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MYH7 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MYH7
Entrez Gene ID 4625
Full Name myosin heavy chain 7
Synonyms CMD1S,CMH1,MPD1,MYHCB,SPMD,SPMM
General protein information
Preferred Names

myosin heavy chain 7

Names

myosin-7
cardiac muscle myosin heavy chain 7 beta
myHC-beta
myhc-slow
myopathy, distal 1
myosin 7
myosin heavy chain beta-subunit
myosin heavy chain slow isoform
myosin heavy chain, cardiac muscle beta isoform
myosin, heavy chain 7, cardiac muscle, beta
myosin, heavy polypeptide 7, cardiac muscle, beta
rhabdomyosarcoma antigen MU-RMS-40.7A

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

14

14q11.2

Summary Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy. [provided by RefSeq, Jul 2008].
Disorder MIM:

160760

Disorder Html: Cardiomyopathy, familial hypertrophic, 1, 192600 (3); Cardiomyopathy,

mRNA and Protein(s)

mRNA Protein Name
NM_000257.3 NP_000248.2 myosin-7
XM_017021340.1 XP_016876829.1 myosin-7 isoform X1



Danio rerio (zebrafish) smyhc1 NP_001018343.1
Danio rerio (zebrafish) vmhcl NP_001070932.2
Drosophila melanogaster (fruit fly) Mhc NP_001246049.1
Rattus norvegicus (Norway rat) Myh7 NP_058936.1
Danio rerio (zebrafish) LOC100329748 XP_002666778.1
Eremothecium gossypii AGOS_ACR068W NP_983470.2
Homo sapiens (human) MYH7 NP_000248.2
Canis lupus familiaris (dog) MYH7 NP_001107183.1
Mus musculus (house mouse) Myh7 NP_542766.1
Danio rerio (zebrafish) vmhc NP_001106204.1
Danio rerio (zebrafish) si:ch211-24n20.3 XP_001923213.1
Danio rerio (zebrafish) smyhc2 NP_001096096.2
Danio rerio (zebrafish) LOC100329813 XP_002666779.1
Saccharomyces cerevisiae (baker's yeast) MYO1 NP_011888.1
Kluyveromyces lactis KLLA0E11595g XP_454473.1
Pan troglodytes (chimpanzee) MYH7 XP_001150786.3
Bos taurus (cattle) MYH7 NP_777152.1
Danio rerio (zebrafish) LOC100329670 XP_002666777.2
Danio rerio (zebrafish) LOC567740 XP_696132.3
Anopheles gambiae (African malaria mosquito) AgaP_AGAP010147 XP_319309.3
Xenopus tropicalis (tropical clawed frog) myh7 XP_002939061.1
Gallus gallus (chicken) MYH7 NP_001001302.1
Danio rerio (zebrafish) smyhc3 NP_001129995.1
Schizosaccharomyces pombe (fission yeast) myo2 NP_588114.1


Related articles in PubMed

Genetic anticipation in a special form of hypertrophic cardiomyopathy with sudden cardiac death in a family with 74 members across 5 generations.
Guo X, Fan C, Wang Y, Wang M, Cai C, Yang Y, Zhao S, Duan F, Li Y
Medicine96(11)e6249(2017 Mar)

Spectrum of Mutations in Hypertrophic Cardiomyopathy Genes Among Tunisian Patients.
Jaafar N, Gómez J, Kammoun I, Zairi I, Amara WB, Kachboura S, Kraiem S, Hammami M, Iglesias S, Alonso B, Coto E
Genetic testing and molecular biomarkers20(11)674-679(2016 Nov)

Two novel MYH7 proline substitutions cause Laing Distal Myopathy-like phenotypes with variable expressivity and neck extensor contracture.
Feinstein-Linial M, Buvoli M, Buvoli A, Sadeh M, Dabby R, Straussberg R, Shelef I, Dayan D, Leinwand LA, Birk OS
BMC medical genetics17(1)57(2016 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors demonstrate myosin tail (S2)-dependent functional regulation of actin-activated human beta-cardiac myosin ATPase. In addition, they show that both S2 and MyBP-C bind to S1 and that phosphorylation of either S1 or MyBP-C weakens these interactions.
Title: The myosin mesa and the basis of hypercontractility caused by hypertrophic cardiomyopathy mutations.

family members who carried both the MYH7-A719H and MYOZ2-L169G mutations had more severe symptoms of hypertrophic cardiomyopathy, including sudden cardiac death, than those with only the MYH7 mutation
Title: Genetic anticipation in a special form of hypertrophic cardiomyopathy with sudden cardiac death in a family with 74 members across 5 generations.

Study shows lack of phenotypic differences between MYH7- and MYBPC3-associated hypertrophic cardiomyopathy when assessed by cardiac magnetic resonance imaging.
Title: Lack of Phenotypic Differences by Cardiovascular Magnetic Resonance Imaging in MYH7 (β-Myosin Heavy Chain)- Versus MYBPC3 (Myosin-Binding Protein C)-Related Hypertrophic Cardiomyopathy.

ur results suggest that the basis for the pathogenic effect of the R1845W and H1901L mutations of the MYH7 are primarily structural rather than functional. Further analyses are needed to identify the primary trigger for the histological changes seen in muscle biopsies of patients with L1793P and E1883K mutations.
Title: Myosin Storage Myopathy in C. elegans and Human Cultured Muscle Cells.

Our study supports that mutations in MYH7 and MYBPC3 should be the first focus of moleculargenetic analysis in HCM, and that mutations in TNNT2 have a low prevalence in Brazilian population. All mutations detected were missense mutations, whereas two mutations in MYH7 had not been described before.
Title: Prevalence and Phenotypic Expression of Mutations in the MYH7, MYBPC3 and TNNT2 Genes in Families with Hypertrophic Cardiomyopathy in the South of Brazil: A Cross-Sectional Study.

