KARS1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following KARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu05050 NM_005548.2
Latest version!
Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu18770 NM_001130089.1
Latest version!
Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu86026 XM_017023217.1
Latest version!
Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu05050
    Clone ID Related Accession (Same CDS sequence) NM_005548.2
    Accession Version NM_005548.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1794bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product lysine--tRNA ligase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315687.1, AF285758.1 and AA503390.1. On Jul 16, 2008 this sequence version replaced NM_005548.1. Transcript Variant: This variant (2) lacks an internal exon in the 5' region, which results in an upstream AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.429235.1, SRR1803612.179789.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCCG TGCAGGCGGC CGAGGTGAAA GTGGATGGCA GCGAGCCGAA ACTGAGCAAG 
    AATGAGCTGA AGAGACGCCT GAAAGCTGAG AAGAAAGTAG CAGAGAAGGA GGCCAAACAG
    AAAGAGCTCA GTGAGAAACA GCTAAGCCAA GCCACTGCTG CTGCCACCAA CCACACCACT
    GATAATGGTG TGGGTCCTGA GGAAGAGAGC GTGGACCCAA ATCAATACTA CAAAATCCGC
    AGTCAAGCAA TTCATCAGCT GAAGGTCAAT GGGGAAGACC CATACCCACA CAAGTTCCAT
    GTAGACATCT CACTCACTGA CTTCATCCAA AAATATAGTC ACCTGCAGCC TGGGGATCAC
    CTGACTGACA TCACCTTAAA GGTGGCAGGT AGGATCCATG CCAAAAGAGC TTCTGGGGGA
    AAGCTCATCT TCTATGATCT TCGAGGAGAG GGGGTGAAGT TGCAAGTCAT GGCCAATTCC
    AGAAATTATA AATCAGAAGA AGAATTTATT CATATTAATA ACAAACTGCG TCGGGGAGAC
    ATAATTGGAG TTCAGGGGAA TCCTGGTAAA ACCAAGAAGG GTGAGCTGAG CATCATTCCG
    TATGAGATCA CACTGCTGTC TCCCTGTTTG CATATGTTAC CTCATCTTCA CTTTGGCCTC
    AAAGACAAGG AAACAAGGTA TCGCCAGAGA TACTTGGACT TGATCCTGAA TGACTTTGTG
    AGGCAGAAAT TTATCATCCG CTCTAAGATC ATCACATATA TAAGAAGTTT CTTAGATGAG
    CTGGGATTCC TAGAGATTGA AACTCCCATG ATGAACATCA TCCCAGGGGG AGCCGTGGCC
    AAGCCTTTCA TCACTTATCA CAACGAGCTG GACATGAACT TATATATGAG AATTGCTCCA
    GAACTCTATC ATAAGATGCT TGTGGTTGGT GGCATCGACC GGGTTTATGA AATTGGACGC
    CAGTTCCGGA ATGAGGGGAT TGATTTGACG CACAATCCTG AGTTCACCAC CTGTGAGTTC
    TACATGGCCT ATGCAGACTA TCACGATCTC ATGGAAATCA CGGAGAAGAT GGTTTCAGGG
    ATGGTGAAGC ATATTACAGG CAGTTACAAG GTCACCTACC ACCCAGATGG CCCAGAGGGC
    CAAGCCTACG ATGTTGACTT CACCCCACCC TTCCGGCGAA TCAACATGGT AGAAGAGCTT
