MAP3K5 cDNA ORF clone, Homo sapiens(human)

The following MAP3K5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu19594 NM_005923.4
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
OHu96613 XM_017010876.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu96614 XM_017010877.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu96606 XM_017010870.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu96607 XM_017010871.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu96608 XM_017010872.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu96609 XM_017010873.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu96610 XM_017010874.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu96611 XM_017010875.1
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $727.30
$1039.00
OHu96612 XM_011535839.3
Latest version!
Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
View Old Accession Versions >>
OHu96612 XM_011535839.2 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu19594 NM_005923.3 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$519.50-$727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu19594
    Clone ID Related Accession (Same CDS sequence) NM_005923.3 , NM_005923.4
    Accession Version NM_005923.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U67156.1. This sequence is a reference standard in the RefSeqGene project. On Jun 21, 2002 this sequence version replaced NM_005923.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U67156.1, SRR1803612.276620.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCACGG AGGCGGACGA GGGCATCACT TTCTCTGTGC CACCCTTCGC CCCCTCGGGC 
    TTCTGCACCA TCCCCGAGGG CGGCATCTGC AGGAGGGGAG GAGCGGCGGC GGTGGGCGAG
    GGCGAGGAGC ACCAGCTGCC ACCGCCGCCG CCGGGCAGCT TCTGGAACGT GGAGAGCGCC
    GCTGCCCCTG GCATCGGTTG TCCGGCGGCC ACCTCCTCGA GCAGTGCCAC CCGAGGCCGG
    GGCAGCTCTG TTGGCGGGGG CAGCCGACGG ACCACGGTGG CATATGTGAT CAACGAAGCG
    AGCCAAGGGC AACTGGTGGT GGCCGAGAGC GAGGCCCTGC AGAGCTTGCG GGAGGCGTGC
    GAGACAGTGG GCGCCACCCT GGAAACCCTG CATTTTGGGA AACTCGACTT TGGAGAAACC
    ACCGTGCTGG ACCGCTTTTA CAATGCAGAT ATTGCGGTGG TGGAGATGAG CGATGCCTTC
    CGGCAGCCGT CCTTGTTTTA CCACCTTGGG GTGAGAGAAA GTTTCAGCAT GGCCAACAAC
    ATCATCCTCT ACTGTGATAC TAACTCGGAC TCTCTGCAGT CACTGAAGGA AATAATTTGC
    CAGAAGAATA CTATGTGCAC TGGGAACTAC ACCTTTGTTC CTTACATGAT AACTCCACAT
    AACAAAGTCT ACTGCTGTGA CAGCAGCTTC ATGAAGGGGT TGACAGAGCT CATGCAACCG
    AACTTCGAGC TGCTTCTTGG ACCCATCTGC TTACCTCTTG TGGATCGTTT TATTCAACTT
    TTGAAGGTGG CACAAGCAAG TTCTAGCCAG TACTTCCGGG AATCTATACT CAATGACATC
    AGGAAAGCTC GTAATTTATA CACTGGTAAA GAATTGGCAG CTGAGTTGGC AAGAATTCGG
    CAGCGAGTAG ATAATATCGA AGTCTTGACA GCAGATATTG TCATAAATCT GTTACTTTCC
    TACAGAGATA TCCAGGACTA TGATTCTATT GTGAAGCTGG TAGAGACTTT AGAAAAACTG
    CCAACCTTTG ATTTGGCCTC CCATCACCAT GTGAAGTTTC ATTATGCATT TGCACTGAAT
    AGGAGAAATC TCCCTGGTGA CAGAGCAAAA GCTCTTGATA TTATGATTCC CATGGTGCAA
    AGCGAAGGAC AAGTTGCTTC AGATATGTAT TGCCTAGTTG GTCGAATCTA CAAAGATATG
    TTTTTGGACT CTAATTTCAC GGACACTGAA AGCAGAGACC ATGGAGCTTC TTGGTTCAAA
    AAGGCATTTG AATCTGAGCC AACACTACAG TCAGGAATTA ATTATGCGGT CCTCCTCCTG
    GCAGCTGGAC ACCAGTTTGA ATCTTCCTTT GAGCTCCGGA AAGTTGGGGT GAAGCTAAGT
    AGTCTTCTTG GTAAAAAGGG AAACTTGGAA AAACTCCAGA GCTACTGGGA AGTTGGATTT
    TTTCTGGGGG CCAGCGTCCT AGCCAATGAC CACATGAGAG TCATTCAAGC ATCTGAAAAG
    CTTTTTAAAC TGAAGACACC AGCATGGTAC CTCAAGTCTA TTGTAGAGAC AATTTTAATA
    TATAAGCATT TTGTGAAACT GACCACAGAA CAGCCTGTGG CCAAGCAAGA ACTTGTGGAC
    TTTTGGATGG ATTTCCTGGT CGAGGCCACA AAGACAGATG TTACTGTGGT TAGGTTTCCA
    GTATTAATAT TAGAACCAAC CAAAATCTAT CAACCTTCTT ATTTGTCTAT CAACAATGAA
    GTTGAGGAAA AGACAATCTC TATTTGGCAC GTGCTTCCTG ATGACAAGAA AGGTATACAT
    GAGTGGAATT TTAGTGCCTC TTCTGTCAGG GGAGTGAGTA TTTCTAAATT TGAAGAAAGA
    TGCTGCTTTC TTTATGTGCT TCACAATTCT GATGATTTCC AAATCTATTT CTGTACAGAA
    CTTCATTGTA AAAAGTTTTT TGAGATGGTG AACACCATTA CCGAAGAGAA GGGGAGAAGC
    ACAGAGGAAG GAGACTGTGA AAGTGACTTG CTGGAGTATG ACTATGAATA TGATGAAAAT
    GGTGACAGAG TCGTTTTAGG AAAAGGCACT TATGGGATAG TCTACGCAGG TCGGGACTTG
    AGCAACCAAG TCAGAATTGC TATTAAGGAA ATCCCAGAGA GAGACAGCAG ATACTCTCAG
    CCCCTGCATG AAGAAATAGC ATTGCATAAA CACCTGAAGC ACAAAAATAT TGTCCAGTAT
    CTGGGCTCTT TCAGTGAGAA TGGTTTCATT AAAATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTTTCTG CTCTCCTTCG TTCCAAATGG GGTCCATTAA AAGACAATGA GCAAACAATT
    GGCTTTTATA CAAAGCAAAT ACTGGAAGGA TTAAAATATC TCCATGACAA TCAGATAGTT
    CACCGGGACA TAAAGGGTGA CAATGTGTTG ATTAATACCT ACAGTGGTGT TCTCAAGATC
    TCTGACTTCG GAACATCAAA GAGGCTTGCT GGCATAAACC CCTGTACTGA AACTTTTACT
    GGTACCCTCC AGTATATGGC ACCAGAAATA ATAGATAAAG GACCAAGAGG CTACGGAAAA
    GCAGCAGACA TCTGGTCTCT GGGCTGTACA ATCATTGAAA TGGCCACAGG AAAACCCCCA
    TTTTATGAAC TGGGAGAACC ACAAGCAGCT ATGTTCAAGG TGGGAATGTT TAAAGTCCAC
    CCTGAGATCC CAGAGTCCAT GTCTGCAGAG GCCAAGGCAT TCATACTGAA ATGTTTTGAA
    CCAGATCCTG ACAAGAGAGC CTGTGCTAAC GACTTGCTTG TTGATGAGTT TTTAAAAGTT
    TCAAGCAAAA AGAAAAAGAC ACAACCTAAG CTTTCAGCTC TTTCAGCTGG ATCAAATGAA
    TATCTCAGGA GTATATCCTT GCCGGTACCT GTGCTGGTGG AGGACACCAG CAGCAGCAGT
    GAGTACGGCT CAGTTTCACC CGACACGGAG TTGAAAGTGG ACCCCTTCTC TTTCAAAACA
    AGAGCCAAGT CCTGCGGAGA AAGAGATGTC AAGGGAATTC GGACACTCTT TTTGGGCATT
    CCAGATGAGA ATTTTGAAGA TCACAGTGCT CCTCCTTCCC CTGAAGAAAA AGATTCTGGA
    TTCTTCATGC TGAGGAAGGA CAGTGAGAGG CGAGCTACCC TTCACAGGAT CCTGACGGAA
    GACCAAGACA AAATTGTGAG AAACCTAATG GAATCTTTAG CTCAGGGGGC TGAAGAACCG
    AAACTAAAAT GGGAACACAT CACAACCCTC ATTGCAAGCC TCAGAGAATT TGTGAGATCC
    ACTGACCGAA AAATCATAGC CACCACACTG TCAAAGCTGA AACTGGAGCT GGACTTCGAC
    AGCCATGGCA TTAGCCAAGT CCAGGTGGTA CTCTTTGGTT TTCAAGATGC TGTCAATAAA
    GTTCTTCGGA ATCATAACAT CAAGCCGCAC TGGATGTTTG CCTTAGACAG TATCATTCGG
    AAGGCGGTAC AGACAGCCAT TACCATCCTG GTTCCAGAAC TAAGGCCACA TTTCAGCCTT
    GCATCTGAGA GTGATACTGC TGATCAAGAA GACTTGGATG TAGAAGATGA CCATGAGGAA
    CAGCCTTCAA ATCAAACTGT CCGAAGACCT CAGGCTGTCA TTGAAGATGC TGTGGCTACC
    TCAGGCGTGA GCACGCTCAG TTCTACTGTG TCTCATGATT CCCAGAGTGC TCACCGGTCA
    CTGAATGTAC AGCTTGGAAG GATGAAAATA GAAACCAATA GATTACTGGA AGAATTGGTT
    CGGAAAGAGA AAGAATTACA AGCACTCCTT CATCGAGCTA TTGAAGAAAA AGACCAAGAA
    ATTAAACACC TGAAGCTTAA GTCCCAACCC ATAGAAATTC CTGAATTGCC TGTATTTCAT
    CTAAATTCTT CTGGCACAAA TACTGAAGAT TCTGAACTTA CCGACTGGCT GAGAGTGAAT
    GGAGCTGATG AAGACACTAT AAGCCGGTTT TTGGCTGAAG ATTATACACT ATTGGATGTT
    CTCTACTATG TTACACGTGA TGACTTAAAA TGCTTGAGAC TAAGGGGAGG GATGCTGTGC
    ACACTGTGGA AGGCTATCAT TGACTTTCGA AACAAACAGA CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005914.1
    CDS362..4486
    Translation

    Target ORF information:

    RefSeq Version NM_005923.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005923.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCACGG AGGCGGACGA GGGCATCACT TTCTCTGTGC CACCCTTCGC CCCCTCGGGC 
    TTCTGCACCA TCCCCGAGGG CGGCATCTGC AGGAGGGGAG GAGCGGCGGC GGTGGGCGAG
    GGCGAGGAGC ACCAGCTGCC ACCGCCGCCG CCGGGCAGCT TCTGGAACGT GGAGAGCGCC
    GCTGCCCCTG GCATCGGTTG TCCGGCGGCC ACCTCCTCGA GCAGTGCCAC CCGAGGCCGG
    GGCAGCTCTG TTGGCGGGGG CAGCCGACGG ACCACGGTGG CATATGTGAT CAACGAAGCG
    AGCCAAGGGC AACTGGTGGT GGCCGAGAGC GAGGCCCTGC AGAGCTTGCG GGAGGCGTGC
    GAGACAGTGG GCGCCACCCT GGAAACCCTG CATTTTGGGA AACTCGACTT TGGAGAAACC
    ACCGTGCTGG ACCGCTTTTA CAATGCAGAT ATTGCGGTGG TGGAGATGAG CGATGCCTTC
    CGGCAGCCGT CCTTGTTTTA CCACCTTGGG GTGAGAGAAA GTTTCAGCAT GGCCAACAAC
    ATCATCCTCT ACTGTGATAC TAACTCGGAC TCTCTGCAGT CACTGAAGGA AATAATTTGC
    CAGAAGAATA CTATGTGCAC TGGGAACTAC ACCTTTGTTC CTTACATGAT AACTCCACAT
    AACAAAGTCT ACTGCTGTGA CAGCAGCTTC ATGAAGGGGT TGACAGAGCT CATGCAACCG
    AACTTCGAGC TGCTTCTTGG ACCCATCTGC TTACCTCTTG TGGATCGTTT TATTCAACTT
    TTGAAGGTGG CACAAGCAAG TTCTAGCCAG TACTTCCGGG AATCTATACT CAATGACATC
    AGGAAAGCTC GTAATTTATA CACTGGTAAA GAATTGGCAG CTGAGTTGGC AAGAATTCGG
    CAGCGAGTAG ATAATATCGA AGTCTTGACA GCAGATATTG TCATAAATCT GTTACTTTCC
    TACAGAGATA TCCAGGACTA TGATTCTATT GTGAAGCTGG TAGAGACTTT AGAAAAACTG
    CCAACCTTTG ATTTGGCCTC CCATCACCAT GTGAAGTTTC ATTATGCATT TGCACTGAAT
    AGGAGAAATC TCCCTGGTGA CAGAGCAAAA GCTCTTGATA TTATGATTCC CATGGTGCAA
    AGCGAAGGAC AAGTTGCTTC AGATATGTAT TGCCTAGTTG GTCGAATCTA CAAAGATATG
    TTTTTGGACT CTAATTTCAC GGACACTGAA AGCAGAGACC ATGGAGCTTC TTGGTTCAAA
    AAGGCATTTG AATCTGAGCC AACACTACAG TCAGGAATTA ATTATGCGGT CCTCCTCCTG
    GCAGCTGGAC ACCAGTTTGA ATCTTCCTTT GAGCTCCGGA AAGTTGGGGT GAAGCTAAGT
    AGTCTTCTTG GTAAAAAGGG AAACTTGGAA AAACTCCAGA GCTACTGGGA AGTTGGATTT
    TTTCTGGGGG CCAGCGTCCT AGCCAATGAC CACATGAGAG TCATTCAAGC ATCTGAAAAG
    CTTTTTAAAC TGAAGACACC AGCATGGTAC CTCAAGTCTA TTGTAGAGAC AATTTTAATA
    TATAAGCATT TTGTGAAACT GACCACAGAA CAGCCTGTGG CCAAGCAAGA ACTTGTGGAC
    TTTTGGATGG ATTTCCTGGT CGAGGCCACA AAGACAGATG TTACTGTGGT TAGGTTTCCA
    GTATTAATAT TAGAACCAAC CAAAATCTAT CAACCTTCTT ATTTGTCTAT CAACAATGAA
    GTTGAGGAAA AGACAATCTC TATTTGGCAC GTGCTTCCTG ATGACAAGAA AGGTATACAT
    GAGTGGAATT TTAGTGCCTC TTCTGTCAGG GGAGTGAGTA TTTCTAAATT TGAAGAAAGA
    TGCTGCTTTC TTTATGTGCT TCACAATTCT GATGATTTCC AAATCTATTT CTGTACAGAA
    CTTCATTGTA AAAAGTTTTT TGAGATGGTG AACACCATTA CCGAAGAGAA GGGGAGAAGC
    ACAGAGGAAG GAGACTGTGA AAGTGACTTG CTGGAGTATG ACTATGAATA TGATGAAAAT
    GGTGACAGAG TCGTTTTAGG AAAAGGCACT TATGGGATAG TCTACGCAGG TCGGGACTTG
    AGCAACCAAG TCAGAATTGC TATTAAGGAA ATCCCAGAGA GAGACAGCAG ATACTCTCAG
    CCCCTGCATG AAGAAATAGC ATTGCATAAA CACCTGAAGC ACAAAAATAT TGTCCAGTAT
    CTGGGCTCTT TCAGTGAGAA TGGTTTCATT AAAATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTTTCTG CTCTCCTTCG TTCCAAATGG GGTCCATTAA AAGACAATGA GCAAACAATT
    GGCTTTTATA CAAAGCAAAT ACTGGAAGGA TTAAAATATC TCCATGACAA TCAGATAGTT
    CACCGGGACA TAAAGGGTGA CAATGTGTTG ATTAATACCT ACAGTGGTGT TCTCAAGATC
    TCTGACTTCG GAACATCAAA GAGGCTTGCT GGCATAAACC CCTGTACTGA AACTTTTACT
    GGTACCCTCC AGTATATGGC ACCAGAAATA ATAGATAAAG GACCAAGAGG CTACGGAAAA
    GCAGCAGACA TCTGGTCTCT GGGCTGTACA ATCATTGAAA TGGCCACAGG AAAACCCCCA
    TTTTATGAAC TGGGAGAACC ACAAGCAGCT ATGTTCAAGG TGGGAATGTT TAAAGTCCAC
    CCTGAGATCC CAGAGTCCAT GTCTGCAGAG GCCAAGGCAT TCATACTGAA ATGTTTTGAA
    CCAGATCCTG ACAAGAGAGC CTGTGCTAAC GACTTGCTTG TTGATGAGTT TTTAAAAGTT
    TCAAGCAAAA AGAAAAAGAC ACAACCTAAG CTTTCAGCTC TTTCAGCTGG ATCAAATGAA
    TATCTCAGGA GTATATCCTT GCCGGTACCT GTGCTGGTGG AGGACACCAG CAGCAGCAGT
    GAGTACGGCT CAGTTTCACC CGACACGGAG TTGAAAGTGG ACCCCTTCTC TTTCAAAACA
    AGAGCCAAGT CCTGCGGAGA AAGAGATGTC AAGGGAATTC GGACACTCTT TTTGGGCATT
    CCAGATGAGA ATTTTGAAGA TCACAGTGCT CCTCCTTCCC CTGAAGAAAA AGATTCTGGA
    TTCTTCATGC TGAGGAAGGA CAGTGAGAGG CGAGCTACCC TTCACAGGAT CCTGACGGAA
    GACCAAGACA AAATTGTGAG AAACCTAATG GAATCTTTAG CTCAGGGGGC TGAAGAACCG
    AAACTAAAAT GGGAACACAT CACAACCCTC ATTGCAAGCC TCAGAGAATT TGTGAGATCC
    ACTGACCGAA AAATCATAGC CACCACACTG TCAAAGCTGA AACTGGAGCT GGACTTCGAC
    AGCCATGGCA TTAGCCAAGT CCAGGTGGTA CTCTTTGGTT TTCAAGATGC TGTCAATAAA
    GTTCTTCGGA ATCATAACAT CAAGCCGCAC TGGATGTTTG CCTTAGACAG TATCATTCGG
    AAGGCGGTAC AGACAGCCAT TACCATCCTG GTTCCAGAAC TAAGGCCACA TTTCAGCCTT
    GCATCTGAGA GTGATACTGC TGATCAAGAA GACTTGGATG TAGAAGATGA CCATGAGGAA
    CAGCCTTCAA ATCAAACTGT CCGAAGACCT CAGGCTGTCA TTGAAGATGC TGTGGCTACC
    TCAGGCGTGA GCACGCTCAG TTCTACTGTG TCTCATGATT CCCAGAGTGC TCACCGGTCA
    CTGAATGTAC AGCTTGGAAG GATGAAAATA GAAACCAATA GATTACTGGA AGAATTGGTT
    CGGAAAGAGA AAGAATTACA AGCACTCCTT CATCGAGCTA TTGAAGAAAA AGACCAAGAA
    ATTAAACACC TGAAGCTTAA GTCCCAACCC ATAGAAATTC CTGAATTGCC TGTATTTCAT
    CTAAATTCTT CTGGCACAAA TACTGAAGAT TCTGAACTTA CCGACTGGCT GAGAGTGAAT
    GGAGCTGATG AAGACACTAT AAGCCGGTTT TTGGCTGAAG ATTATACACT ATTGGATGTT
    CTCTACTATG TTACACGTGA TGACTTAAAA TGCTTGAGAC TAAGGGGAGG GATGCTGTGC
    ACACTGTGGA AGGCTATCAT TGACTTTCGA AACAAACAGA CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19594
    Clone ID Related Accession (Same CDS sequence) NM_005923.3 , NM_005923.4
    Accession Version NM_005923.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-02
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121933.15 and AL024508.1. This sequence is a reference standard in the RefSeqGene project. On Nov 26, 2018 this sequence version replaced NM_005923.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.22198.1, U67156.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000359015.5/ ENSP00000351908.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCACGG AGGCGGACGA GGGCATCACT TTCTCTGTGC CACCCTTCGC CCCCTCGGGC 
    TTCTGCACCA TCCCCGAGGG CGGCATCTGC AGGAGGGGAG GAGCGGCGGC GGTGGGCGAG
    GGCGAGGAGC ACCAGCTGCC ACCGCCGCCG CCGGGCAGCT TCTGGAACGT GGAGAGCGCC
    GCTGCCCCTG GCATCGGTTG TCCGGCGGCC ACCTCCTCGA GCAGTGCCAC CCGAGGCCGG
    GGCAGCTCTG TTGGCGGGGG CAGCCGACGG ACCACGGTGG CATATGTGAT CAACGAAGCG
    AGCCAAGGGC AACTGGTGGT GGCCGAGAGC GAGGCCCTGC AGAGCTTGCG GGAGGCGTGC
    GAGACAGTGG GCGCCACCCT GGAAACCCTG CATTTTGGGA AACTCGACTT TGGAGAAACC
    ACCGTGCTGG ACCGCTTTTA CAATGCAGAT ATTGCGGTGG TGGAGATGAG CGATGCCTTC
    CGGCAGCCGT CCTTGTTTTA CCACCTTGGG GTGAGAGAAA GTTTCAGCAT GGCCAACAAC
    ATCATCCTCT ACTGTGATAC TAACTCGGAC TCTCTGCAGT CACTGAAGGA AATAATTTGC
    CAGAAGAATA CTATGTGCAC TGGGAACTAC ACCTTTGTTC CTTACATGAT AACTCCACAT
    AACAAAGTCT ACTGCTGTGA CAGCAGCTTC ATGAAGGGGT TGACAGAGCT CATGCAACCG
    AACTTCGAGC TGCTTCTTGG ACCCATCTGC TTACCTCTTG TGGATCGTTT TATTCAACTT
    TTGAAGGTGG CACAAGCAAG TTCTAGCCAG TACTTCCGGG AATCTATACT CAATGACATC
    AGGAAAGCTC GTAATTTATA CACTGGTAAA GAATTGGCAG CTGAGTTGGC AAGAATTCGG
    CAGCGAGTAG ATAATATCGA AGTCTTGACA GCAGATATTG TCATAAATCT GTTACTTTCC
    TACAGAGATA TCCAGGACTA TGATTCTATT GTGAAGCTGG TAGAGACTTT AGAAAAACTG
    CCAACCTTTG ATTTGGCCTC CCATCACCAT GTGAAGTTTC ATTATGCATT TGCACTGAAT
    AGGAGAAATC TCCCTGGTGA CAGAGCAAAA GCTCTTGATA TTATGATTCC CATGGTGCAA
    AGCGAAGGAC AAGTTGCTTC AGATATGTAT TGCCTAGTTG GTCGAATCTA CAAAGATATG
    TTTTTGGACT CTAATTTCAC GGACACTGAA AGCAGAGACC ATGGAGCTTC TTGGTTCAAA
    AAGGCATTTG AATCTGAGCC AACACTACAG TCAGGAATTA ATTATGCGGT CCTCCTCCTG
    GCAGCTGGAC ACCAGTTTGA ATCTTCCTTT GAGCTCCGGA AAGTTGGGGT GAAGCTAAGT
    AGTCTTCTTG GTAAAAAGGG AAACTTGGAA AAACTCCAGA GCTACTGGGA AGTTGGATTT
    TTTCTGGGGG CCAGCGTCCT AGCCAATGAC CACATGAGAG TCATTCAAGC ATCTGAAAAG
    CTTTTTAAAC TGAAGACACC AGCATGGTAC CTCAAGTCTA TTGTAGAGAC AATTTTAATA
    TATAAGCATT TTGTGAAACT GACCACAGAA CAGCCTGTGG CCAAGCAAGA ACTTGTGGAC
    TTTTGGATGG ATTTCCTGGT CGAGGCCACA AAGACAGATG TTACTGTGGT TAGGTTTCCA
    GTATTAATAT TAGAACCAAC CAAAATCTAT CAACCTTCTT ATTTGTCTAT CAACAATGAA
    GTTGAGGAAA AGACAATCTC TATTTGGCAC GTGCTTCCTG ATGACAAGAA AGGTATACAT
    GAGTGGAATT TTAGTGCCTC TTCTGTCAGG GGAGTGAGTA TTTCTAAATT TGAAGAAAGA
    TGCTGCTTTC TTTATGTGCT TCACAATTCT GATGATTTCC AAATCTATTT CTGTACAGAA
    CTTCATTGTA AAAAGTTTTT TGAGATGGTG AACACCATTA CCGAAGAGAA GGGGAGAAGC
    ACAGAGGAAG GAGACTGTGA AAGTGACTTG CTGGAGTATG ACTATGAATA TGATGAAAAT
    GGTGACAGAG TCGTTTTAGG AAAAGGCACT TATGGGATAG TCTACGCAGG TCGGGACTTG
    AGCAACCAAG TCAGAATTGC TATTAAGGAA ATCCCAGAGA GAGACAGCAG ATACTCTCAG
    CCCCTGCATG AAGAAATAGC ATTGCATAAA CACCTGAAGC ACAAAAATAT TGTCCAGTAT
    CTGGGCTCTT TCAGTGAGAA TGGTTTCATT AAAATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTTTCTG CTCTCCTTCG TTCCAAATGG GGTCCATTAA AAGACAATGA GCAAACAATT
    GGCTTTTATA CAAAGCAAAT ACTGGAAGGA TTAAAATATC TCCATGACAA TCAGATAGTT
    CACCGGGACA TAAAGGGTGA CAATGTGTTG ATTAATACCT ACAGTGGTGT TCTCAAGATC
    TCTGACTTCG GAACATCAAA GAGGCTTGCT GGCATAAACC CCTGTACTGA AACTTTTACT
    GGTACCCTCC AGTATATGGC ACCAGAAATA ATAGATAAAG GACCAAGAGG CTACGGAAAA
    GCAGCAGACA TCTGGTCTCT GGGCTGTACA ATCATTGAAA TGGCCACAGG AAAACCCCCA
    TTTTATGAAC TGGGAGAACC ACAAGCAGCT ATGTTCAAGG TGGGAATGTT TAAAGTCCAC
    CCTGAGATCC CAGAGTCCAT GTCTGCAGAG GCCAAGGCAT TCATACTGAA ATGTTTTGAA
    CCAGATCCTG ACAAGAGAGC CTGTGCTAAC GACTTGCTTG TTGATGAGTT TTTAAAAGTT
    TCAAGCAAAA AGAAAAAGAC ACAACCTAAG CTTTCAGCTC TTTCAGCTGG ATCAAATGAA
    TATCTCAGGA GTATATCCTT GCCGGTACCT GTGCTGGTGG AGGACACCAG CAGCAGCAGT
    GAGTACGGCT CAGTTTCACC CGACACGGAG TTGAAAGTGG ACCCCTTCTC TTTCAAAACA
    AGAGCCAAGT CCTGCGGAGA AAGAGATGTC AAGGGAATTC GGACACTCTT TTTGGGCATT
    CCAGATGAGA ATTTTGAAGA TCACAGTGCT CCTCCTTCCC CTGAAGAAAA AGATTCTGGA
    TTCTTCATGC TGAGGAAGGA CAGTGAGAGG CGAGCTACCC TTCACAGGAT CCTGACGGAA
    GACCAAGACA AAATTGTGAG AAACCTAATG GAATCTTTAG CTCAGGGGGC TGAAGAACCG
    AAACTAAAAT GGGAACACAT CACAACCCTC ATTGCAAGCC TCAGAGAATT TGTGAGATCC
    ACTGACCGAA AAATCATAGC CACCACACTG TCAAAGCTGA AACTGGAGCT GGACTTCGAC
    AGCCATGGCA TTAGCCAAGT CCAGGTGGTA CTCTTTGGTT TTCAAGATGC TGTCAATAAA
    GTTCTTCGGA ATCATAACAT CAAGCCGCAC TGGATGTTTG CCTTAGACAG TATCATTCGG
    AAGGCGGTAC AGACAGCCAT TACCATCCTG GTTCCAGAAC TAAGGCCACA TTTCAGCCTT
    GCATCTGAGA GTGATACTGC TGATCAAGAA GACTTGGATG TAGAAGATGA CCATGAGGAA
    CAGCCTTCAA ATCAAACTGT CCGAAGACCT CAGGCTGTCA TTGAAGATGC TGTGGCTACC
    TCAGGCGTGA GCACGCTCAG TTCTACTGTG TCTCATGATT CCCAGAGTGC TCACCGGTCA
    CTGAATGTAC AGCTTGGAAG GATGAAAATA GAAACCAATA GATTACTGGA AGAATTGGTT
    CGGAAAGAGA AAGAATTACA AGCACTCCTT CATCGAGCTA TTGAAGAAAA AGACCAAGAA
    ATTAAACACC TGAAGCTTAA GTCCCAACCC ATAGAAATTC CTGAATTGCC TGTATTTCAT
    CTAAATTCTT CTGGCACAAA TACTGAAGAT TCTGAACTTA CCGACTGGCT GAGAGTGAAT
    GGAGCTGATG AAGACACTAT AAGCCGGTTT TTGGCTGAAG ATTATACACT ATTGGATGTT
    CTCTACTATG TTACACGTGA TGACTTAAAA TGCTTGAGAC TAAGGGGAGG GATGCTGTGC
    ACACTGTGGA AGGCTATCAT TGACTTTCGA AACAAACAGA CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005914.1
    CDS321..4445
    Translation