The following MYH7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu28301 NM_000257.3
Latest version!
Homo sapiens myosin heavy chain 7 (MYH7), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 22 $923.30
$1319.00
OHu28301 XM_017021340.1
Latest version!
Homo sapiens myosin, heavy chain 7, cardiac muscle, beta (MYH7), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 22 $923.30
$1319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu28301
Clone ID Related Accession (Same CDS sequence) NM_000257.3 , XM_017021340.1
Accession Version NM_000257.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5808bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product myosin-7
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF834726.1, EU747717.1, M58018.1 and BC112173.1. On or before Jun 20, 2014 this sequence version replaced XM_005267696.1, NM_000257.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU747717.1, M58018.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
ATGGGAGATT CGGAGATGGC AGTCTTTGGG GCTGCCGCCC CCTACCTGCG CAAGTCAGAG 
AAGGAGCGGC TAGAAGCGCA GACCAGGCCT TTTGACCTCA AGAAGGATGT CTTCGTGCCT
GATGACAAAC AGGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACT
GCCGAGACCG AGTATGGCAA GACAGTGACC GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC
CCACCCAAGT TCGACAAAAT CGAGGACATG GCCATGCTGA CCTTCCTGCA TGAGCCCGCG
GTGCTCTACA ACCTCAAGGA TCGCTACGGC TCCTGGATGA TCTACACCTA CTCGGGCCTC
TTCTGTGTCA CCGTCAACCC TTACAAGTGG CTGCCGGTGT ACACTCCTGA GGTGGTGGCT
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCGCCCCACA TCTTCTCCAT CTCCGACAAC
GCCTATCAGT ACATGCTGAC AGACAGAGAA AACCAGTCCA TCCTGATCAC CGGAGAATCC
GGAGCAGGGA AGACAGTCAA CACCAAGAGG GTCATCCAGT ACTTTGCTGT TATTGCAGCC
ATTGGGGACC GCAGCAAGAA GGACCAGAGC CCGGGCAAGG GCACCCTGGA GGACCAGATC
ATCCAGGCCA ACCCTGCTCT GGAGGCCTTT GGCAATGCCA AGACCGTCCG GAACGACAAC
TCCTCCCGCT TCGGGAAATT CATTCGAATT CATTTTGGGG CAACAGGAAA GTTGGCATCT
GCAGACATAG AGACCTATCT TCTGGAAAAA TCCAGAGTTA TTTTCCAGCT GAAAGCAGAG
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAACAAAA AGCCTGAGCT GCTGGACATG
CTGCTGATCA CCAACAACCC CTACGATTAT GCATTCATCT CCCAAGGAGA GACCACCGTG
GCCTCCATTG ATGACGCTGA GGAGCTCATG GCCACTGATA ACGCTTTTGA TGTGCTGGGC
TTCACTTCAG AGGAGAAAAA CTCCATGTAT AAGCTGACAG GCGCCATCAT GCACTTTGGA
AACATGAAGT TCAAGCTGAA GCAGCGGGAG GAGCAGGCGG AGCCAGACGG CACTGAAGAG
GCTGACAAGT CTGCCTACCT CATGGGGCTG AACTCAGCCG ACCTGCTCAA GGGGCTGTGC
CACCCTCGGG TGAAAGTGGG CAATGAGTAC GTCACCAAGG GGCAGAATGT CCAGCAGGTG
ATATATGCCA CTGGGGCACT GGCCAAGGCA GTGTATGAGA GGATGTTCAA CTGGATGGTG
ACGCGCATCA ATGCCACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGAGTCCTG
GACATCGCTG GCTTCGAGAT CTTCGATTTC AACAGCTTTG AGCAGCTCTG CATCAACTTC
ACCAACGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TTGTGCTGGA GCAGGAGGAG
TACAAGAAGG AGGGCATCGA GTGGACATTC ATTGACTTTG GCATGGACCT GCAGGCCTGC
ATTGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAAGAGGA GTGCATGTTC
CCCAAGGCCA CCGACATGAC CTTCAAGGCC AAGCTGTTTG ACAACCACCT GGGCAAATCC
GCCAACTTCC AGAAGCCACG CAATATCAAG GGGAAGCCTG AAGCCCACTT CTCCCTGATC
CACTATGCCG GCATCGTGGA CTACAACATC ATTGGCTGGC TGCAGAAGAA CAAGGATCCT
CTCAATGAGA CTGTCGTGGG CTTGTATCAG AAGTCTTCCC TCAAGCTGCT CAGCACCCTG
TTTGCCAACT ATGCTGGGGC TGATGCGCCT ATTGAGAAGG GCAAAGGCAA GGCCAAGAAA
GGCTCGTCCT TTCAGACTGT GTCAGCTCTG CACAGGGAAA ATCTGAACAA GCTGATGACC
AACTTGCGCT CCACCCATCC CCACTTTGTA CGTTGTATCA TCCCTAATGA GACAAAGTCT
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GCTGCAATGG TGTGCTGGAG
GGCATCCGCA TCTGCAGGAA AGGCTTCCCC AACCGCATCC TCTACGGGGA CTTCCGGCAG
AGGTATCGCA TCCTGAACCC AGCGGCCATC CCTGAGGGAC AGTTCATTGA TAGCAGGAAG
GGGGCAGAGA AGCTGCTCAG CTCCCTGGAC ATTGATCACA ACCAGTACAA GTTTGGCCAC
ACCAAGGTGT TCTTCAAGGC CGGGCTGCTG GGGCTGCTGG AGGAAATGAG GGACGAGAGG
CTGAGCCGCA TCATCACGCG TATCCAGGCC CAGTCCCGAG GTGTGCTCGC CAGAATGGAG
TACAAAAAGC TGCTGGAACG TAGAGACTCC CTGCTGGTAA TCCAGTGGAA CATTCGGGCC
TTCATGGGGG TCAAGAATTG GCCCTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG
AAGAGTGCAG AAAGAGAGAA GGAGATGGCC TCCATGAAGG AGGAGTTCAC ACGCCTCAAA
GAGGCGCTAG AGAAGTCCGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT
GCTGAGGAGC GCTGTGATCA GCTGATCAAA AACAAGATTC AGCTGGAGGC CAAGGTGAAG
GAGATGAACG AGAGGCTGGA GGATGAGGAG GAGATGAATG CTGAGCTCAC TGCCAAGAAG
CGCAAGCTGG AAGATGAGTG CTCAGAGCTC AAAAGGGACA TCGATGATCT GGAGCTGACA
CTGGCCAAAG TGGAGAAGGA GAAACACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG
GAGATGGCTG GGCTGGATGA GATCATTGCC AAGCTGACCA AGGAGAAGAA AGCTCTGCAA
GAGGCCCACC AACAGGCTCT GGATGACCTT CAGGCCGAGG AGGACAAGGT CAACACCCTG
ACTAAGGCCA AAGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAAGGATC CCTGGAGCAA
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCGAAGCGGA AGCTGGAGGG CGACCTGAAG
