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NCAM1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol NCAM1
Entrez Gene ID 4684
Full Name neural cell adhesion molecule 1
Synonyms CD56, MSK39, NCAM
General protein information
Preferred Names
neural cell adhesion molecule 1
Names
neural cell adhesion molecule 1
neural cell adhesion molecule, NCAM
antigen recognized by monoclonal antibody 5.1H11
NP_000606.3
NP_001070150.1
NP_001229536.1
NP_001229537.1
NP_851996.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q23.1

Summary This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]. lac of sum
Disorder MIM:

116930


mRNA and Protein(s)

mRNA Protein Name
NM_000615 NP_000606 neural cell adhesion molecule 1 isoform 1 precursor
NM_181351 NP_851996 neural cell adhesion molecule 1 isoform 2 precursor
NM_001076682 NP_001070150 neural cell adhesion molecule 1 isoform 3 precursor
NM_001242607 NP_001229536 neural cell adhesion molecule 1 isoform 5 precursor
NM_001242608 NP_001229537 neural cell adhesion molecule 1 isoform 4 precursor



Homo sapiens (human) NCAM1 NP_001229536.1
Pan troglodytes (chimpanzee) NCAM1 XP_003952087.1
Macaca mulatta (Rhesus monkey) NCAM1 XP_001083366.1
Canis lupus familiaris (dog) NCAM1 NP_001010950.1
Bos taurus (cattle) NCAM1 NP_776824.1
Mus musculus (house mouse) Ncam1 NP_001106675.1
Rattus norvegicus (Norway rat) Ncam1 NP_113709.1
Gallus gallus (chicken) NCAM1 NP_001122300.2
Danio rerio (zebrafish) ncam1a NP_571277.1
Drosophila melanogaster (fruit fly) Fas2 NP_525066.1
Caenorhabditis elegans ncam-1 NP_741709.1
Xenopus (Silurana) tropicalis (western clawed frog) ncam1 NP_001090869.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCAM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCAM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu00262
NM_000615 Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$339.50-$475.30
$679.00
OHu20922 NM_181351 Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
OHu00364 NM_001076682 Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $538.30
$769.00
OHu00345 NM_001242607 Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $475.30
$679.00
OHu00771 NM_001242608 Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu00262
Clone ID Related Accession (Same CDS sequence) NM_000615
Accession Version NM_000615.6 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2547bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neural cell adhesion molecule 1 isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KF511120.1, DA337649.1, BC047244.1, S71824.1, BC014205.2, AK054570.1 and AW131780.1. On Jun 17, 2011 this sequence version replaced gi:117320538. Transcript Variant: This variant (1) lacks two alternate in-frame exons in the 3' coding region compared to variant 5. The resulting protein (isoform 1) is shorter compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S71824.1, U63041.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGA CTCTGGATGG GCACATGGTG
GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG ACCCTGAAGA GCATCCAGTA CACTGATGCC
GGAGAGTACA TCTGCACCGC CAGCAACACC ATCGGCCAGG ACTCCCAGTC CATGTACCTT
GAAGTGCAAT ATGCCCCAAA GCTACAGGGC CCTGTGGCTG TGTACACTTG GGAGGGGAAC
CAGGTGAACA TCACCTGCGA GGTATTTGCC TATCCCAGTG CCACGATCTC ATGGTTTCGG
GATGGCCAGC TGCTGCCAAG CTCCAATTAC AGCAATATCA AGATCTACAA CACCCCCTCT
GCCAGCTATC TGGAGGTGAC CCCAGACTCT GAGAATGATT TTGGGAACTA CAACTGTACT
GCAGTGAACC GCATTGGGCA GGAGTCCTTG GAATTCATCC TTGTTCAAGC AGACACCCCC
TCTTCACCAT CCATCGACCA GGTGGAGCCA TACTCCAGCA CAGCCCAGGT GCAGTTTGAT
GAACCAGAGG CCACAGGTGG GGTGCCCATC CTCAAATACA AAGCTGAGTG GAGAGCAGTT
GGTGAAGAAG TATGGCATTC CAAGTGGTAT GATGCCAAGG AAGCCAGCAT GGAGGGCATC
GTCACCATCG TGGGCCTGAA GCCCGAAACA ACGTACGCCG TAAGGCTGGC GGCGCTCAAT
GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC TCCGAGTTCA AGACGCAGCC AGTCCAAGGG
GAACCCAGTG CACCTAAGCT CGAAGGGCAG ATGGGAGAGG ATGGAAACTC TATTAAAGTG
AACCTGATCA AGCAGGATGA CGGCGGCTCC CCCATCAGAC ACTATCTGGT CAGGTACCGA
GCGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
CTGAAGTCCC TGGACTGGAA TGCTGAGTAT GAGGTCTACG TGGTGGCTGA GAACCAGCAA
GGAAAATCCA AGGCGGCTCA TTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
GCCAACGGCA GCCCCACCTC AGGCCTGAGC ACCGGGGCCA TCGTGGGCAT CCTCATCGTC
ATCTTCGTCC TGCTCCTGGT GGTTGTGGAC ATCACCTGCT ACTTCCTGAA CAAGTGTGGC
CTGTTCATGT GCATTGCGGT CAACCTGTGT GGAAAAGCCG GGCCCGGGGC CAAGGGCAAG
GACATGGAGG AGGGCAAGGC CGCCTTCTCG AAAGATGAGT CCAAGGAGCC CATCGTGGAG
GTTCGAACGG AGGAGGAGAG GACCCCAAAC CATGATGGAG GGAAACACAC AGAGCCCAAC
GAGACCACGC CACTGACGGA GCCCGAGAAG GGCCCCGTAG AAGCAAAGCC AGAGTGCCAG
GAGACAGAAA CGAAGCCAGC GCCAGCCGAA GTCAAGACGG TCCCCAATGA CGCCACACAG
ACAAAGGAGA ACGAGAGCAA AGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000606.3
CDS367..2913
Misc Feature(1)424..711(+)
Misc Feature(2)427..702(+)
Misc Feature(3)469..480(+)
Misc Feature(4)757..942(+)
Misc Feature(5)769..936(+)
Misc Feature(6)997..1287(+)
Misc Feature(7)1030..1278(+)
Misc Feature(8)1282..1572(+)
Misc Feature(9)1333..1542(+)
Misc Feature(10)1597..1833(+)
Misc Feature(11)1630..1842(+)
Misc Feature(12)1711..1713(+)
Misc Feature(13)1798..1800(+)
Misc Feature(14)1858..2151(+)
Misc Feature(15)2068..2115(+)
Misc Feature(16)2116..2130(+)
Misc Feature(17)2212..2442(+)
Misc Feature(18)2362..2409(+)
Misc Feature(19)2410..2424(+)
Misc Feature(20)2491..2553(+)
Exon (1)1..418
Gene:NCAM1
Gene Synonym:
Exon (2)419..493
Gene:NCAM1
Gene Synonym:
Exon (3)494..712
Gene:NCAM1
Gene Synonym:
Exon (4)713..856
Gene:NCAM1
Gene Synonym:
Exon (5)857..994
Gene:NCAM1
Gene Synonym:
Exon (6)995..1112
Gene:NCAM1
Gene Synonym:
Exon (7)1113..1282
Gene:NCAM1
Gene Synonym:
Exon (8)1283..1425
Gene:NCAM1
Gene Synonym:
Exon (9)1426..1576
Gene:NCAM1
Gene Synonym:
Exon (10)1577..1761
Gene:NCAM1
Gene Synonym:
Exon (11)1762..1858
Gene:NCAM1
Gene Synonym:
Exon (12)1859..2029
Gene:NCAM1
Gene Synonym:
Exon (13)2030..2161
Gene:NCAM1
Gene Synonym:
Exon (14)2162..2164
Gene:NCAM1
Gene Synonym:
Exon (15)2165..2289
Gene:NCAM1
Gene Synonym:
Exon (16)2290..2467
Gene:NCAM1
Gene Synonym:
Exon (17)2468..2675
Gene:NCAM1
Gene Synonym:
Exon (18)2676..2792
Gene:NCAM1
Gene Synonym:
Exon (19)2793..5962
Gene:NCAM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000615
Organism Homo sapiens (human)
Definition Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000615

