AGRN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGRN
Entrez Gene ID	375790
Full Name	agrin
Synonyms	AGRIN,CMS8,CMSPPD
General protein information	Preferred Names agrin Names agrin agrin proteoglycan
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.33
Summary	This gene encodes one of several proteins that are critical in the development of the neuromuscular junction (NMJ), as identified in mouse knock-out studies. The encoded protein contains several laminin G, Kazal type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur to add glycosaminoglycans and disulfide bonds. In one family with congenital myasthenic syndrome affecting limb-girdle muscles, a mutation in this gene was found. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015].
Disorder MIM:	103320
Disorder Html:	Myasthenic syndrome, congenital, 8, with pre- and postsynaptic defects, 615120 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305275.1	NP_001292204.1	agrin isoform 1 precursor
NM_001364727.2	NP_001351656.1	agrin isoform 3
NM_001364727.1	NP_001351656.1	agrin isoform 3
NM_001305275.2	NP_001292204.1	agrin isoform 1 precursor
NM_198576.4	NP_940978.2	agrin isoform 2 precursor
XM_005244749.3	XP_005244806.1	agrin isoform X1
NM_198576.3	NP_940978.2	agrin isoform 2 precursor
XM_011541429.2	XP_011539731.1	agrin isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AGRN	XP_005620416.1
Homo sapiens (human)	AGRN	NP_940978.2
Rattus norvegicus (Norway rat)	Agrn	NP_786930.1
Caenorhabditis elegans (roundworm)	agr-1	NP_001022152.3
Xenopus tropicalis (tropical clawed frog)	agrn	XP_002936430.2
	AGRN	XP_001088755.2
Mus musculus (house mouse)	Agrn	NP_067617.3
Gallus gallus (chicken)	AGRN	NP_990858.1
Pan troglodytes (chimpanzee)	LOC100609326	XP_003319002.1
Bos taurus (cattle)	AGRN	XP_005217262.1
Danio rerio (zebrafish)	hm:gc12	NP_001170923.1

Gene Ontology
Bibliography

Related articles in PubMed

JQ1 is a potential therapeutic option for COPD Patients with Agrin overexpression.
Xiao Z, Shu J, Zhou F, Han Y
American journal of physiology. Lung cellular and molecular physiology(2017 Dec)

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR, Hynes RO
Journal of proteome research16(8)3083-3091(2017 Aug)

C-terminal fragment of agrin (CAF) levels predict acute kidney injury after acute myocardial infarction.
Arampatzis S, Chalikias G, Devetzis V, Konstantinides S, Huynh-Do U, Tziakas D
BMC nephrology18(1)202(2017 Jun)

Agrin as a Mechanotransduction Signal Regulating YAP through the Hippo Pathway.
Chakraborty S, Njah K, Pobbati AV, Lim YB, Raju A, Lakshmanan M, Tergaonkar V, Lim CT, Hong W
Cell reports18(10)2464-2479(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Agrin promotes oncogenesis through YAP-dependent transcription.
Title: Agrin as a Mechanotransduction Signal Regulating YAP through the Hippo Pathway.

Agrin strongly promotes chondrocyte differentiation and cartilage formation in vivo.
Title: Agrin mediates chondrocyte homeostasis and requires both LRP4 and ?-dystroglycan to enhance cartilage formation in vitro and in vivo.

In patients suffering from severe sepsis and septic shock, serum levels of C-terminal agrin fragment were significantly associated with kidney function and the need for renal replacement therapy and were not influenced by severe septic conditions.
Title: C-terminal agrin fragment (CAF) reflects renal function in patients suffering from severe sepsis or septic shock.

Among 42 hip fractured patients (age 83.7+/-8.6 years, 76.2% women), sarcopenia was diagnosed in 7 individuals (16.7%). Serum C-terminal agrin fragment (CAF) levels were significantly higher in sarcopenic relative to non-sarcopenic patients.
Title: Serum levels of C-terminal agrin fragment (CAF) are associated with sarcopenia in older hip fractured patients.

Knockdown of agrin and perlecan promoted a decrease on cell migration and adhesion, and on resistance of cells to cisplatin.
Title: Agrin and perlecan mediate tumorigenic processes in oral squamous cell carcinoma.

The following AGRN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGRN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu79805	NM_001305275.2 Latest version!	Homo sapiens agrin (AGRN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu33428	XM_005244749.3 Latest version!	Homo sapiens agrin (AGRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu107768	NM_001364727.2 Latest version!	Homo sapiens agrin (AGRN), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu17750	NM_198576.4 Latest version!	Homo sapiens agrin (AGRN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu79802	XM_011541429.2 Latest version!	Homo sapiens agrin (AGRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu107768	NM_001364727.1	Homo sapiens agrin (AGRN), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu79805	NM_001305275.1	Homo sapiens agrin (AGRN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$779.50-$1091.30 $1559.00
OHu17750	NM_198576.3	Homo sapiens agrin (AGRN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGRN ( NM_001305275.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001292204.1 Homo sapiens agrin (AGRN), transcript variant 1, mRNA.

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CloneID	OHu79805
Clone ID Related Accession (Same CDS sequence)	NM_001305275.1 , NM_001305275.2
Accession Version	NM_001305275.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	agrin isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645608.30. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2154665, SAMN03465404 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC 
GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG 
GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG 
CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG 
GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC 
CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA 
CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG 
ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC 
TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC 
GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC 
CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA 
TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG 
CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG 
CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC 
GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC 
CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT 
CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT 
GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC 
GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC 
CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG 
TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC 
TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC 
TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG 
TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT 
GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT 
GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT 
ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC 
CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG 
GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG 
CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG 
ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT 
CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC 
AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG 
GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT 
GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC 
GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA 
GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA 
GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT 
GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA 
GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT 
TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC 
CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC 
CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG 
CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT 
CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC 
TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC 
TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT 
GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA 
ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA 
TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC 
CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC 
TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG 
GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT 
GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT 
GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC 
GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG 
CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG 
GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA 
CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA 
GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC 
CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC 
CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG 
CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC 
ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG 
TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG 
ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA 
CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC 
GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC 
AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC 
TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC 
CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC 
CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG 
GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC 
CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT 
CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG 
GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC 
CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC 
CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC 
GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC 
GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG 
GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG 
GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC 
AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG 
AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC 
GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG 
GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC 
CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG 
GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGAAATCCC GCAAGGTTCC GCACACCGTC 
CTCAACCTGA AGGAGCCGCT CTACGTAGGG GGCGCTCCCG ACTTCAGCAA GCTGGCCCGT 
GCTGCTGCCG TGTCCTCTGG CTTCGACGGT GCCATCCAGC TGGTCTCCCT CGGAGGCCGC 
CAGCTGCTGA CCCCGGAGCA CGTGCTGCGG CAGGTGGACG TCACGTCCTT TGCAGGTCAC 
CCCTGCACCC GGGCCTCAGG CCACCCCTGC CTCAATGGGG CCTCCTGCGT CCCGAGGGAG 
GCTGCCTATG TGTGCCTGTG TCCCGGGGGA TTCTCAGGAC CGCACTGCGA GAAGGGGCTG 
GTGGAGAAGT CAGCGGGGGA CGTGGATACC TTGGCCTTTG ACGGGCGGAC CTTTGTCGAG 
TACCTCAACG CTGTGACCGA GAGCGAACTG GCCAATGAGA TCCCCGTCCC CGAAACTCTG 
GATTCCGGGG CCCTTCACAG CGAGAAGGCA CTGCAGAGCA ACCACTTTGA ACTGAGCCTG 
CGCACTGAGG CCACGCAGGG GCTGGTGCTC TGGAGTGGCA AGGCCACGGA GCGGGCAGAC 
TATGTGGCAC TGGCCATTGT GGACGGGCAC CTGCAACTGA GCTACAACCT GGGCTCCCAG 
CCCGTGGTGC TGCGTTCCAC CGTGCCCGTC AACACCAACC GCTGGTTGCG GGTCGTGGCA 
CATAGGGAGC AGAGGGAAGG TTCCCTGCAG GTGGGCAATG AGGCCCCTGT GACCGGCTCC 
TCCCCGCTGG GCGCCACGCA GCTGGACACT GATGGAGCCC TGTGGCTTGG GGGCCTGCCG 
GAGCTGCCCG TGGGCCCAGC ACTGCCCAAG GCCTACGGCA CAGGCTTTGT GGGCTGCTTG 
CGGGACGTGG TGGTGGGCCG GCACCCGCTG CACCTGCTGG AGGACGCCGT CACCAAGCCA 
GAGCTGCGGC CCTGCCCCAC CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292204.1
CDS	51..6257
Translation	MAGRSHPGPLRPLLPLLVVAACVLPGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESELANEIPVPETLDSGALHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	NM_001305275.1
Organism	Homo sapiens(human)
Definition	Homo sapiens agrin (AGRN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305275.1

ORF Insert Sequence:

ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC 
GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG 
GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG 
CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG 
GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC 
CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA 
CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG 
ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC 
TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC 
GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC 
CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA 
TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG 
CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG 
CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC 
GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC 
CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT 
CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT 
GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC 
GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC 
CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG 
TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC 
TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC 
TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG 
TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT 
GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT 
GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT 
ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC 
CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG 
GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG 
CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG 
ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT 
CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC 
AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG 
GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT 
GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC 
GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA 
GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA 
GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT 
GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA 
GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT 
TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC 
CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC 
CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG 
CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT 
CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC 
TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC 
TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT 
GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA 
ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA 
TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC 
CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC 
TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG 
GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT 
GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT 
GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC 
GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG 
CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG 
GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA 
CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA 
GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC 
CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC 
CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG 
CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC 
ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG 
TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG 
ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA 
CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC 
GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC 
AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC 
TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC 
CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC 
CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG 
GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC 
CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT 
CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG 
GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC 
CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC 
CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC 
GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC 
GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG 
GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG 
GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC 
AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG 
AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC 
GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG 
GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC 
CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG 
GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGAAATCCC GCAAGGTTCC GCACACCGTC 
CTCAACCTGA AGGAGCCGCT CTACGTAGGG GGCGCTCCCG ACTTCAGCAA GCTGGCCCGT 
GCTGCTGCCG TGTCCTCTGG CTTCGACGGT GCCATCCAGC TGGTCTCCCT CGGAGGCCGC 
CAGCTGCTGA CCCCGGAGCA CGTGCTGCGG CAGGTGGACG TCACGTCCTT TGCAGGTCAC 
CCCTGCACCC GGGCCTCAGG CCACCCCTGC CTCAATGGGG CCTCCTGCGT CCCGAGGGAG 
GCTGCCTATG TGTGCCTGTG TCCCGGGGGA TTCTCAGGAC CGCACTGCGA GAAGGGGCTG 
GTGGAGAAGT CAGCGGGGGA CGTGGATACC TTGGCCTTTG ACGGGCGGAC CTTTGTCGAG 
TACCTCAACG CTGTGACCGA GAGCGAACTG GCCAATGAGA TCCCCGTCCC CGAAACTCTG 
GATTCCGGGG CCCTTCACAG CGAGAAGGCA CTGCAGAGCA ACCACTTTGA ACTGAGCCTG 
CGCACTGAGG CCACGCAGGG GCTGGTGCTC TGGAGTGGCA AGGCCACGGA GCGGGCAGAC 
TATGTGGCAC TGGCCATTGT GGACGGGCAC CTGCAACTGA GCTACAACCT GGGCTCCCAG 
CCCGTGGTGC TGCGTTCCAC CGTGCCCGTC AACACCAACC GCTGGTTGCG GGTCGTGGCA 
CATAGGGAGC AGAGGGAAGG TTCCCTGCAG GTGGGCAATG AGGCCCCTGT GACCGGCTCC 
TCCCCGCTGG GCGCCACGCA GCTGGACACT GATGGAGCCC TGTGGCTTGG GGGCCTGCCG 
GAGCTGCCCG TGGGCCCAGC ACTGCCCAAG GCCTACGGCA CAGGCTTTGT GGGCTGCTTG 
CGGGACGTGG TGGTGGGCCG GCACCCGCTG CACCTGCTGG AGGACGCCGT CACCAAGCCA 
GAGCTGCGGC CCTGCCCCAC CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( NM_001305275.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001292204.1 Homo sapiens agrin (AGRN), transcript variant 1, mRNA.

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CloneID	OHu79805
Clone ID Related Accession (Same CDS sequence)	NM_001305275.1 , NM_001305275.2
Accession Version	NM_001305275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	agrin isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645608.30. On Dec 15, 2018 this sequence version replaced NM_001305275.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2154665, SAMN03465404 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC 
GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG 
GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG 
CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG 
GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC 
CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA 
CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG 
ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC 
TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC 
GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC 
CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA 
TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG 
CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG 
CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC 
GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC 
CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT 
CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT 
GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC 
GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC 
CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG 
TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC 
TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC 
TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG 
TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT 
GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT 
GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT 
ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC 
CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG 
GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG 
CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG 
ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT 
CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC 
AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG 
GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT 
GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC 
GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA 
GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA 
GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT 
GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA 
GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT 
TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC 
CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC 
CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG 
CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT 
CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC 
TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC 
TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT 
GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA 
ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA 
TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC 
CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC 
TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG 
GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT 
GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT 
GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC 
GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG 
CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG 
GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA 
CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA 
GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC 
CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC 
CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG 
CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC 
ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG 
TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG 
ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA 
CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC 
GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC 
AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC 
TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC 
CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC 
CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG 
GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC 
CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT 
CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG 
GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC 
CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC 
CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC 
GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC 
GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG 
GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG 
GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC 
AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG 
AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC 
GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG 
GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC 
CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG 
GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGAAATCCC GCAAGGTTCC GCACACCGTC 
CTCAACCTGA AGGAGCCGCT CTACGTAGGG GGCGCTCCCG ACTTCAGCAA GCTGGCCCGT 
GCTGCTGCCG TGTCCTCTGG CTTCGACGGT GCCATCCAGC TGGTCTCCCT CGGAGGCCGC 
CAGCTGCTGA CCCCGGAGCA CGTGCTGCGG CAGGTGGACG TCACGTCCTT TGCAGGTCAC 
CCCTGCACCC GGGCCTCAGG CCACCCCTGC CTCAATGGGG CCTCCTGCGT CCCGAGGGAG 
GCTGCCTATG TGTGCCTGTG TCCCGGGGGA TTCTCAGGAC CGCACTGCGA GAAGGGGCTG 
GTGGAGAAGT CAGCGGGGGA CGTGGATACC TTGGCCTTTG ACGGGCGGAC CTTTGTCGAG 
TACCTCAACG CTGTGACCGA GAGCGAACTG GCCAATGAGA TCCCCGTCCC CGAAACTCTG 
GATTCCGGGG CCCTTCACAG CGAGAAGGCA CTGCAGAGCA ACCACTTTGA ACTGAGCCTG 
CGCACTGAGG CCACGCAGGG GCTGGTGCTC TGGAGTGGCA AGGCCACGGA GCGGGCAGAC 
TATGTGGCAC TGGCCATTGT GGACGGGCAC CTGCAACTGA GCTACAACCT GGGCTCCCAG 
CCCGTGGTGC TGCGTTCCAC CGTGCCCGTC AACACCAACC GCTGGTTGCG GGTCGTGGCA 
CATAGGGAGC AGAGGGAAGG TTCCCTGCAG GTGGGCAATG AGGCCCCTGT GACCGGCTCC 
TCCCCGCTGG GCGCCACGCA GCTGGACACT GATGGAGCCC TGTGGCTTGG GGGCCTGCCG 
GAGCTGCCCG TGGGCCCAGC ACTGCCCAAG GCCTACGGCA CAGGCTTTGT GGGCTGCTTG 
CGGGACGTGG TGGTGGGCCG GCACCCGCTG CACCTGCTGG AGGACGCCGT CACCAAGCCA 
GAGCTGCGGC CCTGCCCCAC CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292204.1
CDS	54..6260
Translation	MAGRSHPGPLRPLLPLLVVAACVLPGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESELANEIPVPETLDSGALHSEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	NM_001305275.2
Organism	Homo sapiens(human)
Definition	Homo sapiens agrin (AGRN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305275.2

