accession (Same CDS sequence) NM_001282386 , NM_001282387 , NM_005896
Accession Version NM_005896.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1245bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product isocitrate dehydrogenase [NADP] cytoplasmic
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA728868.1, BC021046.1 and N21575.1. This sequence is a reference standard in the RefSeqGene project. On Sep 5, 2013 this sequence version replaced gi:28178824. Transcript Variant: This variant (1) and variants 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF020038.1, BC093020.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCCAAAA AAATCAGTGG CGGTTCTGTG GTAGAGATGC AAGGAGATGA AATGACACGA 
ATCATTTGGG AATTGATTAA AGAGAAACTC ATTTTTCCCT ACGTGGAATT GGATCTACAT
AGCTATGATT TAGGCATAGA GAATCGTGAT GCCACCAACG ACCAAGTCAC CAAGGATGCT
GCAGAAGCTA TAAAGAAGCA TAATGTTGGC GTCAAATGTG CCACTATCAC TCCTGATGAG
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCAAATGG CACCATACGA
AATATTCTGG GTGGCACGGT CTTCAGAGAA GCCATTATCT GCAAAAATAT CCCCCGGCTT
GTGAGTGGAT GGGTAAAACC TATCATCATA GGTCGTCATG CTTATGGGGA TCAATACAGA
GCAACTGATT TTGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAGTGAC
GGAACCCAAA AGGTGACATA CCTGGTACAT AACTTTGAAG AAGGTGGTGG TGTTGCCATG
GGGATGTATA ATCAAGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT
CTGTCTAAGG GTTGGCCTTT GTATCTGAGC ACCAAAAACA CTATTCTGAA GAAATATGAT
GGGCGTTTTA AAGACATCTT TCAGGAGATA TATGACAAGC AGTACAAGTC CCAGTTTGAA
GCTCAAAAGA TCTGGTATGA GCATAGGCTC ATCGACGACA TGGTGGCCCA AGCTATGAAA
TCAGAGGGAG GCTTCATCTG GGCCTGTAAA AACTATGATG GTGACGTGCA GTCGGACTCT
GTGGCCCAAG GGTATGGCTC TCTCGGCATG ATGACCAGCG TGCTGGTTTG TCCAGATGGC
AAGACAGTAG AAGCAGAGGC TGCCCACGGG ACTGTAACCC GTCACTACCG CATGTACCAG
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGAC CAGAGGGTTA
GCCCACAGAG CAAAGCTTGA TAACAATAAA GAGCTTGCCT TCTTTGCAAA TGCTTTGGAA
GAAGTCTCTA TTGAGACAAT TGAGGCTGGC TTCATGACCA AGGACTTGGC TGCTTGCATT
AAAGGTTTAC CCAATGTGCA ACGTTCTGAC TACTTGAATA CATTTGAGTT CATGGATAAA
CTTGGAGAAA ACTTGAAGAT CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005887.2
CDS296..1540
Misc Feature(1)257..259(+)
Misc Feature(2)296..1528(+)
Misc Feature(3)299..301(+)
Misc Feature(4)575..595(+)
Misc Feature(5)710..712(+)
Misc Feature(6)929..931(+)
Misc Feature(7)1256..1258(+)
Exon (1)1..205
Gene:IDH1
Gene Synonym:
Exon (2)206..279
Gene:IDH1
Gene Synonym:
Exon (3)280..417
Gene:IDH1
Gene Synonym:
Exon (4)418..709
Gene:IDH1
Gene Synonym:
Exon (5)710..815
Gene:IDH1
Gene Synonym:
Exon (6)816..993
Gene:IDH1
Gene Synonym:
Exon (7)994..1145
Gene:IDH1
Gene Synonym:
Exon (8)1146..1286
Gene:IDH1
Gene Synonym:
Exon (9)1287..1449
Gene:IDH1
Gene Synonym:
Exon (10)1450..2392
Gene:IDH1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005896
Organism Homo sapiens (human)
Definition Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005896

