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NFE2L1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol NFE2L1
Entrez Gene ID 4779
Full Name nuclear factor, erythroid 2 like 1
Synonyms LCR-F1,NRF1,TCF11
General protein information
Preferred Names

nuclear factor, erythroid 2 like 1

Names

nuclear factor erythroid 2-related factor 1
NF-E2-related factor 1
NFE2-related factor 1
TCF-11
locus control region-factor 1
nuclear factor, erythroid derived 2, like 1
transcription factor 11 (basic leucine zipper type)
transcription factor HBZ17
transcription factor LCR-F1

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

17

17q21.32

Disorder MIM:

163260


mRNA and Protein(s)

mRNA Protein Name
XM_005257410.3 XP_005257467.1 nuclear factor erythroid 2-related factor 1 isoform X1
NM_001330261.1 NP_001317190.1 nuclear factor erythroid 2-related factor 1 isoform 2
XM_005257410.2 XP_005257467.1 nuclear factor erythroid 2-related factor 1 isoform X1
NM_001330262.1 NP_001317191.1 nuclear factor erythroid 2-related factor 1 isoform 3
NM_003204.2 NP_003195.1 nuclear factor erythroid 2-related factor 1 isoform 1
XM_005257411.3 XP_005257468.1 nuclear factor erythroid 2-related factor 1 isoform X2
XM_005257415.3 XP_005257472.1 nuclear factor erythroid 2-related factor 1 isoform X4
XM_005257412.3 XP_005257469.1 nuclear factor erythroid 2-related factor 1 isoform X3



Rattus norvegicus (Norway rat) Nfe2l1 XP_006247299.1
Xenopus tropicalis (tropical clawed frog) nfe2l1 XP_002938061.2
Macaca mulatta (rhesus monkey) NFE2L1 XP_001087083.1
Gallus gallus (chicken) NFE2L1 NP_001025927.1
Danio rerio (zebrafish) nfe2l1b NP_001265771.1
Pan troglodytes (chimpanzee) NFE2L1 XP_001173202.1
Canis lupus familiaris (dog) NFE2L1 XP_005624669.1
Mus musculus (house mouse) Nfe2l1 NP_001123922.1
Homo sapiens (human) NFE2L1 NP_003195.1
Bos taurus (cattle) NFE2L1 NP_001095985.1


Related articles in PubMed

Transcription factor Nrf1 is negatively regulated by its O-GlcNAcylation status.
Chen J, Liu X, Lü F, Liu X, Ru Y, Ren Y, Yao L, Zhang Y
FEBS letters589(18)2347-58(2015 Aug)

p97-dependent retrotranslocation and proteolytic processing govern formation of active Nrf1 upon proteasome inhibition.
Radhakrishnan SK, den Besten W, Deshaies RJ
eLife3e01856(2014)

The casein kinase 2-nrf1 axis controls the clearance of ubiquitinated proteins by regulating proteasome gene expression.
Tsuchiya Y, Taniguchi H, Ito Y, Morita T, Karim MR, Ohtake N, Fukagai K, Ito T, Okamuro S, Iemura S, Natsume T, Nishida E, Kobayashi A
Molecular and cellular biology33(17)3461-72(2013 Sep)

Glycogen synthase kinase 3 regulates expression of nuclear factor-erythroid-2 related transcription factor-1 (Nrf1) and inhibits pro-survival function of Nrf1.
Biswas M, Kwong EK, Park E, Nagra P, Chan JY
Experimental cell research319(13)1922-31(2013 Aug)

Human small Maf proteins form heterodimers with CNC family transcription factors and recognize the NF-E2 motif.
Toki T, Itoh J, Kitazawa J, Arai K, Hatakeyama K, Akasaka J, Igarashi K, Nomura N, Yokoyama M, Yamamoto M, Ito E
Oncogene14(16)1901-10(1997 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our investigation shows that the ER membrane protein TCF11/Nrf1 is an essential component of the cellular stress response mechanism. In response to cytotoxic stress, TCF11/Nrf1 is retrotranslocated and transferred to the nucleus where it induces proteasome subunit expression via binding to the ARE region of the relevant promoter.
Title: TCF11/Nrf1-Mediated Induction of Proteasome Expression Prevents Cytotoxicity by Rotenone.

