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NCF4 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol NCF4
Entrez Gene ID 4689
Full Name neutrophil cytosolic factor 4
Synonyms CGD3,NCF,P40PHOX,SH3PXD4
General protein information
Preferred Names

neutrophil cytosolic factor 4

Names

neutrophil cytosol factor 4
NCF-4
SH3 and PX domain-containing protein 4
neutrophil NADPH oxidase factor 4
neutrophil cytosolic factor 4, 40kDa
p40-phox

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

22

22q12.3

Summary The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008].
Disorder MIM:

601488


mRNA and Protein(s)

mRNA Protein Name
NM_000631.4 NP_000622.2 neutrophil cytosol factor 4 isoform 1
NM_013416.3 NP_038202.2 neutrophil cytosol factor 4 isoform 2
XM_017028808.1 XP_016884297.1 neutrophil cytosol factor 4 isoform X1



Gallus gallus (chicken) NCF4 NP_001034361.1
Xenopus tropicalis (tropical clawed frog) ncf4 NP_001025692.1
Mus musculus (house mouse) Ncf4 NP_032703.2
Homo sapiens (human) NCF4 NP_000622.2
Rattus norvegicus (Norway rat) Ncf4 NP_001120776.1
Danio rerio (zebrafish) ncf4 NP_991274.2
Pan troglodytes (chimpanzee) NCF4 XP_001159332.2
Canis lupus familiaris (dog) NCF4 XP_538398.2
Bos taurus (cattle) NCF4 NP_001039448.1


Related articles in PubMed

Association between NCF4 rs4821544T/C polymorphism and inflammatory bowel disease risk in Caucasian: a meta-analysis.
Wu PB, Dai JF, Wang Q, Zhang G, Tan SY, Li M, Ye HL
Inflammation research : official journal of the European Histamine Research Society ... [et al.]64(10)825-31(2015 Oct)

Activation of NADPH oxidase subunit NCF4 induces ROS-mediated EMT signaling in HeLa cells.
Kim YM, Cho M
Cellular signalling26(4)784-96(2014 Apr)

Germline variation in NCF4, an innate immunity gene, is associated with an increased risk of colorectal cancer.
Ryan BM, Zanetti KA, Robles AI, Schetter AJ, Goodman J, Hayes RB, Huang WY, Gunter MJ, Yeager M, Burdette L, Berndt SI, Harris CC
International journal of cancer134(6)1399-407(2014 Mar)

Association of anthracycline-related cardiac histological lesions with NADPH oxidase functional polymorphisms.
Cascales A, Pastor-Quirante F, Sánchez-Vega B, Luengo-Gil G, Corral J, Ortuño-Pacheco G, Vicente V, de la Peña FA
The oncologist18(4)446-53(2013)

Cutting edge: NADPH oxidase modulates MHC class II antigen presentation by B cells.
Crotzer VL, Matute JD, Arias AA, Zhao H, Quilliam LA, Dinauer MC, Blum JS
Journal of immunology (Baltimore, Md. : 1950)189(8)3800-4(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phosphoinositol 3-phosphate regulates reactive oxygen species production by maintaining p40phox and p67phox at the phagosomal membrane.
Title: Phosphoinositol 3-phosphate acts as a timer for reactive oxygen species production in the phagosome.

NCF4 polymorphism was associated with Crohn's disease, but not ulcerative colitis in Caucasian--{REVIEW}
Title: Association between NCF4 rs4821544T/C polymorphism and inflammatory bowel disease risk in Caucasian: a meta-analysis.

NCF4 may induce ex- pression of NADPH oxidase enzymes, such as p67phox, p47phox, p22phox and NOX2, which lead to increased ROS levels.
Title: Activation of NADPH oxidase subunit NCF4 induces ROS-mediated EMT signaling in HeLa cells.

Germline variation in NCF4, an innate immunity gene, is associated with an increased risk of colorectal cancer.
Title: Germline variation in NCF4, an innate immunity gene, is associated with an increased risk of colorectal cancer.

The contribution of the functionally relevant NADPH polymorphisms rs1883112 and rs4673 to anthracycline-related heart lesions provides a plausible explanation for their modulation of cardiotoxicity.
Title: Association of anthracycline-related cardiac histological lesions with NADPH oxidase functional polymorphisms.