    GAGAAAGCCC TGGGGATGAA GCTGCCAGAA ACGAACCTCT TTGAAACTGA AGAAACTCGC
    AAAATTCTTG ATGATATCTG TGTGGCAAAA GCTGTTGAAT GCCCTCCACC TCGGACCACA
    GCCAGGCTCC TTGACAAGCT TGTTGGGGAG TTCCTGGAAG TGACTTGCAT CAATCCTACA
    TTCATCTGTG ATCACCCACA GATAATGAGC CCTTTGGCTA AATGGCACCG CTCTAAAGAG
    GGTCTGACTG AGCGCTTTGA GCTGTTTGTC ATGAAGAAAG AGATATGCAA TGCGTATACT
    GAGCTGAATG ATCCCATGCG GCAGCGGCAG CTTTTTGAAG AACAGGCCAA GGCCAAGGCT
    GCAGGTGATG ATGAGGCCAT GTTCATAGAT GAAAACTTCT GTACTGCCCT GGAATATGGG
    CTGCCCCCCA CAGCTGGCTG GGGCATGGGC ATTGATCGAG TCGCCATGTT TCTCACGGAC
    TCCAACAACA TCAAGGAAGT ACTTCTGTTT CCTGCCATGA AACCCGAAGA CAAGAAGGAG
    AATGTAGCAA CCACTGATAC ACTGGAAAGC ACAACAGTTG GCACTTCTGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005539.1
    CDS49..1842
    Translation

    Target ORF information:

    RefSeq Version NM_005548.2
    Organism Homo sapiens(human)
    Definition Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005548.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGCCG TGCAGGCGGC CGAGGTGAAA GTGGATGGCA GCGAGCCGAA ACTGAGCAAG 
    AATGAGCTGA AGAGACGCCT GAAAGCTGAG AAGAAAGTAG CAGAGAAGGA GGCCAAACAG
    AAAGAGCTCA GTGAGAAACA GCTAAGCCAA GCCACTGCTG CTGCCACCAA CCACACCACT
    GATAATGGTG TGGGTCCTGA GGAAGAGAGC GTGGACCCAA ATCAATACTA CAAAATCCGC
    AGTCAAGCAA TTCATCAGCT GAAGGTCAAT GGGGAAGACC CATACCCACA CAAGTTCCAT
    GTAGACATCT CACTCACTGA CTTCATCCAA AAATATAGTC ACCTGCAGCC TGGGGATCAC
    CTGACTGACA TCACCTTAAA GGTGGCAGGT AGGATCCATG CCAAAAGAGC TTCTGGGGGA
    AAGCTCATCT TCTATGATCT TCGAGGAGAG GGGGTGAAGT TGCAAGTCAT GGCCAATTCC
    AGAAATTATA AATCAGAAGA AGAATTTATT CATATTAATA ACAAACTGCG TCGGGGAGAC
    ATAATTGGAG TTCAGGGGAA TCCTGGTAAA ACCAAGAAGG GTGAGCTGAG CATCATTCCG
    TATGAGATCA CACTGCTGTC TCCCTGTTTG CATATGTTAC CTCATCTTCA CTTTGGCCTC
    AAAGACAAGG AAACAAGGTA TCGCCAGAGA TACTTGGACT TGATCCTGAA TGACTTTGTG
    AGGCAGAAAT TTATCATCCG CTCTAAGATC ATCACATATA TAAGAAGTTT CTTAGATGAG
    CTGGGATTCC TAGAGATTGA AACTCCCATG ATGAACATCA TCCCAGGGGG AGCCGTGGCC
    AAGCCTTTCA TCACTTATCA CAACGAGCTG GACATGAACT TATATATGAG AATTGCTCCA
    GAACTCTATC ATAAGATGCT TGTGGTTGGT GGCATCGACC GGGTTTATGA AATTGGACGC
    CAGTTCCGGA ATGAGGGGAT TGATTTGACG CACAATCCTG AGTTCACCAC CTGTGAGTTC
    TACATGGCCT ATGCAGACTA TCACGATCTC ATGGAAATCA CGGAGAAGAT GGTTTCAGGG
    ATGGTGAAGC ATATTACAGG CAGTTACAAG GTCACCTACC ACCCAGATGG CCCAGAGGGC
    CAAGCCTACG ATGTTGACTT CACCCCACCC TTCCGGCGAA TCAACATGGT AGAAGAGCTT
