×

LDHA cDNA ORF clone, Homo sapiens(Human)

Gene Symbol LDHA
Entrez Gene ID 3939
Full Name lactate dehydrogenase A
Synonyms GSD11,HEL-S-133P,LDHM,PIG19
General protein information
Preferred Names

lactate dehydrogenase A

Names

L-lactate dehydrogenase A chain
LDH muscle subunit
LDH-A
LDH-M
cell proliferation-inducing gene 19 protein
epididymis secretory sperm binding protein Li 133P
lactate dehydrogenase M
proliferation-inducing gene 19
renal carcinoma antigen NY-REN-59

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

11

11p15.1

Summary The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008].
Disorder MIM:

150000

Disorder Html: Glycogen storage disease XI, 612933 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001165416.1 NP_001158888.1 L-lactate dehydrogenase A chain isoform 5
NM_005566.3 NP_005557.1 L-lactate dehydrogenase A chain isoform 1
NM_001165414.1 NP_001158886.1 L-lactate dehydrogenase A chain isoform 3
NM_001135239.1 NP_001128711.1 L-lactate dehydrogenase A chain isoform 2
NM_001165415.1 NP_001158887.1 L-lactate dehydrogenase A chain isoform 4



Macaca mulatta (rhesus monkey) LDHA XP_001086967.1
Bos taurus (cattle) LDHA NP_776524.1
Mus musculus (house mouse) Ldha NP_001129541.2
Gallus gallus (chicken) LDHA NP_990615.1
Danio rerio (zebrafish) ldha NP_571321.1
Arabidopsis thaliana (thale cress) AT4G17260 NP_193459.1
Xenopus tropicalis (tropical clawed frog) ldha NP_989153.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004880 XP_001688556.1
Caenorhabditis elegans (roundworm) ldh-1 NP_496503.1
Pan troglodytes (chimpanzee) LDHA NP_001029268.1
Canis lupus familiaris (dog) LDHA XP_534084.4
Drosophila melanogaster (fruit fly) ImpL3 NP_476581.1
Oryza sativa (rice) Os02g0105400 NP_001045619.1
Homo sapiens (human) LDHA NP_001158886.1
Oryza sativa (rice) Os06g0104900 NP_001056552.1


Related articles in PubMed

Stable shRNA Silencing of Lactate Dehydrogenase A (LDHA) in Human MDA-MB-231 Breast Cancer Cells Fails to Alter Lactic Acid Production, Glycolytic Activity, ATP or Survival.
Mack N, Mazzio EA, Bauer D, Flores-Rozas H, Soliman KF
Anticancer research37(3)1205-1212(2017 03)

Lactate dehydrogenase inhibitors can reverse inflammation induced changes in colon cancer cells.
Manerba M, Di Ianni L, Govoni M, Roberti M, Recanatini M, Di Stefano G
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences9637-44(2017 Jan)

Relevance of serum biomarkers associated with melanoma during follow-up of anti-CTLA-4 immunotherapy.
Felix J, Cassinat B, Porcher R, Schlageter MH, Maubec E, Pages C, Baroudjian B, Homyrda L, Boukouaci W, Tamouza R, Bagot M, Caignard A, Toubert A, Lebbé C, Moins-Teisserenc H
International immunopharmacology40466-473(2016 Nov)

Overall survival of cancer patients with serum lactate dehydrogenase greater than 1000 IU/L.
Liu R, Cao J, Gao X, Zhang J, Wang L, Wang B, Guo L, Hu X, Wang Z
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(10)14083-14088(2016 Oct)

Prognostic Role of Lactate Dehydrogenase Expression in Urologic Cancers: A Systematic Review and Meta-Analysis.
Zhang Y, Xu T, Wang Y, Zhang H, Zhao Y, Yang X, Cao Y, Yang W, Niu H
Oncology research and treatment39(10)592-604(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-30a-5p may function as a tumor suppressor in breast cancer through the inhibition of LDHA expression and glycolysis.
Title: miR-30a-5p suppresses breast tumor growth and metastasis through inhibition of LDHA-mediated Warburg effect.

