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MAD2L1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MAD2L1
Entrez Gene ID 4085
Full Name mitotic arrest deficient 2 like 1
Synonyms HSMAD2,MAD2
General protein information
Preferred Names

mitotic arrest deficient 2 like 1

Names

mitotic spindle assembly checkpoint protein MAD2A
MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
MAD2 mitotic arrest deficient-like 1
MAD2-like protein 1
mitotic arrest deficient 2-like protein 1
mitotic arrest deficient, yeast, homolog-like 1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

4

4q27

Summary MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008].
Disorder MIM:

601467


mRNA and Protein(s)

mRNA Protein Name
NM_002358.3 NP_002349.1 mitotic spindle assembly checkpoint protein MAD2A



Danio rerio (zebrafish) mad2l1 NP_001017739.1
Kluyveromyces lactis KLLA0D09438g XP_453482.1
Macaca mulatta (rhesus monkey) MAD2L1 XP_001097054.2
Pan troglodytes (chimpanzee) MAD2L1 XP_003310508.1
Saccharomyces cerevisiae (baker's yeast) MAD2 NP_012504.3
Eremothecium gossypii AGOS_ACR043W NP_983446.1
Gallus gallus (chicken) MAD2L1 NP_001264678.1
Magnaporthe oryzae (rice blast fungus) MGG_08269 XP_003715816.1
Arabidopsis thaliana (thale cress) MAD2 NP_189227.1
Homo sapiens (human) MAD2L1 NP_002349.1
Bos taurus (cattle) MAD2L1 NP_001178442.1
Mus musculus (house mouse) Mad2l1 NP_062372.2
Drosophila melanogaster (fruit fly) mad2 NP_647991.1
Schizosaccharomyces pombe (fission yeast) mad2 NP_596370.1
Oryza sativa (rice) Os04g0486500 NP_001053138.1
Canis lupus familiaris (dog) LOC476070 XP_533278.2
Rattus norvegicus (Norway rat) Mad2l1 NP_001100064.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP000751 XP_311286.5
Caenorhabditis elegans (roundworm) mdf-2 NP_001023563.1
Neurospora crassa NCU05781 XP_960041.2
Xenopus tropicalis (tropical clawed frog) mad2l1 NP_001096528.1


Related articles in PubMed

Mad2 overexpression is associated with high cell proliferation and reduced disease-free survival in primary gastrointestinal diffuse large B-cell lymphoma.
Chen F, Liu S, Zhou Y, Shen H, Zuo X
Hematology (Amsterdam, Netherlands)21(7)399-403(2016 Aug)

Histone H3K4 methylation regulates deactivation of the spindle assembly checkpoint through direct binding of Mad2.
Schibler A, Koutelou E, Tomida J, Wilson-Pham M, Wang L, Lu Y, Cabrera AP, Chosed RJ, Li W, Li B, Shi X, Wood RD, Dent SY
Genes & development30(10)1187-97(2016 05)

Stable folding intermediates prevent fast interconversion between the closed and open states of Mad2 through its denatured state.
Zhao Y, Li L, Wu C, Jiang X, Ge B, Ren H, Huang F
Protein engineering, design & selection : PEDS29(1)23-9(2016 Jan)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

p31comet-Induced Cell Death Is Mediated by Binding and Inactivation of Mad2.
Shin HJ, Park ER, Yun SH, Kim SH, Jung WH, Woo SR, Joo HY, Jang SH, Chung HY, Hong SH, Cho MH, Park JJ, Yun M, Lee KH
PloS one10(11)e0141523(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thes authors show that p31(comet) binding to the TRIP13 N-terminal domain positions the disordered MAD2 N-terminus for engagement by the TRIP13 "pore loops", which then unfold MAD2 in the presence of ATP.
Title: The AAA+ ATPase TRIP13 remodels HORMA domains through N-terminal engagement and unfolding.

catalytic activation of the spindle assembly checkpoint depends on regulated protein-protein interactions that accelerate the spontaneous but rate-limiting conversion of MAD2 required for mitotic checkpoint complex assembly
Title: Basis of catalytic assembly of the mitotic checkpoint complex.

Overexpression of MAD2gamma may play a role in checkpoint disruption and is associated with resistance to cisplatin-based chemotherapy in testicular germ cell tumors.
Title: MAD2γ, a novel MAD2 isoform, reduces mitotic arrest and is associated with resistance in testicular germ cell tumors.

in addition to its role in checkpoint signaling, MAD2 ensures chromosome stability through the regulation of AURORA B.
Title: Regulation of AURORA B function by mitotic checkpoint protein MAD2.

interactions between Mad2 and H3K4 regulate resolution of the spindle assembly checkpoint by limiting closed Mad2 availability for Cdc20 inhibition.
Title: Histone H3K4 methylation regulates deactivation of the spindle assembly checkpoint through direct binding of Mad2.

