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LAMB2 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol LAMB2
Entrez Gene ID 3913
Full Name laminin subunit beta 2
Synonyms LAMS,NPHS5
General protein information
Preferred Names

laminin subunit beta 2

Names

laminin subunit beta-2
S-LAM beta
S-laminin subunit beta
laminin B1s chain
laminin S
laminin, beta 2 (laminin S)

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

3

3p21.31

Summary Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 2. The beta 2 chain contains the 7 structural domains typical of beta chains of laminin, including the short alpha region. However, unlike beta 1 chain, beta 2 has a more restricted tissue distribution. It is enriched in the basement membrane of muscles at the neuromuscular junctions, kidney glomerulus and vascular smooth muscle. Transgenic mice in which the beta 2 chain gene was inactivated by homologous recombination, showed defects in the maturation of neuromuscular junctions and impairment of glomerular filtration. Alternative splicing involving a non consensus 5' splice site (gc) in the 5' UTR of this gene has been reported. It was suggested that inefficient splicing of this first intron, which does not change the protein sequence, results in a greater abundance of the unspliced form of the transcript than the spliced form. The full-length nature of the spliced transcript is not known. [provided by RefSeq, Aug 2011].
Disorder MIM:

150325

Disorder Html: Nephrosis, congenital, with or without ocular abnormalities, 609049

mRNA and Protein(s)

mRNA Protein Name
XM_005265127.3 XP_005265184.1 laminin subunit beta-2 isoform X1
NM_002292.3 NP_002283.3 laminin subunit beta-2 precursor



Gallus gallus (chicken) LAMB2 NP_989497.1
Drosophila melanogaster (fruit fly) LanB1 NP_723319.1
Danio rerio (zebrafish) lamb2 NP_001229974.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP001381 XP_321757.5
Caenorhabditis elegans (roundworm) lam-1 NP_500734.3
Mus musculus (house mouse) Lamb2 NP_032509.2
Homo sapiens (human) LAMB2 NP_002283.3
Pan troglodytes (chimpanzee) LAMB2 XP_003309859.1
Bos taurus (cattle) LAMB2 NP_001039712.2
Canis lupus familiaris (dog) LAMB2 XP_005632610.1
Rattus norvegicus (Norway rat) Lamb2 NP_037106.1
Xenopus tropicalis (tropical clawed frog) lamb2 XP_002933101.1
Macaca mulatta (rhesus monkey) LAMB2 XP_001110027.1


Related articles in PubMed

LAMB2 mutation with different phenotypes in China
.
Zhang H, Cui J, Wang F, Xiao H, Ding J, Yao Y
Clinical nephrology87 (2017)(1)33-38(2017 Jan)

Laminins 411 and 421 differentially promote tumor cell migration via α6β1 integrin and MCAM (CD146).
Ishikawa T, Wondimu Z, Oikawa Y, Gentilcore G, Kiessling R, Egyhazi Brage S, Hansson J, Patarroyo M
Matrix biology : journal of the International Society for Matrix Biology3869-83(2014 Sep)

Retrospective mutational analysis of NPHS1, NPHS2, WT1 and LAMB2 in children with steroid-resistant focal segmental glomerulosclerosis - a single-centre experience.
Bińczak-Kuleta A, Rubik J, Litwin M, Ryder M, Lewandowska K, Taryma-Leśniak O, Clark JS, Grenda R, Ciechanowicz A
Bosnian journal of basic medical sciences14(2)89-93(2014 May)

Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury.
Barallobre-Barreiro J, Didangelos A, Schoendube FA, Drozdov I, Yin X, Fernández-Caggiano M, Willeit P, Puntmann VO, Aldama-López G, Shah AM, Doménech N, Mayr M
Circulation125(6)789-802(2012 Feb)

A novel mutation of LAMB2 in a multigenerational mennonite family reveals a new phenotypic variant of Pierson syndrome.
Mohney BG, Pulido JS, Lindor NM, Hogan MC, Consugar MB, Peters J, Pankratz VS, Nasr SH, Smith SJ, Gloor J, Kubly V, Spencer D, Nielson R, Puffenberger EG, Strauss KA, Morton DH, Eldahdah L, Harris PC
Ophthalmology118(6)1137-44(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In conclusion, we reported three Chinese cases with different LAMB2 mutations and different phenotypes, further broadening the range of eye and kidney pathology associated with mutations in LAMB2.
Title: LAMB2 mutation with different phenotypes in China
.

Laminins 411 and 421 differentially promote tumor cell migration via alpha6beta1 integrin and MCAM (CD146).
Title: Laminins 411 and 421 differentially promote tumor cell migration via α6β1 integrin and MCAM (CD146).

No pathogenic LAMB2 mutations were found in the cohort of children with steroid-resistant focal segmental glomerulosclerosis.
Title: Retrospective mutational analysis of NPHS1, NPHS2, WT1 and LAMB2 in children with steroid-resistant focal segmental glomerulosclerosis - a single-centre experience.

Comprehensive gene sequencing revealed a novel LAMB2 variant (c.440A --> G; His147R) that was homozygous in the 9 living, affected family members, observed at a frequency of 2.1% in the Old Order Mennonite population, and absent in 91 non-Mennonite controls
Title: A novel mutation of LAMB2 in a multigenerational mennonite family reveals a new phenotypic variant of Pierson syndrome.

Novel mutations in steroid-resistant nephrotic syndrome diagnosed in Tunisian children were detected in LAMB2.
Title: Novel mutations in steroid-resistant nephrotic syndrome diagnosed in Tunisian children.

