SHC4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Gene Symbol	SHC4
Entrez Gene ID	399694
Full Name	SHC adaptor protein 4
Synonyms	RaLP,SHCD
General protein information	Preferred Names SHC adaptor protein 4 Names SHC-transforming protein 4 SH2 domain protein C4 SHC (Src homology 2 domain containing) family, member 4 SHC-transforming protein D hShcD rai-like protein src homology 2 domain-containing-transforming protein C4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q21.1
Disorder MIM:	617372

mRNA and Protein(s)

mRNA	Protein	Name
XM_005254375.3	XP_005254432.1	SHC-transforming protein 4 isoform X1
NM_203349.3	NP_976224.3	SHC-transforming protein 4
NM_203349.4	NP_976224.3	SHC-transforming protein 4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SHC4	XP_544671.2
Rattus norvegicus (Norway rat)	Shc4	NP_001177994.1
Homo sapiens (human)	SHC4	NP_976224.3
Mus musculus (house mouse)	Shc4	NP_950187.1
Gallus gallus (chicken)	SHC4	XP_004943863.1
Drosophila melanogaster (fruit fly)	Shc	NP_524683.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011463	XP_317844.3
Pan troglodytes (chimpanzee)	SHC4	XP_510385.2
	SHC4	XP_001113330.1
Bos taurus (cattle)	SHC4	NP_001192841.1
Xenopus tropicalis (tropical clawed frog)	shc4	XP_002936616.2

Gene Ontology
Bibliography

Related articles in PubMed

The ShcD phosphotyrosine adaptor subverts canonical EGF receptor trafficking.
Wills MKB, Lau HR, Jones N
Journal of cell science130(17)2808-2820(2017 Sep)

Signaling adaptor ShcD suppresses extracellular signal-regulated kinase (Erk) phosphorylation distal to the Ret and Trk neurotrophic receptors.
Wills MK, Keyvani Chahi A, Lau HR, Tilak M, Guild BD, New LA, Lu P, Jacquet K, Meakin SO, Bisson N, Jones N
The Journal of biological chemistry292(14)5748-5759(2017 04)

Enhanced prediction of Src homology 2 (SH2) domain binding potentials using a fluorescence polarization-derived c-Met, c-Kit, ErbB, and androgen receptor interactome.
Leung KK, Hause RJ Jr, Barkinge JL, Ciaccio MF, Chuu CP, Jones RB
Molecular & cellular proteomics : MCP13(7)1705-23(2014 Jul)

The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells.
Petschnigg J, Groisman B, Kotlyar M, Taipale M, Zheng Y, Kurat CF, Sayad A, Sierra JR, Mattiazzi Usaj M, Snider J, Nachman A, Krykbaeva I, Tsao MS, Moffat J, Pawson T, Lindquist S, Jurisica I, Stagljar I
Nature methods11(5)585-92(2014 May)

The ShcD signaling adaptor facilitates ligand-independent phosphorylation of the EGF receptor.
Wills MK, Tong J, Tremblay SL, Moran MF, Jones N
Molecular biology of the cell25(6)739-52(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ShcD binds to active Ret, TrkA, and TrkB neurotrophic factor receptors predominantly via its phosphotyrosine-binding (PTB) domain.
Title: Signaling adaptor ShcD suppresses extracellular signal-regulated kinase (Erk) phosphorylation distal to the Ret and Trk neurotrophic receptors.

The ShcD signaling adaptor facilitates ligand-independent phosphorylation of the EGF receptor.
Title: The ShcD signaling adaptor facilitates ligand-independent phosphorylation of the EGF receptor.

ShcD can exist within the nucleus, and its CH2 domain has a critical role in nuclear export of ShcD.
Title: A nuclear export signal and oxidative stress regulate ShcD subcellular localisation: a potential role for ShcD in the nucleus.

ShcD interacts with TrkB in a kinase-activity-dependent manner.
Title: ShcD interacts with TrkB via its PTB and SH2 domains and regulates BDNF-induced MAPK activation.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following SHC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SHC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07749	NM_203349.4 Latest version!	Homo sapiens SHC adaptor protein 4 (SHC4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu48289	XM_005254375.3 Latest version!	Homo sapiens SHC adaptor protein 4 (SHC4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu07749	NM_203349.3	Homo sapiens SHC adaptor protein 4 (SHC4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SHC4 ( NM_203349.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_976224.3 Homo sapiens SHC adaptor protein 4 (SHC4), mRNA.

