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ARL1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol ARL1
Entrez Gene ID 400
Full Name ADP ribosylation factor like GTPase 1
Synonyms ARFL1
General protein information
Preferred Names

ADP ribosylation factor like GTPase 1

Names

ADP-ribosylation factor-like protein 1
ADP-ribosylation factor-like 1
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

12

12q23.2
Summary The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014].
Disorder MIM:

603425


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001177.5 NP_001168.1 ADP-ribosylation factor-like protein 1 isoform 1
NM_001301068.1 NP_001287997.1 ADP-ribosylation factor-like protein 1 isoform 2
NM_001177.6 NP_001168.1 ADP-ribosylation factor-like protein 1 isoform 1

Pathways from BioSystems


Homologies


Homo sapiens (human) ARL1 NP_001168.1
Caenorhabditis elegans (roundworm) arl-1 NP_495816.1
Saccharomyces cerevisiae (baker's yeast) ARL1 NP_009723.3
Kluyveromyces lactis KLLA0E24773g XP_455068.1
Drosophila melanogaster (fruit fly) Arf72A NP_524098.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP011730 XP_320779.3
Arabidopsis thaliana (thale cress) ARF3 NP_850057.1
Canis lupus familiaris (dog) ARL1 XP_852990.1
Mus musculus (house mouse) Arl1 NP_080135.2
Rattus norvegicus (Norway rat) Arl1 NP_071780.1
Eremothecium gossypii AGOS_AGR221W NP_986887.2
Neurospora crassa NCU08989 XP_959017.1
Pan troglodytes (chimpanzee) ARL1 XP_509308.2
Bos taurus (cattle) ARL1 NP_001039694.1
Gallus gallus (chicken) ARL1 NP_001074963.1
Danio rerio (zebrafish) arl1 NP_001002473.1
Magnaporthe oryzae (rice blast fungus) MGG_04976 XP_003712475.1
Xenopus tropicalis (tropical clawed frog) arl1 NP_001016396.1
Oryza sativa (rice) Os08g0248900 NP_001061368.1

Gene Ontology


Bibliography

Related articles in PubMed

Structural Insights into Arl1-Mediated Targeting of the Arf-GEF BIG1 to the trans-Golgi.
Galindo A, Soler N, McLaughlin SH, Yu M, Williams RL, Munro S
Cell reports16(3)839-50(2016 07)

Genome-wide data reveal novel genes for methotrexate response in a large cohort of juvenile idiopathic arthritis cases.
Cobb J, Cule E, Moncrieffe H, Hinks A, Ursu S, Patrick F, Kassoumeri L, Flynn E, Bulatovi? M, Wulffraat N, van Zelst B, de Jonge R, Bohm M, Dolezalova P, Hirani S, Newman S, Whitworth P, Southwood TR, De Iorio M, , , , Wedderburn LR, Thomson W
The pharmacogenomics journal14(4)356-64(2014 Aug)

Rab4 orchestrates a small GTPase cascade for recruitment of adaptor proteins to early endosomes.
D'Souza RS, Semus R, Billings EA, Meyer CB, Conger K, Casanova JE
Current biology : CB24(11)1187-98(2014 Jun)

Arl1p regulates spatial membrane organization at the trans-Golgi network through interaction with Arf-GEF Gea2p and flippase Drs2p.
Tsai PC, Hsu JW, Liu YW, Chen KY, Lee FJ
Proceedings of the National Academy of Sciences of the United States of America110(8)E668-77(2013 Feb)

Structural basis for membrane binding specificity of the Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-2 determined by Arl1 GTPase.
Nakamura K, Man Z, Xie Y, Hanai A, Makyio H, Kawasaki M, Kato R, Shin HW, Nakayama K, Wakatsuki S
The Journal of biological chemistry287(30)25478-89(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Plasticity allows Arl1 to interact with different effectors of unrelated structure.
Title: Structural Insights into Arl1-Mediated Targeting of the Arf-GEF BIG1 to the trans-Golgi.