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGA CTCTGGATGG GCACATGGTG
GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG ACCCTGAAGA GCATCCAGTA CACTGATGCC
GGAGAGTACA TCTGCACCGC CAGCAACACC ATCGGCCAGG ACTCCCAGTC CATGTACCTT
GAAGTGCAAT ATGCCCCAAA GCTACAGGGC CCTGTGGCTG TGTACACTTG GGAGGGGAAC
CAGGTGAACA TCACCTGCGA GGTATTTGCC TATCCCAGTG CCACGATCTC ATGGTTTCGG
GATGGCCAGC TGCTGCCAAG CTCCAATTAC AGCAATATCA AGATCTACAA CACCCCCTCT
GCCAGCTATC TGGAGGTGAC CCCAGACTCT GAGAATGATT TTGGGAACTA CAACTGTACT
GCAGTGAACC GCATTGGGCA GGAGTCCTTG GAATTCATCC TTGTTCAAGC AGACACCCCC
TCTTCACCAT CCATCGACCA GGTGGAGCCA TACTCCAGCA CAGCCCAGGT GCAGTTTGAT
GAACCAGAGG CCACAGGTGG GGTGCCCATC CTCAAATACA AAGCTGAGTG GAGAGCAGTT
GGTGAAGAAG TATGGCATTC CAAGTGGTAT GATGCCAAGG AAGCCAGCAT GGAGGGCATC
GTCACCATCG TGGGCCTGAA GCCCGAAACA ACGTACGCCG TAAGGCTGGC GGCGCTCAAT
GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC TCCGAGTTCA AGACGCAGCC AGTCCAAGGG
GAACCCAGTG CACCTAAGCT CGAAGGGCAG ATGGGAGAGG ATGGAAACTC TATTAAAGTG
AACCTGATCA AGCAGGATGA CGGCGGCTCC CCCATCAGAC ACTATCTGGT CAGGTACCGA
GCGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
CTGAAGTCCC TGGACTGGAA TGCTGAGTAT GAGGTCTACG TGGTGGCTGA GAACCAGCAA
GGAAAATCCA AGGCGGCTCA TTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
GCCAACGGCA GCCCCACCTC AGGCCTGAGC ACCGGGGCCA TCGTGGGCAT CCTCATCGTC
ATCTTCGTCC TGCTCCTGGT GGTTGTGGAC ATCACCTGCT ACTTCCTGAA CAAGTGTGGC
CTGTTCATGT GCATTGCGGT CAACCTGTGT GGAAAAGCCG GGCCCGGGGC CAAGGGCAAG
GACATGGAGG AGGGCAAGGC CGCCTTCTCG AAAGATGAGT CCAAGGAGCC CATCGTGGAG
GTTCGAACGG AGGAGGAGAG GACCCCAAAC CATGATGGAG GGAAACACAC AGAGCCCAAC
GAGACCACGC CACTGACGGA GCCCGAGAAG GGCCCCGTAG AAGCAAAGCC AGAGTGCCAG
GAGACAGAAA CGAAGCCAGC GCCAGCCGAA GTCAAGACGG TCCCCAATGA CGCCACACAG
ACAAAGGAGA ACGAGAGCAA AGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu20922
Clone ID Related Accession (Same CDS sequence) NM_181351
Accession Version NM_181351.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2577bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neural cell adhesion molecule 1 isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KF511120.1, DA337649.1, BC047244.1, S71824.1, BC014205.2, AK054570.1 and AW131780.1. This sequence is a reference standard in the RefSeqGene project. On Jun 17, 2011 this sequence version replaced gi:117320546. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region compared to variant 5. The resulting protein (isoform 2) is shorter compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047244.1, AK292453.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGG CTTCGTGGAC TCGACCAGAG
AAGCAAGAGA CTCTGGATGG GCACATGGTG GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG
ACCCTGAAGA GCATCCAGTA CACTGATGCC GGAGAGTACA TCTGCACCGC CAGCAACACC
ATCGGCCAGG ACTCCCAGTC CATGTACCTT GAAGTGCAAT ATGCCCCAAA GCTACAGGGC
CCTGTGGCTG TGTACACTTG GGAGGGGAAC CAGGTGAACA TCACCTGCGA GGTATTTGCC
TATCCCAGTG CCACGATCTC ATGGTTTCGG GATGGCCAGC TGCTGCCAAG CTCCAATTAC
AGCAATATCA AGATCTACAA CACCCCCTCT GCCAGCTATC TGGAGGTGAC CCCAGACTCT
GAGAATGATT TTGGGAACTA CAACTGTACT GCAGTGAACC GCATTGGGCA GGAGTCCTTG
GAATTCATCC TTGTTCAAGC AGACACCCCC TCTTCACCAT CCATCGACCA GGTGGAGCCA
TACTCCAGCA CAGCCCAGGT GCAGTTTGAT GAACCAGAGG CCACAGGTGG GGTGCCCATC
CTCAAATACA AAGCTGAGTG GAGAGCAGTT GGTGAAGAAG TATGGCATTC CAAGTGGTAT
GATGCCAAGG AAGCCAGCAT GGAGGGCATC GTCACCATCG TGGGCCTGAA GCCCGAAACA
ACGTACGCCG TAAGGCTGGC GGCGCTCAAT GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC
TCCGAGTTCA AGACGCAGCC AGTCCAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
ATGGGAGAGG ATGGAAACTC TATTAAAGTG AACCTGATCA AGCAGGATGA CGGCGGCTCC
CCCATCAGAC ACTATCTGGT CAGGTACCGA GCGCTCTCCT CCGAGTGGAA ACCAGAGATC
AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTGAAGTCCC TGGACTGGAA TGCTGAGTAT
GAGGTCTACG TGGTGGCTGA GAACCAGCAA GGAAAATCCA AGGCGGCTCA TTTTGTGTTC
AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC AGGCCTGAGC
ACCGGGGCCA TCGTGGGCAT CCTCATCGTC ATCTTCGTCC TGCTCCTGGT GGTTGTGGAC
ATCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGTTCATGT GCATTGCGGT CAACCTGTGT
GGAAAAGCCG GGCCCGGGGC CAAGGGCAAG GACATGGAGG AGGGCAAGGC CGCCTTCTCG
AAAGATGAGT CCAAGGAGCC CATCGTGGAG GTTCGAACGG AGGAGGAGAG GACCCCAAAC
CATGATGGAG GGAAACACAC AGAGCCCAAC GAGACCACGC CACTGACGGA GCCCGAGAAG
GGCCCCGTAG AAGCAAAGCC AGAGTGCCAG GAGACAGAAA CGAAGCCAGC GCCAGCCGAA
GTCAAGACGG TCCCCAATGA CGCCACACAG ACAAAGGAGA ACGAGAGCAA AGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_851996.2
CDS367..2943
Misc Feature(1)424..711(+)
Misc Feature(2)427..702(+)
Misc Feature(3)469..480(+)
Misc Feature(4)757..942(+)
Misc Feature(5)769..936(+)
Misc Feature(6)997..1287(+)
Misc Feature(7)1030..1278(+)
Misc Feature(8)1282..1602(+)
Misc Feature(9)1333..1572(+)
Misc Feature(10)1627..1863(+)
Misc Feature(11)1660..1872(+)
Misc Feature(12)1741..1743(+)
Misc Feature(13)1828..1830(+)
Misc Feature(14)1888..2181(+)
Misc Feature(15)2098..2145(+)
Misc Feature(16)2146..2160(+)
Misc Feature(17)2242..2472(+)
Misc Feature(18)2392..2439(+)
Misc Feature(19)2440..2454(+)
Misc Feature(20)2521..2583(+)
Exon (1)1..418
Gene:NCAM1
Gene Synonym:
Exon (2)419..493
Gene:NCAM1
Gene Synonym:
Exon (3)494..712
Gene:NCAM1
Gene Synonym:
Exon (4)713..856
Gene:NCAM1
Gene Synonym:
Exon (5)857..994
Gene:NCAM1
Gene Synonym:
Exon (6)995..1112
Gene:NCAM1
Gene Synonym:
Exon (7)1113..1282
Gene:NCAM1
Gene Synonym:
Exon (8)1283..1425
Gene:NCAM1
Gene Synonym:
Exon (9)1426..1455
Gene:NCAM1
Gene Synonym:
Exon (10)1456..1606
Gene:NCAM1
Gene Synonym:
Exon (11)1607..1791
Gene:NCAM1
Gene Synonym:
Exon (12)1792..1888
Gene:NCAM1
Gene Synonym:
Exon (13)1889..2059
Gene:NCAM1
Gene Synonym:
Exon (14)2060..2191
Gene:NCAM1
Gene Synonym:
Exon (15)2192..2194
Gene:NCAM1
Gene Synonym:
Exon (16)2195..2319
Gene:NCAM1
Gene Synonym:
Exon (17)2320..2497
Gene:NCAM1
Gene Synonym:
Exon (18)2498..2705
Gene:NCAM1
Gene Synonym:
Exon (19)2706..2822
Gene:NCAM1
Gene Synonym:
Exon (20)2823..5992
Gene:NCAM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_181351
Organism Homo sapiens (human)
Definition Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_181351