Price & Availability↑

CloneID	OHu07749
Clone ID Related Accession (Same CDS sequence)	NM_203349.3 , NM_203349.4
Accession Version	NM_203349.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	SHC-transforming protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC091073.3, AY464565.1 and AC012379.7. On Feb 4, 2009 this sequence version replaced NM_203349.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033907.2, SRR1803614.216458.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGAGAAC GCGGCCAGGA CAGCCTGGCA GGACTCGTGC TGTATGTAGG ACTCTTCGGG 
CACCCCGGGA TGCTGCACAG GGCCAAGTAC AGCCGCTTTC GGAACGAGTC GATCACGTCC 
TTGGACGAAG GTAGCTCCGG AGGCTCGGTC GGGAACAAGG GCTCGCCGCA GCCTCCCCAC 
CCCGCCCTGG CACCTCACCT GCCGACTGAA GATGCCACCT TGCCGTCGCA GGAGAGCCCC 
ACCCCACTGT GCACCTTGAT CCCCCGCATG GCAAGCATGA AGCTGGCCAA CCCGGCCACT 
TTGCTGAGTC TGAAAAACTT TTGCCTGGGT ACCAAAGAGG TGCCTCGGCT GAAGCTCCAG 
GAAAGCCGGG ACCCAGGTTC CAGCGGCCCC TCTTCCCCAG AAACCAGTTT AAGTAGGTCC 
GGGACTGCAC CTCCACCGCA GCAGGACCTG GTGGGACACA GGGCAACCGC CCTAACCCCT 
GATTCGTGCC CGCTTCCTGG CCCTGGGGAG CCAACACTTA GGAGCAGGCA GGACAGGCAC 
TTTCTACAGC ACCTGTTGGG GATGGGCATG AACTACTGTG TGAGGTACAT GGGCTGTGTT 
GAAGTGCTGC AATCAATGAG ATCACTGGAT TTTGGAATGA GAACCCAAGT TACAAGGGAA 
GCAATAAGTC GCCTGTGTGA AGCTGTCCCC GGGGCAAATG GAGCCATTAA AAAGCGAAAG 
CCTCCAGTTA AGTTCCTATC AACAGTCCTT GGCAAAAGTA ATCTTCAGTT TTCAGGAATG 
AATATAAAAC TGACCATCTC AACATGCAGT CTCACATTGA TGAATCTTGA CAACCAACAG 
ATTATTGCAA ATCATCATAT GCAGTCTATT TCATTTGCCT CTGGAGGGGA TCCTGATACT 
ACAGACTATG TTGCCTACGT AGCTAAAGAT CCAGTTAATC AACGAGCCTG TCACATATTG 
GAATGCCACA ATGGAATGGC CCAAGACGTC ATAAGTACCA TAGGGCAGGC TTTTGAACTC 
CGGTTTAAAC AGTACTTGAA AAATCCTTCT TTGAATACTT CTTGTGAAAG TGAGGAGGTG 
CATATTGATA GCCATGCCGA GGAGAGAGAA GATCATGAAT ATTACAATGA AATTCCAGGG 
AAGCAGCCAC CAGTAGGTGG TGTTTCAGAT ATGCGGATCA AAGTTCAAGC CACGGAACAA 
ATGGCTTACT GCCCCATACA GTGTGAAAAG TTGTGCTATT TGCCTGGAAA CTCCAAGTGC 
AGCAGTGTAT ATGAGAACTG TTTAGAACAA AGCAGGGCAA TAGGTAATGT CCATCCAAGA 
GGGGTGCAGT CCCAGCGAGA TACCTCATTA TTGAAGCACA CGTGCCGAGT GGATCTCTTT 
GATGACCCCT GCTACATTAA TACACAGGCT CTTCAAAGTA CACCTGGCTC TGCTGGAAAT 
CAAAGGTCAG CCCAACCACT GGGGAGCCCA TGGCACTGCG GAAAGGCACC AGAAACTGTT 
CAGCCGGGTG CCACAGCCCA GCCTGCCAGC TCACATTCTT TGCCACACAT TAAGCAGCAG 
CTGTGGAGCG AAGAATGCTA TCATGGCAAG CTGAGCAGGA AGGCGGCAGA GAGCCTCTTG 
GTAAAGGATG GGGACTTTTT GGTTCGAGAG AGTGCAACAT CCCCTGGCCA ATATGTGCTG 
AGTGGACTAC AGGGAGGCCA AGCAAAACAT CTTCTCCTGG TGGATCCTGA AGGCAAGGTG 
AGGACCAAGG ATCATGTATT TGATAATGTC GGCCACCTTA TCAGATACCA TATGGATAAC 
AGTTTGCCAA TCATCTCCTC TGGAAGCGAA GTAAGCCTTA AACAACCAGT GAGAAAAGAT 
AATAATCCAG CACTTTTGCA TTCCAACAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_976224.3
CDS	430..2322
Translation	MRERGQDSLAGLVLYVGLFGHPGMLHRAKYSRFRNESITSLDEGSSGGSVGNKGSPQPPHPALAPHLPTEDATLPSQESPTPLCTLIPRMASMKLANPATLLSLKNFCLGTKEVPRLKLQESRDPGSSGPSSPETSLSRSGTAPPPQQDLVGHRATALTPDSCPLPGPGEPTLRSRQDRHFLQHLLGMGMNYCVRYMGCVEVLQSMRSLDFGMRTQVTREAISRLCEAVPGANGAIKKRKPPVKFLSTVLGKSNLQFSGMNIKLTISTCSLTLMNLDNQQIIANHHMQSISFASGGDPDTTDYVAYVAKDPVNQRACHILECHNGMAQDVISTIGQAFELRFKQYLKNPSLNTSCESEEVHIDSHAEEREDHEYYNEIPGKQPPVGGVSDMRIKVQATEQMAYCPIQCEKLCYLPGNSKCSSVYENCLEQSRAIGNVHPRGVQSQRDTSLLKHTCRVDLFDDPCYINTQALQSTPGSAGNQRSAQPLGSPWHCGKAPETVQPGATAQPASSHSLPHIKQQLWSEECYHGKLSRKAAESLLVKDGDFLVRESATSPGQYVLSGLQGGQAKHLLLVDPEGKVRTKDHVFDNVGHLIRYHMDNSLPIISSGSEVSLKQPVRKDNNPALLHSNK

