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MYL4 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol MYL4
Entrez Gene ID 4635
Full Name myosin light chain 4
Synonyms ALC1,AMLC,GT1,PRO1957
General protein information
Preferred Names

myosin light chain 4

Names

myosin light chain 4
myosin light chain 1, embryonic muscle/atrial isoform
myosin light chain alkali GT-1 isoform
myosin, atrial/fetal muscle, light chain
myosin, light chain 4, alkali; atrial, embryonic
myosin, light polypeptide 4, alkali; atrial, embryonic

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

17

17q21.32

Summary Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:

160770


mRNA and Protein(s)

mRNA Protein Name
NM_002476.2 NP_002467.1 myosin light chain 4
XM_011524838.1 XP_011523140.1 myosin light chain 4 isoform X1
XM_005257391.3 XP_005257448.1 myosin light chain 4 isoform X1
XM_005257391.4 XP_005257448.1 myosin light chain 4 isoform X2
NM_001002841.1 NP_001002841.1 myosin light chain 4
XM_011524839.2 XP_011523141.2 myosin light chain 4 isoform X1
XM_017024683.1 XP_016880172.1 myosin light chain 4 isoform X1



Rattus norvegicus (Norway rat) Myl4 NP_001102965.1
Gallus gallus (chicken) MYL4 XP_003642828.1
Danio rerio (zebrafish) myl4 NP_001020354.1
Macaca mulatta (rhesus monkey) LOC717354 XP_001116001.1
Mus musculus (house mouse) Myl4 NP_034988.2
Homo sapiens (human) MYL4 NP_002467.1
Canis lupus familiaris (dog) MYL4 XP_005624249.1
Xenopus tropicalis (tropical clawed frog) myl4 NP_001096232.1
Pan troglodytes (chimpanzee) MYL4 XP_511623.1
Bos taurus (cattle) MYL4 NP_001068617.1


Related articles in PubMed

A mutation in the atrial-specific myosin light chain gene (MYL4) causes familial atrial fibrillation.
Orr N, Arnaout R, Gula LJ, Spears DA, Leong-Sit P, Li Q, Tarhuni W, Reischauer S, Chauhan VS, Borkovich M, Uppal S, Adler A, Coughlin SR, Stainier DY, Gollob MH
Nature communications711303(2016 Apr)

Large-scale whole-genome sequencing of the Icelandic population.
Gudbjartsson DF, Helgason H, Gudjonsson SA, Zink F, Oddson A, Gylfason A, Besenbacher S, Magnusson G, Halldorsson BV, Hjartarson E, Sigurdsson GT, Stacey SN, Frigge ML, Holm H, Saemundsdottir J, Helgadottir HT, Johannsdottir H, Sigfusson G, Thorgeirsson G, Sverrisson JT, Gretarsdottir S, Walters GB, Rafnar T, Thjodleifsson B, Bjornsson ES, Olafsson S, Thorarinsdottir H, Steingrimsdottir T, Gudmundsdottir TS, Theodors A, Jonasson JG, Sigurdsson A, Bjornsdottir G, Jonsson JJ, Thorarensen O, Ludvigsson P, Gudbjartsson H, Eyjolfsson GI, Sigurdardottir O, Olafsson I, Arnar DO, Magnusson OT, Kong A, Masson G, Thorsteinsdottir U, Helgason A, Sulem P, Stefansson K
Nature genetics47(5)435-44(2015 May)

Minigenes encoding N-terminal domains of human cardiac myosin light chain-1 improve heart function of transgenic rats.
Haase H, Dobbernack G, Tünnemann G, Karczewski P, Cardoso C, Petzhold D, Schlegel WP, Lutter S, Pierschalek P, Behlke J, Morano I
FASEB journal : official publication of the Federation of American Societies for Experimental Biology20(7)865-73(2006 May)

Human atrial myosin light chain 1 expression attenuates heart failure.
Abdelaziz AI, Pagel I, Schlegel WP, Kott M, Monti J, Haase H, Morano I
Advances in experimental medicine and biology565283-92; discussion 92, 405-15(2005)