CTGACCCAGG AGAGCATCAT GGACCTGGAG AATGACAAGC AGCAGCTGGA TGAGCGGCTG
AAAAAAAAAG ACTTTGAGCT GAATGCTCTC AACGCAAGGA TTGAGGATGA ACAGGCCCTC
GGCAGCCAGC TGCAGAAGAA GCTCAAGGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG
GAGCTGGAGG CCGAGCGCAC CGCCAGGGCT AAGGTGGAGA AGCTGCGCTC AGACCTGTCT
CGGGAGCTGG AGGAGATCAG CGAGCGGCTG GAAGAGGCCG GCGGGGCCAC GTCCGTGCAG
ATCGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTGGAGGAG
GCCACGCTGC AGCACGAGGC CACTGCCGCG GCCCTGCGCA AGAAGCACGC CGACAGCGTG
GCCGAGCTGG GCGAGCAGAT CGACAACCTG CAGCGGGTGA AGCAGAAGCT GGAGAAGGAG
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTCACCTCCA ACATGGAGCA GATCATCAAG
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCTTGGAAG ACCAGATGAA TGAGCACCGG
AGCAAGGCGG AGGAGACCCA GCGTTCTGTC AACGACCTCA CCAGCCAGCG GGCCAAGTTG
CAAACCGAGA ATGGTGAGCT GTCCCGGCAG CTGGATGAGA AGGAGGCACT GATCTCCCAG
CTGACCCGAG GCAAGCTCAC CTACACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG
GAGGAGGTTA AGGCGAAGAA CGCCCTGGCC CACGCACTGC AGTCGGCCCG GCATGACTGC
GACCTGCTGC GGGAGCAGTA CGAGGAGGAG ACGGAGGCCA AGGCCGAGCT GCAGCGCGTC
CTTTCCAAGG CCAACTCGGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATT
CAGCGGACTG AGGAGCTCGA GGAGGCCAAG AAGAAGCTGG CCCAGCGGCT GCAGGAAGCT
GAGGAGGCCG TGGAGGCTGT TAATGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACCGG
CTACAGAATG AGATCGAGGA CTTGATGGTG GACGTAGAGC GCTCCAATGC TGCTGCTGCA
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CCGAGTGGAA GCAGAAGTAT
GAGGAGTCGC AGTCGGAGCT GGAGTCCTCG CAGAAGGAGG CTCGCTCCCT CAGCACAGAG
CTCTTCAAAC TCAAGAACGC CTATGAGGAG TCCCTGGAAC ATCTGGAGAC CTTCAAGCGG
GAGAACAAAA ACCTGCAGGA GGAGATCTCC GACTTGACTG AGCAGTTGGG TTCCAGCGGA
AAGACTATCC ATGAGCTGGA GAAGGTCCGA AAGCAGCTGG AGGCCGAGAA GATGGAGCTG
CAGTCAGCCC TGGAGGAGGC CGAGGCCTCC CTGGAGCACG AGGAGGGCAA GATCCTCCGG
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAGC GGAAGCTGGC AGAGAAGGAC
GAGGAGATGG AACAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCGCT GCAGACCTCC
CTGGACGCAG AGACACGCAG CCGCAACGAG GCCCTGAGGG TGAAGAAGAA GATGGAAGGA
GACCTCAATG AGATGGAGAT CCAGCTCAGC CACGCCAACC GCATGGCCGC CGAGGCCCAG
AAGCAAGTCA AGAGCCTCCA GAGCTTGTTG AAGGACACCC AGATTCAGCT GGACGATGCA
GTCCGTGCCA ACGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG
CTGCAGGCTG AGCTGGAGGA GTTGCGTGCC GTGGTGGAGC AGACAGAGCG GTCCCGGAAG
CTGGCGGAGC AGGAGCTGAT TGAGACTAGT GAGCGGGTGC AGCTGCTGCA TTCCCAGAAC
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GATGCTGACC TGTCCCAGCT CCAGACTGAA
GTGGAGGAGG CAGTGCAGGA GTGCAGGAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACG
GATGCCGCCA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG
CGCATGAAGA AGAACATGGA ACAGACCATT AAGGACCTGC AGCACCGGCT GGACGAAGCC
GAGCAGATCG CCCTCAAGGG CGGCAAGAAG CAGCTGCAGA AGCTGGAAGC GCGGGTGCGG
GAGCTGGAGA ATGAGCTGGA GGCCGAGCAG AAGCGCAACG CAGAGTCGGT GAAGGGCATG
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CGGAGGAGGA CAGGAAAAAC
CTGCTGCGGC TGCAGGACCT GGTAGACAAG CTGCAGCTAA AGGTCAAGGC CTACAAGCGC
CAGGCCGAGG AGGCGGAGGA GCAAGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG
CACGAGCTGG ATGAGGCAGA GGAGCGGGCG GACATCGCCG AGTCCCAGGT CAACAAGCTG
CGGGCCAAGA GCCGTGACAT TGGCACGAAG GGCTTGAATG AGGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000248.2
CDS132..5939
Misc Feature(1)102..104
Misc Feature(2)234..347
Misc Feature(3)390..2429
Misc Feature(4)426..2429
Misc Feature(5)order(507..533,663..686,828..860,1512..1529)
Misc Feature(6)507..533
Misc Feature(7)516..518
Misc Feature(8)663..686
Misc Feature(9)828..860
Misc Feature(10)1512..1529
Misc Feature(11)order(1599..1634,1641..1670)
Misc Feature(12)2094..2162
Misc Feature(13)2220..2249
Misc Feature(14)2256..2429
Misc Feature(15)2400..2444
Misc Feature(16)2667..5900
Misc Feature(17)2766..3254
Misc Feature(18)<3525..4010
Misc Feature(19)3825..4403
Misc Feature(20)4659..4661
Misc Feature(21)5349..>5621
Exon (1)1..67
Exon (2)68..123
Exon (3)124..332
Exon (4)333..476
Exon (5)477..633
Exon (6)634..661
Exon (7)662..770
Exon (8)771..863
Exon (9)864..927
Exon (10)928..1026
Exon (11)1027..1130
Exon (12)1131..1269
Exon (13)1270..1388
Exon (14)1389..1538
Exon (15)1539..1709
Exon (16)1710..2019
Exon (17)2020..2087
Exon (18)2088..2175
Exon (19)2176..2293
Exon (20)2294..2417
Exon (21)2418..2554
Exon (22)2555..2810
Exon (23)2811..3053
Exon (24)3054..3230
Exon (25)3231..3376
Exon (26)3377..3467
Exon (27)3468..3857
Exon (28)3858..3984
Exon (29)3985..4103
Exon (30)4104..4300
Exon (31)4301..4484
Exon (32)4485..4650
Exon (33)4651..4775
Exon (34)4776..5084
Exon (35)5085..5288
Exon (36)5289..5414
Exon (37)5415..5690
Exon (38)5691..5786
Exon (39)5787..5921
Exon (40)5922..6055
Translation

