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KRT6A cDNA ORF clone, Homo sapiens (human)

Gene Symbol KRT6A
Entrez Gene ID 3853
Full Name keratin 6A, type II
Synonyms CK6A, CK6C, CK6D, K6A, K6C, K6D, KRT6C, KRT6D, PC3
General protein information
Preferred Names
keratin, type II cytoskeletal 6A
Names
keratin, type II cytoskeletal 6A
cytokeratin 6A
cytokeratin 6C
cytokeratin 6D
type-II keratin Kb6
keratin, epidermal type II, K6A
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q13.13

Summary The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. The genes are expressed with family members KRT16 and/or KRT17 in the filiform papillae of the tongue, the stratified epithelial lining of oral mucosa and esophagus, the outer root sheath of hair follicles, and the glandular epithelia. This KRT6 gene in particular encodes the most abundant isoform. Mutations in these genes have been associated with pachyonychia congenita. In addition, peptides from the C-terminal region of the protein have antimicrobial activity against bacterial pathogens. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Oct 2014]. lac of sum
Disorder MIM:

148041

Disorder Html: Pachyonychia congenita, Jadassohn-Lewandowsky type, 167200 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_005554 NP_005545 keratin, type II cytoskeletal 6A


Homo sapiens (human) KRT6A NP_005545.1
Pan troglodytes (chimpanzee) KRT6A XP_509078.2
Macaca mulatta (Rhesus monkey) LOC100424162 XP_002798640.1
Canis lupus familiaris (dog) KRT6B XP_003433560.1
Canis lupus familiaris (dog) KRT6A XP_005636851.1
Bos taurus (cattle) KRT6A NP_001076979.1
Bos taurus (cattle) KRT6B NP_001244333.1
Bos taurus (cattle) KRT6C XP_002687296.1
Gallus gallus (chicken) KRT3 XP_004949798.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KRT6A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT6A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu19164
NM_005554 Homo sapiens keratin 6A, type II (KRT6A), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu19164
Clone ID Related Accession (Same CDS sequence) NM_005554
Accession Version NM_005554.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1695bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product keratin, type II cytoskeletal 6A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Michael Rogers. The reference sequence was derived from DA451441.1, BC008807.2 and BG059253.1. This sequence is a reference standard in the RefSeqGene project. On or before Feb 27, 2007 this sequence version replaced gi:17505188, gi:17505186. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 23006328 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC014152.2, BC008807.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCCAGCA CATCCACCAC CATCAGGAGC CACAGCAGCA GCCGCCGGGG TTTCAGTGCC 
AACTCAGCCA GGCTCCCTGG GGTCAGCCGC TCTGGCTTCA GCAGCGTCTC CGTGTCCCGC
TCCAGGGGCA GTGGTGGCCT GGGTGGTGCA TGTGGAGGAG CTGGCTTTGG CAGCCGCAGT
CTGTATGGCC TGGGGGGCTC CAAGAGGATC TCCATTGGAG GGGGCAGCTG TGCCATCAGT
GGCGGCTATG GCAGCAGAGC CGGAGGCAGC TATGGCTTTG GTGGCGCCGG GAGTGGATTT
GGTTTCGGTG GTGGAGCCGG CATTGGCTTT GGTCTGGGTG GTGGAGCCGG CCTTGCTGGT
GGCTTTGGGG GCCCTGGCTT CCCTGTGTGC CCCCCTGGAG GCATCCAAGA GGTCACCGTC
AACCAGAGTC TCCTGACTCC CCTCAACCTG CAAATCGATC CCACCATCCA GCGGGTGCGG
GCTGAGGAGC GTGAACAGAT CAAGACCCTC AACAACAAGT TTGCCTCCTT CATCGACAAG
GTGCGGTTCC TGGAGCAGCA GAACAAGGTT CTGGAAACAA AGTGGACCCT GCTGCAGGAG
CAGGGCACCA AGACTGTGAG GCAGAACCTG GAGCCGTTGT TCGAGCAGTA CATCAACAAC
CTCAGGAGGC AGCTGGACAG CATTGTCGGG GAACGGGGCC GCCTGGACTC AGAGCTCAGA
GGCATGCAGG ACCTGGTGGA GGACTTCAAG AACAAATATG AGGATGAAAT CAACAAGCGC
ACAGCAGCAG AGAATGAATT TGTGACTCTG AAGAAGGATG TGGATGCTGC CTACATGAAC
AAGGTTGAAC TGCAAGCCAA GGCAGACACT CTCACAGACG AGATCAACTT CCTGAGAGCC
TTGTATGATG CAGAGCTGTC CCAGATGCAG ACCCACATCT CAGACACATC TGTGGTGCTG
TCCATGGACA ACAACCGCAA CCTGGACCTG GACAGCATCA TCGCTGAGGT CAAGGCCCAA
TATGAGGAGA TTGCTCAGAG AAGCCGGGCT GAGGCTGAGT CCTGGTACCA GACCAAGTAC
GAGGAGCTGC AGGTCACAGC AGGCAGACAT GGGGACGACC TGCGCAACAC CAAGCAGGAG
ATTGCTGAGA TCAACCGCAT GATCCAGAGG CTGAGATCTG AGATCGACCA CGTCAAGAAG
CAGTGCGCCA ACCTGCAGGC CGCCATTGCT GATGCTGAGC AGCGTGGGGA GATGGCCCTC
AAGGATGCCA AGAACAAGCT GGAAGGGCTG GAGGATGCCC TGCAGAAGGC CAAGCAGGAC
CTGGCCCGGC TGCTGAAGGA GTACCAGGAG CTGATGAATG TCAAGCTGGC CCTGGACGTG
GAGATCGCCA CCTACCGCAA GCTGCTGGAG GGTGAGGAGT GCAGGCTGAA TGGCGAAGGC
GTTGGACAAG TCAACATCTC TGTGGTGCAG TCCACCGTCT CCAGTGGCTA TGGCGGTGCC
AGTGGTGTCG GCAGTGGCTT AGGCCTGGGT GGAGGAAGCA GCTACTCCTA TGGCAGTGGT
CTTGGCGTTG GAGGTGGCTT CAGTTCCAGC AGTGGCAGAG CCATTGGGGG TGGCCTCAGC
TCTGTTGGAG GCGGCAGTTC CACCATCAAG TACACCACCA CCTCCTCCTC CAGCAGGAAG
AGCTATAAGC ACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005545.1
CDS210..1904
Misc Feature(1)114..116(+)
Misc Feature(2)213..695(+)
Misc Feature(3)213..215(+)
Misc Feature(4)693..1634(+)
Misc Feature(5)696..1625(+)
Misc Feature(6)696..803(+)
Misc Feature(7)804..860(+)
Misc Feature(8)861..1136(+)
Misc Feature(9)1137..1208(+)
Misc Feature(10)1209..1625(+)
Misc Feature(11)1317..>1652(+)
Misc Feature(12)1449..1451(+)
Misc Feature(13)1626..1901(+)
Exon (1)1..749
Gene:KRT6A
Gene Synonym:
Exon (2)750..964
Gene:KRT6A
Gene Synonym:
Exon (3)965..1025
Gene:KRT6A
Gene Synonym:
Exon (4)1026..1121
Gene:KRT6A
Gene Synonym:
Exon (5)1122..1286
Gene:KRT6A
Gene Synonym:
Exon (6)1287..1412
Gene:KRT6A
Gene Synonym:
Exon (7)1413..1633
Gene:KRT6A
Gene Synonym:
Exon (8)1634..1668
Gene:KRT6A
Gene Synonym:
Exon (9)1669..2450
Gene:KRT6A
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005554
Organism Homo sapiens (human)
Definition Homo sapiens keratin 6A, type II (KRT6A), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005554