Nrf1 is negatively regulated by its O-GlcNAcylation status that depends on the glucose concentrations
Title: Transcription factor Nrf1 is negatively regulated by its O-GlcNAcylation status.

Data uncover an unexpected role for p97 in activation of transcription factor Nrf1 by relocalizing it from the endoplasmic reticulum lumen to the cytosol.
Title: p97-dependent retrotranslocation and proteolytic processing govern formation of active Nrf1 upon proteasome inhibition.

The study identifies casein kinase 2 as an NRF1-binding protein and finds that the knockdown of casein kinase 2 enhances the NRF1-dependent expression of the proteasome subunit genes.
Title: The casein kinase 2-nrf1 axis controls the clearance of ubiquitinated proteins by regulating proteasome gene expression.

GSK3 regulates Nrf1 expression and cell survival function in response to stress activation.
Title: Glycogen synthase kinase 3 regulates expression of nuclear factor-erythroid-2 related transcription factor-1 (Nrf1) and inhibits pro-survival function of Nrf1.

The following NFE2L1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFE2L1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu13588
NM_003204.2
Latest version!
Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu13588
XM_005257410.3
Latest version!
Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
OHu50435 NM_001330261.1
Latest version!
Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu50436 NM_001330262.1
Latest version!
Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu50435 XM_005257411.3
Latest version!
Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu50436 XM_005257412.3
Latest version!
Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu50437 XM_005257415.3
Latest version!
Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
View Old Accession Versions >>
OHu13588
XM_005257410.2 Homo sapiens nuclear factor, erythroid 2-like 1 (NFE2L1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$279.50-$391.30
$559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu13588
Clone ID Related Accession (Same CDS sequence) XM_005257410.3 , XM_005257410.2 , NM_003204.2
Accession Version NM_003204.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-06
Organism Homo sapiens(Human)
Product nuclear factor erythroid 2-related factor 1 isoform 1
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB097304.1, DA230932.1, AK090459.1, U08853.1, AC004477.2 and BM983473.1. On May 30, 2008 this sequence version replaced NM_003204.1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK090459.1, X77366.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GACATAGATC TGATTGACAT CCTTTGGCGA