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCCAAAA AAATCAGTGG CGGTTCTGTG GTAGAGATGC AAGGAGATGA AATGACACGA 
ATCATTTGGG AATTGATTAA AGAGAAACTC ATTTTTCCCT ACGTGGAATT GGATCTACAT
AGCTATGATT TAGGCATAGA GAATCGTGAT GCCACCAACG ACCAAGTCAC CAAGGATGCT
GCAGAAGCTA TAAAGAAGCA TAATGTTGGC GTCAAATGTG CCACTATCAC TCCTGATGAG
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCAAATGG CACCATACGA
AATATTCTGG GTGGCACGGT CTTCAGAGAA GCCATTATCT GCAAAAATAT CCCCCGGCTT
GTGAGTGGAT GGGTAAAACC TATCATCATA GGTCGTCATG CTTATGGGGA TCAATACAGA
GCAACTGATT TTGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAGTGAC
GGAACCCAAA AGGTGACATA CCTGGTACAT AACTTTGAAG AAGGTGGTGG TGTTGCCATG
GGGATGTATA ATCAAGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT
CTGTCTAAGG GTTGGCCTTT GTATCTGAGC ACCAAAAACA CTATTCTGAA GAAATATGAT
GGGCGTTTTA AAGACATCTT TCAGGAGATA TATGACAAGC AGTACAAGTC CCAGTTTGAA
GCTCAAAAGA TCTGGTATGA GCATAGGCTC ATCGACGACA TGGTGGCCCA AGCTATGAAA
TCAGAGGGAG GCTTCATCTG GGCCTGTAAA AACTATGATG GTGACGTGCA GTCGGACTCT
GTGGCCCAAG GGTATGGCTC TCTCGGCATG ATGACCAGCG TGCTGGTTTG TCCAGATGGC
AAGACAGTAG AAGCAGAGGC TGCCCACGGG ACTGTAACCC GTCACTACCG CATGTACCAG
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGAC CAGAGGGTTA
GCCCACAGAG CAAAGCTTGA TAACAATAAA GAGCTTGCCT TCTTTGCAAA TGCTTTGGAA
GAAGTCTCTA TTGAGACAAT TGAGGCTGGC TTCATGACCA AGGACTTGGC TGCTTGCATT
AAAGGTTTAC CCAATGTGCA ACGTTCTGAC TACTTGAATA CATTTGAGTT CATGGATAAA
CTTGGAGAAA ACTTGAAGAT CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21156
Clone ID Related Accession (Same CDS sequence) NM_001282386 , NM_001282387 , NM_005896
Accession Version NM_001282387.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1245bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product isocitrate dehydrogenase [NADP] cytoplasmic
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR158630.1, BC012846.1 and N21575.1. This sequence is a reference standard in the RefSeqGene project. On Sep 5, 2013 this sequence version replaced gi:530370286. Transcript Variant: This variant (3) has an alternate 5' UTR exon, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012846.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCCAAAA AAATCAGTGG CGGTTCTGTG GTAGAGATGC AAGGAGATGA AATGACACGA 
ATCATTTGGG AATTGATTAA AGAGAAACTC ATTTTTCCCT ACGTGGAATT GGATCTACAT
AGCTATGATT TAGGCATAGA GAATCGTGAT GCCACCAACG ACCAAGTCAC CAAGGATGCT
GCAGAAGCTA TAAAGAAGCA TAATGTTGGC GTCAAATGTG CCACTATCAC TCCTGATGAG
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCAAATGG CACCATACGA
AATATTCTGG GTGGCACGGT CTTCAGAGAA GCCATTATCT GCAAAAATAT CCCCCGGCTT
GTGAGTGGAT GGGTAAAACC TATCATCATA GGTCGTCATG CTTATGGGGA TCAATACAGA
GCAACTGATT TTGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAGTGAC
GGAACCCAAA AGGTGACATA CCTGGTACAT AACTTTGAAG AAGGTGGTGG TGTTGCCATG
GGGATGTATA ATCAAGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT
CTGTCTAAGG GTTGGCCTTT GTATCTGAGC ACCAAAAACA CTATTCTGAA GAAATATGAT
GGGCGTTTTA AAGACATCTT TCAGGAGATA TATGACAAGC AGTACAAGTC CCAGTTTGAA
GCTCAAAAGA TCTGGTATGA GCATAGGCTC ATCGACGACA TGGTGGCCCA AGCTATGAAA
TCAGAGGGAG GCTTCATCTG GGCCTGTAAA AACTATGATG GTGACGTGCA GTCGGACTCT
GTGGCCCAAG GGTATGGCTC TCTCGGCATG ATGACCAGCG TGCTGGTTTG TCCAGATGGC
AAGACAGTAG AAGCAGAGGC TGCCCACGGG ACTGTAACCC GTCACTACCG CATGTACCAG
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGAC CAGAGGGTTA
GCCCACAGAG CAAAGCTTGA TAACAATAAA GAGCTTGCCT TCTTTGCAAA TGCTTTGGAA
GAAGTCTCTA TTGAGACAAT TGAGGCTGGC TTCATGACCA AGGACTTGGC TGCTTGCATT
AAAGGTTTAC CCAATGTGCA ACGTTCTGAC TACTTGAATA CATTTGAGTT CATGGATAAA
CTTGGAGAAA ACTTGAAGAT CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269316.1
CDS364..1608
Misc Feature(1)325..327(+)
Misc Feature(2)364..1596(+)
Misc Feature(3)367..369(+)
Misc Feature(4)643..663(+)
Misc Feature(5)778..780(+)
Misc Feature(6)997..999(+)
Misc Feature(7)1324..1326(+)
Exon (1)1..273
Gene:IDH1
Gene Synonym:
Exon (2)274..347
Gene:IDH1
Gene Synonym:
Exon (3)348..485
Gene:IDH1
Gene Synonym:
Exon (4)486..777
Gene:IDH1
Gene Synonym:
Exon (5)778..883
Gene:IDH1
Gene Synonym:
Exon (6)884..1061
Gene:IDH1
Gene Synonym:
Exon (7)1062..1213
Gene:IDH1
Gene Synonym:
Exon (8)1214..1354
Gene:IDH1
Gene Synonym:
Exon (9)1355..1517
Gene:IDH1
Gene Synonym:
Exon (10)1518..2460
Gene:IDH1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001282387
Organism Homo sapiens (human)
Definition Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282387