Target ORF information:

RefSeq Version NM_000257.3
Organism Homo sapiens(Human)
Definition Homo sapiens myosin heavy chain 7 (MYH7), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000257.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
ATGGGAGATT CGGAGATGGC AGTCTTTGGG GCTGCCGCCC CCTACCTGCG CAAGTCAGAG 
AAGGAGCGGC TAGAAGCGCA GACCAGGCCT TTTGACCTCA AGAAGGATGT CTTCGTGCCT
GATGACAAAC AGGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACT
GCCGAGACCG AGTATGGCAA GACAGTGACC GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC
CCACCCAAGT TCGACAAAAT CGAGGACATG GCCATGCTGA CCTTCCTGCA TGAGCCCGCG
GTGCTCTACA ACCTCAAGGA TCGCTACGGC TCCTGGATGA TCTACACCTA CTCGGGCCTC
TTCTGTGTCA CCGTCAACCC TTACAAGTGG CTGCCGGTGT ACACTCCTGA GGTGGTGGCT
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCGCCCCACA TCTTCTCCAT CTCCGACAAC
GCCTATCAGT ACATGCTGAC AGACAGAGAA AACCAGTCCA TCCTGATCAC CGGAGAATCC
GGAGCAGGGA AGACAGTCAA CACCAAGAGG GTCATCCAGT ACTTTGCTGT TATTGCAGCC
ATTGGGGACC GCAGCAAGAA GGACCAGAGC CCGGGCAAGG GCACCCTGGA GGACCAGATC
ATCCAGGCCA ACCCTGCTCT GGAGGCCTTT GGCAATGCCA AGACCGTCCG GAACGACAAC
TCCTCCCGCT TCGGGAAATT CATTCGAATT CATTTTGGGG CAACAGGAAA GTTGGCATCT
GCAGACATAG AGACCTATCT TCTGGAAAAA TCCAGAGTTA TTTTCCAGCT GAAAGCAGAG
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAACAAAA AGCCTGAGCT GCTGGACATG
CTGCTGATCA CCAACAACCC CTACGATTAT GCATTCATCT CCCAAGGAGA GACCACCGTG
GCCTCCATTG ATGACGCTGA GGAGCTCATG GCCACTGATA ACGCTTTTGA TGTGCTGGGC
TTCACTTCAG AGGAGAAAAA CTCCATGTAT AAGCTGACAG GCGCCATCAT GCACTTTGGA
AACATGAAGT TCAAGCTGAA GCAGCGGGAG GAGCAGGCGG AGCCAGACGG CACTGAAGAG
GCTGACAAGT CTGCCTACCT CATGGGGCTG AACTCAGCCG ACCTGCTCAA GGGGCTGTGC
CACCCTCGGG TGAAAGTGGG CAATGAGTAC GTCACCAAGG GGCAGAATGT CCAGCAGGTG
ATATATGCCA CTGGGGCACT GGCCAAGGCA GTGTATGAGA GGATGTTCAA CTGGATGGTG
ACGCGCATCA ATGCCACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGAGTCCTG
GACATCGCTG GCTTCGAGAT CTTCGATTTC AACAGCTTTG AGCAGCTCTG CATCAACTTC
ACCAACGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TTGTGCTGGA GCAGGAGGAG
TACAAGAAGG AGGGCATCGA GTGGACATTC ATTGACTTTG GCATGGACCT GCAGGCCTGC
ATTGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAAGAGGA GTGCATGTTC
CCCAAGGCCA CCGACATGAC CTTCAAGGCC AAGCTGTTTG ACAACCACCT GGGCAAATCC
GCCAACTTCC AGAAGCCACG CAATATCAAG GGGAAGCCTG AAGCCCACTT CTCCCTGATC
CACTATGCCG GCATCGTGGA CTACAACATC ATTGGCTGGC TGCAGAAGAA CAAGGATCCT
CTCAATGAGA CTGTCGTGGG CTTGTATCAG AAGTCTTCCC TCAAGCTGCT CAGCACCCTG
TTTGCCAACT ATGCTGGGGC TGATGCGCCT ATTGAGAAGG GCAAAGGCAA GGCCAAGAAA
GGCTCGTCCT TTCAGACTGT GTCAGCTCTG CACAGGGAAA ATCTGAACAA GCTGATGACC
AACTTGCGCT CCACCCATCC CCACTTTGTA CGTTGTATCA TCCCTAATGA GACAAAGTCT
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GCTGCAATGG TGTGCTGGAG
GGCATCCGCA TCTGCAGGAA AGGCTTCCCC AACCGCATCC TCTACGGGGA CTTCCGGCAG
AGGTATCGCA TCCTGAACCC AGCGGCCATC CCTGAGGGAC AGTTCATTGA TAGCAGGAAG
GGGGCAGAGA AGCTGCTCAG CTCCCTGGAC ATTGATCACA ACCAGTACAA GTTTGGCCAC
ACCAAGGTGT TCTTCAAGGC CGGGCTGCTG GGGCTGCTGG AGGAAATGAG GGACGAGAGG
CTGAGCCGCA TCATCACGCG TATCCAGGCC CAGTCCCGAG GTGTGCTCGC CAGAATGGAG
TACAAAAAGC TGCTGGAACG TAGAGACTCC CTGCTGGTAA TCCAGTGGAA CATTCGGGCC
TTCATGGGGG TCAAGAATTG GCCCTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG
AAGAGTGCAG AAAGAGAGAA GGAGATGGCC TCCATGAAGG AGGAGTTCAC ACGCCTCAAA
GAGGCGCTAG AGAAGTCCGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT
GCTGAGGAGC GCTGTGATCA GCTGATCAAA AACAAGATTC AGCTGGAGGC CAAGGTGAAG
GAGATGAACG AGAGGCTGGA GGATGAGGAG GAGATGAATG CTGAGCTCAC TGCCAAGAAG
CGCAAGCTGG AAGATGAGTG CTCAGAGCTC AAAAGGGACA TCGATGATCT GGAGCTGACA
CTGGCCAAAG TGGAGAAGGA GAAACACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG
GAGATGGCTG GGCTGGATGA GATCATTGCC AAGCTGACCA AGGAGAAGAA AGCTCTGCAA
GAGGCCCACC AACAGGCTCT GGATGACCTT CAGGCCGAGG AGGACAAGGT CAACACCCTG
ACTAAGGCCA AAGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAAGGATC CCTGGAGCAA
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCGAAGCGGA AGCTGGAGGG CGACCTGAAG
CTGACCCAGG AGAGCATCAT GGACCTGGAG AATGACAAGC AGCAGCTGGA TGAGCGGCTG
AAAAAAAAAG ACTTTGAGCT GAATGCTCTC AACGCAAGGA TTGAGGATGA ACAGGCCCTC
GGCAGCCAGC TGCAGAAGAA GCTCAAGGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG
GAGCTGGAGG CCGAGCGCAC CGCCAGGGCT AAGGTGGAGA AGCTGCGCTC AGACCTGTCT
CGGGAGCTGG AGGAGATCAG CGAGCGGCTG GAAGAGGCCG GCGGGGCCAC GTCCGTGCAG
ATCGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTGGAGGAG
GCCACGCTGC AGCACGAGGC CACTGCCGCG GCCCTGCGCA AGAAGCACGC CGACAGCGTG
GCCGAGCTGG GCGAGCAGAT CGACAACCTG CAGCGGGTGA AGCAGAAGCT GGAGAAGGAG
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTCACCTCCA ACATGGAGCA GATCATCAAG
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCTTGGAAG ACCAGATGAA TGAGCACCGG
AGCAAGGCGG AGGAGACCCA GCGTTCTGTC AACGACCTCA CCAGCCAGCG GGCCAAGTTG
CAAACCGAGA ATGGTGAGCT GTCCCGGCAG CTGGATGAGA AGGAGGCACT GATCTCCCAG
CTGACCCGAG GCAAGCTCAC CTACACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG
GAGGAGGTTA AGGCGAAGAA CGCCCTGGCC CACGCACTGC AGTCGGCCCG GCATGACTGC