Target ORF information:

RefSeq Version	NM_203349.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SHC adaptor protein 4 (SHC4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203349.3

ORF Insert Sequence:

ATGCGAGAAC GCGGCCAGGA CAGCCTGGCA GGACTCGTGC TGTATGTAGG ACTCTTCGGG 
CACCCCGGGA TGCTGCACAG GGCCAAGTAC AGCCGCTTTC GGAACGAGTC GATCACGTCC 
TTGGACGAAG GTAGCTCCGG AGGCTCGGTC GGGAACAAGG GCTCGCCGCA GCCTCCCCAC 
CCCGCCCTGG CACCTCACCT GCCGACTGAA GATGCCACCT TGCCGTCGCA GGAGAGCCCC 
ACCCCACTGT GCACCTTGAT CCCCCGCATG GCAAGCATGA AGCTGGCCAA CCCGGCCACT 
TTGCTGAGTC TGAAAAACTT TTGCCTGGGT ACCAAAGAGG TGCCTCGGCT GAAGCTCCAG 
GAAAGCCGGG ACCCAGGTTC CAGCGGCCCC TCTTCCCCAG AAACCAGTTT AAGTAGGTCC 
GGGACTGCAC CTCCACCGCA GCAGGACCTG GTGGGACACA GGGCAACCGC CCTAACCCCT 
GATTCGTGCC CGCTTCCTGG CCCTGGGGAG CCAACACTTA GGAGCAGGCA GGACAGGCAC 
TTTCTACAGC ACCTGTTGGG GATGGGCATG AACTACTGTG TGAGGTACAT GGGCTGTGTT 
GAAGTGCTGC AATCAATGAG ATCACTGGAT TTTGGAATGA GAACCCAAGT TACAAGGGAA 
GCAATAAGTC GCCTGTGTGA AGCTGTCCCC GGGGCAAATG GAGCCATTAA AAAGCGAAAG 
CCTCCAGTTA AGTTCCTATC AACAGTCCTT GGCAAAAGTA ATCTTCAGTT TTCAGGAATG 
AATATAAAAC TGACCATCTC AACATGCAGT CTCACATTGA TGAATCTTGA CAACCAACAG 
ATTATTGCAA ATCATCATAT GCAGTCTATT TCATTTGCCT CTGGAGGGGA TCCTGATACT 
ACAGACTATG TTGCCTACGT AGCTAAAGAT CCAGTTAATC AACGAGCCTG TCACATATTG 
GAATGCCACA ATGGAATGGC CCAAGACGTC ATAAGTACCA TAGGGCAGGC TTTTGAACTC 
CGGTTTAAAC AGTACTTGAA AAATCCTTCT TTGAATACTT CTTGTGAAAG TGAGGAGGTG 
CATATTGATA GCCATGCCGA GGAGAGAGAA GATCATGAAT ATTACAATGA AATTCCAGGG 
AAGCAGCCAC CAGTAGGTGG TGTTTCAGAT ATGCGGATCA AAGTTCAAGC CACGGAACAA 
ATGGCTTACT GCCCCATACA GTGTGAAAAG TTGTGCTATT TGCCTGGAAA CTCCAAGTGC 
AGCAGTGTAT ATGAGAACTG TTTAGAACAA AGCAGGGCAA TAGGTAATGT CCATCCAAGA 
GGGGTGCAGT CCCAGCGAGA TACCTCATTA TTGAAGCACA CGTGCCGAGT GGATCTCTTT 
GATGACCCCT GCTACATTAA TACACAGGCT CTTCAAAGTA CACCTGGCTC TGCTGGAAAT 
CAAAGGTCAG CCCAACCACT GGGGAGCCCA TGGCACTGCG GAAAGGCACC AGAAACTGTT 
CAGCCGGGTG CCACAGCCCA GCCTGCCAGC TCACATTCTT TGCCACACAT TAAGCAGCAG 
CTGTGGAGCG AAGAATGCTA TCATGGCAAG CTGAGCAGGA AGGCGGCAGA GAGCCTCTTG 
GTAAAGGATG GGGACTTTTT GGTTCGAGAG AGTGCAACAT CCCCTGGCCA ATATGTGCTG 
AGTGGACTAC AGGGAGGCCA AGCAAAACAT CTTCTCCTGG TGGATCCTGA AGGCAAGGTG 
AGGACCAAGG ATCATGTATT TGATAATGTC GGCCACCTTA TCAGATACCA TATGGATAAC 
AGTTTGCCAA TCATCTCCTC TGGAAGCGAA GTAAGCCTTA AACAACCAGT GAGAAAAGAT 
AATAATCCAG CACTTTTGCA TTCCAACAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHC4 ( NM_203349.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_976224.3 Homo sapiens SHC adaptor protein 4 (SHC4), mRNA.

