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MECP2 cDNA ORF clone, Homo sapiens (human)

Gene Symbol MECP2
Entrez Gene ID 4204
Full Name methyl CpG binding protein 2
Synonyms AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT
General protein information
Preferred Names
methyl-CpG-binding protein 2
Names
methyl-CpG-binding protein 2
meCp-2 protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

X

Xq28

Summary DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]. lac of sum
Disorder MIM:

300005

Disorder Html: Rett syndrome, 312750 (3); Mental retardation, X-linked, syndromic

mRNA and Protein(s)

mRNA Protein Name
XM_005274681 XP_005274738 methyl-CpG-binding protein 2 isoform X1
XM_005274683 XP_005274740 methyl-CpG-binding protein 2 isoform X2
XM_005274682 XP_005274739 methyl-CpG-binding protein 2 isoform X2
XM_011531165 XP_011529467 methyl-CpG-binding protein 2 isoform X2
XM_011531166 XP_011529468 methyl-CpG-binding protein 2 isoform X2
XM_006724819 XP_006724882 methyl-CpG-binding protein 2 isoform X3
NM_004992 NP_004983 methyl-CpG-binding protein 2 isoform 1
NM_001110792 NP_001104262 methyl-CpG-binding protein 2 isoform 2



Homo sapiens (human) MECP2 NP_004983.1
Pan troglodytes (chimpanzee) MECP2 XP_521333.3
Macaca mulatta (Rhesus monkey) MECP2 XP_001088566.2
Canis lupus familiaris (dog) MECP2 XP_853488.1
Bos taurus (cattle) MECP2 NP_001193481.1
Mus musculus (house mouse) Mecp2 NP_034918.1
Rattus norvegicus (Norway rat) Mecp2 NP_073164.2
Danio rerio (zebrafish) mecp2 NP_997901.1
Xenopus (Silurana) tropicalis (western clawed frog) mecp2 NP_001135563.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MECP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MECP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu28311
XM_005274681 PREDICTED: Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu54402 XM_005274683 PREDICTED: Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu54402 XM_005274682 PREDICTED: Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu54402 XM_011531165 PREDICTED: Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu54402 XM_011531166 PREDICTED: Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu54403 XM_006724819 PREDICTED: Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $139.30
$199.00
OHu28311
NM_004992 Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu28304
NM_001110792 Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu28311
Clone ID Related Accession (Same CDS sequence) NM_004992 , XM_005274681
Accession Version XM_005274681.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methyl-CpG-binding protein 2 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011681.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838853. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGTAGCTG GGATGTTAGG GCTCAGGGAA GAAAAGTCAG AAGACCAGGA CCTCCAGGGC 
CTCAAGGACA AACCCCTCAA GTTTAAAAAG GTGAAGAAAG ATAAGAAAGA AGAGAAAGAG
GGCAAGCATG AGCCCGTGCA GCCATCAGCC CACCACTCTG CTGAGCCCGC AGAGGCAGGC
AAAGCAGAGA CATCAGAAGG GTCAGGCTCC GCCCCGGCTG TGCCGGAAGC TTCTGCCTCC
CCCAAACAGC GGCGCTCCAT CATCCGTGAC CGGGGACCCA TGTATGATGA CCCCACCCTG
CCTGAAGGCT GGACACGGAA GCTTAAGCAA AGGAAATCTG GCCGCTCTGC TGGGAAGTAT
GATGTGTATT TGATCAATCC CCAGGGAAAA GCCTTTCGCT CTAAAGTGGA GTTGATTGCG
TACTTCGAAA AGGTAGGCGA CACATCCCTG GACCCTAATG ATTTTGACTT CACGGTAACT
GGGAGAGGGA GCCCCTCCCG GCGAGAGCAG AAACCACCTA AGAAGCCCAA ATCTCCCAAA
GCTCCAGGAA CTGGCAGAGG CCGGGGACGC CCCAAAGGGA GCGGCACCAC GAGACCCAAG
GCGGCCACGT CAGAGGGTGT GCAGGTGAAA AGGGTCCTGG AGAAAAGTCC TGGGAAGCTC
CTTGTCAAGA TGCCTTTTCA AACTTCGCCA GGGGGCAAGG CTGAGGGGGG TGGGGCCACC
ACATCCACCC AGGTCATGGT GATCAAACGC CCCGGCAGGA AGCGAAAAGC TGAGGCCGAC
CCTCAGGCCA TTCCCAAGAA ACGGGGCCGA AAGCCGGGGA GTGTGGTGGC AGCCGCTGCC
GCCGAGGCCA AAAAGAAAGC CGTGAAGGAG TCTTCTATCC GATCTGTGCA GGAGACCGTA
CTCCCCATCA AGAAGCGCAA GACCCGGGAG ACGGTCAGCA TCGAGGTCAA GGAAGTGGTG
AAGCCCCTGC TGGTGTCCAC CCTCGGTGAG AAGAGCGGGA AAGGACTGAA GACCTGTAAG
AGCCCTGGGC GGAAAAGCAA GGAGAGCAGC CCCAAGGGGC GCAGCAGCAG CGCCTCCTCA
CCCCCCAAGA AGGAGCACCA CCACCATCAC CACCACTCAG AGTCCCCAAA GGCCCCCGTG
CCACTGCTCC CACCCCTGCC CCCACCTCCA CCTGAGCCCG AGAGCTCCGA GGACCCCACC
AGCCCCCCTG AGCCCCAGGA CTTGAGCAGC AGCGTCTGCA AAGAGGAGAA GATGCCCAGA
GGAGGCTCAC TGGAGAGCGA CGGCTGCCCC AAGGAGCCAG CTAAGACTCA GCCCGCGGTT
GCCACCGCCG CCACGGCCGC AGAAAAGTAC AAACACCGAG GGGAGGGAGA GCGCAAAGAC
ATTGTTTCAT CCTCCATGCC AAGGCCAAAC AGAGAGGAGC CTGTGGACAG CCGGACGCCC
GTGACCGAGA GAGTTAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274738.1
CDS256..1716
Misc Feature(1)535..759(+)
Misc Feature(2)574..666(+)
Translation

Target ORF information:

RefSeq Version XM_005274681
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274681