Together the findings indicate that Arl1 links Rab4-dependent formation of endosomal sorting domains with downstream assembly of adaptor protein complexes that constitute the endosomal sorting machinery.
Title: Rab4 orchestrates a small GTPase cascade for recruitment of adaptor proteins to early endosomes.

results demonstrate that activated Arl1p can promote the spatial modulation of membrane organization at the trans-Golgi network through interacting with the effectors Gea2p and Drs2p
Title: Arl1p regulates spatial membrane organization at the trans-Golgi network through interaction with Arf-GEF Gea2p and flippase Drs2p.

The Arl1.Arfaptin-2 BAR structure suggests that one of the two Arl1 molecules competes with Rac1, which binds to the concave face of the Arfaptin-2 BAR homodimer and may hinder its membrane association.
Title: Structural basis for membrane binding specificity of the Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-2 determined by Arl1 GTPase.

arfaptins are recruited onto trans-Golgi membranes by interacting with Arl1, and capable of inducing membrane deformation via their BAR domains.
Title: Arfaptins are localized to the trans-Golgi by interaction with Arl1, but not Arfs.

The following ARL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu02804 NM_001177.6
Latest version!		 Homo sapiens ADP ribosylation factor like GTPase 1 (ARL1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu46552 NM_001301068.1
Latest version!		 Homo sapiens ADP ribosylation factor like GTPase 1 (ARL1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
View Old Accession Versions >>
OHu02804 NM_001177.5 Homo sapiens ADP ribosylation factor like GTPase 1 (ARL1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu02804
Clone ID Related Accession (Same CDS sequence) NM_001177.5 , NM_001177.6
Accession Version NM_001177.5 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 546bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-05
Organism Homo sapiens(human)
Product ADP-ribosylation factor-like protein 1 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC343685.1, BX537387.1, AC063948.44 and AI802608.1. On Jul 19, 2014 this sequence version replaced NM_001177.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.62661.1, SRR1803612.267399.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2151358 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
ATGGGTGGCT TTTTCTCAAG TATATTTTCC AGTCTGTTTG GAACTCGGGA AATGAGAATT 
TTAATTTTGG GATTAGATGG AGCAGGAAAA ACCACAATTT TGTACAGATT ACAAGTGGGA 
GAAGTTGTTA CTACTATACC TACCATTGGA TTTAATGTAG AGACGGTGAC GTACAAAAAC 
CTTAAATTCC AAGTCTGGGA TTTAGGAGGA CAGACAAGTA TCAGGCCATA CTGGAGATGT 
TACTATTCAA ACACAGATGC AGTCATTTAT GTAGTAGACA GTTGTGACCG AGACCGAATT 
GGCATTTCCA AATCAGAGTT AGTTGCCATG TTGGAGGAAG AAGAGCTGAG AAAAGCCATT 
TTAGTGGTGT TTGCAAATAA ACAGGACATG GAACAGGCCA TGACTTCCTC AGAGATGGCA 
AATTCACTTG GGTTACCTGC CTTGAAGGAC CGAAAATGGC AGATATTCAA AACGTCAGCA 
ACCAAAGGCA CCGGCCTTGA TGAGGCAATG GAATGGTTAG TTGAAACATT AAAAAGCAGA 
CAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001168.1
CDS176..721
Translation

Target ORF information:

RefSeq Version NM_001177.5
Organism Homo sapiens(human)
Definition Homo sapiens ADP ribosylation factor like GTPase 1 (ARL1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001177.5