Functional characterization of the human atrial essential myosin light chain (hALC-1) in a transgenic rat model.
Abdelaziz AI, Segaric J, Bartsch H, Petzhold D, Schlegel WP, Kott M, Seefeldt I, Klose J, Bader M, Haase H, Morano I
Journal of molecular medicine (Berlin, Germany)82(4)265-74(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a novel, heterozygous p.Glu11Lys mutation in the atrial-specific myosin light chain gene MYL4, caused atrial fibrillation.
Title: A mutation in the atrial-specific myosin light chain gene (MYL4) causes familial atrial fibrillation.

A recessive frameshift mutation in MYL4 causes early-onset atrial fibrillation.
Title: Large-scale whole-genome sequencing of the Icelandic population.

results demonstrate that the expression of hALC-1 could have a beneficial effect on the overloaded hypertrophied heart
Title: Human atrial myosin light chain 1 expression attenuates heart failure.

NMR showed involvement of N-terminus and lysines 3 and 4 in interaction with actin. Mutations of these lysines (K3A,K4R,K4A,K4D) resulted in altered actin binding and actin activated MgATPase. Mutation of Ala-1 to val had no effect on binding or kinetics.
Title: Size and charge requirements for kinetic modulation and actin binding by alkali 1-type myosin essential light chains.

The N-terminus of this protein is an actin binding site.
Title: The N-terminus of A1-type myosin essential light chains binds actin and modulates myosin motor function.

The following MYL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu30031 NM_001002841.1
Latest version!
Homo sapiens myosin light chain 4 (MYL4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu30031 NM_002476.2
Latest version!
Homo sapiens myosin light chain 4 (MYL4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu30031 XM_005257391.4
Latest version!
Homo sapiens myosin light chain 4 (MYL4), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu30031 XM_011524838.1
Latest version!
Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu86891 XM_017024683.1
Latest version!
Homo sapiens myosin light chain 4 (MYL4), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
OHu86891 XM_011524839.2
Latest version!
Homo sapiens myosin light chain 4 (MYL4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00
View Old Accession Versions >>
OHu30031 XM_005257391.3 Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu30031
Clone ID Related Accession (Same CDS sequence) NM_002476.2 , XM_011524838.1 , XM_005257391.3 , XM_005257391.4 , NM_001002841.1
Accession Version NM_002476.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-25
Organism Homo sapiens(Human)
Product myosin light chain 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X58851.1. On Dec 18, 2001 this sequence version replaced NM_002476.1. Transcript Variant: This variant (2) lacks the 5' exon but has an alternate 5' end, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC030228.1, BI835446.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

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ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_002467.1
CDS76..669
Misc Feature(1)79..81
Misc Feature(2)220..663
Misc Feature(3)271..273
Misc Feature(4)295..297
Misc Feature(5)343..345
Misc Feature(6)475..660
Misc Feature(7)order(502..504,508..510,514..516,535..537,607..609,613..615,619..621,640..642)
Misc Feature(8)661..663
Exon (1)1..210
Exon (2)211..238
Exon (3)239..388
Exon (4)389..562
Exon (5)563..640
Exon (6)641..684
Exon (7)685..855
Translation

Target ORF information:

RefSeq Version NM_002476.2
Organism Homo sapiens(Human)
Definition Homo sapiens myosin light chain 4 (MYL4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_002476.2

ORF Insert Sequence:

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ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu30031
Clone ID Related Accession (Same CDS sequence) NM_002476.2 , XM_011524838.1 , XM_005257391.3 , XM_005257391.4 , NM_001002841.1
Accession Version NM_001002841.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-26
Organism Homo sapiens(Human)
Product myosin light chain 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from H83803.1, BU658678.1 and BC030228.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longer transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC030228.1, ERR279855.1482.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