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGTAGCTG GGATGTTAGG GCTCAGGGAA GAAAAGTCAG AAGACCAGGA CCTCCAGGGC 
CTCAAGGACA AACCCCTCAA GTTTAAAAAG GTGAAGAAAG ATAAGAAAGA AGAGAAAGAG
GGCAAGCATG AGCCCGTGCA GCCATCAGCC CACCACTCTG CTGAGCCCGC AGAGGCAGGC
AAAGCAGAGA CATCAGAAGG GTCAGGCTCC GCCCCGGCTG TGCCGGAAGC TTCTGCCTCC
CCCAAACAGC GGCGCTCCAT CATCCGTGAC CGGGGACCCA TGTATGATGA CCCCACCCTG
CCTGAAGGCT GGACACGGAA GCTTAAGCAA AGGAAATCTG GCCGCTCTGC TGGGAAGTAT
GATGTGTATT TGATCAATCC CCAGGGAAAA GCCTTTCGCT CTAAAGTGGA GTTGATTGCG
TACTTCGAAA AGGTAGGCGA CACATCCCTG GACCCTAATG ATTTTGACTT CACGGTAACT
GGGAGAGGGA GCCCCTCCCG GCGAGAGCAG AAACCACCTA AGAAGCCCAA ATCTCCCAAA
GCTCCAGGAA CTGGCAGAGG CCGGGGACGC CCCAAAGGGA GCGGCACCAC GAGACCCAAG
GCGGCCACGT CAGAGGGTGT GCAGGTGAAA AGGGTCCTGG AGAAAAGTCC TGGGAAGCTC
CTTGTCAAGA TGCCTTTTCA AACTTCGCCA GGGGGCAAGG CTGAGGGGGG TGGGGCCACC
ACATCCACCC AGGTCATGGT GATCAAACGC CCCGGCAGGA AGCGAAAAGC TGAGGCCGAC
CCTCAGGCCA TTCCCAAGAA ACGGGGCCGA AAGCCGGGGA GTGTGGTGGC AGCCGCTGCC
GCCGAGGCCA AAAAGAAAGC CGTGAAGGAG TCTTCTATCC GATCTGTGCA GGAGACCGTA
CTCCCCATCA AGAAGCGCAA GACCCGGGAG ACGGTCAGCA TCGAGGTCAA GGAAGTGGTG
AAGCCCCTGC TGGTGTCCAC CCTCGGTGAG AAGAGCGGGA AAGGACTGAA GACCTGTAAG
AGCCCTGGGC GGAAAAGCAA GGAGAGCAGC CCCAAGGGGC GCAGCAGCAG CGCCTCCTCA
CCCCCCAAGA AGGAGCACCA CCACCATCAC CACCACTCAG AGTCCCCAAA GGCCCCCGTG
CCACTGCTCC CACCCCTGCC CCCACCTCCA CCTGAGCCCG AGAGCTCCGA GGACCCCACC
AGCCCCCCTG AGCCCCAGGA CTTGAGCAGC AGCGTCTGCA AAGAGGAGAA GATGCCCAGA
GGAGGCTCAC TGGAGAGCGA CGGCTGCCCC AAGGAGCCAG CTAAGACTCA GCCCGCGGTT
GCCACCGCCG CCACGGCCGC AGAAAAGTAC AAACACCGAG GGGAGGGAGA GCGCAAAGAC
ATTGTTTCAT CCTCCATGCC AAGGCCAAAC AGAGAGGAGC CTGTGGACAG CCGGACGCCC
GTGACCGAGA GAGTTAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54402
Clone ID Related Accession (Same CDS sequence) XM_005274682 , XM_005274683 , XM_011531165 , XM_011531166
Accession Version XM_005274683.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methyl-CpG-binding protein 2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011681.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838855. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTATGATG ACCCCACCCT GCCTGAAGGC TGGACACGGA AGCTTAAGCA AAGGAAATCT 
GGCCGCTCTG CTGGGAAGTA TGATGTGTAT TTGATCAATC CCCAGGGAAA AGCCTTTCGC
TCTAAAGTGG AGTTGATTGC GTACTTCGAA AAGGTAGGCG ACACATCCCT GGACCCTAAT
GATTTTGACT TCACGGTAAC TGGGAGAGGG AGCCCCTCCC GGCGAGAGCA GAAACCACCT
AAGAAGCCCA AATCTCCCAA AGCTCCAGGA ACTGGCAGAG GCCGGGGACG CCCCAAAGGG
AGCGGCACCA CGAGACCCAA GGCGGCCACG TCAGAGGGTG TGCAGGTGAA AAGGGTCCTG
GAGAAAAGTC CTGGGAAGCT CCTTGTCAAG ATGCCTTTTC AAACTTCGCC AGGGGGCAAG
GCTGAGGGGG GTGGGGCCAC CACATCCACC CAGGTCATGG TGATCAAACG CCCCGGCAGG
AAGCGAAAAG CTGAGGCCGA CCCTCAGGCC ATTCCCAAGA AACGGGGCCG AAAGCCGGGG
AGTGTGGTGG CAGCCGCTGC CGCCGAGGCC AAAAAGAAAG CCGTGAAGGA GTCTTCTATC
CGATCTGTGC AGGAGACCGT ACTCCCCATC AAGAAGCGCA AGACCCGGGA GACGGTCAGC
ATCGAGGTCA AGGAAGTGGT GAAGCCCCTG CTGGTGTCCA CCCTCGGTGA GAAGAGCGGG
AAAGGACTGA AGACCTGTAA GAGCCCTGGG CGGAAAAGCA AGGAGAGCAG CCCCAAGGGG
CGCAGCAGCA GCGCCTCCTC ACCCCCCAAG AAGGAGCACC ACCACCATCA CCACCACTCA
GAGTCCCCAA AGGCCCCCGT GCCACTGCTC CCACCCCTGC CCCCACCTCC ACCTGAGCCC
GAGAGCTCCG AGGACCCCAC CAGCCCCCCT GAGCCCCAGG ACTTGAGCAG CAGCGTCTGC
AAAGAGGAGA AGATGCCCAG AGGAGGCTCA CTGGAGAGCG ACGGCTGCCC CAAGGAGCCA
GCTAAGACTC AGCCCGCGGT TGCCACCGCC GCCACGGCCG CAGAAAAGTA CAAACACCGA
GGGGAGGGAG AGCGCAAAGA CATTGTTTCA TCCTCCATGC CAAGGCCAAA CAGAGAGGAG
CCTGTGGACA GCCGGACGCC CGTGACCGAG AGAGTTAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274740.1
CDS792..1973
Misc Feature(1)792..1016(+)
Misc Feature(2)831..923(+)
Translation

Target ORF information:

RefSeq Version XM_005274683
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274683