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGG CTTCGTGGAC TCGACCAGAG
AAGCAAGAGA CTCTGGATGG GCACATGGTG GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG
ACCCTGAAGA GCATCCAGTA CACTGATGCC GGAGAGTACA TCTGCACCGC CAGCAACACC
ATCGGCCAGG ACTCCCAGTC CATGTACCTT GAAGTGCAAT ATGCCCCAAA GCTACAGGGC
CCTGTGGCTG TGTACACTTG GGAGGGGAAC CAGGTGAACA TCACCTGCGA GGTATTTGCC
TATCCCAGTG CCACGATCTC ATGGTTTCGG GATGGCCAGC TGCTGCCAAG CTCCAATTAC
AGCAATATCA AGATCTACAA CACCCCCTCT GCCAGCTATC TGGAGGTGAC CCCAGACTCT
GAGAATGATT TTGGGAACTA CAACTGTACT GCAGTGAACC GCATTGGGCA GGAGTCCTTG
GAATTCATCC TTGTTCAAGC AGACACCCCC TCTTCACCAT CCATCGACCA GGTGGAGCCA
TACTCCAGCA CAGCCCAGGT GCAGTTTGAT GAACCAGAGG CCACAGGTGG GGTGCCCATC
CTCAAATACA AAGCTGAGTG GAGAGCAGTT GGTGAAGAAG TATGGCATTC CAAGTGGTAT
GATGCCAAGG AAGCCAGCAT GGAGGGCATC GTCACCATCG TGGGCCTGAA GCCCGAAACA
ACGTACGCCG TAAGGCTGGC GGCGCTCAAT GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC
TCCGAGTTCA AGACGCAGCC AGTCCAAGGG GAACCCAGTG CACCTAAGCT CGAAGGGCAG
ATGGGAGAGG ATGGAAACTC TATTAAAGTG AACCTGATCA AGCAGGATGA CGGCGGCTCC
CCCATCAGAC ACTATCTGGT CAGGTACCGA GCGCTCTCCT CCGAGTGGAA ACCAGAGATC
AGGCTCCCGT CTGGCAGTGA CCACGTCATG CTGAAGTCCC TGGACTGGAA TGCTGAGTAT
GAGGTCTACG TGGTGGCTGA GAACCAGCAA GGAAAATCCA AGGCGGCTCA TTTTGTGTTC
AGGACCTCGG CCCAGCCCAC AGCCATCCCA GCCAACGGCA GCCCCACCTC AGGCCTGAGC
ACCGGGGCCA TCGTGGGCAT CCTCATCGTC ATCTTCGTCC TGCTCCTGGT GGTTGTGGAC
ATCACCTGCT ACTTCCTGAA CAAGTGTGGC CTGTTCATGT GCATTGCGGT CAACCTGTGT
GGAAAAGCCG GGCCCGGGGC CAAGGGCAAG GACATGGAGG AGGGCAAGGC CGCCTTCTCG
AAAGATGAGT CCAAGGAGCC CATCGTGGAG GTTCGAACGG AGGAGGAGAG GACCCCAAAC
CATGATGGAG GGAAACACAC AGAGCCCAAC GAGACCACGC CACTGACGGA GCCCGAGAAG
GGCCCCGTAG AAGCAAAGCC AGAGTGCCAG GAGACAGAAA CGAAGCCAGC GCCAGCCGAA
GTCAAGACGG TCCCCAATGA CGCCACACAG ACAAAGGAGA ACGAGAGCAA AGCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00364
Clone ID Related Accession (Same CDS sequence) NM_001076682
Accession Version NM_001076682.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neural cell adhesion molecule 1 isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KF511120.1, DA337649.1, BC047244.1, S71824.1, X16841.1, AB209443.1 and BC019845.1. On Jun 17, 2011 this sequence version replaced gi:117320541. Transcript Variant: This variant (3) has multiple differences in the coding region and uses an alternate splice site in the 3' coding region compared to variant 5. The resulting protein (isoform 3) is shorter and has a distinct C-terminus compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X16841.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGA CTCTGGATGG GCACATGGTG
GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG ACCCTGAAGA GCATCCAGTA CACTGATGCC
GGAGAGTACA TCTGCACCGC CAGCAACACC ATCGGCCAGG ACTCCCAGTC CATGTACCTT
GAAGTGCAAT ATGCCCCAAA GCTACAGGGC CCTGTGGCTG TGTACACTTG GGAGGGGAAC
CAGGTGAACA TCACCTGCGA GGTATTTGCC TATCCCAGTG CCACGATCTC ATGGTTTCGG
GATGGCCAGC TGCTGCCAAG CTCCAATTAC AGCAATATCA AGATCTACAA CACCCCCTCT
GCCAGCTATC TGGAGGTGAC CCCAGACTCT GAGAATGATT TTGGGAACTA CAACTGTACT
GCAGTGAACC GCATTGGGCA GGAGTCCTTG GAATTCATCC TTGTTCAAGC AGACACCCCC
TCTTCACCAT CCATCGACCA GGTGGAGCCA TACTCCAGCA CAGCCCAGGT GCAGTTTGAT
GAACCAGAGG CCACAGGTGG GGTGCCCATC CTCAAATACA AAGCTGAGTG GAGAGCAGTT
GGTGAAGAAG TATGGCATTC CAAGTGGTAT GATGCCAAGG AAGCCAGCAT GGAGGGCATC
GTCACCATCG TGGGCCTGAA GCCCGAAACA ACGTACGCCG TAAGGCTGGC GGCGCTCAAT
GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC TCCGAGTTCA AGACGCAGCC AGTCCATAGC
CCTCCTCCAC CGGCATCTGC TAGCTCGTCT ACCCCTGTTC CATTGTCTCC ACCAGATACA
ACTTGGCCTC TTCCTGCCCT TGCAACCACA GAACCAGCTA AAGGGGAACC CAGTGCACCT
AAGCTCGAAG GGCAGATGGG AGAGGATGGA AACTCTATTA AAGTGAACCT GATCAAGCAG
GATGACGGCG GCTCCCCCAT CAGACACTAT CTGGTCAGGT ACCGAGCGCT CTCCTCCGAG
TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTGAA GTCCCTGGAC
TGGAATGCTG AGTATGAGGT CTACGTGGTG GCTGAGAACC AGCAAGGAAA ATCCAAGGCG
GCTCATTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCAAC CTTGGGAGGC
AATTCTGCAT CCTACACCTT TGTCTCATTG CTTTTCTCTG CAGTGACTCT TCTTTTGCTC
TGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001070150.1
CDS367..2652
Misc Feature(1)424..711(+)
Misc Feature(2)427..702(+)
Misc Feature(3)469..480(+)
Misc Feature(4)757..942(+)
Misc Feature(5)769..936(+)
Misc Feature(6)997..1287(+)
Misc Feature(7)1030..1278(+)
Misc Feature(8)1282..1572(+)
Misc Feature(9)1333..1542(+)
Misc Feature(10)1618..1824(+)
Misc Feature(11)1630..1842(+)
Misc Feature(12)1858..2151(+)
Misc Feature(13)2068..2115(+)
Misc Feature(14)2116..2130(+)
Misc Feature(15)2317..2547(+)
Misc Feature(16)2467..2514(+)
Misc Feature(17)2515..2529(+)
Exon (1)1..418
Gene:NCAM1
Gene Synonym:
Exon (2)419..493
Gene:NCAM1
Gene Synonym:
Exon (3)494..712
Gene:NCAM1
Gene Synonym:
Exon (4)713..856
Gene:NCAM1
Gene Synonym:
Exon (5)857..994
Gene:NCAM1
Gene Synonym:
Exon (6)995..1112
Gene:NCAM1
Gene Synonym:
Exon (7)1113..1282
Gene:NCAM1
Gene Synonym:
Exon (8)1283..1425
Gene:NCAM1
Gene Synonym:
Exon (9)1426..1576
Gene:NCAM1
Gene Synonym:
Exon (10)1577..1761
Gene:NCAM1
Gene Synonym:
Exon (11)1762..1858
Gene:NCAM1
Gene Synonym:
Exon (12)1859..2029
Gene:NCAM1
Gene Synonym:
Exon (13)2030..2161
Gene:NCAM1
Gene Synonym:
Exon (14)2162..2176
Gene:NCAM1
Gene Synonym:
Exon (15)2177..2224
Gene:NCAM1
Gene Synonym:
Exon (16)2225..2266
Gene:NCAM1
Gene Synonym:
Exon (17)2267..2269
Gene:NCAM1
Gene Synonym:
Exon (18)2270..2394
Gene:NCAM1
Gene Synonym:
Exon (19)2395..2572
Gene:NCAM1
Gene Synonym:
Exon (20)2573..4924
Gene:NCAM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001076682
Organism Homo sapiens (human)
Definition Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001076682