The following MAD2L1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAD2L1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OHu24019 NM_002358.3
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Homo sapiens mitotic arrest deficient 2 like 1 (MAD2L1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu24019
Clone ID Related Accession (Same CDS sequence) NM_002358.3
Accession Version NM_002358.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 618bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-04
Organism Homo sapiens(Human)
Product mitotic spindle assembly checkpoint protein MAD2A
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA443733.1, AK313827.1, AK223433.1 and BC000356.2. On Jul 30, 2008 this sequence version replaced NM_002358.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.100870.1, SRR1163657.407321.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
ATGGCGCTGC AGCTCTCCCG GGAGCAGGGA ATCACCCTGC GCGGGAGCGC CGAAATCGTG 
GCCGAGTTCT TCTCATTCGG CATCAACAGC ATTTTATATC AGCGTGGCAT ATATCCATCT
GAAACCTTTA CTCGAGTGCA GAAATACGGA CTCACCTTGC TTGTAACTAC TGATCTTGAG
CTCATAAAAT ACCTAAATAA TGTGGTGGAA CAACTGAAAG ATTGGTTATA CAAGTGTTCA
GTTCAGAAAC TGGTTGTAGT TATCTCAAAT ATTGAAAGTG GTGAGGTCCT GGAAAGATGG
CAGTTTGATA TTGAGTGTGA CAAGACTGCA AAAGATGACA GTGCACCCAG AGAAAAGTCT
CAGAAAGCTA TCCAGGATGA AATCCGTTCA GTGATCAGAC AGATCACAGC TACGGTGACA
TTTCTGCCAC TGTTGGAAGT TTCTTGTTCA TTTGATCTGC TGATTTATAC AGACAAAGAT
TTGGTTGTAC CTGAAAAATG GGAAGAGTCG GGACCACAGT TTATTACCAA TTCTGAGGAA
GTCCGCCTTC GTTCATTTAC TACTACAATC CACAAAGTAA ATAGCATGGT GGCCTACAAA
ATTCCTGTCA ATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002349.1
CDS125..742
Misc Feature(1)128..130
Misc Feature(2)140..142
Misc Feature(3)161..697
Misc Feature(4)512..514
Misc Feature(5)632..634
Misc Feature(6)632..634
Misc Feature(7)656..658
Misc Feature(8)656..658
Misc Feature(9)677..679
Misc Feature(10)707..739
Misc Feature(11)707..709
Misc Feature(12)707..709
Exon (1)1..197
Exon (2)198..344
Exon (3)345..465
Exon (4)466..569
Exon (5)570..1438
Translation

Target ORF information:

RefSeq Version NM_002358.3
Organism Homo sapiens(Human)
Definition Homo sapiens mitotic arrest deficient 2 like 1 (MAD2L1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002358.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
ATGGCGCTGC AGCTCTCCCG GGAGCAGGGA ATCACCCTGC GCGGGAGCGC CGAAATCGTG 
GCCGAGTTCT TCTCATTCGG CATCAACAGC ATTTTATATC AGCGTGGCAT ATATCCATCT
GAAACCTTTA CTCGAGTGCA GAAATACGGA CTCACCTTGC TTGTAACTAC TGATCTTGAG
CTCATAAAAT ACCTAAATAA TGTGGTGGAA CAACTGAAAG ATTGGTTATA CAAGTGTTCA
GTTCAGAAAC TGGTTGTAGT TATCTCAAAT ATTGAAAGTG GTGAGGTCCT GGAAAGATGG
CAGTTTGATA TTGAGTGTGA CAAGACTGCA AAAGATGACA GTGCACCCAG AGAAAAGTCT
CAGAAAGCTA TCCAGGATGA AATCCGTTCA GTGATCAGAC AGATCACAGC TACGGTGACA
TTTCTGCCAC TGTTGGAAGT TTCTTGTTCA TTTGATCTGC TGATTTATAC AGACAAAGAT
TTGGTTGTAC CTGAAAAATG GGAAGAGTCG GGACCACAGT TTATTACCAA TTCTGAGGAA
GTCCGCCTTC GTTCATTTAC TACTACAATC CACAAAGTAA ATAGCATGGT GGCCTACAAA
ATTCCTGTCA ATGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Mad2 overexpression is associated with high cell proliferation and reduced disease-free survival in primary gastrointestinal diffuse large B-cell lymphoma.
Hematology (Amsterdam, Netherlands)21(7)399-403(2016 Aug)
Chen F,Liu S,Zhou Y,Shen H,Zuo X


book

Histone H3K4 methylation regulates deactivation of the spindle assembly checkpoint through direct binding of Mad2.
Genes & development30(10)1187-97(2016 05)
Schibler A,Koutelou E,Tomida J,Wilson-Pham M,Wang L,Lu Y,Cabrera AP,Chosed RJ,Li W,Li B,Shi X,Wood RD,Dent SY


book

Stable folding intermediates prevent fast interconversion between the closed and open states of Mad2 through its denatured state.
Protein engineering, design & selection : PEDS29(1)23-9(2016 Jan)
Zhao Y,Li L,Wu C,Jiang X,Ge B,Ren H,Huang F


book

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M


book

p31comet-Induced Cell Death Is Mediated by Binding and Inactivation of Mad2.
PloS one10(11)e0141523(2015)
Shin HJ,Park ER,Yun SH,Kim SH,Jung WH,Woo SR,Joo HY,Jang SH,Chung HY,Hong SH,Cho MH,Park JJ,Yun M,Lee KH


 
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