The following NCF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu16465 NM_000631.4
Latest version!
Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu18835 NM_013416.3
Latest version!
Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $265.30
$379.00
OHu93501 XM_017028808.1
Latest version!
Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu16465
Clone ID Related Accession (Same CDS sequence) NM_000631.4
Accession Version NM_000631.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1020bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-02
Organism Homo sapiens(Human)
Product neutrophil cytosol factor 4 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002798.1, BX355636.2 and BU076892.1. On Dec 20, 2007 this sequence version replaced NM_000631.3. Transcript Variant: This variant (1) lacks an internal coding segment compared to variant 2, which leads to a translation frameshift. The resulting isoform (1) has a distinct and shorter C-terminus, as compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.191850.1, SRR1163657.573546.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

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361
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481
541
601
661
721
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841
901
961
ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGG ACATCGCGGT GGAGGAAGAT
CTCAGCAGCA CTCCCCTATT GAAAGACCTG CTGGAGCTCA CAAGGCGGGA GTTCCAGAGA
GAGGACATAG CTCTGAATTA CCGGGACGCT GAGGGGGATC TGGTTCGGCT GCTGTCGGAT
GAGGACGTAG CGCTCATGGT GCGGCAGGCT CGTGGCCTCC CCTCCCAGAA GCGCCTCTTC
CCCTGGAAGC TGCACATCAC GCAGAAGGAC AACTACAGGG TCTACAACAC GATGCCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000622.2
CDS185..1204
Misc Feature(1)122..124
Misc Feature(2)239..607
Misc Feature(3)order(281..283,287..292,296..298,362..364,596..598,605..607)
Misc Feature(4)order(356..364,458..463,497..499)
Misc Feature(5)356..364
Misc Feature(6)458..466
Misc Feature(7)644..646
Misc Feature(8)644..646
Misc Feature(9)704..865
Misc Feature(10)order(719..724,734..736,746..748,791..796,800..805,842..844,851..853)
Misc Feature(11)order(782..784,821..826,830..832)
Misc Feature(12)896..1171
Misc Feature(13)order(953..955,977..979,1001..1003,1142..1144,1148..1150)
Misc Feature(14)order(1049..1051,1055..1057,1061..1063,1088..1090)
Misc Feature(15)1127..1129
Exon (1)1..216
Exon (2)217..301
Exon (3)302..455
Exon (4)456..526
Exon (5)527..654
Exon (6)655..712
Exon (7)713..811
Exon (8)812..942
Exon (9)943..1008
Exon (10)1009..1398
Translation

Target ORF information:

RefSeq Version NM_000631.4
Organism Homo sapiens(Human)
Definition Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000631.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGG ACATCGCGGT GGAGGAAGAT
CTCAGCAGCA CTCCCCTATT GAAAGACCTG CTGGAGCTCA CAAGGCGGGA GTTCCAGAGA
GAGGACATAG CTCTGAATTA CCGGGACGCT GAGGGGGATC TGGTTCGGCT GCTGTCGGAT
GAGGACGTAG CGCTCATGGT GCGGCAGGCT CGTGGCCTCC CCTCCCAGAA GCGCCTCTTC
CCCTGGAAGC TGCACATCAC GCAGAAGGAC AACTACAGGG TCTACAACAC GATGCCATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu18835
Clone ID Related Accession (Same CDS sequence) NM_013416.3
Accession Version NM_013416.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1047bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-06
Organism Homo sapiens(Human)
Product neutrophil cytosol factor 4 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC002798.1, AK290924.1 and BU076892.1. This sequence is a reference standard in the RefSeqGene project. On Dec 20, 2007 this sequence version replaced NM_013416.2. Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2). This isoform (2) lacks the PC (Phox and Cdc24p) motif, which may be important for the interaction with NCF2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002798.1, AK290924.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGT CTGTGGCCTG GGAGGGAGGG
GCCTGTCCAG CCTTCCTGCC ATCCCTACGA CCACTGCCCC TCACATCACC TTCTCATGGG
TCCCTCTCCC ACTCCAAAGC CCCCAGTGGC TCCCAGATGA GCCACAATGC TGTAACAAGC
CATCAACGTC CAGGGTGGCC TGGCCAGCCT CATTCCCCTT TCCCCCACCC CACACCCCAC
TTCCAGCCTG ATGCCTCCTT ACTCCAGCCT GTCACCCCCT TAGGGACATC GCGGTGGAGG
AAGATCTCAG CAGCACTCCC CTATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_038202.2
CDS185..1231
Misc Feature(1)122..124
Misc Feature(2)239..607
Misc Feature(3)order(281..283,287..292,296..298,362..364,596..598,605..607)
Misc Feature(4)order(356..364,458..463,497..499)
Misc Feature(5)356..364
Misc Feature(6)458..466
Misc Feature(7)644..646
Misc Feature(8)704..865
Misc Feature(9)order(719..724,734..736,746..748,791..796,800..805,842..844,851..853)
Misc Feature(10)order(782..784,821..826,830..832)
Misc Feature(11)896..>958
Exon (1)1..216
Exon (2)217..301
Exon (3)302..455
Exon (4)456..526
Exon (5)527..654
Exon (6)655..712
Exon (7)713..811
Exon (8)812..1253
Exon (9)1254..1643
Translation