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGA CTCTGGATGG GCACATGGTG
GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG ACCCTGAAGA GCATCCAGTA CACTGATGCC
GGAGAGTACA TCTGCACCGC CAGCAACACC ATCGGCCAGG ACTCCCAGTC CATGTACCTT
GAAGTGCAAT ATGCCCCAAA GCTACAGGGC CCTGTGGCTG TGTACACTTG GGAGGGGAAC
CAGGTGAACA TCACCTGCGA GGTATTTGCC TATCCCAGTG CCACGATCTC ATGGTTTCGG
GATGGCCAGC TGCTGCCAAG CTCCAATTAC AGCAATATCA AGATCTACAA CACCCCCTCT
GCCAGCTATC TGGAGGTGAC CCCAGACTCT GAGAATGATT TTGGGAACTA CAACTGTACT
GCAGTGAACC GCATTGGGCA GGAGTCCTTG GAATTCATCC TTGTTCAAGC AGACACCCCC
TCTTCACCAT CCATCGACCA GGTGGAGCCA TACTCCAGCA CAGCCCAGGT GCAGTTTGAT
GAACCAGAGG CCACAGGTGG GGTGCCCATC CTCAAATACA AAGCTGAGTG GAGAGCAGTT
GGTGAAGAAG TATGGCATTC CAAGTGGTAT GATGCCAAGG AAGCCAGCAT GGAGGGCATC
GTCACCATCG TGGGCCTGAA GCCCGAAACA ACGTACGCCG TAAGGCTGGC GGCGCTCAAT
GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC TCCGAGTTCA AGACGCAGCC AGTCCATAGC
CCTCCTCCAC CGGCATCTGC TAGCTCGTCT ACCCCTGTTC CATTGTCTCC ACCAGATACA
ACTTGGCCTC TTCCTGCCCT TGCAACCACA GAACCAGCTA AAGGGGAACC CAGTGCACCT
AAGCTCGAAG GGCAGATGGG AGAGGATGGA AACTCTATTA AAGTGAACCT GATCAAGCAG
GATGACGGCG GCTCCCCCAT CAGACACTAT CTGGTCAGGT ACCGAGCGCT CTCCTCCGAG
TGGAAACCAG AGATCAGGCT CCCGTCTGGC AGTGACCACG TCATGCTGAA GTCCCTGGAC
TGGAATGCTG AGTATGAGGT CTACGTGGTG GCTGAGAACC AGCAAGGAAA ATCCAAGGCG
GCTCATTTTG TGTTCAGGAC CTCGGCCCAG CCCACAGCCA TCCCAGCAAC CTTGGGAGGC
AATTCTGCAT CCTACACCTT TGTCTCATTG CTTTTCTCTG CAGTGACTCT TCTTTTGCTC
TGTTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00345
Clone ID Related Accession (Same CDS sequence) NM_001242607
Accession Version NM_001242607.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2655bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neural cell adhesion molecule 1 isoform 5 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KF511120.1, AK314589.1, AP002853.4 and AP000880.4. Transcript Variant: This variant (5) represents the longest transcript and encodes the longest protein (isoform 5). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314589.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGG CTTCGTGGAC TCGACCAGAG
AAGCAAGAGG TACATGCACC ATGGAACTGG CAAGTGGGCA GACAGAAAGG ACAGGCTGGC
AGTGCAGGTT TCCCAGGATC TCATGAGACT CTGGATGGGC ACATGGTGGT GCGTAGCCAT
GCCCGTGTGT CGTCGCTGAC CCTGAAGAGC ATCCAGTACA CTGATGCCGG AGAGTACATC
TGCACCGCCA GCAACACCAT CGGCCAGGAC TCCCAGTCCA TGTACCTTGA AGTGCAATAT
GCCCCAAAGC TACAGGGCCC TGTGGCTGTG TACACTTGGG AGGGGAACCA GGTGAACATC
ACCTGCGAGG TATTTGCCTA TCCCAGTGCC ACGATCTCAT GGTTTCGGGA TGGCCAGCTG
CTGCCAAGCT CCAATTACAG CAATATCAAG ATCTACAACA CCCCCTCTGC CAGCTATCTG
GAGGTGACCC CAGACTCTGA GAATGATTTT GGGAACTACA ACTGTACTGC AGTGAACCGC
ATTGGGCAGG AGTCCTTGGA ATTCATCCTT GTTCAAGCAG ACACCCCCTC TTCACCATCC
ATCGACCAGG TGGAGCCATA CTCCAGCACA GCCCAGGTGC AGTTTGATGA ACCAGAGGCC