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCCAAAA AAATCAGTGG CGGTTCTGTG GTAGAGATGC AAGGAGATGA AATGACACGA 
ATCATTTGGG AATTGATTAA AGAGAAACTC ATTTTTCCCT ACGTGGAATT GGATCTACAT
AGCTATGATT TAGGCATAGA GAATCGTGAT GCCACCAACG ACCAAGTCAC CAAGGATGCT
GCAGAAGCTA TAAAGAAGCA TAATGTTGGC GTCAAATGTG CCACTATCAC TCCTGATGAG
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCAAATGG CACCATACGA
AATATTCTGG GTGGCACGGT CTTCAGAGAA GCCATTATCT GCAAAAATAT CCCCCGGCTT
GTGAGTGGAT GGGTAAAACC TATCATCATA GGTCGTCATG CTTATGGGGA TCAATACAGA
GCAACTGATT TTGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAGTGAC
GGAACCCAAA AGGTGACATA CCTGGTACAT AACTTTGAAG AAGGTGGTGG TGTTGCCATG
GGGATGTATA ATCAAGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT
CTGTCTAAGG GTTGGCCTTT GTATCTGAGC ACCAAAAACA CTATTCTGAA GAAATATGAT
GGGCGTTTTA AAGACATCTT TCAGGAGATA TATGACAAGC AGTACAAGTC CCAGTTTGAA
GCTCAAAAGA TCTGGTATGA GCATAGGCTC ATCGACGACA TGGTGGCCCA AGCTATGAAA
TCAGAGGGAG GCTTCATCTG GGCCTGTAAA AACTATGATG GTGACGTGCA GTCGGACTCT
GTGGCCCAAG GGTATGGCTC TCTCGGCATG ATGACCAGCG TGCTGGTTTG TCCAGATGGC
AAGACAGTAG AAGCAGAGGC TGCCCACGGG ACTGTAACCC GTCACTACCG CATGTACCAG
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGAC CAGAGGGTTA
GCCCACAGAG CAAAGCTTGA TAACAATAAA GAGCTTGCCT TCTTTGCAAA TGCTTTGGAA
GAAGTCTCTA TTGAGACAAT TGAGGCTGGC TTCATGACCA AGGACTTGGC TGCTTGCATT
AAAGGTTTAC CCAATGTGCA ACGTTCTGAC TACTTGAATA CATTTGAGTT CATGGATAAA
CTTGGAGAAA ACTTGAAGAT CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21156
Clone ID Related Accession (Same CDS sequence) NM_001282386 , NM_001282387 , NM_005896
Accession Version NM_001282386.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1245bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product isocitrate dehydrogenase [NADP] cytoplasmic
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA707595.1, BC021046.1 and N21575.1. This sequence is a reference standard in the RefSeqGene project. On Sep 5, 2013 this sequence version replaced gi:530370284. Transcript Variant: This variant (2) has an alternate 5' UTR exon, compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCCAAAA AAATCAGTGG CGGTTCTGTG GTAGAGATGC AAGGAGATGA AATGACACGA 
ATCATTTGGG AATTGATTAA AGAGAAACTC ATTTTTCCCT ACGTGGAATT GGATCTACAT
AGCTATGATT TAGGCATAGA GAATCGTGAT GCCACCAACG ACCAAGTCAC CAAGGATGCT
GCAGAAGCTA TAAAGAAGCA TAATGTTGGC GTCAAATGTG CCACTATCAC TCCTGATGAG
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCAAATGG CACCATACGA
AATATTCTGG GTGGCACGGT CTTCAGAGAA GCCATTATCT GCAAAAATAT CCCCCGGCTT
GTGAGTGGAT GGGTAAAACC TATCATCATA GGTCGTCATG CTTATGGGGA TCAATACAGA
GCAACTGATT TTGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAGTGAC
GGAACCCAAA AGGTGACATA CCTGGTACAT AACTTTGAAG AAGGTGGTGG TGTTGCCATG
GGGATGTATA ATCAAGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT
CTGTCTAAGG GTTGGCCTTT GTATCTGAGC ACCAAAAACA CTATTCTGAA GAAATATGAT
GGGCGTTTTA AAGACATCTT TCAGGAGATA TATGACAAGC AGTACAAGTC CCAGTTTGAA
GCTCAAAAGA TCTGGTATGA GCATAGGCTC ATCGACGACA TGGTGGCCCA AGCTATGAAA
TCAGAGGGAG GCTTCATCTG GGCCTGTAAA AACTATGATG GTGACGTGCA GTCGGACTCT
GTGGCCCAAG GGTATGGCTC TCTCGGCATG ATGACCAGCG TGCTGGTTTG TCCAGATGGC
AAGACAGTAG AAGCAGAGGC TGCCCACGGG ACTGTAACCC GTCACTACCG CATGTACCAG
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGAC CAGAGGGTTA
GCCCACAGAG CAAAGCTTGA TAACAATAAA GAGCTTGCCT TCTTTGCAAA TGCTTTGGAA
GAAGTCTCTA TTGAGACAAT TGAGGCTGGC TTCATGACCA AGGACTTGGC TGCTTGCATT
AAAGGTTTAC CCAATGTGCA ACGTTCTGAC TACTTGAATA CATTTGAGTT CATGGATAAA
CTTGGAGAAA ACTTGAAGAT CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269315.1
CDS207..1451
Misc Feature(1)168..170(+)
Misc Feature(2)207..1439(+)
Misc Feature(3)210..212(+)
Misc Feature(4)486..506(+)
Misc Feature(5)621..623(+)
Misc Feature(6)840..842(+)
Misc Feature(7)1167..1169(+)
Exon (1)1..116
Gene:IDH1
Gene Synonym:
Exon (2)117..190
Gene:IDH1
Gene Synonym:
Exon (3)191..328
Gene:IDH1
Gene Synonym:
Exon (4)329..620
Gene:IDH1
Gene Synonym:
Exon (5)621..726
Gene:IDH1
Gene Synonym:
Exon (6)727..904
Gene:IDH1
Gene Synonym:
Exon (7)905..1056
Gene:IDH1
Gene Synonym:
Exon (8)1057..1197
Gene:IDH1
Gene Synonym:
Exon (9)1198..1360
Gene:IDH1
Gene Synonym:
Exon (10)1361..2303
Gene:IDH1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001282386
Organism Homo sapiens (human)
Definition Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282386

