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MAGEA5 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MAGEA5
Entrez Gene ID 4104
Full Name MAGE family member A5
Synonyms CT1.5,MAGE5,MAGEA4
General protein information
Preferred Names

MAGE family member A5

Names

melanoma-associated antigen 5
MAGE-5 antigen
MAGE-5a antigen
MAGE-5b antigen
cancer/testis antigen 1.5
cancer/testis antigen family 1, member 5
melanoma antigen family A, 5
melanoma antigen family A5

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

X

Xq28

Summary This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This MAGEA gene encodes a protein that is C-terminally truncated compared to other family members, and this gene can be alternatively interpreted to be a pseudogene. The protein is represented in this Gene record in accordance with the assumed protein-coding status defined in the literature. Read-through transcription exists between this gene and the upstream melanoma antigen family A, 10 (MAGEA10) gene. [provided by RefSeq, Oct 2011].
Disorder MIM:

300340


mRNA and Protein(s)

mRNA Protein Name
NM_021049.4 NP_066387.1 melanoma-associated antigen 5



Homo sapiens (human) MAGEA5 NP_066387.1
Homo sapiens (human) MAGEA10-MAGEA5 NP_001191740.1


Related articles in PubMed

Multiple cancer testis antigens function to support tumor cell mitotic fidelity.
Cappell KM, Sinnott R, Taus P, Maxfield K, Scarbrough M, Whitehurst AW
Molecular and cellular biology32(20)4131-40(2012 Oct)

MAGE A1-A6 RT-PCR and MAGE A3 and p16 methylation analysis in induced sputum from patients with lung cancer and non-malignant lung diseases.
Shin KC, Lee KH, Lee CH, Shin IH, Suh HS, Jeon CH
Oncology reports27(4)911-6(2012 Apr)

Expression of melanoma-associated antigens in oral squamous cell carcinoma.
Ries J, Vairaktaris E, Mollaoglu N, Wiltfang J, Neukam FW, Nkenke E
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology37(2)88-93(2008 Feb)

Quantitative evaluation of the expression of MAGE genes in tumors by limiting dilution of cDNA libraries.
Serrano A, Lethé B, Delroisse JM, Lurquin C, De Plaen E, Brasseur F, Rimoldi D, Boon T
International journal of cancer83(5)664-9(1999 Nov)

The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28.
Rogner UC, Wilke K, Steck E, Korn B, Poustka A
Genomics29(3)725-31(1995 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report MAGEA1-A6 expression in sputum suggests presence of lung cancer cells or precancerous cells.
Title: MAGE A1-A6 RT-PCR and MAGE A3 and p16 methylation analysis in induced sputum from patients with lung cancer and non-malignant lung diseases.

Multiple simultaneous detection of MAGE-A [subtypes] more specific and sensitive than detection of single MAGE-A antigen for the diagnostic and prognostic evaluation of oral squamous cell carcinoma
Title: Expression of melanoma-associated antigens in oral squamous cell carcinoma.

The following MAGEA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGEA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu08405 NM_021049.4
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Homo sapiens MAGE family member A5 (MAGEA5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu08405
Clone ID Related Accession (Same CDS sequence) NM_021049.4
Accession Version NM_021049.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 375bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-29
Organism Homo sapiens(Human)
Product melanoma-associated antigen 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC109187.1, AF274856.3 and AI200443.1. This sequence is a reference standard in the RefSeqGene project. On Oct 18, 2011 this sequence version replaced NM_021049.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC109187.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2148874, SAMEA2149398 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGTCTCTTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA CACCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGTGCAGGCT GCCACTACTG AGGAGCAGGA GGCTGTGTCC
TCCTCCTCTC CTCTGGTCCC AGGCACCCTG GGGGAGGTGC CTGCTGCTGG GTCACCAGGT
CCTCTCAAGA GTCCTCAGGG AGCCTCCGCC ATCCCCACTG CCATCGATTT CACTCTATGG
AGGCAATCCA TTAAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTCCCCTGAC
CCAGAGTCTG TGTTCCGAGC AGCACTCAGT AAGAAGGTGG CTGACTTGAT TCATTTTCTG
CTCCTCAAGT ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_066387.1
CDS173..547
Misc Feature(1)38..40
Misc Feature(2)185..454
Exon (1)1..20
Exon (2)21..107
Exon (3)108..1664
Translation

Target ORF information:

RefSeq Version NM_021049.4
Organism Homo sapiens(Human)
Definition Homo sapiens MAGE family member A5 (MAGEA5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_021049.4

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGTCTCTTG AGCAGAAGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA CACCCAAGAA 
GAGGCCCTGG GCCTGGTGGG TGTGCAGGCT GCCACTACTG AGGAGCAGGA GGCTGTGTCC
TCCTCCTCTC CTCTGGTCCC AGGCACCCTG GGGGAGGTGC CTGCTGCTGG GTCACCAGGT
CCTCTCAAGA GTCCTCAGGG AGCCTCCGCC ATCCCCACTG CCATCGATTT CACTCTATGG
AGGCAATCCA TTAAGGGCTC CAGCAACCAA GAAGAGGAGG GGCCAAGCAC CTCCCCTGAC
CCAGAGTCTG TGTTCCGAGC AGCACTCAGT AAGAAGGTGG CTGACTTGAT TCATTTTCTG
CTCCTCAAGT ATTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Multiple cancer testis antigens function to support tumor cell mitotic fidelity.
Molecular and cellular biology32(20)4131-40(2012 Oct)
Cappell KM,Sinnott R,Taus P,Maxfield K,Scarbrough M,Whitehurst AW


book

MAGE A1-A6 RT-PCR and MAGE A3 and p16 methylation analysis in induced sputum from patients with lung cancer and non-malignant lung diseases.
Oncology reports27(4)911-6(2012 Apr)
Shin KC,Lee KH,Lee CH,Shin IH,Suh HS,Jeon CH


book

Expression of melanoma-associated antigens in oral squamous cell carcinoma.
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology37(2)88-93(2008 Feb)
Ries J,Vairaktaris E,Mollaoglu N,Wiltfang J,Neukam FW,Nkenke E


book

Quantitative evaluation of the expression of MAGE genes in tumors by limiting dilution of cDNA libraries.
International journal of cancer83(5)664-9(1999 Nov)
Serrano A,Lethé B,Delroisse JM,Lurquin C,De Plaen E,Brasseur F,Rimoldi D,Boon T


book

The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28.
Genomics29(3)725-31(1995 Oct)
Rogner UC,Wilke K,Steck E,Korn B,Poustka A


 
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