ACAGGTGGGG TGCCCATCCT CAAATACAAA GCTGAGTGGA GAGCAGTTGG TGAAGAAGTA
TGGCATTCCA AGTGGTATGA TGCCAAGGAA GCCAGCATGG AGGGCATCGT CACCATCGTG
GGCCTGAAGC CCGAAACAAC GTACGCCGTA AGGCTGGCGG CGCTCAATGG CAAAGGGCTG
GGTGAGATCA GCGCGGCCTC CGAGTTCAAG ACGCAGCCAG TCCAAGGGGA ACCCAGTGCA
CCTAAGCTCG AAGGGCAGAT GGGAGAGGAT GGAAACTCTA TTAAAGTGAA CCTGATCAAG
CAGGATGACG GCGGCTCCCC CATCAGACAC TATCTGGTCA GGTACCGAGC GCTCTCCTCC
GAGTGGAAAC CAGAGATCAG GCTCCCGTCT GGCAGTGACC ACGTCATGCT GAAGTCCCTG
GACTGGAATG CTGAGTATGA GGTCTACGTG GTGGCTGAGA ACCAGCAAGG AAAATCCAAG
GCGGCTCATT TTGTGTTCAG GACCTCGGCC CAGCCCACAG CCATCCCAGC CAACGGCAGC
CCCACCTCAG GCCTGAGCAC CGGGGCCATC GTGGGCATCC TCATCGTCAT CTTCGTCCTG
CTCCTGGTGG TTGTGGACAT CACCTGCTAC TTCCTGAACA AGTGTGGCCT GTTCATGTGC
ATTGCGGTCA ACCTGTGTGG AAAAGCCGGG CCCGGGGCCA AGGGCAAGGA CATGGAGGAG
GGCAAGGCCG CCTTCTCGAA AGATGAGTCC AAGGAGCCCA TCGTGGAGGT TCGAACGGAG
GAGGAGAGGA CCCCAAACCA TGATGGAGGG AAACACACAG AGCCCAACGA GACCACGCCA
CTGACGGAGC CCGAGAAGGG CCCCGTAGAA GCAAAGCCAG AGTGCCAGGA GACAGAAACG
AAGCCAGCGC CAGCCGAAGT CAAGACGGTC CCCAATGACG CCACACAGAC AAAGGAGAAC
GAGAGCAAAG CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001229536.1
CDS367..3021
Misc Feature(1)424..711(+)
Misc Feature(2)427..702(+)
Misc Feature(3)469..480(+)
Misc Feature(4)757..942(+)
Misc Feature(5)769..936(+)
Misc Feature(6)997..1287(+)
Misc Feature(7)1030..1278(+)
Misc Feature(8)1282..1680(+)
Misc Feature(9)1333..1650(+)
Misc Feature(10)1705..1941(+)
Misc Feature(11)1738..1950(+)
Misc Feature(12)1966..2259(+)
Misc Feature(13)2176..2223(+)
Misc Feature(14)2224..2238(+)
Misc Feature(15)2320..2550(+)
Misc Feature(16)2470..2517(+)
Misc Feature(17)2518..2532(+)
Exon (1)1..418
Gene:NCAM1
Gene Synonym:
Exon (2)419..493
Gene:NCAM1
Gene Synonym:
Exon (3)494..712
Gene:NCAM1
Gene Synonym:
Exon (4)713..856
Gene:NCAM1
Gene Synonym:
Exon (5)857..994
Gene:NCAM1
Gene Synonym:
Exon (6)995..1112
Gene:NCAM1
Gene Synonym:
Exon (7)1113..1282
Gene:NCAM1
Gene Synonym:
Exon (8)1283..1425
Gene:NCAM1
Gene Synonym:
Exon (9)1426..1455
Gene:NCAM1
Gene Synonym:
Exon (10)1456..1533
Gene:NCAM1
Gene Synonym:
Exon (11)1534..1684
Gene:NCAM1
Gene Synonym:
Exon (12)1685..1869
Gene:NCAM1
Gene Synonym:
Exon (13)1870..1966
Gene:NCAM1
Gene Synonym:
Exon (14)1967..2137
Gene:NCAM1
Gene Synonym:
Exon (15)2138..2269
Gene:NCAM1
Gene Synonym:
Exon (16)2270..2272
Gene:NCAM1
Gene Synonym:
Exon (17)2273..2397
Gene:NCAM1
Gene Synonym:
Exon (18)2398..2575
Gene:NCAM1
Gene Synonym:
Exon (19)2576..2783
Gene:NCAM1
Gene Synonym:
Exon (20)2784..2900
Gene:NCAM1
Gene Synonym:
Exon (21)2901..6070
Gene:NCAM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001242607
Organism Homo sapiens (human)
Definition Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001242607