Data indicate that lactate dehydrogenase A (LDHA) is involved in the transcription of histone 2B gene.
Title: The inhibition of lactate dehydrogenase A hinders the transcription of histone 2B gene independently from the block of aerobic glycolysis.

alternative isoforms of LDH in cancer cells produce lactic acid, when LDHA is silenced or inhibited
Title: Stable shRNA Silencing of Lactate Dehydrogenase A (LDHA) in Human MDA-MB-231 Breast Cancer Cells Fails to Alter Lactic Acid Production, Glycolytic Activity, ATP or Survival.

this study shows that low levels of LDH correlate to a better anti-CTLA-4 immunotherapy response and survival in advanced melanoma patients
Title: Relevance of serum biomarkers associated with melanoma during follow-up of anti-CTLA-4 immunotherapy.

High serum LDH is associated with OS and PFS in patients with urinary system cancer, and it is an effective biomarker of prognosis in patients with urologic cancer.
Title: Prognostic Role of Lactate Dehydrogenase Expression in Urologic Cancers: A Systematic Review and Meta-Analysis.

The following LDHA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LDHA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18760 NM_005566.3
Latest version!
Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu15696 NM_001165416.1
Latest version!
Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu15968 NM_001135239.1
Latest version!
Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu15988 NM_001165415.1
Latest version!
Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $216.30
$309.00
OHu16013 NM_001165414.1
Latest version!
Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18760
Clone ID Related Accession (Same CDS sequence) NM_005566.3
Accession Version NM_005566.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 999bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-04
Organism Homo sapiens(Human)
Product L-lactate dehydrogenase A chain isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB502157.1, BC067223.1, BG424022.1, AC084117.6 and BM995301.1. This sequence is a reference standard in the RefSeqGene project. On Sep 27, 2008 this sequence version replaced NM_005566.2. Transcript Variant: This variant (1) encodes the predominant isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.168123.1, SRR1803616.91074.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGACTATA ATGTAACTGC AAACTCCAAG CTGGTCATTA TCACGGCTGG GGCACGTCAG
CAAGAGGGAG AAAGCCGTCT TAATTTGGTC CAGCGTAACG TGAACATCTT TAAATTCATC
ATTCCTAATG TTGTAAAATA CAGCCCGAAC TGCAAGTTGC TTATTGTTTC AAATCCAGTG
GATATCTTGA CCTACGTGGC TTGGAAGATA AGTGGTTTTC CCAAAAACCG TGTTATTGGA
AGCGGTTGCA ATCTGGATTC AGCCCGATTC CGTTACCTAA TGGGGGAAAG GCTGGGAGTT
CACCCATTAA GCTGTCATGG GTGGGTCCTT GGGGAACATG GAGATTCCAG TGTGCCTGTA
TGGAGTGGAA TGAATGTTGC TGGTGTCTCT CTGAAGACTC TGCACCCAGA TTTAGGGACT
GATAAAGATA AGGAACAGTG GAAAGAGGTT CACAAGCAGG TGGTTGAGAG TGCTTATGAG
GTGATCAAAC TCAAAGGCTA CACATCCTGG GCTATTGGAC TCTCTGTAGC AGATTTGGCA
GAGAGTATAA TGAAGAATCT TAGGCGGGTG CACCCAGTTT CCACCATGAT TAAGGGTCTT
TACGGAATAA AGGATGATGT CTTCCTTAGT GTTCCTTGCA TTTTGGGACA GAATGGAATC
TCAGACCTTG TGAAGGTGAC TCTGACTTCT GAGGAAGAGG CCCGTTTGAA GAAGAGTGCA
GATACACTTT GGGGGATCCA AAAGGAGCTG CAATTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005557.1
CDS273..1271
Misc Feature(1)135..137
Misc Feature(2)276..278
Misc Feature(3)285..287
Misc Feature(4)285..287
Misc Feature(5)300..302
Misc Feature(6)312..314
Misc Feature(7)324..326
Misc Feature(8)327..1259
Misc Feature(9)order(330..332,813..815,822..824,828..830,879..881,894..896,1065..1073,1080..1082,1161..1166,1173..1175,1179..1187)
Misc Feature(10)345..1244
Misc Feature(11)order(357..365,426..428,432..434,441..443,555..569,678..680,684..686,753..755,765..767,849..851,1014..1016,1026..1028)
Misc Feature(12)order(369..371,384..386,450..455,462..464,468..473,777..779,783..788,804..806,1002..1007,1014..1025)
Misc Feature(13)441..443
Misc Feature(14)513..515
Misc Feature(15)order(570..572,588..590,684..686,777..779,849..851,984..986,1014..1016)
Misc Feature(16)624..626
Misc Feature(17)624..626
Misc Feature(18)648..650
Misc Feature(19)942..944
Misc Feature(20)966..968
Misc Feature(21)987..989
Misc Feature(22)987..989
Misc Feature(23)987..989
Misc Feature(24)999..1001
Misc Feature(25)1197..1199
Misc Feature(26)1200..1202
Misc Feature(27)1224..1226
Misc Feature(28)1224..1226
Misc Feature(29)1236..1238
Exon (1)1..248
Exon (2)249..398
Exon (3)399..516
Exon (4)517..690
Exon (5)691..864
Exon (6)865..982
Exon (7)983..1106
Exon (8)1107..2208
Translation