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ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001002841.1
CDS129..722
Misc Feature(1)90..92
Misc Feature(2)132..134
Misc Feature(3)273..716
Misc Feature(4)324..326
Misc Feature(5)348..350
Misc Feature(6)396..398
Misc Feature(7)528..713
Misc Feature(8)order(555..557,561..563,567..569,588..590,660..662,666..668,672..674,693..695)
Misc Feature(9)714..716
Exon (1)1..94
Exon (2)95..263
Exon (3)264..291
Exon (4)292..441
Exon (5)442..615
Exon (6)616..693
Exon (7)694..737
Exon (8)738..908
Translation

Target ORF information:

RefSeq Version NM_001002841.1
Organism Homo sapiens(Human)
Definition Homo sapiens myosin light chain 4 (MYL4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001002841.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu30031
Clone ID Related Accession (Same CDS sequence) NM_002476.2 , XM_011524838.1 , XM_005257391.3 , XM_005257391.4 , NM_001002841.1
Accession Version XM_011524838.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product myosin light chain 4 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523140.1
Translation

Target ORF information:

RefSeq Version XM_011524838.1
Organism Homo sapiens(Human)
Definition Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524838.1

ORF Insert Sequence:

1
61
121
181
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301
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421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu30031
Clone ID Related Accession (Same CDS sequence) NM_002476.2 , XM_011524838.1 , XM_005257391.3 , XM_005257391.4 , NM_001002841.1
Accession Version XM_005257391.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product myosin light chain 4 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257448.1
Translation

Target ORF information:

RefSeq Version XM_005257391.3
Organism Homo sapiens(Human)
Definition Homo sapiens myosin, light chain 4, alkali; atrial, embryonic (MYL4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257391.3

ORF Insert Sequence:

1
61
121
181
241
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481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu30031
Clone ID Related Accession (Same CDS sequence) NM_002476.2 , XM_011524838.1 , XM_005257391.3 , XM_005257391.4 , NM_001002841.1
Accession Version XM_005257391.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 594bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product myosin light chain 4 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257391.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257448.1
CDS56..649
Misc Feature(1)200..643
Misc Feature(2)455..640
Misc Feature(3)order(482..484,488..490,494..496,515..517,587..589,593..595,599..601,620..622)
Translation

Target ORF information:

RefSeq Version XM_005257391.4
Organism Homo sapiens(Human)
Definition Homo sapiens myosin light chain 4 (MYL4), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257391.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGAGTTCAA AGAGGCCTTT
TCATTGTTTG ACCGGACCCC GACTGGAGAG ATGAAGATCA CCTACGGCCA GTGCGGGGAT
GTACTGCGGG CCCTGGGCCA GAACCCTACC AATGCCGAGG TGCTGCGTGT GCTGGGCAAG
CCCAAGCCTG AAGAGATGAA TGTCAAGATG CTGGACTTTG AGACGTTCTT GCCCATCCTG
CAGCACATTT CCCGCAACAA GGAGCAGGGC ACCTATGAGG ACTTCGTGGA GGGCCTGCGT
GTCTTTGACA AGGAGAGCAA TGGCACGGTC ATGGGTGCTG AGCTTCGGCA CGTCCTTGCC
ACCCTGGGAG AGAAGATGAC TGAGGCTGAA GTGGAGCAGC TGTTAGCTGG GCAAGAGGAT
GCCAATGGCT GCATCAATTA TGAAGCCTTT GTCAAGCACA TCATGTCAGG GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86891
Clone ID Related Accession (Same CDS sequence) XM_011524839.2 , XM_017024683.1
Accession Version XM_011524839.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 687bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product myosin light chain 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011524839.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGGTGAGTA TGGACAACCC
CACCTCTCCG TCTCTATTGC ACTTTCCTGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAGG
AGTAGTTGTC CTCATGAGTT CAAAGAGGCC TTTTCATTGT TTGACCGGAC CCCGACTGGA
GAGATGAAGA TCACCTACGG CCAGTGCGGG GATGTACTGC GGGCCCTGGG CCAGAACCCT
ACCAATGCCG AGGTGCTGCG TGTGCTGGGC AAGCCCAAGC CTGAAGAGAT GAATGTCAAG
ATGCTGGACT TTGAGACGTT CTTGCCCATC CTGCAGCACA TTTCCCGCAA CAAGGAGCAG
GGCACCTATG AGGACTTCGT GGAGGGCCTG CGTGTCTTTG ACAAGGAGAG CAATGGCACG
GTCATGGGTG CTGAGCTTCG GCACGTCCTT GCCACCCTGG GAGAGAAGAT GACTGAGGCT
GAAGTGGAGC AGCTGTTAGC TGGGCAAGAG GATGCCAATG GCTGCATCAA TTATGAAGCC
TTTGTCAAGC ACATCATGTC AGGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011523141.2
CDS123..809
Translation

