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SMAD4 cDNA ORF clone, Homo sapiens (human)

Gene Symbol SMAD4
Entrez Gene ID 4089
Full Name SMAD family member 4
Synonyms DPC4, JIP, MADH4, MYHRS
General protein information
Preferred Names
mothers against decapentaplegic homolog 4
Names
mothers against decapentaplegic homolog 4
MAD homolog 4
SMAD, mothers against DPP homolog 4
deleted in pancreatic carcinoma locus 4
deletion target in pancreatic carcinoma 4
mothers against decapentaplegic, Drosophila, homolog of, 4
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

18

18q21.1

Summary This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]. lac of sum
Disorder MIM:

600993

Disorder Html: Pancreatic cancer (3); Polyposis, juvenile intestinal, 174900 (3);

mRNA and Protein(s)

mRNA Protein Name
NM_005359 NP_005350 mothers against decapentaplegic homolog 4



Homo sapiens (human) SMAD4 NP_005350.1
Pan troglodytes (chimpanzee) SMAD4 XP_001155601.1
Macaca mulatta (Rhesus monkey) SMAD4 XP_001096658.1
Canis lupus familiaris (dog) SMAD4 XP_005615451.1
Bos taurus (cattle) SMAD4 NP_001069677.1
Mus musculus (house mouse) Smad4 NP_032566.2
Rattus norvegicus (Norway rat) Smad4 NP_062148.1
Danio rerio (zebrafish) zmp:0000000768 NP_001116172.1
Drosophila melanogaster (fruit fly) Med NP_524610.1
Xenopus (Silurana) tropicalis (western clawed frog) smad4.1 XP_002934485.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SMAD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMAD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu26813
NM_005359 Homo sapiens SMAD family member 4 (SMAD4), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu26813
Clone ID Related Accession (Same CDS sequence) NM_005359
Accession Version NM_005359.5 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1659bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product mothers against decapentaplegic homolog 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU120224.1, BC002379.2, AC091551.14 and BM701399.1. This sequence is a reference standard in the RefSeqGene project. On Aug 8, 2008 this sequence version replaced gi:164565433. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC002379.2, U44378.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGACAATA TGTCTATTAC GAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTG 
CATAGTTTGA TGTGCCATAG ACAAGGTGGA GAGAGTGAAA CATTTGCAAA AAGAGCAATT
GAAAGTTTGG TAAAGAAGCT GAAGGAGAAA AAAGATGAAT TGGATTCTTT AATAACAGCT
ATAACTACAA ATGGAGCTCA TCCTAGTAAA TGTGTTACCA TACAGAGAAC ATTGGATGGG
AGGCTTCAGG TGGCTGGTCG GAAAGGATTT CCTCATGTGA TCTATGCCCG TCTCTGGAGG
TGGCCTGATC TTCACAAAAA TGAACTAAAA CATGTTAAAT ATTGTCAGTA TGCGTTTGAC
TTAAAATGTG ATAGTGTCTG TGTGAATCCA TATCACTACG AACGAGTTGT ATCACCTGGA
ATTGATCTCT CAGGATTAAC ACTGCAGAGT AATGCTCCAT CAAGTATGAT GGTGAAGGAT
GAATATGTGC ATGACTTTGA GGGACAGCCA TCGTTGTCCA CTGAAGGACA TTCAATTCAA
ACCATCCAGC ATCCACCAAG TAATCGTGCA TCGACAGAGA CATACAGCAC CCCAGCTCTG
TTAGCCCCAT CTGAGTCTAA TGCTACCAGC ACTGCCAACT TTCCCAACAT TCCTGTGGCT
TCCACAAGTC AGCCTGCCAG TATACTGGGG GGCAGCCATA GTGAAGGACT GTTGCAGATA
GCATCAGGGC CTCAGCCAGG ACAGCAGCAG AATGGATTTA CTGGTCAGCC AGCTACTTAC
CATCATAACA GCACTACCAC CTGGACTGGA AGTAGGACTG CACCATACAC ACCTAATTTG
CCTCACCACC AAAACGGCCA TCTTCAGCAC CACCCGCCTA TGCCGCCCCA TCCCGGACAT
TACTGGCCTG TTCACAATGA GCTTGCATTC CAGCCTCCCA TTTCCAATCA TCCTGCTCCT
GAGTATTGGT GTTCCATTGC TTACTTTGAA ATGGATGTTC AGGTAGGAGA GACATTTAAG
GTTCCTTCAA GCTGCCCTAT TGTTACTGTT GATGGATACG TGGACCCTTC TGGAGGAGAT
CGCTTTTGTT TGGGTCAACT CTCCAATGTC CACAGGACAG AAGCCATTGA GAGAGCAAGG
TTGCACATAG GCAAAGGTGT GCAGTTGGAA TGTAAAGGTG AAGGTGATGT TTGGGTCAGG
TGCCTTAGTG ACCACGCGGT CTTTGTACAG AGTTACTACT TAGACAGAGA AGCTGGGCGT
GCACCTGGAG ATGCTGTTCA TAAGATCTAC CCAAGTGCAT ATATAAAGGT CTTTGATTTG
CGTCAGTGTC ATCGACAGAT GCAGCAGCAG GCGGCTACTG CACAAGCTGC AGCAGCTGCC
CAGGCAGCAG CCGTGGCAGG AAACATCCCT GGCCCAGGAT CAGTAGGTGG AATAGCTCCA
GCTATCAGTC TGTCAGCTGC TGCTGGAATT GGTGTTGATG ACCTTCGTCG CTTATGCATA
CTCAGGATGA GTTTTGTGAA AGGCTGGGGA CCGGATTACC CAAGACAGAG CATCAAAGAA
ACACCTTGCT GGATTGAAAT TCACTTACAC CGGGCCCTCC AGCTCCTAGA CGAAGTACTT
CATACCATGC CGATTGCAGA CCCACAACCT TTAGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005350.1
CDS539..2197
Misc Feature(1)518..520(+)
Misc Feature(2)578..952(+)
Misc Feature(3)647..649(+)
Misc Feature(4)650..859(+)
Misc Feature(5)749..934(+)
Misc Feature(6)875..877(+)
Misc Feature(7)875..877(+)
Misc Feature(8)1013..1015(+)
Misc Feature(9)1013..1015(+)
Misc Feature(10)1361..1498(+)
Misc Feature(11)1367..1369(+)
Misc Feature(12)1496..2161(+)
Misc Feature(13)1523..2161(+)
Misc Feature(14)1820..1822(+)
Misc Feature(15)2057..2059(+)
Misc Feature(16)2081..2083(+)
Exon (1)1..411
Gene:SMAD4
Gene Synonym:
Exon (2)412..787
Gene:SMAD4
Gene Synonym:
Exon (3)788..962
Gene:SMAD4
Gene Synonym:
Exon (4)963..992
Gene:SMAD4
Gene Synonym:
Exon (5)993..1205
Gene:SMAD4
Gene Synonym:
Exon (6)1206..1325
Gene:SMAD4
Gene Synonym:
Exon (7)1326..1442
Gene:SMAD4
Gene Synonym:
Exon (8)1443..1493
Gene:SMAD4
Gene Synonym:
Exon (9)1494..1677
Gene:SMAD4
Gene Synonym:
Exon (10)1678..1846
Gene:SMAD4
Gene Synonym:
Exon (11)1847..1985
Gene:SMAD4
Gene Synonym:
Exon (12)1986..8772
Gene:SMAD4
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_005359
Organism Homo sapiens (human)
Definition Homo sapiens SMAD family member 4 (SMAD4), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005359