ORF Insert Sequence:

ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC 
GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG 
GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG 
CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG 
GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC 
CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA 
CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG 
ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC 
TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC 
GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC 
CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA 
TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG 
CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG 
CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC 
GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC 
CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT 
CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT 
GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC 
GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC 
CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG 
TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC 
TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC 
TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG 
TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT 
GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT 
GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT 
ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC 
CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG 
GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG 
CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG 
ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT 
CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC 
AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG 
GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT 
GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC 
GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA 
GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA 
GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT 
GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA 
GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT 
TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC 
CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC 
CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG 
CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT 
CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC 
TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC 
TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT 
GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA 
ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA 
TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC 
CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC 
TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG 
GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT 
GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT 
GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC 
GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG 
CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG 
GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA 
CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA 
GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC 
CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC 
CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG 
CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC 
ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG 
TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG 
ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA 
CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC 
GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC 
AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC 
TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC 
CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC 
CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG 
GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC 
CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT 
CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG 
GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC 
CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC 
CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC 
GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC 
GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG 
GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG 
GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC 
AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG 
AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC 
GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG 
GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC 
CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG 
GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGAAATCCC GCAAGGTTCC GCACACCGTC 
CTCAACCTGA AGGAGCCGCT CTACGTAGGG GGCGCTCCCG ACTTCAGCAA GCTGGCCCGT 
GCTGCTGCCG TGTCCTCTGG CTTCGACGGT GCCATCCAGC TGGTCTCCCT CGGAGGCCGC 
CAGCTGCTGA CCCCGGAGCA CGTGCTGCGG CAGGTGGACG TCACGTCCTT TGCAGGTCAC 
CCCTGCACCC GGGCCTCAGG CCACCCCTGC CTCAATGGGG CCTCCTGCGT CCCGAGGGAG 
GCTGCCTATG TGTGCCTGTG TCCCGGGGGA TTCTCAGGAC CGCACTGCGA GAAGGGGCTG 
GTGGAGAAGT CAGCGGGGGA CGTGGATACC TTGGCCTTTG ACGGGCGGAC CTTTGTCGAG 
TACCTCAACG CTGTGACCGA GAGCGAACTG GCCAATGAGA TCCCCGTCCC CGAAACTCTG 
GATTCCGGGG CCCTTCACAG CGAGAAGGCA CTGCAGAGCA ACCACTTTGA ACTGAGCCTG 
CGCACTGAGG CCACGCAGGG GCTGGTGCTC TGGAGTGGCA AGGCCACGGA GCGGGCAGAC 
TATGTGGCAC TGGCCATTGT GGACGGGCAC CTGCAACTGA GCTACAACCT GGGCTCCCAG 
CCCGTGGTGC TGCGTTCCAC CGTGCCCGTC AACACCAACC GCTGGTTGCG GGTCGTGGCA 
CATAGGGAGC AGAGGGAAGG TTCCCTGCAG GTGGGCAATG AGGCCCCTGT GACCGGCTCC 
TCCCCGCTGG GCGCCACGCA GCTGGACACT GATGGAGCCC TGTGGCTTGG GGGCCTGCCG 
GAGCTGCCCG TGGGCCCAGC ACTGCCCAAG GCCTACGGCA CAGGCTTTGT GGGCTGCTTG 
CGGGACGTGG TGGTGGGCCG GCACCCGCTG CACCTGCTGG AGGACGCCGT CACCAAGCCA 
GAGCTGCGGC CCTGCCCCAC CCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_005244749.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005244806.1 Homo sapiens agrin (AGRN), transcript variant X1, mRNA.

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CloneID	OHu33428
Clone ID Related Accession (Same CDS sequence)	XM_005244749.3
Accession Version	XM_005244749.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	agrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005244749.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC 
GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG 
GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG 
CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG 
GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC 
CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA 
CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG 
ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC 
TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC 
GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC 
CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA 
TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG 
CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG 
CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC 
GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC 
CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT 
CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT 
GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC 
GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC 
CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG 
TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC 
TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC 
TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG 
TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT 
GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT 
GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT 
ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC 
CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG 
GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG 
CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG 
ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT 
CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC 
AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG 
GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT 
GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC 
GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA 
GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA 
GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT 
GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA 
GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT 
TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC 
CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC 
CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG 
CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT 
CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC 
TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC 
TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT 
GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA 
ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA 
TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC 
CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC 
TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG 
GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT 
GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT 
GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC 
GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG 
CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG 
GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA 
CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA 
GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC 
CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC 
CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG 
CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC 
ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG 
TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG 
ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA 
CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC 
GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC 
AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC 
TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC 
CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC 
CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG 
GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC 
CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT 
CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG 
GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC 
CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC 
CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC 
GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC 
GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG 
GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG 
GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC 
AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG 
AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC 
GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG 
GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC 
CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG 
GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGAAATCCC GCAAGGTTCC GCACACCGTC 
CTCAACCTGA AGGAGCCGCT CTACGTAGGG GGCGCTCCCG ACTTCAGCAA GCTGGCCCGT 
GCTGCTGCCG TGTCCTCTGG CTTCGACGGT GCCATCCAGC TGGTCTCCCT CGGAGGCCGC 
CAGCTGCTGA CCCCGGAGCA CGTGCTGCGG CAGGTGGACG TCACGTCCTT TGCAGGTCAC 
CCCTGCACCC GGGCCTCAGG CCACCCCTGC CTCAATGGGG CCTCCTGCGT CCCGAGGGAG 
GCTGCCTATG TGTGCCTGTG TCCCGGGGGA TTCTCAGGAC CGCACTGCGA GAAGGGGCTG 
GTGGAGAAGT CAGCGGGGGA CGTGGATACC TTGGCCTTTG ACGGGCGGAC CTTTGTCGAG 
TACCTCAACG CTGTGACCGA GAGCGAGAAG GCACTGCAGA GCAACCACTT TGAACTGAGC 
CTGCGCACTG AGGCCACGCA GGGGCTGGTG CTCTGGAGTG GCAAGGCCAC GGAGCGGGCA 
GACTATGTGG CACTGGCCAT TGTGGACGGG CACCTGCAAC TGAGCTACAA CCTGGGCTCC 
CAGCCCGTGG TGCTGCGTTC CACCGTGCCC GTCAACACCA ACCGCTGGTT GCGGGTCGTG 
GCACATAGGG AGCAGAGGGA AGGTTCCCTG CAGGTGGGCA ATGAGGCCCC TGTGACCGGC 
TCCTCCCCGC TGGGCGCCAC GCAGCTGGAC ACTGATGGAG CCCTGTGGCT TGGGGGCCTG 
CCGGAGCTGC CCGTGGGCCC AGCACTGCCC AAGGCCTACG GCACAGGCTT TGTGGGCTGC 
TTGCGGGACG TGGTGGTGGG CCGGCACCCG CTGCACCTGC TGGAGGACGC CGTCACCAAG 
CCAGAGCTGC GGCCCTGCCC CACCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005244806.1
CDS	72..6221
Translation	MAGRSHPGPLRPLLPLLVVAACVLPGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	XM_005244749.3
Organism	Homo sapiens(human)
Definition	Homo sapiens agrin (AGRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005244749.3

ORF Insert Sequence:

ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC 
GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG 
GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG 
CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG 
GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC 
CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA 
CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG 
ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC 
TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC 
GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC 
CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA 
TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG 
CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG 
CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC 
GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC 
CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT 
CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT 
GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC 
GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC 
CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG 
TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC 
TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC 
TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG 
TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT 
GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT 
GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT 
ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC 
CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG 
GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG 
CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG 
ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT 
CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC 
AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG 
GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT 
GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC 
GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA 
GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA 
GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT 
GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA 
GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT 
TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC 
CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC 
CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG 
CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT 
CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC 
TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC 
TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT 
GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA 
ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA 
TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC 
CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC 
TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG 
GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT 
GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT 
GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC 
GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG 
CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG 
GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA 
CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA 
GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC 
CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC 
CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG 
CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC 
ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG 
TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG 
ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA 
CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC 
GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC 
AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC 
TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC 
CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC 
CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG 
GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC 
CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT 
CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG 
GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC 
CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC 
CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC 
GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC 
GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG 
GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG 
GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC 
AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG 
AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC 
GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG 
GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC 
CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG 
GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGAAATCCC GCAAGGTTCC GCACACCGTC 
CTCAACCTGA AGGAGCCGCT CTACGTAGGG GGCGCTCCCG ACTTCAGCAA GCTGGCCCGT 
GCTGCTGCCG TGTCCTCTGG CTTCGACGGT GCCATCCAGC TGGTCTCCCT CGGAGGCCGC 
CAGCTGCTGA CCCCGGAGCA CGTGCTGCGG CAGGTGGACG TCACGTCCTT TGCAGGTCAC 
CCCTGCACCC GGGCCTCAGG CCACCCCTGC CTCAATGGGG CCTCCTGCGT CCCGAGGGAG 
GCTGCCTATG TGTGCCTGTG TCCCGGGGGA TTCTCAGGAC CGCACTGCGA GAAGGGGCTG 
GTGGAGAAGT CAGCGGGGGA CGTGGATACC TTGGCCTTTG ACGGGCGGAC CTTTGTCGAG 
TACCTCAACG CTGTGACCGA GAGCGAGAAG GCACTGCAGA GCAACCACTT TGAACTGAGC 
CTGCGCACTG AGGCCACGCA GGGGCTGGTG CTCTGGAGTG GCAAGGCCAC GGAGCGGGCA 
GACTATGTGG CACTGGCCAT TGTGGACGGG CACCTGCAAC TGAGCTACAA CCTGGGCTCC 
CAGCCCGTGG TGCTGCGTTC CACCGTGCCC GTCAACACCA ACCGCTGGTT GCGGGTCGTG 
GCACATAGGG AGCAGAGGGA AGGTTCCCTG CAGGTGGGCA ATGAGGCCCC TGTGACCGGC 
TCCTCCCCGC TGGGCGCCAC GCAGCTGGAC ACTGATGGAG CCCTGTGGCT TGGGGGCCTG 
CCGGAGCTGC CCGTGGGCCC AGCACTGCCC AAGGCCTACG GCACAGGCTT TGTGGGCTGC 
TTGCGGGACG TGGTGGTGGG CCGGCACCCG CTGCACCTGC TGGAGGACGC CGTCACCAAG 
CCAGAGCTGC GGCCCTGCCC CACCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( NM_001364727.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001351656.1 Homo sapiens agrin (AGRN), transcript variant 3, mRNA.