    GAGAAAGCCC TGGGGATGAA GCTGCCAGAA ACGAACCTCT TTGAAACTGA AGAAACTCGC
    AAAATTCTTG ATGATATCTG TGTGGCAAAA GCTGTTGAAT GCCCTCCACC TCGGACCACA
    GCCAGGCTCC TTGACAAGCT TGTTGGGGAG TTCCTGGAAG TGACTTGCAT CAATCCTACA
    TTCATCTGTG ATCACCCACA GATAATGAGC CCTTTGGCTA AATGGCACCG CTCTAAAGAG
    GGTCTGACTG AGCGCTTTGA GCTGTTTGTC ATGAAGAAAG AGATATGCAA TGCGTATACT
    GAGCTGAATG ATCCCATGCG GCAGCGGCAG CTTTTTGAAG AACAGGCCAA GGCCAAGGCT
    GCAGGTGATG ATGAGGCCAT GTTCATAGAT GAAAACTTCT GTACTGCCCT GGAATATGGG
    CTGCCCCCCA CAGCTGGCTG GGGCATGGGC ATTGATCGAG TCGCCATGTT TCTCACGGAC
    TCCAACAACA TCAAGGAAGT ACTTCTGTTT CCTGCCATGA AACCCGAAGA CAAGAAGGAG
    AATGTAGCAA CCACTGATAC ACTGGAAAGC ACAACAGTTG GCACTTCTGT CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18770
    Clone ID Related Accession (Same CDS sequence) NM_001130089.1
    Accession Version NM_001130089.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product lysine--tRNA ligase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315687.1, AF285758.1, BM781594.1 and AA503390.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.126450.1, SRR1660809.13712.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTTGACGC AAGCTGCTGT AAGGCTTGTT AGGGGGTCCC TGCGCAAAAC CTCCTGGGCA 
    GAGTGGGGTC ACAGGGAACT GCGACTGGGT CAACTTGCTC CTTTCACAGC GCCTCACAAG
    GACAAGTCAT TTTCTGATCA AAGAAGTGAG CTGAAGAGAC GCCTGAAAGC TGAGAAGAAA
    GTAGCAGAGA AGGAGGCCAA ACAGAAAGAG CTCAGTGAGA AACAGCTAAG CCAAGCCACT
    GCTGCTGCCA CCAACCACAC CACTGATAAT GGTGTGGGTC CTGAGGAAGA GAGCGTGGAC
    CCAAATCAAT ACTACAAAAT CCGCAGTCAA GCAATTCATC AGCTGAAGGT CAATGGGGAA
    GACCCATACC CACACAAGTT CCATGTAGAC ATCTCACTCA CTGACTTCAT CCAAAAATAT
    AGTCACCTGC AGCCTGGGGA TCACCTGACT GACATCACCT TAAAGGTGGC AGGTAGGATC
    CATGCCAAAA GAGCTTCTGG GGGAAAGCTC ATCTTCTATG ATCTTCGAGG AGAGGGGGTG
    AAGTTGCAAG TCATGGCCAA TTCCAGAAAT TATAAATCAG AAGAAGAATT TATTCATATT
    AATAACAAAC TGCGTCGGGG AGACATAATT GGAGTTCAGG GGAATCCTGG TAAAACCAAG
    AAGGGTGAGC TGAGCATCAT TCCGTATGAG ATCACACTGC TGTCTCCCTG TTTGCATATG
    TTACCTCATC TTCACTTTGG CCTCAAAGAC AAGGAAACAA GGTATCGCCA GAGATACTTG
    GACTTGATCC TGAATGACTT TGTGAGGCAG AAATTTATCA TCCGCTCTAA GATCATCACA
    TATATAAGAA GTTTCTTAGA TGAGCTGGGA TTCCTAGAGA TTGAAACTCC CATGATGAAC
    ATCATCCCAG GGGGAGCCGT GGCCAAGCCT TTCATCACTT ATCACAACGA GCTGGACATG
    AACTTATATA TGAGAATTGC TCCAGAACTC TATCATAAGA TGCTTGTGGT TGGTGGCATC
    GACCGGGTTT ATGAAATTGG ACGCCAGTTC CGGAATGAGG GGATTGATTT GACGCACAAT