The following LAMB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LAMB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu17891 NM_002292.3
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Homo sapiens laminin subunit beta 2 (LAMB2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $923.30
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OHu17891 XM_005265127.3
Latest version!
Homo sapiens laminin subunit beta 2 (LAMB2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $923.30
$1319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu17891
Clone ID Related Accession (Same CDS sequence) XM_005265127.3 , NM_002292.3
Accession Version NM_002292.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5397bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-29
Organism Homo sapiens(Human)
Product laminin subunit beta-2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC135506.3, BP354643.1, CN265778.1, S77512.1, CV814167.1, X79683.1 and CN265784.1. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2006 this sequence version replaced NM_002292.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.286762.1, SRR1660807.197004.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
ATGGAGCTGA CCTCAAGGGA AAGAGGGAGG GGACAGCCTC TGCCCTGGGA ACTTCGACTG 
GGCCTACTGC TAAGCGTGCT GGCTGCCACA CTGGCACAGG CCCCTGCCCC GGATGTGCCT
GGCTGTTCCA GGGGAAGCTG CTACCCCGCC ACGGGCGACC TGCTGGTGGG CCGAGCTGAC
AGACTGACTG CCTCATCCAC TTGTGGCCTG AATGGCCCCC AGCCCTACTG CATCGTCAGT
CACCTGCAGG ACGAAAAGAA GTGCTTCCTT TGTGACTCCC GGCGCCCCTT CTCTGCTAGA
GACAACCCAC ACAGCCATCG CATCCAGAAT GTAGTCACCA GCTTTGCACC ACAGCGGCGG
GCAGCCTGGT GGCAGTCAGA GAATGGTATC CCTGCGGTCA CCATCCAGCT GGACCTGGAG
GCTGAGTTTC ATTTCACACA CCTCATTATG ACCTTCAAGA CATTTCGCCC TGCTGCCATG
CTGGTGGAAC GCTCAGCAGA CTTTGGCCGC ACCTGGCATG TGTACCGATA TTTCTCCTAT
GACTGTGGGG CTGACTTCCC AGGAGTCCCA CTAGCACCCC CACGGCACTG GGATGATGTA
GTCTGTGAGT CCCGCTACTC AGAGATTGAG CCATCCACTG AAGGCGAGGT CATCTATCGT
GTGCTGGACC CTGCCATCCC TATCCCAGAC CCCTACAGCT CACGGATTCA GAACCTGTTG
AAGATCACCA ACCTACGGGT GAACCTGACT CGTCTACACA CGTTGGGAGA CAACCTACTC
GACCCACGGA GGGAGATCCG AGAGAAGTAC TACTATGCCC TCTATGAGCT GGTTGTACGT
GGCAACTGCT TCTGCTACGG ACACGCCTCA GAGTGTGCAC CCGCCCCAGG GGCACCAGCC
CATGCTGAGG GCATGGTGCA CGGAGCTTGC ATCTGCAAAC ACAACACACG TGGCCTCAAC
TGCGAGCAGT GTCAGGATTT CTATCGTGAC CTGCCCTGGC GTCCGGCTGA GGACGGCCAT
AGTCATGCCT GTAGGAAGTG TGAGTGCCAT GGGCACACCC ACAGCTGCCA CTTCGACATG
GCCGTATACC TGGCATCTGG CAATGTGAGT GGAGGTGTGT GTGATGGATG TCAGCATAAC
ACAGCTGGGC GCCACTGTGA GCTCTGTCGG CCCTTCTTCT ACCGTGACCC AACCAAGGAC
CTGCGGGATC CGGCTGTGTG CCGCTCCTGT GATTGTGACC CCATGGGTTC TCAAGACGGT
GGTCGCTGTG ATTCCCATGA TGACCCTGCA CTGGGACTGG TCTCCGGCCA GTGTCGCTGC
AAAGAACATG TGGTGGGCAC TCGCTGCCAG CAATGCCGTG ATGGCTTCTT TGGGCTCAGC
ATCAGTGACC GTCTGGGCTG CCGGCGATGT CAATGTAATG CACGGGGCAC AGTGCCTGGG
AGCACTCCTT GTGACCCCAA CAGTGGATCC TGTTACTGCA AACGTCTAGT GACTGGACGT
GGATGTGACC GCTGCCTGCC TGGCCACTGG GGCCTGAGCC ACGACCTGCT CGGCTGCCGC
CCCTGTGACT GCGACGTGGG TGGTGCTTTG GATCCCCAGT GTGATGAGGG CACAGGTCAA
TGCCACTGCC GCCAGCACAT GGTTGGGCGA CGCTGTGAGC AGGTGCAACC TGGCTACTTC
CGGCCCTTCC TGGACCACCT AATTTGGGAG GCTGAGGACA CCCGAGGGCA GGTGCTCGAT
GTGGTGGAGC GCCTGGTGAC CCCCGGGGAA ACTCCATCCT GGACTGGCTC AGGCTTCGTG
CGGCTACAGG AAGGTCAGAC CCTGGAGTTC CTGGTGGCCT CTGTGCCGAA GGCTATGGAC
TATGACCTGC TGCTGCGCTT AGAGCCCCAG GTCCCTGAGC AATGGGCAGA GTTGGAACTG
ATTGTGCAGC GTCCAGGGCC TGTGCCTGCC CACAGCCTGT GTGGGCATTT GGTGCCCAAG
GATGATCGCA TCCAAGGGAC TCTGCAACCA CATGCCAGGT ACTTGATATT TCCTAATCCT
GTCTGCCTTG AGCCTGGTAT CTCCTACAAG CTGCATCTGA AGCTGGTACG GACAGGGGGA
AGTGCCCAGC CTGAGACTCC CTACTCTGGA CCTGGCCTGC TCATTGACTC GCTGGTGCTG
CTGCCCCGTG TCCTGGTGCT AGAGATGTTT AGTGGGGGTG ATGCTGCTGC CCTGGAGCGC
CAGGCCACCT TTGAACGCTA CCAATGCCAT GAGGAGGGTC TGGTGCCCAG CAAGACTTCT
CCCTCTGAGG CCTGCGCACC CCTCCTCATC AGCCTGTCCA CCCTCATCTA CAATGGTGCC
CTGCCATGTC AGTGCAACCC TCAAGGTTCA CTGAGTTCTG AGTGCAACCC TCATGGTGGT
CAGTGCCTGT GCAAGCCTGG AGTGGTTGGG CGCCGCTGTG ACCTCTGTGC CCCTGGCTAC
TATGGCTTTG GCCCCACAGG CTGTCAAGCC TGCCAGTGCA GCCACGAGGG GGCACTCAGC
AGTCTCTGTG AAAAGACCAG TGGGCAATGT CTCTGTCGAA CTGGTGCCTT TGGGCTTCGC
TGTGACCGCT GCCAGCGTGG CCAGTGGGGA TTCCCTAGCT GCCGGCCATG TGTCTGCAAT
GGGCATGCAG ATGAGTGCAA CACCCACACA GGCGCTTGCC TGGGCTGCCG TGATCACACA
GGGGGTGAGC ACTGTGAAAG GTGCATTGCT GGTTTCCACG GGGACCCACG GCTGCCATAT
GGGGGCCAGT GCCGGCCCTG TCCCTGTCCT GAAGGCCCTG GGAGCCAACG GCACTTTGCT
ACTTCTTGCC ACCAGGATGA ATATTCCCAG CAGATTGTGT GCCACTGCCG GGCAGGCTAT
ACGGGGCTGC GATGTGAAGC TTGTGCCCCT GGGCACTTTG GGGACCCATC AAGGCCAGGT
GGCCGGTGCC AACTGTGTGA GTGCAGTGGG AACATTGACC CAATGGATCC TGATGCCTGT
GACCCCCACA CGGGGCAATG CCTGCGCTGT TTACACCACA CAGAGGGTCC ACACTGTGCC
CACTGCAAGC CTGGCTTCCA TGGGCAGGCT GCCCGACAGA GCTGTCACCG CTGCACATGC
AACCTGCTGG GCACAAATCC GCAGCAGTGC CCATCTCCTG ACCAGTGCCA CTGTGATCCA
AGCAGTGGGC AGTGCCCATG CCTCCCCAAT GTCCAGGGCC CTAGCTGTGA CCGCTGTGCC
CCCAACTTCT GGAACCTCAC CAGTGGCCAT GGTTGCCAGC CTTGTGCCTG CCACCCAAGC