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CloneID	OHu107768
Clone ID Related Accession (Same CDS sequence)	NM_001364727.2 , NM_001364727.1
Accession Version	NM_001364727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	agrin isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645608.30. On Jun 1, 2019 this sequence version replaced NM_001364727.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGCCTCCGC TGCCGCTGGC GCGGGACACC CGGCAGCCGC CTGGCGCCTC CCTGCTGGTG 
CGAGGCTTCA TGGTGCCCTG CAACGCCTGC CTGATCCTGC TGGCCACCGC CACGCTCGGC 
TTCGCGGTGC TGCTGTTCCT CAACAACTAT AAACCCGGGA CCCACTTCAC TCCAGTGCCT 
CCGACGCCTC CTGATGCGTG CCGGGGAATG CTGTGCGGCT TCGGCGCCGT GTGCGAGCCC 
AACGCGGAGG GGCCGGGCCG GGCGTCCTGC GTCTGCAAGA AGAGCCCGTG CCCCAGCGTG 
GTGGCGCCTG TGTGTGGGTC GGACGCCTCC ACCTACAGCA ACGAATGCGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTGCTCAGCC GCGGGCCGTG CGGCTCGCGG 
GACCCCTGCT CCAACGTGAC CTGCAGCTTC GGCAGCACCT GTGCGCGCTC GGCCGACGGG 
CTGACGGCCT CGTGCCTGTG CCCCGCGACC TGCCGTGGCG CCCCCGAGGG GACCGTCTGC 
GGCAGCGACG GCGCCGACTA CCCCGGCGAG TGCCAGCTCC TGCGCCGCGC CTGCGCCCGC 
CAGGAGAATG TCTTCAAGAA GTTCGACGGC CCTTGTGACC CCTGTCAGGG CGCCCTCCCT 
GACCCGAGCC GCAGCTGCCG TGTGAACCCG CGCACGCGGC GCCCTGAGAT GCTCCTACGG 
CCCGAGAGCT GCCCTGCCCG GCAGGCGCCA GTGTGTGGGG ACGACGGAGT CACCTACGAA 
AACGACTGTG TCATGGGCCG ATCGGGGGCC GCCCGGGGTC TCCTCCTGCA GAAAGTGCGC 
TCCGGCCAGT GCCAGGGTCG AGACCAGTGC CCGGAGCCCT GCCGGTTCAA TGCCGTGTGC 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGCGACCGCG TCACCTGTGA CGGGGCCTAC 
AGGCCCGTGT GTGCCCAGGA CGGGCGCACG TATGACAGTG ATTGCTGGCG GCAGCAGGCT 
GAGTGCCGGC AGCAGCGTGC CATCCCCAGC AAGCACCAGG GCCCGTGTGA CCAGGCCCCG 
TCCCCATGCC TCGGGGTGCA GTGTGCATTT GGGGCGACGT GTGCTGTGAA GAACGGGCAG 
GCAGCGTGTG AATGCCTGCA GGCGTGCTCG AGCCTCTACG ATCCTGTGTG CGGCAGCGAC 
GGCGTCACAT ACGGCAGCGC GTGCGAGCTG GAGGCCACGG CCTGTACCCT CGGGCGGGAG 
ATCCAGGTGG CGCGCAAAGG ACCCTGTGAC CGCTGCGGGC AGTGCCGCTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG CTGCGTGTGC CCCTCTGAAT GCGTGGCTTT GGCCCAGCCC 
GTGTGTGGCT CCGACGGGCA CACGTACCCC AGCGAGTGCA TGCTGCACGT GCACGCCTGC 
ACACACCAGA TCAGCCTGCA CGTGGCCTCA GCTGGACCCT GTGAGACCTG TGGAGATGCC 
GTGTGTGCTT TTGGGGCTGT GTGCTCCGCA GGGCAGTGTG TGTGTCCCCG GTGTGAGCAC 
CCCCCGCCCG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACGGCAGTGC CTGCGAGCTA 
CGGGAAGCCG CCTGCCTCCA GCAGACACAG ATCGAGGAGG CCCGGGCAGG GCCGTGCGAG 
CAGGCCGAGT GCGGTTCCGG AGGCTCTGGC TCTGGGGAGG ACGGTGACTG TGAGCAGGAG 
CTGTGCCGGC AGCGCGGTGG CATCTGGGAC GAGGACTCGG AGGACGGGCC GTGTGTCTGT 
GACTTCAGCT GCCAGAGTGT CCCAGGCAGC CCGGTGTGCG GCTCAGATGG GGTCACCTAC 
AGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAGTCAC AGCGAGGGCT CTACGTAGCG 
GCCCAGGGAG CCTGCCGAGG CCCCACCTTC GCCCCGCTGC CGCCTGTGGC CCCCTTACAC 
TGTGCCCAGA CGCCCTACGG CTGCTGCCAG GACAATATCA CCGCAGCCCG GGGCGTGGGC 
CTGGCTGGCT GCCCCAGTGC CTGCCAGTGC AACCCCCATG GCTCTTACGG CGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCCTGCCGC CCAGGTGTGG GGGGCCTCAG GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTTCGAGGC ATCGTCACCG ATGGCCGGAG TGGCTGTACA 
CCCTGCAGCT GTGATCCCCA AGGCGCCGTG CGGGATGACT GTGAGCAGAT GACGGGGCTG 
TGCTCGTGTA AGCCCGGGGT GGCTGGACCC AAGTGTGGGC AGTGTCCAGA CGGCCGTGCC 
CTGGGCCCCG CGGGCTGTGA AGCTGACGCT TCTGCGCCTG CGACCTGTGC GGAGATGCGC 
TGTGAGTTCG GTGCGCGGTG CGTGGAGGAG TCTGGCTCAG CCCACTGTGT CTGCCCGATG 
CTCACCTGTC CAGAGGCCAA CGCTACCAAG GTCTGTGGGT CAGATGGAGT CACATACGGC 
AACGAGTGTC AGCTGAAGAC CATCGCCTGC CGCCAGGGCC TGCAAATCTC TATCCAGAGC 
CTGGGCCCGT GCCAGGAGGC TGTTGCTCCC AGCACTCACC CGACATCTGC CTCCGTGACT 
GTGACCACCC CAGGGCTCCT CCTGAGCCAG GCACTGCCGG CCCCCCCCGG CGCCCTCCCC 
CTGGCTCCCA GCAGTACCGC ACACAGCCAG ACCACCCCTC CGCCCTCATC ACGACCTCGG 
ACCACTGCCA GCGTCCCCAG GACCACCGTG TGGCCCGTGC TGACGGTGCC CCCCACGGCA 
CCCTCCCCTG CACCCAGCCT GGTGGCGTCC GCCTTTGGTG AATCTGGCAG CACTGATGGA 
AGCAGCGATG AGGAACTGAG CGGGGACCAG GAGGCCAGTG GGGGTGGCTC TGGGGGGCTC 
GAGCCCTTGG AGGGCAGCAG CGTGGCCACC CCTGGGCCAC CTGTCGAGAG GGCTTCCTGC 
TACAACTCCG CGTTGGGCTG CTGCTCTGAT GGGAAGACGC CCTCGCTGGA CGCAGAGGGC 
TCCAACTGCC CCGCCACCAA GGTGTTCCAG GGCGTCCTGG AGCTGGAGGG CGTCGAGGGC 
CAGGAGCTGT TCTACACGCC CGAGATGGCT GACCCCAAGT CAGAACTGTT CGGGGAGACA 
GCCAGGAGCA TTGAGAGCAC CCTGGACGAC CTCTTCCGGA ATTCAGACGT CAAGAAGGAT 
TTTCGGAGTG TCCGCTTGCG GGACCTGGGG CCCGGCAAAT CCGTCCGCGC CATTGTGGAT 
GTGCACTTTG ACCCCACCAC AGCCTTCAGG GCACCCGACG TGGCCCGGGC CCTGCTCCGG 
CAGATCCAGG TGTCCAGGCG CCGGTCCTTG GGGGTGAGGC GGCCGCTGCA GGAGCACGTG 
CGATTTATGG ACTTTGACTG GTTTCCTGCG TTTATCACGG GGGCCACGTC AGGAGCCATT 
GCTGCGGGAG CCACGGCCAG AGCCACCACT GCATCGCGCC TGCCGTCCTC TGCTGTGACC 
CCTCGGGCCC CGCACCCCAG TCACACAAGC CAGCCCGTTG CCAAGACCAC GGCAGCCCCC 
ACCACACGTC GGCCCCCCAC CACTGCCCCC AGCCGTGTGC CCGGACGTCG GCCCCCGGCC 
CCCCAGCAGC CTCCAAAGCC CTGTGACTCA CAGCCCTGCT TCCACGGGGG GACCTGCCAG 
GACTGGGCAT TGGGCGGGGG CTTCACCTGC AGCTGCCCGG CAGGCAGGGG AGGCGCCGTC 
TGTGAGAAGG TGCTTGGCGC CCCTGTGCCG GCCTTCGAGG GCCGCTCCTT CCTGGCCTTC 
CCCACTCTCC GCGCCTACCA CACGCTGCGC CTGGCACTGG AATTCCGGGC GCTGGAGCCT 
CAGGGGCTGC TGCTGTACAA TGGCAACGCC CGGGGCAAGG ACTTCCTGGC ATTGGCGCTG 
CTAGATGGCC GCGTGCAGCT CAGGTTTGAC ACAGGTTCGG GGCCGGCGGT GCTGACCAGT 
GCCGTGCCGG TAGAGCCGGG CCAGTGGCAC CGCCTGGAGC TGTCCCGGCA CTGGCGCCGG 
GGCACCCTCT CGGTGGATGG TGAGACCCCT GTTCTGGGCG AGAGTCCCAG TGGCACCGAC 
GGCCTCAACC TGGACACAGA CCTCTTTGTG GGCGGCGTAC CCGAGGACCA GGCTGCCGTG 
GCGCTGGAGC GGACCTTCGT GGGCGCCGGC CTGAGGGGGT GCATCCGTTT GCTGGACGTC 
AACAACCAGC GCCTGGAGCT TGGCATTGGG CCGGGGGCTG CCACCCGAGG CTCTGGCGTG 
GGCGAGTGCG GGGACCACCC CTGCCTGCCC AACCCCTGCC ATGGCGGGGC CCCATGCCAG 
AACCTGGAGG CTGGAAGGTT CCATTGCCAG TGCCCGCCCG GCCGCGTCGG ACCAACCTGT 
GCCGATGAGA AGAGCCCCTG CCAGCCCAAC CCCTGCCATG GGGCGGCGCC CTGCCGTGTG 
CTGCCCGAGG GTGGTGCTCA GTGCGAGTGC CCCCTGGGGC GTGAGGGCAC CTTCTGCCAG 
ACAGCCTCGG GGCAGGACGG CTCTGGGCCC TTCCTGGCTG ACTTCAACGG CTTCTCCCAC 
CTGGAGCTGA GAGGCCTGCA CACCTTTGCA CGGGACCTGG GGGAGAAGAT GGCGCTGGAG 
GTCGTGTTCC TGGCACGAGG CCCCAGCGGC CTCCTGCTCT ACAACGGGCA GAAGACGGAC 
GGCAAGGGGG ACTTCGTGTC GCTGGCACTG CGGGACCGCC GCCTGGAGTT CCGCTACGAC 
CTGGGCAAGG GGGCAGCGGT CATCAGGAGC AGGGAGCCAG TCACCCTGGG AGCCTGGACC 
AGGGTCTCAC TGGAGCGAAA CGGCCGCAAG GGTGCCCTGC GTGTGGGCGA CGGCCCCCGT 
GTGTTGGGGG AGTCCCCGAA ATCCCGCAAG GTTCCGCACA CCGTCCTCAA CCTGAAGGAG 
CCGCTCTACG TAGGGGGCGC TCCCGACTTC AGCAAGCTGG CCCGTGCTGC TGCCGTGTCC 
TCTGGCTTCG ACGGTGCCAT CCAGCTGGTC TCCCTCGGAG GCCGCCAGCT GCTGACCCCG 
GAGCACGTGC TGCGGCAGGT GGACGTCACG TCCTTTGCAG GTCACCCCTG CACCCGGGCC 
TCAGGCCACC CCTGCCTCAA TGGGGCCTCC TGCGTCCCGA GGGAGGCTGC CTATGTGTGC 
CTGTGTCCCG GGGGATTCTC AGGACCGCAC TGCGAGAAGG GGCTGGTGGA GAAGTCAGCG 
GGGGACGTGG ATACCTTGGC CTTTGACGGG CGGACCTTTG TCGAGTACCT CAACGCTGTG 
ACCGAGAGCG AGAAGGCACT GCAGAGCAAC CACTTTGAAC TGAGCCTGCG CACTGAGGCC 
ACGCAGGGGC TGGTGCTCTG GAGTGGCAAG GCCACGGAGC GGGCAGACTA TGTGGCACTG 
GCCATTGTGG ACGGGCACCT GCAACTGAGC TACAACCTGG GCTCCCAGCC CGTGGTGCTG 
CGTTCCACCG TGCCCGTCAA CACCAACCGC TGGTTGCGGG TCGTGGCACA TAGGGAGCAG 
AGGGAAGGTT CCCTGCAGGT GGGCAATGAG GCCCCTGTGA CCGGCTCCTC CCCGCTGGGC 
GCCACGCAGC TGGACACTGA TGGAGCCCTG TGGCTTGGGG GCCTGCCGGA GCTGCCCGTG 
GGCCCAGCAC TGCCCAAGGC CTACGGCACA GGCTTTGTGG GCTGCTTGCG GGACGTGGTG 
GTGGGCCGGC ACCCGCTGCA CCTGCTGGAG GACGCCGTCA CCAAGCCAGA GCTGCGGCCC 
TGCCCCACCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351656.1
CDS	564..6398
Translation	MPPLPLARDTRQPPGASLLVRGFMVPCNACLILLATATLGFAVLLFLNNYKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	NM_001364727.2
Organism	Homo sapiens(human)
Definition	Homo sapiens agrin (AGRN), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364727.2

ORF Insert Sequence:

ATGCCTCCGC TGCCGCTGGC GCGGGACACC CGGCAGCCGC CTGGCGCCTC CCTGCTGGTG 
CGAGGCTTCA TGGTGCCCTG CAACGCCTGC CTGATCCTGC TGGCCACCGC CACGCTCGGC 
TTCGCGGTGC TGCTGTTCCT CAACAACTAT AAACCCGGGA CCCACTTCAC TCCAGTGCCT 
CCGACGCCTC CTGATGCGTG CCGGGGAATG CTGTGCGGCT TCGGCGCCGT GTGCGAGCCC 
AACGCGGAGG GGCCGGGCCG GGCGTCCTGC GTCTGCAAGA AGAGCCCGTG CCCCAGCGTG 
GTGGCGCCTG TGTGTGGGTC GGACGCCTCC ACCTACAGCA ACGAATGCGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTGCTCAGCC GCGGGCCGTG CGGCTCGCGG 
GACCCCTGCT CCAACGTGAC CTGCAGCTTC GGCAGCACCT GTGCGCGCTC GGCCGACGGG 
CTGACGGCCT CGTGCCTGTG CCCCGCGACC TGCCGTGGCG CCCCCGAGGG GACCGTCTGC 
GGCAGCGACG GCGCCGACTA CCCCGGCGAG TGCCAGCTCC TGCGCCGCGC CTGCGCCCGC 
CAGGAGAATG TCTTCAAGAA GTTCGACGGC CCTTGTGACC CCTGTCAGGG CGCCCTCCCT 
GACCCGAGCC GCAGCTGCCG TGTGAACCCG CGCACGCGGC GCCCTGAGAT GCTCCTACGG 
CCCGAGAGCT GCCCTGCCCG GCAGGCGCCA GTGTGTGGGG ACGACGGAGT CACCTACGAA 
AACGACTGTG TCATGGGCCG ATCGGGGGCC GCCCGGGGTC TCCTCCTGCA GAAAGTGCGC 
TCCGGCCAGT GCCAGGGTCG AGACCAGTGC CCGGAGCCCT GCCGGTTCAA TGCCGTGTGC 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGCGACCGCG TCACCTGTGA CGGGGCCTAC 
AGGCCCGTGT GTGCCCAGGA CGGGCGCACG TATGACAGTG ATTGCTGGCG GCAGCAGGCT 
GAGTGCCGGC AGCAGCGTGC CATCCCCAGC AAGCACCAGG GCCCGTGTGA CCAGGCCCCG 
TCCCCATGCC TCGGGGTGCA GTGTGCATTT GGGGCGACGT GTGCTGTGAA GAACGGGCAG 
GCAGCGTGTG AATGCCTGCA GGCGTGCTCG AGCCTCTACG ATCCTGTGTG CGGCAGCGAC 
GGCGTCACAT ACGGCAGCGC GTGCGAGCTG GAGGCCACGG CCTGTACCCT CGGGCGGGAG 
ATCCAGGTGG CGCGCAAAGG ACCCTGTGAC CGCTGCGGGC AGTGCCGCTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG CTGCGTGTGC CCCTCTGAAT GCGTGGCTTT GGCCCAGCCC 
GTGTGTGGCT CCGACGGGCA CACGTACCCC AGCGAGTGCA TGCTGCACGT GCACGCCTGC 
ACACACCAGA TCAGCCTGCA CGTGGCCTCA GCTGGACCCT GTGAGACCTG TGGAGATGCC 
GTGTGTGCTT TTGGGGCTGT GTGCTCCGCA GGGCAGTGTG TGTGTCCCCG GTGTGAGCAC 
CCCCCGCCCG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACGGCAGTGC CTGCGAGCTA 
CGGGAAGCCG CCTGCCTCCA GCAGACACAG ATCGAGGAGG CCCGGGCAGG GCCGTGCGAG 
CAGGCCGAGT GCGGTTCCGG AGGCTCTGGC TCTGGGGAGG ACGGTGACTG TGAGCAGGAG 
CTGTGCCGGC AGCGCGGTGG CATCTGGGAC GAGGACTCGG AGGACGGGCC GTGTGTCTGT 
GACTTCAGCT GCCAGAGTGT CCCAGGCAGC CCGGTGTGCG GCTCAGATGG GGTCACCTAC 
AGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAGTCAC AGCGAGGGCT CTACGTAGCG 
GCCCAGGGAG CCTGCCGAGG CCCCACCTTC GCCCCGCTGC CGCCTGTGGC CCCCTTACAC 
TGTGCCCAGA CGCCCTACGG CTGCTGCCAG GACAATATCA CCGCAGCCCG GGGCGTGGGC 
CTGGCTGGCT GCCCCAGTGC CTGCCAGTGC AACCCCCATG GCTCTTACGG CGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCCTGCCGC CCAGGTGTGG GGGGCCTCAG GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTTCGAGGC ATCGTCACCG ATGGCCGGAG TGGCTGTACA 
CCCTGCAGCT GTGATCCCCA AGGCGCCGTG CGGGATGACT GTGAGCAGAT GACGGGGCTG 
TGCTCGTGTA AGCCCGGGGT GGCTGGACCC AAGTGTGGGC AGTGTCCAGA CGGCCGTGCC 
CTGGGCCCCG CGGGCTGTGA AGCTGACGCT TCTGCGCCTG CGACCTGTGC GGAGATGCGC 
TGTGAGTTCG GTGCGCGGTG CGTGGAGGAG TCTGGCTCAG CCCACTGTGT CTGCCCGATG 
CTCACCTGTC CAGAGGCCAA CGCTACCAAG GTCTGTGGGT CAGATGGAGT CACATACGGC 
AACGAGTGTC AGCTGAAGAC CATCGCCTGC CGCCAGGGCC TGCAAATCTC TATCCAGAGC 
CTGGGCCCGT GCCAGGAGGC TGTTGCTCCC AGCACTCACC CGACATCTGC CTCCGTGACT 
GTGACCACCC CAGGGCTCCT CCTGAGCCAG GCACTGCCGG CCCCCCCCGG CGCCCTCCCC 
CTGGCTCCCA GCAGTACCGC ACACAGCCAG ACCACCCCTC CGCCCTCATC ACGACCTCGG 
ACCACTGCCA GCGTCCCCAG GACCACCGTG TGGCCCGTGC TGACGGTGCC CCCCACGGCA 
CCCTCCCCTG CACCCAGCCT GGTGGCGTCC GCCTTTGGTG AATCTGGCAG CACTGATGGA 
AGCAGCGATG AGGAACTGAG CGGGGACCAG GAGGCCAGTG GGGGTGGCTC TGGGGGGCTC 
GAGCCCTTGG AGGGCAGCAG CGTGGCCACC CCTGGGCCAC CTGTCGAGAG GGCTTCCTGC 
TACAACTCCG CGTTGGGCTG CTGCTCTGAT GGGAAGACGC CCTCGCTGGA CGCAGAGGGC 
TCCAACTGCC CCGCCACCAA GGTGTTCCAG GGCGTCCTGG AGCTGGAGGG CGTCGAGGGC 
CAGGAGCTGT TCTACACGCC CGAGATGGCT GACCCCAAGT CAGAACTGTT CGGGGAGACA 
GCCAGGAGCA TTGAGAGCAC CCTGGACGAC CTCTTCCGGA ATTCAGACGT CAAGAAGGAT 
TTTCGGAGTG TCCGCTTGCG GGACCTGGGG CCCGGCAAAT CCGTCCGCGC CATTGTGGAT 
GTGCACTTTG ACCCCACCAC AGCCTTCAGG GCACCCGACG TGGCCCGGGC CCTGCTCCGG 
CAGATCCAGG TGTCCAGGCG CCGGTCCTTG GGGGTGAGGC GGCCGCTGCA GGAGCACGTG 
CGATTTATGG ACTTTGACTG GTTTCCTGCG TTTATCACGG GGGCCACGTC AGGAGCCATT 
GCTGCGGGAG CCACGGCCAG AGCCACCACT GCATCGCGCC TGCCGTCCTC TGCTGTGACC 
CCTCGGGCCC CGCACCCCAG TCACACAAGC CAGCCCGTTG CCAAGACCAC GGCAGCCCCC 
ACCACACGTC GGCCCCCCAC CACTGCCCCC AGCCGTGTGC CCGGACGTCG GCCCCCGGCC 
CCCCAGCAGC CTCCAAAGCC CTGTGACTCA CAGCCCTGCT TCCACGGGGG GACCTGCCAG 
GACTGGGCAT TGGGCGGGGG CTTCACCTGC AGCTGCCCGG CAGGCAGGGG AGGCGCCGTC 
TGTGAGAAGG TGCTTGGCGC CCCTGTGCCG GCCTTCGAGG GCCGCTCCTT CCTGGCCTTC 
CCCACTCTCC GCGCCTACCA CACGCTGCGC CTGGCACTGG AATTCCGGGC GCTGGAGCCT 
CAGGGGCTGC TGCTGTACAA TGGCAACGCC CGGGGCAAGG ACTTCCTGGC ATTGGCGCTG 
CTAGATGGCC GCGTGCAGCT CAGGTTTGAC ACAGGTTCGG GGCCGGCGGT GCTGACCAGT 
GCCGTGCCGG TAGAGCCGGG CCAGTGGCAC CGCCTGGAGC TGTCCCGGCA CTGGCGCCGG 
GGCACCCTCT CGGTGGATGG TGAGACCCCT GTTCTGGGCG AGAGTCCCAG TGGCACCGAC 
GGCCTCAACC TGGACACAGA CCTCTTTGTG GGCGGCGTAC CCGAGGACCA GGCTGCCGTG 
GCGCTGGAGC GGACCTTCGT GGGCGCCGGC CTGAGGGGGT GCATCCGTTT GCTGGACGTC 
AACAACCAGC GCCTGGAGCT TGGCATTGGG CCGGGGGCTG CCACCCGAGG CTCTGGCGTG 
GGCGAGTGCG GGGACCACCC CTGCCTGCCC AACCCCTGCC ATGGCGGGGC CCCATGCCAG 
AACCTGGAGG CTGGAAGGTT CCATTGCCAG TGCCCGCCCG GCCGCGTCGG ACCAACCTGT 
GCCGATGAGA AGAGCCCCTG CCAGCCCAAC CCCTGCCATG GGGCGGCGCC CTGCCGTGTG 
CTGCCCGAGG GTGGTGCTCA GTGCGAGTGC CCCCTGGGGC GTGAGGGCAC CTTCTGCCAG 
ACAGCCTCGG GGCAGGACGG CTCTGGGCCC TTCCTGGCTG ACTTCAACGG CTTCTCCCAC 
CTGGAGCTGA GAGGCCTGCA CACCTTTGCA CGGGACCTGG GGGAGAAGAT GGCGCTGGAG 
GTCGTGTTCC TGGCACGAGG CCCCAGCGGC CTCCTGCTCT ACAACGGGCA GAAGACGGAC 
GGCAAGGGGG ACTTCGTGTC GCTGGCACTG CGGGACCGCC GCCTGGAGTT CCGCTACGAC 
CTGGGCAAGG GGGCAGCGGT CATCAGGAGC AGGGAGCCAG TCACCCTGGG AGCCTGGACC 
AGGGTCTCAC TGGAGCGAAA CGGCCGCAAG GGTGCCCTGC GTGTGGGCGA CGGCCCCCGT 
GTGTTGGGGG AGTCCCCGAA ATCCCGCAAG GTTCCGCACA CCGTCCTCAA CCTGAAGGAG 
CCGCTCTACG TAGGGGGCGC TCCCGACTTC AGCAAGCTGG CCCGTGCTGC TGCCGTGTCC 
TCTGGCTTCG ACGGTGCCAT CCAGCTGGTC TCCCTCGGAG GCCGCCAGCT GCTGACCCCG 
GAGCACGTGC TGCGGCAGGT GGACGTCACG TCCTTTGCAG GTCACCCCTG CACCCGGGCC 
TCAGGCCACC CCTGCCTCAA TGGGGCCTCC TGCGTCCCGA GGGAGGCTGC CTATGTGTGC 
CTGTGTCCCG GGGGATTCTC AGGACCGCAC TGCGAGAAGG GGCTGGTGGA GAAGTCAGCG 
GGGGACGTGG ATACCTTGGC CTTTGACGGG CGGACCTTTG TCGAGTACCT CAACGCTGTG 
ACCGAGAGCG AGAAGGCACT GCAGAGCAAC CACTTTGAAC TGAGCCTGCG CACTGAGGCC 
ACGCAGGGGC TGGTGCTCTG GAGTGGCAAG GCCACGGAGC GGGCAGACTA TGTGGCACTG 
GCCATTGTGG ACGGGCACCT GCAACTGAGC TACAACCTGG GCTCCCAGCC CGTGGTGCTG 
CGTTCCACCG TGCCCGTCAA CACCAACCGC TGGTTGCGGG TCGTGGCACA TAGGGAGCAG 
AGGGAAGGTT CCCTGCAGGT GGGCAATGAG GCCCCTGTGA CCGGCTCCTC CCCGCTGGGC 
GCCACGCAGC TGGACACTGA TGGAGCCCTG TGGCTTGGGG GCCTGCCGGA GCTGCCCGTG 
GGCCCAGCAC TGCCCAAGGC CTACGGCACA GGCTTTGTGG GCTGCTTGCG GGACGTGGTG 
GTGGGCCGGC ACCCGCTGCA CCTGCTGGAG GACGCCGTCA CCAAGCCAGA GCTGCGGCCC 
TGCCCCACCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( NM_001364727.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001351656.1 Homo sapiens agrin (AGRN), transcript variant 3, mRNA.