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTATGATG ACCCCACCCT GCCTGAAGGC TGGACACGGA AGCTTAAGCA AAGGAAATCT 
GGCCGCTCTG CTGGGAAGTA TGATGTGTAT TTGATCAATC CCCAGGGAAA AGCCTTTCGC
TCTAAAGTGG AGTTGATTGC GTACTTCGAA AAGGTAGGCG ACACATCCCT GGACCCTAAT
GATTTTGACT TCACGGTAAC TGGGAGAGGG AGCCCCTCCC GGCGAGAGCA GAAACCACCT
AAGAAGCCCA AATCTCCCAA AGCTCCAGGA ACTGGCAGAG GCCGGGGACG CCCCAAAGGG
AGCGGCACCA CGAGACCCAA GGCGGCCACG TCAGAGGGTG TGCAGGTGAA AAGGGTCCTG
GAGAAAAGTC CTGGGAAGCT CCTTGTCAAG ATGCCTTTTC AAACTTCGCC AGGGGGCAAG
GCTGAGGGGG GTGGGGCCAC CACATCCACC CAGGTCATGG TGATCAAACG CCCCGGCAGG
AAGCGAAAAG CTGAGGCCGA CCCTCAGGCC ATTCCCAAGA AACGGGGCCG AAAGCCGGGG
AGTGTGGTGG CAGCCGCTGC CGCCGAGGCC AAAAAGAAAG CCGTGAAGGA GTCTTCTATC
CGATCTGTGC AGGAGACCGT ACTCCCCATC AAGAAGCGCA AGACCCGGGA GACGGTCAGC
ATCGAGGTCA AGGAAGTGGT GAAGCCCCTG CTGGTGTCCA CCCTCGGTGA GAAGAGCGGG
AAAGGACTGA AGACCTGTAA GAGCCCTGGG CGGAAAAGCA AGGAGAGCAG CCCCAAGGGG
CGCAGCAGCA GCGCCTCCTC ACCCCCCAAG AAGGAGCACC ACCACCATCA CCACCACTCA
GAGTCCCCAA AGGCCCCCGT GCCACTGCTC CCACCCCTGC CCCCACCTCC ACCTGAGCCC
GAGAGCTCCG AGGACCCCAC CAGCCCCCCT GAGCCCCAGG ACTTGAGCAG CAGCGTCTGC
AAAGAGGAGA AGATGCCCAG AGGAGGCTCA CTGGAGAGCG ACGGCTGCCC CAAGGAGCCA
GCTAAGACTC AGCCCGCGGT TGCCACCGCC GCCACGGCCG CAGAAAAGTA CAAACACCGA
GGGGAGGGAG AGCGCAAAGA CATTGTTTCA TCCTCCATGC CAAGGCCAAA CAGAGAGGAG
CCTGTGGACA GCCGGACGCC CGTGACCGAG AGAGTTAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54402
Clone ID Related Accession (Same CDS sequence) XM_005274682 , XM_005274683 , XM_011531165 , XM_011531166
Accession Version XM_005274682.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methyl-CpG-binding protein 2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011681.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838854. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTATGATG ACCCCACCCT GCCTGAAGGC TGGACACGGA AGCTTAAGCA AAGGAAATCT 
GGCCGCTCTG CTGGGAAGTA TGATGTGTAT TTGATCAATC CCCAGGGAAA AGCCTTTCGC
TCTAAAGTGG AGTTGATTGC GTACTTCGAA AAGGTAGGCG ACACATCCCT GGACCCTAAT
GATTTTGACT TCACGGTAAC TGGGAGAGGG AGCCCCTCCC GGCGAGAGCA GAAACCACCT
AAGAAGCCCA AATCTCCCAA AGCTCCAGGA ACTGGCAGAG GCCGGGGACG CCCCAAAGGG
AGCGGCACCA CGAGACCCAA GGCGGCCACG TCAGAGGGTG TGCAGGTGAA AAGGGTCCTG
GAGAAAAGTC CTGGGAAGCT CCTTGTCAAG ATGCCTTTTC AAACTTCGCC AGGGGGCAAG
GCTGAGGGGG GTGGGGCCAC CACATCCACC CAGGTCATGG TGATCAAACG CCCCGGCAGG
AAGCGAAAAG CTGAGGCCGA CCCTCAGGCC ATTCCCAAGA AACGGGGCCG AAAGCCGGGG
AGTGTGGTGG CAGCCGCTGC CGCCGAGGCC AAAAAGAAAG CCGTGAAGGA GTCTTCTATC
CGATCTGTGC AGGAGACCGT ACTCCCCATC AAGAAGCGCA AGACCCGGGA GACGGTCAGC
ATCGAGGTCA AGGAAGTGGT GAAGCCCCTG CTGGTGTCCA CCCTCGGTGA GAAGAGCGGG
AAAGGACTGA AGACCTGTAA GAGCCCTGGG CGGAAAAGCA AGGAGAGCAG CCCCAAGGGG
CGCAGCAGCA GCGCCTCCTC ACCCCCCAAG AAGGAGCACC ACCACCATCA CCACCACTCA
GAGTCCCCAA AGGCCCCCGT GCCACTGCTC CCACCCCTGC CCCCACCTCC ACCTGAGCCC
GAGAGCTCCG AGGACCCCAC CAGCCCCCCT GAGCCCCAGG ACTTGAGCAG CAGCGTCTGC
AAAGAGGAGA AGATGCCCAG AGGAGGCTCA CTGGAGAGCG ACGGCTGCCC CAAGGAGCCA
GCTAAGACTC AGCCCGCGGT TGCCACCGCC GCCACGGCCG CAGAAAAGTA CAAACACCGA
GGGGAGGGAG AGCGCAAAGA CATTGTTTCA TCCTCCATGC CAAGGCCAAA CAGAGAGGAG
CCTGTGGACA GCCGGACGCC CGTGACCGAG AGAGTTAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005274739.1
CDS574..1755
Misc Feature(1)574..798(+)
Misc Feature(2)613..705(+)
Translation

Target ORF information:

RefSeq Version XM_005274682
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005274682