Target ORF information:

RefSeq Version NM_013416.3
Organism Homo sapiens(Human)
Definition Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_013416.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGCTGTGG CCCAGCAGCT GCGGGCCGAG AGTGACTTTG AACAGCTTCC GGATGATGTT 
GCCATCTCGG CCAACATTGC TGACATCGAG GAGAAGAGAG GCTTCACCAG CCACTTTGTT
TTCGTCATCG AGGTGAAGAC AAAAGGAGGA TCCAAGTACC TCATCTACCG CCGCTACCGC
CAGTTCCATG CTTTGCAGAG CAAGCTGGAG GAGCGCTTCG GGCCAGACAG CAAGAGCAGT
GCCCTGGCCT GTACCCTGCC CACACTCCCA GCCAAAGTCT ACGTGGGTGT GAAACAGGAG
ATCGCCGAGA TGCGGATACC TGCCCTCAAC GCCTACATGA AGAGCCTGCT CAGCCTGCCG
GTCTGGGTGC TGATGGATGA GGACGTCCGG ATCTTCTTTT ACCAGTCGCC CTATGACTCA
GAGCAGGTGC CCCAGGCACT CCGCCGGCTC CGCCCGCGCA CCCGGAAAGT CAAGAGCGTG
TCCCCACAGG GCAACAGCGT TGACCGCATG GCAGCTCCGA GAGCAGAGGC TCTATTTGAC
TTCACTGGAA ACAGCAAACT GGAGCTGAAT TTCAAAGCTG GAGATGTGAT CTTCCTCCTC
AGTCGGATCA ACAAAGACTG GCTGGAGGGC ACTGTCCGGG GAGCCACGGG CATCTTCCCT
CTCTCCTTCG TGAAGATCCT CAAAGACTTC CCTGAGGAGG ACGACCCCAC CAACTGGCTG
CGTTGCTACT ACTACGAAGA CACCATCAGC ACCATCAAGT CTGTGGCCTG GGAGGGAGGG
GCCTGTCCAG CCTTCCTGCC ATCCCTACGA CCACTGCCCC TCACATCACC TTCTCATGGG
TCCCTCTCCC ACTCCAAAGC CCCCAGTGGC TCCCAGATGA GCCACAATGC TGTAACAAGC
CATCAACGTC CAGGGTGGCC TGGCCAGCCT CATTCCCCTT TCCCCCACCC CACACCCCAC
TTCCAGCCTG ATGCCTCCTT ACTCCAGCCT GTCACCCCCT TAGGGACATC GCGGTGGAGG
AAGATCTCAG CAGCACTCCC CTATTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu93501
Clone ID Related Accession (Same CDS sequence) XM_017028808.1
Accession Version XM_017028808.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 711bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product neutrophil cytosol factor 4 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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121
181
241
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361
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481
541
601
661
ATGCGGATAC CTGCCCTCAA CGCCTACATG AAGAGCCTGC TCAGCCTGCC GGTCTGGGTG 
CTGATGGATG AGGACGTCCG GATCTTCTTT TACCAGTCGC CCTATGACTC AGAGCAGGTG
CCCCAGGCAC TCCGCCGGCT CCGCCCGCGC ACCCGGAAAG TCAAGAGCGT GTCCCCACAG
GGCAACAGCG TTGACCGCAT GGCAGCTCCG AGAGCAGAGG CTCTATTTGA CTTCACTGGA
AACAGCAAAC TGGAGCTGAA TTTCAAAGCT GGAGATGTGA TCTTCCTCCT CAGTCGGATC
AACAAAGACT GGCTGGAGGG CACTGTCCGG GGAGCCACGG GCATCTTCCC TCTCTCCTTC
GTGAAGATCC TCAAAGACTT CCCTGAGGAG GACGACCCCA CCAACTGGCT GCGTTGCTAC
TACTACGAAG ACACCATCAG CACCATCAAG GACATCGCGG TGGAGGAAGA TCTCAGCAGC
ACTCCCCTAT TGAAAGACCT GCTGGAGCTC ACAAGGCGGG AGTTCCAGAG AGAGGACATA
GCTCTGAATT ACCGGGACGC TGAGGGGGAT CTGGTTCGGC TGCTGTCGGA TGAGGACGTA
GCGCTCATGG TGCGGCAGGC TCGTGGCCTC CCCTCCCAGA AGCGCCTCTT CCCCTGGAAG
CTGCACATCA CGCAGAAGGA CAACTACAGG GTCTACAACA CGATGCCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016884297.1
CDS858..1568
Translation

