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MAGEA2 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MAGEA2
Entrez Gene ID 4101
Full Name MAGE family member A2
Synonyms CT1.2,MAGE2,MAGEA2A
General protein information
Preferred Names

MAGE family member A2

Names

melanoma-associated antigen 2
MAGE-2 antigen
cancer/testis antigen 1.2
cancer/testis antigen family 1, member 2
melanoma antigen 2
melanoma antigen family A, 2
melanoma antigen family A2

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

X

Xq28

Summary This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This gene has two identical copies at different loci. Alternatively spliced transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

300173


mRNA and Protein(s)

mRNA Protein Name
NM_001282505.1 NP_001269434.1 melanoma-associated antigen 2
NM_001282504.1 NP_001269433.1 melanoma-associated antigen 2
XM_017029519.1 XP_016885008.1 melanoma-associated antigen 2 isoform X1
XM_011531159.1 XP_011529461.1 melanoma-associated antigen 2 isoform X1
NM_175743.2 NP_786885.1 melanoma-associated antigen 2
NM_005361.3 NP_005352.1 melanoma-associated antigen 2
NM_001282502.1 NP_001269431.1 melanoma-associated antigen 2
NM_175742.2 NP_786884.1 melanoma-associated antigen 2
NM_001282501.1 NP_001269430.1 melanoma-associated antigen 2



Pan troglodytes (chimpanzee) MAGEA2 XP_003318187.1
Macaca mulatta (rhesus monkey) MAGEA2 XP_001095272.1
Homo sapiens (human) MAGEA2 NP_001269434.1
Homo sapiens (human) MAGEA2B NP_705692.1


Related articles in PubMed

Identification of MAGEA antigens as causal players in the development of tamoxifen-resistant breast cancer.
Wong PP, Yeoh CC, Ahmad AS, Chelala C, Gillett C, Speirs V, Jones JL, Hurst HC
Oncogene33(37)4579-88(2014 Sep)

The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM.
Zeng L, Kang C, Di C, Fee BE, Rivas M, Lin J, Adamson DC
International journal of oncology44(4)1243-51(2014 Apr)

MageA2 restrains cellular senescence by targeting the function of PMLIV/p53 axis at the PML-NBs.
Peche LY, Scolz M, Ladelfa MF, Monte M, Schneider C
Cell death and differentiation19(6)926-36(2012 Jun)

MAGE A1-A6 RT-PCR and MAGE A3 and p16 methylation analysis in induced sputum from patients with lung cancer and non-malignant lung diseases.
Shin KC, Lee KH, Lee CH, Shin IH, Suh HS, Jeon CH
Oncology reports27(4)911-6(2012 Apr)

The role of MAGEA2 in head and neck cancer.
Glazer CA, Smith IM, Bhan S, Sun W, Chang SS, Pattani KM, Westra W, Khan Z, Califano JA
Archives of otolaryngology--head & neck surgery137(3)286-93(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Immunohistochemistry using D2-40 monoclonal antibody (MAGE2) and anti-MITF1 increased detection of lymphovascular invasion in primary cutaneous melanoma.
Title: Prognostic Implication of Lymphovascular Invasion Detected by Double Immunostaining for D2-40 and MITF1 in Primary Cutaneous Melanoma.

We find that MAGE-A2 interacts with MDM2 via the N-terminal p53-binding pocket and the RING finger domain of MDM2 that is required for homo/hetero-dimerization and for E2 ligase interaction.
Title: MAGE-A Cancer/Testis Antigens Inhibit MDM2 Ubiquitylation Function and Promote Increased Levels of MDM4.

MAGEA2 has a critical role in the development of tamoxifen-resistant breast cancer
Title: Identification of MAGEA antigens as causal players in the development of tamoxifen-resistant breast cancer.

Consistent with increased cell death, the induced loss of MAGEA2 expression correlated with increased caspase 3/7 activity, BCL2/BAX ratio and TUNEL signal.
Title: The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM.

Report MAGEA1-A6 expression in sputum suggests presence of lung cancer cells or precancerous cells.
Title: MAGE A1-A6 RT-PCR and MAGE A3 and p16 methylation analysis in induced sputum from patients with lung cancer and non-malignant lung diseases.

The following MAGEA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGEA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu00411 NM_175743.2
Latest version!
Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu00411 NM_001282504.1
Latest version!
Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu00411 NM_005361.3
Latest version!
Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu00411 NM_175742.2
Latest version!
Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu00411 NM_001282502.1
Latest version!
Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu00411 NM_001282501.1
Latest version!
Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu00411 NM_001282505.1
Latest version!
Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu00411 XM_017029519.1
Latest version!
Homo sapiens MAGE family member A2 (MAGEA2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu00411 XM_011531159.1
Latest version!
Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu00411
Clone ID Related Accession (Same CDS sequence) NM_001282505.1 , NM_001282504.1 , XM_017029519.1 , XM_011531159.1 , NM_175743.2 , NM_005361.3 , NM_001282502.1 , NM_175742.2 , NM_001282501.1
Accession Version NM_001282505.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-15
Organism Homo sapiens(Human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290443.1, AU100045.1, BC108720.1 and CA429655.1. On or before Sep 12, 2013 this sequence version replaced XM_005274675.1, XM_005277849.1. Transcript Variant: This variant (7) lacks four internal exons in the 5' UTR region, compared to variant 3. Variants 1 through 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC109188.1, AU100045.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269434.1
CDS163..1107
Misc Feature(1)52..54
Misc Feature(2)175..444
Misc Feature(3)352..354
Misc Feature(4)508..1002
Exon (1)1..97
Exon (2)98..1663
Translation

