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MAGEA3 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MAGEA3
Entrez Gene ID 4102
Full Name MAGE family member A3
Synonyms CT1.3,HIP8,HYPD,MAGE3,MAGEA6
General protein information
Preferred Names

MAGE family member A3

Names

melanoma-associated antigen 3
MAGE-3 antigen
antigen MZ2-D
cancer/testis antigen 1.3
cancer/testis antigen family 1, member 3
melanoma antigen family A, 3
melanoma antigen family A3

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

X

Xq28

Summary This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008].
Disorder MIM:

300174


mRNA and Protein(s)

mRNA Protein Name
XM_011531160.1 XP_011529462.1 melanoma-associated antigen 3 isoform X1
XM_005274676.3 XP_005274733.1 melanoma-associated antigen 3 isoform X1
NM_005362.3 NP_005353.1 melanoma-associated antigen 3
XM_011531161.1 XP_011529463.1 melanoma-associated antigen 3 isoform X1
XM_005274676.2 XP_005274733.1 melanoma-associated antigen 3 isoform X1
XM_011531160.2 XP_011529462.1 melanoma-associated antigen 3 isoform X1
XM_006724818.2 XP_006724881.1 melanoma-associated antigen 3 isoform X1
XM_011531161.2 XP_011529463.1 melanoma-associated antigen 3 isoform X1



Macaca mulatta (rhesus monkey) LOC711544 XP_001100355.2
Homo sapiens (human) MAGEA6 NP_787064.1
Homo sapiens (human) MAGEA3 NP_005353.1
Macaca mulatta (rhesus monkey) MAGEA3 XP_001094934.2


Related articles in PubMed

Degradation of AMPK by a cancer-specific ubiquitin ligase.
Pineda CT, Ramanathan S, Fon Tacer K, Weon JL, Potts MB, Ou YH, White MA, Potts PR
Cell160(4)715-28(2015 Feb)

MAGE proteins regulate KRAB zinc finger transcription factors and KAP1 E3 ligase activity.
Xiao TZ, Suh Y, Longley BJ
Archives of biochemistry and biophysics563136-44(2014 Dec)

Clinical significance of immunohistochemical expression of cancer/testis tumor-associated antigens (MAGE-A1, MAGE-A3/4, NY-ESO-1) in patients with non-small cell lung cancer.
Grah JJ, Katalinic D, Juretic A, Santek F, Samarzija M
Tumori100(1)60-8(2014 Jan-Feb)

Expression and clinical significance of cancer-testis genes in clear cell renal cell carcinoma.
Yin B, Zeng Y, Wang X, Liu G, Zhang M, Song Y
International journal of clinical and experimental pathology7(7)4112-9(2014)

MAGE-A3 is highly expressed in a cancer stem cell-like side population of bladder cancer cells.
Yin B, Zeng Y, Liu G, Wang X, Wang P, Song Y
International journal of clinical and experimental pathology7(6)2934-41(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data demonstrate that MAGE-A1-, MAGE-A3-, and NY-ESO-1-specific T cells with antigen-specific cytotoxicity can be cultured from healthy donors and patient-derived cells making adoptive immunotherapy with these cytotoxic T lymphocyte feasible.
Title: Expansion of cancer germline antigen-specific cytotoxic T lymphocytes for immunotherapy.

Data show that overall survival (OS) was significantly lower for patients expressing pan-MAGE or MAGE-A1/A3/A4 in both independent cohorts.
Title: MAGE expression in head and neck squamous cell carcinoma primary tumors, lymph node metastases and respective recurrences-implications for immunotherapy.

MAGEA3 may be demethylated in MPNST and plexiform type neurofibroma in NF-1 patients
Title: MAGEA3 methylation status is associated with prognosis of malignant peripheral nerve sheath tumor and with neurofibroma type in neurofibromatosis type 1.

Comparing the overall expression of CTAs, a decreased expression of all melanoma-associated antigens (MAGEs) post-treatment and a slightly increased expression of New York esophageal squamous cell carcinoma 1 (NY-ESO-1) was visible. The simultaneous cytoplasmic and nuclear expression of pan-MAGE or MAGE-A3/A4 correlated with reduced treatment-failure-free-survival (TFFS).
Title: Influence of Photodynamic Therapy on the Expression of Cancer/Testis Antigens in Squamous Cell Carcinoma of the Head and Neck.