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGTCCAAAA AAATCAGTGG CGGTTCTGTG GTAGAGATGC AAGGAGATGA AATGACACGA 
ATCATTTGGG AATTGATTAA AGAGAAACTC ATTTTTCCCT ACGTGGAATT GGATCTACAT
AGCTATGATT TAGGCATAGA GAATCGTGAT GCCACCAACG ACCAAGTCAC CAAGGATGCT
GCAGAAGCTA TAAAGAAGCA TAATGTTGGC GTCAAATGTG CCACTATCAC TCCTGATGAG
AAGAGGGTTG AGGAGTTCAA GTTGAAACAA ATGTGGAAAT CACCAAATGG CACCATACGA
AATATTCTGG GTGGCACGGT CTTCAGAGAA GCCATTATCT GCAAAAATAT CCCCCGGCTT
GTGAGTGGAT GGGTAAAACC TATCATCATA GGTCGTCATG CTTATGGGGA TCAATACAGA
GCAACTGATT TTGTTGTTCC TGGGCCTGGA AAAGTAGAGA TAACCTACAC ACCAAGTGAC
GGAACCCAAA AGGTGACATA CCTGGTACAT AACTTTGAAG AAGGTGGTGG TGTTGCCATG
GGGATGTATA ATCAAGATAA GTCAATTGAA GATTTTGCAC ACAGTTCCTT CCAAATGGCT
CTGTCTAAGG GTTGGCCTTT GTATCTGAGC ACCAAAAACA CTATTCTGAA GAAATATGAT
GGGCGTTTTA AAGACATCTT TCAGGAGATA TATGACAAGC AGTACAAGTC CCAGTTTGAA
GCTCAAAAGA TCTGGTATGA GCATAGGCTC ATCGACGACA TGGTGGCCCA AGCTATGAAA
TCAGAGGGAG GCTTCATCTG GGCCTGTAAA AACTATGATG GTGACGTGCA GTCGGACTCT
GTGGCCCAAG GGTATGGCTC TCTCGGCATG ATGACCAGCG TGCTGGTTTG TCCAGATGGC
AAGACAGTAG AAGCAGAGGC TGCCCACGGG ACTGTAACCC GTCACTACCG CATGTACCAG
AAAGGACAGG AGACGTCCAC CAATCCCATT GCTTCCATTT TTGCCTGGAC CAGAGGGTTA
GCCCACAGAG CAAAGCTTGA TAACAATAAA GAGCTTGCCT TCTTTGCAAA TGCTTTGGAA
GAAGTCTCTA TTGAGACAAT TGAGGCTGGC TTCATGACCA AGGACTTGGC TGCTTGCATT
AAAGGTTTAC CCAATGTGCA ACGTTCTGAC TACTTGAATA CATTTGAGTT CATGGATAAA
CTTGGAGAAA ACTTGAAGAT CAAACTAGCT CAGGCCAAAC TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Up-regulated isocitrate dehydrogenase 1 suppresses proliferation, migration and invasion in osteosarcoma: in vitro and in vivo
Cancer Lett. 346 (1), 114-121 (2014)
Hu X, Liu Y, Qin C, Pan Z, Luo J, Yu A and Cheng Z.