    Target ORF information:

    RefSeq Version NM_005923.4
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005923.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCACGG AGGCGGACGA GGGCATCACT TTCTCTGTGC CACCCTTCGC CCCCTCGGGC 
    TTCTGCACCA TCCCCGAGGG CGGCATCTGC AGGAGGGGAG GAGCGGCGGC GGTGGGCGAG
    GGCGAGGAGC ACCAGCTGCC ACCGCCGCCG CCGGGCAGCT TCTGGAACGT GGAGAGCGCC
    GCTGCCCCTG GCATCGGTTG TCCGGCGGCC ACCTCCTCGA GCAGTGCCAC CCGAGGCCGG
    GGCAGCTCTG TTGGCGGGGG CAGCCGACGG ACCACGGTGG CATATGTGAT CAACGAAGCG
    AGCCAAGGGC AACTGGTGGT GGCCGAGAGC GAGGCCCTGC AGAGCTTGCG GGAGGCGTGC
    GAGACAGTGG GCGCCACCCT GGAAACCCTG CATTTTGGGA AACTCGACTT TGGAGAAACC
    ACCGTGCTGG ACCGCTTTTA CAATGCAGAT ATTGCGGTGG TGGAGATGAG CGATGCCTTC
    CGGCAGCCGT CCTTGTTTTA CCACCTTGGG GTGAGAGAAA GTTTCAGCAT GGCCAACAAC
    ATCATCCTCT ACTGTGATAC TAACTCGGAC TCTCTGCAGT CACTGAAGGA AATAATTTGC
    CAGAAGAATA CTATGTGCAC TGGGAACTAC ACCTTTGTTC CTTACATGAT AACTCCACAT
    AACAAAGTCT ACTGCTGTGA CAGCAGCTTC ATGAAGGGGT TGACAGAGCT CATGCAACCG
    AACTTCGAGC TGCTTCTTGG ACCCATCTGC TTACCTCTTG TGGATCGTTT TATTCAACTT
    TTGAAGGTGG CACAAGCAAG TTCTAGCCAG TACTTCCGGG AATCTATACT CAATGACATC
    AGGAAAGCTC GTAATTTATA CACTGGTAAA GAATTGGCAG CTGAGTTGGC AAGAATTCGG
    CAGCGAGTAG ATAATATCGA AGTCTTGACA GCAGATATTG TCATAAATCT GTTACTTTCC
    TACAGAGATA TCCAGGACTA TGATTCTATT GTGAAGCTGG TAGAGACTTT AGAAAAACTG
    CCAACCTTTG ATTTGGCCTC CCATCACCAT GTGAAGTTTC ATTATGCATT TGCACTGAAT
    AGGAGAAATC TCCCTGGTGA CAGAGCAAAA GCTCTTGATA TTATGATTCC CATGGTGCAA
    AGCGAAGGAC AAGTTGCTTC AGATATGTAT TGCCTAGTTG GTCGAATCTA CAAAGATATG
    TTTTTGGACT CTAATTTCAC GGACACTGAA AGCAGAGACC ATGGAGCTTC TTGGTTCAAA
    AAGGCATTTG AATCTGAGCC AACACTACAG TCAGGAATTA ATTATGCGGT CCTCCTCCTG
    GCAGCTGGAC ACCAGTTTGA ATCTTCCTTT GAGCTCCGGA AAGTTGGGGT GAAGCTAAGT
    AGTCTTCTTG GTAAAAAGGG AAACTTGGAA AAACTCCAGA GCTACTGGGA AGTTGGATTT
    TTTCTGGGGG CCAGCGTCCT AGCCAATGAC CACATGAGAG TCATTCAAGC ATCTGAAAAG
    CTTTTTAAAC TGAAGACACC AGCATGGTAC CTCAAGTCTA TTGTAGAGAC AATTTTAATA
    TATAAGCATT TTGTGAAACT GACCACAGAA CAGCCTGTGG CCAAGCAAGA ACTTGTGGAC
    TTTTGGATGG ATTTCCTGGT CGAGGCCACA AAGACAGATG TTACTGTGGT TAGGTTTCCA
    GTATTAATAT TAGAACCAAC CAAAATCTAT CAACCTTCTT ATTTGTCTAT CAACAATGAA
    GTTGAGGAAA AGACAATCTC TATTTGGCAC GTGCTTCCTG ATGACAAGAA AGGTATACAT
    GAGTGGAATT TTAGTGCCTC TTCTGTCAGG GGAGTGAGTA TTTCTAAATT TGAAGAAAGA
    TGCTGCTTTC TTTATGTGCT TCACAATTCT GATGATTTCC AAATCTATTT CTGTACAGAA
    CTTCATTGTA AAAAGTTTTT TGAGATGGTG AACACCATTA CCGAAGAGAA GGGGAGAAGC
    ACAGAGGAAG GAGACTGTGA AAGTGACTTG CTGGAGTATG ACTATGAATA TGATGAAAAT
    GGTGACAGAG TCGTTTTAGG AAAAGGCACT TATGGGATAG TCTACGCAGG TCGGGACTTG
    AGCAACCAAG TCAGAATTGC TATTAAGGAA ATCCCAGAGA GAGACAGCAG ATACTCTCAG
    CCCCTGCATG AAGAAATAGC ATTGCATAAA CACCTGAAGC ACAAAAATAT TGTCCAGTAT
    CTGGGCTCTT TCAGTGAGAA TGGTTTCATT AAAATCTTCA TGGAGCAGGT CCCTGGAGGA
    AGTCTTTCTG CTCTCCTTCG TTCCAAATGG GGTCCATTAA AAGACAATGA GCAAACAATT
    GGCTTTTATA CAAAGCAAAT ACTGGAAGGA TTAAAATATC TCCATGACAA TCAGATAGTT
    CACCGGGACA TAAAGGGTGA CAATGTGTTG ATTAATACCT ACAGTGGTGT TCTCAAGATC
    TCTGACTTCG GAACATCAAA GAGGCTTGCT GGCATAAACC CCTGTACTGA AACTTTTACT
    GGTACCCTCC AGTATATGGC ACCAGAAATA ATAGATAAAG GACCAAGAGG CTACGGAAAA
    GCAGCAGACA TCTGGTCTCT GGGCTGTACA ATCATTGAAA TGGCCACAGG AAAACCCCCA
    TTTTATGAAC TGGGAGAACC ACAAGCAGCT ATGTTCAAGG TGGGAATGTT TAAAGTCCAC
    CCTGAGATCC CAGAGTCCAT GTCTGCAGAG GCCAAGGCAT TCATACTGAA ATGTTTTGAA
    CCAGATCCTG ACAAGAGAGC CTGTGCTAAC GACTTGCTTG TTGATGAGTT TTTAAAAGTT
    TCAAGCAAAA AGAAAAAGAC ACAACCTAAG CTTTCAGCTC TTTCAGCTGG ATCAAATGAA
    TATCTCAGGA GTATATCCTT GCCGGTACCT GTGCTGGTGG AGGACACCAG CAGCAGCAGT
    GAGTACGGCT CAGTTTCACC CGACACGGAG TTGAAAGTGG ACCCCTTCTC TTTCAAAACA
    AGAGCCAAGT CCTGCGGAGA AAGAGATGTC AAGGGAATTC GGACACTCTT TTTGGGCATT
    CCAGATGAGA ATTTTGAAGA TCACAGTGCT CCTCCTTCCC CTGAAGAAAA AGATTCTGGA
    TTCTTCATGC TGAGGAAGGA CAGTGAGAGG CGAGCTACCC TTCACAGGAT CCTGACGGAA
    GACCAAGACA AAATTGTGAG AAACCTAATG GAATCTTTAG CTCAGGGGGC TGAAGAACCG
    AAACTAAAAT GGGAACACAT CACAACCCTC ATTGCAAGCC TCAGAGAATT TGTGAGATCC
    ACTGACCGAA AAATCATAGC CACCACACTG TCAAAGCTGA AACTGGAGCT GGACTTCGAC
    AGCCATGGCA TTAGCCAAGT CCAGGTGGTA CTCTTTGGTT TTCAAGATGC TGTCAATAAA
    GTTCTTCGGA ATCATAACAT CAAGCCGCAC TGGATGTTTG CCTTAGACAG TATCATTCGG
    AAGGCGGTAC AGACAGCCAT TACCATCCTG GTTCCAGAAC TAAGGCCACA TTTCAGCCTT
    GCATCTGAGA GTGATACTGC TGATCAAGAA GACTTGGATG TAGAAGATGA CCATGAGGAA
    CAGCCTTCAA ATCAAACTGT CCGAAGACCT CAGGCTGTCA TTGAAGATGC TGTGGCTACC
    TCAGGCGTGA GCACGCTCAG TTCTACTGTG TCTCATGATT CCCAGAGTGC TCACCGGTCA
    CTGAATGTAC AGCTTGGAAG GATGAAAATA GAAACCAATA GATTACTGGA AGAATTGGTT
    CGGAAAGAGA AAGAATTACA AGCACTCCTT CATCGAGCTA TTGAAGAAAA AGACCAAGAA
    ATTAAACACC TGAAGCTTAA GTCCCAACCC ATAGAAATTC CTGAATTGCC TGTATTTCAT
    CTAAATTCTT CTGGCACAAA TACTGAAGAT TCTGAACTTA CCGACTGGCT GAGAGTGAAT
    GGAGCTGATG AAGACACTAT AAGCCGGTTT TTGGCTGAAG ATTATACACT ATTGGATGTT
    CTCTACTATG TTACACGTGA TGACTTAAAA TGCTTGAGAC TAAGGGGAGG GATGCTGTGC
    ACACTGTGGA AGGCTATCAT TGACTTTCGA AACAAACAGA CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96613
    Clone ID Related Accession (Same CDS sequence) XM_017010876.1
    Accession Version XM_017010876.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3576bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 5 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGGAAA TAATTTGCCA GAAGAATACT ATGTGCACTG GGAACTACAC CTTTGTTCCT 
    TACATGATAA CTCCACATAA CAAAGTCTAC TGCTGTGACA GCAGCTTCAT GAAGGGGTTG
    ACAGAGCTCA TGCAACCGAA CTTCGAGCTG CTTCTTGGAC CCATCTGCTT ACCTCTTGTG
    GATCGTTTTA TTCAACTTTT GAAGGTGGCA CAAGCAAGTT CTAGCCAGTA CTTCCGGGAA
    TCTATACTCA ATGACATCAG GAAAGCTCGT AATTTATACA CTGGTAAAGA ATTGGCAGCT
    GAGTTGGCAA GAATTCGGCA GCGAGTAGAT AATATCGAAG TCTTGACAGC AGATATTGTC
    ATAAATCTGT TACTTTCCTA CAGAGATATC CAGGACTATG ATTCTATTGT GAAGCTGGTA
    GAGACTTTAG AAAAACTGCC AACCTTTGAT TTGGCCTCCC ATCACCATGT GAAGTTTCAT
    TATGCATTTG CACTGAATAG GAGAAATCTC CCTGGTGACA GAGCAAAAGC TCTTGATATT
    ATGATTCCCA TGGTGCAAAG CGAAGGACAA GTTGCTTCAG ATATGTATTG CCTAGTTGGT
    CGAATCTACA AAGATATGTT TTTGGACTCT AATTTCACGG ACACTGAAAG CAGAGACCAT
    GGAGCTTCTT GGTTCAAAAA GGCATTTGAA TCTGAGCCAA CACTACAGTC AGGAATTAAT
    TATGCGGTCC TCCTCCTGGC AGCTGGACAC CAGTTTGAAT CTTCCTTTGA GCTCCGGAAA
    GTTGGGGTGA AGCTAAGTAG TCTTCTTGGT AAAAAGGGAA ACTTGGAAAA ACTCCAGAGC
    TACTGGGAAG TTGGATTTTT TCTGGGGGCC AGCGTCCTAG CCAATGACCA CATGAGAGTC
    ATTCAAGCAT CTGAAAAGCT TTTTAAACTG AAGACACCAG CATGGTACCT CAAGTCTATT
    GTAGAGACAA TTTTAATATA TAAGCATTTT GTGAAACTGA CCACAGAACA GCCTGTGGCC
    AAGCAAGAAC TTGTGGACTT TTGGATGGAT TTCCTGGTCG AGGCCACAAA GACAGATGTT
    ACTGTGGTTA GGTTTCCAGT ATTAATATTA GAACCAACCA AAATCTATCA ACCTTCTTAT
    TTGTCTATCA ACAATGAAGT TGAGGAAAAG ACAATCTCTA TTTGGCACGT GCTTCCTGAT
    GACAAGAAAG GTATACATGA GTGGAATTTT AGTGCCTCTT CTGTCAGGGG AGTGAGTATT
    TCTAAATTTG AAGAAAGATG CTGCTTTCTT TATGTGCTTC ACAATTCTGA TGATTTCCAA
    ATCTATTTCT GTACAGAACT TCATTGTAAA AAGTTTTTTG AGATGGTGAA CACCATTACC
    GAAGAGAAGG GGAGAAGCAC AGAGGAAGGA GACTGTGAAA GTGACTTGCT GGAGTATGAC
    TATGAATATG ATGAAAATGG TGACAGAGTC GTTTTAGGAA AAGGCACTTA TGGGATAGTC
    TACGCAGGTC GGGACTTGAG CAACCAAGTC AGAATTGCTA TTAAGGAAAT CCCAGAGAGA
    GACAGCAGAT ACTCTCAGCC CCTGCATGAA GAAATAGCAT TGCATAAACA CCTGAAGCAC
    AAAAATATTG TCCAGTATCT GGGCTCTTTC AGTGAGAATG GTTTCATTAA AATCTTCATG
    GAGCAGGTCC CTGGAGGAAG TCTTTCTGCT CTCCTTCGTT CCAAATGGGG TCCATTAAAA
    GACAATGAGC AAACAATTGG CTTTTATACA AAGCAAATAC TGGAAGGATT AAAATATCTC
    CATGACAATC AGATAGTTCA CCGGGACATA AAGGGTGACA ATGTGTTGAT TAATACCTAC
    AGTGGTGTTC TCAAGATCTC TGACTTCGGA ACATCAAAGA GGCTTGCTGG CATAAACCCC
    TGTACTGAAA CTTTTACTGG TACCCTCCAG TATATGGCAC CAGAAATAAT AGATAAAGGA
    CCAAGAGGCT ACGGAAAAGC AGCAGACATC TGGTCTCTGG GCTGTACAAT CATTGAAATG
    GCCACAGGAA AACCCCCATT TTATGAACTG GGAGAACCAC AAGCAGCTAT GTTCAAGGTG
    GGAATGTTTA AAGTCCACCC TGAGATCCCA GAGTCCATGT CTGCAGAGGC CAAGGCATTC
    ATACTGAAAT GTTTTGAACC AGATCCTGAC AAGAGAGCCT GTGCTAACGA CTTGCTTGTT
    GATGAGTTTT TAAAAGTTTC AAGCAAAAAG AAAAAGACAC AACCTAAGCT TTCAGCTCTT
    TCAGCTGGAT CAAATGCAGA ATATCTCAGG AGTATATCCT TGCCGGTACC TGTGCTGGTG
    GAGGACACCA GCAGCAGCAG TGAGTACGGC TCAGTTTCAC CCGACACGGA GTTGAAAGTG
    GACCCCTTCT CTTTCAAAAC AAGAGCCAAG TCCTGCGGAG AAAGAGATGT CAAGGGAATT
    CGGACACTCT TTTTGGGCAT TCCAGATGAG AATTTTGAAG ATCACAGTGC TCCTCCTTCC
    CCTGAAGAAA AAGATTCTGG ATTCTTCATG CTGAGGAAGG ACAGTGAGAG GCGAGCTACC
    CTTCACAGGA TCCTGACGGA AGACCAAGAC AAAATTGTGA GAAACCTAAT GGAATCTTTA
    GCTCAGGGGG CTGAAGAACC GAAACTAAAA TGGGAACACA TCACAACCCT CATTGCAAGC
    CTCAGAGAAT TTGTGAGATC CACTGACCGA AAAATCATAG CCACCACACT GTCAAAGCTG
    AAACTGGAGC TGGACTTCGA CAGCCATGGC ATTAGCCAAG TCCAGGTGGT ACTCTTTGGT
    TTTCAAGATG CTGTCAATAA AGTTCTTCGG AATCATAACA TCAAGCCGCA CTGGATGTTT
    GCCTTAGACA GTATCATTCG GAAGGCGGTA CAGACAGCCA TTACCATCCT GGTTCCAGAA
    CTAAGGCCAC ATTTCAGCCT TGCATCTGAG AGTGATACTG CTGATCAAGA AGACTTGGAT
    GTAGAAGATG ACCATGAGGA ACAGCCTTCA AATCAAACTG TCCGAAGACC TCAGGCTGTC
    ATTGAAGATG CTGTGGCTAC CTCAGGCGTG AGCACGCTCA GTTCTACTGT GTCTCATGAT
    TCCCAGAGTG CTCACCGGTC ACTGAATGTA CAGCTTGGAA GGATGAAAAT AGAAACCAAT
    AGATTACTGG AAGAATTGGT TCGGAAAGAG AAAGAATTAC AAGCACTCCT TCATCGAGCT
    ATTGAAGAAA AAGACCAAGA AATTAAACAC CTGAAGCTTA AGTCCCAACC CATAGAAATT
    CCTGAATTGC CTGTATTTCA TCTAAATTCT TCTGGCACAA ATACTGAAGA TTCTGAACTT
    ACCGACTGGC TGAGAGTGAA TGGAGCTGAT GAAGACACTA TAAGCCGGTT TTTGGCTGAA
    GATTATACAC TATTGGATGT TCTCTACTAT GTTACACGTG ATGACTTAAA ATGCTTGAGA
    CTAAGGTACC ACTTTTCTTT CTGTTGTCAC TATGGAAAGA GCACCAGGCA AATATCAAAA
    AGTCTGAGTA TCTTCCCAGG CTGGGTCTCT AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866365.1
    CDS275..3850
    Translation

