×

APOE cDNA ORF clone, Homo sapiens(Human)

Gene Symbol APOE
Entrez Gene ID 348
Full Name apolipoprotein E
Synonyms AD2,APO-E,ApoE4,LDLCQ5,LPG
General protein information
Preferred Names

apolipoprotein E

Names

apolipoprotein E
apolipoprotein E3

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

19

19q13.32

Summary The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jun 2016].
Disorder MIM:

107741

Disorder Html: Hyperlipoproteinemia, type III (3); {Myocardial infarction

mRNA and Protein(s)

mRNA Protein Name
NM_001302690.1 NP_001289619.1 apolipoprotein E isoform b precursor
NM_001302689.1 NP_001289618.1 apolipoprotein E isoform b precursor
NM_001302691.1 NP_001289620.1 apolipoprotein E isoform b precursor
NM_001302688.1 NP_001289617.1 apolipoprotein E isoform a precursor
NM_000041.3 NP_000032.1 apolipoprotein E isoform b precursor



Pan troglodytes (chimpanzee) APOE NP_001009007.1
Macaca mulatta (rhesus monkey) APOE XP_001104482.2
Mus musculus (house mouse) Apoe NP_033826.2
Canis lupus familiaris (dog) APOE XP_866043.1
Bos taurus (cattle) APOE NP_776416.1
Rattus norvegicus (Norway rat) Apoe NP_620183.2
Xenopus tropicalis (tropical clawed frog) apoe XP_004912063.1
Homo sapiens (human) APOE NP_000032.1


Related articles in PubMed

Frequency Specific Effects of
Liang Y, Li Z, Wei J, Li C, Zhang X, Neuroimaging Initiative AD
BioMed research international20179823501(2017)

Integrated late onset Alzheimer's disease (LOAD) susceptibility genes: Cholesterol metabolism and trafficking perspectives.
Dong HK, Gim JA, Yeo SH, Kim HS
Gene59710-16(2017 Jan)

Association of apolipoprotein E polymorphism with plasma lipid disorders, independent of obesity-related traits in Vietnamese children.
Hanh NT, Nhung BT, Dao DT, Tuyet LT, Hop LT, Binh TQ, Thuc VT
Lipids in health and disease15(1)176(2016 Oct)

Apolipoprotein E Gene Variants and Risk of Coronary Heart Disease: A Meta-Analysis.
Xu M, Zhao J, Zhang Y, Ma X, Dai Q, Zhi H, Wang B, Wang L
BioMed research international20163912175(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ApoE polymorphism is related to coronary disease in the Chinese Han population, with smoking and alcohol drinking increasing the genetic predisposition to disease.
Title: Combined effects of apoE-CI-CII cluster and LDL-R gene polymorphisms on chromosome 19 and coronary artery disease risk.

Observational study of gene-disease association. (HuGE Navigator)
Title: Apolipoprotein (APOE) gene is associated with progression of age-related macular degeneration (AMD).

In multiple sclerosis APOE epsilon4 polymorphism is not associated with a more severe clinical course and does not appear to influence recovery of exacerbations.
Title: No association of apolipoprotein E epsilon4 genotype with faster progression or less recovery of relapses in a Spanish cohort of multiple sclerosis.

Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Apolipoprotein E polymorphism and preclinical carotid artery disease in untreated hypertensive men.

Observational study of gene-disease association. (HuGE Navigator)
Title: Lack of association of lobar intracerebral hemorrhage with apolipoprotein E genotype in an unselected population.