Target ORF information:

RefSeq Version NM_001282505.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282505.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Clone ID Related Accession (Same CDS sequence) NM_001282505.1 , NM_001282504.1 , XM_017029519.1 , XM_011531159.1 , NM_175743.2 , NM_005361.3 , NM_001282502.1 , NM_175742.2 , NM_001282501.1
Accession Version NM_001282504.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-15
Organism Homo sapiens(Human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290443.1, DR158683.1, BC108720.1 and CA429655.1. On or before Sep 12, 2013 this sequence version replaced XM_005274674.1, XM_005277848.1. Transcript Variant: This variant (6) lacks three internal exons in the 5' UTR region, compared to variant 3. Variants 1 through 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BF791379.1, DR158683.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269433.1
CDS229..1173
Misc Feature(1)52..54
Misc Feature(2)241..510
Misc Feature(3)418..420
Misc Feature(4)574..1068
Exon (1)1..97
Exon (2)98..163
Exon (3)164..1729
Translation

Target ORF information:

RefSeq Version NM_001282504.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282504.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Clone ID Related Accession (Same CDS sequence) NM_001282505.1 , NM_001282504.1 , XM_017029519.1 , XM_011531159.1 , NM_175743.2 , NM_005361.3 , NM_001282502.1 , NM_175742.2 , NM_001282501.1
Accession Version NM_175743.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-15
Organism Homo sapiens(Human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290443.1, BX282627.1, BC108720.1 and CA429655.1. On Sep 12, 2013 this sequence version replaced NM_175743.1. Transcript Variant: This variant (3) is the longest transcript. Variants 1 through 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG575302.1, BI859928.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_786885.1
CDS526..1470
Misc Feature(1)337..339
Misc Feature(2)538..807
Misc Feature(3)715..717
Misc Feature(4)871..1365
Exon (1)1..97
Exon (2)98..176
Exon (3)177..296
Exon (4)297..394
Exon (5)395..460
Exon (6)461..2026
Translation

Target ORF information:

RefSeq Version NM_175743.2
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_175743.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Clone ID Related Accession (Same CDS sequence) NM_001282505.1 , NM_001282504.1 , XM_017029519.1 , XM_011531159.1 , NM_175743.2 , NM_005361.3 , NM_001282502.1 , NM_175742.2 , NM_001282501.1
Accession Version NM_005361.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-15
Organism Homo sapiens(Human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC244102.3. On Sep 12, 2013 this sequence version replaced NM_005361.2. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 3. Variants 1 through 7 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: CA453951.1, BF793468.1 [ECO:0000331] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005352.1
CDS524..1468
Misc Feature(1)389..391
Misc Feature(2)536..805
Misc Feature(3)713..715
Misc Feature(4)869..1363
Exon (1)1..272
Exon (2)273..392
Exon (3)393..458
Exon (4)459..2024
Translation

Target ORF information:

RefSeq Version NM_005361.3
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005361.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Clone ID Related Accession (Same CDS sequence) NM_001282505.1 , NM_001282504.1 , XM_017029519.1 , XM_011531159.1 , NM_175743.2 , NM_005361.3 , NM_001282502.1 , NM_175742.2 , NM_001282501.1
Accession Version NM_001282502.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-15
Organism Homo sapiens(Human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290443.1, BC108720.1 and CA429655.1. On or before Sep 12, 2013 this sequence version replaced XM_005274673.1, XM_005277847.1. Transcript Variant: This variant (5) lacks two internal exons in the 5' UTR region, compared to variant 3. Variants 1 through 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC108720.1, DR157723.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269431.1
CDS349..1293
Misc Feature(1)214..216
Misc Feature(2)361..630
Misc Feature(3)538..540
Misc Feature(4)694..1188
Exon (1)1..97
Exon (2)98..217
Exon (3)218..283
Exon (4)284..1849
Translation

Target ORF information:

RefSeq Version NM_001282502.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282502.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Clone ID Related Accession (Same CDS sequence) NM_001282505.1 , NM_001282504.1 , XM_017029519.1 , XM_011531159.1 , NM_175743.2 , NM_005361.3 , NM_001282502.1 , NM_175742.2 , NM_001282501.1
Accession Version NM_175742.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-15
Organism Homo sapiens(Human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290443.1, BM781931.1, BC108720.1 and CA429655.1. On Sep 12, 2013 this sequence version replaced NM_175742.1. Transcript Variant: This variant (2) has an additional exon in the 5' region and lacks an alternate exon in a downstream region, compared to variant 3. Variants 1 through 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM781931.1, BM846411.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_786884.1
CDS510..1454
Misc Feature(1)375..377
Misc Feature(2)522..791
Misc Feature(3)699..701
Misc Feature(4)855..1349
Exon (1)1..97
Exon (2)98..179
Exon (3)180..258
Exon (4)259..378
Exon (5)379..444
Exon (6)445..2010
Translation