MAGEA3 was overexpressed in colorectal cancer tissue compared with healthy colon. MAGEA3 expression positively correlated with colorectal cancer progression.
Title: Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic Implications.

The following MAGEA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGEA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25590 NM_005362.3
Latest version!
Homo sapiens MAGE family member A3 (MAGEA3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu25590 XM_006724818.2
Latest version!
Homo sapiens MAGE family member A3 (MAGEA3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu25590 XM_011531161.2
Latest version!
Homo sapiens MAGE family member A3 (MAGEA3), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu25590 XM_005274676.3
Latest version!
Homo sapiens MAGE family member A3 (MAGEA3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu25590 XM_011531160.2
Latest version!
Homo sapiens MAGE family member A3 (MAGEA3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
View Old Accession Versions >>
OHu25590 XM_005274676.2 Homo sapiens melanoma antigen family A3 (MAGEA3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu25590 XM_011531160.1 Homo sapiens melanoma antigen family A3 (MAGEA3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu25590 XM_011531161.1 Homo sapiens melanoma antigen family A3 (MAGEA3), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25590
Clone ID Related Accession (Same CDS sequence) XM_011531160.1 , XM_005274676.3 , NM_005362.3 , XM_011531161.1 , XM_005274676.2 , XM_011531160.2 , XM_006724818.2 , XM_011531161.2
Accession Version NM_005362.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-25
Organism Homo sapiens(Human)
Product melanoma-associated antigen 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005963.1. This sequence is a reference standard in the RefSeqGene project. On Mar 18, 2003 this sequence version replaced NM_005362.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005963.1, BC016803.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_005353.1
CDS210..1154
Misc Feature(1)96..98
Misc Feature(2)222..491
Misc Feature(3)555..1049
Exon (1)1..78
Exon (2)79..144
Exon (3)145..1724
Translation

Target ORF information:

RefSeq Version NM_005362.3
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A3 (MAGEA3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_005362.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25590
Clone ID Related Accession (Same CDS sequence) XM_011531160.1 , XM_005274676.3 , NM_005362.3 , XM_011531161.1 , XM_005274676.2 , XM_011531160.2 , XM_006724818.2 , XM_011531161.2
Accession Version XM_011531160.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product melanoma-associated antigen 3 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529462.1
Translation

Target ORF information:

RefSeq Version XM_011531160.1
Organism Homo sapiens(Human)
Definition Homo sapiens melanoma antigen family A3 (MAGEA3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531160.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25590
Clone ID Related Accession (Same CDS sequence) XM_011531160.1 , XM_005274676.3 , NM_005362.3 , XM_011531161.1 , XM_005274676.2 , XM_011531160.2 , XM_006724818.2 , XM_011531161.2
Accession Version XM_005274676.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005274676.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
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361
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481
541
601
661
721
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841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274733.1
CDS284..1228
Misc Feature(1)296..565
Misc Feature(2)629..1123
Translation

Target ORF information:

RefSeq Version XM_005274676.3
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A3 (MAGEA3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274676.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25590
Clone ID Related Accession (Same CDS sequence) XM_011531160.1 , XM_005274676.3 , NM_005362.3 , XM_011531161.1 , XM_005274676.2 , XM_011531160.2 , XM_006724818.2 , XM_011531161.2
Accession Version XM_011531161.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product melanoma-associated antigen 3 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529463.1
Translation

Target ORF information:

RefSeq Version XM_011531161.1
Organism Homo sapiens(Human)
Definition Homo sapiens melanoma antigen family A3 (MAGEA3), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531161.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25590
Clone ID Related Accession (Same CDS sequence) XM_011531160.1 , XM_005274676.3 , NM_005362.3 , XM_011531161.1 , XM_005274676.2 , XM_011531160.2 , XM_006724818.2 , XM_011531161.2
Accession Version XM_005274676.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product melanoma-associated antigen 3 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274733.1
Translation

Target ORF information:

RefSeq Version XM_005274676.2
Organism Homo sapiens(Human)
Definition Homo sapiens melanoma antigen family A3 (MAGEA3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274676.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25590
Clone ID Related Accession (Same CDS sequence) XM_011531160.1 , XM_005274676.3 , NM_005362.3 , XM_011531161.1 , XM_005274676.2 , XM_011531160.2 , XM_006724818.2 , XM_011531161.2
Accession Version XM_011531160.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531160.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529462.1
CDS190..1134
Misc Feature(1)202..471
Misc Feature(2)535..1029
Translation