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGG CTTCGTGGAC TCGACCAGAG
AAGCAAGAGG TACATGCACC ATGGAACTGG CAAGTGGGCA GACAGAAAGG ACAGGCTGGC
AGTGCAGGTT TCCCAGGATC TCATGAGACT CTGGATGGGC ACATGGTGGT GCGTAGCCAT
GCCCGTGTGT CGTCGCTGAC CCTGAAGAGC ATCCAGTACA CTGATGCCGG AGAGTACATC
TGCACCGCCA GCAACACCAT CGGCCAGGAC TCCCAGTCCA TGTACCTTGA AGTGCAATAT
GCCCCAAAGC TACAGGGCCC TGTGGCTGTG TACACTTGGG AGGGGAACCA GGTGAACATC
ACCTGCGAGG TATTTGCCTA TCCCAGTGCC ACGATCTCAT GGTTTCGGGA TGGCCAGCTG
CTGCCAAGCT CCAATTACAG CAATATCAAG ATCTACAACA CCCCCTCTGC CAGCTATCTG
GAGGTGACCC CAGACTCTGA GAATGATTTT GGGAACTACA ACTGTACTGC AGTGAACCGC
ATTGGGCAGG AGTCCTTGGA ATTCATCCTT GTTCAAGCAG ACACCCCCTC TTCACCATCC
ATCGACCAGG TGGAGCCATA CTCCAGCACA GCCCAGGTGC AGTTTGATGA ACCAGAGGCC
ACAGGTGGGG TGCCCATCCT CAAATACAAA GCTGAGTGGA GAGCAGTTGG TGAAGAAGTA
TGGCATTCCA AGTGGTATGA TGCCAAGGAA GCCAGCATGG AGGGCATCGT CACCATCGTG
GGCCTGAAGC CCGAAACAAC GTACGCCGTA AGGCTGGCGG CGCTCAATGG CAAAGGGCTG
GGTGAGATCA GCGCGGCCTC CGAGTTCAAG ACGCAGCCAG TCCAAGGGGA ACCCAGTGCA
CCTAAGCTCG AAGGGCAGAT GGGAGAGGAT GGAAACTCTA TTAAAGTGAA CCTGATCAAG
CAGGATGACG GCGGCTCCCC CATCAGACAC TATCTGGTCA GGTACCGAGC GCTCTCCTCC
GAGTGGAAAC CAGAGATCAG GCTCCCGTCT GGCAGTGACC ACGTCATGCT GAAGTCCCTG
GACTGGAATG CTGAGTATGA GGTCTACGTG GTGGCTGAGA ACCAGCAAGG AAAATCCAAG
GCGGCTCATT TTGTGTTCAG GACCTCGGCC CAGCCCACAG CCATCCCAGC CAACGGCAGC
CCCACCTCAG GCCTGAGCAC CGGGGCCATC GTGGGCATCC TCATCGTCAT CTTCGTCCTG
CTCCTGGTGG TTGTGGACAT CACCTGCTAC TTCCTGAACA AGTGTGGCCT GTTCATGTGC
ATTGCGGTCA ACCTGTGTGG AAAAGCCGGG CCCGGGGCCA AGGGCAAGGA CATGGAGGAG
GGCAAGGCCG CCTTCTCGAA AGATGAGTCC AAGGAGCCCA TCGTGGAGGT TCGAACGGAG
GAGGAGAGGA CCCCAAACCA TGATGGAGGG AAACACACAG AGCCCAACGA GACCACGCCA
CTGACGGAGC CCGAGAAGGG CCCCGTAGAA GCAAAGCCAG AGTGCCAGGA GACAGAAACG
AAGCCAGCGC CAGCCGAAGT CAAGACGGTC CCCAATGACG CCACACAGAC AAAGGAGAAC
GAGAGCAAAG CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu00771
Clone ID Related Accession (Same CDS sequence) NM_001242608
Accession Version NM_001242608.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product neural cell adhesion molecule 1 isoform 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from KF511120.1, AB209443.1 and AA029449.1. Transcript Variant: This variant (4) lacks 2 alternate in-frame exons and uses an alternate splice site in the 3' coding region compared to variant 5. The resulting protein (isoform 4) is shorter and has a distinct C-terminus compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB209443.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGA CTCTGGATGG GCACATGGTG
GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG ACCCTGAAGA GCATCCAGTA CACTGATGCC
GGAGAGTACA TCTGCACCGC CAGCAACACC ATCGGCCAGG ACTCCCAGTC CATGTACCTT
GAAGTGCAAT ATGCCCCAAA GCTACAGGGC CCTGTGGCTG TGTACACTTG GGAGGGGAAC
CAGGTGAACA TCACCTGCGA GGTATTTGCC TATCCCAGTG CCACGATCTC ATGGTTTCGG
GATGGCCAGC TGCTGCCAAG CTCCAATTAC AGCAATATCA AGATCTACAA CACCCCCTCT
GCCAGCTATC TGGAGGTGAC CCCAGACTCT GAGAATGATT TTGGGAACTA CAACTGTACT
GCAGTGAACC GCATTGGGCA GGAGTCCTTG GAATTCATCC TTGTTCAAGC AGACACCCCC
TCTTCACCAT CCATCGACCA GGTGGAGCCA TACTCCAGCA CAGCCCAGGT GCAGTTTGAT
GAACCAGAGG CCACAGGTGG GGTGCCCATC CTCAAATACA AAGCTGAGTG GAGAGCAGTT
GGTGAAGAAG TATGGCATTC CAAGTGGTAT GATGCCAAGG AAGCCAGCAT GGAGGGCATC
GTCACCATCG TGGGCCTGAA GCCCGAAACA ACGTACGCCG TAAGGCTGGC GGCGCTCAAT
GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC TCCGAGTTCA AGACGCAGCC AGTCCAAGGG
GAACCCAGTG CACCTAAGCT CGAAGGGCAG ATGGGAGAGG ATGGAAACTC TATTAAAGTG
AACCTGATCA AGCAGGATGA CGGCGGCTCC CCCATCAGAC ACTATCTGGT CAGGTACCGA
GCGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
CTGAAGTCCC TGGACTGGAA TGCTGAGTAT GAGGTCTACG TGGTGGCTGA GAACCAGCAA
GGAAAATCCA AGGCGGCTCA TTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
GCAACCTTGG GAGGCAATTC TGCATCCTAC ACCTTTGTCT CATTGCTTTT CTCTGCAGTG
ACTCTTCTTT TGCTCTGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001229537.1
CDS367..2547
Misc Feature(1)424..711(+)
Misc Feature(2)427..702(+)
Misc Feature(3)469..480(+)
Misc Feature(4)757..942(+)
Misc Feature(5)769..936(+)
Misc Feature(6)997..1287(+)
Misc Feature(7)1030..1278(+)
Misc Feature(8)1282..1572(+)
Misc Feature(9)1333..1542(+)
Misc Feature(10)1618..1824(+)
Misc Feature(11)1630..1842(+)
Misc Feature(12)1858..2151(+)
Misc Feature(13)2068..2115(+)
Misc Feature(14)2116..2130(+)
Misc Feature(15)2212..2442(+)
Misc Feature(16)2362..2409(+)
Misc Feature(17)2410..2424(+)
Exon (1)1..418
Gene:NCAM1
Gene Synonym:
Exon (2)419..493
Gene:NCAM1
Gene Synonym:
Exon (3)494..712
Gene:NCAM1
Gene Synonym:
Exon (4)713..856
Gene:NCAM1
Gene Synonym:
Exon (5)857..994
Gene:NCAM1
Gene Synonym:
Exon (6)995..1112
Gene:NCAM1
Gene Synonym:
Exon (7)1113..1282
Gene:NCAM1
Gene Synonym:
Exon (8)1283..1425
Gene:NCAM1
Gene Synonym:
Exon (9)1426..1576
Gene:NCAM1
Gene Synonym:
Exon (10)1577..1761
Gene:NCAM1
Gene Synonym:
Exon (11)1762..1858
Gene:NCAM1
Gene Synonym:
Exon (12)1859..2029
Gene:NCAM1
Gene Synonym:
Exon (13)2030..2161
Gene:NCAM1
Gene Synonym:
Exon (14)2162..2164
Gene:NCAM1
Gene Synonym:
Exon (15)2165..2289
Gene:NCAM1
Gene Synonym:
Exon (16)2290..2467
Gene:NCAM1
Gene Synonym:
Exon (17)2468..4819
Gene:NCAM1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001242608
Organism Homo sapiens (human)
Definition Homo sapiens neural cell adhesion molecule 1 (NCAM1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001242608