    Target ORF information:

    RefSeq Version XM_017010876.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010876.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGGAAA TAATTTGCCA GAAGAATACT ATGTGCACTG GGAACTACAC CTTTGTTCCT 
    TACATGATAA CTCCACATAA CAAAGTCTAC TGCTGTGACA GCAGCTTCAT GAAGGGGTTG
    ACAGAGCTCA TGCAACCGAA CTTCGAGCTG CTTCTTGGAC CCATCTGCTT ACCTCTTGTG
    GATCGTTTTA TTCAACTTTT GAAGGTGGCA CAAGCAAGTT CTAGCCAGTA CTTCCGGGAA
    TCTATACTCA ATGACATCAG GAAAGCTCGT AATTTATACA CTGGTAAAGA ATTGGCAGCT
    GAGTTGGCAA GAATTCGGCA GCGAGTAGAT AATATCGAAG TCTTGACAGC AGATATTGTC
    ATAAATCTGT TACTTTCCTA CAGAGATATC CAGGACTATG ATTCTATTGT GAAGCTGGTA
    GAGACTTTAG AAAAACTGCC AACCTTTGAT TTGGCCTCCC ATCACCATGT GAAGTTTCAT
    TATGCATTTG CACTGAATAG GAGAAATCTC CCTGGTGACA GAGCAAAAGC TCTTGATATT
    ATGATTCCCA TGGTGCAAAG CGAAGGACAA GTTGCTTCAG ATATGTATTG CCTAGTTGGT
    CGAATCTACA AAGATATGTT TTTGGACTCT AATTTCACGG ACACTGAAAG CAGAGACCAT
    GGAGCTTCTT GGTTCAAAAA GGCATTTGAA TCTGAGCCAA CACTACAGTC AGGAATTAAT
    TATGCGGTCC TCCTCCTGGC AGCTGGACAC CAGTTTGAAT CTTCCTTTGA GCTCCGGAAA
    GTTGGGGTGA AGCTAAGTAG TCTTCTTGGT AAAAAGGGAA ACTTGGAAAA ACTCCAGAGC
    TACTGGGAAG TTGGATTTTT TCTGGGGGCC AGCGTCCTAG CCAATGACCA CATGAGAGTC
    ATTCAAGCAT CTGAAAAGCT TTTTAAACTG AAGACACCAG CATGGTACCT CAAGTCTATT
    GTAGAGACAA TTTTAATATA TAAGCATTTT GTGAAACTGA CCACAGAACA GCCTGTGGCC
    AAGCAAGAAC TTGTGGACTT TTGGATGGAT TTCCTGGTCG AGGCCACAAA GACAGATGTT
    ACTGTGGTTA GGTTTCCAGT ATTAATATTA GAACCAACCA AAATCTATCA ACCTTCTTAT
    TTGTCTATCA ACAATGAAGT TGAGGAAAAG ACAATCTCTA TTTGGCACGT GCTTCCTGAT
    GACAAGAAAG GTATACATGA GTGGAATTTT AGTGCCTCTT CTGTCAGGGG AGTGAGTATT
    TCTAAATTTG AAGAAAGATG CTGCTTTCTT TATGTGCTTC ACAATTCTGA TGATTTCCAA
    ATCTATTTCT GTACAGAACT TCATTGTAAA AAGTTTTTTG AGATGGTGAA CACCATTACC
    GAAGAGAAGG GGAGAAGCAC AGAGGAAGGA GACTGTGAAA GTGACTTGCT GGAGTATGAC
    TATGAATATG ATGAAAATGG TGACAGAGTC GTTTTAGGAA AAGGCACTTA TGGGATAGTC
    TACGCAGGTC GGGACTTGAG CAACCAAGTC AGAATTGCTA TTAAGGAAAT CCCAGAGAGA
    GACAGCAGAT ACTCTCAGCC CCTGCATGAA GAAATAGCAT TGCATAAACA CCTGAAGCAC
    AAAAATATTG TCCAGTATCT GGGCTCTTTC AGTGAGAATG GTTTCATTAA AATCTTCATG
    GAGCAGGTCC CTGGAGGAAG TCTTTCTGCT CTCCTTCGTT CCAAATGGGG TCCATTAAAA
    GACAATGAGC AAACAATTGG CTTTTATACA AAGCAAATAC TGGAAGGATT AAAATATCTC
    CATGACAATC AGATAGTTCA CCGGGACATA AAGGGTGACA ATGTGTTGAT TAATACCTAC
    AGTGGTGTTC TCAAGATCTC TGACTTCGGA ACATCAAAGA GGCTTGCTGG CATAAACCCC
    TGTACTGAAA CTTTTACTGG TACCCTCCAG TATATGGCAC CAGAAATAAT AGATAAAGGA
    CCAAGAGGCT ACGGAAAAGC AGCAGACATC TGGTCTCTGG GCTGTACAAT CATTGAAATG
    GCCACAGGAA AACCCCCATT TTATGAACTG GGAGAACCAC AAGCAGCTAT GTTCAAGGTG
    GGAATGTTTA AAGTCCACCC TGAGATCCCA GAGTCCATGT CTGCAGAGGC CAAGGCATTC
    ATACTGAAAT GTTTTGAACC AGATCCTGAC AAGAGAGCCT GTGCTAACGA CTTGCTTGTT
    GATGAGTTTT TAAAAGTTTC AAGCAAAAAG AAAAAGACAC AACCTAAGCT TTCAGCTCTT
    TCAGCTGGAT CAAATGCAGA ATATCTCAGG AGTATATCCT TGCCGGTACC TGTGCTGGTG
    GAGGACACCA GCAGCAGCAG TGAGTACGGC TCAGTTTCAC CCGACACGGA GTTGAAAGTG
    GACCCCTTCT CTTTCAAAAC AAGAGCCAAG TCCTGCGGAG AAAGAGATGT CAAGGGAATT
    CGGACACTCT TTTTGGGCAT TCCAGATGAG AATTTTGAAG ATCACAGTGC TCCTCCTTCC
    CCTGAAGAAA AAGATTCTGG ATTCTTCATG CTGAGGAAGG ACAGTGAGAG GCGAGCTACC
    CTTCACAGGA TCCTGACGGA AGACCAAGAC AAAATTGTGA GAAACCTAAT GGAATCTTTA
    GCTCAGGGGG CTGAAGAACC GAAACTAAAA TGGGAACACA TCACAACCCT CATTGCAAGC
    CTCAGAGAAT TTGTGAGATC CACTGACCGA AAAATCATAG CCACCACACT GTCAAAGCTG
    AAACTGGAGC TGGACTTCGA CAGCCATGGC ATTAGCCAAG TCCAGGTGGT ACTCTTTGGT
    TTTCAAGATG CTGTCAATAA AGTTCTTCGG AATCATAACA TCAAGCCGCA CTGGATGTTT
    GCCTTAGACA GTATCATTCG GAAGGCGGTA CAGACAGCCA TTACCATCCT GGTTCCAGAA
    CTAAGGCCAC ATTTCAGCCT TGCATCTGAG AGTGATACTG CTGATCAAGA AGACTTGGAT
    GTAGAAGATG ACCATGAGGA ACAGCCTTCA AATCAAACTG TCCGAAGACC TCAGGCTGTC
    ATTGAAGATG CTGTGGCTAC CTCAGGCGTG AGCACGCTCA GTTCTACTGT GTCTCATGAT
    TCCCAGAGTG CTCACCGGTC ACTGAATGTA CAGCTTGGAA GGATGAAAAT AGAAACCAAT
    AGATTACTGG AAGAATTGGT TCGGAAAGAG AAAGAATTAC AAGCACTCCT TCATCGAGCT
    ATTGAAGAAA AAGACCAAGA AATTAAACAC CTGAAGCTTA AGTCCCAACC CATAGAAATT
    CCTGAATTGC CTGTATTTCA TCTAAATTCT TCTGGCACAA ATACTGAAGA TTCTGAACTT
    ACCGACTGGC TGAGAGTGAA TGGAGCTGAT GAAGACACTA TAAGCCGGTT TTTGGCTGAA
    GATTATACAC TATTGGATGT TCTCTACTAT GTTACACGTG ATGACTTAAA ATGCTTGAGA
    CTAAGGTACC ACTTTTCTTT CTGTTGTCAC TATGGAAAGA GCACCAGGCA AATATCAAAA
    AGTCTGAGTA TCTTCCCAGG CTGGGTCTCT AATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96614
    Clone ID Related Accession (Same CDS sequence) XM_017010877.1
    Accession Version XM_017010877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 5 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGGAAA TAATTTGCCA GAAGAATACT ATGTGCACTG GGAACTACAC CTTTGTTCCT 
    TACATGATAA CTCCACATAA CAAAGTCTAC TGCTGTGACA GCAGCTTCAT GAAGGGGTTG
    ACAGAGCTCA TGCAACCGAA CTTCGAGCTG CTTCTTGGAC CCATCTGCTT ACCTCTTGTG
    GATCGTTTTA TTCAACTTTT GAAGGTGGCA CAAGCAAGTT CTAGCCAGTA CTTCCGGGAA
    TCTATACTCA ATGACATCAG GAAAGCTCGT AATTTATACA CTGGTAAAGA ATTGGCAGCT
    GAGTTGGCAA GAATTCGGCA GCGAGTAGAT AATATCGAAG TCTTGACAGC AGATATTGTC
    ATAAATCTGT TACTTTCCTA CAGAGATATC CAGGACTATG ATTCTATTGT GAAGCTGGTA
    GAGACTTTAG AAAAACTGCC AACCTTTGAT TTGGCCTCCC ATCACCATGT GAAGTTTCAT
    TATGCATTTG CACTGAATAG GAGAAATCTC CCTGGTGACA GAGCAAAAGC TCTTGATATT
    ATGATTCCCA TGGTGCAAAG CGAAGGACAA GTTGCTTCAG ATATGTATTG CCTAGTTGGT
    CGAATCTACA AAGATATGTT TTTGGACTCT AATTTCACGG ACACTGAAAG CAGAGACCAT
    GGAGCTTCTT GGTTCAAAAA GGCATTTGAA TCTGAGCCAA CACTACAGTC AGGAATTAAT
    TATGCGGTCC TCCTCCTGGC AGCTGGACAC CAGTTTGAAT CTTCCTTTGA GCTCCGGAAA
    GTTGGGGTGA AGCTAAGTAG TCTTCTTGGT AAAAAGGGAA ACTTGGAAAA ACTCCAGAGC
    TACTGGGAAG TTGGATTTTT TCTGGGGGCC AGCGTCCTAG CCAATGACCA CATGAGAGTC
    ATTCAAGCAT CTGAAAAGCT TTTTAAACTG AAGACACCAG CATGGTACCT CAAGTCTATT
    GTAGAGACAA TTTTAATATA TAAGCATTTT GTGAAACTGA CCACAGAACA GCCTGTGGCC
    AAGCAAGAAC TTGTGGACTT TTGGATGGAT TTCCTGGTCG AGGCCACAAA GACAGATGTT
    ACTGTGGTTA GGTTTCCAGT ATTAATATTA GAACCAACCA AAATCTATCA ACCTTCTTAT
    TTGTCTATCA ACAATGAAGT TGAGGAAAAG ACAATCTCTA TTTGGCACGT GCTTCCTGAT
    GACAAGAAAG GTATACATGA GTGGAATTTT AGTGCCTCTT CTGTCAGGGG AGTGAGTATT
    TCTAAATTTG AAGAAAGATG CTGCTTTCTT TATGTGCTTC ACAATTCTGA TGATTTCCAA
    ATCTATTTCT GTACAGAACT TCATTGTAAA AAGTTTTTTG AGATGGTGAA CACCATTACC
    GAAGAGAAGG GGAGAAGCAC AGAGGAAGGA GACTGTGAAA GTGACTTGCT GGAGTATGAC
    TATGAATATG ATGAAAATGG TGACAGAGTC GTTTTAGGAA AAGGCACTTA TGGGATAGTC
    TACGCAGGTC GGGACTTGAG CAACCAAGTC AGAATTGCTA TTAAGGAAAT CCCAGAGAGA
    GACAGCAGAT ACTCTCAGCC CCTGCATGAA GAAATAGCAT TGCATAAACA CCTGAAGCAC
    AAAAATATTG TCCAGTATCT GGGCTCTTTC AGTGAGAATG GTTTCATTAA AATCTTCATG
    GAGCAGGTCC CTGGAGGAAG TCTTTCTGCT CTCCTTCGTT CCAAATGGGG TCCATTAAAA
    GACAATGAGC AAACAATTGG CTTTTATACA AAGCAAATAC TGGAAGGATT AAAATATCTC
    CATGACAATC AGATAGTTCA CCGGGACATA AAGGGTGACA ATGTGTTGAT TAATACCTAC
    AGTGGTGTTC TCAAGATCTC TGACTTCGGA ACATCAAAGA GGCTTGCTGG CATAAACCCC
    TGTACTGAAA CTTTTACTGG TACCCTCCAG TATATGGCAC CAGAAATAAT AGATAAAGGA
    CCAAGAGGCT ACGGAAAAGC AGCAGACATC TGGTCTCTGG GCTGTACAAT CATTGAAATG
    GCCACAGGAA AACCCCCATT TTATGAACTG GGAGAACCAC AAGCAGCTAT GTTCAAGGTG
    GGAATGTTTA AAGTCCACCC TGAGATCCCA GAGTCCATGT CTGCAGAGGC CAAGGCATTC
    ATACTGAAAT GTTTTGAACC AGATCCTGAC AAGAGAGCCT GTGCTAACGA CTTGCTTGTT
    GATGAGTTTT TAAAAGTTTC AAGCAAAAAG AAAAAGACAC AACCTAAGCT TTCAGCTCTT
    TCAGCTGGAT CAAATGCAGA ATATCTCAGG AGTATATCCT TGCCGGTACC TGTGCTGGTG
    GAGGACACCA GCAGCAGCAG TGAGTACGGC TCAGTTTCAC CCGACACGGA GTTGAAAGTG
    GACCCCTTCT CTTTCAAAAC AAGAGCCAAG TCCTGCGGAG AAAGAGATGT CAAGGGAATT
    CGGACACTCT TTTTGGGCAT TCCAGATGAG AATTTTGAAG ATCACAGTGC TCCTCCTTCC
    CCTGAAGAAA AAGATTCTGG ATTCTTCATG CTGAGGAAGG ACAGTGAGAG GCGAGCTACC
    CTTCACAGGA TCCTGACGGA AGACCAAGAC AAAATTGTGA GAAACCTAAT GGAATCTTTA
    GCTCAGGGGG CTGAAGAACC GAAACTAAAA TGGGAACACA TCACAACCCT CATTGCAAGC
    CTCAGAGAAT TTGTGAGATC CACTGACCGA AAAATCATAG CCACCACACT GTCAAAGCTG
    AAACTGGAGC TGGACTTCGA CAGCCATGGC ATTAGCCAAG TCCAGGTGGT ACTCTTTGGT
    TTTCAAGATG CTGTCAATAA AGTTCTTCGG AATCATAACA TCAAGCCGCA CTGGATGTTT
    GCCTTAGACA GTATCATTCG GAAGGCGGTA CAGACAGCCA TTACCATCCT GGTTCCAGAA
    CTAAGGCCAC ATTTCAGCCT TGCATCTGAG AGTGATACTG CTGATCAAGA AGACTTGGAT
    GTAGAAGATG ACCATGAGGA ACAGCCTTCA AATCAAACTG TCCGAAGACC TCAGGCTGTC
    ATTGAAGATG CTGTGGCTAC CTCAGGCGTG AGCACGCTCA GTTCTACTGT GTCTCATGAT
    TCCCAGAGTG CTCACCGGTC ACTGAATGTA CAGCTTGGAA GGATGAAAAT AGAAACCAAT
    AGATTACTGG AAGAATTGGT TCGGAAAGAG AAAGAATTAC AAGCACTCCT TCATCGAGCT
    ATTGAAGAAA AAGACCAAGA AATTAAACAC CTGAAGCTTA AGTCCCAACC CATAGAAATT
    CCTGAATTGC CTGTATTTCA TCTAAATTCT TCTGGCACAA ATACTGAAGA TTCTGAACTT
    ACCGACTGGC TGAGAGTGAA TGGAGCTGAT GAAGACACTA TAAGCCGGTT TTTGGCTGAA
    GATTATACAC TATTGGATGT TCTCTACTAT GTTACACGTG ATGACTTAAA ATGCTTGAGA
    CTAAGGGGAG GGATGCTGTG CACACTGTGG AAGGCTATCA TTGACTTTCG AAACAAACAG
    ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866366.1
    CDS512..4057
    Translation