Target ORF information:

RefSeq Version XM_011531160.2
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A3 (MAGEA3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531160.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25590
Clone ID Related Accession (Same CDS sequence) XM_011531160.1 , XM_005274676.3 , NM_005362.3 , XM_011531161.1 , XM_005274676.2 , XM_011531160.2 , XM_006724818.2 , XM_011531161.2
Accession Version XM_006724818.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006724818.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006724881.1
CDS366..1310
Misc Feature(1)378..647
Misc Feature(2)711..1205
Translation

Target ORF information:

RefSeq Version XM_006724818.2
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A3 (MAGEA3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006724818.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu25590
Clone ID Related Accession (Same CDS sequence) XM_011531160.1 , XM_005274676.3 , NM_005362.3 , XM_011531161.1 , XM_005274676.2 , XM_011531160.2 , XM_006724818.2 , XM_011531161.2
Accession Version XM_011531161.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 945bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531161.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529463.1
CDS615..1559
Misc Feature(1)627..896
Misc Feature(2)960..1454
Translation

Target ORF information:

RefSeq Version XM_011531161.2
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A3 (MAGEA3), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531161.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGCCTCTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAAG AAGGCCTTGA GGCCCGAGGA 
GAGGCCCTGG GCCTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GGCTGCCTCC
TCCTCTTCTA CTCTAGTTGA AGTCACCCTG GGGGAGGTGC CTGCTGCCGA GTCACCAGAT
CCTCCCCAGA GTCCTCAGGG AGCCTCCAGC CTCCCCACTA CCATGAACTA CCCTCTCTGG
AGCCAATCCT ATGAGGACTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTTCCCTGAC
CTGGAGTCCG AGTTCCAAGC AGCACTCAGT AGGAAGGTGG CCGAGTTGGT TCATTTTCTG
CTCCTCAAGT ATCGAGCCAG GGAGCCGGTC ACAAAGGCAG AAATGCTGGG GAGTGTCGTC
GGAAATTGGC AGTATTTCTT TCCTGTGATC TTCAGCAAAG CTTCCAGTTC CTTGCAGCTG
GTCTTTGGCA TCGAGCTGAT GGAAGTGGAC CCCATCGGCC ACTTGTACAT CTTTGCCACC
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGTGACAATC AGATCATGCC CAAGGCAGGC
CTCCTGATAA TCGTCCTGGC CATAATCGCA AGAGAGGGCG ACTGTGCCCC TGAGGAGAAA
ATCTGGGAGG AGCTGAGTGT GTTAGAGGTG TTTGAGGGGA GGGAAGACAG TATCTTGGGG
GATCCCAAGA AGCTGCTCAC CCAACATTTC GTGCAGGAAA ACTACCTGGA GTACCGGCAG
GTCCCCGGCA GTGATCCTGC ATGTTATGAA TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA
ACCAGCTATG TGAAAGTCCT GCACCATATG GTAAAGATCA GTGGAGGACC TCACATTTCC
TACCCACCCC TGCATGAGTG GGTTTTGAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Degradation of AMPK by a cancer-specific ubiquitin ligase.
Cell160(4)715-28(2015 Feb)
Pineda CT,Ramanathan S,Fon Tacer K,Weon JL,Potts MB,Ou YH,White MA,Potts PR


book

MAGE proteins regulate KRAB zinc finger transcription factors and KAP1 E3 ligase activity.
Archives of biochemistry and biophysics563136-44(2014 Dec)
Xiao TZ,Suh Y,Longley BJ


book

Clinical significance of immunohistochemical expression of cancer/testis tumor-associated antigens (MAGE-A1, MAGE-A3/4, NY-ESO-1) in patients with non-small cell lung cancer.
Tumori100(1)60-8(2014 Jan-Feb)
Grah JJ,Katalinic D,Juretic A,Santek F,Samarzija M


book

Expression and clinical significance of cancer-testis genes in clear cell renal cell carcinoma.
International journal of clinical and experimental pathology7(7)4112-9(2014)
Yin B,Zeng Y,Wang X,Liu G,Zhang M,Song Y


book

MAGE-A3 is highly expressed in a cancer stem cell-like side population of bladder cancer cells.
International journal of clinical and experimental pathology7(6)2934-41(2014)
Yin B,Zeng Y,Liu G,Wang X,Wang P,Song Y


 
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