MTHFR cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following MTHFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu24181 NM_005957.5
Latest version!
Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu33449 NM_001330358.1
Latest version!
Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$279.50-$391.30
$559.00
OHu24181 XM_005263462.4
Latest version!
Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu24181 XM_005263461.3
Latest version!
Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu24181 XM_005263460.5
Latest version!
Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu33450 XM_024447198.1
Latest version!
Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu33450 XM_005263463.4
Latest version!
Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu59084 XM_011541495.3
Latest version!
Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu59085 XM_011541496.3
Latest version!
Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu90042 XM_017001328.2
Latest version!
Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OHu90042 XM_017001328.1 Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OHu24181 XM_005263460.3 Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu24181 XM_005263460.4 Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu33450 XM_005263463.3 Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OHu59084 XM_011541495.2 Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu24181 XM_005263462.3 Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu24181 NM_005957.4 Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu59085 XM_011541496.2 Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu24181
    Clone ID Related Accession (Same CDS sequence) XM_005263462.3 , XM_005263460.4 , NM_005957.5 , XM_005263460.3 , XM_005263460.5 , XM_005263462.4 , NM_005957.4 , XM_005263461.3
    Accession Version NM_005957.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY046560.1, AB209113.1, AL953897.6, AJ237672.1 and BC053509.1. On Aug 31, 2019 this sequence version replaced NM_005957.4. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053509.1, AK312907.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005948.3
    CDS91..2061
    Translation

    Target ORF information:

    RefSeq Version NM_005957.5
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005957.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24181
    Clone ID Related Accession (Same CDS sequence) XM_005263462.3 , XM_005263460.4 , NM_005957.5 , XM_005263460.3 , XM_005263460.5 , XM_005263462.4 , NM_005957.4 , XM_005263461.3
    Accession Version NM_005957.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-01
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY046560.1, AB209113.1, AL953897.6, AJ237672.1 and BC053509.1. This sequence is a reference standard in the RefSeqGene project. On Oct 9, 2009 this sequence version replaced NM_005957.3. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053509.1, SRR1803615.97218.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_005948.3
    CDS230..2200
    Translation

    Target ORF information:

    RefSeq Version NM_005957.4
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_005957.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu33449
    Clone ID Related Accession (Same CDS sequence) NM_001330358.1
    Accession Version NM_001330358.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL953897.6. On Aug 29, 2016 this sequence version replaced XM_005263458.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.37518.1, SRR1803612.71433.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMN03465406 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG TAGGAACCCA
    GCCATGGTGA ACGAAGCCAG AGGAAACAGC AGCCTCAACC CCTGCTTGGA GGGCAGTGCC
    AGCAGTGGCA GTGAGAGCTC CAAAGATAGT TCGAGATGTT CCACCCCGGG CCTGGACCCC
    GAGCGGCATG AGAGACTCCG GGAGAAGATG AGGCGGCGAT TGGAATCTGG TGACAAGTGG
    TTCTCCCTGG AATTCTTCCC TCCTCGAACT GCTGAGGGAG CTGTCAATCT CATCTCAAGG
    TTTGACCGGA TGGCAGCAGG TGGCCCCCTC TACATAGACG TGACCTGGCA CCCAGCAGGT
    GACCCTGGCT CAGACAAGGA GACCTCCTCC ATGATGATCG CCAGCACCGC CGTGAACTAC
    TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCCGTC AGCGCCTGGA GGAGATCACG
    GGCCATCTGC ACAAAGCTAA GCAGCTGGGC CTGAAGAACA TCATGGCGCT GCGGGGAGAC
    CCAATAGGTG ACCAGTGGGA AGAGGAGGAG GGAGGCTTCA ACTACGCAGT GGACCTGGTG
    AAGCACATCC GAAGTGAGTT TGGTGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA
    GGCCACCCCG AAGCAGGGAG CTTTGAGGCT GACCTGAAGC ACTTGAAGGA GAAGGTGTCT
    GCGGGAGCCG ATTTCATCAT CACGCAGCTT TTCTTTGAGG CTGACACATT CTTCCGCTTT
    GTGAAGGCAT GCACCGACAT GGGCATCACT TGCCCCATCG TCCCCGGGAT CTTTCCCATC
    CAGGGCTACC ACTCCCTTCG GCAGCTTGTG AAGCTGTCCA AGCTGGAGGT GCCACAGGAG
    ATCAAGGACG TGATTGAGCC AATCAAAGAC AACGATGCTG CCATCCGCAA CTATGGCATC
    GAGCTGGCCG TGAGCCTGTG CCAGGAGCTT CTGGCCAGTG GCTTGGTGCC AGGCCTCCAC
    TTCTACACCC TCAACCGCGA GATGGCTACC ACAGAGGTGC TGAAGCGCCT GGGGATGTGG
    ACTGAGGACC CCAGGCGTCC CCTACCCTGG GCTCTCAGCG CCCACCCCAA GCGCCGAGAG
    GAAGATGTAC GTCCCATCTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACCCAG
    GAGTGGGACG AGTTCCCTAA CGGCCGCTGG GGCAATTCCT CTTCCCCTGC CTTTGGGGAG
    CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CCAAGGAGGA GCTGCTGAAG
    ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGTGTCTTTG AAGTCTTCGT TCTTTACCTC
    TCGGGAGAAC CAAACCGGAA TGGTCACAAA GTGACTTGCC TGCCCTGGAA CGATGAGCCC
    CTGGCGGCTG AGACCAGCCT GCTGAAGGAG GAGCTGCTGC GGGTGAACCG CCAGGGCATC
    CTCACCATCA ACTCACAGCC CAACATCAAC GGGAAGCCGT CCTCCGACCC CATCGTGGGC
    TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TAGAGTTTTT CACTTCCCGC
    GAGACAGCGG AAGCACTTCT GCAAGTGCTG AAGAAGTACG AGCTCCGGGT TAATTACCAC
    CTTGTCAATG TGAAGGGTGA AAACATCACC AATGCCCCTG AACTGCAGCC GAATGCTGTC
    ACTTGGGGCA TCTTCCCTGG GCGAGAGATC ATCCAGCCCA CCGTAGTGGA TCCCGTCAGC
    TTCATGTTCT GGAAGGACGA GGCCTTTGCC CTGTGGATTG AGCGGTGGGG AAAGCTGTAT
    GAGGAGGAGT CCCCGTCCCG CACCATCATC CAGTACATCC ACGACAACTA CTTCCTGGTC
    AACCTGGTGG ACAATGACTT CCCACTGGAC AACTGCCTCT GGCAGGTGGT GGAAGACACA
    TTGGAGCTTC TCAACAGGCC CACCCAGAAT GCGAGAGAAA CGGAGGCTCC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317287.1
    CDS164..2257
    Translation

    Target ORF information:

    RefSeq Version NM_001330358.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330358.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG TAGGAACCCA
    GCCATGGTGA ACGAAGCCAG AGGAAACAGC AGCCTCAACC CCTGCTTGGA GGGCAGTGCC
    AGCAGTGGCA GTGAGAGCTC CAAAGATAGT TCGAGATGTT CCACCCCGGG CCTGGACCCC
    GAGCGGCATG AGAGACTCCG GGAGAAGATG AGGCGGCGAT TGGAATCTGG TGACAAGTGG
    TTCTCCCTGG AATTCTTCCC TCCTCGAACT GCTGAGGGAG CTGTCAATCT CATCTCAAGG
    TTTGACCGGA TGGCAGCAGG TGGCCCCCTC TACATAGACG TGACCTGGCA CCCAGCAGGT
    GACCCTGGCT CAGACAAGGA GACCTCCTCC ATGATGATCG CCAGCACCGC CGTGAACTAC
    TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCCGTC AGCGCCTGGA GGAGATCACG
    GGCCATCTGC ACAAAGCTAA GCAGCTGGGC CTGAAGAACA TCATGGCGCT GCGGGGAGAC
    CCAATAGGTG ACCAGTGGGA AGAGGAGGAG GGAGGCTTCA ACTACGCAGT GGACCTGGTG
    AAGCACATCC GAAGTGAGTT TGGTGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA
    GGCCACCCCG AAGCAGGGAG CTTTGAGGCT GACCTGAAGC ACTTGAAGGA GAAGGTGTCT
    GCGGGAGCCG ATTTCATCAT CACGCAGCTT TTCTTTGAGG CTGACACATT CTTCCGCTTT
    GTGAAGGCAT GCACCGACAT GGGCATCACT TGCCCCATCG TCCCCGGGAT CTTTCCCATC
    CAGGGCTACC ACTCCCTTCG GCAGCTTGTG AAGCTGTCCA AGCTGGAGGT GCCACAGGAG
    ATCAAGGACG TGATTGAGCC AATCAAAGAC AACGATGCTG CCATCCGCAA CTATGGCATC
    GAGCTGGCCG TGAGCCTGTG CCAGGAGCTT CTGGCCAGTG GCTTGGTGCC AGGCCTCCAC
    TTCTACACCC TCAACCGCGA GATGGCTACC ACAGAGGTGC TGAAGCGCCT GGGGATGTGG
    ACTGAGGACC CCAGGCGTCC CCTACCCTGG GCTCTCAGCG CCCACCCCAA GCGCCGAGAG
    GAAGATGTAC GTCCCATCTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACCCAG
    GAGTGGGACG AGTTCCCTAA CGGCCGCTGG GGCAATTCCT CTTCCCCTGC CTTTGGGGAG
    CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CCAAGGAGGA GCTGCTGAAG
    ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGTGTCTTTG AAGTCTTCGT TCTTTACCTC
    TCGGGAGAAC CAAACCGGAA TGGTCACAAA GTGACTTGCC TGCCCTGGAA CGATGAGCCC
    CTGGCGGCTG AGACCAGCCT GCTGAAGGAG GAGCTGCTGC GGGTGAACCG CCAGGGCATC
    CTCACCATCA ACTCACAGCC CAACATCAAC GGGAAGCCGT CCTCCGACCC CATCGTGGGC
    TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TAGAGTTTTT CACTTCCCGC
    GAGACAGCGG AAGCACTTCT GCAAGTGCTG AAGAAGTACG AGCTCCGGGT TAATTACCAC
    CTTGTCAATG TGAAGGGTGA AAACATCACC AATGCCCCTG AACTGCAGCC GAATGCTGTC
    ACTTGGGGCA TCTTCCCTGG GCGAGAGATC ATCCAGCCCA CCGTAGTGGA TCCCGTCAGC
    TTCATGTTCT GGAAGGACGA GGCCTTTGCC CTGTGGATTG AGCGGTGGGG AAAGCTGTAT
    GAGGAGGAGT CCCCGTCCCG CACCATCATC CAGTACATCC ACGACAACTA CTTCCTGGTC
    AACCTGGTGG ACAATGACTT CCCACTGGAC AACTGCCTCT GGCAGGTGGT GGAAGACACA
    TTGGAGCTTC TCAACAGGCC CACCCAGAAT GCGAGAGAAA CGGAGGCTCC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24181
    Clone ID Related Accession (Same CDS sequence) XM_005263462.3 , XM_005263460.4 , NM_005957.5 , XM_005263460.3 , XM_005263460.5 , XM_005263462.4 , NM_005957.4 , XM_005263461.3
    Accession Version XM_005263462.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product methylenetetrahydrofolate reductase isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263519.1
    Translation