Target ORF information:

RefSeq Version NM_005566.3
Organism Homo sapiens(Human)
Definition Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005566.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGACTATA ATGTAACTGC AAACTCCAAG CTGGTCATTA TCACGGCTGG GGCACGTCAG
CAAGAGGGAG AAAGCCGTCT TAATTTGGTC CAGCGTAACG TGAACATCTT TAAATTCATC
ATTCCTAATG TTGTAAAATA CAGCCCGAAC TGCAAGTTGC TTATTGTTTC AAATCCAGTG
GATATCTTGA CCTACGTGGC TTGGAAGATA AGTGGTTTTC CCAAAAACCG TGTTATTGGA
AGCGGTTGCA ATCTGGATTC AGCCCGATTC CGTTACCTAA TGGGGGAAAG GCTGGGAGTT
CACCCATTAA GCTGTCATGG GTGGGTCCTT GGGGAACATG GAGATTCCAG TGTGCCTGTA
TGGAGTGGAA TGAATGTTGC TGGTGTCTCT CTGAAGACTC TGCACCCAGA TTTAGGGACT
GATAAAGATA AGGAACAGTG GAAAGAGGTT CACAAGCAGG TGGTTGAGAG TGCTTATGAG
GTGATCAAAC TCAAAGGCTA CACATCCTGG GCTATTGGAC TCTCTGTAGC AGATTTGGCA
GAGAGTATAA TGAAGAATCT TAGGCGGGTG CACCCAGTTT CCACCATGAT TAAGGGTCTT
TACGGAATAA AGGATGATGT CTTCCTTAGT GTTCCTTGCA TTTTGGGACA GAATGGAATC
TCAGACCTTG TGAAGGTGAC TCTGACTTCT GAGGAAGAGG CCCGTTTGAA GAAGAGTGCA
GATACACTTT GGGGGATCCA AAAGGAGCTG CAATTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15696
Clone ID Related Accession (Same CDS sequence) NM_001165416.1
Accession Version NM_001165416.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 726bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product L-lactate dehydrogenase A chain isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB502157.1, BG760757.1, AC084117.6 and BM995301.1. Transcript Variant: This variant (5) lacks an alternate exon in the 3' coding region, compared to variant 1, which results in a frameshift. The resulting isoform (5) lacks a segment of the LDH domain and has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL559956.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGACTATA ATGTAACTGC AAACTCCAAG CTGGTCATTA TCACGGCTGG GGCACGTCAG
CAAGAGGGAG AAAGCCGTCT TAATTTGGTC CAGCGTAACG TGAACATCTT TAAATTCATC
ATTCCTAATG TTGTAAAATA CAGCCCGAAC TGCAAGTTGC TTATTGTTTC AAATCCAGTG
GATATCTTGA CCTACGTGGC TTGGAAGATA AGTGGTTTTC CCAAAAACCG TGTTATTGGA
AGCGGTTGCA ATCTGGATTC AGCCCGATTC CGTTACCTAA TGGGGGAAAG GCTGGGAGTT
CACCCATTAA GCTGTCATGG GTGGGTCCTT GGGGAACATG GAGATTCCAG TGTGCCTGTA
TGGAGTGGAA TGAATGTTGC TGGTGTCTCT CTGAAGACTC TGCACCCAGA TTTAGGGACT
GATAAAGATA AGGAACAGTG GAAAGAGGTT CACAAGCAGG TGGTTGAGAG GGTCTTTACG
GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001158888.1
CDS273..998
Misc Feature(1)135..137
Misc Feature(2)276..278
Misc Feature(3)285..287
Misc Feature(4)285..287
Misc Feature(5)300..302
Misc Feature(6)312..314
Misc Feature(7)324..326
Misc Feature(8)327..>992
Misc Feature(9)order(357..365,426..428,432..434,441..443,555..569,678..680,684..686,753..755,765..767,849..851)
Misc Feature(10)441..443
Misc Feature(11)513..515
Misc Feature(12)order(570..572,588..590,684..686,777..779,849..851,984..986)
Misc Feature(13)624..626
Misc Feature(14)624..626
Misc Feature(15)648..650
Misc Feature(16)942..944
Misc Feature(17)966..968
Misc Feature(18)987..989
Exon (1)1..248
Exon (2)249..398
Exon (3)399..516
Exon (4)517..690
Exon (5)691..864
Exon (6)865..982
Exon (7)983..2084
Translation