The following APOE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APOE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu27296 NM_001302689.1
Latest version!
Homo sapiens apolipoprotein E (APOE), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu27296 NM_000041.3
Latest version!
Homo sapiens apolipoprotein E (APOE), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu27296 NM_001302690.1
Latest version!
Homo sapiens apolipoprotein E (APOE), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu27296 NM_001302691.1
Latest version!
Homo sapiens apolipoprotein E (APOE), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu52233 NM_001302688.1
Latest version!
Homo sapiens apolipoprotein E (APOE), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu27296
Clone ID Related Accession (Same CDS sequence) NM_001302690.1 , NM_001302689.1 , NM_001302691.1 , NM_000041.3
Accession Version NM_001302690.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product apolipoprotein E isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CA771535.1, HY074888.1, BC003557.1 and AL037046.3. Transcript Variant: This variant (4) contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Variants 2, 3, 4 and 5 all encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY074888.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289619.1
CDS148..1101
Misc Feature(1)79..81
Misc Feature(2)385..912
Misc Feature(3)403..945
Misc Feature(4)574..576
Misc Feature(5)586..588
Misc Feature(6)619..651
Misc Feature(7)631..642
Misc Feature(8)832..855
Exon (1)1..124
Exon (2)125..190
Exon (3)191..383
Exon (4)384..1246
Translation

Target ORF information:

RefSeq Version NM_001302690.1
Organism Homo sapiens(Human)
Definition Homo sapiens apolipoprotein E (APOE), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001302690.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27296
Clone ID Related Accession (Same CDS sequence) NM_001302690.1 , NM_001302689.1 , NM_001302691.1 , NM_000041.3
Accession Version NM_001302689.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product apolipoprotein E isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB486513.1, HY074384.1, BC003557.1 and AL037046.3. Transcript Variant: This variant (3) contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Variants 2, 3, 4 and 5 all encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB486513.1, HY075493.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289618.1
CDS48..1001
Misc Feature(1)285..812
Misc Feature(2)303..845
Misc Feature(3)474..476
Misc Feature(4)486..488
Misc Feature(5)519..551
Misc Feature(6)531..542
Misc Feature(7)732..755
Exon (1)1..24
Exon (2)25..90
Exon (3)91..283
Exon (4)284..1146
Translation

Target ORF information:

RefSeq Version NM_001302689.1
Organism Homo sapiens(Human)
Definition Homo sapiens apolipoprotein E (APOE), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001302689.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27296
Clone ID Related Accession (Same CDS sequence) NM_001302690.1 , NM_001302689.1 , NM_001302691.1 , NM_000041.3
Accession Version NM_001302691.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product apolipoprotein E isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY056112.1, BI550631.1, BC003557.1 and AL037046.3. Transcript Variant: This variant (5) contains an alternate 5' terminal exon and uses an alternate splice site in another 5' exon, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Variants 2, 3, 4 and 5 all encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BI550631.1, BI670331.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289620.1
CDS132..1085
Misc Feature(1)42..44
Misc Feature(2)369..896
Misc Feature(3)387..929
Misc Feature(4)558..560
Misc Feature(5)570..572
Misc Feature(6)603..635
Misc Feature(7)615..626
Misc Feature(8)816..839
Exon (1)1..93
Exon (2)94..174
Exon (3)175..367
Exon (4)368..1230
Translation

Target ORF information:

RefSeq Version NM_001302691.1
Organism Homo sapiens(Human)
Definition Homo sapiens apolipoprotein E (APOE), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001302691.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27296
Clone ID Related Accession (Same CDS sequence) NM_001302690.1 , NM_001302689.1 , NM_001302691.1 , NM_000041.3
Accession Version NM_000041.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product apolipoprotein E isoform b precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY056112.1, BC003557.1 and AL037046.3. This sequence is a reference standard in the RefSeqGene project. On Nov 7, 2014 this sequence version replaced NM_000041.2. Transcript Variant: This variant (2) contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Variants 2, 3, 4 and 5 all encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279835.8182.1, ERR279859.3064.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000032.1
CDS117..1070
Misc Feature(1)42..44
Misc Feature(2)354..881
Misc Feature(3)372..914
Misc Feature(4)393..395
Misc Feature(5)543..545
Misc Feature(6)555..557
Misc Feature(7)588..620
Misc Feature(8)600..611
Misc Feature(9)750..752
Misc Feature(10)801..824
Misc Feature(11)972..974
Misc Feature(12)984..986
Exon (1)1..93
Exon (2)94..159
Exon (3)160..352
Exon (4)353..1215
Translation

Target ORF information:

RefSeq Version NM_000041.3
Organism Homo sapiens(Human)
Definition Homo sapiens apolipoprotein E (APOE), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000041.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 
GAGCAAGCGG TGGAGACAGA GCCGGAGCCC GAGCTGCGCC AGCAGACCGA GTGGCAGAGC
GGCCAGCGCT GGGAACTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA
CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCTCCCAGG TCACCCAGGA ACTGAGGGCG
CTGATGGACG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAACTG
ACCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC
CGGCTGGGCG CGGACATGGA GGACGTGTGC GGCCGCCTGG TGCAGTACCG CGGCGAGGTG
CAGGCCATGC TCGGCCAGAG CACCGAGGAG CTGCGGGTGC GCCTCGCCTC CCACCTGCGC
AAGCTGCGTA AGCGGCTCCT CCGCGATGCC GATGACCTGC AGAAGCGCCT GGCAGTGTAC
CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGCCTCAGCG CCATCCGCGA GCGCCTGGGG
CCCCTGGTGG AACAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CGGCCAGCCG
CTACAGGAGC GGGCCCAGGC CTGGGGCGAG CGGCTGCGCG CGCGGATGGA GGAGATGGGC
AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG
CTGGAGGAGC AGGCCCAGCA GATACGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG
AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCCGGGCT GGTGGAGAAG
GTGCAGGCTG CCGTGGGCAC CAGCGCCGCC CCTGTGCCCA GCGACAATCA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu52233
Clone ID Related Accession (Same CDS sequence) NM_001302688.1
Accession Version NM_001302688.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1032bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-06
Organism Homo sapiens(Human)
Product apolipoprotein E isoform a precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY056112.1, BM042032.1, BC003557.1 and AL037046.3. On Nov 7, 2014 this sequence version replaced XM_005258867.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279841.4394.1, ERR279831.1485.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCTCAG GGGCCTCTAG AAAGAGCTGG GACCCTGGGA ACCCCTGGCC TCCAGACTGG 
CCAATCACAG GCAGGAAGAT GAAGGTTCTG TGGGCTGCGT TGCTGGTCAC ATTCCTGGCA
GGATGCCAGG CCAAGGTGGA GCAAGCGGTG GAGACAGAGC CGGAGCCCGA GCTGCGCCAG
CAGACCGAGT GGCAGAGCGG CCAGCGCTGG GAACTGGCAC TGGGTCGCTT TTGGGATTAC
CTGCGCTGGG TGCAGACACT GTCTGAGCAG GTGCAGGAGG AGCTGCTCAG CTCCCAGGTC
ACCCAGGAAC TGAGGGCGCT GATGGACGAG ACCATGAAGG AGTTGAAGGC CTACAAATCG
GAACTGGAGG AACAACTGAC CCCGGTGGCG GAGGAGACGC GGGCACGGCT GTCCAAGGAG
CTGCAGGCGG CGCAGGCCCG GCTGGGCGCG GACATGGAGG ACGTGTGCGG CCGCCTGGTG
CAGTACCGCG GCGAGGTGCA GGCCATGCTC GGCCAGAGCA CCGAGGAGCT GCGGGTGCGC
CTCGCCTCCC ACCTGCGCAA GCTGCGTAAG CGGCTCCTCC GCGATGCCGA TGACCTGCAG
AAGCGCCTGG CAGTGTACCA GGCCGGGGCC CGCGAGGGCG CCGAGCGCGG CCTCAGCGCC
ATCCGCGAGC GCCTGGGGCC CCTGGTGGAA CAGGGCCGCG TGCGGGCCGC CACTGTGGGC
TCCCTGGCCG GCCAGCCGCT ACAGGAGCGG GCCCAGGCCT GGGGCGAGCG GCTGCGCGCG
CGGATGGAGG AGATGGGCAG CCGGACCCGC GACCGCCTGG ACGAGGTGAA GGAGCAGGTG
GCGGAGGTGC GCGCCAAGCT GGAGGAGCAG GCCCAGCAGA TACGCCTGCA GGCCGAGGCC
TTCCAGGCCC GCCTCAAGAG CTGGTTCGAG CCCCTGGTGG AAGACATGCA GCGCCAGTGG
GCCGGGCTGG TGGAGAAGGT GCAGGCTGCC GTGGGCACCA GCGCCGCCCC TGTGCCCAGC
GACAATCACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001289617.1
CDS121..1152
Misc Feature(1)19..21
Misc Feature(2)454..996
Exon (1)1..175
Exon (2)176..241
Exon (3)242..434
Exon (4)435..1297
Translation