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CloneID	OHu107768
Clone ID Related Accession (Same CDS sequence)	NM_001364727.2 , NM_001364727.1
Accession Version	NM_001364727.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	agrin isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645608.30. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGCCTCCGC TGCCGCTGGC GCGGGACACC CGGCAGCCGC CTGGCGCCTC CCTGCTGGTG 
CGAGGCTTCA TGGTGCCCTG CAACGCCTGC CTGATCCTGC TGGCCACCGC CACGCTCGGC 
TTCGCGGTGC TGCTGTTCCT CAACAACTAT AAACCCGGGA CCCACTTCAC TCCAGTGCCT 
CCGACGCCTC CTGATGCGTG CCGGGGAATG CTGTGCGGCT TCGGCGCCGT GTGCGAGCCC 
AACGCGGAGG GGCCGGGCCG GGCGTCCTGC GTCTGCAAGA AGAGCCCGTG CCCCAGCGTG 
GTGGCGCCTG TGTGTGGGTC GGACGCCTCC ACCTACAGCA ACGAATGCGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTGCTCAGCC GCGGGCCGTG CGGCTCGCGG 
GACCCCTGCT CCAACGTGAC CTGCAGCTTC GGCAGCACCT GTGCGCGCTC GGCCGACGGG 
CTGACGGCCT CGTGCCTGTG CCCCGCGACC TGCCGTGGCG CCCCCGAGGG GACCGTCTGC 
GGCAGCGACG GCGCCGACTA CCCCGGCGAG TGCCAGCTCC TGCGCCGCGC CTGCGCCCGC 
CAGGAGAATG TCTTCAAGAA GTTCGACGGC CCTTGTGACC CCTGTCAGGG CGCCCTCCCT 
GACCCGAGCC GCAGCTGCCG TGTGAACCCG CGCACGCGGC GCCCTGAGAT GCTCCTACGG 
CCCGAGAGCT GCCCTGCCCG GCAGGCGCCA GTGTGTGGGG ACGACGGAGT CACCTACGAA 
AACGACTGTG TCATGGGCCG ATCGGGGGCC GCCCGGGGTC TCCTCCTGCA GAAAGTGCGC 
TCCGGCCAGT GCCAGGGTCG AGACCAGTGC CCGGAGCCCT GCCGGTTCAA TGCCGTGTGC 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGCGACCGCG TCACCTGTGA CGGGGCCTAC 
AGGCCCGTGT GTGCCCAGGA CGGGCGCACG TATGACAGTG ATTGCTGGCG GCAGCAGGCT 
GAGTGCCGGC AGCAGCGTGC CATCCCCAGC AAGCACCAGG GCCCGTGTGA CCAGGCCCCG 
TCCCCATGCC TCGGGGTGCA GTGTGCATTT GGGGCGACGT GTGCTGTGAA GAACGGGCAG 
GCAGCGTGTG AATGCCTGCA GGCGTGCTCG AGCCTCTACG ATCCTGTGTG CGGCAGCGAC 
GGCGTCACAT ACGGCAGCGC GTGCGAGCTG GAGGCCACGG CCTGTACCCT CGGGCGGGAG 
ATCCAGGTGG CGCGCAAAGG ACCCTGTGAC CGCTGCGGGC AGTGCCGCTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG CTGCGTGTGC CCCTCTGAAT GCGTGGCTTT GGCCCAGCCC 
GTGTGTGGCT CCGACGGGCA CACGTACCCC AGCGAGTGCA TGCTGCACGT GCACGCCTGC 
ACACACCAGA TCAGCCTGCA CGTGGCCTCA GCTGGACCCT GTGAGACCTG TGGAGATGCC 
GTGTGTGCTT TTGGGGCTGT GTGCTCCGCA GGGCAGTGTG TGTGTCCCCG GTGTGAGCAC 
CCCCCGCCCG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACGGCAGTGC CTGCGAGCTA 
CGGGAAGCCG CCTGCCTCCA GCAGACACAG ATCGAGGAGG CCCGGGCAGG GCCGTGCGAG 
CAGGCCGAGT GCGGTTCCGG AGGCTCTGGC TCTGGGGAGG ACGGTGACTG TGAGCAGGAG 
CTGTGCCGGC AGCGCGGTGG CATCTGGGAC GAGGACTCGG AGGACGGGCC GTGTGTCTGT 
GACTTCAGCT GCCAGAGTGT CCCAGGCAGC CCGGTGTGCG GCTCAGATGG GGTCACCTAC 
AGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAGTCAC AGCGAGGGCT CTACGTAGCG 
GCCCAGGGAG CCTGCCGAGG CCCCACCTTC GCCCCGCTGC CGCCTGTGGC CCCCTTACAC 
TGTGCCCAGA CGCCCTACGG CTGCTGCCAG GACAATATCA CCGCAGCCCG GGGCGTGGGC 
CTGGCTGGCT GCCCCAGTGC CTGCCAGTGC AACCCCCATG GCTCTTACGG CGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCCTGCCGC CCAGGTGTGG GGGGCCTCAG GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTTCGAGGC ATCGTCACCG ATGGCCGGAG TGGCTGTACA 
CCCTGCAGCT GTGATCCCCA AGGCGCCGTG CGGGATGACT GTGAGCAGAT GACGGGGCTG 
TGCTCGTGTA AGCCCGGGGT GGCTGGACCC AAGTGTGGGC AGTGTCCAGA CGGCCGTGCC 
CTGGGCCCCG CGGGCTGTGA AGCTGACGCT TCTGCGCCTG CGACCTGTGC GGAGATGCGC 
TGTGAGTTCG GTGCGCGGTG CGTGGAGGAG TCTGGCTCAG CCCACTGTGT CTGCCCGATG 
CTCACCTGTC CAGAGGCCAA CGCTACCAAG GTCTGTGGGT CAGATGGAGT CACATACGGC 
AACGAGTGTC AGCTGAAGAC CATCGCCTGC CGCCAGGGCC TGCAAATCTC TATCCAGAGC 
CTGGGCCCGT GCCAGGAGGC TGTTGCTCCC AGCACTCACC CGACATCTGC CTCCGTGACT 
GTGACCACCC CAGGGCTCCT CCTGAGCCAG GCACTGCCGG CCCCCCCCGG CGCCCTCCCC 
CTGGCTCCCA GCAGTACCGC ACACAGCCAG ACCACCCCTC CGCCCTCATC ACGACCTCGG 
ACCACTGCCA GCGTCCCCAG GACCACCGTG TGGCCCGTGC TGACGGTGCC CCCCACGGCA 
CCCTCCCCTG CACCCAGCCT GGTGGCGTCC GCCTTTGGTG AATCTGGCAG CACTGATGGA 
AGCAGCGATG AGGAACTGAG CGGGGACCAG GAGGCCAGTG GGGGTGGCTC TGGGGGGCTC 
GAGCCCTTGG AGGGCAGCAG CGTGGCCACC CCTGGGCCAC CTGTCGAGAG GGCTTCCTGC 
TACAACTCCG CGTTGGGCTG CTGCTCTGAT GGGAAGACGC CCTCGCTGGA CGCAGAGGGC 
TCCAACTGCC CCGCCACCAA GGTGTTCCAG GGCGTCCTGG AGCTGGAGGG CGTCGAGGGC 
CAGGAGCTGT TCTACACGCC CGAGATGGCT GACCCCAAGT CAGAACTGTT CGGGGAGACA 
GCCAGGAGCA TTGAGAGCAC CCTGGACGAC CTCTTCCGGA ATTCAGACGT CAAGAAGGAT 
TTTCGGAGTG TCCGCTTGCG GGACCTGGGG CCCGGCAAAT CCGTCCGCGC CATTGTGGAT 
GTGCACTTTG ACCCCACCAC AGCCTTCAGG GCACCCGACG TGGCCCGGGC CCTGCTCCGG 
CAGATCCAGG TGTCCAGGCG CCGGTCCTTG GGGGTGAGGC GGCCGCTGCA GGAGCACGTG 
CGATTTATGG ACTTTGACTG GTTTCCTGCG TTTATCACGG GGGCCACGTC AGGAGCCATT 
GCTGCGGGAG CCACGGCCAG AGCCACCACT GCATCGCGCC TGCCGTCCTC TGCTGTGACC 
CCTCGGGCCC CGCACCCCAG TCACACAAGC CAGCCCGTTG CCAAGACCAC GGCAGCCCCC 
ACCACACGTC GGCCCCCCAC CACTGCCCCC AGCCGTGTGC CCGGACGTCG GCCCCCGGCC 
CCCCAGCAGC CTCCAAAGCC CTGTGACTCA CAGCCCTGCT TCCACGGGGG GACCTGCCAG 
GACTGGGCAT TGGGCGGGGG CTTCACCTGC AGCTGCCCGG CAGGCAGGGG AGGCGCCGTC 
TGTGAGAAGG TGCTTGGCGC CCCTGTGCCG GCCTTCGAGG GCCGCTCCTT CCTGGCCTTC 
CCCACTCTCC GCGCCTACCA CACGCTGCGC CTGGCACTGG AATTCCGGGC GCTGGAGCCT 
CAGGGGCTGC TGCTGTACAA TGGCAACGCC CGGGGCAAGG ACTTCCTGGC ATTGGCGCTG 
CTAGATGGCC GCGTGCAGCT CAGGTTTGAC ACAGGTTCGG GGCCGGCGGT GCTGACCAGT 
GCCGTGCCGG TAGAGCCGGG CCAGTGGCAC CGCCTGGAGC TGTCCCGGCA CTGGCGCCGG 
GGCACCCTCT CGGTGGATGG TGAGACCCCT GTTCTGGGCG AGAGTCCCAG TGGCACCGAC 
GGCCTCAACC TGGACACAGA CCTCTTTGTG GGCGGCGTAC CCGAGGACCA GGCTGCCGTG 
GCGCTGGAGC GGACCTTCGT GGGCGCCGGC CTGAGGGGGT GCATCCGTTT GCTGGACGTC 
AACAACCAGC GCCTGGAGCT TGGCATTGGG CCGGGGGCTG CCACCCGAGG CTCTGGCGTG 
GGCGAGTGCG GGGACCACCC CTGCCTGCCC AACCCCTGCC ATGGCGGGGC CCCATGCCAG 
AACCTGGAGG CTGGAAGGTT CCATTGCCAG TGCCCGCCCG GCCGCGTCGG ACCAACCTGT 
GCCGATGAGA AGAGCCCCTG CCAGCCCAAC CCCTGCCATG GGGCGGCGCC CTGCCGTGTG 
CTGCCCGAGG GTGGTGCTCA GTGCGAGTGC CCCCTGGGGC GTGAGGGCAC CTTCTGCCAG 
ACAGCCTCGG GGCAGGACGG CTCTGGGCCC TTCCTGGCTG ACTTCAACGG CTTCTCCCAC 
CTGGAGCTGA GAGGCCTGCA CACCTTTGCA CGGGACCTGG GGGAGAAGAT GGCGCTGGAG 
GTCGTGTTCC TGGCACGAGG CCCCAGCGGC CTCCTGCTCT ACAACGGGCA GAAGACGGAC 
GGCAAGGGGG ACTTCGTGTC GCTGGCACTG CGGGACCGCC GCCTGGAGTT CCGCTACGAC 
CTGGGCAAGG GGGCAGCGGT CATCAGGAGC AGGGAGCCAG TCACCCTGGG AGCCTGGACC 
AGGGTCTCAC TGGAGCGAAA CGGCCGCAAG GGTGCCCTGC GTGTGGGCGA CGGCCCCCGT 
GTGTTGGGGG AGTCCCCGAA ATCCCGCAAG GTTCCGCACA CCGTCCTCAA CCTGAAGGAG 
CCGCTCTACG TAGGGGGCGC TCCCGACTTC AGCAAGCTGG CCCGTGCTGC TGCCGTGTCC 
TCTGGCTTCG ACGGTGCCAT CCAGCTGGTC TCCCTCGGAG GCCGCCAGCT GCTGACCCCG 
GAGCACGTGC TGCGGCAGGT GGACGTCACG TCCTTTGCAG GTCACCCCTG CACCCGGGCC 
TCAGGCCACC CCTGCCTCAA TGGGGCCTCC TGCGTCCCGA GGGAGGCTGC CTATGTGTGC 
CTGTGTCCCG GGGGATTCTC AGGACCGCAC TGCGAGAAGG GGCTGGTGGA GAAGTCAGCG 
GGGGACGTGG ATACCTTGGC CTTTGACGGG CGGACCTTTG TCGAGTACCT CAACGCTGTG 
ACCGAGAGCG AGAAGGCACT GCAGAGCAAC CACTTTGAAC TGAGCCTGCG CACTGAGGCC 
ACGCAGGGGC TGGTGCTCTG GAGTGGCAAG GCCACGGAGC GGGCAGACTA TGTGGCACTG 
GCCATTGTGG ACGGGCACCT GCAACTGAGC TACAACCTGG GCTCCCAGCC CGTGGTGCTG 
CGTTCCACCG TGCCCGTCAA CACCAACCGC TGGTTGCGGG TCGTGGCACA TAGGGAGCAG 
AGGGAAGGTT CCCTGCAGGT GGGCAATGAG GCCCCTGTGA CCGGCTCCTC CCCGCTGGGC 
GCCACGCAGC TGGACACTGA TGGAGCCCTG TGGCTTGGGG GCCTGCCGGA GCTGCCCGTG 
GGCCCAGCAC TGCCCAAGGC CTACGGCACA GGCTTTGTGG GCTGCTTGCG GGACGTGGTG 
GTGGGCCGGC ACCCGCTGCA CCTGCTGGAG GACGCCGTCA CCAAGCCAGA GCTGCGGCCC 
TGCCCCACCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351656.1
CDS	564..6398
Translation	MPPLPLARDTRQPPGASLLVRGFMVPCNACLILLATATLGFAVLLFLNNYKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	NM_001364727.1
Organism	Homo sapiens(human)
Definition	Homo sapiens agrin (AGRN), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364727.1

ORF Insert Sequence:

ATGCCTCCGC TGCCGCTGGC GCGGGACACC CGGCAGCCGC CTGGCGCCTC CCTGCTGGTG 
CGAGGCTTCA TGGTGCCCTG CAACGCCTGC CTGATCCTGC TGGCCACCGC CACGCTCGGC 
TTCGCGGTGC TGCTGTTCCT CAACAACTAT AAACCCGGGA CCCACTTCAC TCCAGTGCCT 
CCGACGCCTC CTGATGCGTG CCGGGGAATG CTGTGCGGCT TCGGCGCCGT GTGCGAGCCC 
AACGCGGAGG GGCCGGGCCG GGCGTCCTGC GTCTGCAAGA AGAGCCCGTG CCCCAGCGTG 
GTGGCGCCTG TGTGTGGGTC GGACGCCTCC ACCTACAGCA ACGAATGCGA GCTGCAGCGG 
GCGCAGTGCA GCCAGCAGCG CCGCATCCGC CTGCTCAGCC GCGGGCCGTG CGGCTCGCGG 
GACCCCTGCT CCAACGTGAC CTGCAGCTTC GGCAGCACCT GTGCGCGCTC GGCCGACGGG 
CTGACGGCCT CGTGCCTGTG CCCCGCGACC TGCCGTGGCG CCCCCGAGGG GACCGTCTGC 
GGCAGCGACG GCGCCGACTA CCCCGGCGAG TGCCAGCTCC TGCGCCGCGC CTGCGCCCGC 
CAGGAGAATG TCTTCAAGAA GTTCGACGGC CCTTGTGACC CCTGTCAGGG CGCCCTCCCT 
GACCCGAGCC GCAGCTGCCG TGTGAACCCG CGCACGCGGC GCCCTGAGAT GCTCCTACGG 
CCCGAGAGCT GCCCTGCCCG GCAGGCGCCA GTGTGTGGGG ACGACGGAGT CACCTACGAA 
AACGACTGTG TCATGGGCCG ATCGGGGGCC GCCCGGGGTC TCCTCCTGCA GAAAGTGCGC 
TCCGGCCAGT GCCAGGGTCG AGACCAGTGC CCGGAGCCCT GCCGGTTCAA TGCCGTGTGC 
CTGTCCCGCC GTGGCCGTCC CCGCTGCTCC TGCGACCGCG TCACCTGTGA CGGGGCCTAC 
AGGCCCGTGT GTGCCCAGGA CGGGCGCACG TATGACAGTG ATTGCTGGCG GCAGCAGGCT 
GAGTGCCGGC AGCAGCGTGC CATCCCCAGC AAGCACCAGG GCCCGTGTGA CCAGGCCCCG 
TCCCCATGCC TCGGGGTGCA GTGTGCATTT GGGGCGACGT GTGCTGTGAA GAACGGGCAG 
GCAGCGTGTG AATGCCTGCA GGCGTGCTCG AGCCTCTACG ATCCTGTGTG CGGCAGCGAC 
GGCGTCACAT ACGGCAGCGC GTGCGAGCTG GAGGCCACGG CCTGTACCCT CGGGCGGGAG 
ATCCAGGTGG CGCGCAAAGG ACCCTGTGAC CGCTGCGGGC AGTGCCGCTT TGGAGCCCTG 
TGCGAGGCCG AGACCGGGCG CTGCGTGTGC CCCTCTGAAT GCGTGGCTTT GGCCCAGCCC 
GTGTGTGGCT CCGACGGGCA CACGTACCCC AGCGAGTGCA TGCTGCACGT GCACGCCTGC 
ACACACCAGA TCAGCCTGCA CGTGGCCTCA GCTGGACCCT GTGAGACCTG TGGAGATGCC 
GTGTGTGCTT TTGGGGCTGT GTGCTCCGCA GGGCAGTGTG TGTGTCCCCG GTGTGAGCAC 
CCCCCGCCCG GCCCCGTGTG TGGCAGCGAC GGTGTCACCT ACGGCAGTGC CTGCGAGCTA 
CGGGAAGCCG CCTGCCTCCA GCAGACACAG ATCGAGGAGG CCCGGGCAGG GCCGTGCGAG 
CAGGCCGAGT GCGGTTCCGG AGGCTCTGGC TCTGGGGAGG ACGGTGACTG TGAGCAGGAG 
CTGTGCCGGC AGCGCGGTGG CATCTGGGAC GAGGACTCGG AGGACGGGCC GTGTGTCTGT 
GACTTCAGCT GCCAGAGTGT CCCAGGCAGC CCGGTGTGCG GCTCAGATGG GGTCACCTAC 
AGCACCGAGT GTGAGCTGAA GAAGGCCAGG TGTGAGTCAC AGCGAGGGCT CTACGTAGCG 
GCCCAGGGAG CCTGCCGAGG CCCCACCTTC GCCCCGCTGC CGCCTGTGGC CCCCTTACAC 
TGTGCCCAGA CGCCCTACGG CTGCTGCCAG GACAATATCA CCGCAGCCCG GGGCGTGGGC 
CTGGCTGGCT GCCCCAGTGC CTGCCAGTGC AACCCCCATG GCTCTTACGG CGGCACCTGT 
GACCCAGCCA CAGGCCAGTG CTCCTGCCGC CCAGGTGTGG GGGGCCTCAG GTGTGACCGC 
TGTGAGCCTG GCTTCTGGAA CTTTCGAGGC ATCGTCACCG ATGGCCGGAG TGGCTGTACA 
CCCTGCAGCT GTGATCCCCA AGGCGCCGTG CGGGATGACT GTGAGCAGAT GACGGGGCTG 
TGCTCGTGTA AGCCCGGGGT GGCTGGACCC AAGTGTGGGC AGTGTCCAGA CGGCCGTGCC 
CTGGGCCCCG CGGGCTGTGA AGCTGACGCT TCTGCGCCTG CGACCTGTGC GGAGATGCGC 
TGTGAGTTCG GTGCGCGGTG CGTGGAGGAG TCTGGCTCAG CCCACTGTGT CTGCCCGATG 
CTCACCTGTC CAGAGGCCAA CGCTACCAAG GTCTGTGGGT CAGATGGAGT CACATACGGC 
AACGAGTGTC AGCTGAAGAC CATCGCCTGC CGCCAGGGCC TGCAAATCTC TATCCAGAGC 
CTGGGCCCGT GCCAGGAGGC TGTTGCTCCC AGCACTCACC CGACATCTGC CTCCGTGACT 
GTGACCACCC CAGGGCTCCT CCTGAGCCAG GCACTGCCGG CCCCCCCCGG CGCCCTCCCC 
CTGGCTCCCA GCAGTACCGC ACACAGCCAG ACCACCCCTC CGCCCTCATC ACGACCTCGG 
ACCACTGCCA GCGTCCCCAG GACCACCGTG TGGCCCGTGC TGACGGTGCC CCCCACGGCA 
CCCTCCCCTG CACCCAGCCT GGTGGCGTCC GCCTTTGGTG AATCTGGCAG CACTGATGGA 
AGCAGCGATG AGGAACTGAG CGGGGACCAG GAGGCCAGTG GGGGTGGCTC TGGGGGGCTC 
GAGCCCTTGG AGGGCAGCAG CGTGGCCACC CCTGGGCCAC CTGTCGAGAG GGCTTCCTGC 
TACAACTCCG CGTTGGGCTG CTGCTCTGAT GGGAAGACGC CCTCGCTGGA CGCAGAGGGC 
TCCAACTGCC CCGCCACCAA GGTGTTCCAG GGCGTCCTGG AGCTGGAGGG CGTCGAGGGC 
CAGGAGCTGT TCTACACGCC CGAGATGGCT GACCCCAAGT CAGAACTGTT CGGGGAGACA 
GCCAGGAGCA TTGAGAGCAC CCTGGACGAC CTCTTCCGGA ATTCAGACGT CAAGAAGGAT 
TTTCGGAGTG TCCGCTTGCG GGACCTGGGG CCCGGCAAAT CCGTCCGCGC CATTGTGGAT 
GTGCACTTTG ACCCCACCAC AGCCTTCAGG GCACCCGACG TGGCCCGGGC CCTGCTCCGG 
CAGATCCAGG TGTCCAGGCG CCGGTCCTTG GGGGTGAGGC GGCCGCTGCA GGAGCACGTG 
CGATTTATGG ACTTTGACTG GTTTCCTGCG TTTATCACGG GGGCCACGTC AGGAGCCATT 
GCTGCGGGAG CCACGGCCAG AGCCACCACT GCATCGCGCC TGCCGTCCTC TGCTGTGACC 
CCTCGGGCCC CGCACCCCAG TCACACAAGC CAGCCCGTTG CCAAGACCAC GGCAGCCCCC 
ACCACACGTC GGCCCCCCAC CACTGCCCCC AGCCGTGTGC CCGGACGTCG GCCCCCGGCC 
CCCCAGCAGC CTCCAAAGCC CTGTGACTCA CAGCCCTGCT TCCACGGGGG GACCTGCCAG 
GACTGGGCAT TGGGCGGGGG CTTCACCTGC AGCTGCCCGG CAGGCAGGGG AGGCGCCGTC 
TGTGAGAAGG TGCTTGGCGC CCCTGTGCCG GCCTTCGAGG GCCGCTCCTT CCTGGCCTTC 
CCCACTCTCC GCGCCTACCA CACGCTGCGC CTGGCACTGG AATTCCGGGC GCTGGAGCCT 
CAGGGGCTGC TGCTGTACAA TGGCAACGCC CGGGGCAAGG ACTTCCTGGC ATTGGCGCTG 
CTAGATGGCC GCGTGCAGCT CAGGTTTGAC ACAGGTTCGG GGCCGGCGGT GCTGACCAGT 
GCCGTGCCGG TAGAGCCGGG CCAGTGGCAC CGCCTGGAGC TGTCCCGGCA CTGGCGCCGG 
GGCACCCTCT CGGTGGATGG TGAGACCCCT GTTCTGGGCG AGAGTCCCAG TGGCACCGAC 
GGCCTCAACC TGGACACAGA CCTCTTTGTG GGCGGCGTAC CCGAGGACCA GGCTGCCGTG 
GCGCTGGAGC GGACCTTCGT GGGCGCCGGC CTGAGGGGGT GCATCCGTTT GCTGGACGTC 
AACAACCAGC GCCTGGAGCT TGGCATTGGG CCGGGGGCTG CCACCCGAGG CTCTGGCGTG 
GGCGAGTGCG GGGACCACCC CTGCCTGCCC AACCCCTGCC ATGGCGGGGC CCCATGCCAG 
AACCTGGAGG CTGGAAGGTT CCATTGCCAG TGCCCGCCCG GCCGCGTCGG ACCAACCTGT 
GCCGATGAGA AGAGCCCCTG CCAGCCCAAC CCCTGCCATG GGGCGGCGCC CTGCCGTGTG 
CTGCCCGAGG GTGGTGCTCA GTGCGAGTGC CCCCTGGGGC GTGAGGGCAC CTTCTGCCAG 
ACAGCCTCGG GGCAGGACGG CTCTGGGCCC TTCCTGGCTG ACTTCAACGG CTTCTCCCAC 
CTGGAGCTGA GAGGCCTGCA CACCTTTGCA CGGGACCTGG GGGAGAAGAT GGCGCTGGAG 
GTCGTGTTCC TGGCACGAGG CCCCAGCGGC CTCCTGCTCT ACAACGGGCA GAAGACGGAC 
GGCAAGGGGG ACTTCGTGTC GCTGGCACTG CGGGACCGCC GCCTGGAGTT CCGCTACGAC 
CTGGGCAAGG GGGCAGCGGT CATCAGGAGC AGGGAGCCAG TCACCCTGGG AGCCTGGACC 
AGGGTCTCAC TGGAGCGAAA CGGCCGCAAG GGTGCCCTGC GTGTGGGCGA CGGCCCCCGT 
GTGTTGGGGG AGTCCCCGAA ATCCCGCAAG GTTCCGCACA CCGTCCTCAA CCTGAAGGAG 
CCGCTCTACG TAGGGGGCGC TCCCGACTTC AGCAAGCTGG CCCGTGCTGC TGCCGTGTCC 
TCTGGCTTCG ACGGTGCCAT CCAGCTGGTC TCCCTCGGAG GCCGCCAGCT GCTGACCCCG 
GAGCACGTGC TGCGGCAGGT GGACGTCACG TCCTTTGCAG GTCACCCCTG CACCCGGGCC 
TCAGGCCACC CCTGCCTCAA TGGGGCCTCC TGCGTCCCGA GGGAGGCTGC CTATGTGTGC 
CTGTGTCCCG GGGGATTCTC AGGACCGCAC TGCGAGAAGG GGCTGGTGGA GAAGTCAGCG 
GGGGACGTGG ATACCTTGGC CTTTGACGGG CGGACCTTTG TCGAGTACCT CAACGCTGTG 
ACCGAGAGCG AGAAGGCACT GCAGAGCAAC CACTTTGAAC TGAGCCTGCG CACTGAGGCC 
ACGCAGGGGC TGGTGCTCTG GAGTGGCAAG GCCACGGAGC GGGCAGACTA TGTGGCACTG 
GCCATTGTGG ACGGGCACCT GCAACTGAGC TACAACCTGG GCTCCCAGCC CGTGGTGCTG 
CGTTCCACCG TGCCCGTCAA CACCAACCGC TGGTTGCGGG TCGTGGCACA TAGGGAGCAG 
AGGGAAGGTT CCCTGCAGGT GGGCAATGAG GCCCCTGTGA CCGGCTCCTC CCCGCTGGGC 
GCCACGCAGC TGGACACTGA TGGAGCCCTG TGGCTTGGGG GCCTGCCGGA GCTGCCCGTG 
GGCCCAGCAC TGCCCAAGGC CTACGGCACA GGCTTTGTGG GCTGCTTGCG GGACGTGGTG 
GTGGGCCGGC ACCCGCTGCA CCTGCTGGAG GACGCCGTCA CCAAGCCAGA GCTGCGGCCC 
TGCCCCACCC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( NM_198576.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_940978.2 Homo sapiens agrin (AGRN), transcript variant 2, mRNA.

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CloneID	OHu17750
Clone ID Related Accession (Same CDS sequence)	NM_198576.4 , NM_198576.3
Accession Version	NM_198576.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	agrin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645608.30, AB191264.1, CN314674.1, CN314678.1, AW119098.1, BC007649.1 and CK000983.1. On Nov 22, 2018 this sequence version replaced NM_198576.3. Transcript Variant: This variant (2) lacks alternate in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB191264.1, AF016903.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000379370.7/ ENSP00000368678.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC 
GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG 
GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG 
CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG 
GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC 
CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA 
CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG 
ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC 
TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC 
GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC 
CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA 
TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG 
CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG 
CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC 
GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC 
CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT 
CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT 
GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC 
GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC 
CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG 
TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC 
TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC 
TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG 
TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT 
GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT 
GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT 
ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC 
CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG 
GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG 
CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG 
ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT 
CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC 
AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG 
GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT 
GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC 
GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA 
GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA 
GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT 
GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA 
GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT 
TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC 
CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC 
CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG 
CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT 
CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC 
TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC 
TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT 
GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA 
ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA 
TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC 
CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC 
TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG 
GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT 
GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT 
GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC 
GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG 
CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG 
GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA 
CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA 
GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC 
CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC 
CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG 
CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC 
ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG 
TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG 
ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA 
CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC 
GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC 
AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC 
TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC 
CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC 
CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG 
GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC 
CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT 
CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG 
GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC 
CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC 
CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC 
GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC 
GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG 
GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG 
GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC 
AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG 
AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC 
GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG 
GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC 
CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG 
GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGGTTCCGC ACACCGTCCT CAACCTGAAG 
GAGCCGCTCT ACGTAGGGGG CGCTCCCGAC TTCAGCAAGC TGGCCCGTGC TGCTGCCGTG 
TCCTCTGGCT TCGACGGTGC CATCCAGCTG GTCTCCCTCG GAGGCCGCCA GCTGCTGACC 
CCGGAGCACG TGCTGCGGCA GGTGGACGTC ACGTCCTTTG CAGGTCACCC CTGCACCCGG 
GCCTCAGGCC ACCCCTGCCT CAATGGGGCC TCCTGCGTCC CGAGGGAGGC TGCCTATGTG 
TGCCTGTGTC CCGGGGGATT CTCAGGACCG CACTGCGAGA AGGGGCTGGT GGAGAAGTCA 
GCGGGGGACG TGGATACCTT GGCCTTTGAC GGGCGGACCT TTGTCGAGTA CCTCAACGCT 
GTGACCGAGA GCGAGAAGGC ACTGCAGAGC AACCACTTTG AACTGAGCCT GCGCACTGAG 
GCCACGCAGG GGCTGGTGCT CTGGAGTGGC AAGGCCACGG AGCGGGCAGA CTATGTGGCA 
CTGGCCATTG TGGACGGGCA CCTGCAACTG AGCTACAACC TGGGCTCCCA GCCCGTGGTG 
CTGCGTTCCA CCGTGCCCGT CAACACCAAC CGCTGGTTGC GGGTCGTGGC ACATAGGGAG 
CAGAGGGAAG GTTCCCTGCA GGTGGGCAAT GAGGCCCCTG TGACCGGCTC CTCCCCGCTG 
GGCGCCACGC AGCTGGACAC TGATGGAGCC CTGTGGCTTG GGGGCCTGCC GGAGCTGCCC 
GTGGGCCCAG CACTGCCCAA GGCCTACGGC ACAGGCTTTG TGGGCTGCTT GCGGGACGTG 
GTGGTGGGCC GGCACCCGCT GCACCTGCTG GAGGACGCCG TCACCAAGCC AGAGCTGCGG 
CCCTGCCCCA CCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_940978.2
CDS	54..6191
Translation	MAGRSHPGPLRPLLPLLVVAACVLPGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	NM_198576.4
Organism	Homo sapiens(human)
Definition	Homo sapiens agrin (AGRN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198576.4

ORF Insert Sequence:

ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC 
GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG 
GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG 
CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG 
GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC 
CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA 
CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG 
ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC 
TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC 
GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC 
CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA 
TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG 
CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG 
CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC 
GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC 
CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT 
CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT 
GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC 
GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC 
CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG 
TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC 
TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC 
TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG 
TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT 
GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT 
GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT 
ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC 
CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG 
GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG 
CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG 
ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT 
CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC 
AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG 
GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT 
GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC 
GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA 
GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA 
GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT 
GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA 
GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT 
TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC 
CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC 
CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG 
CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT 
CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC 
TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC 
TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT 
GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA 
ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA 
TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC 
CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC 
TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG 
GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT 
GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT 
GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC 
GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG 
CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG 
GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA 
CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA 
GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC 
CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC 
CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG 
CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC 
ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG 
TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG 
ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA 
CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC 
GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC 
AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC 
TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC 
CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC 
CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG 
GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC 
CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT 
CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG 
GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC 
CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC 
CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC 
GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC 
GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG 
GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG 
GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC 
AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG 
AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC 
GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG 
GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC 
CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG 
GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGGTTCCGC ACACCGTCCT CAACCTGAAG 
GAGCCGCTCT ACGTAGGGGG CGCTCCCGAC TTCAGCAAGC TGGCCCGTGC TGCTGCCGTG 
TCCTCTGGCT TCGACGGTGC CATCCAGCTG GTCTCCCTCG GAGGCCGCCA GCTGCTGACC 
CCGGAGCACG TGCTGCGGCA GGTGGACGTC ACGTCCTTTG CAGGTCACCC CTGCACCCGG 
GCCTCAGGCC ACCCCTGCCT CAATGGGGCC TCCTGCGTCC CGAGGGAGGC TGCCTATGTG 
TGCCTGTGTC CCGGGGGATT CTCAGGACCG CACTGCGAGA AGGGGCTGGT GGAGAAGTCA 
GCGGGGGACG TGGATACCTT GGCCTTTGAC GGGCGGACCT TTGTCGAGTA CCTCAACGCT 
GTGACCGAGA GCGAGAAGGC ACTGCAGAGC AACCACTTTG AACTGAGCCT GCGCACTGAG 
GCCACGCAGG GGCTGGTGCT CTGGAGTGGC AAGGCCACGG AGCGGGCAGA CTATGTGGCA 
CTGGCCATTG TGGACGGGCA CCTGCAACTG AGCTACAACC TGGGCTCCCA GCCCGTGGTG 
CTGCGTTCCA CCGTGCCCGT CAACACCAAC CGCTGGTTGC GGGTCGTGGC ACATAGGGAG 
CAGAGGGAAG GTTCCCTGCA GGTGGGCAAT GAGGCCCCTG TGACCGGCTC CTCCCCGCTG 
GGCGCCACGC AGCTGGACAC TGATGGAGCC CTGTGGCTTG GGGGCCTGCC GGAGCTGCCC 
GTGGGCCCAG CACTGCCCAA GGCCTACGGC ACAGGCTTTG TGGGCTGCTT GCGGGACGTG 
GTGGTGGGCC GGCACCCGCT GCACCTGCTG GAGGACGCCG TCACCAAGCC AGAGCTGCGG 
CCCTGCCCCA CCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( NM_198576.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_940978.2 Homo sapiens agrin (AGRN), transcript variant 2, mRNA.