GACCTGCTGC GGGAGCAGTA CGAGGAGGAG ACGGAGGCCA AGGCCGAGCT GCAGCGCGTC
CTTTCCAAGG CCAACTCGGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATT
CAGCGGACTG AGGAGCTCGA GGAGGCCAAG AAGAAGCTGG CCCAGCGGCT GCAGGAAGCT
GAGGAGGCCG TGGAGGCTGT TAATGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACCGG
CTACAGAATG AGATCGAGGA CTTGATGGTG GACGTAGAGC GCTCCAATGC TGCTGCTGCA
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CCGAGTGGAA GCAGAAGTAT
GAGGAGTCGC AGTCGGAGCT GGAGTCCTCG CAGAAGGAGG CTCGCTCCCT CAGCACAGAG
CTCTTCAAAC TCAAGAACGC CTATGAGGAG TCCCTGGAAC ATCTGGAGAC CTTCAAGCGG
GAGAACAAAA ACCTGCAGGA GGAGATCTCC GACTTGACTG AGCAGTTGGG TTCCAGCGGA
AAGACTATCC ATGAGCTGGA GAAGGTCCGA AAGCAGCTGG AGGCCGAGAA GATGGAGCTG
CAGTCAGCCC TGGAGGAGGC CGAGGCCTCC CTGGAGCACG AGGAGGGCAA GATCCTCCGG
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAGC GGAAGCTGGC AGAGAAGGAC
GAGGAGATGG AACAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCGCT GCAGACCTCC
CTGGACGCAG AGACACGCAG CCGCAACGAG GCCCTGAGGG TGAAGAAGAA GATGGAAGGA
GACCTCAATG AGATGGAGAT CCAGCTCAGC CACGCCAACC GCATGGCCGC CGAGGCCCAG
AAGCAAGTCA AGAGCCTCCA GAGCTTGTTG AAGGACACCC AGATTCAGCT GGACGATGCA
GTCCGTGCCA ACGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG
CTGCAGGCTG AGCTGGAGGA GTTGCGTGCC GTGGTGGAGC AGACAGAGCG GTCCCGGAAG
CTGGCGGAGC AGGAGCTGAT TGAGACTAGT GAGCGGGTGC AGCTGCTGCA TTCCCAGAAC
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GATGCTGACC TGTCCCAGCT CCAGACTGAA
GTGGAGGAGG CAGTGCAGGA GTGCAGGAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACG
GATGCCGCCA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG
CGCATGAAGA AGAACATGGA ACAGACCATT AAGGACCTGC AGCACCGGCT GGACGAAGCC
GAGCAGATCG CCCTCAAGGG CGGCAAGAAG CAGCTGCAGA AGCTGGAAGC GCGGGTGCGG
GAGCTGGAGA ATGAGCTGGA GGCCGAGCAG AAGCGCAACG CAGAGTCGGT GAAGGGCATG
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CGGAGGAGGA CAGGAAAAAC
CTGCTGCGGC TGCAGGACCT GGTAGACAAG CTGCAGCTAA AGGTCAAGGC CTACAAGCGC
CAGGCCGAGG AGGCGGAGGA GCAAGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG
CACGAGCTGG ATGAGGCAGA GGAGCGGGCG GACATCGCCG AGTCCCAGGT CAACAAGCTG
CGGGCCAAGA GCCGTGACAT TGGCACGAAG GGCTTGAATG AGGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu28301
Clone ID Related Accession (Same CDS sequence) NM_000257.3 , XM_017021340.1
Accession Version XM_017021340.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 5808bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product myosin-7 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
ATGGGAGATT CGGAGATGGC AGTCTTTGGG GCTGCCGCCC CCTACCTGCG CAAGTCAGAG 
AAGGAGCGGC TAGAAGCGCA GACCAGGCCT TTTGACCTCA AGAAGGATGT CTTCGTGCCT
GATGACAAAC AGGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACT
GCCGAGACCG AGTATGGCAA GACAGTGACC GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC
CCACCCAAGT TCGACAAAAT CGAGGACATG GCCATGCTGA CCTTCCTGCA TGAGCCCGCG
GTGCTCTACA ACCTCAAGGA TCGCTACGGC TCCTGGATGA TCTACACCTA CTCGGGCCTC
TTCTGTGTCA CCGTCAACCC TTACAAGTGG CTGCCGGTGT ACACTCCTGA GGTGGTGGCT
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCGCCCCACA TCTTCTCCAT CTCCGACAAC
GCCTATCAGT ACATGCTGAC AGACAGAGAA AACCAGTCCA TCCTGATCAC CGGAGAATCC
GGAGCAGGGA AGACAGTCAA CACCAAGAGG GTCATCCAGT ACTTTGCTGT TATTGCAGCC
ATTGGGGACC GCAGCAAGAA GGACCAGAGC CCGGGCAAGG GCACCCTGGA GGACCAGATC
ATCCAGGCCA ACCCTGCTCT GGAGGCCTTT GGCAATGCCA AGACCGTCCG GAACGACAAC
TCCTCCCGCT TCGGGAAATT CATTCGAATT CATTTTGGGG CAACAGGAAA GTTGGCATCT
GCAGACATAG AGACCTATCT TCTGGAAAAA TCCAGAGTTA TTTTCCAGCT GAAAGCAGAG
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAACAAAA AGCCTGAGCT GCTGGACATG
CTGCTGATCA CCAACAACCC CTACGATTAT GCATTCATCT CCCAAGGAGA GACCACCGTG
GCCTCCATTG ATGACGCTGA GGAGCTCATG GCCACTGATA ACGCTTTTGA TGTGCTGGGC
TTCACTTCAG AGGAGAAAAA CTCCATGTAT AAGCTGACAG GCGCCATCAT GCACTTTGGA
AACATGAAGT TCAAGCTGAA GCAGCGGGAG GAGCAGGCGG AGCCAGACGG CACTGAAGAG
GCTGACAAGT CTGCCTACCT CATGGGGCTG AACTCAGCCG ACCTGCTCAA GGGGCTGTGC
CACCCTCGGG TGAAAGTGGG CAATGAGTAC GTCACCAAGG GGCAGAATGT CCAGCAGGTG
ATATATGCCA CTGGGGCACT GGCCAAGGCA GTGTATGAGA GGATGTTCAA CTGGATGGTG
ACGCGCATCA ATGCCACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGAGTCCTG
GACATCGCTG GCTTCGAGAT CTTCGATTTC AACAGCTTTG AGCAGCTCTG CATCAACTTC
ACCAACGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TTGTGCTGGA GCAGGAGGAG
TACAAGAAGG AGGGCATCGA GTGGACATTC ATTGACTTTG GCATGGACCT GCAGGCCTGC
ATTGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAAGAGGA GTGCATGTTC
CCCAAGGCCA CCGACATGAC CTTCAAGGCC AAGCTGTTTG ACAACCACCT GGGCAAATCC
GCCAACTTCC AGAAGCCACG CAATATCAAG GGGAAGCCTG AAGCCCACTT CTCCCTGATC
CACTATGCCG GCATCGTGGA CTACAACATC ATTGGCTGGC TGCAGAAGAA CAAGGATCCT
CTCAATGAGA CTGTCGTGGG CTTGTATCAG AAGTCTTCCC TCAAGCTGCT CAGCACCCTG
TTTGCCAACT ATGCTGGGGC TGATGCGCCT ATTGAGAAGG GCAAAGGCAA GGCCAAGAAA
GGCTCGTCCT TTCAGACTGT GTCAGCTCTG CACAGGGAAA ATCTGAACAA GCTGATGACC
AACTTGCGCT CCACCCATCC CCACTTTGTA CGTTGTATCA TCCCTAATGA GACAAAGTCT
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GCTGCAATGG TGTGCTGGAG