CAGGATATTG ATCTGGGGGC TGGGCGTGAG GTTTTTGACT ATAGTCACCG CCAGAAGGAG
CAGGATGTGG AGAAGGAGCT GCGAGATGGA GGCGAGCAGG ACACCTGGGC AGGCGAGGGC
GCGGAAGCTC TGGCACGGAA CCTGCTAGTG GATGGAGAGA CTGGGGAGAG CTTCCCTGCA
CAGGTGCCTA GTGGGGAGGA CCAGACGGCC CTGTCCCTGG AAGAGTGCCT TAGGCTGCTG
GAAGCCACCT GCCCCTTTGG GGAGAATGCT GAGTTTCCAG CAGACATTTC CAGCATAACA
GAAGCAGTGC CTAGTGAGAG TGAGCCCCCT GCTCTTCAAA ACAACCTCTT GTCTCCTCTT
CTGACCGGGA CAGAGTCACC ATTTGATTTG GAACAGCAGT GGCAAGATCT CATGTCCATC
ATGGAAATGC AGGCCATGGA AGTGAACACA TCAGCAAGTG AAATCCTGTA CAGTGCCCCT
CCTGGAGACC CACTGAGCAC CAACTACAGC CTTGCCCCCA ACACTCCCAT CAATCAGAAT
GTCAGCCTGC ATCAGGCGTC CCTGGGGGGC TGCAGCCAGG ACTTCTTACT CTTCAGCCCC
GAGGTGGAAA GCCTGCCTGT GGCCAGTAGC TCCACGCTGC TCCCGTTGGC CCCCAGCAAT
TCTACCAGCC TCAACTCCAC CTTCGGCTCC ACCAACCTGA CAGGGCTCTT CTTTCCACCC
CAGCTCAATG GCACAGCCAA TGACACAGCA GGCCCAGAGC TGCCTGACCC TTTGGGGGGT
CTGTTAGATG AAGCTATGTT GGATGAGATC AGCCTTATGG ACCTGGCCAT TGAAGAAGGC
TTTAACCCTG TGCAGGCCTC CCAGCTGGAG GAGGAATTTG ACTCTGACTC AGGCCTTTCC
TTAGACTCGA GCCATAGCCC TTCTTCCCTA AGCAGCTCTG AAGGCAGTTC TTCCTCTTCT
TCCTCCTCCT CTTCCTCTTC TTCCTCTGCT TCTTCCTCTG CCTCTTCCTC CTTTTCTGAG
GAAGGTGCGG TTGGCTACAG CTCTGACTCT GAGACCCTGG ATCTGGAAGA GGCCGAGGGT
GCTGTGGGCT ACCAGCCTGA GTATTCCAAG TTCTGCCGCA TGAGCTACCA GGATCCAGCT
CAGCTCTCAT GCCTGCCCTA CCTGGAGCAC GTGGGCCACA ACCACACATA CAACATGGCA
CCCAGTGCCC TGGACTCAGC CGACCTGCCA CCACCCAGTG CCCTCAAGAA AGGCAGCAAG
GAGAAGCAGG CTGACTTCCT GGACAAGCAG ATGAGCCGGG ATGAGCACCG AGCCCGAGCC
ATGAAGATCC CTTTCACCAA TGACAAAATC ATCAACCTGC CTGTGGAGGA GTTCAATGAA
CTGCTGTCCA AATACCAGTT GAGTGAAGCC CAGCTGAGCC TCATCCGAGA CATCCGGCGC
CGGGGCAAGA ACAAGATGGC GGCGCAGAAC TGCCGCAAGC GCAAGCTGGA CACCATCCTG
AATCTGGAGC GTGATGTGGA GGACCTGCAG CGTGACAAAG CCCGGCTGCT GCGGGAGAAA
GTGGAGTTCC TGCGCTCCCT GCGACAGATG AAGCAGAAGG TCCAGAGCCT GTACCAGGAG
GTGTTTGGGC GGCTGCGAGA TGAGAACGGA CGACCCTACT CGCCCAGTCA GTATGCGCTC
CAGTACGCCG GGGACGGCAG TGTCCTCCTC ATCCCCCGCA CGATGGCCGA CCAGCAGGCC
CGGCGGCAGG AGAGGAAGCC AAAGGACCGG AGAAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_003195.1
CDS652..2970
Misc Feature(1)592..594
Misc Feature(2)670..723
Misc Feature(3)2602..2805
Misc Feature(4)2605..2793
Misc Feature(5)2617..2676
Misc Feature(6)order(2629..2634,2638..2646,2650..2667,2671..2679)
Misc Feature(7)order(2674..2676,2683..2688,2695..2700,2704..2709,2716..2721,2725..2730,2737..2742,2746..2751,2758..2763,2767..2772)
Misc Feature(8)2695..2739
Exon (1)1..139
Exon (2)140..1161
Exon (3)1162..1374
Exon (4)1375..1464
Exon (5)1465..1623
Exon (6)1624..4874
Translation

Target ORF information:

RefSeq Version NM_003204.2
Organism Homo sapiens(Human)
Definition Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_003204.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GACATAGATC TGATTGACAT CCTTTGGCGA
CAGGATATTG ATCTGGGGGC TGGGCGTGAG GTTTTTGACT ATAGTCACCG CCAGAAGGAG
CAGGATGTGG AGAAGGAGCT GCGAGATGGA GGCGAGCAGG ACACCTGGGC AGGCGAGGGC
GCGGAAGCTC TGGCACGGAA CCTGCTAGTG GATGGAGAGA CTGGGGAGAG CTTCCCTGCA
CAGGTGCCTA GTGGGGAGGA CCAGACGGCC CTGTCCCTGG AAGAGTGCCT TAGGCTGCTG
GAAGCCACCT GCCCCTTTGG GGAGAATGCT GAGTTTCCAG CAGACATTTC CAGCATAACA
GAAGCAGTGC CTAGTGAGAG TGAGCCCCCT GCTCTTCAAA ACAACCTCTT GTCTCCTCTT
CTGACCGGGA CAGAGTCACC ATTTGATTTG GAACAGCAGT GGCAAGATCT CATGTCCATC
ATGGAAATGC AGGCCATGGA AGTGAACACA TCAGCAAGTG AAATCCTGTA CAGTGCCCCT
CCTGGAGACC CACTGAGCAC CAACTACAGC CTTGCCCCCA ACACTCCCAT CAATCAGAAT
GTCAGCCTGC ATCAGGCGTC CCTGGGGGGC TGCAGCCAGG ACTTCTTACT CTTCAGCCCC
GAGGTGGAAA GCCTGCCTGT GGCCAGTAGC TCCACGCTGC TCCCGTTGGC CCCCAGCAAT
TCTACCAGCC TCAACTCCAC CTTCGGCTCC ACCAACCTGA CAGGGCTCTT CTTTCCACCC
CAGCTCAATG GCACAGCCAA TGACACAGCA GGCCCAGAGC TGCCTGACCC TTTGGGGGGT
CTGTTAGATG AAGCTATGTT GGATGAGATC AGCCTTATGG ACCTGGCCAT TGAAGAAGGC
TTTAACCCTG TGCAGGCCTC CCAGCTGGAG GAGGAATTTG ACTCTGACTC AGGCCTTTCC
TTAGACTCGA GCCATAGCCC TTCTTCCCTA AGCAGCTCTG AAGGCAGTTC TTCCTCTTCT
TCCTCCTCCT CTTCCTCTTC TTCCTCTGCT TCTTCCTCTG CCTCTTCCTC CTTTTCTGAG
GAAGGTGCGG TTGGCTACAG CTCTGACTCT GAGACCCTGG ATCTGGAAGA GGCCGAGGGT
GCTGTGGGCT ACCAGCCTGA GTATTCCAAG TTCTGCCGCA TGAGCTACCA GGATCCAGCT
CAGCTCTCAT GCCTGCCCTA CCTGGAGCAC GTGGGCCACA ACCACACATA CAACATGGCA
CCCAGTGCCC TGGACTCAGC CGACCTGCCA CCACCCAGTG CCCTCAAGAA AGGCAGCAAG
