×

MYL2 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MYL2
Entrez Gene ID 4633
Full Name myosin light chain 2
Synonyms CMH10,MLC-2s/v,MLC2
General protein information
Preferred Names

myosin light chain 2

Names

myosin regulatory light chain 2, ventricular/cardiac muscle isoform
MLC-2
MLC-2v
RLC of myosin
cardiac myosin light chain 2
cardiac ventricular myosin light chain 2
myosin light chain 2, slow skeletal/ventricular muscle isoform
myosin, light chain 2, regulatory, cardiac, slow
myosin, light polypeptide 2, regulatory, cardiac, slow
regulatory light chain of myosin
slow cardiac myosin regulatory light chain 2
ventricular myosin light chain 2

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

12

12q24.11

Summary Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008].
Disorder MIM:

160781

Disorder Html: Cardiomyopathy, familial hypertrophic, 10, 608758 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_000432.3 NP_000423.2 myosin regulatory light chain 2, ventricular/cardiac muscle isoform



Rattus norvegicus (Norway rat) Myl2 NP_001030329.2
Gallus gallus (chicken) MYL2 NP_001258858.1
Canis lupus familiaris (dog) MYL2 NP_001003069.1
Danio rerio (zebrafish) myl2b NP_001035134.1
Danio rerio (zebrafish) LOC100537244 XP_003198611.2
Xenopus tropicalis (tropical clawed frog) myl2 NP_001107717.1
Pan troglodytes (chimpanzee) MYL2 XP_001144828.2
Mus musculus (house mouse) Myl2 NP_034991.3
Bos taurus (cattle) MYL2 NP_001030197.1
Homo sapiens (human) MYL2 NP_000423.2
Macaca mulatta (rhesus monkey) MYL2 XP_001100796.1


Related articles in PubMed

A Potential Oligogenic Etiology of Hypertrophic Cardiomyopathy: A Classic Single-Gene Disorder.
Li L, Bainbridge MN, Tan Y, Willerson JT, Marian AJ
Circulation research120(7)1084-1090(2017 Mar)

Effect of obesity on the association between MYL2 (rs3782889) and high-density lipoprotein cholesterol among Korean men.
Cho ER, Jee YH, Kim SW, Sull JW
Journal of human genetics61(5)405-9(2016 May)

Functions of myosin light chain-2 (MYL2) in cardiac muscle and disease.
Sheikh F, Lyon RC, Chen J
Gene569(1)14-20(2015 Sep)

Myosin regulatory light chain phosphorylation enhances cardiac β-myosin in vitro motility under load.
Karabina A, Kazmierczak K, Szczesna-Cordary D, Moore JR
Archives of biochemistry and biophysics58014-21(2015 Aug)

Filamin B Enhances the Invasiveness of Cancer Cells into 3D Collagen Matrices.
Iguchi Y, Ishihara S, Uchida Y, Tajima K, Mizutani T, Kawabata K, Haga H
Cell structure and function40(2)61-7(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Structural dynamics-based approach reveals that the E56G mutation in human ventricular essential light chain affects the structure of the actin-myosin complex in the presence of ATP. The mutation increases the population in the S structural state (increasing the duty ratio), and changes the structure of the W state, so that it more closely resembles the S state.
Title: A Cardiomyopathy Mutation in the Myosin Essential Light Chain Alters Actomyosin Structure.

Lipolysis-stimulated lipoprotein receptors (LSRs) localized to bicellular junctions in association with myosin regulatory light chain 2 (MRLC2) at low cell densities and to tricellular contacts when myosin phosphatase target subunit 1 (MYPT1) localized to the bicellular regions.
Title: The bicellular tensile force sorts the localization of LSRs in bicellular and tricellular junctions.

Two siblings with hypertrophic cardiomyopathy had the pathogenic variant p.Ala13Thr variant in MYL2.
Title: A Potential Oligogenic Etiology of Hypertrophic Cardiomyopathy: A Classic Single-Gene Disorder.

the MYL2 gene on chromosome 12 is associated with serum HDL-C levels in Korean men. The association was much stronger in male obese subjects and smokers than that in leaner nonsmoking male subjects.
Title: Effect of obesity on the association between MYL2 (rs3782889) and high-density lipoprotein cholesterol among Korean men.

Myosin regulatory light chain phosphorylation enhances cardiac beta-myosin in vitro motility under load.
Title: Myosin regulatory light chain phosphorylation enhances cardiac β-myosin in vitro motility under load.