    Target ORF information:

    RefSeq Version XM_017010877.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCAGGAAA TAATTTGCCA GAAGAATACT ATGTGCACTG GGAACTACAC CTTTGTTCCT 
    TACATGATAA CTCCACATAA CAAAGTCTAC TGCTGTGACA GCAGCTTCAT GAAGGGGTTG
    ACAGAGCTCA TGCAACCGAA CTTCGAGCTG CTTCTTGGAC CCATCTGCTT ACCTCTTGTG
    GATCGTTTTA TTCAACTTTT GAAGGTGGCA CAAGCAAGTT CTAGCCAGTA CTTCCGGGAA
    TCTATACTCA ATGACATCAG GAAAGCTCGT AATTTATACA CTGGTAAAGA ATTGGCAGCT
    GAGTTGGCAA GAATTCGGCA GCGAGTAGAT AATATCGAAG TCTTGACAGC AGATATTGTC
    ATAAATCTGT TACTTTCCTA CAGAGATATC CAGGACTATG ATTCTATTGT GAAGCTGGTA
    GAGACTTTAG AAAAACTGCC AACCTTTGAT TTGGCCTCCC ATCACCATGT GAAGTTTCAT
    TATGCATTTG CACTGAATAG GAGAAATCTC CCTGGTGACA GAGCAAAAGC TCTTGATATT
    ATGATTCCCA TGGTGCAAAG CGAAGGACAA GTTGCTTCAG ATATGTATTG CCTAGTTGGT
    CGAATCTACA AAGATATGTT TTTGGACTCT AATTTCACGG ACACTGAAAG CAGAGACCAT
    GGAGCTTCTT GGTTCAAAAA GGCATTTGAA TCTGAGCCAA CACTACAGTC AGGAATTAAT
    TATGCGGTCC TCCTCCTGGC AGCTGGACAC CAGTTTGAAT CTTCCTTTGA GCTCCGGAAA
    GTTGGGGTGA AGCTAAGTAG TCTTCTTGGT AAAAAGGGAA ACTTGGAAAA ACTCCAGAGC
    TACTGGGAAG TTGGATTTTT TCTGGGGGCC AGCGTCCTAG CCAATGACCA CATGAGAGTC
    ATTCAAGCAT CTGAAAAGCT TTTTAAACTG AAGACACCAG CATGGTACCT CAAGTCTATT
    GTAGAGACAA TTTTAATATA TAAGCATTTT GTGAAACTGA CCACAGAACA GCCTGTGGCC
    AAGCAAGAAC TTGTGGACTT TTGGATGGAT TTCCTGGTCG AGGCCACAAA GACAGATGTT
    ACTGTGGTTA GGTTTCCAGT ATTAATATTA GAACCAACCA AAATCTATCA ACCTTCTTAT
    TTGTCTATCA ACAATGAAGT TGAGGAAAAG ACAATCTCTA TTTGGCACGT GCTTCCTGAT
    GACAAGAAAG GTATACATGA GTGGAATTTT AGTGCCTCTT CTGTCAGGGG AGTGAGTATT
    TCTAAATTTG AAGAAAGATG CTGCTTTCTT TATGTGCTTC ACAATTCTGA TGATTTCCAA
    ATCTATTTCT GTACAGAACT TCATTGTAAA AAGTTTTTTG AGATGGTGAA CACCATTACC
    GAAGAGAAGG GGAGAAGCAC AGAGGAAGGA GACTGTGAAA GTGACTTGCT GGAGTATGAC
    TATGAATATG ATGAAAATGG TGACAGAGTC GTTTTAGGAA AAGGCACTTA TGGGATAGTC
    TACGCAGGTC GGGACTTGAG CAACCAAGTC AGAATTGCTA TTAAGGAAAT CCCAGAGAGA
    GACAGCAGAT ACTCTCAGCC CCTGCATGAA GAAATAGCAT TGCATAAACA CCTGAAGCAC
    AAAAATATTG TCCAGTATCT GGGCTCTTTC AGTGAGAATG GTTTCATTAA AATCTTCATG
    GAGCAGGTCC CTGGAGGAAG TCTTTCTGCT CTCCTTCGTT CCAAATGGGG TCCATTAAAA
    GACAATGAGC AAACAATTGG CTTTTATACA AAGCAAATAC TGGAAGGATT AAAATATCTC
    CATGACAATC AGATAGTTCA CCGGGACATA AAGGGTGACA ATGTGTTGAT TAATACCTAC
    AGTGGTGTTC TCAAGATCTC TGACTTCGGA ACATCAAAGA GGCTTGCTGG CATAAACCCC
    TGTACTGAAA CTTTTACTGG TACCCTCCAG TATATGGCAC CAGAAATAAT AGATAAAGGA
    CCAAGAGGCT ACGGAAAAGC AGCAGACATC TGGTCTCTGG GCTGTACAAT CATTGAAATG
    GCCACAGGAA AACCCCCATT TTATGAACTG GGAGAACCAC AAGCAGCTAT GTTCAAGGTG
    GGAATGTTTA AAGTCCACCC TGAGATCCCA GAGTCCATGT CTGCAGAGGC CAAGGCATTC
    ATACTGAAAT GTTTTGAACC AGATCCTGAC AAGAGAGCCT GTGCTAACGA CTTGCTTGTT
    GATGAGTTTT TAAAAGTTTC AAGCAAAAAG AAAAAGACAC AACCTAAGCT TTCAGCTCTT
    TCAGCTGGAT CAAATGCAGA ATATCTCAGG AGTATATCCT TGCCGGTACC TGTGCTGGTG
    GAGGACACCA GCAGCAGCAG TGAGTACGGC TCAGTTTCAC CCGACACGGA GTTGAAAGTG
    GACCCCTTCT CTTTCAAAAC AAGAGCCAAG TCCTGCGGAG AAAGAGATGT CAAGGGAATT
    CGGACACTCT TTTTGGGCAT TCCAGATGAG AATTTTGAAG ATCACAGTGC TCCTCCTTCC
    CCTGAAGAAA AAGATTCTGG ATTCTTCATG CTGAGGAAGG ACAGTGAGAG GCGAGCTACC
    CTTCACAGGA TCCTGACGGA AGACCAAGAC AAAATTGTGA GAAACCTAAT GGAATCTTTA
    GCTCAGGGGG CTGAAGAACC GAAACTAAAA TGGGAACACA TCACAACCCT CATTGCAAGC
    CTCAGAGAAT TTGTGAGATC CACTGACCGA AAAATCATAG CCACCACACT GTCAAAGCTG
    AAACTGGAGC TGGACTTCGA CAGCCATGGC ATTAGCCAAG TCCAGGTGGT ACTCTTTGGT
    TTTCAAGATG CTGTCAATAA AGTTCTTCGG AATCATAACA TCAAGCCGCA CTGGATGTTT
    GCCTTAGACA GTATCATTCG GAAGGCGGTA CAGACAGCCA TTACCATCCT GGTTCCAGAA
    CTAAGGCCAC ATTTCAGCCT TGCATCTGAG AGTGATACTG CTGATCAAGA AGACTTGGAT
    GTAGAAGATG ACCATGAGGA ACAGCCTTCA AATCAAACTG TCCGAAGACC TCAGGCTGTC
    ATTGAAGATG CTGTGGCTAC CTCAGGCGTG AGCACGCTCA GTTCTACTGT GTCTCATGAT
    TCCCAGAGTG CTCACCGGTC ACTGAATGTA CAGCTTGGAA GGATGAAAAT AGAAACCAAT
    AGATTACTGG AAGAATTGGT TCGGAAAGAG AAAGAATTAC AAGCACTCCT TCATCGAGCT
    ATTGAAGAAA AAGACCAAGA AATTAAACAC CTGAAGCTTA AGTCCCAACC CATAGAAATT
    CCTGAATTGC CTGTATTTCA TCTAAATTCT TCTGGCACAA ATACTGAAGA TTCTGAACTT
    ACCGACTGGC TGAGAGTGAA TGGAGCTGAT GAAGACACTA TAAGCCGGTT TTTGGCTGAA
    GATTATACAC TATTGGATGT TCTCTACTAT GTTACACGTG ATGACTTAAA ATGCTTGAGA
    CTAAGGGGAG GGATGCTGTG CACACTGTGG AAGGCTATCA TTGACTTTCG AAACAAACAG
    ACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96606
    Clone ID Related Accession (Same CDS sequence) XM_017010870.1
    Accession Version XM_017010870.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA
    CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG
    CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA
    GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT
    GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC
    ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG
    GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT
    GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC
    CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC
    CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA
    ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT
    CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA
    AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT
    AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC
    ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA
    GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC
    ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG
    TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC
    AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC
    TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT
    TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT
    GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG
    TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC
    AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT
    CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG
    CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG
    GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC
    ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG
    TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA
    CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC
    TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG
    GTTCCAGAAC TAAGGCCACA TTTCAGCCTT GCATCTGAGA GTGATACTGC TGATCAAGAA
    GACTTGGATG TAGAAGATGA CCATGAGGAA CAGCCTTCAA ATCAAACTGT CCGAAGACCT
    CAGGCTGTCA TTGAAGATGC TGTGGCTACC TCAGGCGTGA GCACGCTCAG TTCTACTGTG
    TCTCATGATT CCCAGAGTGC TCACCGGTCA CTGAATGTAC AGCTTGGAAG GATGAAAATA
    GAAACCAATA GATTACTGGA AGAATTGGTT CGGAAAGAGA AAGAATTACA AGCACTCCTT
    CATCGAGCTA TTGAAGAAAA AGACCAAGAA ATTAAACACC TGAAGCTTAA GTCCCAACCC
    ATAGAAATTC CTGAATTGCC TGTATTTCAT CTAAATTCTT CTGGCACAAA TACTGAAGAT
    TCTGAACTTA CCGACTGGCT GAGAGTGAAT GGAGCTGATG AAGACACTAT AAGCCGGTTT
    TTGGCTGAAG ATTATACACT ATTGGATGTT CTCTACTATG TTACACGTGA TGACTTAAAA
    TGCTTGAGAC TAAGGTACCA CTTTTCTTTC TGTTGTCACT ATGGAAAGAG CACCAGGCAA
    ATATCAAAAA GTCTGAGTAT CTTCCCAGGC TGGGTCTCTA ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866359.1
    CDS358..4842
    Translation