CGGGCCAGAG GCCCCACCTG CAACGAGTTC ACAGGGCAGT GCCACTGCCG TGCCGGCTTT
GGAGGGCGGA CTTGTTCTGA GTGCCAAGAG CTCCACTGGG GAGACCCTGG GTTGCAGTGC
CATGCCTGTG ATTGTGACTC TCGTGGAATA GATACACCTC AGTGTCACCG CTTCACAGGT
CACTGCAGCT GCCGCCCAGG GGTGTCTGGT GTGCGCTGTG ACCAGTGTGC CCGTGGCTTC
TCAGGAATCT TTCCTGCCTG CCATCCCTGC CATGCATGCT TCGGGGATTG GGACCGAGTG
GTGCAGGACT TGGCAGCCCG TACACAGCGC CTAGAGCAGC GGGCGCAGGA GTTGCAACAG
ACGGGTGTGC TGGGTGCCTT TGAGAGCAGC TTCTGGCACA TGCAGGAGAA GCTGGGCATT
GTGCAGGGCA TCGTAGGTGC CCGCAACACC TCAGCCGCCT CCACTGCACA GCTTGTGGAG
GCCACAGAGG AGCTGCGGCG TGAAATTGGG GAGGCCACTG AGCACCTGAC TCAGCTCGAG
GCAGACCTGA CAGATGTGCA AGATGAGAAC TTCAATGCCA ACCATGCACT AAGTGGTCTG
GAGCGAGATA GGCTTGCACT TAATCTCACA CTGCGGCAGC TCGACCAGCA TCTTGACTTG
CTCAAACATT CAAACTTCCT GGGTGCCTAT GACAGCATCC GGCATGCCCA TAGCCAGTCT
GCAGAGGCAG AACGTCGTGC CAATACCTCA GCCCTGGCAG TACCTAGCCC TGTGAGCAAC
TCGGCAAGTG CTCGGCATCG GACAGAGGCA CTGATGGATG CTCAGAAGGA GGACTTCAAC
AGCAAACACA TGGCCAACCA GCGGGCACTT GGCAAGCTCT CTGCCCATAC CCACACCCTG
AGCCTGACAG ACATAAATGA GCTGGTGTGT GGGGCACCAG GGGATGCACC CTGTGCTACA
AGCCCTTGTG GGGGTGCCGG CTGTCGAGAT GAGGATGGGC AGCCGCGCTG TGGGGGCCTC
AGCTGCAATG GGGCAGCGGC TACAGCAGAC CTAGCACTGG GCCGGGCCCG GCACACACAG
GCAGAGCTGC AGCGGGCACT GGCAGAAGGT GGTAGCATCC TCAGCAGAGT GGCTGAGACT
CGTCGGCAGG CAAGCGAGGC ACAGCAGCGG GCCCAGGCAG CCCTGGACAA GGCTAATGCT
TCCAGGGGAC AGGTGGAACA GGCCAACCAG GAACTTCAAG AACTTATCCA GAGTGTGAAG
GACTTCCTCA ACCAGGAGGG GGCTGATCCT GATAGCATTG AAATGGTGGC CACACGGGTG
CTAGAGCTCT CCATCCCAGC TTCAGCTGAG CAGATCCAGC ACCTGGCGGG TGCGATTGCA
GAGCGAGTCC GGAGCCTGGC AGATGTGGAT GCGATCCTGG CACGTACTGT AGGAGATGTG
CGTCGTGCCG AGCAGCTACT GCAGGATGCA CGGCGGGCAA GGAGCTGGGC TGAGGATGAG
AAACAGAAGG CAGAGACAGT ACAGGCAGCA CTGGAGGAGG CCCAGCGGGC ACAGGGTATT
GCCCAGGGTG CCATCCGGGG GGCAGTGGCT GACACACGGG ACACAGAGCA GACCCTGTAC
CAGGTACAGG AGAGGATGGC AGGTGCAGAG CGGGCACTGA GCTCTGCAGG TGAAAGGGCT
CGGCAGTTGG ATGCTCTCCT GGAGGCTCTG AAATTGAAAC GGGCAGGAAA TAGTCTGGCA
GCCTCTACAG CAGAAGAAAC GGCAGGCAGT GCCCAGGGTC GTGCCCAGGA GGCTGAGCAG
CTGCTACGCG GTCCTCTGGG TGATCAGTAC CAGACGGTGA AGGCCCTAGC TGAGCGCAAG
GCCCAAGGTG TGCTGGCTGC ACAGGCAAGG GCAGAACAAC TGCGGGATGA GGCTCGGGAC
CTGTTGCAAG CCGCTCAGGA CAAGCTGCAG CGGCTACAGG AATTGGAAGG CACCTATGAG
GAAAATGAGC GGGCACTGGA GAGTAAGGCA GCCCAGTTGG ACGGGTTGGA GGCCAGGATG
CGCAGCGTGC TTCAAGCCAT CAACTTGCAG GTGCAGATCT ACAACACCTG CCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002283.3
CDS300..5696
Misc Feature(1)213..298
Misc Feature(2)468..1142
Misc Feature(3)1146..1304
Misc Feature(4)order(1146..1148,1152..1154,1173..1175,1227..1229,1233..1235,1260..1262)
Misc Feature(5)1338..1496
Misc Feature(6)order(1338..1340,1344..1346,1365..1367,1419..1421,1428..1430,1455..1457)
Misc Feature(7)1527..1700
Misc Feature(8)order(1527..1529,1533..1535,1566..1568,1611..1613,1617..1619,1644..1646)
Misc Feature(9)1704..1859
Misc Feature(10)order(1707..1709,1713..1715,1749..1751,1770..1772,1776..1778,1803..1805)
Misc Feature(11)1860..1985
Misc Feature(12)order(1863..1865,1869..1871,1899..1901,1920..1922,1926..1928,1953..1955)
Misc Feature(13)2646..2792
Misc Feature(14)order(2646..2648,2652..2654,2682..2684,2703..2705,2709..2711,2736..2738)
Misc Feature(15)2790..2936
Misc Feature(16)order(2790..2792,2796..2798,2826..2828,2847..2849,2853..2855,2880..2882)
Misc Feature(17)2925..3062
Misc Feature(18)order(2928..2930,2934..2936,2955..2957,2976..2978,2985..2987,3012..3014)
Misc Feature(19)3075..3251
Misc Feature(20)order(3078..3080,3084..3086,3126..3128,3159..3161,3165..3167,3192..3194)
Misc Feature(21)3255..>3383
Misc Feature(22)order(3255..3257,3261..3263,3297..3299,3318..3320,3327..3329,3354..3356)
Misc Feature(23)3408..3578
Misc Feature(24)order(3411..3413,3417..3419,3447..3449,3492..3494,3498..3500,3525..3527)
Misc Feature(25)3582..3728
Misc Feature(26)order(3582..3584,3588..3590,3618..3620,3639..3641,3645..3647,3672..3674)
Misc Feature(27)3726..>3845
Misc Feature(28)order(3726..3728,3732..3734,3762..3764,3783..3785,3789..3791,3816..3818)
Misc Feature(29)3867..4526
Misc Feature(30)4527..4625
Misc Feature(31)4626..5693
Misc Feature(32)4632..5402
Misc Feature(33)<4656..4922
Misc Feature(34)4746..5309
Misc Feature(35)4893..4895
Misc Feature(36)5241..5564
Misc Feature(37)<5337..5675
Exon (1)1..375
Exon (2)376..548
Exon (3)549..684
Exon (4)685..758
Exon (5)759..947
Exon (6)948..1011
Exon (7)1012..1214
Exon (8)1215..1335
Exon (9)1336..1524
Exon (10)1525..1704
Exon (11)1705..1817
Exon (12)1818..1897
Exon (13)1898..2030
Exon (14)2031..2189
Exon (15)2190..2317
Exon (16)2318..2450
Exon (17)2451..2643
Exon (18)2644..2787
Exon (19)2788..3019
Exon (20)3020..3183
Exon (21)3184..3408
Exon (22)3409..3626
Exon (23)3627..3723
Exon (24)3724..4096
Exon (25)4097..4281
Exon (26)4282..4523
Exon (27)4524..4872
Exon (28)4873..5080
Exon (29)5081..5222
Exon (30)5223..5399
Exon (31)5400..5559
Exon (32)5560..5808
Translation