book

Correlation of IDH1/2 mutation with clinicopathologic factors and prognosis in anaplastic gliomas: a report of 203 patients from China
J. Cancer Res. Clin. Oncol. 140 (1), 45-51 (2014)
Zhang CB, Bao ZS, Wang HJ, Yan W, Liu YW, Li MY, Zhang W, Chen L and Jiang T.


book

Prognostic or predictive value of MGMT promoter methylation in gliomas depends on IDH1 mutation
Neurology 81 (17), 1515-1522 (2013)
Wick W, Meisner C, Hentschel B, Platten M, Schilling A, Wiestler B, Sabel MC, Koeppen S, Ketter R, Weiler M, Tabatabai G, von Deimling A, Gramatzki D, Westphal M, Schackert G, Loeffler M, Simon M, Reifenberger G and Weller M.


book

[Mutation of isocitrate dehydrogenase gene in Chinese patients with glioma]
Zhonghua Bing Li Xue Za Zhi 42 (5), 292-298 (2013)
Pan Y, Qi XL, Wang LM, Dong RF, Zhang M, Zheng DF, Chang Q and Zhong YF.


book

Non-invasive in vivo assessment of IDH1 mutational status in glioma
Nat Commun 4, 2429 (2013)
Chaumeil MM, Larson PE, Yoshihara HA, Danforth OM, Vigneron DB, Nelson SJ, Pieper RO, Phillips JJ and Ronen SM.


book

Cytosolic isocitrate dehydrogenase in humans, mice, and voles and phylogenetic analysis of the enzyme family
Mol. Biol. Evol. 15 (12), 1674-1684 (1998)
Nekrutenko A, Hillis DM, Patton JC, Bradley RD and Baker RJ.


book

Fatty acid synthesis from glutamate in the adipose tissue of normal subjects and obese patients: an enzyme study
Biochem. Mol. Med. 54 (1), 19-25 (1995)
Belfiore F and Iannello S.


book

Probable assignment of soluble isocitrate dehydrogenase (IDH1) to 2q33.3
Hum. Genet. 71 (1), 37-40 (1985)
Narahara,K., Kimura,S., Kikkawa,K., Takahashi,Y., Wakita,Y., Kasai,R., Nagai,S., Nishibayashi,Y. and Kimoto,H.


book

Chromosome assignments of genes in man using mouse-human somatic cell hybrids: Cytoplasmic isocitrate dehydrogenase (IDH 1) and malate dehydrogenase (MDH 1) to chromosomes 2
Am. J. Hum. Genet. 26 (5), 604-613 (1974)
Creagan,R.P., Carritt,B., Chen,S., Kucherlapati,R., McMorris,F.A., Ricciuti,F., Tan,Y.H., Tischfield,J.A. and Ruddle,F.H.


book

Intracellular location and genetic control of isozymes of NADP-dependent isocitrate dehydrogenase and malate dehydrogenase
Ann. N. Y. Acad. Sci. 151 (1), 429-440 (1968)
Henderson,N.S.


 
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