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGGCCAGCA CATCCACCAC CATCAGGAGC CACAGCAGCA GCCGCCGGGG TTTCAGTGCC 
AACTCAGCCA GGCTCCCTGG GGTCAGCCGC TCTGGCTTCA GCAGCGTCTC CGTGTCCCGC
TCCAGGGGCA GTGGTGGCCT GGGTGGTGCA TGTGGAGGAG CTGGCTTTGG CAGCCGCAGT
CTGTATGGCC TGGGGGGCTC CAAGAGGATC TCCATTGGAG GGGGCAGCTG TGCCATCAGT
GGCGGCTATG GCAGCAGAGC CGGAGGCAGC TATGGCTTTG GTGGCGCCGG GAGTGGATTT
GGTTTCGGTG GTGGAGCCGG CATTGGCTTT GGTCTGGGTG GTGGAGCCGG CCTTGCTGGT
GGCTTTGGGG GCCCTGGCTT CCCTGTGTGC CCCCCTGGAG GCATCCAAGA GGTCACCGTC
AACCAGAGTC TCCTGACTCC CCTCAACCTG CAAATCGATC CCACCATCCA GCGGGTGCGG
GCTGAGGAGC GTGAACAGAT CAAGACCCTC AACAACAAGT TTGCCTCCTT CATCGACAAG
GTGCGGTTCC TGGAGCAGCA GAACAAGGTT CTGGAAACAA AGTGGACCCT GCTGCAGGAG
CAGGGCACCA AGACTGTGAG GCAGAACCTG GAGCCGTTGT TCGAGCAGTA CATCAACAAC
CTCAGGAGGC AGCTGGACAG CATTGTCGGG GAACGGGGCC GCCTGGACTC AGAGCTCAGA
GGCATGCAGG ACCTGGTGGA GGACTTCAAG AACAAATATG AGGATGAAAT CAACAAGCGC
ACAGCAGCAG AGAATGAATT TGTGACTCTG AAGAAGGATG TGGATGCTGC CTACATGAAC
AAGGTTGAAC TGCAAGCCAA GGCAGACACT CTCACAGACG AGATCAACTT CCTGAGAGCC
TTGTATGATG CAGAGCTGTC CCAGATGCAG ACCCACATCT CAGACACATC TGTGGTGCTG
TCCATGGACA ACAACCGCAA CCTGGACCTG GACAGCATCA TCGCTGAGGT CAAGGCCCAA
TATGAGGAGA TTGCTCAGAG AAGCCGGGCT GAGGCTGAGT CCTGGTACCA GACCAAGTAC
GAGGAGCTGC AGGTCACAGC AGGCAGACAT GGGGACGACC TGCGCAACAC CAAGCAGGAG
ATTGCTGAGA TCAACCGCAT GATCCAGAGG CTGAGATCTG AGATCGACCA CGTCAAGAAG
CAGTGCGCCA ACCTGCAGGC CGCCATTGCT GATGCTGAGC AGCGTGGGGA GATGGCCCTC
AAGGATGCCA AGAACAAGCT GGAAGGGCTG GAGGATGCCC TGCAGAAGGC CAAGCAGGAC
CTGGCCCGGC TGCTGAAGGA GTACCAGGAG CTGATGAATG TCAAGCTGGC CCTGGACGTG
GAGATCGCCA CCTACCGCAA GCTGCTGGAG GGTGAGGAGT GCAGGCTGAA TGGCGAAGGC
GTTGGACAAG TCAACATCTC TGTGGTGCAG TCCACCGTCT CCAGTGGCTA TGGCGGTGCC
AGTGGTGTCG GCAGTGGCTT AGGCCTGGGT GGAGGAAGCA GCTACTCCTA TGGCAGTGGT
CTTGGCGTTG GAGGTGGCTT CAGTTCCAGC AGTGGCAGAG CCATTGGGGG TGGCCTCAGC
TCTGTTGGAG GCGGCAGTTC CACCATCAAG TACACCACCA CCTCCTCCTC CAGCAGGAAG
AGCTATAAGC ACTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Cytokeratin 5/6, c-Met expressions, and PTEN loss prognostic indicators in triple-negative breast cancer
Med. Oncol. 31 (1), 801 (2014)
Inanc M, Ozkan M, Karaca H, Berk V, Bozkurt O, Duran AO, Ozaslan E, Akgun H, Tekelioglu F and Elmali F.


