ARL1 cDNA ORF clone, Homo sapiens(human)
| Gene Symbol | ARL1 |
| Entrez Gene ID | 400 |
| Full Name | ADP ribosylation factor like GTPase 1 |
| Synonyms | ARFL1 |
| General protein information |
|
| Gene Type | protein-coding |
| Organism | Homo sapiens(human) |
| Genome | |
| Summary | The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]. |
| Disorder MIM: |
mRNA and Protein(s)
| mRNA | Protein | Name |
|---|---|---|
| NM_001177.5 | NP_001168.1 | ADP-ribosylation factor-like protein 1 isoform 1 |
| NM_001301068.1 | NP_001287997.1 | ADP-ribosylation factor-like protein 1 isoform 2 |
| NM_001177.6 | NP_001168.1 | ADP-ribosylation factor-like protein 1 isoform 1 |
| Homo sapiens (human) | ARL1 | NP_001168.1 |
| Caenorhabditis elegans (roundworm) | arl-1 | NP_495816.1 |
| Saccharomyces cerevisiae (baker's yeast) | ARL1 | NP_009723.3 |
| Kluyveromyces lactis | KLLA0E24773g | XP_455068.1 |
| Drosophila melanogaster (fruit fly) | Arf72A | NP_524098.2 |
| Anopheles gambiae (African malaria mosquito) | AgaP_AGAP011730 | XP_320779.3 |
| Arabidopsis thaliana (thale cress) | ARF3 | NP_850057.1 |
| Canis lupus familiaris (dog) | ARL1 | XP_852990.1 |
| Mus musculus (house mouse) | Arl1 | NP_080135.2 |
| Rattus norvegicus (Norway rat) | Arl1 | NP_071780.1 |
| Eremothecium gossypii | AGOS_AGR221W | NP_986887.2 |
| Neurospora crassa | NCU08989 | XP_959017.1 |
| Pan troglodytes (chimpanzee) | ARL1 | XP_509308.2 |
| Bos taurus (cattle) | ARL1 | NP_001039694.1 |
| Gallus gallus (chicken) | ARL1 | NP_001074963.1 |
| Danio rerio (zebrafish) | arl1 | NP_001002473.1 |
| Magnaporthe oryzae (rice blast fungus) | MGG_04976 | XP_003712475.1 |
| Xenopus tropicalis (tropical clawed frog) | arl1 | NP_001016396.1 |
| Oryza sativa (rice) | Os08g0248900 | NP_001061368.1 |
Related articles in PubMed
Structural Insights into Arl1-Mediated Targeting of the Arf-GEF BIG1 to the trans-Golgi.
Galindo A, Soler N, McLaughlin SH, Yu M, Williams RL, Munro S
Cell reports16(3)839-50(2016 07)
Genome-wide data reveal novel genes for methotrexate response in a large cohort of juvenile idiopathic arthritis cases.
Cobb J, Cule E, Moncrieffe H, Hinks A, Ursu S, Patrick F, Kassoumeri L, Flynn E, Bulatovi? M, Wulffraat N, van Zelst B, de Jonge R, Bohm M, Dolezalova P, Hirani S, Newman S, Whitworth P, Southwood TR, De Iorio M, , , , Wedderburn LR, Thomson W
The pharmacogenomics journal14(4)356-64(2014 Aug)
Rab4 orchestrates a small GTPase cascade for recruitment of adaptor proteins to early endosomes.
D'Souza RS, Semus R, Billings EA, Meyer CB, Conger K, Casanova JE
Current biology : CB24(11)1187-98(2014 Jun)
Arl1p regulates spatial membrane organization at the trans-Golgi network through interaction with Arf-GEF Gea2p and flippase Drs2p.
Tsai PC, Hsu JW, Liu YW, Chen KY, Lee FJ
Proceedings of the National Academy of Sciences of the United States of America110(8)E668-77(2013 Feb)
Structural basis for membrane binding specificity of the Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-2 determined by Arl1 GTPase.
Nakamura K, Man Z, Xie Y, Hanai A, Makyio H, Kawasaki M, Kato R, Shin HW, Nakayama K, Wakatsuki S
The Journal of biological chemistry287(30)25478-89(2012 Jul)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Plasticity allows Arl1 to interact with different effectors of unrelated structure.