Target ORF information:

RefSeq Version XM_011524839.2
Organism Homo sapiens(Human)
Definition Homo sapiens myosin light chain 4 (MYL4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011524839.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGGTGAGTA TGGACAACCC
CACCTCTCCG TCTCTATTGC ACTTTCCTGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAGG
AGTAGTTGTC CTCATGAGTT CAAAGAGGCC TTTTCATTGT TTGACCGGAC CCCGACTGGA
GAGATGAAGA TCACCTACGG CCAGTGCGGG GATGTACTGC GGGCCCTGGG CCAGAACCCT
ACCAATGCCG AGGTGCTGCG TGTGCTGGGC AAGCCCAAGC CTGAAGAGAT GAATGTCAAG
ATGCTGGACT TTGAGACGTT CTTGCCCATC CTGCAGCACA TTTCCCGCAA CAAGGAGCAG
GGCACCTATG AGGACTTCGT GGAGGGCCTG CGTGTCTTTG ACAAGGAGAG CAATGGCACG
GTCATGGGTG CTGAGCTTCG GCACGTCCTT GCCACCCTGG GAGAGAAGAT GACTGAGGCT
GAAGTGGAGC AGCTGTTAGC TGGGCAAGAG GATGCCAATG GCTGCATCAA TTATGAAGCC
TTTGTCAAGC ACATCATGTC AGGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu86891
Clone ID Related Accession (Same CDS sequence) XM_011524839.2 , XM_017024683.1
Accession Version XM_017024683.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 687bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product myosin light chain 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGGTGAGTA TGGACAACCC
CACCTCTCCG TCTCTATTGC ACTTTCCTGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAGG
AGTAGTTGTC CTCATGAGTT CAAAGAGGCC TTTTCATTGT TTGACCGGAC CCCGACTGGA
GAGATGAAGA TCACCTACGG CCAGTGCGGG GATGTACTGC GGGCCCTGGG CCAGAACCCT
ACCAATGCCG AGGTGCTGCG TGTGCTGGGC AAGCCCAAGC CTGAAGAGAT GAATGTCAAG
ATGCTGGACT TTGAGACGTT CTTGCCCATC CTGCAGCACA TTTCCCGCAA CAAGGAGCAG
GGCACCTATG AGGACTTCGT GGAGGGCCTG CGTGTCTTTG ACAAGGAGAG CAATGGCACG
GTCATGGGTG CTGAGCTTCG GCACGTCCTT GCCACCCTGG GAGAGAAGAT GACTGAGGCT
GAAGTGGAGC AGCTGTTAGC TGGGCAAGAG GATGCCAATG GCTGCATCAA TTATGAAGCC
TTTGTCAAGC ACATCATGTC AGGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016880172.1
CDS110..796
Translation

Target ORF information:

RefSeq Version XM_017024683.1
Organism Homo sapiens(Human)
Definition Homo sapiens myosin light chain 4 (MYL4), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017024683.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGGCTCCCA AGAAGCCTGA GCCTAAGAAG GAGGCAGCCA AGCCAGCTCC AGCTCCAGCT 
CCAGCCCCTG CACCAGCCCC TGCCCCAGCT CCTGAGGCTC CCAAGGAACC TGCCTTTGAC
CCCAAGAGTG TAAAGATAGA CTTCACTGCC GACCAGATTG AAGGTGAGTA TGGACAACCC
CACCTCTCCG TCTCTATTGC ACTTTCCTGG AGGAGGAGGA GGAGGAGGAA GAAGAGGAGG
AGTAGTTGTC CTCATGAGTT CAAAGAGGCC TTTTCATTGT TTGACCGGAC CCCGACTGGA
GAGATGAAGA TCACCTACGG CCAGTGCGGG GATGTACTGC GGGCCCTGGG CCAGAACCCT
ACCAATGCCG AGGTGCTGCG TGTGCTGGGC AAGCCCAAGC CTGAAGAGAT GAATGTCAAG
ATGCTGGACT TTGAGACGTT CTTGCCCATC CTGCAGCACA TTTCCCGCAA CAAGGAGCAG
GGCACCTATG AGGACTTCGT GGAGGGCCTG CGTGTCTTTG ACAAGGAGAG CAATGGCACG
GTCATGGGTG CTGAGCTTCG GCACGTCCTT GCCACCCTGG GAGAGAAGAT GACTGAGGCT
GAAGTGGAGC AGCTGTTAGC TGGGCAAGAG GATGCCAATG GCTGCATCAA TTATGAAGCC
TTTGTCAAGC ACATCATGTC AGGGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

A mutation in the atrial-specific myosin light chain gene (MYL4) causes familial atrial fibrillation.
Nature communications711303(2016 Apr)
Orr N,Arnaout R,Gula LJ,Spears DA,Leong-Sit P,Li Q,Tarhuni W,Reischauer S,Chauhan VS,Borkovich M,Uppal S,Adler A,Coughlin SR,Stainier DY,Gollob MH


book

Large-scale whole-genome sequencing of the Icelandic population.
Nature genetics47(5)435-44(2015 May)
Gudbjartsson DF,Helgason H,Gudjonsson SA,Zink F,Oddson A,Gylfason A,Besenbacher S,Magnusson G,Halldorsson BV,Hjartarson E,Sigurdsson GT,Stacey SN,Frigge ML,Holm H,Saemundsdottir J,Helgadottir HT,Johannsdottir H,Sigfusson G,Thorgeirsson G,Sverrisson JT,Gretarsdottir S,Walters GB,Rafnar T,Thjodleifsson B,Bjornsson ES,Olafsson S,Thorarinsdottir H,Steingrimsdottir T,Gudmundsdottir TS,Theodors A,Jonasson JG,Sigurdsson A,Bjornsdottir G,Jonsson JJ,Thorarensen O,Ludvigsson P,Gudbjartsson H,Eyjolfsson GI,Sigurdardottir O,Olafsson I,Arnar DO,Magnusson OT,Kong A,Masson G,Thorsteinsdottir U,Helgason A,Sulem P,Stefansson K


book

Minigenes encoding N-terminal domains of human cardiac myosin light chain-1 improve heart function of transgenic rats.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology20(7)865-73(2006 May)
Haase H,Dobbernack G,Tünnemann G,Karczewski P,Cardoso C,Petzhold D,Schlegel WP,Lutter S,Pierschalek P,Behlke J,Morano I


book

Human atrial myosin light chain 1 expression attenuates heart failure.
Advances in experimental medicine and biology565283-92; discussion 92, 405-15(2005)
Abdelaziz AI,Pagel I,Schlegel WP,Kott M,Monti J,Haase H,Morano I


book

Functional characterization of the human atrial essential myosin light chain (hALC-1) in a transgenic rat model.
Journal of molecular medicine (Berlin, Germany)82(4)265-74(2004 Apr)
Abdelaziz AI,Segaric J,Bartsch H,Petzhold D,Schlegel WP,Kott M,Seefeldt I,Klose J,Bader M,Haase H,Morano I


 
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