Target ORF information:

RefSeq Version XM_017028808.1
Organism Homo sapiens(Human)
Definition Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017028808.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGCGGATAC CTGCCCTCAA CGCCTACATG AAGAGCCTGC TCAGCCTGCC GGTCTGGGTG 
CTGATGGATG AGGACGTCCG GATCTTCTTT TACCAGTCGC CCTATGACTC AGAGCAGGTG
CCCCAGGCAC TCCGCCGGCT CCGCCCGCGC ACCCGGAAAG TCAAGAGCGT GTCCCCACAG
GGCAACAGCG TTGACCGCAT GGCAGCTCCG AGAGCAGAGG CTCTATTTGA CTTCACTGGA
AACAGCAAAC TGGAGCTGAA TTTCAAAGCT GGAGATGTGA TCTTCCTCCT CAGTCGGATC
AACAAAGACT GGCTGGAGGG CACTGTCCGG GGAGCCACGG GCATCTTCCC TCTCTCCTTC
GTGAAGATCC TCAAAGACTT CCCTGAGGAG GACGACCCCA CCAACTGGCT GCGTTGCTAC
TACTACGAAG ACACCATCAG CACCATCAAG GACATCGCGG TGGAGGAAGA TCTCAGCAGC
ACTCCCCTAT TGAAAGACCT GCTGGAGCTC ACAAGGCGGG AGTTCCAGAG AGAGGACATA
GCTCTGAATT ACCGGGACGC TGAGGGGGAT CTGGTTCGGC TGCTGTCGGA TGAGGACGTA
GCGCTCATGG TGCGGCAGGC TCGTGGCCTC CCCTCCCAGA AGCGCCTCTT CCCCTGGAAG
CTGCACATCA CGCAGAAGGA CAACTACAGG GTCTACAACA CGATGCCATG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Association between NCF4 rs4821544T/C polymorphism and inflammatory bowel disease risk in Caucasian: a meta-analysis.
Inflammation research : official journal of the European Histamine Research Society ... [et al.]64(10)825-31(2015 Oct)
Wu PB,Dai JF,Wang Q,Zhang G,Tan SY,Li M,Ye HL


book

Activation of NADPH oxidase subunit NCF4 induces ROS-mediated EMT signaling in HeLa cells.
Cellular signalling26(4)784-96(2014 Apr)
Kim YM,Cho M


book

Germline variation in NCF4, an innate immunity gene, is associated with an increased risk of colorectal cancer.
International journal of cancer134(6)1399-407(2014 Mar)
Ryan BM,Zanetti KA,Robles AI,Schetter AJ,Goodman J,Hayes RB,Huang WY,Gunter MJ,Yeager M,Burdette L,Berndt SI,Harris CC


book

Association of anthracycline-related cardiac histological lesions with NADPH oxidase functional polymorphisms.
The oncologist18(4)446-53(2013)
Cascales A,Pastor-Quirante F,Sánchez-Vega B,Luengo-Gil G,Corral J,Ortuño-Pacheco G,Vicente V,de la Peña FA


book

Cutting edge: NADPH oxidase modulates MHC class II antigen presentation by B cells.
Journal of immunology (Baltimore, Md. : 1950)189(8)3800-4(2012 Oct)
Crotzer VL,Matute JD,Arias AA,Zhao H,Quilliam LA,Dinauer MC,Blum JS


 
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