    CCTGAGTTCA CCACCTGTGA GTTCTACATG GCCTATGCAG ACTATCACGA TCTCATGGAA
    ATCACGGAGA AGATGGTTTC AGGGATGGTG AAGCATATTA CAGGCAGTTA CAAGGTCACC
    TACCACCCAG ATGGCCCAGA GGGCCAAGCC TACGATGTTG ACTTCACCCC ACCCTTCCGG
    CGAATCAACA TGGTAGAAGA GCTTGAGAAA GCCCTGGGGA TGAAGCTGCC AGAAACGAAC
    CTCTTTGAAA CTGAAGAAAC TCGCAAAATT CTTGATGATA TCTGTGTGGC AAAAGCTGTT
    GAATGCCCTC CACCTCGGAC CACAGCCAGG CTCCTTGACA AGCTTGTTGG GGAGTTCCTG
    GAAGTGACTT GCATCAATCC TACATTCATC TGTGATCACC CACAGATAAT GAGCCCTTTG
    GCTAAATGGC ACCGCTCTAA AGAGGGTCTG ACTGAGCGCT TTGAGCTGTT TGTCATGAAG
    AAAGAGATAT GCAATGCGTA TACTGAGCTG AATGATCCCA TGCGGCAGCG GCAGCTTTTT
    GAAGAACAGG CCAAGGCCAA GGCTGCAGGT GATGATGAGG CCATGTTCAT AGATGAAAAC
    TTCTGTACTG CCCTGGAATA TGGGCTGCCC CCCACAGCTG GCTGGGGCAT GGGCATTGAT
    CGAGTCGCCA TGTTTCTCAC GGACTCCAAC AACATCAAGG AAGTACTTCT GTTTCCTGCC
    ATGAAACCCG AAGACAAGAA GGAGAATGTA GCAACCACTG ATACACTGGA AAGCACAACA
    GTTGGCACTT CTGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001123561.1
    CDS145..2022
    Translation

    Target ORF information:

    RefSeq Version NM_001130089.1
    Organism Homo sapiens(human)
    Definition Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001130089.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTTGACGC AAGCTGCTGT AAGGCTTGTT AGGGGGTCCC TGCGCAAAAC CTCCTGGGCA 
    GAGTGGGGTC ACAGGGAACT GCGACTGGGT CAACTTGCTC CTTTCACAGC GCCTCACAAG
    GACAAGTCAT TTTCTGATCA AAGAAGTGAG CTGAAGAGAC GCCTGAAAGC TGAGAAGAAA
    GTAGCAGAGA AGGAGGCCAA ACAGAAAGAG CTCAGTGAGA AACAGCTAAG CCAAGCCACT
    GCTGCTGCCA CCAACCACAC CACTGATAAT GGTGTGGGTC CTGAGGAAGA GAGCGTGGAC
    CCAAATCAAT ACTACAAAAT CCGCAGTCAA GCAATTCATC AGCTGAAGGT CAATGGGGAA
    GACCCATACC CACACAAGTT CCATGTAGAC ATCTCACTCA CTGACTTCAT CCAAAAATAT
    AGTCACCTGC AGCCTGGGGA TCACCTGACT GACATCACCT TAAAGGTGGC AGGTAGGATC
    CATGCCAAAA GAGCTTCTGG GGGAAAGCTC ATCTTCTATG ATCTTCGAGG AGAGGGGGTG
    AAGTTGCAAG TCATGGCCAA TTCCAGAAAT TATAAATCAG AAGAAGAATT TATTCATATT
    AATAACAAAC TGCGTCGGGG AGACATAATT GGAGTTCAGG GGAATCCTGG TAAAACCAAG
    AAGGGTGAGC TGAGCATCAT TCCGTATGAG ATCACACTGC TGTCTCCCTG TTTGCATATG
    TTACCTCATC TTCACTTTGG CCTCAAAGAC AAGGAAACAA GGTATCGCCA GAGATACTTG
    GACTTGATCC TGAATGACTT TGTGAGGCAG AAATTTATCA TCCGCTCTAA GATCATCACA
    TATATAAGAA GTTTCTTAGA TGAGCTGGGA TTCCTAGAGA TTGAAACTCC CATGATGAAC
    ATCATCCCAG GGGGAGCCGT GGCCAAGCCT TTCATCACTT ATCACAACGA GCTGGACATG
    AACTTATATA TGAGAATTGC TCCAGAACTC TATCATAAGA TGCTTGTGGT TGGTGGCATC