Target ORF information:

RefSeq Version NM_175742.2
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_175742.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Clone ID Related Accession (Same CDS sequence) NM_001282505.1 , NM_001282504.1 , XM_017029519.1 , XM_011531159.1 , NM_175743.2 , NM_005361.3 , NM_001282502.1 , NM_175742.2 , NM_001282501.1
Accession Version NM_001282501.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-15
Organism Homo sapiens(Human)
Product melanoma-associated antigen 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290443.1, BC063681.1 and CA429655.1. On or before Sep 12, 2013 this sequence version replaced XM_005274672.1, XM_005277846.1. Transcript Variant: This variant (4) lacks an internal exon in the 5' UTR region, compared to variant 3. Variants 1 through 7 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290443.1, CA489674.1 [ECO:0000332] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269430.1
CDS447..1391
Misc Feature(1)258..260
Misc Feature(2)459..728
Misc Feature(3)636..638
Misc Feature(4)792..1286
Exon (1)1..97
Exon (2)98..217
Exon (3)218..315
Exon (4)316..381
Exon (5)382..1947
Translation

Target ORF information:

RefSeq Version NM_001282501.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A2 (MAGEA2), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282501.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Clone ID Related Accession (Same CDS sequence) NM_001282505.1 , NM_001282504.1 , XM_017029519.1 , XM_011531159.1 , NM_175743.2 , NM_005361.3 , NM_001282502.1 , NM_175742.2 , NM_001282501.1
Accession Version XM_017029519.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen 2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016885008.1
CDS486..1430
Misc Feature(1)498..767
Misc Feature(2)831..1325
Translation

Target ORF information:

RefSeq Version XM_017029519.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A2 (MAGEA2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017029519.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00411
Clone ID Related Accession (Same CDS sequence) NM_001282505.1 , NM_001282504.1 , XM_017029519.1 , XM_011531159.1 , NM_175743.2 , NM_005361.3 , NM_001282502.1 , NM_175742.2 , NM_001282501.1
Accession Version XM_011531159.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product melanoma-associated antigen 2 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529461.1
Translation

Target ORF information:

RefSeq Version XM_011531159.1
Organism Homo sapiens(Human)
Definition Homo sapiens melanoma antigen family A2 (MAGEA2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531159.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGCA GACCGCTTCT
TCCTCTTCTA CTCTAGTGGA AGTTACCCTG GGGGAGGTGC CTGCTGCCGA CTCACCGAGT
CCTCCCCACA GTCCTCAGGG AGCCTCCAGC TTCTCGACTA CCATCAACTA CACTCTTTGG
AGACAATCCG ATGAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGAAT GTTTCCCGAC
CTGGAGTCCG AGTTCCAAGC AGCAATCAGT AGGAAGATGG TTGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGA GAGTGTCCTC
AGAAATTGCC AGGACTTCTT TCCCGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CCCATCAGCC ACTTGTACAT CCTTGTCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGGTCATGCC CAAGACAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA ATAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTAT GTTGGAGGTG TTTGAGGGGA GGGAGGACAG TGTCTTCGCA
CATCCCAGGA AGCTGCTCAT GCAAGATCTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTGCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCATTGAA
ACCAGCTATG TGAAAGTCCT GCACCATACA CTAAAGATCG GTGGAGAACC TCACATTTCC
TACCCACCCC TGCATGAACG GGCTTTGAGA GAGGGAGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Identification of MAGEA antigens as causal players in the development of tamoxifen-resistant breast cancer.
Oncogene33(37)4579-88(2014 Sep)
Wong PP,Yeoh CC,Ahmad AS,Chelala C,Gillett C,Speirs V,Jones JL,Hurst HC


book

The adherens junction-associated protein 1 is a negative transcriptional regulator of MAGEA2, which potentiates temozolomide-induced apoptosis in GBM.
International journal of oncology44(4)1243-51(2014 Apr)
Zeng L,Kang C,Di C,Fee BE,Rivas M,Lin J,Adamson DC


book

MageA2 restrains cellular senescence by targeting the function of PMLIV/p53 axis at the PML-NBs.
Cell death and differentiation19(6)926-36(2012 Jun)
Peche LY,Scolz M,Ladelfa MF,Monte M,Schneider C


book

MAGE A1-A6 RT-PCR and MAGE A3 and p16 methylation analysis in induced sputum from patients with lung cancer and non-malignant lung diseases.
Oncology reports27(4)911-6(2012 Apr)
Shin KC,Lee KH,Lee CH,Shin IH,Suh HS,Jeon CH


book

The role of MAGEA2 in head and neck cancer.
Archives of otolaryngology--head & neck surgery137(3)286-93(2011 Mar)
Glazer CA,Smith IM,Bhan S,Sun W,Chang SS,Pattani KM,Westra W,Khan Z,Califano JA


 
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