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTATGATG ACCCCACCCT GCCTGAAGGC TGGACACGGA AGCTTAAGCA AAGGAAATCT 
GGCCGCTCTG CTGGGAAGTA TGATGTGTAT TTGATCAATC CCCAGGGAAA AGCCTTTCGC
TCTAAAGTGG AGTTGATTGC GTACTTCGAA AAGGTAGGCG ACACATCCCT GGACCCTAAT
GATTTTGACT TCACGGTAAC TGGGAGAGGG AGCCCCTCCC GGCGAGAGCA GAAACCACCT
AAGAAGCCCA AATCTCCCAA AGCTCCAGGA ACTGGCAGAG GCCGGGGACG CCCCAAAGGG
AGCGGCACCA CGAGACCCAA GGCGGCCACG TCAGAGGGTG TGCAGGTGAA AAGGGTCCTG
GAGAAAAGTC CTGGGAAGCT CCTTGTCAAG ATGCCTTTTC AAACTTCGCC AGGGGGCAAG
GCTGAGGGGG GTGGGGCCAC CACATCCACC CAGGTCATGG TGATCAAACG CCCCGGCAGG
AAGCGAAAAG CTGAGGCCGA CCCTCAGGCC ATTCCCAAGA AACGGGGCCG AAAGCCGGGG
AGTGTGGTGG CAGCCGCTGC CGCCGAGGCC AAAAAGAAAG CCGTGAAGGA GTCTTCTATC
CGATCTGTGC AGGAGACCGT ACTCCCCATC AAGAAGCGCA AGACCCGGGA GACGGTCAGC
ATCGAGGTCA AGGAAGTGGT GAAGCCCCTG CTGGTGTCCA CCCTCGGTGA GAAGAGCGGG
AAAGGACTGA AGACCTGTAA GAGCCCTGGG CGGAAAAGCA AGGAGAGCAG CCCCAAGGGG
CGCAGCAGCA GCGCCTCCTC ACCCCCCAAG AAGGAGCACC ACCACCATCA CCACCACTCA
GAGTCCCCAA AGGCCCCCGT GCCACTGCTC CCACCCCTGC CCCCACCTCC ACCTGAGCCC
GAGAGCTCCG AGGACCCCAC CAGCCCCCCT GAGCCCCAGG ACTTGAGCAG CAGCGTCTGC
AAAGAGGAGA AGATGCCCAG AGGAGGCTCA CTGGAGAGCG ACGGCTGCCC CAAGGAGCCA
GCTAAGACTC AGCCCGCGGT TGCCACCGCC GCCACGGCCG CAGAAAAGTA CAAACACCGA
GGGGAGGGAG AGCGCAAAGA CATTGTTTCA TCCTCCATGC CAAGGCCAAA CAGAGAGGAG
CCTGTGGACA GCCGGACGCC CGTGACCGAG AGAGTTAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54402
Clone ID Related Accession (Same CDS sequence) XM_005274682 , XM_005274683 , XM_011531165 , XM_011531166
Accession Version XM_011531165.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methyl-CpG-binding protein 2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011681.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTATGATG ACCCCACCCT GCCTGAAGGC TGGACACGGA AGCTTAAGCA AAGGAAATCT 
GGCCGCTCTG CTGGGAAGTA TGATGTGTAT TTGATCAATC CCCAGGGAAA AGCCTTTCGC
TCTAAAGTGG AGTTGATTGC GTACTTCGAA AAGGTAGGCG ACACATCCCT GGACCCTAAT
GATTTTGACT TCACGGTAAC TGGGAGAGGG AGCCCCTCCC GGCGAGAGCA GAAACCACCT
AAGAAGCCCA AATCTCCCAA AGCTCCAGGA ACTGGCAGAG GCCGGGGACG CCCCAAAGGG
AGCGGCACCA CGAGACCCAA GGCGGCCACG TCAGAGGGTG TGCAGGTGAA AAGGGTCCTG
GAGAAAAGTC CTGGGAAGCT CCTTGTCAAG ATGCCTTTTC AAACTTCGCC AGGGGGCAAG
GCTGAGGGGG GTGGGGCCAC CACATCCACC CAGGTCATGG TGATCAAACG CCCCGGCAGG
AAGCGAAAAG CTGAGGCCGA CCCTCAGGCC ATTCCCAAGA AACGGGGCCG AAAGCCGGGG
AGTGTGGTGG CAGCCGCTGC CGCCGAGGCC AAAAAGAAAG CCGTGAAGGA GTCTTCTATC
CGATCTGTGC AGGAGACCGT ACTCCCCATC AAGAAGCGCA AGACCCGGGA GACGGTCAGC
ATCGAGGTCA AGGAAGTGGT GAAGCCCCTG CTGGTGTCCA CCCTCGGTGA GAAGAGCGGG
AAAGGACTGA AGACCTGTAA GAGCCCTGGG CGGAAAAGCA AGGAGAGCAG CCCCAAGGGG
CGCAGCAGCA GCGCCTCCTC ACCCCCCAAG AAGGAGCACC ACCACCATCA CCACCACTCA
GAGTCCCCAA AGGCCCCCGT GCCACTGCTC CCACCCCTGC CCCCACCTCC ACCTGAGCCC
GAGAGCTCCG AGGACCCCAC CAGCCCCCCT GAGCCCCAGG ACTTGAGCAG CAGCGTCTGC
AAAGAGGAGA AGATGCCCAG AGGAGGCTCA CTGGAGAGCG ACGGCTGCCC CAAGGAGCCA
GCTAAGACTC AGCCCGCGGT TGCCACCGCC GCCACGGCCG CAGAAAAGTA CAAACACCGA
GGGGAGGGAG AGCGCAAAGA CATTGTTTCA TCCTCCATGC CAAGGCCAAA CAGAGAGGAG
CCTGTGGACA GCCGGACGCC CGTGACCGAG AGAGTTAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529467.1
CDS630..1811
Misc Feature(1)630..854(+)
Misc Feature(2)669..761(+)
Translation

Target ORF information:

RefSeq Version XM_011531165
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531165

