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MAGEA4 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MAGEA4
Entrez Gene ID 4103
Full Name MAGE family member A4
Synonyms CT1.4,MAGE-41,MAGE-X2,MAGE4,MAGE4A,MAGE4B
General protein information
Preferred Names

MAGE family member A4

Names

melanoma-associated antigen 4
MAGE-4 antigen
MAGE-41 antigen
MAGE-X2 antigen
cancer/testis antigen 1.4
cancer/testis antigen family 1, member 4
melanoma antigen family A, 4
melanoma antigen family A4

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

X

Xq28

Summary This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. At least four variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

300175


mRNA and Protein(s)

mRNA Protein Name
NM_001011550.1 NP_001011550.1 melanoma-associated antigen 4
NM_001011549.1 NP_001011549.1 melanoma-associated antigen 4
XM_005274679.3 XP_005274736.1 melanoma-associated antigen 4 isoform X2
XM_005274678.3 XP_005274735.1 melanoma-associated antigen 4 isoform X2
XM_005274677.2 XP_005274734.1 melanoma-associated antigen 4 isoform X1
NM_001011548.1 NP_001011548.1 melanoma-associated antigen 4
XM_005274679.2 XP_005274736.1 melanoma-associated antigen 4 isoform X1
NM_002362.4 NP_002353.3 melanoma-associated antigen 4



Pan troglodytes (chimpanzee) MAGEA4 XP_003317802.1
Macaca mulatta (rhesus monkey) MAGEA4 XP_001099496.2
Homo sapiens (human) MAGEA4 NP_001011548.1


Related articles in PubMed

Potential role of 5-aza-2'-deoxycytidine induced MAGE-A4 expression in immunotherapy for anaplastic thyroid cancer.
Gunda V, Cogdill AP, Bernasconi MJ, Wargo JA, Parangi S
Surgery154(6)1456-62; discussion 1462(2013 Dec)

Clinical significance of human leukocyte antigen loss and melanoma-associated antigen 4 expression in smokers of non-small cell lung cancer patients.
Baba T, Shiota H, Kuroda K, Shigematsu Y, Ichiki Y, Uramoto H, Hanagiri T, Tanaka F
International journal of clinical oncology18(6)997-1004(2013 Dec)

Proteomic profiling of triple-negative breast carcinomas in combination with a three-tier orthogonal technology approach identifies Mage-A4 as potential therapeutic target in estrogen receptor negative breast cancer.
Cabezón T, Gromova I, Gromov P, Serizawa R, Timmermans Wielenga V, Kroman N, Celis JE, Moreira JM
Molecular & cellular proteomics : MCP12(2)381-94(2013 Feb)

MAGE-4 gene m-RNA and TGF in blood as potential biochemical markers for HCC in HCV-infected patients.
Hussein YM, Morad FE, Gameel MA, Emam WA, El Sawy WH, El Tarhouny SA, Bayomy ES, Raafat N
Medical oncology (Northwood, London, England)29(5)3055-62(2012 Dec)

MAGEA4 induces growth in normal oral keratinocytes by inhibiting growth arrest and apoptosis.
Bhan S, Chuang A, Negi SS, Glazer CA, Califano JA
Oncology reports28(4)1498-502(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results found concomitant overexpression of MAGEA4 and TWIST1 in esophageal squamous cell carcinoma (ESCC). Also, it showed a correlation between MAGEA4 and TWIST1 expression, which was found as indirect binding of TWIST1 to the E-boxes in the MAGEA4 promoter revealing transcriptional upregulation of MAGEA4 by TWIST1.
Title: TWIST1 upregulates the MAGEA4 oncogene.

Results support the potential utility of NY-ESO-1, PRAME, and MAGEA4 as targets for immunotherapy and as ancillary prognostic parameters in synovial sarcomas.
Title: Cancer-testis antigen expression in synovial sarcoma: NY-ESO-1, PRAME, MAGEA4, and MAGEA1.