Target ORF information:

RefSeq Version NM_002292.3
Organism Homo sapiens(Human)
Definition Homo sapiens laminin subunit beta 2 (LAMB2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002292.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
ATGGAGCTGA CCTCAAGGGA AAGAGGGAGG GGACAGCCTC TGCCCTGGGA ACTTCGACTG 
GGCCTACTGC TAAGCGTGCT GGCTGCCACA CTGGCACAGG CCCCTGCCCC GGATGTGCCT
GGCTGTTCCA GGGGAAGCTG CTACCCCGCC ACGGGCGACC TGCTGGTGGG CCGAGCTGAC
AGACTGACTG CCTCATCCAC TTGTGGCCTG AATGGCCCCC AGCCCTACTG CATCGTCAGT
CACCTGCAGG ACGAAAAGAA GTGCTTCCTT TGTGACTCCC GGCGCCCCTT CTCTGCTAGA
GACAACCCAC ACAGCCATCG CATCCAGAAT GTAGTCACCA GCTTTGCACC ACAGCGGCGG
GCAGCCTGGT GGCAGTCAGA GAATGGTATC CCTGCGGTCA CCATCCAGCT GGACCTGGAG
GCTGAGTTTC ATTTCACACA CCTCATTATG ACCTTCAAGA CATTTCGCCC TGCTGCCATG
CTGGTGGAAC GCTCAGCAGA CTTTGGCCGC ACCTGGCATG TGTACCGATA TTTCTCCTAT
GACTGTGGGG CTGACTTCCC AGGAGTCCCA CTAGCACCCC CACGGCACTG GGATGATGTA
GTCTGTGAGT CCCGCTACTC AGAGATTGAG CCATCCACTG AAGGCGAGGT CATCTATCGT
GTGCTGGACC CTGCCATCCC TATCCCAGAC CCCTACAGCT CACGGATTCA GAACCTGTTG
AAGATCACCA ACCTACGGGT GAACCTGACT CGTCTACACA CGTTGGGAGA CAACCTACTC
GACCCACGGA GGGAGATCCG AGAGAAGTAC TACTATGCCC TCTATGAGCT GGTTGTACGT
GGCAACTGCT TCTGCTACGG ACACGCCTCA GAGTGTGCAC CCGCCCCAGG GGCACCAGCC
CATGCTGAGG GCATGGTGCA CGGAGCTTGC ATCTGCAAAC ACAACACACG TGGCCTCAAC
TGCGAGCAGT GTCAGGATTT CTATCGTGAC CTGCCCTGGC GTCCGGCTGA GGACGGCCAT
AGTCATGCCT GTAGGAAGTG TGAGTGCCAT GGGCACACCC ACAGCTGCCA CTTCGACATG
GCCGTATACC TGGCATCTGG CAATGTGAGT GGAGGTGTGT GTGATGGATG TCAGCATAAC
ACAGCTGGGC GCCACTGTGA GCTCTGTCGG CCCTTCTTCT ACCGTGACCC AACCAAGGAC
CTGCGGGATC CGGCTGTGTG CCGCTCCTGT GATTGTGACC CCATGGGTTC TCAAGACGGT
GGTCGCTGTG ATTCCCATGA TGACCCTGCA CTGGGACTGG TCTCCGGCCA GTGTCGCTGC
AAAGAACATG TGGTGGGCAC TCGCTGCCAG CAATGCCGTG ATGGCTTCTT TGGGCTCAGC
ATCAGTGACC GTCTGGGCTG CCGGCGATGT CAATGTAATG CACGGGGCAC AGTGCCTGGG
AGCACTCCTT GTGACCCCAA CAGTGGATCC TGTTACTGCA AACGTCTAGT GACTGGACGT
GGATGTGACC GCTGCCTGCC TGGCCACTGG GGCCTGAGCC ACGACCTGCT CGGCTGCCGC
CCCTGTGACT GCGACGTGGG TGGTGCTTTG GATCCCCAGT GTGATGAGGG CACAGGTCAA
TGCCACTGCC GCCAGCACAT GGTTGGGCGA CGCTGTGAGC AGGTGCAACC TGGCTACTTC
CGGCCCTTCC TGGACCACCT AATTTGGGAG GCTGAGGACA CCCGAGGGCA GGTGCTCGAT
GTGGTGGAGC GCCTGGTGAC CCCCGGGGAA ACTCCATCCT GGACTGGCTC AGGCTTCGTG
CGGCTACAGG AAGGTCAGAC CCTGGAGTTC CTGGTGGCCT CTGTGCCGAA GGCTATGGAC
TATGACCTGC TGCTGCGCTT AGAGCCCCAG GTCCCTGAGC AATGGGCAGA GTTGGAACTG
ATTGTGCAGC GTCCAGGGCC TGTGCCTGCC CACAGCCTGT GTGGGCATTT GGTGCCCAAG
GATGATCGCA TCCAAGGGAC TCTGCAACCA CATGCCAGGT ACTTGATATT TCCTAATCCT
GTCTGCCTTG AGCCTGGTAT CTCCTACAAG CTGCATCTGA AGCTGGTACG GACAGGGGGA
AGTGCCCAGC CTGAGACTCC CTACTCTGGA CCTGGCCTGC TCATTGACTC GCTGGTGCTG
CTGCCCCGTG TCCTGGTGCT AGAGATGTTT AGTGGGGGTG ATGCTGCTGC CCTGGAGCGC
CAGGCCACCT TTGAACGCTA CCAATGCCAT GAGGAGGGTC TGGTGCCCAG CAAGACTTCT
CCCTCTGAGG CCTGCGCACC CCTCCTCATC AGCCTGTCCA CCCTCATCTA CAATGGTGCC
CTGCCATGTC AGTGCAACCC TCAAGGTTCA CTGAGTTCTG AGTGCAACCC TCATGGTGGT
CAGTGCCTGT GCAAGCCTGG AGTGGTTGGG CGCCGCTGTG ACCTCTGTGC CCCTGGCTAC
TATGGCTTTG GCCCCACAGG CTGTCAAGCC TGCCAGTGCA GCCACGAGGG GGCACTCAGC
AGTCTCTGTG AAAAGACCAG TGGGCAATGT CTCTGTCGAA CTGGTGCCTT TGGGCTTCGC
TGTGACCGCT GCCAGCGTGG CCAGTGGGGA TTCCCTAGCT GCCGGCCATG TGTCTGCAAT
GGGCATGCAG ATGAGTGCAA CACCCACACA GGCGCTTGCC TGGGCTGCCG TGATCACACA
GGGGGTGAGC ACTGTGAAAG GTGCATTGCT GGTTTCCACG GGGACCCACG GCTGCCATAT
GGGGGCCAGT GCCGGCCCTG TCCCTGTCCT GAAGGCCCTG GGAGCCAACG GCACTTTGCT
ACTTCTTGCC ACCAGGATGA ATATTCCCAG CAGATTGTGT GCCACTGCCG GGCAGGCTAT
ACGGGGCTGC GATGTGAAGC TTGTGCCCCT GGGCACTTTG GGGACCCATC AAGGCCAGGT
GGCCGGTGCC AACTGTGTGA GTGCAGTGGG AACATTGACC CAATGGATCC TGATGCCTGT
GACCCCCACA CGGGGCAATG CCTGCGCTGT TTACACCACA CAGAGGGTCC ACACTGTGCC
CACTGCAAGC CTGGCTTCCA TGGGCAGGCT GCCCGACAGA GCTGTCACCG CTGCACATGC
AACCTGCTGG GCACAAATCC GCAGCAGTGC CCATCTCCTG ACCAGTGCCA CTGTGATCCA
AGCAGTGGGC AGTGCCCATG CCTCCCCAAT GTCCAGGGCC CTAGCTGTGA CCGCTGTGCC
CCCAACTTCT GGAACCTCAC CAGTGGCCAT GGTTGCCAGC CTTGTGCCTG CCACCCAAGC
CGGGCCAGAG GCCCCACCTG CAACGAGTTC ACAGGGCAGT GCCACTGCCG TGCCGGCTTT
GGAGGGCGGA CTTGTTCTGA GTGCCAAGAG CTCCACTGGG GAGACCCTGG GTTGCAGTGC
CATGCCTGTG ATTGTGACTC TCGTGGAATA GATACACCTC AGTGTCACCG CTTCACAGGT
CACTGCAGCT GCCGCCCAGG GGTGTCTGGT GTGCGCTGTG ACCAGTGTGC CCGTGGCTTC
TCAGGAATCT TTCCTGCCTG CCATCCCTGC CATGCATGCT TCGGGGATTG GGACCGAGTG
GTGCAGGACT TGGCAGCCCG TACACAGCGC CTAGAGCAGC GGGCGCAGGA GTTGCAACAG
ACGGGTGTGC TGGGTGCCTT TGAGAGCAGC TTCTGGCACA TGCAGGAGAA GCTGGGCATT
GTGCAGGGCA TCGTAGGTGC CCGCAACACC TCAGCCGCCT CCACTGCACA GCTTGTGGAG
GCCACAGAGG AGCTGCGGCG TGAAATTGGG GAGGCCACTG AGCACCTGAC TCAGCTCGAG
GCAGACCTGA CAGATGTGCA AGATGAGAAC TTCAATGCCA ACCATGCACT AAGTGGTCTG
GAGCGAGATA GGCTTGCACT TAATCTCACA CTGCGGCAGC TCGACCAGCA TCTTGACTTG
CTCAAACATT CAAACTTCCT GGGTGCCTAT GACAGCATCC GGCATGCCCA TAGCCAGTCT
GCAGAGGCAG AACGTCGTGC CAATACCTCA GCCCTGGCAG TACCTAGCCC TGTGAGCAAC