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTATGATG ACCCCACCCT GCCTGAAGGC TGGACACGGA AGCTTAAGCA AAGGAAATCT 
GGCCGCTCTG CTGGGAAGTA TGATGTGTAT TTGATCAATC CCCAGGGAAA AGCCTTTCGC
TCTAAAGTGG AGTTGATTGC GTACTTCGAA AAGGTAGGCG ACACATCCCT GGACCCTAAT
GATTTTGACT TCACGGTAAC TGGGAGAGGG AGCCCCTCCC GGCGAGAGCA GAAACCACCT
AAGAAGCCCA AATCTCCCAA AGCTCCAGGA ACTGGCAGAG GCCGGGGACG CCCCAAAGGG
AGCGGCACCA CGAGACCCAA GGCGGCCACG TCAGAGGGTG TGCAGGTGAA AAGGGTCCTG
GAGAAAAGTC CTGGGAAGCT CCTTGTCAAG ATGCCTTTTC AAACTTCGCC AGGGGGCAAG
GCTGAGGGGG GTGGGGCCAC CACATCCACC CAGGTCATGG TGATCAAACG CCCCGGCAGG
AAGCGAAAAG CTGAGGCCGA CCCTCAGGCC ATTCCCAAGA AACGGGGCCG AAAGCCGGGG
AGTGTGGTGG CAGCCGCTGC CGCCGAGGCC AAAAAGAAAG CCGTGAAGGA GTCTTCTATC
CGATCTGTGC AGGAGACCGT ACTCCCCATC AAGAAGCGCA AGACCCGGGA GACGGTCAGC
ATCGAGGTCA AGGAAGTGGT GAAGCCCCTG CTGGTGTCCA CCCTCGGTGA GAAGAGCGGG
AAAGGACTGA AGACCTGTAA GAGCCCTGGG CGGAAAAGCA AGGAGAGCAG CCCCAAGGGG
CGCAGCAGCA GCGCCTCCTC ACCCCCCAAG AAGGAGCACC ACCACCATCA CCACCACTCA
GAGTCCCCAA AGGCCCCCGT GCCACTGCTC CCACCCCTGC CCCCACCTCC ACCTGAGCCC
GAGAGCTCCG AGGACCCCAC CAGCCCCCCT GAGCCCCAGG ACTTGAGCAG CAGCGTCTGC
AAAGAGGAGA AGATGCCCAG AGGAGGCTCA CTGGAGAGCG ACGGCTGCCC CAAGGAGCCA
GCTAAGACTC AGCCCGCGGT TGCCACCGCC GCCACGGCCG CAGAAAAGTA CAAACACCGA
GGGGAGGGAG AGCGCAAAGA CATTGTTTCA TCCTCCATGC CAAGGCCAAA CAGAGAGGAG
CCTGTGGACA GCCGGACGCC CGTGACCGAG AGAGTTAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54402
Clone ID Related Accession (Same CDS sequence) XM_005274682 , XM_005274683 , XM_011531165 , XM_011531166
Accession Version XM_011531166.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1182bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methyl-CpG-binding protein 2 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011681.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTATGATG ACCCCACCCT GCCTGAAGGC TGGACACGGA AGCTTAAGCA AAGGAAATCT 
GGCCGCTCTG CTGGGAAGTA TGATGTGTAT TTGATCAATC CCCAGGGAAA AGCCTTTCGC
TCTAAAGTGG AGTTGATTGC GTACTTCGAA AAGGTAGGCG ACACATCCCT GGACCCTAAT
GATTTTGACT TCACGGTAAC TGGGAGAGGG AGCCCCTCCC GGCGAGAGCA GAAACCACCT
AAGAAGCCCA AATCTCCCAA AGCTCCAGGA ACTGGCAGAG GCCGGGGACG CCCCAAAGGG
AGCGGCACCA CGAGACCCAA GGCGGCCACG TCAGAGGGTG TGCAGGTGAA AAGGGTCCTG
GAGAAAAGTC CTGGGAAGCT CCTTGTCAAG ATGCCTTTTC AAACTTCGCC AGGGGGCAAG
GCTGAGGGGG GTGGGGCCAC CACATCCACC CAGGTCATGG TGATCAAACG CCCCGGCAGG
AAGCGAAAAG CTGAGGCCGA CCCTCAGGCC ATTCCCAAGA AACGGGGCCG AAAGCCGGGG
AGTGTGGTGG CAGCCGCTGC CGCCGAGGCC AAAAAGAAAG CCGTGAAGGA GTCTTCTATC
CGATCTGTGC AGGAGACCGT ACTCCCCATC AAGAAGCGCA AGACCCGGGA GACGGTCAGC
ATCGAGGTCA AGGAAGTGGT GAAGCCCCTG CTGGTGTCCA CCCTCGGTGA GAAGAGCGGG
AAAGGACTGA AGACCTGTAA GAGCCCTGGG CGGAAAAGCA AGGAGAGCAG CCCCAAGGGG
CGCAGCAGCA GCGCCTCCTC ACCCCCCAAG AAGGAGCACC ACCACCATCA CCACCACTCA
GAGTCCCCAA AGGCCCCCGT GCCACTGCTC CCACCCCTGC CCCCACCTCC ACCTGAGCCC
GAGAGCTCCG AGGACCCCAC CAGCCCCCCT GAGCCCCAGG ACTTGAGCAG CAGCGTCTGC
AAAGAGGAGA AGATGCCCAG AGGAGGCTCA CTGGAGAGCG ACGGCTGCCC CAAGGAGCCA
GCTAAGACTC AGCCCGCGGT TGCCACCGCC GCCACGGCCG CAGAAAAGTA CAAACACCGA
GGGGAGGGAG AGCGCAAAGA CATTGTTTCA TCCTCCATGC CAAGGCCAAA CAGAGAGGAG
CCTGTGGACA GCCGGACGCC CGTGACCGAG AGAGTTAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011529468.1
CDS398..1579
Misc Feature(1)398..622(+)
Misc Feature(2)437..529(+)
Translation

Target ORF information:

RefSeq Version XM_011531166
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011531166

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
ATGTATGATG ACCCCACCCT GCCTGAAGGC TGGACACGGA AGCTTAAGCA AAGGAAATCT 
GGCCGCTCTG CTGGGAAGTA TGATGTGTAT TTGATCAATC CCCAGGGAAA AGCCTTTCGC
TCTAAAGTGG AGTTGATTGC GTACTTCGAA AAGGTAGGCG ACACATCCCT GGACCCTAAT
GATTTTGACT TCACGGTAAC TGGGAGAGGG AGCCCCTCCC GGCGAGAGCA GAAACCACCT
AAGAAGCCCA AATCTCCCAA AGCTCCAGGA ACTGGCAGAG GCCGGGGACG CCCCAAAGGG
AGCGGCACCA CGAGACCCAA GGCGGCCACG TCAGAGGGTG TGCAGGTGAA AAGGGTCCTG
GAGAAAAGTC CTGGGAAGCT CCTTGTCAAG ATGCCTTTTC AAACTTCGCC AGGGGGCAAG
GCTGAGGGGG GTGGGGCCAC CACATCCACC CAGGTCATGG TGATCAAACG CCCCGGCAGG
AAGCGAAAAG CTGAGGCCGA CCCTCAGGCC ATTCCCAAGA AACGGGGCCG AAAGCCGGGG
AGTGTGGTGG CAGCCGCTGC CGCCGAGGCC AAAAAGAAAG CCGTGAAGGA GTCTTCTATC
CGATCTGTGC AGGAGACCGT ACTCCCCATC AAGAAGCGCA AGACCCGGGA GACGGTCAGC
ATCGAGGTCA AGGAAGTGGT GAAGCCCCTG CTGGTGTCCA CCCTCGGTGA GAAGAGCGGG
AAAGGACTGA AGACCTGTAA GAGCCCTGGG CGGAAAAGCA AGGAGAGCAG CCCCAAGGGG
CGCAGCAGCA GCGCCTCCTC ACCCCCCAAG AAGGAGCACC ACCACCATCA CCACCACTCA
GAGTCCCCAA AGGCCCCCGT GCCACTGCTC CCACCCCTGC CCCCACCTCC ACCTGAGCCC
GAGAGCTCCG AGGACCCCAC CAGCCCCCCT GAGCCCCAGG ACTTGAGCAG CAGCGTCTGC
AAAGAGGAGA AGATGCCCAG AGGAGGCTCA CTGGAGAGCG ACGGCTGCCC CAAGGAGCCA
GCTAAGACTC AGCCCGCGGT TGCCACCGCC GCCACGGCCG CAGAAAAGTA CAAACACCGA
GGGGAGGGAG AGCGCAAAGA CATTGTTTCA TCCTCCATGC CAAGGCCAAA CAGAGAGGAG
CCTGTGGACA GCCGGACGCC CGTGACCGAG AGAGTTAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu54403
Clone ID Related Accession (Same CDS sequence) XM_006724819
Accession Version XM_006724819.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 792bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product methyl-CpG-binding protein 2 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_011681.17) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578838856. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCCTTTTC AAACTTCGCC AGGGGGCAAG GCTGAGGGGG GTGGGGCCAC CACATCCACC 
CAGGTCATGG TGATCAAACG CCCCGGCAGG AAGCGAAAAG CTGAGGCCGA CCCTCAGGCC
ATTCCCAAGA AACGGGGCCG AAAGCCGGGG AGTGTGGTGG CAGCCGCTGC CGCCGAGGCC
AAAAAGAAAG CCGTGAAGGA GTCTTCTATC CGATCTGTGC AGGAGACCGT ACTCCCCATC
AAGAAGCGCA AGACCCGGGA GACGGTCAGC ATCGAGGTCA AGGAAGTGGT GAAGCCCCTG
CTGGTGTCCA CCCTCGGTGA GAAGAGCGGG AAAGGACTGA AGACCTGTAA GAGCCCTGGG
CGGAAAAGCA AGGAGAGCAG CCCCAAGGGG CGCAGCAGCA GCGCCTCCTC ACCCCCCAAG
AAGGAGCACC ACCACCATCA CCACCACTCA GAGTCCCCAA AGGCCCCCGT GCCACTGCTC
CCACCCCTGC CCCCACCTCC ACCTGAGCCC GAGAGCTCCG AGGACCCCAC CAGCCCCCCT
GAGCCCCAGG ACTTGAGCAG CAGCGTCTGC AAAGAGGAGA AGATGCCCAG AGGAGGCTCA
CTGGAGAGCG ACGGCTGCCC CAAGGAGCCA GCTAAGACTC AGCCCGCGGT TGCCACCGCC
GCCACGGCCG CAGAAAAGTA CAAACACCGA GGGGAGGGAG AGCGCAAAGA CATTGTTTCA
TCCTCCATGC CAAGGCCAAA CAGAGAGGAG CCTGTGGACA GCCGGACGCC CGTGACCGAG
AGAGTTAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006724882.1
CDS201..992
Translation