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCTGCAAA CTAAGGATCT CATCTGGACT TTGTTTTTCC TGGGAACTGC AGTTTCTCTG 
CAGGTGGATA TTGTTCCCAG CCAGGGGGAG ATCAGCGTTG GAGAGTCCAA ATTCTTCTTA
TGCCAAGTGG CAGGAGATGC CAAAGATAAA GACATCTCCT GGTTCTCCCC CAATGGAGAA
AAGCTCACCC CAAACCAGCA GCGGATCTCA GTGGTGTGGA ATGATGATTC CTCCTCCACC
CTCACCATCT ATAACGCCAA CATCGACGAC GCCGGCATTT ACAAGTGTGT GGTTACAGGC
GAGGATGGCA GTGAGTCAGA GGCCACCGTC AACGTGAAGA TCTTTCAGAA GCTCATGTTC
AAGAATGCGC CAACCCCACA GGAGTTCCGG GAGGGGGAAG ATGCCGTGAT TGTGTGTGAT
GTGGTCAGCT CCCTCCCACC AACCATCATC TGGAAACACA AAGGCCGAGA TGTCATCCTG
AAAAAAGATG TCCGATTCAT AGTCCTGTCC AACAACTACC TGCAGATCCG GGGCATCAAG
AAAACAGATG AGGGCACTTA TCGCTGTGAG GGCAGAATCC TGGCACGGGG GGAGATCAAC
TTCAAGGACA TTCAGGTCAT TGTGAATGTG CCACCTACCA TCCAGGCCAG GCAGAATATT
GTGAATGCCA CCGCCAACCT CGGCCAGTCC GTCACCCTGG TGTGCGATGC CGAAGGCTTC
CCAGAGCCCA CCATGAGCTG GACAAAGGAT GGGGAACAGA TAGAGCAAGA GGAAGACGAT
GAGAAGTACA TCTTCAGCGA CGATAGTTCC CAGCTGACCA TCAAAAAGGT GGATAAGAAC
GACGAGGCTG AGTACATCTG CATTGCTGAG AACAAGGCTG GCGAGCAGGA TGCGACCATC
CACCTCAAAG TCTTTGCAAA ACCCAAAATC ACATATGTAG AGAACCAGAC TGCCATGGAA
TTAGAGGAGC AGGTCACTCT TACCTGTGAA GCCTCCGGAG ACCCCATTCC CTCCATCACC
TGGAGGACTT CTACCCGGAA CATCAGCAGC GAAGAAAAGA CTCTGGATGG GCACATGGTG
GTGCGTAGCC ATGCCCGTGT GTCGTCGCTG ACCCTGAAGA GCATCCAGTA CACTGATGCC
GGAGAGTACA TCTGCACCGC CAGCAACACC ATCGGCCAGG ACTCCCAGTC CATGTACCTT
GAAGTGCAAT ATGCCCCAAA GCTACAGGGC CCTGTGGCTG TGTACACTTG GGAGGGGAAC
CAGGTGAACA TCACCTGCGA GGTATTTGCC TATCCCAGTG CCACGATCTC ATGGTTTCGG
GATGGCCAGC TGCTGCCAAG CTCCAATTAC AGCAATATCA AGATCTACAA CACCCCCTCT
GCCAGCTATC TGGAGGTGAC CCCAGACTCT GAGAATGATT TTGGGAACTA CAACTGTACT
GCAGTGAACC GCATTGGGCA GGAGTCCTTG GAATTCATCC TTGTTCAAGC AGACACCCCC
TCTTCACCAT CCATCGACCA GGTGGAGCCA TACTCCAGCA CAGCCCAGGT GCAGTTTGAT
GAACCAGAGG CCACAGGTGG GGTGCCCATC CTCAAATACA AAGCTGAGTG GAGAGCAGTT
GGTGAAGAAG TATGGCATTC CAAGTGGTAT GATGCCAAGG AAGCCAGCAT GGAGGGCATC
GTCACCATCG TGGGCCTGAA GCCCGAAACA ACGTACGCCG TAAGGCTGGC GGCGCTCAAT
GGCAAAGGGC TGGGTGAGAT CAGCGCGGCC TCCGAGTTCA AGACGCAGCC AGTCCAAGGG
GAACCCAGTG CACCTAAGCT CGAAGGGCAG ATGGGAGAGG ATGGAAACTC TATTAAAGTG
AACCTGATCA AGCAGGATGA CGGCGGCTCC CCCATCAGAC ACTATCTGGT CAGGTACCGA
GCGCTCTCCT CCGAGTGGAA ACCAGAGATC AGGCTCCCGT CTGGCAGTGA CCACGTCATG
CTGAAGTCCC TGGACTGGAA TGCTGAGTAT GAGGTCTACG TGGTGGCTGA GAACCAGCAA
GGAAAATCCA AGGCGGCTCA TTTTGTGTTC AGGACCTCGG CCCAGCCCAC AGCCATCCCA
GCAACCTTGG GAGGCAATTC TGCATCCTAC ACCTTTGTCT CATTGCTTTT CTCTGCAGTG
ACTCTTCTTT TGCTCTGTTA G