    Target ORF information:

    RefSeq Version XM_005263462.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263462.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24181
    Clone ID Related Accession (Same CDS sequence) XM_005263462.3 , XM_005263460.4 , NM_005957.5 , XM_005263460.3 , XM_005263460.5 , XM_005263462.4 , NM_005957.4 , XM_005263461.3
    Accession Version XM_005263460.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263460.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263517.1
    CDS685..2655
    Translation

    Target ORF information:

    RefSeq Version XM_005263460.4
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263460.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24181
    Clone ID Related Accession (Same CDS sequence) XM_005263462.3 , XM_005263460.4 , NM_005957.5 , XM_005263460.3 , XM_005263460.5 , XM_005263462.4 , NM_005957.4 , XM_005263461.3
    Accession Version XM_005263460.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product methylenetetrahydrofolate reductase isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263517.1
    Translation

    Target ORF information:

    RefSeq Version XM_005263460.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263460.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24181
    Clone ID Related Accession (Same CDS sequence) XM_005263462.3 , XM_005263460.4 , NM_005957.5 , XM_005263460.3 , XM_005263460.5 , XM_005263462.4 , NM_005957.4 , XM_005263461.3
    Accession Version XM_005263460.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263460.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263517.1
    CDS617..2587
    Translation

    Target ORF information:

    RefSeq Version XM_005263460.5
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263460.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24181
    Clone ID Related Accession (Same CDS sequence) XM_005263462.3 , XM_005263460.4 , NM_005957.5 , XM_005263460.3 , XM_005263460.5 , XM_005263462.4 , NM_005957.4 , XM_005263461.3
    Accession Version XM_005263462.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263462.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263519.1
    CDS118..2088
    Translation

    Target ORF information:

    RefSeq Version XM_005263462.4
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263462.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu24181
    Clone ID Related Accession (Same CDS sequence) XM_005263462.3 , XM_005263460.4 , NM_005957.5 , XM_005263460.3 , XM_005263460.5 , XM_005263462.4 , NM_005957.4 , XM_005263461.3
    Accession Version XM_005263461.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product methylenetetrahydrofolate reductase isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263518.1
    Translation

    Target ORF information:

    RefSeq Version XM_005263461.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263461.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC 
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu33450
    Clone ID Related Accession (Same CDS sequence) XM_005263463.4 , XM_005263463.3 , XM_024447198.1
    Accession Version XM_005263463.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263463.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCAGCAG GTGGCCCCCT CTACATAGAC GTGACCTGGC ACCCAGCAGG TGACCCTGGC 
    TCAGACAAGG AGACCTCCTC CATGATGATC GCCAGCACCG CCGTGAACTA CTGTGGCCTG
    GAGACCATCC TGCACATGAC CTGCTGCCGT CAGCGCCTGG AGGAGATCAC GGGCCATCTG
    CACAAAGCTA AGCAGCTGGG CCTGAAGAAC ATCATGGCGC TGCGGGGAGA CCCAATAGGT
    GACCAGTGGG AAGAGGAGGA GGGAGGCTTC AACTACGCAG TGGACCTGGT GAAGCACATC
    CGAAGTGAGT TTGGTGACTA CTTTGACATC TGTGTGGCAG GTTACCCCAA AGGCCACCCC
    GAAGCAGGGA GCTTTGAGGC TGACCTGAAG CACTTGAAGG AGAAGGTGTC TGCGGGAGCC
    GATTTCATCA TCACGCAGCT TTTCTTTGAG GCTGACACAT TCTTCCGCTT TGTGAAGGCA
    TGCACCGACA TGGGCATCAC TTGCCCCATC GTCCCCGGGA TCTTTCCCAT CCAGGGCTAC
    CACTCCCTTC GGCAGCTTGT GAAGCTGTCC AAGCTGGAGG TGCCACAGGA GATCAAGGAC
    GTGATTGAGC CAATCAAAGA CAACGATGCT GCCATCCGCA ACTATGGCAT CGAGCTGGCC
    GTGAGCCTGT GCCAGGAGCT TCTGGCCAGT GGCTTGGTGC CAGGCCTCCA CTTCTACACC
    CTCAACCGCG AGATGGCTAC CACAGAGGTG CTGAAGCGCC TGGGGATGTG GACTGAGGAC
    CCCAGGCGTC CCCTACCCTG GGCTCTCAGC GCCCACCCCA AGCGCCGAGA GGAAGATGTA
    CGTCCCATCT TCTGGGCCTC CAGACCAAAG AGTTACATCT ACCGTACCCA GGAGTGGGAC
    GAGTTCCCTA ACGGCCGCTG GGGCAATTCC TCTTCCCCTG CCTTTGGGGA GCTGAAGGAC
    TACTACCTCT TCTACCTGAA GAGCAAGTCC CCCAAGGAGG AGCTGCTGAA GATGTGGGGG
    GAGGAGCTGA CCAGTGAAGA AAGTGTCTTT GAAGTCTTCG TTCTTTACCT CTCGGGAGAA
    CCAAACCGGA ATGGTCACAA AGTGACTTGC CTGCCCTGGA ACGATGAGCC CCTGGCGGCT
    GAGACCAGCC TGCTGAAGGA GGAGCTGCTG CGGGTGAACC GCCAGGGCAT CCTCACCATC
    AACTCACAGC CCAACATCAA CGGGAAGCCG TCCTCCGACC CCATCGTGGG CTGGGGCCCC
    AGCGGGGGCT ATGTCTTCCA GAAGGCCTAC TTAGAGTTTT TCACTTCCCG CGAGACAGCG
    GAAGCACTTC TGCAAGTGCT GAAGAAGTAC GAGCTCCGGG TTAATTACCA CCTTGTCAAT
    GTGAAGGGTG AAAACATCAC CAATGCCCCT GAACTGCAGC CGAATGCTGT CACTTGGGGC
    ATCTTCCCTG GGCGAGAGAT CATCCAGCCC ACCGTAGTGG ATCCCGTCAG CTTCATGTTC
    TGGAAGGACG AGGCCTTTGC CCTGTGGATT GAGCGGTGGG GAAAGCTGTA TGAGGAGGAG
    TCCCCGTCCC GCACCATCAT CCAGTACATC CACGACAACT ACTTCCTGGT CAACCTGGTG
    GACAATGACT TCCCACTGGA CAACTGCCTC TGGCAGGTGG TGGAAGACAC ATTGGAGCTT
    CTCAACAGGC CCACCCAGAA TGCGAGAGAA ACGGAGGCTC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263520.1
    CDS493..2217
    Translation

    Target ORF information:

    RefSeq Version XM_005263463.4
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263463.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCAGCAG GTGGCCCCCT CTACATAGAC GTGACCTGGC ACCCAGCAGG TGACCCTGGC 
    TCAGACAAGG AGACCTCCTC CATGATGATC GCCAGCACCG CCGTGAACTA CTGTGGCCTG
    GAGACCATCC TGCACATGAC CTGCTGCCGT CAGCGCCTGG AGGAGATCAC GGGCCATCTG
    CACAAAGCTA AGCAGCTGGG CCTGAAGAAC ATCATGGCGC TGCGGGGAGA CCCAATAGGT
    GACCAGTGGG AAGAGGAGGA GGGAGGCTTC AACTACGCAG TGGACCTGGT GAAGCACATC
    CGAAGTGAGT TTGGTGACTA CTTTGACATC TGTGTGGCAG GTTACCCCAA AGGCCACCCC
    GAAGCAGGGA GCTTTGAGGC TGACCTGAAG CACTTGAAGG AGAAGGTGTC TGCGGGAGCC
    GATTTCATCA TCACGCAGCT TTTCTTTGAG GCTGACACAT TCTTCCGCTT TGTGAAGGCA
    TGCACCGACA TGGGCATCAC TTGCCCCATC GTCCCCGGGA TCTTTCCCAT CCAGGGCTAC
    CACTCCCTTC GGCAGCTTGT GAAGCTGTCC AAGCTGGAGG TGCCACAGGA GATCAAGGAC
    GTGATTGAGC CAATCAAAGA CAACGATGCT GCCATCCGCA ACTATGGCAT CGAGCTGGCC
    GTGAGCCTGT GCCAGGAGCT TCTGGCCAGT GGCTTGGTGC CAGGCCTCCA CTTCTACACC
    CTCAACCGCG AGATGGCTAC CACAGAGGTG CTGAAGCGCC TGGGGATGTG GACTGAGGAC
    CCCAGGCGTC CCCTACCCTG GGCTCTCAGC GCCCACCCCA AGCGCCGAGA GGAAGATGTA
    CGTCCCATCT TCTGGGCCTC CAGACCAAAG AGTTACATCT ACCGTACCCA GGAGTGGGAC
    GAGTTCCCTA ACGGCCGCTG GGGCAATTCC TCTTCCCCTG CCTTTGGGGA GCTGAAGGAC
    TACTACCTCT TCTACCTGAA GAGCAAGTCC CCCAAGGAGG AGCTGCTGAA GATGTGGGGG
    GAGGAGCTGA CCAGTGAAGA AAGTGTCTTT GAAGTCTTCG TTCTTTACCT CTCGGGAGAA
    CCAAACCGGA ATGGTCACAA AGTGACTTGC CTGCCCTGGA ACGATGAGCC CCTGGCGGCT
    GAGACCAGCC TGCTGAAGGA GGAGCTGCTG CGGGTGAACC GCCAGGGCAT CCTCACCATC
    AACTCACAGC CCAACATCAA CGGGAAGCCG TCCTCCGACC CCATCGTGGG CTGGGGCCCC
    AGCGGGGGCT ATGTCTTCCA GAAGGCCTAC TTAGAGTTTT TCACTTCCCG CGAGACAGCG
    GAAGCACTTC TGCAAGTGCT GAAGAAGTAC GAGCTCCGGG TTAATTACCA CCTTGTCAAT
    GTGAAGGGTG AAAACATCAC CAATGCCCCT GAACTGCAGC CGAATGCTGT CACTTGGGGC
    ATCTTCCCTG GGCGAGAGAT CATCCAGCCC ACCGTAGTGG ATCCCGTCAG CTTCATGTTC
    TGGAAGGACG AGGCCTTTGC CCTGTGGATT GAGCGGTGGG GAAAGCTGTA TGAGGAGGAG
    TCCCCGTCCC GCACCATCAT CCAGTACATC CACGACAACT ACTTCCTGGT CAACCTGGTG
    GACAATGACT TCCCACTGGA CAACTGCCTC TGGCAGGTGG TGGAAGACAC ATTGGAGCTT
    CTCAACAGGC CCACCCAGAA TGCGAGAGAA ACGGAGGCTC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu33450
    Clone ID Related Accession (Same CDS sequence) XM_005263463.4 , XM_005263463.3 , XM_024447198.1
    Accession Version XM_005263463.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263463.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCAGCAG GTGGCCCCCT CTACATAGAC GTGACCTGGC ACCCAGCAGG TGACCCTGGC 
    TCAGACAAGG AGACCTCCTC CATGATGATC GCCAGCACCG CCGTGAACTA CTGTGGCCTG
    GAGACCATCC TGCACATGAC CTGCTGCCGT CAGCGCCTGG AGGAGATCAC GGGCCATCTG
    CACAAAGCTA AGCAGCTGGG CCTGAAGAAC ATCATGGCGC TGCGGGGAGA CCCAATAGGT
    GACCAGTGGG AAGAGGAGGA GGGAGGCTTC AACTACGCAG TGGACCTGGT GAAGCACATC
    CGAAGTGAGT TTGGTGACTA CTTTGACATC TGTGTGGCAG GTTACCCCAA AGGCCACCCC
    GAAGCAGGGA GCTTTGAGGC TGACCTGAAG CACTTGAAGG AGAAGGTGTC TGCGGGAGCC
    GATTTCATCA TCACGCAGCT TTTCTTTGAG GCTGACACAT TCTTCCGCTT TGTGAAGGCA
    TGCACCGACA TGGGCATCAC TTGCCCCATC GTCCCCGGGA TCTTTCCCAT CCAGGGCTAC
    CACTCCCTTC GGCAGCTTGT GAAGCTGTCC AAGCTGGAGG TGCCACAGGA GATCAAGGAC
    GTGATTGAGC CAATCAAAGA CAACGATGCT GCCATCCGCA ACTATGGCAT CGAGCTGGCC
    GTGAGCCTGT GCCAGGAGCT TCTGGCCAGT GGCTTGGTGC CAGGCCTCCA CTTCTACACC
    CTCAACCGCG AGATGGCTAC CACAGAGGTG CTGAAGCGCC TGGGGATGTG GACTGAGGAC
    CCCAGGCGTC CCCTACCCTG GGCTCTCAGC GCCCACCCCA AGCGCCGAGA GGAAGATGTA
    CGTCCCATCT TCTGGGCCTC CAGACCAAAG AGTTACATCT ACCGTACCCA GGAGTGGGAC
    GAGTTCCCTA ACGGCCGCTG GGGCAATTCC TCTTCCCCTG CCTTTGGGGA GCTGAAGGAC
    TACTACCTCT TCTACCTGAA GAGCAAGTCC CCCAAGGAGG AGCTGCTGAA GATGTGGGGG
    GAGGAGCTGA CCAGTGAAGA AAGTGTCTTT GAAGTCTTCG TTCTTTACCT CTCGGGAGAA
    CCAAACCGGA ATGGTCACAA AGTGACTTGC CTGCCCTGGA ACGATGAGCC CCTGGCGGCT
    GAGACCAGCC TGCTGAAGGA GGAGCTGCTG CGGGTGAACC GCCAGGGCAT CCTCACCATC
    AACTCACAGC CCAACATCAA CGGGAAGCCG TCCTCCGACC CCATCGTGGG CTGGGGCCCC
    AGCGGGGGCT ATGTCTTCCA GAAGGCCTAC TTAGAGTTTT TCACTTCCCG CGAGACAGCG
    GAAGCACTTC TGCAAGTGCT GAAGAAGTAC GAGCTCCGGG TTAATTACCA CCTTGTCAAT
    GTGAAGGGTG AAAACATCAC CAATGCCCCT GAACTGCAGC CGAATGCTGT CACTTGGGGC
    ATCTTCCCTG GGCGAGAGAT CATCCAGCCC ACCGTAGTGG ATCCCGTCAG CTTCATGTTC
    TGGAAGGACG AGGCCTTTGC CCTGTGGATT GAGCGGTGGG GAAAGCTGTA TGAGGAGGAG
    TCCCCGTCCC GCACCATCAT CCAGTACATC CACGACAACT ACTTCCTGGT CAACCTGGTG
    GACAATGACT TCCCACTGGA CAACTGCCTC TGGCAGGTGG TGGAAGACAC ATTGGAGCTT
    CTCAACAGGC CCACCCAGAA TGCGAGAGAA ACGGAGGCTC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263520.1
    CDS493..2217
    Translation

    Target ORF information:

    RefSeq Version XM_005263463.3
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263463.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCAGCAG GTGGCCCCCT CTACATAGAC GTGACCTGGC ACCCAGCAGG TGACCCTGGC 
    TCAGACAAGG AGACCTCCTC CATGATGATC GCCAGCACCG CCGTGAACTA CTGTGGCCTG
    GAGACCATCC TGCACATGAC CTGCTGCCGT CAGCGCCTGG AGGAGATCAC GGGCCATCTG
    CACAAAGCTA AGCAGCTGGG CCTGAAGAAC ATCATGGCGC TGCGGGGAGA CCCAATAGGT
    GACCAGTGGG AAGAGGAGGA GGGAGGCTTC AACTACGCAG TGGACCTGGT GAAGCACATC
    CGAAGTGAGT TTGGTGACTA CTTTGACATC TGTGTGGCAG GTTACCCCAA AGGCCACCCC
    GAAGCAGGGA GCTTTGAGGC TGACCTGAAG CACTTGAAGG AGAAGGTGTC TGCGGGAGCC
    GATTTCATCA TCACGCAGCT TTTCTTTGAG GCTGACACAT TCTTCCGCTT TGTGAAGGCA
    TGCACCGACA TGGGCATCAC TTGCCCCATC GTCCCCGGGA TCTTTCCCAT CCAGGGCTAC
    CACTCCCTTC GGCAGCTTGT GAAGCTGTCC AAGCTGGAGG TGCCACAGGA GATCAAGGAC
    GTGATTGAGC CAATCAAAGA CAACGATGCT GCCATCCGCA ACTATGGCAT CGAGCTGGCC
    GTGAGCCTGT GCCAGGAGCT TCTGGCCAGT GGCTTGGTGC CAGGCCTCCA CTTCTACACC
    CTCAACCGCG AGATGGCTAC CACAGAGGTG CTGAAGCGCC TGGGGATGTG GACTGAGGAC
    CCCAGGCGTC CCCTACCCTG GGCTCTCAGC GCCCACCCCA AGCGCCGAGA GGAAGATGTA
    CGTCCCATCT TCTGGGCCTC CAGACCAAAG AGTTACATCT ACCGTACCCA GGAGTGGGAC
    GAGTTCCCTA ACGGCCGCTG GGGCAATTCC TCTTCCCCTG CCTTTGGGGA GCTGAAGGAC
    TACTACCTCT TCTACCTGAA GAGCAAGTCC CCCAAGGAGG AGCTGCTGAA GATGTGGGGG
    GAGGAGCTGA CCAGTGAAGA AAGTGTCTTT GAAGTCTTCG TTCTTTACCT CTCGGGAGAA
    CCAAACCGGA ATGGTCACAA AGTGACTTGC CTGCCCTGGA ACGATGAGCC CCTGGCGGCT
    GAGACCAGCC TGCTGAAGGA GGAGCTGCTG CGGGTGAACC GCCAGGGCAT CCTCACCATC
    AACTCACAGC CCAACATCAA CGGGAAGCCG TCCTCCGACC CCATCGTGGG CTGGGGCCCC
    AGCGGGGGCT ATGTCTTCCA GAAGGCCTAC TTAGAGTTTT TCACTTCCCG CGAGACAGCG
    GAAGCACTTC TGCAAGTGCT GAAGAAGTAC GAGCTCCGGG TTAATTACCA CCTTGTCAAT
    GTGAAGGGTG AAAACATCAC CAATGCCCCT GAACTGCAGC CGAATGCTGT CACTTGGGGC
    ATCTTCCCTG GGCGAGAGAT CATCCAGCCC ACCGTAGTGG ATCCCGTCAG CTTCATGTTC
    TGGAAGGACG AGGCCTTTGC CCTGTGGATT GAGCGGTGGG GAAAGCTGTA TGAGGAGGAG
    TCCCCGTCCC GCACCATCAT CCAGTACATC CACGACAACT ACTTCCTGGT CAACCTGGTG
    GACAATGACT TCCCACTGGA CAACTGCCTC TGGCAGGTGG TGGAAGACAC ATTGGAGCTT
    CTCAACAGGC CCACCCAGAA TGCGAGAGAA ACGGAGGCTC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu33450
    Clone ID Related Accession (Same CDS sequence) XM_005263463.4 , XM_005263463.3 , XM_024447198.1
    Accession Version XM_024447198.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCAGCAG GTGGCCCCCT CTACATAGAC GTGACCTGGC ACCCAGCAGG TGACCCTGGC 
    TCAGACAAGG AGACCTCCTC CATGATGATC GCCAGCACCG CCGTGAACTA CTGTGGCCTG
    GAGACCATCC TGCACATGAC CTGCTGCCGT CAGCGCCTGG AGGAGATCAC GGGCCATCTG
    CACAAAGCTA AGCAGCTGGG CCTGAAGAAC ATCATGGCGC TGCGGGGAGA CCCAATAGGT
    GACCAGTGGG AAGAGGAGGA GGGAGGCTTC AACTACGCAG TGGACCTGGT GAAGCACATC
    CGAAGTGAGT TTGGTGACTA CTTTGACATC TGTGTGGCAG GTTACCCCAA AGGCCACCCC
    GAAGCAGGGA GCTTTGAGGC TGACCTGAAG CACTTGAAGG AGAAGGTGTC TGCGGGAGCC
    GATTTCATCA TCACGCAGCT TTTCTTTGAG GCTGACACAT TCTTCCGCTT TGTGAAGGCA
    TGCACCGACA TGGGCATCAC TTGCCCCATC GTCCCCGGGA TCTTTCCCAT CCAGGGCTAC
    CACTCCCTTC GGCAGCTTGT GAAGCTGTCC AAGCTGGAGG TGCCACAGGA GATCAAGGAC
    GTGATTGAGC CAATCAAAGA CAACGATGCT GCCATCCGCA ACTATGGCAT CGAGCTGGCC
    GTGAGCCTGT GCCAGGAGCT TCTGGCCAGT GGCTTGGTGC CAGGCCTCCA CTTCTACACC
    CTCAACCGCG AGATGGCTAC CACAGAGGTG CTGAAGCGCC TGGGGATGTG GACTGAGGAC
    CCCAGGCGTC CCCTACCCTG GGCTCTCAGC GCCCACCCCA AGCGCCGAGA GGAAGATGTA
    CGTCCCATCT TCTGGGCCTC CAGACCAAAG AGTTACATCT ACCGTACCCA GGAGTGGGAC
    GAGTTCCCTA ACGGCCGCTG GGGCAATTCC TCTTCCCCTG CCTTTGGGGA GCTGAAGGAC
    TACTACCTCT TCTACCTGAA GAGCAAGTCC CCCAAGGAGG AGCTGCTGAA GATGTGGGGG
    GAGGAGCTGA CCAGTGAAGA AAGTGTCTTT GAAGTCTTCG TTCTTTACCT CTCGGGAGAA
    CCAAACCGGA ATGGTCACAA AGTGACTTGC CTGCCCTGGA ACGATGAGCC CCTGGCGGCT
    GAGACCAGCC TGCTGAAGGA GGAGCTGCTG CGGGTGAACC GCCAGGGCAT CCTCACCATC
    AACTCACAGC CCAACATCAA CGGGAAGCCG TCCTCCGACC CCATCGTGGG CTGGGGCCCC
    AGCGGGGGCT ATGTCTTCCA GAAGGCCTAC TTAGAGTTTT TCACTTCCCG CGAGACAGCG
    GAAGCACTTC TGCAAGTGCT GAAGAAGTAC GAGCTCCGGG TTAATTACCA CCTTGTCAAT
    GTGAAGGGTG AAAACATCAC CAATGCCCCT GAACTGCAGC CGAATGCTGT CACTTGGGGC
    ATCTTCCCTG GGCGAGAGAT CATCCAGCCC ACCGTAGTGG ATCCCGTCAG CTTCATGTTC
    TGGAAGGACG AGGCCTTTGC CCTGTGGATT GAGCGGTGGG GAAAGCTGTA TGAGGAGGAG
    TCCCCGTCCC GCACCATCAT CCAGTACATC CACGACAACT ACTTCCTGGT CAACCTGGTG
    GACAATGACT TCCCACTGGA CAACTGCCTC TGGCAGGTGG TGGAAGACAC ATTGGAGCTT
    CTCAACAGGC CCACCCAGAA TGCGAGAGAA ACGGAGGCTC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024302966.1
    CDS399..2123
    Translation

    Target ORF information:

    RefSeq Version XM_024447198.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024447198.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGCAGCAG GTGGCCCCCT CTACATAGAC GTGACCTGGC ACCCAGCAGG TGACCCTGGC 
    TCAGACAAGG AGACCTCCTC CATGATGATC GCCAGCACCG CCGTGAACTA CTGTGGCCTG
    GAGACCATCC TGCACATGAC CTGCTGCCGT CAGCGCCTGG AGGAGATCAC GGGCCATCTG
    CACAAAGCTA AGCAGCTGGG CCTGAAGAAC ATCATGGCGC TGCGGGGAGA CCCAATAGGT
    GACCAGTGGG AAGAGGAGGA GGGAGGCTTC AACTACGCAG TGGACCTGGT GAAGCACATC
    CGAAGTGAGT TTGGTGACTA CTTTGACATC TGTGTGGCAG GTTACCCCAA AGGCCACCCC
    GAAGCAGGGA GCTTTGAGGC TGACCTGAAG CACTTGAAGG AGAAGGTGTC TGCGGGAGCC
    GATTTCATCA TCACGCAGCT TTTCTTTGAG GCTGACACAT TCTTCCGCTT TGTGAAGGCA
    TGCACCGACA TGGGCATCAC TTGCCCCATC GTCCCCGGGA TCTTTCCCAT CCAGGGCTAC
    CACTCCCTTC GGCAGCTTGT GAAGCTGTCC AAGCTGGAGG TGCCACAGGA GATCAAGGAC
    GTGATTGAGC CAATCAAAGA CAACGATGCT GCCATCCGCA ACTATGGCAT CGAGCTGGCC
    GTGAGCCTGT GCCAGGAGCT TCTGGCCAGT GGCTTGGTGC CAGGCCTCCA CTTCTACACC
    CTCAACCGCG AGATGGCTAC CACAGAGGTG CTGAAGCGCC TGGGGATGTG GACTGAGGAC
    CCCAGGCGTC CCCTACCCTG GGCTCTCAGC GCCCACCCCA AGCGCCGAGA GGAAGATGTA
    CGTCCCATCT TCTGGGCCTC CAGACCAAAG AGTTACATCT ACCGTACCCA GGAGTGGGAC
    GAGTTCCCTA ACGGCCGCTG GGGCAATTCC TCTTCCCCTG CCTTTGGGGA GCTGAAGGAC
    TACTACCTCT TCTACCTGAA GAGCAAGTCC CCCAAGGAGG AGCTGCTGAA GATGTGGGGG
    GAGGAGCTGA CCAGTGAAGA AAGTGTCTTT GAAGTCTTCG TTCTTTACCT CTCGGGAGAA
    CCAAACCGGA ATGGTCACAA AGTGACTTGC CTGCCCTGGA ACGATGAGCC CCTGGCGGCT
    GAGACCAGCC TGCTGAAGGA GGAGCTGCTG CGGGTGAACC GCCAGGGCAT CCTCACCATC
    AACTCACAGC CCAACATCAA CGGGAAGCCG TCCTCCGACC CCATCGTGGG CTGGGGCCCC
    AGCGGGGGCT ATGTCTTCCA GAAGGCCTAC TTAGAGTTTT TCACTTCCCG CGAGACAGCG
    GAAGCACTTC TGCAAGTGCT GAAGAAGTAC GAGCTCCGGG TTAATTACCA CCTTGTCAAT
    GTGAAGGGTG AAAACATCAC CAATGCCCCT GAACTGCAGC CGAATGCTGT CACTTGGGGC
    ATCTTCCCTG GGCGAGAGAT CATCCAGCCC ACCGTAGTGG ATCCCGTCAG CTTCATGTTC
    TGGAAGGACG AGGCCTTTGC CCTGTGGATT GAGCGGTGGG GAAAGCTGTA TGAGGAGGAG
    TCCCCGTCCC GCACCATCAT CCAGTACATC CACGACAACT ACTTCCTGGT CAACCTGGTG
    GACAATGACT TCCCACTGGA CAACTGCCTC TGGCAGGTGG TGGAAGACAC ATTGGAGCTT
    CTCAACAGGC CCACCCAGAA TGCGAGAGAA ACGGAGGCTC CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59084
    Clone ID Related Accession (Same CDS sequence) XM_011541495.2 , XM_011541495.3
    Accession Version XM_011541495.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541495.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG GAACCCAGCC
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539797.1
    CDS944..3034
    Translation

    Target ORF information:

    RefSeq Version XM_011541495.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011541495.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG GAACCCAGCC
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59084
    Clone ID Related Accession (Same CDS sequence) XM_011541495.2 , XM_011541495.3
    Accession Version XM_011541495.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2091bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011541495.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG GAACCCAGCC
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539797.1
    CDS748..2838
    Translation

    Target ORF information:

    RefSeq Version XM_011541495.3
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011541495.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG GAACCCAGCC
    ATGGTGAACG AAGCCAGAGG AAACAGCAGC CTCAACCCCT GCTTGGAGGG CAGTGCCAGC
    AGTGGCAGTG AGAGCTCCAA AGATAGTTCG AGATGTTCCA CCCCGGGCCT GGACCCCGAG
    CGGCATGAGA GACTCCGGGA GAAGATGAGG CGGCGATTGG AATCTGGTGA CAAGTGGTTC
    TCCCTGGAAT TCTTCCCTCC TCGAACTGCT GAGGGAGCTG TCAATCTCAT CTCAAGGTTT
    GACCGGATGG CAGCAGGTGG CCCCCTCTAC ATAGACGTGA CCTGGCACCC AGCAGGTGAC
    CCTGGCTCAG ACAAGGAGAC CTCCTCCATG ATGATCGCCA GCACCGCCGT GAACTACTGT
    GGCCTGGAGA CCATCCTGCA CATGACCTGC TGCCGTCAGC GCCTGGAGGA GATCACGGGC
    CATCTGCACA AAGCTAAGCA GCTGGGCCTG AAGAACATCA TGGCGCTGCG GGGAGACCCA
    ATAGGTGACC AGTGGGAAGA GGAGGAGGGA GGCTTCAACT ACGCAGTGGA CCTGGTGAAG
    CACATCCGAA GTGAGTTTGG TGACTACTTT GACATCTGTG TGGCAGGTTA CCCCAAAGGC
    CACCCCGAAG CAGGGAGCTT TGAGGCTGAC CTGAAGCACT TGAAGGAGAA GGTGTCTGCG
    GGAGCCGATT TCATCATCAC GCAGCTTTTC TTTGAGGCTG ACACATTCTT CCGCTTTGTG
    AAGGCATGCA CCGACATGGG CATCACTTGC CCCATCGTCC CCGGGATCTT TCCCATCCAG
    GGCTACCACT CCCTTCGGCA GCTTGTGAAG CTGTCCAAGC TGGAGGTGCC ACAGGAGATC
    AAGGACGTGA TTGAGCCAAT CAAAGACAAC GATGCTGCCA TCCGCAACTA TGGCATCGAG
    CTGGCCGTGA GCCTGTGCCA GGAGCTTCTG GCCAGTGGCT TGGTGCCAGG CCTCCACTTC
    TACACCCTCA ACCGCGAGAT GGCTACCACA GAGGTGCTGA AGCGCCTGGG GATGTGGACT
    GAGGACCCCA GGCGTCCCCT ACCCTGGGCT CTCAGCGCCC ACCCCAAGCG CCGAGAGGAA
    GATGTACGTC CCATCTTCTG GGCCTCCAGA CCAAAGAGTT ACATCTACCG TACCCAGGAG
    TGGGACGAGT TCCCTAACGG CCGCTGGGGC AATTCCTCTT CCCCTGCCTT TGGGGAGCTG
    AAGGACTACT ACCTCTTCTA CCTGAAGAGC AAGTCCCCCA AGGAGGAGCT GCTGAAGATG
    TGGGGGGAGG AGCTGACCAG TGAAGAAAGT GTCTTTGAAG TCTTCGTTCT TTACCTCTCG
    GGAGAACCAA ACCGGAATGG TCACAAAGTG ACTTGCCTGC CCTGGAACGA TGAGCCCCTG
    GCGGCTGAGA CCAGCCTGCT GAAGGAGGAG CTGCTGCGGG TGAACCGCCA GGGCATCCTC
    ACCATCAACT CACAGCCCAA CATCAACGGG AAGCCGTCCT CCGACCCCAT CGTGGGCTGG
    GGCCCCAGCG GGGGCTATGT CTTCCAGAAG GCCTACTTAG AGTTTTTCAC TTCCCGCGAG
    ACAGCGGAAG CACTTCTGCA AGTGCTGAAG AAGTACGAGC TCCGGGTTAA TTACCACCTT
    GTCAATGTGA AGGGTGAAAA CATCACCAAT GCCCCTGAAC TGCAGCCGAA TGCTGTCACT
    TGGGGCATCT TCCCTGGGCG AGAGATCATC CAGCCCACCG TAGTGGATCC CGTCAGCTTC
    ATGTTCTGGA AGGACGAGGC CTTTGCCCTG TGGATTGAGC GGTGGGGAAA GCTGTATGAG
    GAGGAGTCCC CGTCCCGCAC CATCATCCAG TACATCCACG ACAACTACTT CCTGGTCAAC
    CTGGTGGACA ATGACTTCCC ACTGGACAAC TGCCTCTGGC AGGTGGTGGA AGACACATTG
    GAGCTTCTCA ACAGGCCCAC CCAGAATGCG AGAGAAACGG AGGCTCCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59085
    Clone ID Related Accession (Same CDS sequence) XM_011541496.3 , XM_011541496.2
    Accession Version XM_011541496.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011541496.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG TAGGAACCCA
    GCCATGGTGA ACGAAGCCAG AGGAAACAGC AGCCTCAACC CCTGCTTGGA GGGCAGTGCC
    AGCAGTGGCA GTGAGAGCTC CAAAGATAGT TCGAGATGTT CCACCCCGGG CCTGGACCCC
    GAGCGGCATG AGAGACTCCG GGAGAAGATG AGGCGGCGAT TGGAATCTGG TGACAAGTGG
    TTCTCCCTGG AATTCTTCCC TCCTCGAACT GCTGAGGGAG CTGTCAATCT CATCTCAAGG
    TTTGACCGGA TGGCAGCAGG TGGCCCCCTC TACATAGACG TGACCTGGCA CCCAGCAGGT
    GACCCTGGCT CAGACAAGGA GACCTCCTCC ATGATGATCG CCAGCACCGC CGTGAACTAC
    TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCCGTC AGCGCCTGGA GGAGATCACG
    GGCCATCTGC ACAAAGCTAA GCAGCTGGGC CTGAAGAACA TCATGGCGCT GCGGGGAGAC
    CCAATAGGTG ACCAGTGGGA AGAGGAGGAG GGAGGCTTCA ACTACGCAGT GGACCTGGTG
    AAGCACATCC GAAGTGAGTT TGGTGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA
    GGCCACCCCG AAGCAGGGAG CTTTGAGGCT GACCTGAAGC ACTTGAAGGA GAAGGTGTCT
    GCGGGAGCCG ATTTCATCAT CACGCAGCTT TTCTTTGAGG CTGACACATT CTTCCGCTTT
    GTGAAGGCAT GCACCGACAT GGGCATCACT TGCCCCATCG TCCCCGGGAT CTTTCCCATC
    CAGGGCTACC ACTCCCTTCG GCAGCTTGTG AAGCTGTCCA AGCTGGAGGT GCCACAGGAG
    ATCAAGGACG TGATTGAGCC AATCAAAGAC AACGATGCTG CCATCCGCAA CTATGGCATC
    GAGCTGGCCG TGAGCCTGTG CCAGGAGCTT CTGGCCAGTG GCTTGGTGCC AGGCCTCCAC
    TTCTACACCC TCAACCGCGA GATGGCTACC ACAGAGGTGC TGAAGCGCCT GGGGATGTGG
    ACTGAGGACC CCAGGCGTCC CCTACCCTGG GCTCTCAGCG CCCACCCCAA GCGCCGAGAG
    GAAGATGTAC GTCCCATCTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACCCAG
    GAGTGGGACG AGTTCCCTAA CGGCCGCTGG GGCAATTCCT CTTCCCCTGC CTTTGGGGAG
    CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CCAAGGAGGA GCTGCTGAAG
    ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGTGTCTTTG AAGTCTTCGT TCTTTACCTC
    TCGGGAGAAC CAAACCGGAA TGGTCACAAA GTGACTTGCC TGCCCTGGAA CGATGAGCCC
    CTGGCGGCTG AGACCAGCCT GCTGAAGGAG GAGCTGCTGC GGGTGAACCG CCAGGGCATC
    CTCACCATCA ACTCACAGCC CAACATCAAC GGGAAGCCGT CCTCCGACCC CATCGTGGGC
    TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TAGAGTTTTT CACTTCCCGC
    GAGACAGCGG AAGCACTTCT GCAAGTGCTG AAGAAGTACG AGCTCCGGGT TAATTACCAC
    CTTGTCAATG TGAAGGGTGA AAACATCACC AATGCCCCTG AACTGCAGCC GAATGCTGTC
    ACTTGGGGCA TCTTCCCTGG GCGAGAGATC ATCCAGCCCA CCGTAGTGGA TCCCGTCAGC
    TTCATGTTCT GGAAGGCCCA CCCAGAATGC GAGAGAAACG GAGGCTCCAT GACCCTGCGT
    CCTGACGCCC TGCGTTGGAG CCACTCCTGT CCCGCCTTCC TCCTCCACAG TGCTGCTTCT
    CTTGGGAACT CCACTCTCCT TCGTGTCTCT CCCACCCCGG CCTCCACTCC CCCACCTGAC
    AATGGCAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539798.1
    CDS24..2075
    Translation

    Target ORF information:

    RefSeq Version XM_011541496.3
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011541496.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG TAGGAACCCA
    GCCATGGTGA ACGAAGCCAG AGGAAACAGC AGCCTCAACC CCTGCTTGGA GGGCAGTGCC
    AGCAGTGGCA GTGAGAGCTC CAAAGATAGT TCGAGATGTT CCACCCCGGG CCTGGACCCC
    GAGCGGCATG AGAGACTCCG GGAGAAGATG AGGCGGCGAT TGGAATCTGG TGACAAGTGG
    TTCTCCCTGG AATTCTTCCC TCCTCGAACT GCTGAGGGAG CTGTCAATCT CATCTCAAGG
    TTTGACCGGA TGGCAGCAGG TGGCCCCCTC TACATAGACG TGACCTGGCA CCCAGCAGGT
    GACCCTGGCT CAGACAAGGA GACCTCCTCC ATGATGATCG CCAGCACCGC CGTGAACTAC
    TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCCGTC AGCGCCTGGA GGAGATCACG
    GGCCATCTGC ACAAAGCTAA GCAGCTGGGC CTGAAGAACA TCATGGCGCT GCGGGGAGAC
    CCAATAGGTG ACCAGTGGGA AGAGGAGGAG GGAGGCTTCA ACTACGCAGT GGACCTGGTG
    AAGCACATCC GAAGTGAGTT TGGTGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA
    GGCCACCCCG AAGCAGGGAG CTTTGAGGCT GACCTGAAGC ACTTGAAGGA GAAGGTGTCT
    GCGGGAGCCG ATTTCATCAT CACGCAGCTT TTCTTTGAGG CTGACACATT CTTCCGCTTT
    GTGAAGGCAT GCACCGACAT GGGCATCACT TGCCCCATCG TCCCCGGGAT CTTTCCCATC
    CAGGGCTACC ACTCCCTTCG GCAGCTTGTG AAGCTGTCCA AGCTGGAGGT GCCACAGGAG
    ATCAAGGACG TGATTGAGCC AATCAAAGAC AACGATGCTG CCATCCGCAA CTATGGCATC
    GAGCTGGCCG TGAGCCTGTG CCAGGAGCTT CTGGCCAGTG GCTTGGTGCC AGGCCTCCAC
    TTCTACACCC TCAACCGCGA GATGGCTACC ACAGAGGTGC TGAAGCGCCT GGGGATGTGG
    ACTGAGGACC CCAGGCGTCC CCTACCCTGG GCTCTCAGCG CCCACCCCAA GCGCCGAGAG
    GAAGATGTAC GTCCCATCTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACCCAG
    GAGTGGGACG AGTTCCCTAA CGGCCGCTGG GGCAATTCCT CTTCCCCTGC CTTTGGGGAG
    CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CCAAGGAGGA GCTGCTGAAG
    ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGTGTCTTTG AAGTCTTCGT TCTTTACCTC
    TCGGGAGAAC CAAACCGGAA TGGTCACAAA GTGACTTGCC TGCCCTGGAA CGATGAGCCC
    CTGGCGGCTG AGACCAGCCT GCTGAAGGAG GAGCTGCTGC GGGTGAACCG CCAGGGCATC
    CTCACCATCA ACTCACAGCC CAACATCAAC GGGAAGCCGT CCTCCGACCC CATCGTGGGC
    TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TAGAGTTTTT CACTTCCCGC
    GAGACAGCGG AAGCACTTCT GCAAGTGCTG AAGAAGTACG AGCTCCGGGT TAATTACCAC
    CTTGTCAATG TGAAGGGTGA AAACATCACC AATGCCCCTG AACTGCAGCC GAATGCTGTC
    ACTTGGGGCA TCTTCCCTGG GCGAGAGATC ATCCAGCCCA CCGTAGTGGA TCCCGTCAGC
    TTCATGTTCT GGAAGGCCCA CCCAGAATGC GAGAGAAACG GAGGCTCCAT GACCCTGCGT
    CCTGACGCCC TGCGTTGGAG CCACTCCTGT CCCGCCTTCC TCCTCCACAG TGCTGCTTCT
    CTTGGGAACT CCACTCTCCT TCGTGTCTCT CCCACCCCGG CCTCCACTCC CCCACCTGAC
    AATGGCAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59085
    Clone ID Related Accession (Same CDS sequence) XM_011541496.3 , XM_011541496.2
    Accession Version XM_011541496.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2052bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541496.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG TAGGAACCCA
    GCCATGGTGA ACGAAGCCAG AGGAAACAGC AGCCTCAACC CCTGCTTGGA GGGCAGTGCC
    AGCAGTGGCA GTGAGAGCTC CAAAGATAGT TCGAGATGTT CCACCCCGGG CCTGGACCCC
    GAGCGGCATG AGAGACTCCG GGAGAAGATG AGGCGGCGAT TGGAATCTGG TGACAAGTGG
    TTCTCCCTGG AATTCTTCCC TCCTCGAACT GCTGAGGGAG CTGTCAATCT CATCTCAAGG
    TTTGACCGGA TGGCAGCAGG TGGCCCCCTC TACATAGACG TGACCTGGCA CCCAGCAGGT
    GACCCTGGCT CAGACAAGGA GACCTCCTCC ATGATGATCG CCAGCACCGC CGTGAACTAC
    TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCCGTC AGCGCCTGGA GGAGATCACG
    GGCCATCTGC ACAAAGCTAA GCAGCTGGGC CTGAAGAACA TCATGGCGCT GCGGGGAGAC
    CCAATAGGTG ACCAGTGGGA AGAGGAGGAG GGAGGCTTCA ACTACGCAGT GGACCTGGTG
    AAGCACATCC GAAGTGAGTT TGGTGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA
    GGCCACCCCG AAGCAGGGAG CTTTGAGGCT GACCTGAAGC ACTTGAAGGA GAAGGTGTCT
    GCGGGAGCCG ATTTCATCAT CACGCAGCTT TTCTTTGAGG CTGACACATT CTTCCGCTTT
    GTGAAGGCAT GCACCGACAT GGGCATCACT TGCCCCATCG TCCCCGGGAT CTTTCCCATC
    CAGGGCTACC ACTCCCTTCG GCAGCTTGTG AAGCTGTCCA AGCTGGAGGT GCCACAGGAG
    ATCAAGGACG TGATTGAGCC AATCAAAGAC AACGATGCTG CCATCCGCAA CTATGGCATC
    GAGCTGGCCG TGAGCCTGTG CCAGGAGCTT CTGGCCAGTG GCTTGGTGCC AGGCCTCCAC
    TTCTACACCC TCAACCGCGA GATGGCTACC ACAGAGGTGC TGAAGCGCCT GGGGATGTGG
    ACTGAGGACC CCAGGCGTCC CCTACCCTGG GCTCTCAGCG CCCACCCCAA GCGCCGAGAG
    GAAGATGTAC GTCCCATCTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACCCAG
    GAGTGGGACG AGTTCCCTAA CGGCCGCTGG GGCAATTCCT CTTCCCCTGC CTTTGGGGAG
    CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CCAAGGAGGA GCTGCTGAAG
    ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGTGTCTTTG AAGTCTTCGT TCTTTACCTC
    TCGGGAGAAC CAAACCGGAA TGGTCACAAA GTGACTTGCC TGCCCTGGAA CGATGAGCCC
    CTGGCGGCTG AGACCAGCCT GCTGAAGGAG GAGCTGCTGC GGGTGAACCG CCAGGGCATC
    CTCACCATCA ACTCACAGCC CAACATCAAC GGGAAGCCGT CCTCCGACCC CATCGTGGGC
    TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TAGAGTTTTT CACTTCCCGC
    GAGACAGCGG AAGCACTTCT GCAAGTGCTG AAGAAGTACG AGCTCCGGGT TAATTACCAC
    CTTGTCAATG TGAAGGGTGA AAACATCACC AATGCCCCTG AACTGCAGCC GAATGCTGTC
    ACTTGGGGCA TCTTCCCTGG GCGAGAGATC ATCCAGCCCA CCGTAGTGGA TCCCGTCAGC
    TTCATGTTCT GGAAGGCCCA CCCAGAATGC GAGAGAAACG GAGGCTCCAT GACCCTGCGT
    CCTGACGCCC TGCGTTGGAG CCACTCCTGT CCCGCCTTCC TCCTCCACAG TGCTGCTTCT
    CTTGGGAACT CCACTCTCCT TCGTGTCTCT CCCACCCCGG CCTCCACTCC CCCACCTGAC
    AATGGCAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539798.1
    CDS944..2995
    Translation

    Target ORF information:

    RefSeq Version XM_011541496.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011541496.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG TAGGAACCCA
    GCCATGGTGA ACGAAGCCAG AGGAAACAGC AGCCTCAACC CCTGCTTGGA GGGCAGTGCC
    AGCAGTGGCA GTGAGAGCTC CAAAGATAGT TCGAGATGTT CCACCCCGGG CCTGGACCCC
    GAGCGGCATG AGAGACTCCG GGAGAAGATG AGGCGGCGAT TGGAATCTGG TGACAAGTGG
    TTCTCCCTGG AATTCTTCCC TCCTCGAACT GCTGAGGGAG CTGTCAATCT CATCTCAAGG
    TTTGACCGGA TGGCAGCAGG TGGCCCCCTC TACATAGACG TGACCTGGCA CCCAGCAGGT
    GACCCTGGCT CAGACAAGGA GACCTCCTCC ATGATGATCG CCAGCACCGC CGTGAACTAC
    TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCCGTC AGCGCCTGGA GGAGATCACG
    GGCCATCTGC ACAAAGCTAA GCAGCTGGGC CTGAAGAACA TCATGGCGCT GCGGGGAGAC
    CCAATAGGTG ACCAGTGGGA AGAGGAGGAG GGAGGCTTCA ACTACGCAGT GGACCTGGTG
    AAGCACATCC GAAGTGAGTT TGGTGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA
    GGCCACCCCG AAGCAGGGAG CTTTGAGGCT GACCTGAAGC ACTTGAAGGA GAAGGTGTCT
    GCGGGAGCCG ATTTCATCAT CACGCAGCTT TTCTTTGAGG CTGACACATT CTTCCGCTTT
    GTGAAGGCAT GCACCGACAT GGGCATCACT TGCCCCATCG TCCCCGGGAT CTTTCCCATC
    CAGGGCTACC ACTCCCTTCG GCAGCTTGTG AAGCTGTCCA AGCTGGAGGT GCCACAGGAG
    ATCAAGGACG TGATTGAGCC AATCAAAGAC AACGATGCTG CCATCCGCAA CTATGGCATC
    GAGCTGGCCG TGAGCCTGTG CCAGGAGCTT CTGGCCAGTG GCTTGGTGCC AGGCCTCCAC
    TTCTACACCC TCAACCGCGA GATGGCTACC ACAGAGGTGC TGAAGCGCCT GGGGATGTGG
    ACTGAGGACC CCAGGCGTCC CCTACCCTGG GCTCTCAGCG CCCACCCCAA GCGCCGAGAG
    GAAGATGTAC GTCCCATCTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACCCAG
    GAGTGGGACG AGTTCCCTAA CGGCCGCTGG GGCAATTCCT CTTCCCCTGC CTTTGGGGAG
    CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CCAAGGAGGA GCTGCTGAAG
    ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGTGTCTTTG AAGTCTTCGT TCTTTACCTC
    TCGGGAGAAC CAAACCGGAA TGGTCACAAA GTGACTTGCC TGCCCTGGAA CGATGAGCCC
    CTGGCGGCTG AGACCAGCCT GCTGAAGGAG GAGCTGCTGC GGGTGAACCG CCAGGGCATC
    CTCACCATCA ACTCACAGCC CAACATCAAC GGGAAGCCGT CCTCCGACCC CATCGTGGGC
    TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TAGAGTTTTT CACTTCCCGC
    GAGACAGCGG AAGCACTTCT GCAAGTGCTG AAGAAGTACG AGCTCCGGGT TAATTACCAC
    CTTGTCAATG TGAAGGGTGA AAACATCACC AATGCCCCTG AACTGCAGCC GAATGCTGTC
    ACTTGGGGCA TCTTCCCTGG GCGAGAGATC ATCCAGCCCA CCGTAGTGGA TCCCGTCAGC
    TTCATGTTCT GGAAGGCCCA CCCAGAATGC GAGAGAAACG GAGGCTCCAT GACCCTGCGT
    CCTGACGCCC TGCGTTGGAG CCACTCCTGT CCCGCCTTCC TCCTCCACAG TGCTGCTTCT
    CTTGGGAACT CCACTCTCCT TCGTGTCTCT CCCACCCCGG CCTCCACTCC CCCACCTGAC
    AATGGCAGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90042
    Clone ID Related Accession (Same CDS sequence) XM_017001328.2 , XM_017001328.1
    Accession Version XM_017001328.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017001328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG TAGGAACCCA
    GCCATGGTGA ACGAAGCCAG AGGAAACAGC AGCCTCAACC CCTGCTTGGA GGGCAGTGCC
    AGCAGTGGCA GTGAGAGCTC CAAAGATAGT TCGAGATGTT CCACCCCGGG CCTGGACCCC
    GAGCGGCATG AGAGACTCCG GGAGAAGATG AGGCGGCGAT TGGAATCTGG TGACAAGTGG
    TTCTCCCTGG AATTCTTCCC TCCTCGAACT GCTGAGGGAG CTGTCAATCT CATCTCAAGG
    TTTGACCGGA TGGCAGCAGG TGGCCCCCTC TACATAGACG TGACCTGGCA CCCAGCAGGT
    GACCCTGGCT CAGACAAGGA GACCTCCTCC ATGATGATCG CCAGCACCGC CGTGAACTAC
    TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCCGTC AGCGCCTGGA GGAGATCACG
    GGCCATCTGC ACAAAGCTAA GCAGCTGGGC CTGAAGAACA TCATGGCGCT GCGGGGAGAC
    CCAATAGGTG ACCAGTGGGA AGAGGAGGAG GGAGGCTTCA ACTACGCAGT GGACCTGGTG
    AAGCACATCC GAAGTGAGTT TGGTGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA
    GGCCACCCCG AAGCAGGGAG CTTTGAGGCT GACCTGAAGC ACTTGAAGGA GAAGGTGTCT
    GCGGGAGCCG ATTTCATCAT CACGCAGCTT TTCTTTGAGG CTGACACATT CTTCCGCTTT
    GTGAAGGCAT GCACCGACAT GGGCATCACT TGCCCCATCG TCCCCGGGAT CTTTCCCATC
    CAGGGCTACC ACTCCCTTCG GCAGCTTGTG AAGCTGTCCA AGCTGGAGGT GCCACAGGAG
    ATCAAGGACG TGATTGAGCC AATCAAAGAC AACGATGCTG CCATCCGCAA CTATGGCATC
    GAGCTGGCCG TGAGCCTGTG CCAGGAGCTT CTGGCCAGTG GCTTGGTGCC AGGCCTCCAC
    TTCTACACCC TCAACCGCGA GATGGCTACC ACAGAGGTGC TGAAGCGCCT GGGGATGTGG
    ACTGAGGACC CCAGGCGTCC CCTACCCTGG GCTCTCAGCG CCCACCCCAA GCGCCGAGAG
    GAAGATGTAC GTCCCATCTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACCCAG
    GAGTGGGACG AGTTCCCTAA CGGCCGCTGG GGCAATTCCT CTTCCCCTGC CTTTGGGGAG
    CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CCAAGGAGGA GCTGCTGAAG
    ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGTGTCTTTG AAGTCTTCGT TCTTTACCTC
    TCGGGAGAAC CAAACCGGAA TGGTCACAAA GTGACTTGCC TGCCCTGGAA CGATGAGCCC
    CTGGCGGCTG AGACCAGCCT GCTGAAGGAG GAGCTGCTGC GGGTGAACCG CCAGGGCATC
    CTCACCATCA ACTCACAGCC CAACATCAAC GGGAAGCCGT CCTCCGACCC CATCGTGGGC
    TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TAGAGTTTTT CACTTCCCGC
    GAGACAGCGG AAGCACTTCT GCAAGTGCTG AAGAAGTACG AGCTCCGGGT TAATTACCAC
    CTTGTCAATG TGAAGGGTGA AAACATCACC AATGCCCCTG AACTGCAGCC GAATGCTGTC
    ACTTGGGGCA TCTTCCCTGG GCGAGAGATC ATCCAGCCCA CCGTAGTGGA TCCCGTCAGC
    TTCATGTTCT GGAAGGTGGT GGAAGACACA TTGGAGCTTC TCAACAGGCC CACCCAGAAT
    GCGAGAGAAA CGGAGGCTCC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016856817.1
    CDS24..1967
    Translation

    Target ORF information:

    RefSeq Version XM_017001328.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (MTHFR), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017001328.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG TAGGAACCCA
    GCCATGGTGA ACGAAGCCAG AGGAAACAGC AGCCTCAACC CCTGCTTGGA GGGCAGTGCC
    AGCAGTGGCA GTGAGAGCTC CAAAGATAGT TCGAGATGTT CCACCCCGGG CCTGGACCCC
    GAGCGGCATG AGAGACTCCG GGAGAAGATG AGGCGGCGAT TGGAATCTGG TGACAAGTGG
    TTCTCCCTGG AATTCTTCCC TCCTCGAACT GCTGAGGGAG CTGTCAATCT CATCTCAAGG
    TTTGACCGGA TGGCAGCAGG TGGCCCCCTC TACATAGACG TGACCTGGCA CCCAGCAGGT
    GACCCTGGCT CAGACAAGGA GACCTCCTCC ATGATGATCG CCAGCACCGC CGTGAACTAC
    TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCCGTC AGCGCCTGGA GGAGATCACG
    GGCCATCTGC ACAAAGCTAA GCAGCTGGGC CTGAAGAACA TCATGGCGCT GCGGGGAGAC
    CCAATAGGTG ACCAGTGGGA AGAGGAGGAG GGAGGCTTCA ACTACGCAGT GGACCTGGTG
    AAGCACATCC GAAGTGAGTT TGGTGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA
    GGCCACCCCG AAGCAGGGAG CTTTGAGGCT GACCTGAAGC ACTTGAAGGA GAAGGTGTCT
    GCGGGAGCCG ATTTCATCAT CACGCAGCTT TTCTTTGAGG CTGACACATT CTTCCGCTTT
    GTGAAGGCAT GCACCGACAT GGGCATCACT TGCCCCATCG TCCCCGGGAT CTTTCCCATC
    CAGGGCTACC ACTCCCTTCG GCAGCTTGTG AAGCTGTCCA AGCTGGAGGT GCCACAGGAG
    ATCAAGGACG TGATTGAGCC AATCAAAGAC AACGATGCTG CCATCCGCAA CTATGGCATC
    GAGCTGGCCG TGAGCCTGTG CCAGGAGCTT CTGGCCAGTG GCTTGGTGCC AGGCCTCCAC
    TTCTACACCC TCAACCGCGA GATGGCTACC ACAGAGGTGC TGAAGCGCCT GGGGATGTGG
    ACTGAGGACC CCAGGCGTCC CCTACCCTGG GCTCTCAGCG CCCACCCCAA GCGCCGAGAG
    GAAGATGTAC GTCCCATCTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACCCAG
    GAGTGGGACG AGTTCCCTAA CGGCCGCTGG GGCAATTCCT CTTCCCCTGC CTTTGGGGAG
    CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CCAAGGAGGA GCTGCTGAAG
    ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGTGTCTTTG AAGTCTTCGT TCTTTACCTC
    TCGGGAGAAC CAAACCGGAA TGGTCACAAA GTGACTTGCC TGCCCTGGAA CGATGAGCCC
    CTGGCGGCTG AGACCAGCCT GCTGAAGGAG GAGCTGCTGC GGGTGAACCG CCAGGGCATC
    CTCACCATCA ACTCACAGCC CAACATCAAC GGGAAGCCGT CCTCCGACCC CATCGTGGGC
    TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TAGAGTTTTT CACTTCCCGC
    GAGACAGCGG AAGCACTTCT GCAAGTGCTG AAGAAGTACG AGCTCCGGGT TAATTACCAC
    CTTGTCAATG TGAAGGGTGA AAACATCACC AATGCCCCTG AACTGCAGCC GAATGCTGTC
    ACTTGGGGCA TCTTCCCTGG GCGAGAGATC ATCCAGCCCA CCGTAGTGGA TCCCGTCAGC
    TTCATGTTCT GGAAGGTGGT GGAAGACACA TTGGAGCTTC TCAACAGGCC CACCCAGAAT
    GCGAGAGAAA CGGAGGCTCC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90042
    Clone ID Related Accession (Same CDS sequence) XM_017001328.2 , XM_017001328.1
    Accession Version XM_017001328.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1944bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product methylenetetrahydrofolate reductase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG TAGGAACCCA
    GCCATGGTGA ACGAAGCCAG AGGAAACAGC AGCCTCAACC CCTGCTTGGA GGGCAGTGCC
    AGCAGTGGCA GTGAGAGCTC CAAAGATAGT TCGAGATGTT CCACCCCGGG CCTGGACCCC
    GAGCGGCATG AGAGACTCCG GGAGAAGATG AGGCGGCGAT TGGAATCTGG TGACAAGTGG
    TTCTCCCTGG AATTCTTCCC TCCTCGAACT GCTGAGGGAG CTGTCAATCT CATCTCAAGG
    TTTGACCGGA TGGCAGCAGG TGGCCCCCTC TACATAGACG TGACCTGGCA CCCAGCAGGT
    GACCCTGGCT CAGACAAGGA GACCTCCTCC ATGATGATCG CCAGCACCGC CGTGAACTAC
    TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCCGTC AGCGCCTGGA GGAGATCACG
    GGCCATCTGC ACAAAGCTAA GCAGCTGGGC CTGAAGAACA TCATGGCGCT GCGGGGAGAC
    CCAATAGGTG ACCAGTGGGA AGAGGAGGAG GGAGGCTTCA ACTACGCAGT GGACCTGGTG
    AAGCACATCC GAAGTGAGTT TGGTGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA
    GGCCACCCCG AAGCAGGGAG CTTTGAGGCT GACCTGAAGC ACTTGAAGGA GAAGGTGTCT
    GCGGGAGCCG ATTTCATCAT CACGCAGCTT TTCTTTGAGG CTGACACATT CTTCCGCTTT
    GTGAAGGCAT GCACCGACAT GGGCATCACT TGCCCCATCG TCCCCGGGAT CTTTCCCATC
    CAGGGCTACC ACTCCCTTCG GCAGCTTGTG AAGCTGTCCA AGCTGGAGGT GCCACAGGAG
    ATCAAGGACG TGATTGAGCC AATCAAAGAC AACGATGCTG CCATCCGCAA CTATGGCATC
    GAGCTGGCCG TGAGCCTGTG CCAGGAGCTT CTGGCCAGTG GCTTGGTGCC AGGCCTCCAC
    TTCTACACCC TCAACCGCGA GATGGCTACC ACAGAGGTGC TGAAGCGCCT GGGGATGTGG
    ACTGAGGACC CCAGGCGTCC CCTACCCTGG GCTCTCAGCG CCCACCCCAA GCGCCGAGAG
    GAAGATGTAC GTCCCATCTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACCCAG
    GAGTGGGACG AGTTCCCTAA CGGCCGCTGG GGCAATTCCT CTTCCCCTGC CTTTGGGGAG
    CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CCAAGGAGGA GCTGCTGAAG
    ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGTGTCTTTG AAGTCTTCGT TCTTTACCTC
    TCGGGAGAAC CAAACCGGAA TGGTCACAAA GTGACTTGCC TGCCCTGGAA CGATGAGCCC
    CTGGCGGCTG AGACCAGCCT GCTGAAGGAG GAGCTGCTGC GGGTGAACCG CCAGGGCATC
    CTCACCATCA ACTCACAGCC CAACATCAAC GGGAAGCCGT CCTCCGACCC CATCGTGGGC
    TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TAGAGTTTTT CACTTCCCGC
    GAGACAGCGG AAGCACTTCT GCAAGTGCTG AAGAAGTACG AGCTCCGGGT TAATTACCAC
    CTTGTCAATG TGAAGGGTGA AAACATCACC AATGCCCCTG AACTGCAGCC GAATGCTGTC
    ACTTGGGGCA TCTTCCCTGG GCGAGAGATC ATCCAGCCCA CCGTAGTGGA TCCCGTCAGC
    TTCATGTTCT GGAAGGTGGT GGAAGACACA TTGGAGCTTC TCAACAGGCC CACCCAGAAT
    GCGAGAGAAA CGGAGGCTCC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016856817.1
    CDS944..2887
    Translation

    Target ORF information:

    RefSeq Version XM_017001328.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methylenetetrahydrofolate reductase (NAD(P)H) (MTHFR), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017001328.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGACCATC GAAAAGCCAG GGTCCTCCCA GCTGGGCACT ACTGCCCCTC GCTAGGAATA 
    TGGGCCTCGC AGGTCGGCAG CGTGAGGTCC TCTGTGCCAC CTTCCATCAG TAGGAACCCA
    GCCATGGTGA ACGAAGCCAG AGGAAACAGC AGCCTCAACC CCTGCTTGGA GGGCAGTGCC
    AGCAGTGGCA GTGAGAGCTC CAAAGATAGT TCGAGATGTT CCACCCCGGG CCTGGACCCC
    GAGCGGCATG AGAGACTCCG GGAGAAGATG AGGCGGCGAT TGGAATCTGG TGACAAGTGG
    TTCTCCCTGG AATTCTTCCC TCCTCGAACT GCTGAGGGAG CTGTCAATCT CATCTCAAGG
    TTTGACCGGA TGGCAGCAGG TGGCCCCCTC TACATAGACG TGACCTGGCA CCCAGCAGGT
    GACCCTGGCT CAGACAAGGA GACCTCCTCC ATGATGATCG CCAGCACCGC CGTGAACTAC
    TGTGGCCTGG AGACCATCCT GCACATGACC TGCTGCCGTC AGCGCCTGGA GGAGATCACG
    GGCCATCTGC ACAAAGCTAA GCAGCTGGGC CTGAAGAACA TCATGGCGCT GCGGGGAGAC
    CCAATAGGTG ACCAGTGGGA AGAGGAGGAG GGAGGCTTCA ACTACGCAGT GGACCTGGTG
    AAGCACATCC GAAGTGAGTT TGGTGACTAC TTTGACATCT GTGTGGCAGG TTACCCCAAA
    GGCCACCCCG AAGCAGGGAG CTTTGAGGCT GACCTGAAGC ACTTGAAGGA GAAGGTGTCT
    GCGGGAGCCG ATTTCATCAT CACGCAGCTT TTCTTTGAGG CTGACACATT CTTCCGCTTT
    GTGAAGGCAT GCACCGACAT GGGCATCACT TGCCCCATCG TCCCCGGGAT CTTTCCCATC
    CAGGGCTACC ACTCCCTTCG GCAGCTTGTG AAGCTGTCCA AGCTGGAGGT GCCACAGGAG
    ATCAAGGACG TGATTGAGCC AATCAAAGAC AACGATGCTG CCATCCGCAA CTATGGCATC
    GAGCTGGCCG TGAGCCTGTG CCAGGAGCTT CTGGCCAGTG GCTTGGTGCC AGGCCTCCAC
    TTCTACACCC TCAACCGCGA GATGGCTACC ACAGAGGTGC TGAAGCGCCT GGGGATGTGG
    ACTGAGGACC CCAGGCGTCC CCTACCCTGG GCTCTCAGCG CCCACCCCAA GCGCCGAGAG
    GAAGATGTAC GTCCCATCTT CTGGGCCTCC AGACCAAAGA GTTACATCTA CCGTACCCAG
    GAGTGGGACG AGTTCCCTAA CGGCCGCTGG GGCAATTCCT CTTCCCCTGC CTTTGGGGAG
    CTGAAGGACT ACTACCTCTT CTACCTGAAG AGCAAGTCCC CCAAGGAGGA GCTGCTGAAG
    ATGTGGGGGG AGGAGCTGAC CAGTGAAGAA AGTGTCTTTG AAGTCTTCGT TCTTTACCTC
    TCGGGAGAAC CAAACCGGAA TGGTCACAAA GTGACTTGCC TGCCCTGGAA CGATGAGCCC
    CTGGCGGCTG AGACCAGCCT GCTGAAGGAG GAGCTGCTGC GGGTGAACCG CCAGGGCATC
    CTCACCATCA ACTCACAGCC CAACATCAAC GGGAAGCCGT CCTCCGACCC CATCGTGGGC
    TGGGGCCCCA GCGGGGGCTA TGTCTTCCAG AAGGCCTACT TAGAGTTTTT CACTTCCCGC
    GAGACAGCGG AAGCACTTCT GCAAGTGCTG AAGAAGTACG AGCTCCGGGT TAATTACCAC
    CTTGTCAATG TGAAGGGTGA AAACATCACC AATGCCCCTG AACTGCAGCC GAATGCTGTC
    ACTTGGGGCA TCTTCCCTGG GCGAGAGATC ATCCAGCCCA CCGTAGTGGA TCCCGTCAGC
    TTCATGTTCT GGAAGGTGGT GGAAGACACA TTGGAGCTTC TCAACAGGCC CACCCAGAAT
    GCGAGAGAAA CGGAGGCTCC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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