Target ORF information:

RefSeq Version XM_006724819
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006724819

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGCCTTTTC AAACTTCGCC AGGGGGCAAG GCTGAGGGGG GTGGGGCCAC CACATCCACC 
CAGGTCATGG TGATCAAACG CCCCGGCAGG AAGCGAAAAG CTGAGGCCGA CCCTCAGGCC
ATTCCCAAGA AACGGGGCCG AAAGCCGGGG AGTGTGGTGG CAGCCGCTGC CGCCGAGGCC
AAAAAGAAAG CCGTGAAGGA GTCTTCTATC CGATCTGTGC AGGAGACCGT ACTCCCCATC
AAGAAGCGCA AGACCCGGGA GACGGTCAGC ATCGAGGTCA AGGAAGTGGT GAAGCCCCTG
CTGGTGTCCA CCCTCGGTGA GAAGAGCGGG AAAGGACTGA AGACCTGTAA GAGCCCTGGG
CGGAAAAGCA AGGAGAGCAG CCCCAAGGGG CGCAGCAGCA GCGCCTCCTC ACCCCCCAAG
AAGGAGCACC ACCACCATCA CCACCACTCA GAGTCCCCAA AGGCCCCCGT GCCACTGCTC
CCACCCCTGC CCCCACCTCC ACCTGAGCCC GAGAGCTCCG AGGACCCCAC CAGCCCCCCT
GAGCCCCAGG ACTTGAGCAG CAGCGTCTGC AAAGAGGAGA AGATGCCCAG AGGAGGCTCA
CTGGAGAGCG ACGGCTGCCC CAAGGAGCCA GCTAAGACTC AGCCCGCGGT TGCCACCGCC
GCCACGGCCG CAGAAAAGTA CAAACACCGA GGGGAGGGAG AGCGCAAAGA CATTGTTTCA
TCCTCCATGC CAAGGCCAAA CAGAGAGGAG CCTGTGGACA GCCGGACGCC CGTGACCGAG
AGAGTTAGCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28311
Clone ID Related Accession (Same CDS sequence) NM_004992 , XM_005274681
Accession Version NM_004992.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1461bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product methyl-CpG-binding protein 2 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB209464.1, AC244097.3 and AF158180.1. This sequence is a reference standard in the RefSeqGene project. On Nov 20, 2007 this sequence version replaced gi:7710148. Transcript Variant: This variant (1), also known as MECP2A, includes exon 2. Translation is reported to initiate in exon 2 resulting in a protein isoform (1) with a distinct N-terminus. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GQ203293.1, GQ203294.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2159368 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGTAGCTG GGATGTTAGG GCTCAGGGAA GAAAAGTCAG AAGACCAGGA CCTCCAGGGC 
CTCAAGGACA AACCCCTCAA GTTTAAAAAG GTGAAGAAAG ATAAGAAAGA AGAGAAAGAG
GGCAAGCATG AGCCCGTGCA GCCATCAGCC CACCACTCTG CTGAGCCCGC AGAGGCAGGC
AAAGCAGAGA CATCAGAAGG GTCAGGCTCC GCCCCGGCTG TGCCGGAAGC TTCTGCCTCC
CCCAAACAGC GGCGCTCCAT CATCCGTGAC CGGGGACCCA TGTATGATGA CCCCACCCTG
CCTGAAGGCT GGACACGGAA GCTTAAGCAA AGGAAATCTG GCCGCTCTGC TGGGAAGTAT
GATGTGTATT TGATCAATCC CCAGGGAAAA GCCTTTCGCT CTAAAGTGGA GTTGATTGCG
TACTTCGAAA AGGTAGGCGA CACATCCCTG GACCCTAATG ATTTTGACTT CACGGTAACT
GGGAGAGGGA GCCCCTCCCG GCGAGAGCAG AAACCACCTA AGAAGCCCAA ATCTCCCAAA
GCTCCAGGAA CTGGCAGAGG CCGGGGACGC CCCAAAGGGA GCGGCACCAC GAGACCCAAG
GCGGCCACGT CAGAGGGTGT GCAGGTGAAA AGGGTCCTGG AGAAAAGTCC TGGGAAGCTC
CTTGTCAAGA TGCCTTTTCA AACTTCGCCA GGGGGCAAGG CTGAGGGGGG TGGGGCCACC
ACATCCACCC AGGTCATGGT GATCAAACGC CCCGGCAGGA AGCGAAAAGC TGAGGCCGAC
CCTCAGGCCA TTCCCAAGAA ACGGGGCCGA AAGCCGGGGA GTGTGGTGGC AGCCGCTGCC
GCCGAGGCCA AAAAGAAAGC CGTGAAGGAG TCTTCTATCC GATCTGTGCA GGAGACCGTA
CTCCCCATCA AGAAGCGCAA GACCCGGGAG ACGGTCAGCA TCGAGGTCAA GGAAGTGGTG
AAGCCCCTGC TGGTGTCCAC CCTCGGTGAG AAGAGCGGGA AAGGACTGAA GACCTGTAAG
AGCCCTGGGC GGAAAAGCAA GGAGAGCAGC CCCAAGGGGC GCAGCAGCAG CGCCTCCTCA
CCCCCCAAGA AGGAGCACCA CCACCATCAC CACCACTCAG AGTCCCCAAA GGCCCCCGTG
CCACTGCTCC CACCCCTGCC CCCACCTCCA CCTGAGCCCG AGAGCTCCGA GGACCCCACC
AGCCCCCCTG AGCCCCAGGA CTTGAGCAGC AGCGTCTGCA AAGAGGAGAA GATGCCCAGA
GGAGGCTCAC TGGAGAGCGA CGGCTGCCCC AAGGAGCCAG CTAAGACTCA GCCCGCGGTT
GCCACCGCCG CCACGGCCGC AGAAAAGTAC AAACACCGAG GGGAGGGAGA GCGCAAAGAC
ATTGTTTCAT CCTCCATGCC AAGGCCAAAC AGAGAGGAGC CTGTGGACAG CCGGACGCCC
GTGACCGAGA GAGTTAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004983.1
CDS227..1687
Misc Feature(1)176..178(+)
Misc Feature(2)464..466(+)
Misc Feature(3)464..466(+)
Misc Feature(4)506..730(+)
Misc Feature(5)545..637(+)
Misc Feature(6)572..574(+)
Misc Feature(7)872..874(+)
Misc Feature(8)1502..1504(+)
Misc Feature(9)1571..1573(+)
Exon (1)1..128
Gene:MECP2
Gene Synonym:
Exon (2)129..252
Gene:MECP2
Gene Synonym:
Exon (3)253..603
Gene:MECP2
Gene Synonym:
Exon (4)604..10241
Gene:MECP2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_004992
Organism Homo sapiens (human)
Definition Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004992