    GACCGGGTTT ATGAAATTGG ACGCCAGTTC CGGAATGAGG GGATTGATTT GACGCACAAT
    CCTGAGTTCA CCACCTGTGA GTTCTACATG GCCTATGCAG ACTATCACGA TCTCATGGAA
    ATCACGGAGA AGATGGTTTC AGGGATGGTG AAGCATATTA CAGGCAGTTA CAAGGTCACC
    TACCACCCAG ATGGCCCAGA GGGCCAAGCC TACGATGTTG ACTTCACCCC ACCCTTCCGG
    CGAATCAACA TGGTAGAAGA GCTTGAGAAA GCCCTGGGGA TGAAGCTGCC AGAAACGAAC
    CTCTTTGAAA CTGAAGAAAC TCGCAAAATT CTTGATGATA TCTGTGTGGC AAAAGCTGTT
    GAATGCCCTC CACCTCGGAC CACAGCCAGG CTCCTTGACA AGCTTGTTGG GGAGTTCCTG
    GAAGTGACTT GCATCAATCC TACATTCATC TGTGATCACC CACAGATAAT GAGCCCTTTG
    GCTAAATGGC ACCGCTCTAA AGAGGGTCTG ACTGAGCGCT TTGAGCTGTT TGTCATGAAG
    AAAGAGATAT GCAATGCGTA TACTGAGCTG AATGATCCCA TGCGGCAGCG GCAGCTTTTT
    GAAGAACAGG CCAAGGCCAA GGCTGCAGGT GATGATGAGG CCATGTTCAT AGATGAAAAC
    TTCTGTACTG CCCTGGAATA TGGGCTGCCC CCCACAGCTG GCTGGGGCAT GGGCATTGAT
    CGAGTCGCCA TGTTTCTCAC GGACTCCAAC AACATCAAGG AAGTACTTCT GTTTCCTGCC
    ATGAAACCCG AAGACAAGAA GGAGAATGTA GCAACCACTG ATACACTGGA AAGCACAACA
    GTTGGCACTT CTGTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu86026
    Clone ID Related Accession (Same CDS sequence) XM_017023217.1
    Accession Version XM_017023217.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product lysine--tRNA ligase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCCAATT CCAGAAATTA TAAATCAGAA GAAGAATTTA TTCATATTAA TAACAAACTG 
    CGTCGGGGAG ACATAATTGG AGTTCAGGGG AATCCTGGTA AAACCAAGAA GGGTGAGCTG
    AGCATCATTC CGTATGAGAT CACACTGCTG TCTCCCTGTT TGCATATGTT ACCTCATCTT
    CACTTTGGCC TCAAAGACAA GGAAACAAGG TATCGCCAGA GATACTTGGA CTTGATCCTG
    AATGACTTTG TGAGGCAGAA ATTTATCATC CGCTCTAAGA TCATCACATA TATAAGAAGT
    TTCTTAGATG AGCTGGGATT CCTAGAGATT GAAACTCCCA TGATGAACAT CATCCCAGGG
    GGAGCCGTGG CCAAGCCTTT CATCACTTAT CACAACGAGC TGGACATGAA CTTATATATG
    AGAATTGCTC CAGAACTCTA TCATAAGATG CTTGTGGTTG GTGGCATCGA CCGGGTTTAT
    GAAATTGGAC GCCAGTTCCG GAATGAGGGG ATTGATTTGA CGCACAATCC TGAGTTCACC
    ACCTGTGAGT TCTACATGGC CTATGCAGAC TATCACGATC TCATGGAAAT CACGGAGAAG
    ATGGTTTCAG GGATGGTGAA GCATATTACA GGCAGTTACA AGGTCACCTA CCACCCAGAT
    GGCCCAGAGG GCCAAGCCTA CGATGTTGAC TTCACCCCAC CCTTCCGGCG AATCAACATG
    GTAGAAGAGC TTGAGAAAGC CCTGGGGATG AAGCTGCCAG AAACGAACCT CTTTGAAACT
    GAAGAAACTC GCAAAATTCT TGATGATATC TGTGTGGCAA AAGCTGTTGA ATGCCCTCCA
    CCTCGGACCA CAGCCAGGCT CCTTGACAAG CTTGTTGGGG AGTTCCTGGA AGTGACTTGC
    ATCAATCCTA CATTCATCTG TGATCACCCA CAGATAATGA GCCCTTTGGC TAAATGGCAC