GGCATCCGCA TCTGCAGGAA AGGCTTCCCC AACCGCATCC TCTACGGGGA CTTCCGGCAG
AGGTATCGCA TCCTGAACCC AGCGGCCATC CCTGAGGGAC AGTTCATTGA TAGCAGGAAG
GGGGCAGAGA AGCTGCTCAG CTCCCTGGAC ATTGATCACA ACCAGTACAA GTTTGGCCAC
ACCAAGGTGT TCTTCAAGGC CGGGCTGCTG GGGCTGCTGG AGGAAATGAG GGACGAGAGG
CTGAGCCGCA TCATCACGCG TATCCAGGCC CAGTCCCGAG GTGTGCTCGC CAGAATGGAG
TACAAAAAGC TGCTGGAACG TAGAGACTCC CTGCTGGTAA TCCAGTGGAA CATTCGGGCC
TTCATGGGGG TCAAGAATTG GCCCTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG
AAGAGTGCAG AAAGAGAGAA GGAGATGGCC TCCATGAAGG AGGAGTTCAC ACGCCTCAAA
GAGGCGCTAG AGAAGTCCGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT
GCTGAGGAGC GCTGTGATCA GCTGATCAAA AACAAGATTC AGCTGGAGGC CAAGGTGAAG
GAGATGAACG AGAGGCTGGA GGATGAGGAG GAGATGAATG CTGAGCTCAC TGCCAAGAAG
CGCAAGCTGG AAGATGAGTG CTCAGAGCTC AAAAGGGACA TCGATGATCT GGAGCTGACA
CTGGCCAAAG TGGAGAAGGA GAAACACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG
GAGATGGCTG GGCTGGATGA GATCATTGCC AAGCTGACCA AGGAGAAGAA AGCTCTGCAA
GAGGCCCACC AACAGGCTCT GGATGACCTT CAGGCCGAGG AGGACAAGGT CAACACCCTG
ACTAAGGCCA AAGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAAGGATC CCTGGAGCAA
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCGAAGCGGA AGCTGGAGGG CGACCTGAAG
CTGACCCAGG AGAGCATCAT GGACCTGGAG AATGACAAGC AGCAGCTGGA TGAGCGGCTG
AAAAAAAAAG ACTTTGAGCT GAATGCTCTC AACGCAAGGA TTGAGGATGA ACAGGCCCTC
GGCAGCCAGC TGCAGAAGAA GCTCAAGGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG
GAGCTGGAGG CCGAGCGCAC CGCCAGGGCT AAGGTGGAGA AGCTGCGCTC AGACCTGTCT
CGGGAGCTGG AGGAGATCAG CGAGCGGCTG GAAGAGGCCG GCGGGGCCAC GTCCGTGCAG
ATCGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTGGAGGAG
GCCACGCTGC AGCACGAGGC CACTGCCGCG GCCCTGCGCA AGAAGCACGC CGACAGCGTG
GCCGAGCTGG GCGAGCAGAT CGACAACCTG CAGCGGGTGA AGCAGAAGCT GGAGAAGGAG
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTCACCTCCA ACATGGAGCA GATCATCAAG
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCTTGGAAG ACCAGATGAA TGAGCACCGG
AGCAAGGCGG AGGAGACCCA GCGTTCTGTC AACGACCTCA CCAGCCAGCG GGCCAAGTTG
CAAACCGAGA ATGGTGAGCT GTCCCGGCAG CTGGATGAGA AGGAGGCACT GATCTCCCAG
CTGACCCGAG GCAAGCTCAC CTACACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG
GAGGAGGTTA AGGCGAAGAA CGCCCTGGCC CACGCACTGC AGTCGGCCCG GCATGACTGC
GACCTGCTGC GGGAGCAGTA CGAGGAGGAG ACGGAGGCCA AGGCCGAGCT GCAGCGCGTC
CTTTCCAAGG CCAACTCGGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATT
CAGCGGACTG AGGAGCTCGA GGAGGCCAAG AAGAAGCTGG CCCAGCGGCT GCAGGAAGCT
GAGGAGGCCG TGGAGGCTGT TAATGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACCGG
CTACAGAATG AGATCGAGGA CTTGATGGTG GACGTAGAGC GCTCCAATGC TGCTGCTGCA
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CCGAGTGGAA GCAGAAGTAT
GAGGAGTCGC AGTCGGAGCT GGAGTCCTCG CAGAAGGAGG CTCGCTCCCT CAGCACAGAG
CTCTTCAAAC TCAAGAACGC CTATGAGGAG TCCCTGGAAC ATCTGGAGAC CTTCAAGCGG
GAGAACAAAA ACCTGCAGGA GGAGATCTCC GACTTGACTG AGCAGTTGGG TTCCAGCGGA
AAGACTATCC ATGAGCTGGA GAAGGTCCGA AAGCAGCTGG AGGCCGAGAA GATGGAGCTG
CAGTCAGCCC TGGAGGAGGC CGAGGCCTCC CTGGAGCACG AGGAGGGCAA GATCCTCCGG
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAGC GGAAGCTGGC AGAGAAGGAC
GAGGAGATGG AACAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCGCT GCAGACCTCC
CTGGACGCAG AGACACGCAG CCGCAACGAG GCCCTGAGGG TGAAGAAGAA GATGGAAGGA
GACCTCAATG AGATGGAGAT CCAGCTCAGC CACGCCAACC GCATGGCCGC CGAGGCCCAG
AAGCAAGTCA AGAGCCTCCA GAGCTTGTTG AAGGACACCC AGATTCAGCT GGACGATGCA
GTCCGTGCCA ACGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG
CTGCAGGCTG AGCTGGAGGA GTTGCGTGCC GTGGTGGAGC AGACAGAGCG GTCCCGGAAG
CTGGCGGAGC AGGAGCTGAT TGAGACTAGT GAGCGGGTGC AGCTGCTGCA TTCCCAGAAC
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GATGCTGACC TGTCCCAGCT CCAGACTGAA
GTGGAGGAGG CAGTGCAGGA GTGCAGGAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACG
GATGCCGCCA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG
CGCATGAAGA AGAACATGGA ACAGACCATT AAGGACCTGC AGCACCGGCT GGACGAAGCC
GAGCAGATCG CCCTCAAGGG CGGCAAGAAG CAGCTGCAGA AGCTGGAAGC GCGGGTGCGG
GAGCTGGAGA ATGAGCTGGA GGCCGAGCAG AAGCGCAACG CAGAGTCGGT GAAGGGCATG
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CGGAGGAGGA CAGGAAAAAC
CTGCTGCGGC TGCAGGACCT GGTAGACAAG CTGCAGCTAA AGGTCAAGGC CTACAAGCGC
CAGGCCGAGG AGGCGGAGGA GCAAGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG
CACGAGCTGG ATGAGGCAGA GGAGCGGGCG GACATCGCCG AGTCCCAGGT CAACAAGCTG
CGGGCCAAGA GCCGTGACAT TGGCACGAAG GGCTTGAATG AGGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016876829.1
CDS67..5874
Misc Feature(1)169..282
Misc Feature(2)325..2364
Misc Feature(3)361..2364
Misc Feature(4)order(442..468,598..621,763..795,1447..1464)
Misc Feature(5)442..468
Misc Feature(6)598..621
Misc Feature(7)763..795
Misc Feature(8)1447..1464
Misc Feature(9)order(1534..1569,1576..1605)
Misc Feature(10)2155..2184
Misc Feature(11)2191..2364
Misc Feature(12)2602..5835
Misc Feature(13)2701..3189
Misc Feature(14)<3460..3945
Misc Feature(15)3760..4338
Misc Feature(16)5284..>5556
Translation