Price & Availability↑

CloneID	OHu07749
Clone ID Related Accession (Same CDS sequence)	NM_203349.3 , NM_203349.4
Accession Version	NM_203349.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	SHC-transforming protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC091073.3, AY464565.1 and AC012379.7. On Nov 23, 2018 this sequence version replaced NM_203349.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.216458.1, SRR1803612.9473.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000332408.9/ ENSP00000329668.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGAGAAC GCGGCCAGGA CAGCCTGGCA GGACTCGTGC TGTATGTAGG ACTCTTCGGG 
CACCCCGGGA TGCTGCACAG GGCCAAGTAC AGCCGCTTTC GGAACGAGTC GATCACGTCC 
TTGGACGAAG GTAGCTCCGG AGGCTCGGTC GGGAACAAGG GCTCGCCGCA GCCTCCCCAC 
CCCGCCCTGG CACCTCACCT GCCGACTGAA GATGCCACCT TGCCGTCGCA GGAGAGCCCC 
ACCCCACTGT GCACCTTGAT CCCCCGCATG GCAAGCATGA AGCTGGCCAA CCCGGCCACT 
TTGCTGAGTC TGAAAAACTT TTGCCTGGGT ACCAAAGAGG TGCCTCGGCT GAAGCTCCAG 
GAAAGCCGGG ACCCAGGTTC CAGCGGCCCC TCTTCCCCAG AAACCAGTTT AAGTAGGTCC 
GGGACTGCAC CTCCACCGCA GCAGGACCTG GTGGGACACA GGGCAACCGC CCTAACCCCT 
GATTCGTGCC CGCTTCCTGG CCCTGGGGAG CCAACACTTA GGAGCAGGCA GGACAGGCAC 
TTTCTACAGC ACCTGTTGGG GATGGGCATG AACTACTGTG TGAGGTACAT GGGCTGTGTT 
GAAGTGCTGC AATCAATGAG ATCACTGGAT TTTGGAATGA GAACCCAAGT TACAAGGGAA 
GCAATAAGTC GCCTGTGTGA AGCTGTCCCC GGGGCAAATG GAGCCATTAA AAAGCGAAAG 
CCTCCAGTTA AGTTCCTATC AACAGTCCTT GGCAAAAGTA ATCTTCAGTT TTCAGGAATG 
AATATAAAAC TGACCATCTC AACATGCAGT CTCACATTGA TGAATCTTGA CAACCAACAG 
ATTATTGCAA ATCATCATAT GCAGTCTATT TCATTTGCCT CTGGAGGGGA TCCTGATACT 
ACAGACTATG TTGCCTACGT AGCTAAAGAT CCAGTTAATC AACGAGCCTG TCACATATTG 
GAATGCCACA ATGGAATGGC CCAAGACGTC ATAAGTACCA TAGGGCAGGC TTTTGAACTC 
CGGTTTAAAC AGTACTTGAA AAATCCTTCT TTGAATACTT CTTGTGAAAG TGAGGAGGTG 