GAGAAGCAGG CTGACTTCCT GGACAAGCAG ATGAGCCGGG ATGAGCACCG AGCCCGAGCC
ATGAAGATCC CTTTCACCAA TGACAAAATC ATCAACCTGC CTGTGGAGGA GTTCAATGAA
CTGCTGTCCA AATACCAGTT GAGTGAAGCC CAGCTGAGCC TCATCCGAGA CATCCGGCGC
CGGGGCAAGA ACAAGATGGC GGCGCAGAAC TGCCGCAAGC GCAAGCTGGA CACCATCCTG
AATCTGGAGC GTGATGTGGA GGACCTGCAG CGTGACAAAG CCCGGCTGCT GCGGGAGAAA
GTGGAGTTCC TGCGCTCCCT GCGACAGATG AAGCAGAAGG TCCAGAGCCT GTACCAGGAG
GTGTTTGGGC GGCTGCGAGA TGAGAACGGA CGACCCTACT CGCCCAGTCA GTATGCGCTC
CAGTACGCCG GGGACGGCAG TGTCCTCCTC ATCCCCCGCA CGATGGCCGA CCAGCAGGCC
CGGCGGCAGG AGAGGAAGCC AAAGGACCGG AGAAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13588
Clone ID Related Accession (Same CDS sequence) XM_005257410.3 , XM_005257410.2 , NM_003204.2
Accession Version XM_005257410.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product nuclear factor erythroid 2-related factor 1 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257410.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GACATAGATC TGATTGACAT CCTTTGGCGA
CAGGATATTG ATCTGGGGGC TGGGCGTGAG GTTTTTGACT ATAGTCACCG CCAGAAGGAG
CAGGATGTGG AGAAGGAGCT GCGAGATGGA GGCGAGCAGG ACACCTGGGC AGGCGAGGGC
GCGGAAGCTC TGGCACGGAA CCTGCTAGTG GATGGAGAGA CTGGGGAGAG CTTCCCTGCA
CAGGTGCCTA GTGGGGAGGA CCAGACGGCC CTGTCCCTGG AAGAGTGCCT TAGGCTGCTG
GAAGCCACCT GCCCCTTTGG GGAGAATGCT GAGTTTCCAG CAGACATTTC CAGCATAACA
GAAGCAGTGC CTAGTGAGAG TGAGCCCCCT GCTCTTCAAA ACAACCTCTT GTCTCCTCTT
CTGACCGGGA CAGAGTCACC ATTTGATTTG GAACAGCAGT GGCAAGATCT CATGTCCATC
ATGGAAATGC AGGCCATGGA AGTGAACACA TCAGCAAGTG AAATCCTGTA CAGTGCCCCT
CCTGGAGACC CACTGAGCAC CAACTACAGC CTTGCCCCCA ACACTCCCAT CAATCAGAAT
GTCAGCCTGC ATCAGGCGTC CCTGGGGGGC TGCAGCCAGG ACTTCTTACT CTTCAGCCCC