Target ORF information:

RefSeq Version XM_017021340.1
Organism Homo sapiens(Human)
Definition Homo sapiens myosin, heavy chain 7, cardiac muscle, beta (MYH7), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017021340.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
ATGGGAGATT CGGAGATGGC AGTCTTTGGG GCTGCCGCCC CCTACCTGCG CAAGTCAGAG 
AAGGAGCGGC TAGAAGCGCA GACCAGGCCT TTTGACCTCA AGAAGGATGT CTTCGTGCCT
GATGACAAAC AGGAGTTTGT CAAGGCCAAG ATCGTGTCTC GAGAGGGTGG CAAAGTCACT
GCCGAGACCG AGTATGGCAA GACAGTGACC GTGAAGGAGG ACCAGGTGAT GCAGCAGAAC
CCACCCAAGT TCGACAAAAT CGAGGACATG GCCATGCTGA CCTTCCTGCA TGAGCCCGCG
GTGCTCTACA ACCTCAAGGA TCGCTACGGC TCCTGGATGA TCTACACCTA CTCGGGCCTC
TTCTGTGTCA CCGTCAACCC TTACAAGTGG CTGCCGGTGT ACACTCCTGA GGTGGTGGCT
GCCTACCGGG GCAAGAAGAG GAGCGAGGCC CCGCCCCACA TCTTCTCCAT CTCCGACAAC
GCCTATCAGT ACATGCTGAC AGACAGAGAA AACCAGTCCA TCCTGATCAC CGGAGAATCC
GGAGCAGGGA AGACAGTCAA CACCAAGAGG GTCATCCAGT ACTTTGCTGT TATTGCAGCC
ATTGGGGACC GCAGCAAGAA GGACCAGAGC CCGGGCAAGG GCACCCTGGA GGACCAGATC
ATCCAGGCCA ACCCTGCTCT GGAGGCCTTT GGCAATGCCA AGACCGTCCG GAACGACAAC
TCCTCCCGCT TCGGGAAATT CATTCGAATT CATTTTGGGG CAACAGGAAA GTTGGCATCT
GCAGACATAG AGACCTATCT TCTGGAAAAA TCCAGAGTTA TTTTCCAGCT GAAAGCAGAG
AGAGATTATC ACATTTTCTA CCAAATCCTG TCTAACAAAA AGCCTGAGCT GCTGGACATG
CTGCTGATCA CCAACAACCC CTACGATTAT GCATTCATCT CCCAAGGAGA GACCACCGTG
GCCTCCATTG ATGACGCTGA GGAGCTCATG GCCACTGATA ACGCTTTTGA TGTGCTGGGC
TTCACTTCAG AGGAGAAAAA CTCCATGTAT AAGCTGACAG GCGCCATCAT GCACTTTGGA
AACATGAAGT TCAAGCTGAA GCAGCGGGAG GAGCAGGCGG AGCCAGACGG CACTGAAGAG
GCTGACAAGT CTGCCTACCT CATGGGGCTG AACTCAGCCG ACCTGCTCAA GGGGCTGTGC
CACCCTCGGG TGAAAGTGGG CAATGAGTAC GTCACCAAGG GGCAGAATGT CCAGCAGGTG
ATATATGCCA CTGGGGCACT GGCCAAGGCA GTGTATGAGA GGATGTTCAA CTGGATGGTG
ACGCGCATCA ATGCCACCCT GGAGACCAAG CAGCCACGCC AGTACTTCAT AGGAGTCCTG
GACATCGCTG GCTTCGAGAT CTTCGATTTC AACAGCTTTG AGCAGCTCTG CATCAACTTC
ACCAACGAGA AGCTGCAGCA GTTCTTCAAC CACCACATGT TTGTGCTGGA GCAGGAGGAG
TACAAGAAGG AGGGCATCGA GTGGACATTC ATTGACTTTG GCATGGACCT GCAGGCCTGC
ATTGACCTCA TCGAGAAGCC CATGGGCATC ATGTCCATCC TGGAAGAGGA GTGCATGTTC
CCCAAGGCCA CCGACATGAC CTTCAAGGCC AAGCTGTTTG ACAACCACCT GGGCAAATCC
GCCAACTTCC AGAAGCCACG CAATATCAAG GGGAAGCCTG AAGCCCACTT CTCCCTGATC
CACTATGCCG GCATCGTGGA CTACAACATC ATTGGCTGGC TGCAGAAGAA CAAGGATCCT
CTCAATGAGA CTGTCGTGGG CTTGTATCAG AAGTCTTCCC TCAAGCTGCT CAGCACCCTG
TTTGCCAACT ATGCTGGGGC TGATGCGCCT ATTGAGAAGG GCAAAGGCAA GGCCAAGAAA
GGCTCGTCCT TTCAGACTGT GTCAGCTCTG CACAGGGAAA ATCTGAACAA GCTGATGACC
AACTTGCGCT CCACCCATCC CCACTTTGTA CGTTGTATCA TCCCTAATGA GACAAAGTCT
CCAGGGGTGA TGGACAACCC CCTGGTCATG CACCAGCTGC GCTGCAATGG TGTGCTGGAG
GGCATCCGCA TCTGCAGGAA AGGCTTCCCC AACCGCATCC TCTACGGGGA CTTCCGGCAG
AGGTATCGCA TCCTGAACCC AGCGGCCATC CCTGAGGGAC AGTTCATTGA TAGCAGGAAG
GGGGCAGAGA AGCTGCTCAG CTCCCTGGAC ATTGATCACA ACCAGTACAA GTTTGGCCAC
ACCAAGGTGT TCTTCAAGGC CGGGCTGCTG GGGCTGCTGG AGGAAATGAG GGACGAGAGG
CTGAGCCGCA TCATCACGCG TATCCAGGCC CAGTCCCGAG GTGTGCTCGC CAGAATGGAG
TACAAAAAGC TGCTGGAACG TAGAGACTCC CTGCTGGTAA TCCAGTGGAA CATTCGGGCC
TTCATGGGGG TCAAGAATTG GCCCTGGATG AAGCTCTACT TCAAGATCAA GCCGCTGCTG
AAGAGTGCAG AAAGAGAGAA GGAGATGGCC TCCATGAAGG AGGAGTTCAC ACGCCTCAAA
GAGGCGCTAG AGAAGTCCGA GGCTCGCCGC AAGGAGCTGG AGGAGAAGAT GGTGTCCCTG
CTGCAGGAGA AGAATGACCT GCAGCTCCAA GTGCAGGCGG AACAAGACAA CCTGGCAGAT
GCTGAGGAGC GCTGTGATCA GCTGATCAAA AACAAGATTC AGCTGGAGGC CAAGGTGAAG
GAGATGAACG AGAGGCTGGA GGATGAGGAG GAGATGAATG CTGAGCTCAC TGCCAAGAAG
CGCAAGCTGG AAGATGAGTG CTCAGAGCTC AAAAGGGACA TCGATGATCT GGAGCTGACA
CTGGCCAAAG TGGAGAAGGA GAAACACGCA ACAGAGAACA AGGTGAAAAA CCTGACAGAG
GAGATGGCTG GGCTGGATGA GATCATTGCC AAGCTGACCA AGGAGAAGAA AGCTCTGCAA
GAGGCCCACC AACAGGCTCT GGATGACCTT CAGGCCGAGG AGGACAAGGT CAACACCCTG
ACTAAGGCCA AAGTCAAGCT GGAGCAGCAA GTGGATGATC TGGAAGGATC CCTGGAGCAA
GAGAAGAAGG TGCGCATGGA CCTGGAGCGA GCGAAGCGGA AGCTGGAGGG CGACCTGAAG