The following MYL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18354 NM_000432.3
Latest version!
Homo sapiens myosin light chain 2 (MYL2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18354
Clone ID Related Accession (Same CDS sequence) NM_000432.3
Accession Version NM_000432.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 501bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA090235.1, BC015821.1 and BC031008.1. This sequence is a reference standard in the RefSeqGene project. On Jun 14, 2008 this sequence version replaced NM_000432.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279847.4406.1, BC031008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
ATGGCACCTA AGAAAGCAAA GAAGAGAGCC GGGGGCGCCA ACTCCAACGT GTTCTCCATG 
TTCGAACAGA CCCAAATCCA GGAATTTAAG GAGGCCTTCA CTATCATGGA CCAGAACAGG
GATGGCTTCA TTGACAAGAA CGATCTGAGA GACACCTTTG CTGCCCTTGG GCGAGTGAAC
GTGAAAAATG AAGAAATTGA TGAAATGATC AAGGAGGCTC CGGGTCCAAT TAACTTTACT
GTGTTCCTCA CAATGTTTGG GGAGAAACTT AAGGGAGCGG ACCCTGAGGA AACCATTCTC
AACGCATTCA AAGTGTTTGA CCCTGAAGGC AAAGGGGTGC TGAAGGCTGA TTACGTTCGG
GAAATGCTGA CCACGCAGGC GGAGAGGTTT TCCAAGGAGG AGGTTGACCA GATGTTCGCC
GCCTTCCCCC CTGACGTGAC TGGCAACTTG GACTACAAGA ACCTGGTGCA CATCATCACC
CACGGAGAAG AGAAGGACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000423.2
CDS72..572
Misc Feature(1)75..77
Misc Feature(2)111..113
Misc Feature(3)114..560
Misc Feature(4)114..116
Misc Feature(5)126..128
Misc Feature(6)126..128
Misc Feature(7)153..>290
Misc Feature(8)225..227
Misc Feature(9)375..551
Misc Feature(10)order(390..392,396..398,402..404,423..425,498..500,504..506,510..512,531..533)
Exon (1)1..74
Exon (2)75..164
Exon (3)165..240
Exon (4)241..345
Exon (5)346..424
Exon (6)425..473
Exon (7)474..830
Translation

Target ORF information:

RefSeq Version NM_000432.3
Organism Homo sapiens(Human)
Definition Homo sapiens myosin light chain 2 (MYL2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000432.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGGCACCTA AGAAAGCAAA GAAGAGAGCC GGGGGCGCCA ACTCCAACGT GTTCTCCATG 
TTCGAACAGA CCCAAATCCA GGAATTTAAG GAGGCCTTCA CTATCATGGA CCAGAACAGG
GATGGCTTCA TTGACAAGAA CGATCTGAGA GACACCTTTG CTGCCCTTGG GCGAGTGAAC
GTGAAAAATG AAGAAATTGA TGAAATGATC AAGGAGGCTC CGGGTCCAAT TAACTTTACT
GTGTTCCTCA CAATGTTTGG GGAGAAACTT AAGGGAGCGG ACCCTGAGGA AACCATTCTC
AACGCATTCA AAGTGTTTGA CCCTGAAGGC AAAGGGGTGC TGAAGGCTGA TTACGTTCGG
GAAATGCTGA CCACGCAGGC GGAGAGGTTT TCCAAGGAGG AGGTTGACCA GATGTTCGCC
GCCTTCCCCC CTGACGTGAC TGGCAACTTG GACTACAAGA ACCTGGTGCA CATCATCACC
CACGGAGAAG AGAAGGACTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A Potential Oligogenic Etiology of Hypertrophic Cardiomyopathy: A Classic Single-Gene Disorder.
Circulation research120(7)1084-1090(2017 Mar)
Li L,Bainbridge MN,Tan Y,Willerson JT,Marian AJ


book

Effect of obesity on the association between MYL2 (rs3782889) and high-density lipoprotein cholesterol among Korean men.
Journal of human genetics61(5)405-9(2016 May)
Cho ER,Jee YH,Kim SW,Sull JW


book

Functions of myosin light chain-2 (MYL2) in cardiac muscle and disease.
Gene569(1)14-20(2015 Sep)
Sheikh F,Lyon RC,Chen J


book

Myosin regulatory light chain phosphorylation enhances cardiac β-myosin in vitro motility under load.
Archives of biochemistry and biophysics58014-21(2015 Aug)
Karabina A,Kazmierczak K,Szczesna-Cordary D,Moore JR


book

Filamin B Enhances the Invasiveness of Cancer Cells into 3D Collagen Matrices.
Cell structure and function40(2)61-7(2015)
Iguchi Y,Ishihara S,Uchida Y,Tajima K,Mizutani T,Kawabata K,Haga H


 
*
*
*
*