TCGGCAAGTG CTCGGCATCG GACAGAGGCA CTGATGGATG CTCAGAAGGA GGACTTCAAC
AGCAAACACA TGGCCAACCA GCGGGCACTT GGCAAGCTCT CTGCCCATAC CCACACCCTG
AGCCTGACAG ACATAAATGA GCTGGTGTGT GGGGCACCAG GGGATGCACC CTGTGCTACA
AGCCCTTGTG GGGGTGCCGG CTGTCGAGAT GAGGATGGGC AGCCGCGCTG TGGGGGCCTC
AGCTGCAATG GGGCAGCGGC TACAGCAGAC CTAGCACTGG GCCGGGCCCG GCACACACAG
GCAGAGCTGC AGCGGGCACT GGCAGAAGGT GGTAGCATCC TCAGCAGAGT GGCTGAGACT
CGTCGGCAGG CAAGCGAGGC ACAGCAGCGG GCCCAGGCAG CCCTGGACAA GGCTAATGCT
TCCAGGGGAC AGGTGGAACA GGCCAACCAG GAACTTCAAG AACTTATCCA GAGTGTGAAG
GACTTCCTCA ACCAGGAGGG GGCTGATCCT GATAGCATTG AAATGGTGGC CACACGGGTG
CTAGAGCTCT CCATCCCAGC TTCAGCTGAG CAGATCCAGC ACCTGGCGGG TGCGATTGCA
GAGCGAGTCC GGAGCCTGGC AGATGTGGAT GCGATCCTGG CACGTACTGT AGGAGATGTG
CGTCGTGCCG AGCAGCTACT GCAGGATGCA CGGCGGGCAA GGAGCTGGGC TGAGGATGAG
AAACAGAAGG CAGAGACAGT ACAGGCAGCA CTGGAGGAGG CCCAGCGGGC ACAGGGTATT
GCCCAGGGTG CCATCCGGGG GGCAGTGGCT GACACACGGG ACACAGAGCA GACCCTGTAC
CAGGTACAGG AGAGGATGGC AGGTGCAGAG CGGGCACTGA GCTCTGCAGG TGAAAGGGCT
CGGCAGTTGG ATGCTCTCCT GGAGGCTCTG AAATTGAAAC GGGCAGGAAA TAGTCTGGCA
GCCTCTACAG CAGAAGAAAC GGCAGGCAGT GCCCAGGGTC GTGCCCAGGA GGCTGAGCAG
CTGCTACGCG GTCCTCTGGG TGATCAGTAC CAGACGGTGA AGGCCCTAGC TGAGCGCAAG
GCCCAAGGTG TGCTGGCTGC ACAGGCAAGG GCAGAACAAC TGCGGGATGA GGCTCGGGAC
CTGTTGCAAG CCGCTCAGGA CAAGCTGCAG CGGCTACAGG AATTGGAAGG CACCTATGAG
GAAAATGAGC GGGCACTGGA GAGTAAGGCA GCCCAGTTGG ACGGGTTGGA GGCCAGGATG
CGCAGCGTGC TTCAAGCCAT CAACTTGCAG GTGCAGATCT ACAACACCTG CCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu17891
Clone ID Related Accession (Same CDS sequence) XM_005265127.3 , NM_002292.3
Accession Version XM_005265127.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5397bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product laminin subunit beta-2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005265127.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
ATGGAGCTGA CCTCAAGGGA AAGAGGGAGG GGACAGCCTC TGCCCTGGGA ACTTCGACTG 
GGCCTACTGC TAAGCGTGCT GGCTGCCACA CTGGCACAGG CCCCTGCCCC GGATGTGCCT
GGCTGTTCCA GGGGAAGCTG CTACCCCGCC ACGGGCGACC TGCTGGTGGG CCGAGCTGAC
AGACTGACTG CCTCATCCAC TTGTGGCCTG AATGGCCCCC AGCCCTACTG CATCGTCAGT
CACCTGCAGG ACGAAAAGAA GTGCTTCCTT TGTGACTCCC GGCGCCCCTT CTCTGCTAGA
GACAACCCAC ACAGCCATCG CATCCAGAAT GTAGTCACCA GCTTTGCACC ACAGCGGCGG
GCAGCCTGGT GGCAGTCAGA GAATGGTATC CCTGCGGTCA CCATCCAGCT GGACCTGGAG
GCTGAGTTTC ATTTCACACA CCTCATTATG ACCTTCAAGA CATTTCGCCC TGCTGCCATG
CTGGTGGAAC GCTCAGCAGA CTTTGGCCGC ACCTGGCATG TGTACCGATA TTTCTCCTAT
GACTGTGGGG CTGACTTCCC AGGAGTCCCA CTAGCACCCC CACGGCACTG GGATGATGTA
GTCTGTGAGT CCCGCTACTC AGAGATTGAG CCATCCACTG AAGGCGAGGT CATCTATCGT
GTGCTGGACC CTGCCATCCC TATCCCAGAC CCCTACAGCT CACGGATTCA GAACCTGTTG
AAGATCACCA ACCTACGGGT GAACCTGACT CGTCTACACA CGTTGGGAGA CAACCTACTC
GACCCACGGA GGGAGATCCG AGAGAAGTAC TACTATGCCC TCTATGAGCT GGTTGTACGT
GGCAACTGCT TCTGCTACGG ACACGCCTCA GAGTGTGCAC CCGCCCCAGG GGCACCAGCC
CATGCTGAGG GCATGGTGCA CGGAGCTTGC ATCTGCAAAC ACAACACACG TGGCCTCAAC
TGCGAGCAGT GTCAGGATTT CTATCGTGAC CTGCCCTGGC GTCCGGCTGA GGACGGCCAT
AGTCATGCCT GTAGGAAGTG TGAGTGCCAT GGGCACACCC ACAGCTGCCA CTTCGACATG
GCCGTATACC TGGCATCTGG CAATGTGAGT GGAGGTGTGT GTGATGGATG TCAGCATAAC
ACAGCTGGGC GCCACTGTGA GCTCTGTCGG CCCTTCTTCT ACCGTGACCC AACCAAGGAC
CTGCGGGATC CGGCTGTGTG CCGCTCCTGT GATTGTGACC CCATGGGTTC TCAAGACGGT
GGTCGCTGTG ATTCCCATGA TGACCCTGCA CTGGGACTGG TCTCCGGCCA GTGTCGCTGC
AAAGAACATG TGGTGGGCAC TCGCTGCCAG CAATGCCGTG ATGGCTTCTT TGGGCTCAGC
ATCAGTGACC GTCTGGGCTG CCGGCGATGT CAATGTAATG CACGGGGCAC AGTGCCTGGG
AGCACTCCTT GTGACCCCAA CAGTGGATCC TGTTACTGCA AACGTCTAGT GACTGGACGT
GGATGTGACC GCTGCCTGCC TGGCCACTGG GGCCTGAGCC ACGACCTGCT CGGCTGCCGC
CCCTGTGACT GCGACGTGGG TGGTGCTTTG GATCCCCAGT GTGATGAGGG CACAGGTCAA
TGCCACTGCC GCCAGCACAT GGTTGGGCGA CGCTGTGAGC AGGTGCAACC TGGCTACTTC
CGGCCCTTCC TGGACCACCT AATTTGGGAG GCTGAGGACA CCCGAGGGCA GGTGCTCGAT
GTGGTGGAGC GCCTGGTGAC CCCCGGGGAA ACTCCATCCT GGACTGGCTC AGGCTTCGTG
CGGCTACAGG AAGGTCAGAC CCTGGAGTTC CTGGTGGCCT CTGTGCCGAA GGCTATGGAC
TATGACCTGC TGCTGCGCTT AGAGCCCCAG GTCCCTGAGC AATGGGCAGA GTTGGAACTG
ATTGTGCAGC GTCCAGGGCC TGTGCCTGCC CACAGCCTGT GTGGGCATTT GGTGCCCAAG
GATGATCGCA TCCAAGGGAC TCTGCAACCA CATGCCAGGT ACTTGATATT TCCTAATCCT
GTCTGCCTTG AGCCTGGTAT CTCCTACAAG CTGCATCTGA AGCTGGTACG GACAGGGGGA
AGTGCCCAGC CTGAGACTCC CTACTCTGGA CCTGGCCTGC TCATTGACTC GCTGGTGCTG
CTGCCCCGTG TCCTGGTGCT AGAGATGTTT AGTGGGGGTG ATGCTGCTGC CCTGGAGCGC
CAGGCCACCT TTGAACGCTA CCAATGCCAT GAGGAGGGTC TGGTGCCCAG CAAGACTTCT
CCCTCTGAGG CCTGCGCACC CCTCCTCATC AGCCTGTCCA CCCTCATCTA CAATGGTGCC
CTGCCATGTC AGTGCAACCC TCAAGGTTCA CTGAGTTCTG AGTGCAACCC TCATGGTGGT
CAGTGCCTGT GCAAGCCTGG AGTGGTTGGG CGCCGCTGTG ACCTCTGTGC CCCTGGCTAC
TATGGCTTTG GCCCCACAGG CTGTCAAGCC TGCCAGTGCA GCCACGAGGG GGCACTCAGC
AGTCTCTGTG AAAAGACCAG TGGGCAATGT CTCTGTCGAA CTGGTGCCTT TGGGCTTCGC
TGTGACCGCT GCCAGCGTGG CCAGTGGGGA TTCCCTAGCT GCCGGCCATG TGTCTGCAAT