CATATTGATA GCCATGCCGA GGAGAGAGAA GATCATGAAT ATTACAATGA AATTCCAGGG 
AAGCAGCCAC CAGTAGGTGG TGTTTCAGAT ATGCGGATCA AAGTTCAAGC CACGGAACAA 
ATGGCTTACT GCCCCATACA GTGTGAAAAG TTGTGCTATT TGCCTGGAAA CTCCAAGTGC 
AGCAGTGTAT ATGAGAACTG TTTAGAACAA AGCAGGGCAA TAGGTAATGT CCATCCAAGA 
GGGGTGCAGT CCCAGCGAGA TACCTCATTA TTGAAGCACA CGTGCCGAGT GGATCTCTTT 
GATGACCCCT GCTACATTAA TACACAGGCT CTTCAAAGTA CACCTGGCTC TGCTGGAAAT 
CAAAGGTCAG CCCAACCACT GGGGAGCCCA TGGCACTGCG GAAAGGCACC AGAAACTGTT 
CAGCCGGGTG CCACAGCCCA GCCTGCCAGC TCACATTCTT TGCCACACAT TAAGCAGCAG 
CTGTGGAGCG AAGAATGCTA TCATGGCAAG CTGAGCAGGA AGGCGGCAGA GAGCCTCTTG 
GTAAAGGATG GGGACTTTTT GGTTCGAGAG AGTGCAACAT CCCCTGGCCA ATATGTGCTG 
AGTGGACTAC AGGGAGGCCA AGCAAAACAT CTTCTCCTGG TGGATCCTGA AGGCAAGGTG 
AGGACCAAGG ATCATGTATT TGATAATGTC GGCCACCTTA TCAGATACCA TATGGATAAC 
AGTTTGCCAA TCATCTCCTC TGGAAGCGAA GTAAGCCTTA AACAACCAGT GAGAAAAGAT 
AATAATCCAG CACTTTTGCA TTCCAACAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_976224.3
CDS	905..2797
Translation	MRERGQDSLAGLVLYVGLFGHPGMLHRAKYSRFRNESITSLDEGSSGGSVGNKGSPQPPHPALAPHLPTEDATLPSQESPTPLCTLIPRMASMKLANPATLLSLKNFCLGTKEVPRLKLQESRDPGSSGPSSPETSLSRSGTAPPPQQDLVGHRATALTPDSCPLPGPGEPTLRSRQDRHFLQHLLGMGMNYCVRYMGCVEVLQSMRSLDFGMRTQVTREAISRLCEAVPGANGAIKKRKPPVKFLSTVLGKSNLQFSGMNIKLTISTCSLTLMNLDNQQIIANHHMQSISFASGGDPDTTDYVAYVAKDPVNQRACHILECHNGMAQDVISTIGQAFELRFKQYLKNPSLNTSCESEEVHIDSHAEEREDHEYYNEIPGKQPPVGGVSDMRIKVQATEQMAYCPIQCEKLCYLPGNSKCSSVYENCLEQSRAIGNVHPRGVQSQRDTSLLKHTCRVDLFDDPCYINTQALQSTPGSAGNQRSAQPLGSPWHCGKAPETVQPGATAQPASSHSLPHIKQQLWSEECYHGKLSRKAAESLLVKDGDFLVRESATSPGQYVLSGLQGGQAKHLLLVDPEGKVRTKDHVFDNVGHLIRYHMDNSLPIISSGSEVSLKQPVRKDNNPALLHSNK

Target ORF information:

RefSeq Version	NM_203349.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SHC adaptor protein 4 (SHC4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_203349.4

ORF Insert Sequence:

ATGCGAGAAC GCGGCCAGGA CAGCCTGGCA GGACTCGTGC TGTATGTAGG ACTCTTCGGG 
CACCCCGGGA TGCTGCACAG GGCCAAGTAC AGCCGCTTTC GGAACGAGTC GATCACGTCC 
TTGGACGAAG GTAGCTCCGG AGGCTCGGTC GGGAACAAGG GCTCGCCGCA GCCTCCCCAC 
CCCGCCCTGG CACCTCACCT GCCGACTGAA GATGCCACCT TGCCGTCGCA GGAGAGCCCC 
ACCCCACTGT GCACCTTGAT CCCCCGCATG GCAAGCATGA AGCTGGCCAA CCCGGCCACT 
TTGCTGAGTC TGAAAAACTT TTGCCTGGGT ACCAAAGAGG TGCCTCGGCT GAAGCTCCAG 
GAAAGCCGGG ACCCAGGTTC CAGCGGCCCC TCTTCCCCAG AAACCAGTTT AAGTAGGTCC 
GGGACTGCAC CTCCACCGCA GCAGGACCTG GTGGGACACA GGGCAACCGC CCTAACCCCT 
GATTCGTGCC CGCTTCCTGG CCCTGGGGAG CCAACACTTA GGAGCAGGCA GGACAGGCAC 
TTTCTACAGC ACCTGTTGGG GATGGGCATG AACTACTGTG TGAGGTACAT GGGCTGTGTT 
GAAGTGCTGC AATCAATGAG ATCACTGGAT TTTGGAATGA GAACCCAAGT TACAAGGGAA 
GCAATAAGTC GCCTGTGTGA AGCTGTCCCC GGGGCAAATG GAGCCATTAA AAAGCGAAAG 
CCTCCAGTTA AGTTCCTATC AACAGTCCTT GGCAAAAGTA ATCTTCAGTT TTCAGGAATG 
AATATAAAAC TGACCATCTC AACATGCAGT CTCACATTGA TGAATCTTGA CAACCAACAG 
ATTATTGCAA ATCATCATAT GCAGTCTATT TCATTTGCCT CTGGAGGGGA TCCTGATACT 
ACAGACTATG TTGCCTACGT AGCTAAAGAT CCAGTTAATC AACGAGCCTG TCACATATTG 
GAATGCCACA ATGGAATGGC CCAAGACGTC ATAAGTACCA TAGGGCAGGC TTTTGAACTC 
CGGTTTAAAC AGTACTTGAA AAATCCTTCT TTGAATACTT CTTGTGAAAG TGAGGAGGTG 
CATATTGATA GCCATGCCGA GGAGAGAGAA GATCATGAAT ATTACAATGA AATTCCAGGG 
AAGCAGCCAC CAGTAGGTGG TGTTTCAGAT ATGCGGATCA AAGTTCAAGC CACGGAACAA 
ATGGCTTACT GCCCCATACA GTGTGAAAAG TTGTGCTATT TGCCTGGAAA CTCCAAGTGC 
AGCAGTGTAT ATGAGAACTG TTTAGAACAA AGCAGGGCAA TAGGTAATGT CCATCCAAGA 
GGGGTGCAGT CCCAGCGAGA TACCTCATTA TTGAAGCACA CGTGCCGAGT GGATCTCTTT 
GATGACCCCT GCTACATTAA TACACAGGCT CTTCAAAGTA CACCTGGCTC TGCTGGAAAT 
CAAAGGTCAG CCCAACCACT GGGGAGCCCA TGGCACTGCG GAAAGGCACC AGAAACTGTT 
CAGCCGGGTG CCACAGCCCA GCCTGCCAGC TCACATTCTT TGCCACACAT TAAGCAGCAG 
CTGTGGAGCG AAGAATGCTA TCATGGCAAG CTGAGCAGGA AGGCGGCAGA GAGCCTCTTG 
GTAAAGGATG GGGACTTTTT GGTTCGAGAG AGTGCAACAT CCCCTGGCCA ATATGTGCTG 
AGTGGACTAC AGGGAGGCCA AGCAAAACAT CTTCTCCTGG TGGATCCTGA AGGCAAGGTG 
AGGACCAAGG ATCATGTATT TGATAATGTC GGCCACCTTA TCAGATACCA TATGGATAAC 
AGTTTGCCAA TCATCTCCTC TGGAAGCGAA GTAAGCCTTA AACAACCAGT GAGAAAAGAT 
AATAATCCAG CACTTTTGCA TTCCAACAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SHC4 ( XM_005254375.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254432.1 Homo sapiens SHC adaptor protein 4 (SHC4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu48289