CTGACCCAGG AGAGCATCAT GGACCTGGAG AATGACAAGC AGCAGCTGGA TGAGCGGCTG
AAAAAAAAAG ACTTTGAGCT GAATGCTCTC AACGCAAGGA TTGAGGATGA ACAGGCCCTC
GGCAGCCAGC TGCAGAAGAA GCTCAAGGAG CTTCAGGCAC GCATCGAGGA GCTGGAGGAG
GAGCTGGAGG CCGAGCGCAC CGCCAGGGCT AAGGTGGAGA AGCTGCGCTC AGACCTGTCT
CGGGAGCTGG AGGAGATCAG CGAGCGGCTG GAAGAGGCCG GCGGGGCCAC GTCCGTGCAG
ATCGAGATGA ACAAGAAGCG CGAGGCCGAG TTCCAGAAGA TGCGGCGGGA CCTGGAGGAG
GCCACGCTGC AGCACGAGGC CACTGCCGCG GCCCTGCGCA AGAAGCACGC CGACAGCGTG
GCCGAGCTGG GCGAGCAGAT CGACAACCTG CAGCGGGTGA AGCAGAAGCT GGAGAAGGAG
AAGAGCGAGT TCAAGCTGGA GCTGGATGAC GTCACCTCCA ACATGGAGCA GATCATCAAG
GCCAAGGCTA ACCTGGAGAA GATGTGCCGG ACCTTGGAAG ACCAGATGAA TGAGCACCGG
AGCAAGGCGG AGGAGACCCA GCGTTCTGTC AACGACCTCA CCAGCCAGCG GGCCAAGTTG
CAAACCGAGA ATGGTGAGCT GTCCCGGCAG CTGGATGAGA AGGAGGCACT GATCTCCCAG
CTGACCCGAG GCAAGCTCAC CTACACCCAG CAGCTGGAGG ACCTCAAGAG GCAGCTGGAG
GAGGAGGTTA AGGCGAAGAA CGCCCTGGCC CACGCACTGC AGTCGGCCCG GCATGACTGC
GACCTGCTGC GGGAGCAGTA CGAGGAGGAG ACGGAGGCCA AGGCCGAGCT GCAGCGCGTC
CTTTCCAAGG CCAACTCGGA GGTGGCCCAG TGGAGGACCA AGTATGAGAC GGACGCCATT
CAGCGGACTG AGGAGCTCGA GGAGGCCAAG AAGAAGCTGG CCCAGCGGCT GCAGGAAGCT
GAGGAGGCCG TGGAGGCTGT TAATGCCAAG TGCTCCTCGC TGGAGAAGAC CAAGCACCGG
CTACAGAATG AGATCGAGGA CTTGATGGTG GACGTAGAGC GCTCCAATGC TGCTGCTGCA
GCCCTGGACA AGAAGCAGAG GAACTTCGAC AAGATCCTGG CCGAGTGGAA GCAGAAGTAT
GAGGAGTCGC AGTCGGAGCT GGAGTCCTCG CAGAAGGAGG CTCGCTCCCT CAGCACAGAG
CTCTTCAAAC TCAAGAACGC CTATGAGGAG TCCCTGGAAC ATCTGGAGAC CTTCAAGCGG
GAGAACAAAA ACCTGCAGGA GGAGATCTCC GACTTGACTG AGCAGTTGGG TTCCAGCGGA
AAGACTATCC ATGAGCTGGA GAAGGTCCGA AAGCAGCTGG AGGCCGAGAA GATGGAGCTG
CAGTCAGCCC TGGAGGAGGC CGAGGCCTCC CTGGAGCACG AGGAGGGCAA GATCCTCCGG
GCCCAGCTGG AGTTCAACCA GATCAAGGCA GAGATCGAGC GGAAGCTGGC AGAGAAGGAC
GAGGAGATGG AACAGGCCAA GCGCAACCAC CTGCGGGTGG TGGACTCGCT GCAGACCTCC
CTGGACGCAG AGACACGCAG CCGCAACGAG GCCCTGAGGG TGAAGAAGAA GATGGAAGGA
GACCTCAATG AGATGGAGAT CCAGCTCAGC CACGCCAACC GCATGGCCGC CGAGGCCCAG
AAGCAAGTCA AGAGCCTCCA GAGCTTGTTG AAGGACACCC AGATTCAGCT GGACGATGCA
GTCCGTGCCA ACGACGACCT GAAGGAGAAC ATCGCCATCG TGGAGCGGCG CAACAACCTG
CTGCAGGCTG AGCTGGAGGA GTTGCGTGCC GTGGTGGAGC AGACAGAGCG GTCCCGGAAG
CTGGCGGAGC AGGAGCTGAT TGAGACTAGT GAGCGGGTGC AGCTGCTGCA TTCCCAGAAC
ACCAGCCTCA TCAACCAGAA GAAGAAGATG GATGCTGACC TGTCCCAGCT CCAGACTGAA
GTGGAGGAGG CAGTGCAGGA GTGCAGGAAT GCTGAGGAGA AGGCCAAGAA GGCCATCACG
GATGCCGCCA TGATGGCAGA GGAGCTGAAG AAGGAGCAGG ACACCAGCGC CCACCTGGAG
CGCATGAAGA AGAACATGGA ACAGACCATT AAGGACCTGC AGCACCGGCT GGACGAAGCC
GAGCAGATCG CCCTCAAGGG CGGCAAGAAG CAGCTGCAGA AGCTGGAAGC GCGGGTGCGG
GAGCTGGAGA ATGAGCTGGA GGCCGAGCAG AAGCGCAACG CAGAGTCGGT GAAGGGCATG
AGGAAGAGCG AGCGGCGCAT CAAGGAGCTC ACCTACCAGA CGGAGGAGGA CAGGAAAAAC
CTGCTGCGGC TGCAGGACCT GGTAGACAAG CTGCAGCTAA AGGTCAAGGC CTACAAGCGC
CAGGCCGAGG AGGCGGAGGA GCAAGCCAAC ACCAACCTGT CCAAGTTCCG CAAGGTGCAG
CACGAGCTGG ATGAGGCAGA GGAGCGGGCG GACATCGCCG AGTCCCAGGT CAACAAGCTG
CGGGCCAAGA GCCGTGACAT TGGCACGAAG GGCTTGAATG AGGAGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Genetic anticipation in a special form of hypertrophic cardiomyopathy with sudden cardiac death in a family with 74 members across 5 generations.
Medicine96(11)e6249(2017 Mar)
Guo X,Fan C,Wang Y,Wang M,Cai C,Yang Y,Zhao S,Duan F,Li Y


book

Spectrum of Mutations in Hypertrophic Cardiomyopathy Genes Among Tunisian Patients.
Genetic testing and molecular biomarkers20(11)674-679(2016 Nov)
Jaafar N,Gómez J,Kammoun I,Zairi I,Amara WB,Kachboura S,Kraiem S,Hammami M,Iglesias S,Alonso B,Coto E


book

Two novel MYH7 proline substitutions cause Laing Distal Myopathy-like phenotypes with variable expressivity and neck extensor contracture.
BMC medical genetics17(1)57(2016 Aug)
Feinstein-Linial M,Buvoli M,Buvoli A,Sadeh M,Dabby R,Straussberg R,Shelef I,Dayan D,Leinwand LA,Birk OS


 
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