GAGGTGGAAA GCCTGCCTGT GGCCAGTAGC TCCACGCTGC TCCCGTTGGC CCCCAGCAAT
TCTACCAGCC TCAACTCCAC CTTCGGCTCC ACCAACCTGA CAGGGCTCTT CTTTCCACCC
CAGCTCAATG GCACAGCCAA TGACACAGCA GGCCCAGAGC TGCCTGACCC TTTGGGGGGT
CTGTTAGATG AAGCTATGTT GGATGAGATC AGCCTTATGG ACCTGGCCAT TGAAGAAGGC
TTTAACCCTG TGCAGGCCTC CCAGCTGGAG GAGGAATTTG ACTCTGACTC AGGCCTTTCC
TTAGACTCGA GCCATAGCCC TTCTTCCCTA AGCAGCTCTG AAGGCAGTTC TTCCTCTTCT
TCCTCCTCCT CTTCCTCTTC TTCCTCTGCT TCTTCCTCTG CCTCTTCCTC CTTTTCTGAG
GAAGGTGCGG TTGGCTACAG CTCTGACTCT GAGACCCTGG ATCTGGAAGA GGCCGAGGGT
GCTGTGGGCT ACCAGCCTGA GTATTCCAAG TTCTGCCGCA TGAGCTACCA GGATCCAGCT
CAGCTCTCAT GCCTGCCCTA CCTGGAGCAC GTGGGCCACA ACCACACATA CAACATGGCA
CCCAGTGCCC TGGACTCAGC CGACCTGCCA CCACCCAGTG CCCTCAAGAA AGGCAGCAAG
GAGAAGCAGG CTGACTTCCT GGACAAGCAG ATGAGCCGGG ATGAGCACCG AGCCCGAGCC
ATGAAGATCC CTTTCACCAA TGACAAAATC ATCAACCTGC CTGTGGAGGA GTTCAATGAA
CTGCTGTCCA AATACCAGTT GAGTGAAGCC CAGCTGAGCC TCATCCGAGA CATCCGGCGC
CGGGGCAAGA ACAAGATGGC GGCGCAGAAC TGCCGCAAGC GCAAGCTGGA CACCATCCTG
AATCTGGAGC GTGATGTGGA GGACCTGCAG CGTGACAAAG CCCGGCTGCT GCGGGAGAAA
GTGGAGTTCC TGCGCTCCCT GCGACAGATG AAGCAGAAGG TCCAGAGCCT GTACCAGGAG
GTGTTTGGGC GGCTGCGAGA TGAGAACGGA CGACCCTACT CGCCCAGTCA GTATGCGCTC
CAGTACGCCG GGGACGGCAG TGTCCTCCTC ATCCCCCGCA CGATGGCCGA CCAGCAGGCC
CGGCGGCAGG AGAGGAAGCC AAAGGACCGG AGAAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257467.1
CDS598..2916
Misc Feature(1)2548..2751
Misc Feature(2)2551..2739
Misc Feature(3)order(2575..2580,2584..2592,2596..2613,2617..2625)
Misc Feature(4)order(2620..2622,2629..2634,2641..2646,2650..2655,2662..2667,2671..2676,2683..2688,2692..2697,2704..2709,2713..2718)
Translation