Clone ID Related Accession (Same CDS sequence)	XM_005254375.3
Accession Version	XM_005254375.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	SHC-transforming protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254375.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGACA AATCTCCAAA TTTCTCTTCA GAAAATTACA TGGGCTGTGT TGAAGTGCTG 
CAATCAATGA GATCACTGGA TTTTGGAATG AGAACCCAAG TTACAAGGGA AGCAATAAGT 
CGCCTGTGTG AAGCTGTCCC CGGGGCAAAT GGAGCCATTA AAAAGCGAAA GCCTCCAGTT 
AAGTTCCTAT CAACAGTCCT TGGCAAAAGT AATCTTCAGT TTTCAGGAAT GAATATAAAA 
CTGACCATCT CAACATGCAG TCTCACATTG ATGAATCTTG ACAACCAACA GATTATTGCA 
AATCATCATA TGCAGTCTAT TTCATTTGCC TCTGGAGGGG ATCCTGATAC TACAGACTAT 
GTTGCCTACG TAGCTAAAGA TCCAGTTAAT CAACGAGCCT GTCACATATT GGAATGCCAC 
AATGGAATGG CCCAAGACGT CATAAGTACC ATAGGGCAGG CTTTTGAACT CCGGTTTAAA 
CAGTACTTGA AAAATCCTTC TTTGAATACT TCTTGTGAAA GTGAGGAGGT GCATATTGAT 
AGCCATGCCG AGGAGAGAGA AGATCATGAA TATTACAATG AAATTCCAGG GAAGCAGCCA 
CCAGTAGGTG GTGTTTCAGA TATGCGGATC AAAGTTCAAG CCACGGAACA AATGGCTTAC 
TGCCCCATAC AGTGTGAAAA GTTGTGCTAT TTGCCTGGAA ACTCCAAGTG CAGCAGTGTA 
TATGAGAACT GTTTAGAACA AAGCAGGGCA ATAGGTAATG TCCATCCAAG AGGGGTGCAG 
TCCCAGCGAG ATACCTCATT ATTGAAGCAC ACGTGCCGAG TGGATCTCTT TGATGACCCC 
TGCTACATTA ATACACAGGC TCTTCAAAGT ACACCTGGCT CTGCTGGAAA TCAAAGGTCA 
GCCCAACCAC TGGGGAGCCC ATGGCACTGC GGAAAGGCAC CAGAAACTGT TCAGCCGGGT 
GCCACAGCCC AGCCTGCCAG CTCACATTCT TTGCCACACA TTAAGCAGCA GCTGTGGAGC 
GAAGAATGCT ATCATGGCAA GCTGAGCAGG AAGGCGGCAG AGAGCCTCTT GGTAAAGGAT 
GGGGACTTTT TGGTTCGAGA GAGTGCAACA TCCCCTGGCC AATATGTGCT GAGTGGACTA 
CAGGGAGGCC AAGCAAAACA TCTTCTCCTG GTGGATCCTG AAGGCAAGGT GAGGACCAAG 
GATCATGTAT TTGATAATGT CGGCCACCTT ATCAGATACC ATATGGATAA CAGTTTGCCA 
ATCATCTCCT CTGGAAGCGA AGTAAGCCTT AAACAACCAG TGAGAAAAGA TAATAATCCA 
GCACTTTTGC ATTCCAACAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254432.1
CDS	136..1479
Translation	MCDKSPNFSSENYMGCVEVLQSMRSLDFGMRTQVTREAISRLCEAVPGANGAIKKRKPPVKFLSTVLGKSNLQFSGMNIKLTISTCSLTLMNLDNQQIIANHHMQSISFASGGDPDTTDYVAYVAKDPVNQRACHILECHNGMAQDVISTIGQAFELRFKQYLKNPSLNTSCESEEVHIDSHAEEREDHEYYNEIPGKQPPVGGVSDMRIKVQATEQMAYCPIQCEKLCYLPGNSKCSSVYENCLEQSRAIGNVHPRGVQSQRDTSLLKHTCRVDLFDDPCYINTQALQSTPGSAGNQRSAQPLGSPWHCGKAPETVQPGATAQPASSHSLPHIKQQLWSEECYHGKLSRKAAESLLVKDGDFLVRESATSPGQYVLSGLQGGQAKHLLLVDPEGKVRTKDHVFDNVGHLIRYHMDNSLPIISSGSEVSLKQPVRKDNNPALLHSNK

