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MIF cDNA ORF clone, Homo sapiens (human)

Gene Symbol MIF
Entrez Gene ID 4282
Full Name macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Synonyms GIF, GLIF, MMIF
General protein information
Preferred Names
macrophage migration inhibitory factor
Names
macrophage migration inhibitory factor
L-dopachrome isomerase
L-dopachrome tautomerase
phenylpyruvate tautomerase
NP_002406.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

22

22q11.23

Summary This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

153620

Disorder Html: {Rheumatoid arthritis, systemic juvenile, susceptibility to},

mRNA and Protein(s)

mRNA Protein Name
NM_002415 NP_002406 macrophage migration inhibitory factor



Homo sapiens (human) MIF NP_002406.1
Macaca mulatta (Rhesus monkey) MIF NP_001028087.1
Bos taurus (cattle) MIF NP_001028780.1
Mus musculus (house mouse) Mif NP_034928.1
Rattus norvegicus (Norway rat) Mif NP_112313.1
Gallus gallus (chicken) MIF XP_003642282.1
Danio rerio (zebrafish) mif NP_001036786.1
Xenopus (Silurana) tropicalis (western clawed frog) mif NP_001107147.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MIF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu25891
NM_002415 Homo sapiens macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu25891
Clone ID Related Accession (Same CDS sequence) NM_002415
Accession Version NM_002415.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 348bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product macrophage migration inhibitory factor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L19686.1. This sequence is a reference standard in the RefSeqGene project. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU599051.1, BC022414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
ATGCCGATGT TCATCGTAAA CACCAACGTG CCCCGCGCCT CCGTGCCGGA CGGGTTCCTC 
TCCGAGCTCA CCCAGCAGCT GGCGCAGGCC ACCGGCAAGC CCCCCCAGTA CATCGCGGTG
CACGTGGTCC CGGACCAGCT CATGGCCTTC GGCGGCTCCA GCGAGCCGTG CGCGCTCTGC
AGCCTGCACA GCATCGGCAA GATCGGCGGC GCGCAGAACC GCTCCTACAG CAAGCTGCTG
TGCGGCCTGC TGGCCGAGCG CCTGCGCATC AGCCCGGACA GGGTCTACAT CAACTATTAC
GACATGAACG CGGCCAATGT GGGCTGGAAC AACTCCACCT TCGCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002406.1
CDS98..445
Misc Feature(1)101..442(+)
Misc Feature(2)206..208(+)
Misc Feature(3)329..331(+)
Exon (1)1..205
Gene:MIF
Gene Synonym:
Exon (2)206..378
Gene:MIF
Gene Synonym:
Exon (3)379..561
Gene:MIF
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_002415
Organism Homo sapiens (human)
Definition Homo sapiens macrophage migration inhibitory factor (glycosylation-inhibiting factor) (MIF), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002415

ORF Insert Sequence:

1
61
121
181
241
301
ATGCCGATGT TCATCGTAAA CACCAACGTG CCCCGCGCCT CCGTGCCGGA CGGGTTCCTC 
TCCGAGCTCA CCCAGCAGCT GGCGCAGGCC ACCGGCAAGC CCCCCCAGTA CATCGCGGTG
CACGTGGTCC CGGACCAGCT CATGGCCTTC GGCGGCTCCA GCGAGCCGTG CGCGCTCTGC
AGCCTGCACA GCATCGGCAA GATCGGCGGC GCGCAGAACC GCTCCTACAG CAAGCTGCTG
TGCGGCCTGC TGGCCGAGCG CCTGCGCATC AGCCCGGACA GGGTCTACAT CAACTATTAC
GACATGAACG CGGCCAATGT GGGCTGGAAC AACTCCACCT TCGCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Macrophage migration inhibitory factor deficiency in chronic obstructive pulmonary disease
Am. J. Physiol. Lung Cell Mol. Physiol. 306 (6), L487-L496 (2014)
Sauler M, Leng L, Trentalange M, Haslip M, Shan P, Piecychna M, Zhang Y, Andrews N, Mannam P, Allore H, Fried T, Bucala R and Lee PJ.


book

Dysregulated D-dopachrome tautomerase, a hypoxia-inducible factor-dependent gene, cooperates with macrophage migration inhibitory factor in renal tumorigenesis
J. Biol. Chem. 289 (6), 3713-3723 (2014)
Pasupuleti V, Du W, Gupta Y, Yeh IJ, Montano M, Magi-Galuzzi C and Welford SM.


book

MIF gene polymorphisms confer susceptibility to Vogt-Koyanagi-Harada syndrome in a Han Chinese population
Invest. Ophthalmol. Vis. Sci. 54 (12), 7734-7738 (2013)
Zhang C, Liu S, Hou S, Lei B, Zheng X, Xiao X, Kijlstra A and Yang P.


book

Macrophage migration inhibitory factor gene polymorphisms in inflammatory bowel disease: an association study in New Zealand Caucasians and meta-analysis
World J. Gastroenterol. 19 (39), 6656-6664 (2013)
Falvey JD, Bentley RW, Merriman TR, Hampton MB, Barclay ML, Gearry RB and Roberts RL.


book

Polymorphism and expression of macrophage migration inhibitory factor does not contribute to glucocorticoid resistance in idiopathic thrombocytopenic purpura
Pharmazie 68 (10), 846-849 (2013)
Lao W, Xiang Y, Fang M and Yang X.


book

Genomic cloning of mouse MIF (macrophage inhibitory factor) and genetic mapping of the human and mouse expressed gene and nine mouse pseudogenes
Genomics 27 (3), 405-411 (1995)
Kozak CA, Adamson MC, Buckler CE, Segovia L, Paralkar V and Wistow G.


book

The major binding protein of the interferon antagonist sarcolectin in human placenta is a macrophage migration inhibitory factor
Arch. Biochem. Biophys. 303 (1), 74-80 (1993)
Zeng FY, Weiser WY, Kratzin H, Stahl B, Karas M and Gabius HJ.


book

Human liver protein map: a reference database established by microsequencing and gel comparison
Electrophoresis 13 (12), 992-1001 (1992)
Hochstrasser DF, Frutiger S, Paquet N, Bairoch A, Ravier F, Pasquali C, Sanchez JC, Tissot JD, Bjellqvist B and Vargas R.


book

Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor
Proc. Natl. Acad. Sci. U.S.A. 86 (19), 7522-7526 (1989)
Weiser WY, Temple PA, Witek-Giannotti JS, Remold HG, Clark SC and David JR.


book

Glycolipid receptor for human migration inhibitory factor: fucose and sialic acid are important for the human monocyte response to migration inhibitory factor
Cell. Immunol. 90 (2), 539-546 (1985)
Liu,D.Y., Yu,S.F., Remold,H.G. and David,J.R.


 
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