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CloneID	OHu17750
Clone ID Related Accession (Same CDS sequence)	NM_198576.4 , NM_198576.3
Accession Version	NM_198576.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	agrin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB191264.1, CN314674.1, CN314678.1, AW119098.1, BC007649.1, AL645608.30 and CK000983.1. On Aug 24, 2011 this sequence version replaced NM_198576.2. Transcript Variant: This variant (2) lacks alternate in-frame exons in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB191264.1, AF016903.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC 
GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG 
GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG 
CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG 
GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC 
CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA 
CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG 
ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC 
TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC 
GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC 
CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA 
TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG 
CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG 
CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC 
GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC 
CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT 
CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT 
GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC 
GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC 
CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG 
TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC 
TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC 
TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG 
TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT 
GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT 
GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT 
ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC 
CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG 
GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG 
CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG 
ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT 
CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC 
AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG 
GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT 
GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC 
GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA 
GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA 
GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT 
GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA 
GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT 
TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC 
CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC 
CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG 
CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT 
CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC 
TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC 
TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT 
GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA 
ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA 
TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC 
CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC 
TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG 
GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT 
GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT 
GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC 
GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG 
CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG 
GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA 
CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA 
GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC 
CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC 
CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG 
CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC 
ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG 
TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG 
ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA 
CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC 
GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC 
AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC 
TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC 
CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC 
CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG 
GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC 
CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT 
CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG 
GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC 
CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC 
CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC 
GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC 
GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG 
GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG 
GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC 
AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG 
AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC 
GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG 
GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC 
CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG 
GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGGTTCCGC ACACCGTCCT CAACCTGAAG 
GAGCCGCTCT ACGTAGGGGG CGCTCCCGAC TTCAGCAAGC TGGCCCGTGC TGCTGCCGTG 
TCCTCTGGCT TCGACGGTGC CATCCAGCTG GTCTCCCTCG GAGGCCGCCA GCTGCTGACC 
CCGGAGCACG TGCTGCGGCA GGTGGACGTC ACGTCCTTTG CAGGTCACCC CTGCACCCGG 
GCCTCAGGCC ACCCCTGCCT CAATGGGGCC TCCTGCGTCC CGAGGGAGGC TGCCTATGTG 
TGCCTGTGTC CCGGGGGATT CTCAGGACCG CACTGCGAGA AGGGGCTGGT GGAGAAGTCA 
GCGGGGGACG TGGATACCTT GGCCTTTGAC GGGCGGACCT TTGTCGAGTA CCTCAACGCT 
GTGACCGAGA GCGAGAAGGC ACTGCAGAGC AACCACTTTG AACTGAGCCT GCGCACTGAG 
GCCACGCAGG GGCTGGTGCT CTGGAGTGGC AAGGCCACGG AGCGGGCAGA CTATGTGGCA 
CTGGCCATTG TGGACGGGCA CCTGCAACTG AGCTACAACC TGGGCTCCCA GCCCGTGGTG 
CTGCGTTCCA CCGTGCCCGT CAACACCAAC CGCTGGTTGC GGGTCGTGGC ACATAGGGAG 
CAGAGGGAAG GTTCCCTGCA GGTGGGCAAT GAGGCCCCTG TGACCGGCTC CTCCCCGCTG 
GGCGCCACGC AGCTGGACAC TGATGGAGCC CTGTGGCTTG GGGGCCTGCC GGAGCTGCCC 
GTGGGCCCAG CACTGCCCAA GGCCTACGGC ACAGGCTTTG TGGGCTGCTT GCGGGACGTG 
GTGGTGGGCC GGCACCCGCT GCACCTGCTG GAGGACGCCG TCACCAAGCC AGAGCTGCGG 
CCCTGCCCCA CCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_940978.2
CDS	51..6188
Translation	MAGRSHPGPLRPLLPLLVVAACVLPGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKAYGTGFVGCLRDVVVGRHPLHLLEDAVTKPELRPCPTP

Target ORF information:

RefSeq Version	NM_198576.3
Organism	Homo sapiens(human)
Definition	Homo sapiens agrin (AGRN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198576.3

ORF Insert Sequence:

ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC 
GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG 
GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG 
CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG 
GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC 
CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA 
CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG 
ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC 
TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC 
GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC 
CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA 
TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG 
CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG 
CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC 
GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC 
CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT 
CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT 
GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC 
GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC 
CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG 
TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC 
TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC 
TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG 
TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT 
GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT 
GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT 
ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC 
CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG 
GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG 
CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG 
ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT 
CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC 
AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG 
GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT 
GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC 
GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA 
GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA 
GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT 
GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA 
GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT 
TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC 
CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC 
CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG 
CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT 
CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC 
TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC 
TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT 
GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA 
ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA 
TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC 
CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC 
TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG 
GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT 
GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT 
GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC 
GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG 
CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG 
GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA 
CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA 
GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC 
CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC 
CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG 
CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC 
ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG 
TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG 
ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA 
CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC 
GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC 
AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC 
TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC 
CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC 
CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG 
GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC 
CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT 
CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG 
GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC 
CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC 
CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC 
GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC 
GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG 
GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG 
GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC 
AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG 
AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC 
GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG 
GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC 
CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG 
GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGGTTCCGC ACACCGTCCT CAACCTGAAG 
GAGCCGCTCT ACGTAGGGGG CGCTCCCGAC TTCAGCAAGC TGGCCCGTGC TGCTGCCGTG 
TCCTCTGGCT TCGACGGTGC CATCCAGCTG GTCTCCCTCG GAGGCCGCCA GCTGCTGACC 
CCGGAGCACG TGCTGCGGCA GGTGGACGTC ACGTCCTTTG CAGGTCACCC CTGCACCCGG 
GCCTCAGGCC ACCCCTGCCT CAATGGGGCC TCCTGCGTCC CGAGGGAGGC TGCCTATGTG 
TGCCTGTGTC CCGGGGGATT CTCAGGACCG CACTGCGAGA AGGGGCTGGT GGAGAAGTCA 
GCGGGGGACG TGGATACCTT GGCCTTTGAC GGGCGGACCT TTGTCGAGTA CCTCAACGCT 
GTGACCGAGA GCGAGAAGGC ACTGCAGAGC AACCACTTTG AACTGAGCCT GCGCACTGAG 
GCCACGCAGG GGCTGGTGCT CTGGAGTGGC AAGGCCACGG AGCGGGCAGA CTATGTGGCA 
CTGGCCATTG TGGACGGGCA CCTGCAACTG AGCTACAACC TGGGCTCCCA GCCCGTGGTG 
CTGCGTTCCA CCGTGCCCGT CAACACCAAC CGCTGGTTGC GGGTCGTGGC ACATAGGGAG 
CAGAGGGAAG GTTCCCTGCA GGTGGGCAAT GAGGCCCCTG TGACCGGCTC CTCCCCGCTG 
GGCGCCACGC AGCTGGACAC TGATGGAGCC CTGTGGCTTG GGGGCCTGCC GGAGCTGCCC 
GTGGGCCCAG CACTGCCCAA GGCCTACGGC ACAGGCTTTG TGGGCTGCTT GCGGGACGTG 
GTGGTGGGCC GGCACCCGCT GCACCTGCTG GAGGACGCCG TCACCAAGCC AGAGCTGCGG 
CCCTGCCCCA CCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGRN ( XM_011541429.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539731.1 Homo sapiens agrin (AGRN), transcript variant X3, mRNA.

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CloneID	OHu79802
Clone ID Related Accession (Same CDS sequence)	XM_011541429.2
Accession Version	XM_011541429.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	agrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC 
GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG 
GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG 
CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG 
GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC 
CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA 
CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG 
ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC 
TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC 
GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC 
CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA 
TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG 
CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG 
CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC 
GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC 
CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT 
CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT 
GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC 
GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC 
CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG 
TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC 
TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC 
TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG 
TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT 
GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT 
GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT 
ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC 
CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG 
GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG 
CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG 
ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT 
CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC 
AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG 
GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT 
GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC 
GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA 
GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA 
GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT 
GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA 
GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT 
TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC 
CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC 
CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG 
CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT 
CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC 
TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC 
TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT 
GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA 
ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA 
TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC 
CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC 
TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG 
GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT 
GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT 
GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC 
GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG 
CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG 
GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA 
CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA 
GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC 
CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC 
CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG 
CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC 
ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG 
TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG 
ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA 
CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC 
GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC 
AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC 
TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC 
CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC 
CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG 
GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC 
CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT 
CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG 
GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC 
CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC 
CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC 
GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC 
GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG 
GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG 
GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC 
AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG 
AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC 
GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG 
GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC 
CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG 
GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGAAATCCC GCAAGGTTCC GCACACCGTC 
CTCAACCTGA AGGAGCCGCT CTACGTAGGG GGCGCTCCCG ACTTCAGCAA GCTGGCCCGT 
GCTGCTGCCG TGTCCTCTGG CTTCGACGGT GCCATCCAGC TGGTCTCCCT CGGAGGCCGC 
CAGCTGCTGA CCCCGGAGCA CGTGCTGCGG CAGGTGGACG TCACGTCCTT TGCAGGTCAC 
CCCTGCACCC GGGCCTCAGG CCACCCCTGC CTCAATGGGG CCTCCTGCGT CCCGAGGGAG 
GCTGCCTATG TGTGCCTGTG TCCCGGGGGA TTCTCAGGAC CGCACTGCGA GAAGGGGCTG 
GTGGAGAAGT CAGCGGGGGA CGTGGATACC TTGGCCTTTG ACGGGCGGAC CTTTGTCGAG 
TACCTCAACG CTGTGACCGA GAGCGAGAAG GCACTGCAGA GCAACCACTT TGAACTGAGC 
CTGCGCACTG AGGCCACGCA GGGGCTGGTG CTCTGGAGTG GCAAGGCCAC GGAGCGGGCA 
GACTATGTGG CACTGGCCAT TGTGGACGGG CACCTGCAAC TGAGCTACAA CCTGGGCTCC 
CAGCCCGTGG TGCTGCGTTC CACCGTGCCC GTCAACACCA ACCGCTGGTT GCGGGTCGTG 
GCACATAGGG AGCAGAGGGA AGGTTCCCTG CAGGTGGGCA ATGAGGCCCC TGTGACCGGC 
TCCTCCCCGC TGGGCGCCAC GCAGCTGGAC ACTGATGGAG CCCTGTGGCT TGGGCCTTTC 
CAAGCCCCCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539731.1
CDS	72..6086
Translation	MAGRSHPGPLRPLLPLLVVAACVLPGAGGTCPERALERREEEANVVLTGTVEEILNVDPVQHTYSCKVRVWRYLKGKDLVARESLLDGGNKVVISGFGDPLICDNQVSTGDTRIFFVNPAPPYLWPAHKNELMLNSSLMRITLRNLEEVEFCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKKSPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPEPCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSSLYDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGCCQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPMLTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLLLSQALPAPPGALPLAPSSTAHSQTTPPPSSRPRTTASVPRTTVWPVLTVPPTAPSPAPSLVASAFGESGSTDGSSDEELSGDQEASGGGSGGLEPLEGSSVATPGPPVERASCYNSALGCCSDGKTPSLDAEGSNCPATKVFQGVLELEGVEGQELFYTPEMADPKSELFGETARSIESTLDDLFRNSDVKKDFRSVRLRDLGPGKSVRAIVDVHFDPTTAFRAPDVARALLRQIQVSRRRSLGVRRPLQEHVRFMDFDWFPAFITGATSGAIAAGATARATTASRLPSSAVTPRAPHPSHTSQPVAKTTAAPTTRRPPTTAPSRVPGRRPPAPQQPPKPCDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGAPVPAFEGRSFLAFPTLRAYHTLRLALEFRALEPQGLLLYNGNARGKDFLALALLDGRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGIGPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPDFSKLARAAAVSSGFDGAIQLVSLGGRQLLTPEHVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYLNAVTESEKALQSNHFELSLRTEATQGLVLWSGKATERADYVALAIVDGHLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGPFQAPI

Target ORF information:

RefSeq Version	XM_011541429.2
Organism	Homo sapiens(human)
Definition	Homo sapiens agrin (AGRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011541429.2

ORF Insert Sequence:

ATGGCCGGCC GGTCCCACCC GGGCCCGCTG CGGCCGCTGC TGCCGCTCCT TGTGGTGGCC 
GCGTGCGTCC TGCCCGGAGC CGGCGGGACA TGCCCGGAGC GCGCGCTGGA GCGGCGCGAG 
GAGGAGGCGA ACGTGGTGCT CACCGGGACG GTGGAGGAGA TCCTCAACGT GGACCCGGTG 
CAGCACACGT ACTCCTGCAA GGTTCGGGTC TGGCGGTACT TGAAGGGCAA AGACCTGGTG 
GCCCGGGAGA GCCTGCTGGA CGGCGGCAAC AAGGTGGTGA TCAGCGGCTT TGGAGACCCC 
CTCATCTGTG ACAACCAGGT GTCCACTGGG GACACCAGGA TCTTCTTTGT GAACCCTGCA 
CCCCCATACC TGTGGCCAGC CCACAAGAAC GAGCTGATGC TCAACTCCAG CCTCATGCGG 
ATCACCCTGC GGAACCTGGA GGAGGTGGAG TTCTGTGTGG AAGATAAACC CGGGACCCAC 
TTCACTCCAG TGCCTCCGAC GCCTCCTGAT GCGTGCCGGG GAATGCTGTG CGGCTTCGGC 
GCCGTGTGCG AGCCCAACGC GGAGGGGCCG GGCCGGGCGT CCTGCGTCTG CAAGAAGAGC 
CCGTGCCCCA GCGTGGTGGC GCCTGTGTGT GGGTCGGACG CCTCCACCTA CAGCAACGAA 
TGCGAGCTGC AGCGGGCGCA GTGCAGCCAG CAGCGCCGCA TCCGCCTGCT CAGCCGCGGG 
CCGTGCGGCT CGCGGGACCC CTGCTCCAAC GTGACCTGCA GCTTCGGCAG CACCTGTGCG 
CGCTCGGCCG ACGGGCTGAC GGCCTCGTGC CTGTGCCCCG CGACCTGCCG TGGCGCCCCC 
GAGGGGACCG TCTGCGGCAG CGACGGCGCC GACTACCCCG GCGAGTGCCA GCTCCTGCGC 
CGCGCCTGCG CCCGCCAGGA GAATGTCTTC AAGAAGTTCG ACGGCCCTTG TGACCCCTGT 
CAGGGCGCCC TCCCTGACCC GAGCCGCAGC TGCCGTGTGA ACCCGCGCAC GCGGCGCCCT 
GAGATGCTCC TACGGCCCGA GAGCTGCCCT GCCCGGCAGG CGCCAGTGTG TGGGGACGAC 
GGAGTCACCT ACGAAAACGA CTGTGTCATG GGCCGATCGG GGGCCGCCCG GGGTCTCCTC 
CTGCAGAAAG TGCGCTCCGG CCAGTGCCAG GGTCGAGACC AGTGCCCGGA GCCCTGCCGG 
TTCAATGCCG TGTGCCTGTC CCGCCGTGGC CGTCCCCGCT GCTCCTGCGA CCGCGTCACC 
TGTGACGGGG CCTACAGGCC CGTGTGTGCC CAGGACGGGC GCACGTATGA CAGTGATTGC 
TGGCGGCAGC AGGCTGAGTG CCGGCAGCAG CGTGCCATCC CCAGCAAGCA CCAGGGCCCG 
TGTGACCAGG CCCCGTCCCC ATGCCTCGGG GTGCAGTGTG CATTTGGGGC GACGTGTGCT 
GTGAAGAACG GGCAGGCAGC GTGTGAATGC CTGCAGGCGT GCTCGAGCCT CTACGATCCT 
GTGTGCGGCA GCGACGGCGT CACATACGGC AGCGCGTGCG AGCTGGAGGC CACGGCCTGT 
ACCCTCGGGC GGGAGATCCA GGTGGCGCGC AAAGGACCCT GTGACCGCTG CGGGCAGTGC 
CGCTTTGGAG CCCTGTGCGA GGCCGAGACC GGGCGCTGCG TGTGCCCCTC TGAATGCGTG 
GCTTTGGCCC AGCCCGTGTG TGGCTCCGAC GGGCACACGT ACCCCAGCGA GTGCATGCTG 
CACGTGCACG CCTGCACACA CCAGATCAGC CTGCACGTGG CCTCAGCTGG ACCCTGTGAG 
ACCTGTGGAG ATGCCGTGTG TGCTTTTGGG GCTGTGTGCT CCGCAGGGCA GTGTGTGTGT 
CCCCGGTGTG AGCACCCCCC GCCCGGCCCC GTGTGTGGCA GCGACGGTGT CACCTACGGC 
AGTGCCTGCG AGCTACGGGA AGCCGCCTGC CTCCAGCAGA CACAGATCGA GGAGGCCCGG 
GCAGGGCCGT GCGAGCAGGC CGAGTGCGGT TCCGGAGGCT CTGGCTCTGG GGAGGACGGT 
GACTGTGAGC AGGAGCTGTG CCGGCAGCGC GGTGGCATCT GGGACGAGGA CTCGGAGGAC 
GGGCCGTGTG TCTGTGACTT CAGCTGCCAG AGTGTCCCAG GCAGCCCGGT GTGCGGCTCA 
GATGGGGTCA CCTACAGCAC CGAGTGTGAG CTGAAGAAGG CCAGGTGTGA GTCACAGCGA 
GGGCTCTACG TAGCGGCCCA GGGAGCCTGC CGAGGCCCCA CCTTCGCCCC GCTGCCGCCT 
GTGGCCCCCT TACACTGTGC CCAGACGCCC TACGGCTGCT GCCAGGACAA TATCACCGCA 
GCCCGGGGCG TGGGCCTGGC TGGCTGCCCC AGTGCCTGCC AGTGCAACCC CCATGGCTCT 
TACGGCGGCA CCTGTGACCC AGCCACAGGC CAGTGCTCCT GCCGCCCAGG TGTGGGGGGC 
CTCAGGTGTG ACCGCTGTGA GCCTGGCTTC TGGAACTTTC GAGGCATCGT CACCGATGGC 
CGGAGTGGCT GTACACCCTG CAGCTGTGAT CCCCAAGGCG CCGTGCGGGA TGACTGTGAG 
CAGATGACGG GGCTGTGCTC GTGTAAGCCC GGGGTGGCTG GACCCAAGTG TGGGCAGTGT 
CCAGACGGCC GTGCCCTGGG CCCCGCGGGC TGTGAAGCTG ACGCTTCTGC GCCTGCGACC 
TGTGCGGAGA TGCGCTGTGA GTTCGGTGCG CGGTGCGTGG AGGAGTCTGG CTCAGCCCAC 
TGTGTCTGCC CGATGCTCAC CTGTCCAGAG GCCAACGCTA CCAAGGTCTG TGGGTCAGAT 
GGAGTCACAT ACGGCAACGA GTGTCAGCTG AAGACCATCG CCTGCCGCCA GGGCCTGCAA 
ATCTCTATCC AGAGCCTGGG CCCGTGCCAG GAGGCTGTTG CTCCCAGCAC TCACCCGACA 
TCTGCCTCCG TGACTGTGAC CACCCCAGGG CTCCTCCTGA GCCAGGCACT GCCGGCCCCC 
CCCGGCGCCC TCCCCCTGGC TCCCAGCAGT ACCGCACACA GCCAGACCAC CCCTCCGCCC 
TCATCACGAC CTCGGACCAC TGCCAGCGTC CCCAGGACCA CCGTGTGGCC CGTGCTGACG 
GTGCCCCCCA CGGCACCCTC CCCTGCACCC AGCCTGGTGG CGTCCGCCTT TGGTGAATCT 
GGCAGCACTG ATGGAAGCAG CGATGAGGAA CTGAGCGGGG ACCAGGAGGC CAGTGGGGGT 
GGCTCTGGGG GGCTCGAGCC CTTGGAGGGC AGCAGCGTGG CCACCCCTGG GCCACCTGTC 
GAGAGGGCTT CCTGCTACAA CTCCGCGTTG GGCTGCTGCT CTGATGGGAA GACGCCCTCG 
CTGGACGCAG AGGGCTCCAA CTGCCCCGCC ACCAAGGTGT TCCAGGGCGT CCTGGAGCTG 
GAGGGCGTCG AGGGCCAGGA GCTGTTCTAC ACGCCCGAGA TGGCTGACCC CAAGTCAGAA 
CTGTTCGGGG AGACAGCCAG GAGCATTGAG AGCACCCTGG ACGACCTCTT CCGGAATTCA 
GACGTCAAGA AGGATTTTCG GAGTGTCCGC TTGCGGGACC TGGGGCCCGG CAAATCCGTC 
CGCGCCATTG TGGATGTGCA CTTTGACCCC ACCACAGCCT TCAGGGCACC CGACGTGGCC 
CGGGCCCTGC TCCGGCAGAT CCAGGTGTCC AGGCGCCGGT CCTTGGGGGT GAGGCGGCCG 
CTGCAGGAGC ACGTGCGATT TATGGACTTT GACTGGTTTC CTGCGTTTAT CACGGGGGCC 
ACGTCAGGAG CCATTGCTGC GGGAGCCACG GCCAGAGCCA CCACTGCATC GCGCCTGCCG 
TCCTCTGCTG TGACCCCTCG GGCCCCGCAC CCCAGTCACA CAAGCCAGCC CGTTGCCAAG 
ACCACGGCAG CCCCCACCAC ACGTCGGCCC CCCACCACTG CCCCCAGCCG TGTGCCCGGA 
CGTCGGCCCC CGGCCCCCCA GCAGCCTCCA AAGCCCTGTG ACTCACAGCC CTGCTTCCAC 
GGGGGGACCT GCCAGGACTG GGCATTGGGC GGGGGCTTCA CCTGCAGCTG CCCGGCAGGC 
AGGGGAGGCG CCGTCTGTGA GAAGGTGCTT GGCGCCCCTG TGCCGGCCTT CGAGGGCCGC 
TCCTTCCTGG CCTTCCCCAC TCTCCGCGCC TACCACACGC TGCGCCTGGC ACTGGAATTC 
CGGGCGCTGG AGCCTCAGGG GCTGCTGCTG TACAATGGCA ACGCCCGGGG CAAGGACTTC 
CTGGCATTGG CGCTGCTAGA TGGCCGCGTG CAGCTCAGGT TTGACACAGG TTCGGGGCCG 
GCGGTGCTGA CCAGTGCCGT GCCGGTAGAG CCGGGCCAGT GGCACCGCCT GGAGCTGTCC 
CGGCACTGGC GCCGGGGCAC CCTCTCGGTG GATGGTGAGA CCCCTGTTCT GGGCGAGAGT 
CCCAGTGGCA CCGACGGCCT CAACCTGGAC ACAGACCTCT TTGTGGGCGG CGTACCCGAG 
GACCAGGCTG CCGTGGCGCT GGAGCGGACC TTCGTGGGCG CCGGCCTGAG GGGGTGCATC 
CGTTTGCTGG ACGTCAACAA CCAGCGCCTG GAGCTTGGCA TTGGGCCGGG GGCTGCCACC 
CGAGGCTCTG GCGTGGGCGA GTGCGGGGAC CACCCCTGCC TGCCCAACCC CTGCCATGGC 
GGGGCCCCAT GCCAGAACCT GGAGGCTGGA AGGTTCCATT GCCAGTGCCC GCCCGGCCGC 
GTCGGACCAA CCTGTGCCGA TGAGAAGAGC CCCTGCCAGC CCAACCCCTG CCATGGGGCG 
GCGCCCTGCC GTGTGCTGCC CGAGGGTGGT GCTCAGTGCG AGTGCCCCCT GGGGCGTGAG 
GGCACCTTCT GCCAGACAGC CTCGGGGCAG GACGGCTCTG GGCCCTTCCT GGCTGACTTC 
AACGGCTTCT CCCACCTGGA GCTGAGAGGC CTGCACACCT TTGCACGGGA CCTGGGGGAG 
AAGATGGCGC TGGAGGTCGT GTTCCTGGCA CGAGGCCCCA GCGGCCTCCT GCTCTACAAC 
GGGCAGAAGA CGGACGGCAA GGGGGACTTC GTGTCGCTGG CACTGCGGGA CCGCCGCCTG 
GAGTTCCGCT ACGACCTGGG CAAGGGGGCA GCGGTCATCA GGAGCAGGGA GCCAGTCACC 
CTGGGAGCCT GGACCAGGGT CTCACTGGAG CGAAACGGCC GCAAGGGTGC CCTGCGTGTG 
GGCGACGGCC CCCGTGTGTT GGGGGAGTCC CCGAAATCCC GCAAGGTTCC GCACACCGTC 
CTCAACCTGA AGGAGCCGCT CTACGTAGGG GGCGCTCCCG ACTTCAGCAA GCTGGCCCGT 
GCTGCTGCCG TGTCCTCTGG CTTCGACGGT GCCATCCAGC TGGTCTCCCT CGGAGGCCGC 
CAGCTGCTGA CCCCGGAGCA CGTGCTGCGG CAGGTGGACG TCACGTCCTT TGCAGGTCAC 
CCCTGCACCC GGGCCTCAGG CCACCCCTGC CTCAATGGGG CCTCCTGCGT CCCGAGGGAG 
GCTGCCTATG TGTGCCTGTG TCCCGGGGGA TTCTCAGGAC CGCACTGCGA GAAGGGGCTG 
GTGGAGAAGT CAGCGGGGGA CGTGGATACC TTGGCCTTTG ACGGGCGGAC CTTTGTCGAG 
TACCTCAACG CTGTGACCGA GAGCGAGAAG GCACTGCAGA GCAACCACTT TGAACTGAGC 
CTGCGCACTG AGGCCACGCA GGGGCTGGTG CTCTGGAGTG GCAAGGCCAC GGAGCGGGCA 
GACTATGTGG CACTGGCCAT TGTGGACGGG CACCTGCAAC TGAGCTACAA CCTGGGCTCC 
CAGCCCGTGG TGCTGCGTTC CACCGTGCCC GTCAACACCA ACCGCTGGTT GCGGGTCGTG 
GCACATAGGG AGCAGAGGGA AGGTTCCCTG CAGGTGGGCA ATGAGGCCCC TGTGACCGGC 
TCCTCCCCGC TGGGCGCCAC GCAGCTGGAC ACTGATGGAG CCCTGTGGCT TGGGCCTTTC 
CAAGCCCCCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

JQ1 is a potential therapeutic option for COPD Patients with Agrin overexpression.
American journal of physiology. Lung cellular and molecular physiology(2017 Dec)
Xiao Z,Shu J,Zhou F,Han Y

Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
Journal of proteome research16(8)3083-3091(2017 Aug)
Naba A,Pearce OMT,Del Rosario A,Ma D,Ding H,Rajeeve V,Cutillas PR,Balkwill FR,Hynes RO

C-terminal fragment of agrin (CAF) levels predict acute kidney injury after acute myocardial infarction.
BMC nephrology18(1)202(2017 Jun)
Arampatzis S,Chalikias G,Devetzis V,Konstantinides S,Huynh-Do U,Tziakas D

Agrin as a Mechanotransduction Signal Regulating YAP through the Hippo Pathway.
Cell reports18(10)2464-2479(2017 Mar)
Chakraborty S,Njah K,Pobbati AV,Lim YB,Raju A,Lakshmanan M,Tergaonkar V,Lim CT,Hong W

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AGRN cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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