Title: Structural Insights into Arl1-Mediated Targeting of the Arf-GEF BIG1 to the trans-Golgi.
Together the findings indicate that Arl1 links Rab4-dependent formation of endosomal sorting domains with downstream assembly of adaptor protein complexes that constitute the endosomal sorting machinery.
Title: Rab4 orchestrates a small GTPase cascade for recruitment of adaptor proteins to early endosomes.
results demonstrate that activated Arl1p can promote the spatial modulation of membrane organization at the trans-Golgi network through interacting with the effectors Gea2p and Drs2p
Title: Arl1p regulates spatial membrane organization at the trans-Golgi network through interaction with Arf-GEF Gea2p and flippase Drs2p.
The Arl1.Arfaptin-2 BAR structure suggests that one of the two Arl1 molecules competes with Rac1, which binds to the concave face of the Arfaptin-2 BAR homodimer and may hinder its membrane association.
Title: Structural basis for membrane binding specificity of the Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-2 determined by Arl1 GTPase.
arfaptins are recruited onto trans-Golgi membranes by interacting with Arl1, and capable of inducing membrane deformation via their BAR domains.
Title: Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs.
The following ARL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu02804 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_001177.5 , NM_001177.6 | |
| Accession Version | NM_001177.5 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | ADP-ribosylation factor-like protein 1 isoform 1 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC343685.1, BX537387.1, AC063948.44 and AI802608.1. On Jul 19, 2014 this sequence version replaced NM_001177.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.62661.1, SRR1803612.267399.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2151358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGGTGGCT TTTTCTCAAG TATATTTTCC AGTCTGTTTG GAACTCGGGA AATGAGAATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001168.1 |
| CDS | 176..721 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001177.5 |
ORF Insert Sequence:
1 | ATGGGTGGCT TTTTCTCAAG TATATTTTCC AGTCTGTTTG GAACTCGGGA AATGAGAATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu02804 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_001177.5 , NM_001177.6 | |
| Accession Version | NM_001177.6 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 546bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-06-07 | |
| Organism | Homo sapiens(human) | |
| Product | ADP-ribosylation factor-like protein 1 isoform 1 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX537387.1, AC063948.44 and AI802608.1. On Nov 22, 2018 this sequence version replaced NM_001177.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.53922.1, SRR1803616.185226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2151358 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261636.13/ ENSP00000261636.8 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. | |
1 | ATGGGTGGCT TTTTCTCAAG TATATTTTCC AGTCTGTTTG GAACTCGGGA AATGAGAATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001168.1 |
| CDS | 128..673 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001177.6 |
ORF Insert Sequence:
1 | ATGGGTGGCT TTTTCTCAAG TATATTTTCC AGTCTGTTTG GAACTCGGGA AATGAGAATT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu46552 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001301068.1 | |
| Accession Version | NM_001301068.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 408bp) Protein sequence SNP |
|
| Vector | pcDNA3.1+/C-(K)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-06-07 | |
| Organism | Homo sapiens(human) | |
| Product | ADP-ribosylation factor-like protein 1 isoform 2 | |
| Comment | Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC343685.1, AK302911.1, AC063948.44 and AI802608.1. On Jul 19, 2014 this sequence version replaced XM_005268869.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.282391.1, SRR1660803.44562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467147 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGCCATTG GATTTAATGT AGAGACGGTG ACGTACAAAA ACCTTAAATT CCAAGTCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001287997.1 |
| CDS | 176..583 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001301068.1 |
ORF Insert Sequence:
1 | ATGGCCATTG GATTTAATGT AGAGACGGTG ACGTACAAAA ACCTTAAATT CCAAGTCTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
 |
Structural Insights into Arl1-Mediated Targeting of the Arf-GEF BIG1 to the trans-Golgi. |
|
Genome-wide data reveal novel genes for methotrexate response in a large cohort of juvenile idiopathic arthritis cases. |
|
Rab4 orchestrates a small GTPase cascade for recruitment of adaptor proteins to early endosomes. |
|
Arl1p regulates spatial membrane organization at the trans-Golgi network through interaction with Arf-GEF Gea2p and flippase Drs2p. |
|
Structural basis for membrane binding specificity of the Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-2 determined by Arl1 GTPase. |