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGGACAATA TGTCTATTAC GAATACACCA ACAAGTAATG ATGCCTGTCT GAGCATTGTG 
CATAGTTTGA TGTGCCATAG ACAAGGTGGA GAGAGTGAAA CATTTGCAAA AAGAGCAATT
GAAAGTTTGG TAAAGAAGCT GAAGGAGAAA AAAGATGAAT TGGATTCTTT AATAACAGCT
ATAACTACAA ATGGAGCTCA TCCTAGTAAA TGTGTTACCA TACAGAGAAC ATTGGATGGG
AGGCTTCAGG TGGCTGGTCG GAAAGGATTT CCTCATGTGA TCTATGCCCG TCTCTGGAGG
TGGCCTGATC TTCACAAAAA TGAACTAAAA CATGTTAAAT ATTGTCAGTA TGCGTTTGAC
TTAAAATGTG ATAGTGTCTG TGTGAATCCA TATCACTACG AACGAGTTGT ATCACCTGGA
ATTGATCTCT CAGGATTAAC ACTGCAGAGT AATGCTCCAT CAAGTATGAT GGTGAAGGAT
GAATATGTGC ATGACTTTGA GGGACAGCCA TCGTTGTCCA CTGAAGGACA TTCAATTCAA
ACCATCCAGC ATCCACCAAG TAATCGTGCA TCGACAGAGA CATACAGCAC CCCAGCTCTG
TTAGCCCCAT CTGAGTCTAA TGCTACCAGC ACTGCCAACT TTCCCAACAT TCCTGTGGCT
TCCACAAGTC AGCCTGCCAG TATACTGGGG GGCAGCCATA GTGAAGGACT GTTGCAGATA
GCATCAGGGC CTCAGCCAGG ACAGCAGCAG AATGGATTTA CTGGTCAGCC AGCTACTTAC
CATCATAACA GCACTACCAC CTGGACTGGA AGTAGGACTG CACCATACAC ACCTAATTTG
CCTCACCACC AAAACGGCCA TCTTCAGCAC CACCCGCCTA TGCCGCCCCA TCCCGGACAT
TACTGGCCTG TTCACAATGA GCTTGCATTC CAGCCTCCCA TTTCCAATCA TCCTGCTCCT
GAGTATTGGT GTTCCATTGC TTACTTTGAA ATGGATGTTC AGGTAGGAGA GACATTTAAG
GTTCCTTCAA GCTGCCCTAT TGTTACTGTT GATGGATACG TGGACCCTTC TGGAGGAGAT
CGCTTTTGTT TGGGTCAACT CTCCAATGTC CACAGGACAG AAGCCATTGA GAGAGCAAGG
TTGCACATAG GCAAAGGTGT GCAGTTGGAA TGTAAAGGTG AAGGTGATGT TTGGGTCAGG
TGCCTTAGTG ACCACGCGGT CTTTGTACAG AGTTACTACT TAGACAGAGA AGCTGGGCGT
GCACCTGGAG ATGCTGTTCA TAAGATCTAC CCAAGTGCAT ATATAAAGGT CTTTGATTTG
CGTCAGTGTC ATCGACAGAT GCAGCAGCAG GCGGCTACTG CACAAGCTGC AGCAGCTGCC
CAGGCAGCAG CCGTGGCAGG AAACATCCCT GGCCCAGGAT CAGTAGGTGG AATAGCTCCA
GCTATCAGTC TGTCAGCTGC TGCTGGAATT GGTGTTGATG ACCTTCGTCG CTTATGCATA
CTCAGGATGA GTTTTGTGAA AGGCTGGGGA CCGGATTACC CAAGACAGAG CATCAAAGAA
ACACCTTGCT GGATTGAAAT TCACTTACAC CGGGCCCTCC AGCTCCTAGA CGAAGTACTT
CATACCATGC CGATTGCAGA CCCACAACCT TTAGACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Zinc finger protein 451 is a novel Smad corepressor in transforming growth factor-beta signaling
J. Biol. Chem. 289 (4), 2072-2083 (2014)
Feng Y, Wu H, Xu Y, Zhang Z, Liu T, Lin X and Feng XH.