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Cytoplasmic domain of NCAM140 interacts with ubiquitin-fold modifier-conjugating enzyme-1 (Ufc1)
Exp. Cell Res. 324 (2), 192-199 (2014)
Homrich M, Wobst H, Laurini C, Sabrowski J, Schmitz B and Diestel S.


book

Immune status of Fanconi anemia patients: decrease in T CD8 and CD56dim CD16+ NK lymphocytes
Ann. Hematol. 93 (5), 761-767 (2014)
Justo GA, Bitencourt MA, Pasquini R, Castelo-Branco MT, Almeida-Oliveira A, Diamond HR and Rumjanek VM.


book

Myocardial expression level of neural cell adhesion molecule correlates with reduced left ventricular function in human cardiomyopathy
Circ Heart Fail 7 (2), 351-358 (2014)
Nagao K, Sowa N, Inoue K, Tokunaga M, Fukuchi K, Uchiyama K, Ito H, Hayashi F, Makita T, Inada T, Tanaka M, Kimura T and Ono K.


book

Expression of CD56 is an unfavorable prognostic factor for acute promyelocytic leukemia with higher initial white blood cell counts
Cancer Sci. 105 (1), 97-104 (2014)
Ono T, Takeshita A, Kishimoto Y, Kiyoi H, Okada M, Yamauchi T, Emi N, Horikawa K, Matsuda M, Shinagawa K, Monma F, Ohtake S, Nakaseko C, Takahashi M, Kimura Y, Iwanaga M, Asou N and Naoe T.


book

Genome wide association study (GWAS) of Chagas cardiomyopathy in Trypanosoma cruzi seropositive subjects
PLoS ONE 8 (11), E79629 (2013)
Deng X, Sabino EC, Cunha-Neto E, Ribeiro AL, Ianni B, Mady C, Busch MP and Seielstad M.


book

A discordance between the human muscle NCAM sequence and those seen in other NCAM cDNA clones
Biochim. Biophys. Acta 1130 (1), 95-96 (1992)
Rossell RJ, Malik A, Helfrich W, Kemshead JT and Patel K.


book

Splicing of the VASE exon of neural cell adhesion molecule (NCAM) in human small-cell lung carcinoma (SCLC)
Int. J. Cancer 50 (1), 118-123 (1992)
van Duijnhoven HL, Helfrich W, de Leij L, Roebroek AJ, van de Ven WJ, Healey K, Culverwell A, Rossell RJ, Kemshead JT and Patel K.


book

Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56)
J. Immunol. 146 (12), 4421-4426 (1991)
Lanier LL, Chang C, Azuma M, Ruitenberg JJ, Hemperly JJ and Phillips JH.


book

Precise in situ localization of NCAM, ETS1, and D11S29 on human meiotic chromosomes
Cytogenet. Cell Genet. 52 (1-2), 7-10 (1989)
Bello MJ, Salagnon N, Rey JA, Guichaoua MR, Berge-Lefranc JL, Jordan BR and Luciani JM.


book

Human muscle neural cell adhesion molecule (N-CAM): identification of a muscle-specific sequence in the extracellular domain
Cell 50 (7), 1119-1130 (1987)
Dickson,G., Gower,H.J., Barton,C.H., Prentice,H.M., Elsom,V.L., Moore,S.E., Cox,R.D., Quinn,C., Putt,W. and Walsh,F.S.


 
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