    Target ORF information:

    RefSeq Version XM_017010870.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010870.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA
    CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG
    CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA
    GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT
    GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC
    ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG
    GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT
    GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC
    CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC
    CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA
    ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT
    CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA
    AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT
    AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC
    ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA
    GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC
    ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG
    TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC
    AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC
    TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT
    TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT
    GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG
    TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC
    AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT
    CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG
    CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG
    GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC
    ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG
    TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA
    CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC
    TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG
    GTTCCAGAAC TAAGGCCACA TTTCAGCCTT GCATCTGAGA GTGATACTGC TGATCAAGAA
    GACTTGGATG TAGAAGATGA CCATGAGGAA CAGCCTTCAA ATCAAACTGT CCGAAGACCT
    CAGGCTGTCA TTGAAGATGC TGTGGCTACC TCAGGCGTGA GCACGCTCAG TTCTACTGTG
    TCTCATGATT CCCAGAGTGC TCACCGGTCA CTGAATGTAC AGCTTGGAAG GATGAAAATA
    GAAACCAATA GATTACTGGA AGAATTGGTT CGGAAAGAGA AAGAATTACA AGCACTCCTT
    CATCGAGCTA TTGAAGAAAA AGACCAAGAA ATTAAACACC TGAAGCTTAA GTCCCAACCC
    ATAGAAATTC CTGAATTGCC TGTATTTCAT CTAAATTCTT CTGGCACAAA TACTGAAGAT
    TCTGAACTTA CCGACTGGCT GAGAGTGAAT GGAGCTGATG AAGACACTAT AAGCCGGTTT
    TTGGCTGAAG ATTATACACT ATTGGATGTT CTCTACTATG TTACACGTGA TGACTTAAAA
    TGCTTGAGAC TAAGGTACCA CTTTTCTTTC TGTTGTCACT ATGGAAAGAG CACCAGGCAA
    ATATCAAAAA GTCTGAGTAT CTTCCCAGGC TGGGTCTCTA ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96607
    Clone ID Related Accession (Same CDS sequence) XM_017010871.1
    Accession Version XM_017010871.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA
    CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG
    CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA
    GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT
    GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC
    ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG
    GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT
    GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC
    CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC
    CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA
    ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT
    CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA
    AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT
    AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC
    ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA
    GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC
    ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG
    TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC
    AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC
    TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT
    TCAGCTCTTT CAGCTGGATC AAATGAATAT CTCAGGAGTA TATCCTTGCC GGTACCTGTG
    CTGGTGGAGG ACACCAGCAG CAGCAGTGAG TACGGCTCAG TTTCACCCGA CACGGAGTTG
    AAAGTGGACC CCTTCTCTTT CAAAACAAGA GCCAAGTCCT GCGGAGAAAG AGATGTCAAG
    GGAATTCGGA CACTCTTTTT GGGCATTCCA GATGAGAATT TTGAAGATCA CAGTGCTCCT
    CCTTCCCCTG AAGAAAAAGA TTCTGGATTC TTCATGCTGA GGAAGGACAG TGAGAGGCGA
    GCTACCCTTC ACAGGATCCT GACGGAAGAC CAAGACAAAA TTGTGAGAAA CCTAATGGAA
    TCTTTAGCTC AGGGGGCTGA AGAACCGAAA CTAAAATGGG AACACATCAC AACCCTCATT
    GCAAGCCTCA GAGAATTTGT GAGATCCACT GACCGAAAAA TCATAGCCAC CACACTGTCA
    AAGCTGAAAC TGGAGCTGGA CTTCGACAGC CATGGCATTA GCCAAGTCCA GGTGGTACTC
    TTTGGTTTTC AAGATGCTGT CAATAAAGTT CTTCGGAATC ATAACATCAA GCCGCACTGG
    ATGTTTGCCT TAGACAGTAT CATTCGGAAG GCGGTACAGA CAGCCATTAC CATCCTGGTT
    CCAGAACTAA GGCCACATTT CAGCCTTGCA TCTGAGAGTG ATACTGCTGA TCAAGAAGAC
    TTGGATGTAG AAGATGACCA TGAGGAACAG CCTTCAAATC AAACTGTCCG AAGACCTCAG
    GCTGTCATTG AAGATGCTGT GGCTACCTCA GGCGTGAGCA CGCTCAGTTC TACTGTGTCT
    CATGATTCCC AGAGTGCTCA CCGGTCACTG AATGTACAGC TTGGAAGGAT GAAAATAGAA
    ACCAATAGAT TACTGGAAGA ATTGGTTCGG AAAGAGAAAG AATTACAAGC ACTCCTTCAT
    CGAGCTATTG AAGAAAAAGA CCAAGAAATT AAACACCTGA AGCTTAAGTC CCAACCCATA
    GAAATTCCTG AATTGCCTGT ATTTCATCTA AATTCTTCTG GCACAAATAC TGAAGATTCT
    GAACTTACCG ACTGGCTGAG AGTGAATGGA GCTGATGAAG ACACTATAAG CCGGTTTTTG
    GCTGAAGATT ATACACTATT GGATGTTCTC TACTATGTTA CACGTGATGA CTTAAAATGC
    TTGAGACTAA GGTACCACTT TTCTTTCTGT TGTCACTATG GAAAGAGCAC CAGGCAAATA
    TCAAAAAGTC TGAGTATCTT CCCAGGCTGG GTCTCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866360.1
    CDS358..4839
    Translation

    Target ORF information:

    RefSeq Version XM_017010871.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010871.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA
    CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG
    CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA
    GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT
    GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC
    ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG
    GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT
    GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC
    CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC
    CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA
    ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT
    CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA
    AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT
    AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC
    ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA
    GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC
    ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG
    TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC
    AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC
    TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT
    TCAGCTCTTT CAGCTGGATC AAATGAATAT CTCAGGAGTA TATCCTTGCC GGTACCTGTG
    CTGGTGGAGG ACACCAGCAG CAGCAGTGAG TACGGCTCAG TTTCACCCGA CACGGAGTTG
    AAAGTGGACC CCTTCTCTTT CAAAACAAGA GCCAAGTCCT GCGGAGAAAG AGATGTCAAG
    GGAATTCGGA CACTCTTTTT GGGCATTCCA GATGAGAATT TTGAAGATCA CAGTGCTCCT
    CCTTCCCCTG AAGAAAAAGA TTCTGGATTC TTCATGCTGA GGAAGGACAG TGAGAGGCGA
    GCTACCCTTC ACAGGATCCT GACGGAAGAC CAAGACAAAA TTGTGAGAAA CCTAATGGAA
    TCTTTAGCTC AGGGGGCTGA AGAACCGAAA CTAAAATGGG AACACATCAC AACCCTCATT
    GCAAGCCTCA GAGAATTTGT GAGATCCACT GACCGAAAAA TCATAGCCAC CACACTGTCA
    AAGCTGAAAC TGGAGCTGGA CTTCGACAGC CATGGCATTA GCCAAGTCCA GGTGGTACTC
    TTTGGTTTTC AAGATGCTGT CAATAAAGTT CTTCGGAATC ATAACATCAA GCCGCACTGG
    ATGTTTGCCT TAGACAGTAT CATTCGGAAG GCGGTACAGA CAGCCATTAC CATCCTGGTT
    CCAGAACTAA GGCCACATTT CAGCCTTGCA TCTGAGAGTG ATACTGCTGA TCAAGAAGAC
    TTGGATGTAG AAGATGACCA TGAGGAACAG CCTTCAAATC AAACTGTCCG AAGACCTCAG
    GCTGTCATTG AAGATGCTGT GGCTACCTCA GGCGTGAGCA CGCTCAGTTC TACTGTGTCT
    CATGATTCCC AGAGTGCTCA CCGGTCACTG AATGTACAGC TTGGAAGGAT GAAAATAGAA
    ACCAATAGAT TACTGGAAGA ATTGGTTCGG AAAGAGAAAG AATTACAAGC ACTCCTTCAT
    CGAGCTATTG AAGAAAAAGA CCAAGAAATT AAACACCTGA AGCTTAAGTC CCAACCCATA
    GAAATTCCTG AATTGCCTGT ATTTCATCTA AATTCTTCTG GCACAAATAC TGAAGATTCT
    GAACTTACCG ACTGGCTGAG AGTGAATGGA GCTGATGAAG ACACTATAAG CCGGTTTTTG
    GCTGAAGATT ATACACTATT GGATGTTCTC TACTATGTTA CACGTGATGA CTTAAAATGC
    TTGAGACTAA GGTACCACTT TTCTTTCTGT TGTCACTATG GAAAGAGCAC CAGGCAAATA
    TCAAAAAGTC TGAGTATCTT CCCAGGCTGG GTCTCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96608
    Clone ID Related Accession (Same CDS sequence) XM_017010872.1
    Accession Version XM_017010872.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA
    CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG
    CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA
    GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT
    GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC
    ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG
    GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT
    GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC
    CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC
    CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA
    ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT
    CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA
    AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT
    AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC
    ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA
    GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC
    ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG
    TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC
    AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC
    TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT
    TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT
    GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG
    TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC
    AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT
    CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG
    CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG
    GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC
    ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG
    TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA
    CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC
    TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG
    GTTCCAGAAC TAAGGCCACA TTTCAGCCTT GCATCTGAGA GTGATACTGC TGATCAAGAA
    GACTTGGATG TAGAAGATGA CCATGAGGAA CAGCCTTCAA ATCAAACTGT CCGAAGACCT
    CAGGCTGTCA TTGAAGATGC TGTGGCTACC TCAGGCGTGA GCACGCTCAG TTCTACTGTG
    TCTCATGATT CCCAGAGTGC TCACCGGTCA CTGAATGTAC AGCTTGGAAG GATGAAAATA
    GAAACCAATA GATTACTGGA AGAATTGGTT CGGAAAGAGA AAGAATTACA AGCACTCCTT
    CATCGAGCTA TTGAAGAAAA AGACCAAGAA ATTAAACACC TGAAGCTTAA GTCCCAACCC
    ATAGAAATTC CTGAATTGCC TGTATTTCAT CTAAATTCTT CTGGCACAAA TACTGAAGAT
    TCTGAACTTA CCGACTGGCT GAGAGTGAAT GGAGCTGATG AAGACACTAT AAGCCGGTTT
    TTGGCTGAAG ATTATACACT ATTGGATGTT CTCTACTATG TTACACGTGA TGACTTAAAA
    TGCTTGAGAC TAAGGGGAGG GATGCTGTGC ACACTGTGGA AGGCTATCAT TGACTTTCGA
    AACAAACAGA CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866361.1
    CDS357..4811
    Translation

    Target ORF information:

    RefSeq Version XM_017010872.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA
    CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG
    CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA
    GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT
    GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC
    ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG
    GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT
    GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC
    CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC
    CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA
    ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT
    CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA
    AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT
    AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC
    ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA
    GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC
    ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG
    TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC
    AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC
    TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT
    TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT
    GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG
    TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC
    AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT
    CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG
    CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG
    GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC
    ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG
    TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA
    CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC
    TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG
    GTTCCAGAAC TAAGGCCACA TTTCAGCCTT GCATCTGAGA GTGATACTGC TGATCAAGAA
    GACTTGGATG TAGAAGATGA CCATGAGGAA CAGCCTTCAA ATCAAACTGT CCGAAGACCT
    CAGGCTGTCA TTGAAGATGC TGTGGCTACC TCAGGCGTGA GCACGCTCAG TTCTACTGTG
    TCTCATGATT CCCAGAGTGC TCACCGGTCA CTGAATGTAC AGCTTGGAAG GATGAAAATA
    GAAACCAATA GATTACTGGA AGAATTGGTT CGGAAAGAGA AAGAATTACA AGCACTCCTT
    CATCGAGCTA TTGAAGAAAA AGACCAAGAA ATTAAACACC TGAAGCTTAA GTCCCAACCC
    ATAGAAATTC CTGAATTGCC TGTATTTCAT CTAAATTCTT CTGGCACAAA TACTGAAGAT
    TCTGAACTTA CCGACTGGCT GAGAGTGAAT GGAGCTGATG AAGACACTAT AAGCCGGTTT
    TTGGCTGAAG ATTATACACT ATTGGATGTT CTCTACTATG TTACACGTGA TGACTTAAAA
    TGCTTGAGAC TAAGGGGAGG GATGCTGTGC ACACTGTGGA AGGCTATCAT TGACTTTCGA
    AACAAACAGA CTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96609
    Clone ID Related Accession (Same CDS sequence) XM_017010873.1
    Accession Version XM_017010873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA
    CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG
    CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA
    GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT
    GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC
    ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG
    GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT
    GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC
    CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC
    CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA
    ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT
    CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA
    AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT
    AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC
    ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA
    GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC
    ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG
    TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC
    AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC
    TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT
    TCAGCTCTTT CAGCTGGATC AAATGAATAT CTCAGGAGTA TATCCTTGCC GGTACCTGTG
    CTGGTGGAGG ACACCAGCAG CAGCAGTGAG TACGGCTCAG TTTCACCCGA CACGGAGTTG
    AAAGTGGACC CCTTCTCTTT CAAAACAAGA GCCAAGTCCT GCGGAGAAAG AGATGTCAAG
    GGAATTCGGA CACTCTTTTT GGGCATTCCA GATGAGAATT TTGAAGATCA CAGTGCTCCT
    CCTTCCCCTG AAGAAAAAGA TTCTGGATTC TTCATGCTGA GGAAGGACAG TGAGAGGCGA
    GCTACCCTTC ACAGGATCCT GACGGAAGAC CAAGACAAAA TTGTGAGAAA CCTAATGGAA
    TCTTTAGCTC AGGGGGCTGA AGAACCGAAA CTAAAATGGG AACACATCAC AACCCTCATT
    GCAAGCCTCA GAGAATTTGT GAGATCCACT GACCGAAAAA TCATAGCCAC CACACTGTCA
    AAGCTGAAAC TGGAGCTGGA CTTCGACAGC CATGGCATTA GCCAAGTCCA GGTGGTACTC
    TTTGGTTTTC AAGATGCTGT CAATAAAGTT CTTCGGAATC ATAACATCAA GCCGCACTGG
    ATGTTTGCCT TAGACAGTAT CATTCGGAAG GCGGTACAGA CAGCCATTAC CATCCTGGTT
    CCAGAACTAA GGCCACATTT CAGCCTTGCA TCTGAGAGTG ATACTGCTGA TCAAGAAGAC
    TTGGATGTAG AAGATGACCA TGAGGAACAG CCTTCAAATC AAACTGTCCG AAGACCTCAG
    GCTGTCATTG AAGATGCTGT GGCTACCTCA GGCGTGAGCA CGCTCAGTTC TACTGTGTCT
    CATGATTCCC AGAGTGCTCA CCGGTCACTG AATGTACAGC TTGGAAGGAT GAAAATAGAA
    ACCAATAGAT TACTGGAAGA ATTGGTTCGG AAAGAGAAAG AATTACAAGC ACTCCTTCAT
    CGAGCTATTG AAGAAAAAGA CCAAGAAATT AAACACCTGA AGCTTAAGTC CCAACCCATA
    GAAATTCCTG AATTGCCTGT ATTTCATCTA AATTCTTCTG GCACAAATAC TGAAGATTCT
    GAACTTACCG ACTGGCTGAG AGTGAATGGA GCTGATGAAG ACACTATAAG CCGGTTTTTG
    GCTGAAGATT ATACACTATT GGATGTTCTC TACTATGTTA CACGTGATGA CTTAAAATGC
    TTGAGACTAA GGGGAGGGAT GCTGTGCACA CTGTGGAAGG CTATCATTGA CTTTCGAAAC
    AAACAGACTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866362.1
    CDS356..4807
    Translation