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGTAGCTG GGATGTTAGG GCTCAGGGAA GAAAAGTCAG AAGACCAGGA CCTCCAGGGC 
CTCAAGGACA AACCCCTCAA GTTTAAAAAG GTGAAGAAAG ATAAGAAAGA AGAGAAAGAG
GGCAAGCATG AGCCCGTGCA GCCATCAGCC CACCACTCTG CTGAGCCCGC AGAGGCAGGC
AAAGCAGAGA CATCAGAAGG GTCAGGCTCC GCCCCGGCTG TGCCGGAAGC TTCTGCCTCC
CCCAAACAGC GGCGCTCCAT CATCCGTGAC CGGGGACCCA TGTATGATGA CCCCACCCTG
CCTGAAGGCT GGACACGGAA GCTTAAGCAA AGGAAATCTG GCCGCTCTGC TGGGAAGTAT
GATGTGTATT TGATCAATCC CCAGGGAAAA GCCTTTCGCT CTAAAGTGGA GTTGATTGCG
TACTTCGAAA AGGTAGGCGA CACATCCCTG GACCCTAATG ATTTTGACTT CACGGTAACT
GGGAGAGGGA GCCCCTCCCG GCGAGAGCAG AAACCACCTA AGAAGCCCAA ATCTCCCAAA
GCTCCAGGAA CTGGCAGAGG CCGGGGACGC CCCAAAGGGA GCGGCACCAC GAGACCCAAG
GCGGCCACGT CAGAGGGTGT GCAGGTGAAA AGGGTCCTGG AGAAAAGTCC TGGGAAGCTC
CTTGTCAAGA TGCCTTTTCA AACTTCGCCA GGGGGCAAGG CTGAGGGGGG TGGGGCCACC
ACATCCACCC AGGTCATGGT GATCAAACGC CCCGGCAGGA AGCGAAAAGC TGAGGCCGAC
CCTCAGGCCA TTCCCAAGAA ACGGGGCCGA AAGCCGGGGA GTGTGGTGGC AGCCGCTGCC
GCCGAGGCCA AAAAGAAAGC CGTGAAGGAG TCTTCTATCC GATCTGTGCA GGAGACCGTA
CTCCCCATCA AGAAGCGCAA GACCCGGGAG ACGGTCAGCA TCGAGGTCAA GGAAGTGGTG
AAGCCCCTGC TGGTGTCCAC CCTCGGTGAG AAGAGCGGGA AAGGACTGAA GACCTGTAAG
AGCCCTGGGC GGAAAAGCAA GGAGAGCAGC CCCAAGGGGC GCAGCAGCAG CGCCTCCTCA
CCCCCCAAGA AGGAGCACCA CCACCATCAC CACCACTCAG AGTCCCCAAA GGCCCCCGTG
CCACTGCTCC CACCCCTGCC CCCACCTCCA CCTGAGCCCG AGAGCTCCGA GGACCCCACC
AGCCCCCCTG AGCCCCAGGA CTTGAGCAGC AGCGTCTGCA AAGAGGAGAA GATGCCCAGA
GGAGGCTCAC TGGAGAGCGA CGGCTGCCCC AAGGAGCCAG CTAAGACTCA GCCCGCGGTT
GCCACCGCCG CCACGGCCGC AGAAAAGTAC AAACACCGAG GGGAGGGAGA GCGCAAAGAC
ATTGTTTCAT CCTCCATGCC AAGGCCAAAC AGAGAGGAGC CTGTGGACAG CCGGACGCCC
GTGACCGAGA GAGTTAGCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28304
Clone ID Related Accession (Same CDS sequence) NM_001110792
Accession Version NM_001110792.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1497bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product methyl-CpG-binding protein 2 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB209464.1, BX538060.1 and BC011612.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2), also known as MECP2B, lacks exon 2. Translation is reported to initiate in the first exon resulting in a protein isoform (2) with a distinct N-terminus. This transcript is reported to be abundant in the central nervous system (PMID: 15034579, 17171659). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX538060.1, BI458175.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCCGCCG CCGCCGCCGC CGCGCCGAGC GGAGGAGGAG GAGGAGGCGA GGAGGAGAGA 
CTGGAAGAAA AGTCAGAAGA CCAGGACCTC CAGGGCCTCA AGGACAAACC CCTCAAGTTT
AAAAAGGTGA AGAAAGATAA GAAAGAAGAG AAAGAGGGCA AGCATGAGCC CGTGCAGCCA
TCAGCCCACC ACTCTGCTGA GCCCGCAGAG GCAGGCAAAG CAGAGACATC AGAAGGGTCA
GGCTCCGCCC CGGCTGTGCC GGAAGCTTCT GCCTCCCCCA AACAGCGGCG CTCCATCATC
CGTGACCGGG GACCCATGTA TGATGACCCC ACCCTGCCTG AAGGCTGGAC ACGGAAGCTT
AAGCAAAGGA AATCTGGCCG CTCTGCTGGG AAGTATGATG TGTATTTGAT CAATCCCCAG
GGAAAAGCCT TTCGCTCTAA AGTGGAGTTG ATTGCGTACT TCGAAAAGGT AGGCGACACA
TCCCTGGACC CTAATGATTT TGACTTCACG GTAACTGGGA GAGGGAGCCC CTCCCGGCGA
GAGCAGAAAC CACCTAAGAA GCCCAAATCT CCCAAAGCTC CAGGAACTGG CAGAGGCCGG
GGACGCCCCA AAGGGAGCGG CACCACGAGA CCCAAGGCGG CCACGTCAGA GGGTGTGCAG
GTGAAAAGGG TCCTGGAGAA AAGTCCTGGG AAGCTCCTTG TCAAGATGCC TTTTCAAACT
TCGCCAGGGG GCAAGGCTGA GGGGGGTGGG GCCACCACAT CCACCCAGGT CATGGTGATC
AAACGCCCCG GCAGGAAGCG AAAAGCTGAG GCCGACCCTC AGGCCATTCC CAAGAAACGG
GGCCGAAAGC CGGGGAGTGT GGTGGCAGCC GCTGCCGCCG AGGCCAAAAA GAAAGCCGTG
AAGGAGTCTT CTATCCGATC TGTGCAGGAG ACCGTACTCC CCATCAAGAA GCGCAAGACC
CGGGAGACGG TCAGCATCGA GGTCAAGGAA GTGGTGAAGC CCCTGCTGGT GTCCACCCTC
GGTGAGAAGA GCGGGAAAGG ACTGAAGACC TGTAAGAGCC CTGGGCGGAA AAGCAAGGAG
AGCAGCCCCA AGGGGCGCAG CAGCAGCGCC TCCTCACCCC CCAAGAAGGA GCACCACCAC
CATCACCACC ACTCAGAGTC CCCAAAGGCC CCCGTGCCAC TGCTCCCACC CCTGCCCCCA
CCTCCACCTG AGCCCGAGAG CTCCGAGGAC CCCACCAGCC CCCCTGAGCC CCAGGACTTG
AGCAGCAGCG TCTGCAAAGA GGAGAAGATG CCCAGAGGAG GCTCACTGGA GAGCGACGGC
TGCCCCAAGG AGCCAGCTAA GACTCAGCCC GCGGTTGCCA CCGCCGCCAC GGCCGCAGAA
AAGTACAAAC ACCGAGGGGA GGGAGAGCGC AAAGACATTG TTTCATCCTC CATGCCAAGG
CCAAACAGAG AGGAGCCTGT GGACAGCCGG ACGCCCGTGA CCGAGAGAGT TAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001104262.1
CDS67..1563
Misc Feature(1)382..606(+)
Misc Feature(2)421..513(+)
Exon (1)1..128
Gene:MECP2
Gene Synonym:
Exon (2)129..479
Gene:MECP2
Gene Synonym:
Exon (3)480..1696
Gene:MECP2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001110792
Organism Homo sapiens (human)
Definition Homo sapiens methyl CpG binding protein 2 (MECP2), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001110792