    CGCTCTAAAG AGGGTCTGAC TGAGCGCTTT GAGCTGTTTG TCATGAAGAA AGAGATATGC
    AATGCGTATA CTGAGCTGAA TGATCCCATG CGGCAGCGGC AGCTTTTTGA AGAACAGGCC
    AAGGCCAAGG CTGCAGGTGA TGATGAGGCC ATGTTCATAG ATGAAAACTT CTGTACTGCC
    CTGGAATATG GGCTGCCCCC CACAGCTGGC TGGGGCATGG GCATTGATCG AGTCGCCATG
    TTTCTCACGG ACTCCAACAA CATCAAGGAA GTACTTCTGT TTCCTGCCAT GAAACCCGAA
    GACAAGAAGG AGAATGTAGC AACCACTGAT ACACTGGAAA GCACAACAGT TGGCACTTCT
    GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878706.1
    CDS609..1934
    Translation

    Target ORF information:

    RefSeq Version XM_017023217.1
    Organism Homo sapiens(human)
    Definition Homo sapiens lysyl-tRNA synthetase 1 (KARS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017023217.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCCAATT CCAGAAATTA TAAATCAGAA GAAGAATTTA TTCATATTAA TAACAAACTG 
    CGTCGGGGAG ACATAATTGG AGTTCAGGGG AATCCTGGTA AAACCAAGAA GGGTGAGCTG
    AGCATCATTC CGTATGAGAT CACACTGCTG TCTCCCTGTT TGCATATGTT ACCTCATCTT
    CACTTTGGCC TCAAAGACAA GGAAACAAGG TATCGCCAGA GATACTTGGA CTTGATCCTG
    AATGACTTTG TGAGGCAGAA ATTTATCATC CGCTCTAAGA TCATCACATA TATAAGAAGT
    TTCTTAGATG AGCTGGGATT CCTAGAGATT GAAACTCCCA TGATGAACAT CATCCCAGGG
    GGAGCCGTGG CCAAGCCTTT CATCACTTAT CACAACGAGC TGGACATGAA CTTATATATG
    AGAATTGCTC CAGAACTCTA TCATAAGATG CTTGTGGTTG GTGGCATCGA CCGGGTTTAT
    GAAATTGGAC GCCAGTTCCG GAATGAGGGG ATTGATTTGA CGCACAATCC TGAGTTCACC
    ACCTGTGAGT TCTACATGGC CTATGCAGAC TATCACGATC TCATGGAAAT CACGGAGAAG
    ATGGTTTCAG GGATGGTGAA GCATATTACA GGCAGTTACA AGGTCACCTA CCACCCAGAT
    GGCCCAGAGG GCCAAGCCTA CGATGTTGAC TTCACCCCAC CCTTCCGGCG AATCAACATG
    GTAGAAGAGC TTGAGAAAGC CCTGGGGATG AAGCTGCCAG AAACGAACCT CTTTGAAACT
    GAAGAAACTC GCAAAATTCT TGATGATATC TGTGTGGCAA AAGCTGTTGA ATGCCCTCCA
    CCTCGGACCA CAGCCAGGCT CCTTGACAAG CTTGTTGGGG AGTTCCTGGA AGTGACTTGC
    ATCAATCCTA CATTCATCTG TGATCACCCA CAGATAATGA GCCCTTTGGC TAAATGGCAC
    CGCTCTAAAG AGGGTCTGAC TGAGCGCTTT GAGCTGTTTG TCATGAAGAA AGAGATATGC
    AATGCGTATA CTGAGCTGAA TGATCCCATG CGGCAGCGGC AGCTTTTTGA AGAACAGGCC
    AAGGCCAAGG CTGCAGGTGA TGATGAGGCC ATGTTCATAG ATGAAAACTT CTGTACTGCC
    CTGGAATATG GGCTGCCCCC CACAGCTGGC TGGGGCATGG GCATTGATCG AGTCGCCATG
    TTTCTCACGG ACTCCAACAA CATCAAGGAA GTACTTCTGT TTCCTGCCAT GAAACCCGAA
    GACAAGAAGG AGAATGTAGC AACCACTGAT ACACTGGAAA GCACAACAGT TGGCACTTCT
    GTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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