Target ORF information:

RefSeq Version NM_001302688.1
Organism Homo sapiens(Human)
Definition Homo sapiens apolipoprotein E (APOE), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001302688.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGAGCTCAG GGGCCTCTAG AAAGAGCTGG GACCCTGGGA ACCCCTGGCC TCCAGACTGG 
CCAATCACAG GCAGGAAGAT GAAGGTTCTG TGGGCTGCGT TGCTGGTCAC ATTCCTGGCA
GGATGCCAGG CCAAGGTGGA GCAAGCGGTG GAGACAGAGC CGGAGCCCGA GCTGCGCCAG
CAGACCGAGT GGCAGAGCGG CCAGCGCTGG GAACTGGCAC TGGGTCGCTT TTGGGATTAC
CTGCGCTGGG TGCAGACACT GTCTGAGCAG GTGCAGGAGG AGCTGCTCAG CTCCCAGGTC
ACCCAGGAAC TGAGGGCGCT GATGGACGAG ACCATGAAGG AGTTGAAGGC CTACAAATCG
GAACTGGAGG AACAACTGAC CCCGGTGGCG GAGGAGACGC GGGCACGGCT GTCCAAGGAG
CTGCAGGCGG CGCAGGCCCG GCTGGGCGCG GACATGGAGG ACGTGTGCGG CCGCCTGGTG
CAGTACCGCG GCGAGGTGCA GGCCATGCTC GGCCAGAGCA CCGAGGAGCT GCGGGTGCGC
CTCGCCTCCC ACCTGCGCAA GCTGCGTAAG CGGCTCCTCC GCGATGCCGA TGACCTGCAG
AAGCGCCTGG CAGTGTACCA GGCCGGGGCC CGCGAGGGCG CCGAGCGCGG CCTCAGCGCC
ATCCGCGAGC GCCTGGGGCC CCTGGTGGAA CAGGGCCGCG TGCGGGCCGC CACTGTGGGC
TCCCTGGCCG GCCAGCCGCT ACAGGAGCGG GCCCAGGCCT GGGGCGAGCG GCTGCGCGCG
CGGATGGAGG AGATGGGCAG CCGGACCCGC GACCGCCTGG ACGAGGTGAA GGAGCAGGTG
GCGGAGGTGC GCGCCAAGCT GGAGGAGCAG GCCCAGCAGA TACGCCTGCA GGCCGAGGCC
TTCCAGGCCC GCCTCAAGAG CTGGTTCGAG CCCCTGGTGG AAGACATGCA GCGCCAGTGG
GCCGGGCTGG TGGAGAAGGT GCAGGCTGCC GTGGGCACCA GCGCCGCCCC TGTGCCCAGC
GACAATCACT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Frequency Specific Effects of
BioMed research international20179823501(2017)
Liang Y,Li Z,Wei J,Li C,Zhang X,Neuroimaging Initiative AD


book

Integrated late onset Alzheimer's disease (LOAD) susceptibility genes: Cholesterol metabolism and trafficking perspectives.
Gene59710-16(2017 Jan)
Dong HK,Gim JA,Yeo SH,Kim HS


book

Association of apolipoprotein E polymorphism with plasma lipid disorders, independent of obesity-related traits in Vietnamese children.
Lipids in health and disease15(1)176(2016 Oct)
Hanh NT,Nhung BT,Dao DT,Tuyet LT,Hop LT,Binh TQ,Thuc VT


book

Apolipoprotein E Gene Variants and Risk of Coronary Heart Disease: A Meta-Analysis.
BioMed research international20163912175(2016)
Xu M,Zhao J,Zhang Y,Ma X,Dai Q,Zhi H,Wang B,Wang L


 
*
*
*
*