book

A study of Smad4 and Smad7 expression in surgically resected samples of gastric adenocarcinoma and their correlation with clinicopathological parameters and patient survival
J BUON 19 (1), 221-227 (2014)
Zizi-Sermpetzoglou A, Myoteri D, Arkoumani E, Voultsos M and Marinis A.


book

Targeted deletion of Smad4 shows it is required for transforming growth factor beta and activin signaling in colorectal cancer cells
Proc. Natl. Acad. Sci. U.S.A. 95 (5), 2412-2416 (1998)
Zhou S, Buckhaults P, Zawel L, Bunz F, Riggins G, Dai JL, Kern SE, Kinzler KW and Vogelstein B.


book

Human Smad3 and Smad4 are sequence-specific transcription activators
Mol. Cell 1 (4), 611-617 (1998)
Zawel L, Dai JL, Buckhaults P, Zhou S, Kinzler KW, Vogelstein B and Kern SE.


book

Nomenclature: vertebrate mediators of TGFbeta family signals
Cell 87 (2), 173 (1996)
Derynck,R., Gelbart,W.M., Harland,R.M., Heldin,C.H., Kern,S.E., Massague,J., Melton,D.A., Mlodzik,M., Padgett,R.W., Roberts,A.B., Smith,J., Thomsen,G.H., Vogelstein,B. and Wang,X.F.


book

Receptor-associated Mad homologues synergize as effectors of the TGF-beta response
Nature 383 (6596), 168-172 (1996)
Zhang Y, Feng X, We R and Derynck R.


book

DPC4, a candidate tumor suppressor gene at human chromosome 18q21.1
Science 271 (5247), 350-353 (1996)
Hahn SA, Schutte M, Hoque AT, Moskaluk CA, da Costa LT, Rozenblum E, Weinstein CL, Fischer A, Yeo CJ, Hruban RH and Kern SE.


book

E2F-4, a new member of the E2F transcription factor family, interacts with p107
Genes Dev. 8 (22), 2665-2679 (1994)
Ginsberg D, Vairo G, Chittenden T, Xiao ZX, Xu G, Wydner KL, DeCaprio JA, Lawrence JB and Livingston DM.


book

Juvenile Polyposis Syndrome
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
Larsen Haidle,J. and Howe,J.R.


book

Hereditary Hemorrhagic Telangiectasia
(in) Pagon RA, Adam MP, Ardinger HH, Bird TD, Dolan CR, Fong CT, Smith RJH and Stephens K (Eds.); GENEREVIEWS(R); (1993)
McDonald,J. and Pyeritz,R.E.


 
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