Target ORF information:

RefSeq Version NM_001165416.1
Organism Homo sapiens(Human)
Definition Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001165416.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGACTATA ATGTAACTGC AAACTCCAAG CTGGTCATTA TCACGGCTGG GGCACGTCAG
CAAGAGGGAG AAAGCCGTCT TAATTTGGTC CAGCGTAACG TGAACATCTT TAAATTCATC
ATTCCTAATG TTGTAAAATA CAGCCCGAAC TGCAAGTTGC TTATTGTTTC AAATCCAGTG
GATATCTTGA CCTACGTGGC TTGGAAGATA AGTGGTTTTC CCAAAAACCG TGTTATTGGA
AGCGGTTGCA ATCTGGATTC AGCCCGATTC CGTTACCTAA TGGGGGAAAG GCTGGGAGTT
CACCCATTAA GCTGTCATGG GTGGGTCCTT GGGGAACATG GAGATTCCAG TGTGCCTGTA
TGGAGTGGAA TGAATGTTGC TGGTGTCTCT CTGAAGACTC TGCACCCAGA TTTAGGGACT
GATAAAGATA AGGAACAGTG GAAAGAGGTT CACAAGCAGG TGGTTGAGAG GGTCTTTACG
GAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15968
Clone ID Related Accession (Same CDS sequence) NM_001135239.1
Accession Version NM_001135239.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 825bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-04
Organism Homo sapiens(Human)
Product L-lactate dehydrogenase A chain isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB502157.1, AK296667.1, AC084117.6 and BM995301.1. Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The resulting isoform (2) lacks a segment of the LDH domain but has the same N- and C-termini, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296667.1, SRR1163658.295738.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGTGGATA TCTTGACCTA CGTGGCTTGG AAGATAAGTG GTTTTCCCAA AAACCGTGTT
ATTGGAAGCG GTTGCAATCT GGATTCAGCC CGATTCCGTT ACCTAATGGG GGAAAGGCTG
GGAGTTCACC CATTAAGCTG TCATGGGTGG GTCCTTGGGG AACATGGAGA TTCCAGTGTG
CCTGTATGGA GTGGAATGAA TGTTGCTGGT GTCTCTCTGA AGACTCTGCA CCCAGATTTA
GGGACTGATA AAGATAAGGA ACAGTGGAAA GAGGTTCACA AGCAGGTGGT TGAGAGTGCT
TATGAGGTGA TCAAACTCAA AGGCTACACA TCCTGGGCTA TTGGACTCTC TGTAGCAGAT
TTGGCAGAGA GTATAATGAA GAATCTTAGG CGGGTGCACC CAGTTTCCAC CATGATTAAG
GGTCTTTACG GAATAAAGGA TGATGTCTTC CTTAGTGTTC CTTGCATTTT GGGACAGAAT
GGAATCTCAG ACCTTGTGAA GGTGACTCTG ACTTCTGAGG AAGAGGCCCG TTTGAAGAAG
AGTGCAGATA CACTTTGGGG GATCCAAAAG GAGCTGCAAT TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001128711.1
CDS273..1097
Misc Feature(1)135..137
Misc Feature(2)327..1085
Misc Feature(3)order(330..332,639..641,648..650,654..656,705..707,720..722,891..899,906..908,987..992,999..1001,1005..1013)
Misc Feature(4)345..1070
Misc Feature(5)order(369..371,384..386,450..455,462..464,468..473,603..605,609..614,630..632,828..833,840..851)
Exon (1)1..248
Exon (2)249..398
Exon (3)399..516
Exon (4)517..690
Exon (5)691..808
Exon (6)809..932
Exon (7)933..2034
Translation