GGGCATGCAG ATGAGTGCAA CACCCACACA GGCGCTTGCC TGGGCTGCCG TGATCACACA
GGGGGTGAGC ACTGTGAAAG GTGCATTGCT GGTTTCCACG GGGACCCACG GCTGCCATAT
GGGGGCCAGT GCCGGCCCTG TCCCTGTCCT GAAGGCCCTG GGAGCCAACG GCACTTTGCT
ACTTCTTGCC ACCAGGATGA ATATTCCCAG CAGATTGTGT GCCACTGCCG GGCAGGCTAT
ACGGGGCTGC GATGTGAAGC TTGTGCCCCT GGGCACTTTG GGGACCCATC AAGGCCAGGT
GGCCGGTGCC AACTGTGTGA GTGCAGTGGG AACATTGACC CAATGGATCC TGATGCCTGT
GACCCCCACA CGGGGCAATG CCTGCGCTGT TTACACCACA CAGAGGGTCC ACACTGTGCC
CACTGCAAGC CTGGCTTCCA TGGGCAGGCT GCCCGACAGA GCTGTCACCG CTGCACATGC
AACCTGCTGG GCACAAATCC GCAGCAGTGC CCATCTCCTG ACCAGTGCCA CTGTGATCCA
AGCAGTGGGC AGTGCCCATG CCTCCCCAAT GTCCAGGGCC CTAGCTGTGA CCGCTGTGCC
CCCAACTTCT GGAACCTCAC CAGTGGCCAT GGTTGCCAGC CTTGTGCCTG CCACCCAAGC
CGGGCCAGAG GCCCCACCTG CAACGAGTTC ACAGGGCAGT GCCACTGCCG TGCCGGCTTT
GGAGGGCGGA CTTGTTCTGA GTGCCAAGAG CTCCACTGGG GAGACCCTGG GTTGCAGTGC
CATGCCTGTG ATTGTGACTC TCGTGGAATA GATACACCTC AGTGTCACCG CTTCACAGGT
CACTGCAGCT GCCGCCCAGG GGTGTCTGGT GTGCGCTGTG ACCAGTGTGC CCGTGGCTTC
TCAGGAATCT TTCCTGCCTG CCATCCCTGC CATGCATGCT TCGGGGATTG GGACCGAGTG
GTGCAGGACT TGGCAGCCCG TACACAGCGC CTAGAGCAGC GGGCGCAGGA GTTGCAACAG
ACGGGTGTGC TGGGTGCCTT TGAGAGCAGC TTCTGGCACA TGCAGGAGAA GCTGGGCATT
GTGCAGGGCA TCGTAGGTGC CCGCAACACC TCAGCCGCCT CCACTGCACA GCTTGTGGAG
GCCACAGAGG AGCTGCGGCG TGAAATTGGG GAGGCCACTG AGCACCTGAC TCAGCTCGAG
GCAGACCTGA CAGATGTGCA AGATGAGAAC TTCAATGCCA ACCATGCACT AAGTGGTCTG
GAGCGAGATA GGCTTGCACT TAATCTCACA CTGCGGCAGC TCGACCAGCA TCTTGACTTG
CTCAAACATT CAAACTTCCT GGGTGCCTAT GACAGCATCC GGCATGCCCA TAGCCAGTCT
GCAGAGGCAG AACGTCGTGC CAATACCTCA GCCCTGGCAG TACCTAGCCC TGTGAGCAAC
TCGGCAAGTG CTCGGCATCG GACAGAGGCA CTGATGGATG CTCAGAAGGA GGACTTCAAC
AGCAAACACA TGGCCAACCA GCGGGCACTT GGCAAGCTCT CTGCCCATAC CCACACCCTG
AGCCTGACAG ACATAAATGA GCTGGTGTGT GGGGCACCAG GGGATGCACC CTGTGCTACA
AGCCCTTGTG GGGGTGCCGG CTGTCGAGAT GAGGATGGGC AGCCGCGCTG TGGGGGCCTC
AGCTGCAATG GGGCAGCGGC TACAGCAGAC CTAGCACTGG GCCGGGCCCG GCACACACAG
GCAGAGCTGC AGCGGGCACT GGCAGAAGGT GGTAGCATCC TCAGCAGAGT GGCTGAGACT
CGTCGGCAGG CAAGCGAGGC ACAGCAGCGG GCCCAGGCAG CCCTGGACAA GGCTAATGCT
TCCAGGGGAC AGGTGGAACA GGCCAACCAG GAACTTCAAG AACTTATCCA GAGTGTGAAG
GACTTCCTCA ACCAGGAGGG GGCTGATCCT GATAGCATTG AAATGGTGGC CACACGGGTG
CTAGAGCTCT CCATCCCAGC TTCAGCTGAG CAGATCCAGC ACCTGGCGGG TGCGATTGCA
GAGCGAGTCC GGAGCCTGGC AGATGTGGAT GCGATCCTGG CACGTACTGT AGGAGATGTG
CGTCGTGCCG AGCAGCTACT GCAGGATGCA CGGCGGGCAA GGAGCTGGGC TGAGGATGAG
AAACAGAAGG CAGAGACAGT ACAGGCAGCA CTGGAGGAGG CCCAGCGGGC ACAGGGTATT
GCCCAGGGTG CCATCCGGGG GGCAGTGGCT GACACACGGG ACACAGAGCA GACCCTGTAC
CAGGTACAGG AGAGGATGGC AGGTGCAGAG CGGGCACTGA GCTCTGCAGG TGAAAGGGCT
CGGCAGTTGG ATGCTCTCCT GGAGGCTCTG AAATTGAAAC GGGCAGGAAA TAGTCTGGCA
GCCTCTACAG CAGAAGAAAC GGCAGGCAGT GCCCAGGGTC GTGCCCAGGA GGCTGAGCAG
CTGCTACGCG GTCCTCTGGG TGATCAGTAC CAGACGGTGA AGGCCCTAGC TGAGCGCAAG
GCCCAAGGTG TGCTGGCTGC ACAGGCAAGG GCAGAACAAC TGCGGGATGA GGCTCGGGAC
CTGTTGCAAG CCGCTCAGGA CAAGCTGCAG CGGCTACAGG AATTGGAAGG CACCTATGAG
GAAAATGAGC GGGCACTGGA GAGTAAGGCA GCCCAGTTGG ACGGGTTGGA GGCCAGGATG
CGCAGCGTGC TTCAAGCCAT CAACTTGCAG GTGCAGATCT ACAACACCTG CCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005265184.1
CDS99..5495
Misc Feature(1)267..941
Misc Feature(2)945..1103
Misc Feature(3)order(945..947,951..953,972..974,1026..1028,1032..1034,1059..1061)
Misc Feature(4)1137..1295
Misc Feature(5)order(1137..1139,1143..1145,1164..1166,1218..1220,1227..1229,1254..1256)
Misc Feature(6)1326..1499
Misc Feature(7)order(1326..1328,1332..1334,1365..1367,1410..1412,1416..1418,1443..1445)
Misc Feature(8)1503..1658
Misc Feature(9)order(1506..1508,1512..1514,1548..1550,1569..1571,1575..1577,1602..1604)
Misc Feature(10)1659..1784
Misc Feature(11)order(1662..1664,1668..1670,1698..1700,1719..1721,1725..1727,1752..1754)
Misc Feature(12)2445..2591
Misc Feature(13)order(2445..2447,2451..2453,2481..2483,2502..2504,2508..2510,2535..2537)
Misc Feature(14)2589..2735
Misc Feature(15)order(2589..2591,2595..2597,2625..2627,2646..2648,2652..2654,2679..2681)
Misc Feature(16)2724..2861
Misc Feature(17)order(2727..2729,2733..2735,2754..2756,2775..2777,2784..2786,2811..2813)
Misc Feature(18)2874..3050
Misc Feature(19)order(2877..2879,2883..2885,2925..2927,2958..2960,2964..2966,2991..2993)
Misc Feature(20)3054..>3182
Misc Feature(21)order(3054..3056,3060..3062,3096..3098,3117..3119,3126..3128,3153..3155)
Misc Feature(22)3207..3377
Misc Feature(23)order(3210..3212,3216..3218,3246..3248,3291..3293,3297..3299,3324..3326)
Misc Feature(24)3381..3527
Misc Feature(25)order(3381..3383,3387..3389,3417..3419,3438..3440,3444..3446,3471..3473)
Misc Feature(26)3525..>3644
Misc Feature(27)order(3525..3527,3531..3533,3561..3563,3582..3584,3588..3590,3615..3617)
Misc Feature(28)4431..5201
Misc Feature(29)<4455..4721
Misc Feature(30)4545..5108
Misc Feature(31)5040..5363
Misc Feature(32)<5136..5474
Translation