book

Cytokeratins mediate epithelial innate defense through their antimicrobial properties
J. Clin. Invest. 122 (10), 3665-3677 (2012)
Tam C, Mun JJ, Evans DJ and Fleiszig SM.


book

A review of the clinical phenotype of 254 patients with genetically confirmed pachyonychia congenita
J. Am. Acad. Dermatol. 67 (4), 680-686 (2012)
Eliason MJ, Leachman SA, Feng BJ, Schwartz ME and Hansen CD.


book

Two novel de novo mutations of KRT6A and KRT16 genes in two Chinese pachyonychia congenita pedigrees with fissured tongue or diffuse plantar keratoderma
Eur J Dermatol 22 (4), 476-480 (2012)
Du ZF, Xu CM, Zhao Y, Liu WT, Chen XL, Chen CY, Fang H, Ke HP and Zhang XN.


book

The diagnostic utility of D2-40, calretinin, CK5/6, desmin and MOC-31 in the differentiation of mesothelioma from adenocarcinoma in pleural effusion cytology
Acta Cytol. 56 (5), 527-532 (2012)
Hyun TS, Barnes M and Tabatabai ZL.


book

Cloning and characterization of multiple human genes and cDNAs encoding highly related type II keratin 6 isoforms
J. Biol. Chem. 270 (31), 18581-18592 (1995)
Takahashi K, Paladini RD and Coulombe PA.


book

Making a connection: direct binding between keratin intermediate filaments and desmosomal proteins
J. Cell Biol. 127 (4), 1049-1060 (1994)
Kouklis PD, Hutton E and Fuchs E.


book

Pachyonychia Congenita
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Smith,F.J.D., Hansen,C.D., Hull,P.R., Kaspar,R.L., Schwartz,M.E., McLean,W.H.I., Milstone,L.M., van Steensel,M., O'Toole,E. and Sprecher,E.


book

Three epidermal and one simple epithelial type II keratin genes map to human chromosome 12
Cytogenet. Cell Genet. 57 (1), 33-38 (1991)
Rosenberg M, Fuchs E, Le Beau MM, Eddy RL and Shows TB.


book

The cDNA sequence of a Type II cytoskeletal keratin reveals constant and variable structural domains among keratins
Cell 33 (3), 915-924 (1983)
Hanukoglu,I. and Fuchs,E.


 
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