Target ORF information:

RefSeq Version NM_001135239.1
Organism Homo sapiens(Human)
Definition Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001135239.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGTGGATA TCTTGACCTA CGTGGCTTGG AAGATAAGTG GTTTTCCCAA AAACCGTGTT
ATTGGAAGCG GTTGCAATCT GGATTCAGCC CGATTCCGTT ACCTAATGGG GGAAAGGCTG
GGAGTTCACC CATTAAGCTG TCATGGGTGG GTCCTTGGGG AACATGGAGA TTCCAGTGTG
CCTGTATGGA GTGGAATGAA TGTTGCTGGT GTCTCTCTGA AGACTCTGCA CCCAGATTTA
GGGACTGATA AAGATAAGGA ACAGTGGAAA GAGGTTCACA AGCAGGTGGT TGAGAGTGCT
TATGAGGTGA TCAAACTCAA AGGCTACACA TCCTGGGCTA TTGGACTCTC TGTAGCAGAT
TTGGCAGAGA GTATAATGAA GAATCTTAGG CGGGTGCACC CAGTTTCCAC CATGATTAAG
GGTCTTTACG GAATAAAGGA TGATGTCTTC CTTAGTGTTC CTTGCATTTT GGGACAGAAT
GGAATCTCAG ACCTTGTGAA GGTGACTCTG ACTTCTGAGG AAGAGGCCCG TTTGAAGAAG
AGTGCAGATA CACTTTGGGG GATCCAAAAG GAGCTGCAAT TTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu15988
Clone ID Related Accession (Same CDS sequence) NM_001165415.1
Accession Version NM_001165415.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 825bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product L-lactate dehydrogenase A chain isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB502157.1, AK130587.1, AK307477.1, AC084117.6 and BM995301.1. Transcript Variant: This variant (4) differs in the 3' UTR and has multiple differences in the 3' coding region, compared to variant 1, one of which results in a frameshift. The resulting isoform (4) lacks a segment of the LDH domain and has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK130587.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGACTATA ATGTAACTGC AAACTCCAAG CTGGTCATTA TCACGGCTGG GGCACGTCAG
CAAGAGGGAG AAAGCCGTCT TAATTTGGTC CAGCGTAACG TGAACATCTT TAAATTCATC
ATTCCTAATG TTGTAAAATA CAGCCCGAAC TGCAAGTTGC TTATTGTTTC AAATCCAGTG
GATATCTTGA CCTACGTGGC TTGGAAGATA AGTGGTTTTC CCAAAAACCG TGTTATTGGA
AGCGGTTGCA ATCTGGATTC AGCCCGATTC CGTTACCTAA TGGGGGAAAG GCTGGGAGTT
CACCCATTAA GCTGTCATGG GTGGGTCCTT GGGGAACATG GAGATTCCAG TGTGCCTGTA
TGGAGTGGAA TGAATGTTGC TGGTGTCTCT CTGAAGACTC TGCACCCAGA TTTAGGGACT
GATAAAGATA AGGAACAGTG GAAAGAGTGC AGATACACTT TGGGGGATCC AAAAGGAGCT
GCAATTTTAA AGTCTTCTGA TGTCATATCA TTTCACTGTC TAGGCTACAA CAGGATTCTA
GGTGGAGGTT GTGCATGTTG TCCTTTTTAT CTGATCTGTG ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001158887.1
CDS273..1097
Misc Feature(1)135..137
Misc Feature(2)276..278
Misc Feature(3)285..287
Misc Feature(4)285..287
Misc Feature(5)300..302
Misc Feature(6)312..314
Misc Feature(7)324..326
Misc Feature(8)327..>959
Misc Feature(9)order(357..365,426..428,432..434,441..443,555..569,678..680,684..686,753..755,765..767,849..851)
Misc Feature(10)441..443
Misc Feature(11)513..515
Misc Feature(12)624..626
Misc Feature(13)624..626
Misc Feature(14)648..650
Misc Feature(15)942..944
Exon (1)1..248
Exon (2)249..398
Exon (3)399..516
Exon (4)517..690
Exon (5)691..864
Exon (6)865..959
Exon (7)960..1939
Translation