    Target ORF information:

    RefSeq Version XM_017010873.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA
    CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG
    CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA
    GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT
    GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC
    ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG
    GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT
    GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC
    CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC
    CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA
    ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT
    CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA
    AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT
    AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC
    ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA
    GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC
    ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG
    TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC
    AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC
    TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT
    TCAGCTCTTT CAGCTGGATC AAATGAATAT CTCAGGAGTA TATCCTTGCC GGTACCTGTG
    CTGGTGGAGG ACACCAGCAG CAGCAGTGAG TACGGCTCAG TTTCACCCGA CACGGAGTTG
    AAAGTGGACC CCTTCTCTTT CAAAACAAGA GCCAAGTCCT GCGGAGAAAG AGATGTCAAG
    GGAATTCGGA CACTCTTTTT GGGCATTCCA GATGAGAATT TTGAAGATCA CAGTGCTCCT
    CCTTCCCCTG AAGAAAAAGA TTCTGGATTC TTCATGCTGA GGAAGGACAG TGAGAGGCGA
    GCTACCCTTC ACAGGATCCT GACGGAAGAC CAAGACAAAA TTGTGAGAAA CCTAATGGAA
    TCTTTAGCTC AGGGGGCTGA AGAACCGAAA CTAAAATGGG AACACATCAC AACCCTCATT
    GCAAGCCTCA GAGAATTTGT GAGATCCACT GACCGAAAAA TCATAGCCAC CACACTGTCA
    AAGCTGAAAC TGGAGCTGGA CTTCGACAGC CATGGCATTA GCCAAGTCCA GGTGGTACTC
    TTTGGTTTTC AAGATGCTGT CAATAAAGTT CTTCGGAATC ATAACATCAA GCCGCACTGG
    ATGTTTGCCT TAGACAGTAT CATTCGGAAG GCGGTACAGA CAGCCATTAC CATCCTGGTT
    CCAGAACTAA GGCCACATTT CAGCCTTGCA TCTGAGAGTG ATACTGCTGA TCAAGAAGAC
    TTGGATGTAG AAGATGACCA TGAGGAACAG CCTTCAAATC AAACTGTCCG AAGACCTCAG
    GCTGTCATTG AAGATGCTGT GGCTACCTCA GGCGTGAGCA CGCTCAGTTC TACTGTGTCT
    CATGATTCCC AGAGTGCTCA CCGGTCACTG AATGTACAGC TTGGAAGGAT GAAAATAGAA
    ACCAATAGAT TACTGGAAGA ATTGGTTCGG AAAGAGAAAG AATTACAAGC ACTCCTTCAT
    CGAGCTATTG AAGAAAAAGA CCAAGAAATT AAACACCTGA AGCTTAAGTC CCAACCCATA
    GAAATTCCTG AATTGCCTGT ATTTCATCTA AATTCTTCTG GCACAAATAC TGAAGATTCT
    GAACTTACCG ACTGGCTGAG AGTGAATGGA GCTGATGAAG ACACTATAAG CCGGTTTTTG
    GCTGAAGATT ATACACTATT GGATGTTCTC TACTATGTTA CACGTGATGA CTTAAAATGC
    TTGAGACTAA GGGGAGGGAT GCTGTGCACA CTGTGGAAGG CTATCATTGA CTTTCGAAAC
    AAACAGACTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96610
    Clone ID Related Accession (Same CDS sequence) XM_017010874.1
    Accession Version XM_017010874.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAAAA GGTATACATG AGTGGAATTT TAGTGCCTCT
    TCTGTCAGGG GAGTGAGTAT TTCTAAATTT GAAGAAAGAT GCTGCTTTCT TTATGTGCTT
    CACAATTCTG ATGATTTCCA AATCTATTTC TGTACAGAAC TTCATTGTAA AAAGTTTTTT
    GAGATGGTGA ACACCATTAC CGAAGAGAAG GGGAGAAGCA CAGAGGAAGG AGACTGTGAA
    AGTGACTTGC TGGAGTATGA CTATGAATAT GATGAAAATG GTGACAGAGT CGTTTTAGGA
    AAAGGCACTT ATGGGATAGT CTACGCAGGT CGGGACTTGA GCAACCAAGT CAGAATTGCT
    ATTAAGGAAA TCCCAGAGAG AGACAGCAGA TACTCTCAGC CCCTGCATGA AGAAATAGCA
    TTGCATAAAC ACCTGAAGCA CAAAAATATT GTCCAGTATC TGGGCTCTTT CAGTGAGAAT
    GGTTTCATTA AAATCTTCAT GGAGCAGGTC CCTGGAGGAA GTCTTTCTGC TCTCCTTCGT
    TCCAAATGGG GTCCATTAAA AGACAATGAG CAAACAATTG GCTTTTATAC AAAGCAAATA
    CTGGAAGGAT TAAAATATCT CCATGACAAT CAGATAGTTC ACCGGGACAT AAAGGGTGAC
    AATGTGTTGA TTAATACCTA CAGTGGTGTT CTCAAGATCT CTGACTTCGG AACATCAAAG
    AGGCTTGCTG GCATAAACCC CTGTACTGAA ACTTTTACTG GTACCCTCCA GTATATGGCA
    CCAGAAATAA TAGATAAAGG ACCAAGAGGC TACGGAAAAG CAGCAGACAT CTGGTCTCTG
    GGCTGTACAA TCATTGAAAT GGCCACAGGA AAACCCCCAT TTTATGAACT GGGAGAACCA
    CAAGCAGCTA TGTTCAAGGT GGGAATGTTT AAAGTCCACC CTGAGATCCC AGAGTCCATG
    TCTGCAGAGG CCAAGGCATT CATACTGAAA TGTTTTGAAC CAGATCCTGA CAAGAGAGCC
    TGTGCTAACG ACTTGCTTGT TGATGAGTTT TTAAAAGTTT CAAGCAAAAA GAAAAAGACA
    CAACCTAAGC TTTCAGCTCT TTCAGCTGGA TCAAATGCAG AATATCTCAG GAGTATATCC
    TTGCCGGTAC CTGTGCTGGT GGAGGACACC AGCAGCAGCA GTGAGTACGG CTCAGTTTCA
    CCCGACACGG AGTTGAAAGT GGACCCCTTC TCTTTCAAAA CAAGAGCCAA GTCCTGCGGA
    GAAAGAGATG TCAAGGGAAT TCGGACACTC TTTTTGGGCA TTCCAGATGA GAATTTTGAA
    GATCACAGTG CTCCTCCTTC CCCTGAAGAA AAAGATTCTG GATTCTTCAT GCTGAGGAAG
    GACAGTGAGA GGCGAGCTAC CCTTCACAGG ATCCTGACGG AAGACCAAGA CAAAATTGTG
    AGAAACCTAA TGGAATCTTT AGCTCAGGGG GCTGAAGAAC CGAAACTAAA ATGGGAACAC
    ATCACAACCC TCATTGCAAG CCTCAGAGAA TTTGTGAGAT CCACTGACCG AAAAATCATA
    GCCACCACAC TGTCAAAGCT GAAACTGGAG CTGGACTTCG ACAGCCATGG CATTAGCCAA
    GTCCAGGTGG TACTCTTTGG TTTTCAAGAT GCTGTCAATA AAGTTCTTCG GAATCATAAC
    ATCAAGCCGC ACTGGATGTT TGCCTTAGAC AGTATCATTC GGAAGGCGGT ACAGACAGCC
    ATTACCATCC TGGTTCCAGA ACTAAGGCCA CATTTCAGCC TTGCATCTGA GAGTGATACT
    GCTGATCAAG AAGACTTGGA TGTAGAAGAT GACCATGAGG AACAGCCTTC AAATCAAACT
    GTCCGAAGAC CTCAGGCTGT CATTGAAGAT GCTGTGGCTA CCTCAGGCGT GAGCACGCTC
    AGTTCTACTG TGTCTCATGA TTCCCAGAGT GCTCACCGGT CACTGAATGT ACAGCTTGGA
    AGGATGAAAA TAGAAACCAA TAGATTACTG GAAGAATTGG TTCGGAAAGA GAAAGAATTA
    CAAGCACTCC TTCATCGAGC TATTGAAGAA AAAGACCAAG AAATTAAACA CCTGAAGCTT
    AAGTCCCAAC CCATAGAAAT TCCTGAATTG CCTGTATTTC ATCTAAATTC TTCTGGCACA
    AATACTGAAG ATTCTGAACT TACCGACTGG CTGAGAGTGA ATGGAGCTGA TGAAGACACT
    ATAAGCCGGT TTTTGGCTGA AGATTATACA CTATTGGATG TTCTCTACTA TGTTACACGT
    GATGACTTAA AATGCTTGAG ACTAAGGTAC CACTTTTCTT TCTGTTGTCA CTATGGAAAG
    AGCACCAGGC AAATATCAAA AAGTCTGAGT ATCTTCCCAG GCTGGGTCTC TAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866363.1
    CDS358..4734
    Translation

    Target ORF information:

    RefSeq Version XM_017010874.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010874.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAAAA GGTATACATG AGTGGAATTT TAGTGCCTCT
    TCTGTCAGGG GAGTGAGTAT TTCTAAATTT GAAGAAAGAT GCTGCTTTCT TTATGTGCTT
    CACAATTCTG ATGATTTCCA AATCTATTTC TGTACAGAAC TTCATTGTAA AAAGTTTTTT
    GAGATGGTGA ACACCATTAC CGAAGAGAAG GGGAGAAGCA CAGAGGAAGG AGACTGTGAA
    AGTGACTTGC TGGAGTATGA CTATGAATAT GATGAAAATG GTGACAGAGT CGTTTTAGGA
    AAAGGCACTT ATGGGATAGT CTACGCAGGT CGGGACTTGA GCAACCAAGT CAGAATTGCT
    ATTAAGGAAA TCCCAGAGAG AGACAGCAGA TACTCTCAGC CCCTGCATGA AGAAATAGCA
    TTGCATAAAC ACCTGAAGCA CAAAAATATT GTCCAGTATC TGGGCTCTTT CAGTGAGAAT
    GGTTTCATTA AAATCTTCAT GGAGCAGGTC CCTGGAGGAA GTCTTTCTGC TCTCCTTCGT
    TCCAAATGGG GTCCATTAAA AGACAATGAG CAAACAATTG GCTTTTATAC AAAGCAAATA
    CTGGAAGGAT TAAAATATCT CCATGACAAT CAGATAGTTC ACCGGGACAT AAAGGGTGAC
    AATGTGTTGA TTAATACCTA CAGTGGTGTT CTCAAGATCT CTGACTTCGG AACATCAAAG
    AGGCTTGCTG GCATAAACCC CTGTACTGAA ACTTTTACTG GTACCCTCCA GTATATGGCA
    CCAGAAATAA TAGATAAAGG ACCAAGAGGC TACGGAAAAG CAGCAGACAT CTGGTCTCTG
    GGCTGTACAA TCATTGAAAT GGCCACAGGA AAACCCCCAT TTTATGAACT GGGAGAACCA
    CAAGCAGCTA TGTTCAAGGT GGGAATGTTT AAAGTCCACC CTGAGATCCC AGAGTCCATG
    TCTGCAGAGG CCAAGGCATT CATACTGAAA TGTTTTGAAC CAGATCCTGA CAAGAGAGCC
    TGTGCTAACG ACTTGCTTGT TGATGAGTTT TTAAAAGTTT CAAGCAAAAA GAAAAAGACA
    CAACCTAAGC TTTCAGCTCT TTCAGCTGGA TCAAATGCAG AATATCTCAG GAGTATATCC
    TTGCCGGTAC CTGTGCTGGT GGAGGACACC AGCAGCAGCA GTGAGTACGG CTCAGTTTCA
    CCCGACACGG AGTTGAAAGT GGACCCCTTC TCTTTCAAAA CAAGAGCCAA GTCCTGCGGA
    GAAAGAGATG TCAAGGGAAT TCGGACACTC TTTTTGGGCA TTCCAGATGA GAATTTTGAA
    GATCACAGTG CTCCTCCTTC CCCTGAAGAA AAAGATTCTG GATTCTTCAT GCTGAGGAAG
    GACAGTGAGA GGCGAGCTAC CCTTCACAGG ATCCTGACGG AAGACCAAGA CAAAATTGTG
    AGAAACCTAA TGGAATCTTT AGCTCAGGGG GCTGAAGAAC CGAAACTAAA ATGGGAACAC
    ATCACAACCC TCATTGCAAG CCTCAGAGAA TTTGTGAGAT CCACTGACCG AAAAATCATA
    GCCACCACAC TGTCAAAGCT GAAACTGGAG CTGGACTTCG ACAGCCATGG CATTAGCCAA
    GTCCAGGTGG TACTCTTTGG TTTTCAAGAT GCTGTCAATA AAGTTCTTCG GAATCATAAC
    ATCAAGCCGC ACTGGATGTT TGCCTTAGAC AGTATCATTC GGAAGGCGGT ACAGACAGCC
    ATTACCATCC TGGTTCCAGA ACTAAGGCCA CATTTCAGCC TTGCATCTGA GAGTGATACT
    GCTGATCAAG AAGACTTGGA TGTAGAAGAT GACCATGAGG AACAGCCTTC AAATCAAACT
    GTCCGAAGAC CTCAGGCTGT CATTGAAGAT GCTGTGGCTA CCTCAGGCGT GAGCACGCTC
    AGTTCTACTG TGTCTCATGA TTCCCAGAGT GCTCACCGGT CACTGAATGT ACAGCTTGGA
    AGGATGAAAA TAGAAACCAA TAGATTACTG GAAGAATTGG TTCGGAAAGA GAAAGAATTA
    CAAGCACTCC TTCATCGAGC TATTGAAGAA AAAGACCAAG AAATTAAACA CCTGAAGCTT
    AAGTCCCAAC CCATAGAAAT TCCTGAATTG CCTGTATTTC ATCTAAATTC TTCTGGCACA
    AATACTGAAG ATTCTGAACT TACCGACTGG CTGAGAGTGA ATGGAGCTGA TGAAGACACT
    ATAAGCCGGT TTTTGGCTGA AGATTATACA CTATTGGATG TTCTCTACTA TGTTACACGT
    GATGACTTAA AATGCTTGAG ACTAAGGTAC CACTTTTCTT TCTGTTGTCA CTATGGAAAG
    AGCACCAGGC AAATATCAAA AAGTCTGAGT ATCTTCCCAG GCTGGGTCTC TAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96611
    Clone ID Related Accession (Same CDS sequence) XM_017010875.1
    Accession Version XM_017010875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAAAA GGTATACATG AGTGGAATTT TAGTGCCTCT
    TCTGTCAGGG GAGTGAGTAT TTCTAAATTT GAAGAAAGAT GCTGCTTTCT TTATGTGCTT
    CACAATTCTG ATGATTTCCA AATCTATTTC TGTACAGAAC TTCATTGTAA AAAGTTTTTT
    GAGATGGTGA ACACCATTAC CGAAGAGAAG GGGAGAAGCA CAGAGGAAGG AGACTGTGAA
    AGTGACTTGC TGGAGTATGA CTATGAATAT GATGAAAATG GTGACAGAGT CGTTTTAGGA
    AAAGGCACTT ATGGGATAGT CTACGCAGGT CGGGACTTGA GCAACCAAGT CAGAATTGCT
    ATTAAGGAAA TCCCAGAGAG AGACAGCAGA TACTCTCAGC CCCTGCATGA AGAAATAGCA
    TTGCATAAAC ACCTGAAGCA CAAAAATATT GTCCAGTATC TGGGCTCTTT CAGTGAGAAT
    GGTTTCATTA AAATCTTCAT GGAGCAGGTC CCTGGAGGAA GTCTTTCTGC TCTCCTTCGT
    TCCAAATGGG GTCCATTAAA AGACAATGAG CAAACAATTG GCTTTTATAC AAAGCAAATA
    CTGGAAGGAT TAAAATATCT CCATGACAAT CAGATAGTTC ACCGGGACAT AAAGGGTGAC
    AATGTGTTGA TTAATACCTA CAGTGGTGTT CTCAAGATCT CTGACTTCGG AACATCAAAG
    AGGCTTGCTG GCATAAACCC CTGTACTGAA ACTTTTACTG GTACCCTCCA GTATATGGCA
    CCAGAAATAA TAGATAAAGG ACCAAGAGGC TACGGAAAAG CAGCAGACAT CTGGTCTCTG
    GGCTGTACAA TCATTGAAAT GGCCACAGGA AAACCCCCAT TTTATGAACT GGGAGAACCA
    CAAGCAGCTA TGTTCAAGGT GGGAATGTTT AAAGTCCACC CTGAGATCCC AGAGTCCATG
    TCTGCAGAGG CCAAGGCATT CATACTGAAA TGTTTTGAAC CAGATCCTGA CAAGAGAGCC
    TGTGCTAACG ACTTGCTTGT TGATGAGTTT TTAAAAGTTT CAAGCAAAAA GAAAAAGACA
    CAACCTAAGC TTTCAGCTCT TTCAGCTGGA TCAAATGAAT ATCTCAGGAG TATATCCTTG
    CCGGTACCTG TGCTGGTGGA GGACACCAGC AGCAGCAGTG AGTACGGCTC AGTTTCACCC
    GACACGGAGT TGAAAGTGGA CCCCTTCTCT TTCAAAACAA GAGCCAAGTC CTGCGGAGAA
    AGAGATGTCA AGGGAATTCG GACACTCTTT TTGGGCATTC CAGATGAGAA TTTTGAAGAT
    CACAGTGCTC CTCCTTCCCC TGAAGAAAAA GATTCTGGAT TCTTCATGCT GAGGAAGGAC
    AGTGAGAGGC GAGCTACCCT TCACAGGATC CTGACGGAAG ACCAAGACAA AATTGTGAGA
    AACCTAATGG AATCTTTAGC TCAGGGGGCT GAAGAACCGA AACTAAAATG GGAACACATC
    ACAACCCTCA TTGCAAGCCT CAGAGAATTT GTGAGATCCA CTGACCGAAA AATCATAGCC
    ACCACACTGT CAAAGCTGAA ACTGGAGCTG GACTTCGACA GCCATGGCAT TAGCCAAGTC
    CAGGTGGTAC TCTTTGGTTT TCAAGATGCT GTCAATAAAG TTCTTCGGAA TCATAACATC
    AAGCCGCACT GGATGTTTGC CTTAGACAGT ATCATTCGGA AGGCGGTACA GACAGCCATT
    ACCATCCTGG TTCCAGAACT AAGGCCACAT TTCAGCCTTG CATCTGAGAG TGATACTGCT
    GATCAAGAAG ACTTGGATGT AGAAGATGAC CATGAGGAAC AGCCTTCAAA TCAAACTGTC
    CGAAGACCTC AGGCTGTCAT TGAAGATGCT GTGGCTACCT CAGGCGTGAG CACGCTCAGT
    TCTACTGTGT CTCATGATTC CCAGAGTGCT CACCGGTCAC TGAATGTACA GCTTGGAAGG
    ATGAAAATAG AAACCAATAG ATTACTGGAA GAATTGGTTC GGAAAGAGAA AGAATTACAA
    GCACTCCTTC ATCGAGCTAT TGAAGAAAAA GACCAAGAAA TTAAACACCT GAAGCTTAAG
    TCCCAACCCA TAGAAATTCC TGAATTGCCT GTATTTCATC TAAATTCTTC TGGCACAAAT
    ACTGAAGATT CTGAACTTAC CGACTGGCTG AGAGTGAATG GAGCTGATGA AGACACTATA
    AGCCGGTTTT TGGCTGAAGA TTATACACTA TTGGATGTTC TCTACTATGT TACACGTGAT
    GACTTAAAAT GCTTGAGACT AAGGGGAGGG ATGCTGTGCA CACTGTGGAA GGCTATCATT
    GACTTTCGAA ACAAACAGAC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016866364.1
    CDS357..4700
    Translation

    Target ORF information:

    RefSeq Version XM_017010875.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017010875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAAAA GGTATACATG AGTGGAATTT TAGTGCCTCT
    TCTGTCAGGG GAGTGAGTAT TTCTAAATTT GAAGAAAGAT GCTGCTTTCT TTATGTGCTT
    CACAATTCTG ATGATTTCCA AATCTATTTC TGTACAGAAC TTCATTGTAA AAAGTTTTTT
    GAGATGGTGA ACACCATTAC CGAAGAGAAG GGGAGAAGCA CAGAGGAAGG AGACTGTGAA
    AGTGACTTGC TGGAGTATGA CTATGAATAT GATGAAAATG GTGACAGAGT CGTTTTAGGA
    AAAGGCACTT ATGGGATAGT CTACGCAGGT CGGGACTTGA GCAACCAAGT CAGAATTGCT
    ATTAAGGAAA TCCCAGAGAG AGACAGCAGA TACTCTCAGC CCCTGCATGA AGAAATAGCA
    TTGCATAAAC ACCTGAAGCA CAAAAATATT GTCCAGTATC TGGGCTCTTT CAGTGAGAAT
    GGTTTCATTA AAATCTTCAT GGAGCAGGTC CCTGGAGGAA GTCTTTCTGC TCTCCTTCGT
    TCCAAATGGG GTCCATTAAA AGACAATGAG CAAACAATTG GCTTTTATAC AAAGCAAATA
    CTGGAAGGAT TAAAATATCT CCATGACAAT CAGATAGTTC ACCGGGACAT AAAGGGTGAC
    AATGTGTTGA TTAATACCTA CAGTGGTGTT CTCAAGATCT CTGACTTCGG AACATCAAAG
    AGGCTTGCTG GCATAAACCC CTGTACTGAA ACTTTTACTG GTACCCTCCA GTATATGGCA
    CCAGAAATAA TAGATAAAGG ACCAAGAGGC TACGGAAAAG CAGCAGACAT CTGGTCTCTG
    GGCTGTACAA TCATTGAAAT GGCCACAGGA AAACCCCCAT TTTATGAACT GGGAGAACCA
    CAAGCAGCTA TGTTCAAGGT GGGAATGTTT AAAGTCCACC CTGAGATCCC AGAGTCCATG
    TCTGCAGAGG CCAAGGCATT CATACTGAAA TGTTTTGAAC CAGATCCTGA CAAGAGAGCC
    TGTGCTAACG ACTTGCTTGT TGATGAGTTT TTAAAAGTTT CAAGCAAAAA GAAAAAGACA
    CAACCTAAGC TTTCAGCTCT TTCAGCTGGA TCAAATGAAT ATCTCAGGAG TATATCCTTG
    CCGGTACCTG TGCTGGTGGA GGACACCAGC AGCAGCAGTG AGTACGGCTC AGTTTCACCC
    GACACGGAGT TGAAAGTGGA CCCCTTCTCT TTCAAAACAA GAGCCAAGTC CTGCGGAGAA
    AGAGATGTCA AGGGAATTCG GACACTCTTT TTGGGCATTC CAGATGAGAA TTTTGAAGAT
    CACAGTGCTC CTCCTTCCCC TGAAGAAAAA GATTCTGGAT TCTTCATGCT GAGGAAGGAC
    AGTGAGAGGC GAGCTACCCT TCACAGGATC CTGACGGAAG ACCAAGACAA AATTGTGAGA
    AACCTAATGG AATCTTTAGC TCAGGGGGCT GAAGAACCGA AACTAAAATG GGAACACATC
    ACAACCCTCA TTGCAAGCCT CAGAGAATTT GTGAGATCCA CTGACCGAAA AATCATAGCC
    ACCACACTGT CAAAGCTGAA ACTGGAGCTG GACTTCGACA GCCATGGCAT TAGCCAAGTC
    CAGGTGGTAC TCTTTGGTTT TCAAGATGCT GTCAATAAAG TTCTTCGGAA TCATAACATC
    AAGCCGCACT GGATGTTTGC CTTAGACAGT ATCATTCGGA AGGCGGTACA GACAGCCATT
    ACCATCCTGG TTCCAGAACT AAGGCCACAT TTCAGCCTTG CATCTGAGAG TGATACTGCT
    GATCAAGAAG ACTTGGATGT AGAAGATGAC CATGAGGAAC AGCCTTCAAA TCAAACTGTC
    CGAAGACCTC AGGCTGTCAT TGAAGATGCT GTGGCTACCT CAGGCGTGAG CACGCTCAGT
    TCTACTGTGT CTCATGATTC CCAGAGTGCT CACCGGTCAC TGAATGTACA GCTTGGAAGG
    ATGAAAATAG AAACCAATAG ATTACTGGAA GAATTGGTTC GGAAAGAGAA AGAATTACAA
    GCACTCCTTC ATCGAGCTAT TGAAGAAAAA GACCAAGAAA TTAAACACCT GAAGCTTAAG
    TCCCAACCCA TAGAAATTCC TGAATTGCCT GTATTTCATC TAAATTCTTC TGGCACAAAT
    ACTGAAGATT CTGAACTTAC CGACTGGCTG AGAGTGAATG GAGCTGATGA AGACACTATA
    AGCCGGTTTT TGGCTGAAGA TTATACACTA TTGGATGTTC TCTACTATGT TACACGTGAT
    GACTTAAAAT GCTTGAGACT AAGGGGAGGG ATGCTGTGCA CACTGTGGAA GGCTATCATT
    GACTTTCGAA ACAAACAGAC TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96612
    Clone ID Related Accession (Same CDS sequence) XM_011535839.3 , XM_011535839.2
    Accession Version XM_011535839.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011535839.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA
    CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG
    CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA
    GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT
    GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC
    ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG
    GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT
    GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC
    CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC
    CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA
    ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT
    CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA
    AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT
    AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC
    ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA
    GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC
    ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG
    TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC
    AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC
    TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT
    TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT
    GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG
    TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC
    AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT
    CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG
    CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG
    GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC
    ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG
    TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA
    CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC
    TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG
    GTTCCAGATC ATATCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534141.2
    CDS359..4219
    Translation

    Target ORF information:

    RefSeq Version XM_011535839.3
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535839.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA
    CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG
    CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA
    GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT
    GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC
    ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG
    GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT
    GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC
    CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC
    CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA
    ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT
    CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA
    AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT
    AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC
    ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA
    GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC
    ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG
    TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC
    AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC
    TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT
    TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT
    GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG
    TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC
    AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT
    CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG
    CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG
    GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC
    ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG
    TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA
    CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC
    TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG
    GTTCCAGATC ATATCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu96612
    Clone ID Related Accession (Same CDS sequence) XM_011535839.3 , XM_011535839.2
    Accession Version XM_011535839.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mitogen-activated protein kinase kinase kinase 5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535839.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA
    CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG
    CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA
    GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT
    GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC
    ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG
    GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT
    GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC
    CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC
    CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA
    ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT
    CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA
    AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT
    AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC
    ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA
    GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC
    ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG
    TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC
    AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC
    TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT
    TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT
    GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG
    TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC
    AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT
    CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG
    CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG
    GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC
    ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG
    TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA
    CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC
    TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG
    GTTCCAGATC ATATCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011534141.2
    CDS359..4219
    Translation

    Target ORF information:

    RefSeq Version XM_011535839.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011535839.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
    CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG
    GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG
    GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG
    CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC
    GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC
    TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG
    AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG
    GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC
    TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC
    ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG
    GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT
    TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT
    GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG
    AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT
    CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC
    TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG
    AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA
    CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC
    TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA
    TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA
    GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG
    AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG
    AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT
    CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC
    CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC
    AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA
    GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG
    CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA
    CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC
    ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC
    AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG
    CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG
    ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA
    CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG
    CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA
    GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT
    GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC
    ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG
    GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT
    GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC
    CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC
    CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA
    ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT
    CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA
    AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT
    AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC
    ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA
    GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC
    ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG
    TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC
    AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC
    TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT
    TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT
    GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG
    TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC
    AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT
    CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG
    CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG
    GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC
    ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG
    TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA
    CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC
    TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG
    GTTCCAGATC ATATCTTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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