Target ORF information:

RefSeq Version XM_005265127.3
Organism Homo sapiens(Human)
Definition Homo sapiens laminin subunit beta 2 (LAMB2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005265127.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
ATGGAGCTGA CCTCAAGGGA AAGAGGGAGG GGACAGCCTC TGCCCTGGGA ACTTCGACTG 
GGCCTACTGC TAAGCGTGCT GGCTGCCACA CTGGCACAGG CCCCTGCCCC GGATGTGCCT
GGCTGTTCCA GGGGAAGCTG CTACCCCGCC ACGGGCGACC TGCTGGTGGG CCGAGCTGAC
AGACTGACTG CCTCATCCAC TTGTGGCCTG AATGGCCCCC AGCCCTACTG CATCGTCAGT
CACCTGCAGG ACGAAAAGAA GTGCTTCCTT TGTGACTCCC GGCGCCCCTT CTCTGCTAGA
GACAACCCAC ACAGCCATCG CATCCAGAAT GTAGTCACCA GCTTTGCACC ACAGCGGCGG
GCAGCCTGGT GGCAGTCAGA GAATGGTATC CCTGCGGTCA CCATCCAGCT GGACCTGGAG
GCTGAGTTTC ATTTCACACA CCTCATTATG ACCTTCAAGA CATTTCGCCC TGCTGCCATG
CTGGTGGAAC GCTCAGCAGA CTTTGGCCGC ACCTGGCATG TGTACCGATA TTTCTCCTAT
GACTGTGGGG CTGACTTCCC AGGAGTCCCA CTAGCACCCC CACGGCACTG GGATGATGTA
GTCTGTGAGT CCCGCTACTC AGAGATTGAG CCATCCACTG AAGGCGAGGT CATCTATCGT
GTGCTGGACC CTGCCATCCC TATCCCAGAC CCCTACAGCT CACGGATTCA GAACCTGTTG
AAGATCACCA ACCTACGGGT GAACCTGACT CGTCTACACA CGTTGGGAGA CAACCTACTC
GACCCACGGA GGGAGATCCG AGAGAAGTAC TACTATGCCC TCTATGAGCT GGTTGTACGT
GGCAACTGCT TCTGCTACGG ACACGCCTCA GAGTGTGCAC CCGCCCCAGG GGCACCAGCC
CATGCTGAGG GCATGGTGCA CGGAGCTTGC ATCTGCAAAC ACAACACACG TGGCCTCAAC
TGCGAGCAGT GTCAGGATTT CTATCGTGAC CTGCCCTGGC GTCCGGCTGA GGACGGCCAT
AGTCATGCCT GTAGGAAGTG TGAGTGCCAT GGGCACACCC ACAGCTGCCA CTTCGACATG
GCCGTATACC TGGCATCTGG CAATGTGAGT GGAGGTGTGT GTGATGGATG TCAGCATAAC
ACAGCTGGGC GCCACTGTGA GCTCTGTCGG CCCTTCTTCT ACCGTGACCC AACCAAGGAC
CTGCGGGATC CGGCTGTGTG CCGCTCCTGT GATTGTGACC CCATGGGTTC TCAAGACGGT
GGTCGCTGTG ATTCCCATGA TGACCCTGCA CTGGGACTGG TCTCCGGCCA GTGTCGCTGC
AAAGAACATG TGGTGGGCAC TCGCTGCCAG CAATGCCGTG ATGGCTTCTT TGGGCTCAGC
ATCAGTGACC GTCTGGGCTG CCGGCGATGT CAATGTAATG CACGGGGCAC AGTGCCTGGG
AGCACTCCTT GTGACCCCAA CAGTGGATCC TGTTACTGCA AACGTCTAGT GACTGGACGT
GGATGTGACC GCTGCCTGCC TGGCCACTGG GGCCTGAGCC ACGACCTGCT CGGCTGCCGC
CCCTGTGACT GCGACGTGGG TGGTGCTTTG GATCCCCAGT GTGATGAGGG CACAGGTCAA
TGCCACTGCC GCCAGCACAT GGTTGGGCGA CGCTGTGAGC AGGTGCAACC TGGCTACTTC
CGGCCCTTCC TGGACCACCT AATTTGGGAG GCTGAGGACA CCCGAGGGCA GGTGCTCGAT
GTGGTGGAGC GCCTGGTGAC CCCCGGGGAA ACTCCATCCT GGACTGGCTC AGGCTTCGTG
CGGCTACAGG AAGGTCAGAC CCTGGAGTTC CTGGTGGCCT CTGTGCCGAA GGCTATGGAC
TATGACCTGC TGCTGCGCTT AGAGCCCCAG GTCCCTGAGC AATGGGCAGA GTTGGAACTG
ATTGTGCAGC GTCCAGGGCC TGTGCCTGCC CACAGCCTGT GTGGGCATTT GGTGCCCAAG
GATGATCGCA TCCAAGGGAC TCTGCAACCA CATGCCAGGT ACTTGATATT TCCTAATCCT
GTCTGCCTTG AGCCTGGTAT CTCCTACAAG CTGCATCTGA AGCTGGTACG GACAGGGGGA
AGTGCCCAGC CTGAGACTCC CTACTCTGGA CCTGGCCTGC TCATTGACTC GCTGGTGCTG
CTGCCCCGTG TCCTGGTGCT AGAGATGTTT AGTGGGGGTG ATGCTGCTGC CCTGGAGCGC
CAGGCCACCT TTGAACGCTA CCAATGCCAT GAGGAGGGTC TGGTGCCCAG CAAGACTTCT
CCCTCTGAGG CCTGCGCACC CCTCCTCATC AGCCTGTCCA CCCTCATCTA CAATGGTGCC
CTGCCATGTC AGTGCAACCC TCAAGGTTCA CTGAGTTCTG AGTGCAACCC TCATGGTGGT
CAGTGCCTGT GCAAGCCTGG AGTGGTTGGG CGCCGCTGTG ACCTCTGTGC CCCTGGCTAC
TATGGCTTTG GCCCCACAGG CTGTCAAGCC TGCCAGTGCA GCCACGAGGG GGCACTCAGC
AGTCTCTGTG AAAAGACCAG TGGGCAATGT CTCTGTCGAA CTGGTGCCTT TGGGCTTCGC
TGTGACCGCT GCCAGCGTGG CCAGTGGGGA TTCCCTAGCT GCCGGCCATG TGTCTGCAAT
GGGCATGCAG ATGAGTGCAA CACCCACACA GGCGCTTGCC TGGGCTGCCG TGATCACACA
GGGGGTGAGC ACTGTGAAAG GTGCATTGCT GGTTTCCACG GGGACCCACG GCTGCCATAT
GGGGGCCAGT GCCGGCCCTG TCCCTGTCCT GAAGGCCCTG GGAGCCAACG GCACTTTGCT
ACTTCTTGCC ACCAGGATGA ATATTCCCAG CAGATTGTGT GCCACTGCCG GGCAGGCTAT
ACGGGGCTGC GATGTGAAGC TTGTGCCCCT GGGCACTTTG GGGACCCATC AAGGCCAGGT
GGCCGGTGCC AACTGTGTGA GTGCAGTGGG AACATTGACC CAATGGATCC TGATGCCTGT
GACCCCCACA CGGGGCAATG CCTGCGCTGT TTACACCACA CAGAGGGTCC ACACTGTGCC
CACTGCAAGC CTGGCTTCCA TGGGCAGGCT GCCCGACAGA GCTGTCACCG CTGCACATGC
AACCTGCTGG GCACAAATCC GCAGCAGTGC CCATCTCCTG ACCAGTGCCA CTGTGATCCA
AGCAGTGGGC AGTGCCCATG CCTCCCCAAT GTCCAGGGCC CTAGCTGTGA CCGCTGTGCC
CCCAACTTCT GGAACCTCAC CAGTGGCCAT GGTTGCCAGC CTTGTGCCTG CCACCCAAGC
CGGGCCAGAG GCCCCACCTG CAACGAGTTC ACAGGGCAGT GCCACTGCCG TGCCGGCTTT
GGAGGGCGGA CTTGTTCTGA GTGCCAAGAG CTCCACTGGG GAGACCCTGG GTTGCAGTGC
CATGCCTGTG ATTGTGACTC TCGTGGAATA GATACACCTC AGTGTCACCG CTTCACAGGT
CACTGCAGCT GCCGCCCAGG GGTGTCTGGT GTGCGCTGTG ACCAGTGTGC CCGTGGCTTC
TCAGGAATCT TTCCTGCCTG CCATCCCTGC CATGCATGCT TCGGGGATTG GGACCGAGTG
GTGCAGGACT TGGCAGCCCG TACACAGCGC CTAGAGCAGC GGGCGCAGGA GTTGCAACAG
ACGGGTGTGC TGGGTGCCTT TGAGAGCAGC TTCTGGCACA TGCAGGAGAA GCTGGGCATT
GTGCAGGGCA TCGTAGGTGC CCGCAACACC TCAGCCGCCT CCACTGCACA GCTTGTGGAG
GCCACAGAGG AGCTGCGGCG TGAAATTGGG GAGGCCACTG AGCACCTGAC TCAGCTCGAG
GCAGACCTGA CAGATGTGCA AGATGAGAAC TTCAATGCCA ACCATGCACT AAGTGGTCTG
GAGCGAGATA GGCTTGCACT TAATCTCACA CTGCGGCAGC TCGACCAGCA TCTTGACTTG
CTCAAACATT CAAACTTCCT GGGTGCCTAT GACAGCATCC GGCATGCCCA TAGCCAGTCT
GCAGAGGCAG AACGTCGTGC CAATACCTCA GCCCTGGCAG TACCTAGCCC TGTGAGCAAC
TCGGCAAGTG CTCGGCATCG GACAGAGGCA CTGATGGATG CTCAGAAGGA GGACTTCAAC
AGCAAACACA TGGCCAACCA GCGGGCACTT GGCAAGCTCT CTGCCCATAC CCACACCCTG
AGCCTGACAG ACATAAATGA GCTGGTGTGT GGGGCACCAG GGGATGCACC CTGTGCTACA
AGCCCTTGTG GGGGTGCCGG CTGTCGAGAT GAGGATGGGC AGCCGCGCTG TGGGGGCCTC
AGCTGCAATG GGGCAGCGGC TACAGCAGAC CTAGCACTGG GCCGGGCCCG GCACACACAG
GCAGAGCTGC AGCGGGCACT GGCAGAAGGT GGTAGCATCC TCAGCAGAGT GGCTGAGACT
CGTCGGCAGG CAAGCGAGGC ACAGCAGCGG GCCCAGGCAG CCCTGGACAA GGCTAATGCT
TCCAGGGGAC AGGTGGAACA GGCCAACCAG GAACTTCAAG AACTTATCCA GAGTGTGAAG
GACTTCCTCA ACCAGGAGGG GGCTGATCCT GATAGCATTG AAATGGTGGC CACACGGGTG
CTAGAGCTCT CCATCCCAGC TTCAGCTGAG CAGATCCAGC ACCTGGCGGG TGCGATTGCA
GAGCGAGTCC GGAGCCTGGC AGATGTGGAT GCGATCCTGG CACGTACTGT AGGAGATGTG
CGTCGTGCCG AGCAGCTACT GCAGGATGCA CGGCGGGCAA GGAGCTGGGC TGAGGATGAG
AAACAGAAGG CAGAGACAGT ACAGGCAGCA CTGGAGGAGG CCCAGCGGGC ACAGGGTATT
GCCCAGGGTG CCATCCGGGG GGCAGTGGCT GACACACGGG ACACAGAGCA GACCCTGTAC
CAGGTACAGG AGAGGATGGC AGGTGCAGAG CGGGCACTGA GCTCTGCAGG TGAAAGGGCT
CGGCAGTTGG ATGCTCTCCT GGAGGCTCTG AAATTGAAAC GGGCAGGAAA TAGTCTGGCA
GCCTCTACAG CAGAAGAAAC GGCAGGCAGT GCCCAGGGTC GTGCCCAGGA GGCTGAGCAG
CTGCTACGCG GTCCTCTGGG TGATCAGTAC CAGACGGTGA AGGCCCTAGC TGAGCGCAAG
GCCCAAGGTG TGCTGGCTGC ACAGGCAAGG GCAGAACAAC TGCGGGATGA GGCTCGGGAC
CTGTTGCAAG CCGCTCAGGA CAAGCTGCAG CGGCTACAGG AATTGGAAGG CACCTATGAG
GAAAATGAGC GGGCACTGGA GAGTAAGGCA GCCCAGTTGG ACGGGTTGGA GGCCAGGATG
CGCAGCGTGC TTCAAGCCAT CAACTTGCAG GTGCAGATCT ACAACACCTG CCAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