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGTGGCT TTTTCTCAAG TATATTTTCC AGTCTGTTTG GAACTCGGGA AATGAGAATT 
TTAATTTTGG GATTAGATGG AGCAGGAAAA ACCACAATTT TGTACAGATT ACAAGTGGGA 
GAAGTTGTTA CTACTATACC TACCATTGGA TTTAATGTAG AGACGGTGAC GTACAAAAAC 
CTTAAATTCC AAGTCTGGGA TTTAGGAGGA CAGACAAGTA TCAGGCCATA CTGGAGATGT 
TACTATTCAA ACACAGATGC AGTCATTTAT GTAGTAGACA GTTGTGACCG AGACCGAATT 
GGCATTTCCA AATCAGAGTT AGTTGCCATG TTGGAGGAAG AAGAGCTGAG AAAAGCCATT 
TTAGTGGTGT TTGCAAATAA ACAGGACATG GAACAGGCCA TGACTTCCTC AGAGATGGCA 
AATTCACTTG GGTTACCTGC CTTGAAGGAC CGAAAATGGC AGATATTCAA AACGTCAGCA 
ACCAAAGGCA CCGGCCTTGA TGAGGCAATG GAATGGTTAG TTGAAACATT AAAAAGCAGA 
CAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02804
Clone ID Related Accession (Same CDS sequence) NM_001177.5 , NM_001177.6
Accession Version NM_001177.6 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 546bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-11-16
Organism Homo sapiens(human)
Product ADP-ribosylation factor-like protein 1 isoform 1
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX537387.1, AC063948.44 and AI802608.1. On Nov 22, 2018 this sequence version replaced NM_001177.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.53922.1, SRR1803616.185226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2151358 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261636.13/ ENSP00000261636.8 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
ATGGGTGGCT TTTTCTCAAG TATATTTTCC AGTCTGTTTG GAACTCGGGA AATGAGAATT 
TTAATTTTGG GATTAGATGG AGCAGGAAAA ACCACAATTT TGTACAGATT ACAAGTGGGA 
GAAGTTGTTA CTACTATACC TACCATTGGA TTTAATGTAG AGACGGTGAC GTACAAAAAC 
CTTAAATTCC AAGTCTGGGA TTTAGGAGGA CAGACAAGTA TCAGGCCATA CTGGAGATGT 
TACTATTCAA ACACAGATGC AGTCATTTAT GTAGTAGACA GTTGTGACCG AGACCGAATT 
GGCATTTCCA AATCAGAGTT AGTTGCCATG TTGGAGGAAG AAGAGCTGAG AAAAGCCATT 
TTAGTGGTGT TTGCAAATAA ACAGGACATG GAACAGGCCA TGACTTCCTC AGAGATGGCA 
AATTCACTTG GGTTACCTGC CTTGAAGGAC CGAAAATGGC AGATATTCAA AACGTCAGCA 
ACCAAAGGCA CCGGCCTTGA TGAGGCAATG GAATGGTTAG TTGAAACATT AAAAAGCAGA 
CAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001168.1
CDS128..673
Translation

Target ORF information:

RefSeq Version NM_001177.6
Organism Homo sapiens(human)
Definition Homo sapiens ADP ribosylation factor like GTPase 1 (ARL1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001177.6

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGGTGGCT TTTTCTCAAG TATATTTTCC AGTCTGTTTG GAACTCGGGA AATGAGAATT 
TTAATTTTGG GATTAGATGG AGCAGGAAAA ACCACAATTT TGTACAGATT ACAAGTGGGA 
GAAGTTGTTA CTACTATACC TACCATTGGA TTTAATGTAG AGACGGTGAC GTACAAAAAC 
CTTAAATTCC AAGTCTGGGA TTTAGGAGGA CAGACAAGTA TCAGGCCATA CTGGAGATGT 
TACTATTCAA ACACAGATGC AGTCATTTAT GTAGTAGACA GTTGTGACCG AGACCGAATT 
GGCATTTCCA AATCAGAGTT AGTTGCCATG TTGGAGGAAG AAGAGCTGAG AAAAGCCATT 
TTAGTGGTGT TTGCAAATAA ACAGGACATG GAACAGGCCA TGACTTCCTC AGAGATGGCA 
AATTCACTTG GGTTACCTGC CTTGAAGGAC CGAAAATGGC AGATATTCAA AACGTCAGCA 
ACCAAAGGCA CCGGCCTTGA TGAGGCAATG GAATGGTTAG TTGAAACATT AAAAAGCAGA 
CAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu46552
Clone ID Related Accession (Same CDS sequence) NM_001301068.1
Accession Version NM_001301068.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 408bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-06-07
Organism Homo sapiens(human)
Product ADP-ribosylation factor-like protein 1 isoform 2
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC343685.1, AK302911.1, AC063948.44 and AI802608.1. On Jul 19, 2014 this sequence version replaced XM_005268869.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.282391.1, SRR1660803.44562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467147 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
ATGGCCATTG GATTTAATGT AGAGACGGTG ACGTACAAAA ACCTTAAATT CCAAGTCTGG 
GATTTAGGAG GACAGACAAG TATCAGGCCA TACTGGAGAT GTTACTATTC AAACACAGAT 
GCAGTCATTT ATGTAGTAGA CAGTTGTGAC CGAGACCGAA TTGGCATTTC CAAATCAGAG 
TTAGTTGCCA TGTTGGAGGA AGAAGAGCTG AGAAAAGCCA TTTTAGTGGT GTTTGCAAAT 
AAACAGGACA TGGAACAGGC CATGACTTCC TCAGAGATGG CAAATTCACT TGGGTTACCT 
GCCTTGAAGG ACCGAAAATG GCAGATATTC AAAACGTCAG CAACCAAAGG CACCGGCCTT 
GATGAGGCAA TGGAATGGTT AGTTGAAACA TTAAAAAGCA GACAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001287997.1
CDS176..583
Translation