Target ORF information:

RefSeq Version NM_001165415.1
Organism Homo sapiens(Human)
Definition Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001165415.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGGCAACTC TAAAGGATCA GCTGATTTAT AATCTTCTAA AGGAAGAACA GACCCCCCAG 
AATAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CCTGTGCCAT CAGTATCTTA
ATGAAGGACT TGGCAGATGA ACTTGCTCTT GTTGATGTCA TCGAAGACAA ATTGAAGGGA
GAGATGATGG ATCTCCAACA TGGCAGCCTT TTCCTTAGAA CACCAAAGAT TGTCTCTGGC
AAAGACTATA ATGTAACTGC AAACTCCAAG CTGGTCATTA TCACGGCTGG GGCACGTCAG
CAAGAGGGAG AAAGCCGTCT TAATTTGGTC CAGCGTAACG TGAACATCTT TAAATTCATC
ATTCCTAATG TTGTAAAATA CAGCCCGAAC TGCAAGTTGC TTATTGTTTC AAATCCAGTG
GATATCTTGA CCTACGTGGC TTGGAAGATA AGTGGTTTTC CCAAAAACCG TGTTATTGGA
AGCGGTTGCA ATCTGGATTC AGCCCGATTC CGTTACCTAA TGGGGGAAAG GCTGGGAGTT
CACCCATTAA GCTGTCATGG GTGGGTCCTT GGGGAACATG GAGATTCCAG TGTGCCTGTA
TGGAGTGGAA TGAATGTTGC TGGTGTCTCT CTGAAGACTC TGCACCCAGA TTTAGGGACT
GATAAAGATA AGGAACAGTG GAAAGAGTGC AGATACACTT TGGGGGATCC AAAAGGAGCT
GCAATTTTAA AGTCTTCTGA TGTCATATCA TTTCACTGTC TAGGCTACAA CAGGATTCTA
GGTGGAGGTT GTGCATGTTG TCCTTTTTAT CTGATCTGTG ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu16013
Clone ID Related Accession (Same CDS sequence) NM_001165414.1
Accession Version NM_001165414.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1086bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-23
Organism Homo sapiens(Human)
Product L-lactate dehydrogenase A chain isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC392310.1, DC396757.1, AK298834.1, AK307477.1, AC084117.6 and BM995301.1. Transcript Variant: This variant (3) uses an alternate 5' exon that results in a distinct 5' UTR and causes translation initiation at an alternate start codon, compared to variant 1. The resulting isoform (3) has a longer N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298834.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGTGAAC CCTCAGGAGG CTATACTTAC ACCCAAACGT CGATATTCCT TTTCCACGCT 