Target ORF information:

RefSeq Version XM_005257410.3
Organism Homo sapiens(Human)
Definition Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257410.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GACATAGATC TGATTGACAT CCTTTGGCGA
CAGGATATTG ATCTGGGGGC TGGGCGTGAG GTTTTTGACT ATAGTCACCG CCAGAAGGAG
CAGGATGTGG AGAAGGAGCT GCGAGATGGA GGCGAGCAGG ACACCTGGGC AGGCGAGGGC
GCGGAAGCTC TGGCACGGAA CCTGCTAGTG GATGGAGAGA CTGGGGAGAG CTTCCCTGCA
CAGGTGCCTA GTGGGGAGGA CCAGACGGCC CTGTCCCTGG AAGAGTGCCT TAGGCTGCTG
GAAGCCACCT GCCCCTTTGG GGAGAATGCT GAGTTTCCAG CAGACATTTC CAGCATAACA
GAAGCAGTGC CTAGTGAGAG TGAGCCCCCT GCTCTTCAAA ACAACCTCTT GTCTCCTCTT
CTGACCGGGA CAGAGTCACC ATTTGATTTG GAACAGCAGT GGCAAGATCT CATGTCCATC
ATGGAAATGC AGGCCATGGA AGTGAACACA TCAGCAAGTG AAATCCTGTA CAGTGCCCCT
CCTGGAGACC CACTGAGCAC CAACTACAGC CTTGCCCCCA ACACTCCCAT CAATCAGAAT
GTCAGCCTGC ATCAGGCGTC CCTGGGGGGC TGCAGCCAGG ACTTCTTACT CTTCAGCCCC
GAGGTGGAAA GCCTGCCTGT GGCCAGTAGC TCCACGCTGC TCCCGTTGGC CCCCAGCAAT
TCTACCAGCC TCAACTCCAC CTTCGGCTCC ACCAACCTGA CAGGGCTCTT CTTTCCACCC
CAGCTCAATG GCACAGCCAA TGACACAGCA GGCCCAGAGC TGCCTGACCC TTTGGGGGGT
CTGTTAGATG AAGCTATGTT GGATGAGATC AGCCTTATGG ACCTGGCCAT TGAAGAAGGC
TTTAACCCTG TGCAGGCCTC CCAGCTGGAG GAGGAATTTG ACTCTGACTC AGGCCTTTCC
TTAGACTCGA GCCATAGCCC TTCTTCCCTA AGCAGCTCTG AAGGCAGTTC TTCCTCTTCT
TCCTCCTCCT CTTCCTCTTC TTCCTCTGCT TCTTCCTCTG CCTCTTCCTC CTTTTCTGAG
GAAGGTGCGG TTGGCTACAG CTCTGACTCT GAGACCCTGG ATCTGGAAGA GGCCGAGGGT
GCTGTGGGCT ACCAGCCTGA GTATTCCAAG TTCTGCCGCA TGAGCTACCA GGATCCAGCT
CAGCTCTCAT GCCTGCCCTA CCTGGAGCAC GTGGGCCACA ACCACACATA CAACATGGCA
CCCAGTGCCC TGGACTCAGC CGACCTGCCA CCACCCAGTG CCCTCAAGAA AGGCAGCAAG
GAGAAGCAGG CTGACTTCCT GGACAAGCAG ATGAGCCGGG ATGAGCACCG AGCCCGAGCC
ATGAAGATCC CTTTCACCAA TGACAAAATC ATCAACCTGC CTGTGGAGGA GTTCAATGAA
CTGCTGTCCA AATACCAGTT GAGTGAAGCC CAGCTGAGCC TCATCCGAGA CATCCGGCGC
CGGGGCAAGA ACAAGATGGC GGCGCAGAAC TGCCGCAAGC GCAAGCTGGA CACCATCCTG
AATCTGGAGC GTGATGTGGA GGACCTGCAG CGTGACAAAG CCCGGCTGCT GCGGGAGAAA
GTGGAGTTCC TGCGCTCCCT GCGACAGATG AAGCAGAAGG TCCAGAGCCT GTACCAGGAG
GTGTTTGGGC GGCTGCGAGA TGAGAACGGA CGACCCTACT CGCCCAGTCA GTATGCGCTC
CAGTACGCCG GGGACGGCAG TGTCCTCCTC ATCCCCCGCA CGATGGCCGA CCAGCAGGCC
CGGCGGCAGG AGAGGAAGCC AAAGGACCGG AGAAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu13588
Clone ID Related Accession (Same CDS sequence) XM_005257410.3 , XM_005257410.2 , NM_003204.2
Accession Version XM_005257410.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-12
Organism Homo sapiens(Human)
Product nuclear factor erythroid 2-related factor 1 isoform X1
Comment