Target ORF information:

RefSeq Version NM_001301068.1
Organism Homo sapiens(human)
Definition Homo sapiens ADP ribosylation factor like GTPase 1 (ARL1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001301068.1

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGCCATTG GATTTAATGT AGAGACGGTG ACGTACAAAA ACCTTAAATT CCAAGTCTGG 
GATTTAGGAG GACAGACAAG TATCAGGCCA TACTGGAGAT GTTACTATTC AAACACAGAT 
GCAGTCATTT ATGTAGTAGA CAGTTGTGAC CGAGACCGAA TTGGCATTTC CAAATCAGAG 
TTAGTTGCCA TGTTGGAGGA AGAAGAGCTG AGAAAAGCCA TTTTAGTGGT GTTTGCAAAT 
AAACAGGACA TGGAACAGGC CATGACTTCC TCAGAGATGG CAAATTCACT TGGGTTACCT 
GCCTTGAAGG ACCGAAAATG GCAGATATTC AAAACGTCAG CAACCAAAGG CACCGGCCTT 
GATGAGGCAA TGGAATGGTT AGTTGAAACA TTAAAAAGCA GACAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Structural Insights into Arl1-Mediated Targeting of the Arf-GEF BIG1 to the trans-Golgi.
Cell reports16(3)839-50(2016 07)
Galindo A,Soler N,McLaughlin SH,Yu M,Williams RL,Munro S


book

Genome-wide data reveal novel genes for methotrexate response in a large cohort of juvenile idiopathic arthritis cases.
The pharmacogenomics journal14(4)356-64(2014 Aug)
Cobb J,Cule E,Moncrieffe H,Hinks A,Ursu S,Patrick F,Kassoumeri L,Flynn E,Bulatovi? M,Wulffraat N,van Zelst B,de Jonge R,Bohm M,Dolezalova P,Hirani S,Newman S,Whitworth P,Southwood TR,De Iorio M,,,,Wedderburn LR,Thomson W


book

Rab4 orchestrates a small GTPase cascade for recruitment of adaptor proteins to early endosomes.
Current biology : CB24(11)1187-98(2014 Jun)
D'Souza RS,Semus R,Billings EA,Meyer CB,Conger K,Casanova JE


book

Arl1p regulates spatial membrane organization at the trans-Golgi network through interaction with Arf-GEF Gea2p and flippase Drs2p.
Proceedings of the National Academy of Sciences of the United States of America110(8)E668-77(2013 Feb)
Tsai PC,Hsu JW,Liu YW,Chen KY,Lee FJ


book

Structural basis for membrane binding specificity of the Bin/Amphiphysin/Rvs (BAR) domain of Arfaptin-2 determined by Arl1 GTPase.
The Journal of biological chemistry287(30)25478-89(2012 Jul)
Nakamura K,Man Z,Xie Y,Hanai A,Makyio H,Kawasaki M,Kato R,Shin HW,Nakayama K,Wakatsuki S