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGCCGCCG CCGCCGCCGC CGCGCCGAGC GGAGGAGGAG GAGGAGGCGA GGAGGAGAGA 
CTGGAAGAAA AGTCAGAAGA CCAGGACCTC CAGGGCCTCA AGGACAAACC CCTCAAGTTT
AAAAAGGTGA AGAAAGATAA GAAAGAAGAG AAAGAGGGCA AGCATGAGCC CGTGCAGCCA
TCAGCCCACC ACTCTGCTGA GCCCGCAGAG GCAGGCAAAG CAGAGACATC AGAAGGGTCA
GGCTCCGCCC CGGCTGTGCC GGAAGCTTCT GCCTCCCCCA AACAGCGGCG CTCCATCATC
CGTGACCGGG GACCCATGTA TGATGACCCC ACCCTGCCTG AAGGCTGGAC ACGGAAGCTT
AAGCAAAGGA AATCTGGCCG CTCTGCTGGG AAGTATGATG TGTATTTGAT CAATCCCCAG
GGAAAAGCCT TTCGCTCTAA AGTGGAGTTG ATTGCGTACT TCGAAAAGGT AGGCGACACA
TCCCTGGACC CTAATGATTT TGACTTCACG GTAACTGGGA GAGGGAGCCC CTCCCGGCGA
GAGCAGAAAC CACCTAAGAA GCCCAAATCT CCCAAAGCTC CAGGAACTGG CAGAGGCCGG
GGACGCCCCA AAGGGAGCGG CACCACGAGA CCCAAGGCGG CCACGTCAGA GGGTGTGCAG
GTGAAAAGGG TCCTGGAGAA AAGTCCTGGG AAGCTCCTTG TCAAGATGCC TTTTCAAACT
TCGCCAGGGG GCAAGGCTGA GGGGGGTGGG GCCACCACAT CCACCCAGGT CATGGTGATC
AAACGCCCCG GCAGGAAGCG AAAAGCTGAG GCCGACCCTC AGGCCATTCC CAAGAAACGG
GGCCGAAAGC CGGGGAGTGT GGTGGCAGCC GCTGCCGCCG AGGCCAAAAA GAAAGCCGTG
AAGGAGTCTT CTATCCGATC TGTGCAGGAG ACCGTACTCC CCATCAAGAA GCGCAAGACC
CGGGAGACGG TCAGCATCGA GGTCAAGGAA GTGGTGAAGC CCCTGCTGGT GTCCACCCTC
GGTGAGAAGA GCGGGAAAGG ACTGAAGACC TGTAAGAGCC CTGGGCGGAA AAGCAAGGAG
AGCAGCCCCA AGGGGCGCAG CAGCAGCGCC TCCTCACCCC CCAAGAAGGA GCACCACCAC
CATCACCACC ACTCAGAGTC CCCAAAGGCC CCCGTGCCAC TGCTCCCACC CCTGCCCCCA
CCTCCACCTG AGCCCGAGAG CTCCGAGGAC CCCACCAGCC CCCCTGAGCC CCAGGACTTG
AGCAGCAGCG TCTGCAAAGA GGAGAAGATG CCCAGAGGAG GCTCACTGGA GAGCGACGGC
TGCCCCAAGG AGCCAGCTAA GACTCAGCCC GCGGTTGCCA CCGCCGCCAC GGCCGCAGAA
AAGTACAAAC ACCGAGGGGA GGGAGAGCGC AAAGACATTG TTTCATCCTC CATGCCAAGG
CCAAACAGAG AGGAGCCTGT GGACAGCCGG ACGCCCGTGA CCGAGAGAGT TAGCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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