Target ORF information:

RefSeq Version	XM_005254375.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SHC adaptor protein 4 (SHC4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254375.3

ORF Insert Sequence:

ATGTGTGACA AATCTCCAAA TTTCTCTTCA GAAAATTACA TGGGCTGTGT TGAAGTGCTG 
CAATCAATGA GATCACTGGA TTTTGGAATG AGAACCCAAG TTACAAGGGA AGCAATAAGT 
CGCCTGTGTG AAGCTGTCCC CGGGGCAAAT GGAGCCATTA AAAAGCGAAA GCCTCCAGTT 
AAGTTCCTAT CAACAGTCCT TGGCAAAAGT AATCTTCAGT TTTCAGGAAT GAATATAAAA 
CTGACCATCT CAACATGCAG TCTCACATTG ATGAATCTTG ACAACCAACA GATTATTGCA 
AATCATCATA TGCAGTCTAT TTCATTTGCC TCTGGAGGGG ATCCTGATAC TACAGACTAT 
GTTGCCTACG TAGCTAAAGA TCCAGTTAAT CAACGAGCCT GTCACATATT GGAATGCCAC 
AATGGAATGG CCCAAGACGT CATAAGTACC ATAGGGCAGG CTTTTGAACT CCGGTTTAAA 
CAGTACTTGA AAAATCCTTC TTTGAATACT TCTTGTGAAA GTGAGGAGGT GCATATTGAT 
AGCCATGCCG AGGAGAGAGA AGATCATGAA TATTACAATG AAATTCCAGG GAAGCAGCCA 
CCAGTAGGTG GTGTTTCAGA TATGCGGATC AAAGTTCAAG CCACGGAACA AATGGCTTAC 
TGCCCCATAC AGTGTGAAAA GTTGTGCTAT TTGCCTGGAA ACTCCAAGTG CAGCAGTGTA 
TATGAGAACT GTTTAGAACA AAGCAGGGCA ATAGGTAATG TCCATCCAAG AGGGGTGCAG 
TCCCAGCGAG ATACCTCATT ATTGAAGCAC ACGTGCCGAG TGGATCTCTT TGATGACCCC 
TGCTACATTA ATACACAGGC TCTTCAAAGT ACACCTGGCT CTGCTGGAAA TCAAAGGTCA 
GCCCAACCAC TGGGGAGCCC ATGGCACTGC GGAAAGGCAC CAGAAACTGT TCAGCCGGGT 
GCCACAGCCC AGCCTGCCAG CTCACATTCT TTGCCACACA TTAAGCAGCA GCTGTGGAGC 
GAAGAATGCT ATCATGGCAA GCTGAGCAGG AAGGCGGCAG AGAGCCTCTT GGTAAAGGAT 
GGGGACTTTT TGGTTCGAGA GAGTGCAACA TCCCCTGGCC AATATGTGCT GAGTGGACTA 
CAGGGAGGCC AAGCAAAACA TCTTCTCCTG GTGGATCCTG AAGGCAAGGT GAGGACCAAG 
GATCATGTAT TTGATAATGT CGGCCACCTT ATCAGATACC ATATGGATAA CAGTTTGCCA 
ATCATCTCCT CTGGAAGCGA AGTAAGCCTT AAACAACCAG TGAGAAAAGA TAATAATCCA 
GCACTTTTGC ATTCCAACAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The ShcD phosphotyrosine adaptor subverts canonical EGF receptor trafficking.
Journal of cell science130(17)2808-2820(2017 Sep)
Wills MKB,Lau HR,Jones N

Signaling adaptor ShcD suppresses extracellular signal-regulated kinase (Erk) phosphorylation distal to the Ret and Trk neurotrophic receptors.
The Journal of biological chemistry292(14)5748-5759(2017 04)
Wills MK,Keyvani Chahi A,Lau HR,Tilak M,Guild BD,New LA,Lu P,Jacquet K,Meakin SO,Bisson N,Jones N

Enhanced prediction of Src homology 2 (SH2) domain binding potentials using a fluorescence polarization-derived c-Met, c-Kit, ErbB, and androgen receptor interactome.
Molecular & cellular proteomics : MCP13(7)1705-23(2014 Jul)
Leung KK,Hause RJ Jr,Barkinge JL,Ciaccio MF,Chuu CP,Jones RB

The mammalian-membrane two-hybrid assay (MaMTH) for probing membrane-protein interactions in human cells.
Nature methods11(5)585-92(2014 May)
Petschnigg J,Groisman B,Kotlyar M,Taipale M,Zheng Y,Kurat CF,Sayad A,Sierra JR,Mattiazzi Usaj M,Snider J,Nachman A,Krykbaeva I,Tsao MS,Moffat J,Pawson T,Lindquist S,Jurisica I,Stagljar I

The ShcD signaling adaptor facilitates ligand-independent phosphorylation of the EGF receptor.
Molecular biology of the cell25(6)739-52(2014 Mar)
Wills MK,Tong J,Tremblay SL,Moran MF,Jones N

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SHC4 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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