LAMB2 mutation with different phenotypes in China
.
Clinical nephrology87 (2017)(1)33-38(2017 Jan)
Zhang H,Cui J,Wang F,Xiao H,Ding J,Yao Y


book

Laminins 411 and 421 differentially promote tumor cell migration via α6β1 integrin and MCAM (CD146).
Matrix biology : journal of the International Society for Matrix Biology3869-83(2014 Sep)
Ishikawa T,Wondimu Z,Oikawa Y,Gentilcore G,Kiessling R,Egyhazi Brage S,Hansson J,Patarroyo M


book

Retrospective mutational analysis of NPHS1, NPHS2, WT1 and LAMB2 in children with steroid-resistant focal segmental glomerulosclerosis - a single-centre experience.
Bosnian journal of basic medical sciences14(2)89-93(2014 May)
Bińczak-Kuleta A,Rubik J,Litwin M,Ryder M,Lewandowska K,Taryma-Leśniak O,Clark JS,Grenda R,Ciechanowicz A


book

Proteomics analysis of cardiac extracellular matrix remodeling in a porcine model of ischemia/reperfusion injury.
Circulation125(6)789-802(2012 Feb)
Barallobre-Barreiro J,Didangelos A,Schoendube FA,Drozdov I,Yin X,Fernández-Caggiano M,Willeit P,Puntmann VO,Aldama-López G,Shah AM,Doménech N,Mayr M


book

A novel mutation of LAMB2 in a multigenerational mennonite family reveals a new phenotypic variant of Pierson syndrome.
Ophthalmology118(6)1137-44(2011 Jun)
Mohney BG,Pulido JS,Lindor NM,Hogan MC,Consugar MB,Peters J,Pankratz VS,Nasr SH,Smith SJ,Gloor J,Kubly V,Spencer D,Nielson R,Puffenberger EG,Strauss KA,Morton DH,Eldahdah L,Harris PC


 
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