Data show that overall survival (OS) was significantly lower for patients expressing pan-MAGE or MAGE-A1/A3/A4 in both independent cohorts.
Title: MAGE expression in head and neck squamous cell carcinoma primary tumors, lymph node metastases and respective recurrences-implications for immunotherapy.

the crystal structures of MAGE-A3 and MAGE-A4
Title: Structures of Two Melanoma-Associated Antigens Suggest Allosteric Regulation of Effector Binding.

CTAs (MAGE-A4, NY-ESO-1, MAGE-A10) were more likely expressed in patients with squamous cell carcinoma of the lung and when CTAs combined with CD133, they can be better prognostic factors.
Title: Predictive and prognostic effect of CD133 and cancer-testis antigens in stage Ib-IIIA non-small cell lung cancer.

The following MAGEA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGEA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu02154 NM_001011550.1
Latest version!
Homo sapiens MAGE family member A4 (MAGEA4), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu02154 NM_001011549.1
Latest version!
Homo sapiens MAGE family member A4 (MAGEA4), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu02154 NM_001011548.1
Latest version!
Homo sapiens MAGE family member A4 (MAGEA4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu02154 NM_002362.4
Latest version!
Homo sapiens MAGE family member A4 (MAGEA4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu02154 XM_005274679.3
Latest version!
Homo sapiens MAGE family member A4 (MAGEA4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu02154 XM_005274678.3
Latest version!
Homo sapiens MAGE family member A4 (MAGEA4), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
OHu54401 XM_005274677.2
Latest version!
Homo sapiens MAGE family member A4 (MAGEA4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
View Old Accession Versions >>
OHu02154 XM_005274679.2 Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$99.50-$139.30
$199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu02154
Clone ID Related Accession (Same CDS sequence) NM_001011550.1 , NM_001011549.1 , XM_005274679.3 , XM_005274678.3 , NM_001011548.1 , XM_005274679.2 , NM_002362.4
Accession Version NM_001011550.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-15
Organism Homo sapiens(Human)
Product melanoma-associated antigen 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB120212.1 and AF274855.3. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017723.1, AK313862.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001011550.1
CDS207..1160
Misc Feature(1)219..491
Misc Feature(2)597..1049
Exon (1)1..69
Exon (2)70..141
Exon (3)142..1712
Translation

Target ORF information:

RefSeq Version NM_001011550.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A4 (MAGEA4), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001011550.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02154
Clone ID Related Accession (Same CDS sequence) NM_001011550.1 , NM_001011549.1 , XM_005274679.3 , XM_005274678.3 , NM_001011548.1 , XM_005274679.2 , NM_002362.4
Accession Version NM_001011549.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-15
Organism Homo sapiens(Human)
Product melanoma-associated antigen 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM548926.1 and AF274855.3. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM548926.1, BX361006.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001011549.1
CDS216..1169
Misc Feature(1)228..500
Misc Feature(2)606..1058
Exon (1)1..78
Exon (2)79..150
Exon (3)151..1721
Translation

Target ORF information:

RefSeq Version NM_001011549.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A4 (MAGEA4), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001011549.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02154
Clone ID Related Accession (Same CDS sequence) NM_001011550.1 , NM_001011549.1 , XM_005274679.3 , XM_005274678.3 , NM_001011548.1 , XM_005274679.2 , NM_002362.4
Accession Version NM_001011548.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-15
Organism Homo sapiens(Human)
Product melanoma-associated antigen 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX479311.1 and AF274855.3. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) has the 5'-most exon compared to variants 2-4. All four variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX479311.1, DA912058.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001011548.1
CDS219..1172
Misc Feature(1)36..38
Misc Feature(2)231..503
Misc Feature(3)609..1061
Exon (1)1..81
Exon (2)82..153
Exon (3)154..1724
Translation

Target ORF information:

RefSeq Version NM_001011548.1
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A4 (MAGEA4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001011548.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02154
Clone ID Related Accession (Same CDS sequence) NM_001011550.1 , NM_001011549.1 , XM_005274679.3 , XM_005274678.3 , NM_001011548.1 , XM_005274679.2 , NM_002362.4
Accession Version NM_002362.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-11-15
Organism Homo sapiens(Human)
Product melanoma-associated antigen 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM562570.1 and AF274855.3. On Feb 2, 2005 this sequence version replaced NM_002362.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. All four variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM548890.1, BX337705.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002353.3
CDS222..1175
Misc Feature(1)234..506
Misc Feature(2)612..1064
Exon (1)1..84
Exon (2)85..156
Exon (3)157..1727
Translation

Target ORF information:

RefSeq Version NM_002362.4
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A4 (MAGEA4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002362.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02154
Clone ID Related Accession (Same CDS sequence) NM_001011550.1 , NM_001011549.1 , XM_005274679.3 , XM_005274678.3 , NM_001011548.1 , XM_005274679.2 , NM_002362.4
Accession Version XM_005274679.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen 4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005274679.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274736.1
CDS229..1182
Misc Feature(1)241..513
Misc Feature(2)619..1071
Translation

Target ORF information:

RefSeq Version XM_005274679.3
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A4 (MAGEA4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274679.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02154
Clone ID Related Accession (Same CDS sequence) NM_001011550.1 , NM_001011549.1 , XM_005274679.3 , XM_005274678.3 , NM_001011548.1 , XM_005274679.2 , NM_002362.4
Accession Version XM_005274678.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen 4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005274678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274735.1
CDS220..1173
Misc Feature(1)232..504
Misc Feature(2)610..1062
Translation

Target ORF information:

RefSeq Version XM_005274678.3
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A4 (MAGEA4), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274678.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02154
Clone ID Related Accession (Same CDS sequence) NM_001011550.1 , NM_001011549.1 , XM_005274679.3 , XM_005274678.3 , NM_001011548.1 , XM_005274679.2 , NM_002362.4
Accession Version XM_005274679.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 954bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product melanoma-associated antigen 4 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274736.1
Translation

Target ORF information:

RefSeq Version XM_005274679.2
Organism Homo sapiens(Human)
Definition Homo sapiens melanoma antigen family A4 (MAGEA4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274679.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54401
Clone ID Related Accession (Same CDS sequence) XM_005274677.2
Accession Version XM_005274677.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1194bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product melanoma-associated antigen 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005274677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCCACTGA CTTGCGCATT GGGGGTTAGA GAGAAGCGAG CTGCTCTGAC CAGCCGCTTG 
GGATTGGCGG AGGGAAGCGG GCCAGGCCCT GTGAGGAGTC AAGGTTCTGA GCAGACAGGC
CAACCGGAGG ACAGGATTCC CTGGAGGCCA CAGAGGAGCA CCAAGGAGAA GATCTGCCTG
TGGGTCCCCA TTGCCCAGCT TTTGCCTGCA CTCTTGCCTG CTGCCCTGAC CAGAGTCATC
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274734.1
CDS22..1215
Misc Feature(1)274..546
Misc Feature(2)652..1104
Translation

Target ORF information:

RefSeq Version XM_005274677.2
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A4 (MAGEA4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274677.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGCCACTGA CTTGCGCATT GGGGGTTAGA GAGAAGCGAG CTGCTCTGAC CAGCCGCTTG 
GGATTGGCGG AGGGAAGCGG GCCAGGCCCT GTGAGGAGTC AAGGTTCTGA GCAGACAGGC
CAACCGGAGG ACAGGATTCC CTGGAGGCCA CAGAGGAGCA CCAAGGAGAA GATCTGCCTG
TGGGTCCCCA TTGCCCAGCT TTTGCCTGCA CTCTTGCCTG CTGCCCTGAC CAGAGTCATC
ATGTCTTCTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCGTTGA GGCCCAAGAA
GAGGCCCTGG GCCTGGTGGG TGCACAGGCT CCTACTACTG AGGAGCAGGA GGCTGCTGTC
TCCTCCTCCT CTCCTCTGGT CCCTGGCACC CTGGAGGAAG TGCCTGCTGC TGAGTCAGCA
GGTCCTCCCC AGAGTCCTCA GGGAGCCTCT GCCTTACCCA CTACCATCAG CTTCACTTGC
TGGAGGCAAC CCAATGAGGG TTCCAGCAGC CAAGAAGAGG AGGGGCCAAG CACCTCGCCT
GACGCAGAGT CCTTGTTCCG AGAAGCACTC AGTAACAAGG TGGATGAGTT GGCTCATTTT
CTGCTCCGCA AGTATCGAGC CAAGGAGCTG GTCACAAAGG CAGAAATGCT GGAGAGAGTC
ATCAAAAATT ACAAGCGCTG CTTTCCTGTG ATCTTCGGCA AAGCCTCCGA GTCCCTGAAG
ATGATCTTTG GCATTGACGT GAAGGAAGTG GACCCCGCCA GCAACACCTA CACCCTTGTC
ACCTGCCTGG GCCTTTCCTA TGATGGCCTG CTGGGTAATA ATCAGATCTT TCCCAAGACA
GGCCTTCTGA TAATCGTCCT GGGCACAATT GCAATGGAGG GCGACAGCGC CTCTGAGGAG
GAAATCTGGG AGGAGCTGGG TGTGATGGGG GTGTATGATG GGAGGGAGCA CACTGTCTAT
GGGGAGCCCA GGAAACTGCT CACCCAAGAT TGGGTGCAGG AAAACTACCT GGAGTACCGG
CAGGTACCCG GCAGTAATCC TGCGCGCTAT GAGTTCCTGT GGGGTCCAAG GGCTCTGGCT
GAAACCAGCT ATGTGAAAGT CCTGGAGCAT GTGGTCAGGG TCAATGCAAG AGTTCGCATT
GCCTACCCAT CCCTGCGTGA AGCAGCTTTG TTAGAGGAGG AAGAGGGAGT CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Potential role of 5-aza-2'-deoxycytidine induced MAGE-A4 expression in immunotherapy for anaplastic thyroid cancer.
Surgery154(6)1456-62; discussion 1462(2013 Dec)
Gunda V,Cogdill AP,Bernasconi MJ,Wargo JA,Parangi S


book

Clinical significance of human leukocyte antigen loss and melanoma-associated antigen 4 expression in smokers of non-small cell lung cancer patients.
International journal of clinical oncology18(6)997-1004(2013 Dec)
Baba T,Shiota H,Kuroda K,Shigematsu Y,Ichiki Y,Uramoto H,Hanagiri T,Tanaka F


book

Proteomic profiling of triple-negative breast carcinomas in combination with a three-tier orthogonal technology approach identifies Mage-A4 as potential therapeutic target in estrogen receptor negative breast cancer.
Molecular & cellular proteomics : MCP12(2)381-94(2013 Feb)
Cabezón T,Gromova I,Gromov P,Serizawa R,Timmermans Wielenga V,Kroman N,Celis JE,Moreira JM


book

MAGE-4 gene m-RNA and TGF in blood as potential biochemical markers for HCC in HCV-infected patients.
Medical oncology (Northwood, London, England)29(5)3055-62(2012 Dec)
Hussein YM,Morad FE,Gameel MA,Emam WA,El Sawy WH,El Tarhouny SA,Bayomy ES,Raafat N


book

MAGEA4 induces growth in normal oral keratinocytes by inhibiting growth arrest and apoptosis.
Oncology reports28(4)1498-502(2012 Oct)
Bhan S,Chuang A,Negi SS,Glazer CA,Califano JA


 
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