AAGATTCCTT TTGGTTCCAA GTCCAATATG GCAACTCTAA AGGATCAGCT GATTTATAAT
CTTCTAAAGG AAGAACAGAC CCCCCAGAAT AAGATTACAG TTGTTGGGGT TGGTGCTGTT
GGCATGGCCT GTGCCATCAG TATCTTAATG AAGGACTTGG CAGATGAACT TGCTCTTGTT
GATGTCATCG AAGACAAATT GAAGGGAGAG ATGATGGATC TCCAACATGG CAGCCTTTTC
CTTAGAACAC CAAAGATTGT CTCTGGCAAA GACTATAATG TAACTGCAAA CTCCAAGCTG
GTCATTATCA CGGCTGGGGC ACGTCAGCAA GAGGGAGAAA GCCGTCTTAA TTTGGTCCAG
CGTAACGTGA ACATCTTTAA ATTCATCATT CCTAATGTTG TAAAATACAG CCCGAACTGC
AAGTTGCTTA TTGTTTCAAA TCCAGTGGAT ATCTTGACCT ACGTGGCTTG GAAGATAAGT
GGTTTTCCCA AAAACCGTGT TATTGGAAGC GGTTGCAATC TGGATTCAGC CCGATTCCGT
TACCTAATGG GGGAAAGGCT GGGAGTTCAC CCATTAAGCT GTCATGGGTG GGTCCTTGGG
GAACATGGAG ATTCCAGTGT GCCTGTATGG AGTGGAATGA ATGTTGCTGG TGTCTCTCTG
AAGACTCTGC ACCCAGATTT AGGGACTGAT AAAGATAAGG AACAGTGGAA AGAGGTTCAC
AAGCAGGTGG TTGAGAGTGC TTATGAGGTG ATCAAACTCA AAGGCTACAC ATCCTGGGCT
ATTGGACTCT CTGTAGCAGA TTTGGCAGAG AGTATAATGA AGAATCTTAG GCGGGTGCAC
CCAGTTTCCA CCATGATTAA GGGTCTTTAC GGAATAAAGG ATGATGTCTT CCTTAGTGTT
CCTTGCATTT TGGGACAGAA TGGAATCTCA GACCTTGTGA AGGTGACTCT GACTTCTGAG
GAAGAGGCCC GTTTGAAGAA GAGTGCAGAT ACACTTTGGG GGATCCAAAA GGAGCTGCAA
TTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001158886.1
CDS283..1368
Misc Feature(1)274..276
Misc Feature(2)424..1356
Misc Feature(3)order(427..429,910..912,919..921,925..927,976..978,991..993,1162..1170,1177..1179,1258..1263,1270..1272,1276..1284)
Misc Feature(4)442..1341
Misc Feature(5)order(454..462,523..525,529..531,538..540,652..666,775..777,781..783,850..852,862..864,946..948,1111..1113,1123..1125)
Misc Feature(6)order(466..468,481..483,547..552,559..561,565..570,874..876,880..885,901..903,1099..1104,1111..1122)
Misc Feature(7)order(667..669,685..687,781..783,874..876,946..948,1081..1083,1111..1113)
Exon (1)1..345
Exon (2)346..495
Exon (3)496..613
Exon (4)614..787
Exon (5)788..961
Exon (6)962..1079
Exon (7)1080..1203
Exon (8)1204..2305
Translation

Target ORF information:

RefSeq Version NM_001165414.1
Organism Homo sapiens(Human)
Definition Homo sapiens lactate dehydrogenase A (LDHA), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001165414.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
ATGGGTGAAC CCTCAGGAGG CTATACTTAC ACCCAAACGT CGATATTCCT TTTCCACGCT 
AAGATTCCTT TTGGTTCCAA GTCCAATATG GCAACTCTAA AGGATCAGCT GATTTATAAT
CTTCTAAAGG AAGAACAGAC CCCCCAGAAT AAGATTACAG TTGTTGGGGT TGGTGCTGTT
GGCATGGCCT GTGCCATCAG TATCTTAATG AAGGACTTGG CAGATGAACT TGCTCTTGTT
GATGTCATCG AAGACAAATT GAAGGGAGAG ATGATGGATC TCCAACATGG CAGCCTTTTC
CTTAGAACAC CAAAGATTGT CTCTGGCAAA GACTATAATG TAACTGCAAA CTCCAAGCTG
GTCATTATCA CGGCTGGGGC ACGTCAGCAA GAGGGAGAAA GCCGTCTTAA TTTGGTCCAG
CGTAACGTGA ACATCTTTAA ATTCATCATT CCTAATGTTG TAAAATACAG CCCGAACTGC
AAGTTGCTTA TTGTTTCAAA TCCAGTGGAT ATCTTGACCT ACGTGGCTTG GAAGATAAGT
GGTTTTCCCA AAAACCGTGT TATTGGAAGC GGTTGCAATC TGGATTCAGC CCGATTCCGT
TACCTAATGG GGGAAAGGCT GGGAGTTCAC CCATTAAGCT GTCATGGGTG GGTCCTTGGG
GAACATGGAG ATTCCAGTGT GCCTGTATGG AGTGGAATGA ATGTTGCTGG TGTCTCTCTG
AAGACTCTGC ACCCAGATTT AGGGACTGAT AAAGATAAGG AACAGTGGAA AGAGGTTCAC
AAGCAGGTGG TTGAGAGTGC TTATGAGGTG ATCAAACTCA AAGGCTACAC ATCCTGGGCT
ATTGGACTCT CTGTAGCAGA TTTGGCAGAG AGTATAATGA AGAATCTTAG GCGGGTGCAC
CCAGTTTCCA CCATGATTAA GGGTCTTTAC GGAATAAAGG ATGATGTCTT CCTTAGTGTT
CCTTGCATTT TGGGACAGAA TGGAATCTCA GACCTTGTGA AGGTGACTCT GACTTCTGAG
GAAGAGGCCC GTTTGAAGAA GAGTGCAGAT ACACTTTGGG GGATCCAAAA GGAGCTGCAA
TTTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Stable shRNA Silencing of Lactate Dehydrogenase A (LDHA) in Human MDA-MB-231 Breast Cancer Cells Fails to Alter Lactic Acid Production, Glycolytic Activity, ATP or Survival.
Anticancer research37(3)1205-1212(2017 03)
Mack N,Mazzio EA,Bauer D,Flores-Rozas H,Soliman KF


book

Lactate dehydrogenase inhibitors can reverse inflammation induced changes in colon cancer cells.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences9637-44(2017 Jan)
Manerba M,Di Ianni L,Govoni M,Roberti M,Recanatini M,Di Stefano G


book

Relevance of serum biomarkers associated with melanoma during follow-up of anti-CTLA-4 immunotherapy.
International immunopharmacology40466-473(2016 Nov)
Felix J,Cassinat B,Porcher R,Schlageter MH,Maubec E,Pages C,Baroudjian B,Homyrda L,Boukouaci W,Tamouza R,Bagot M,Caignard A,Toubert A,Lebbé C,Moins-Teisserenc H


book

Overall survival of cancer patients with serum lactate dehydrogenase greater than 1000 IU/L.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(10)14083-14088(2016 Oct)
Liu R,Cao J,Gao X,Zhang J,Wang L,Wang B,Guo L,Hu X,Wang Z


book

Prognostic Role of Lactate Dehydrogenase Expression in Urologic Cancers: A Systematic Review and Meta-Analysis.
Oncology research and treatment39(10)592-604(2016)
Zhang Y,Xu T,Wang Y,Zhang H,Zhao Y,Yang X,Cao Y,Yang W,Niu H


 
*
*
*
*