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GACATAGATC TGATTGACAT CCTTTGGCGA
CAGGATATTG ATCTGGGGGC TGGGCGTGAG GTTTTTGACT ATAGTCACCG CCAGAAGGAG
CAGGATGTGG AGAAGGAGCT GCGAGATGGA GGCGAGCAGG ACACCTGGGC AGGCGAGGGC
GCGGAAGCTC TGGCACGGAA CCTGCTAGTG GATGGAGAGA CTGGGGAGAG CTTCCCTGCA
CAGGTGCCTA GTGGGGAGGA CCAGACGGCC CTGTCCCTGG AAGAGTGCCT TAGGCTGCTG
GAAGCCACCT GCCCCTTTGG GGAGAATGCT GAGTTTCCAG CAGACATTTC CAGCATAACA
GAAGCAGTGC CTAGTGAGAG TGAGCCCCCT GCTCTTCAAA ACAACCTCTT GTCTCCTCTT
CTGACCGGGA CAGAGTCACC ATTTGATTTG GAACAGCAGT GGCAAGATCT CATGTCCATC
ATGGAAATGC AGGCCATGGA AGTGAACACA TCAGCAAGTG AAATCCTGTA CAGTGCCCCT
CCTGGAGACC CACTGAGCAC CAACTACAGC CTTGCCCCCA ACACTCCCAT CAATCAGAAT
GTCAGCCTGC ATCAGGCGTC CCTGGGGGGC TGCAGCCAGG ACTTCTTACT CTTCAGCCCC
GAGGTGGAAA GCCTGCCTGT GGCCAGTAGC TCCACGCTGC TCCCGTTGGC CCCCAGCAAT
TCTACCAGCC TCAACTCCAC CTTCGGCTCC ACCAACCTGA CAGGGCTCTT CTTTCCACCC
CAGCTCAATG GCACAGCCAA TGACACAGCA GGCCCAGAGC TGCCTGACCC TTTGGGGGGT
CTGTTAGATG AAGCTATGTT GGATGAGATC AGCCTTATGG ACCTGGCCAT TGAAGAAGGC
TTTAACCCTG TGCAGGCCTC CCAGCTGGAG GAGGAATTTG ACTCTGACTC AGGCCTTTCC
TTAGACTCGA GCCATAGCCC TTCTTCCCTA AGCAGCTCTG AAGGCAGTTC TTCCTCTTCT
TCCTCCTCCT CTTCCTCTTC TTCCTCTGCT TCTTCCTCTG CCTCTTCCTC CTTTTCTGAG
GAAGGTGCGG TTGGCTACAG CTCTGACTCT GAGACCCTGG ATCTGGAAGA GGCCGAGGGT
GCTGTGGGCT ACCAGCCTGA GTATTCCAAG TTCTGCCGCA TGAGCTACCA GGATCCAGCT
CAGCTCTCAT GCCTGCCCTA CCTGGAGCAC GTGGGCCACA ACCACACATA CAACATGGCA
CCCAGTGCCC TGGACTCAGC CGACCTGCCA CCACCCAGTG CCCTCAAGAA AGGCAGCAAG
GAGAAGCAGG CTGACTTCCT GGACAAGCAG ATGAGCCGGG ATGAGCACCG AGCCCGAGCC
ATGAAGATCC CTTTCACCAA TGACAAAATC ATCAACCTGC CTGTGGAGGA GTTCAATGAA
CTGCTGTCCA AATACCAGTT GAGTGAAGCC CAGCTGAGCC TCATCCGAGA CATCCGGCGC
CGGGGCAAGA ACAAGATGGC GGCGCAGAAC TGCCGCAAGC GCAAGCTGGA CACCATCCTG
AATCTGGAGC GTGATGTGGA GGACCTGCAG CGTGACAAAG CCCGGCTGCT GCGGGAGAAA
GTGGAGTTCC TGCGCTCCCT GCGACAGATG AAGCAGAAGG TCCAGAGCCT GTACCAGGAG
GTGTTTGGGC GGCTGCGAGA TGAGAACGGA CGACCCTACT CGCCCAGTCA GTATGCGCTC
CAGTACGCCG GGGACGGCAG TGTCCTCCTC ATCCCCCGCA CGATGGCCGA CCAGCAGGCC
CGGCGGCAGG AGAGGAAGCC AAAGGACCGG AGAAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257467.1
Translation

Target ORF information:

RefSeq Version XM_005257410.2
Organism Homo sapiens(Human)
Definition Homo sapiens nuclear factor, erythroid 2-like 1 (NFE2L1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257410.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GACATAGATC TGATTGACAT CCTTTGGCGA
CAGGATATTG ATCTGGGGGC TGGGCGTGAG GTTTTTGACT ATAGTCACCG CCAGAAGGAG
CAGGATGTGG AGAAGGAGCT GCGAGATGGA GGCGAGCAGG ACACCTGGGC AGGCGAGGGC
GCGGAAGCTC TGGCACGGAA CCTGCTAGTG GATGGAGAGA CTGGGGAGAG CTTCCCTGCA
CAGGTGCCTA GTGGGGAGGA CCAGACGGCC CTGTCCCTGG AAGAGTGCCT TAGGCTGCTG
GAAGCCACCT GCCCCTTTGG GGAGAATGCT GAGTTTCCAG CAGACATTTC CAGCATAACA
GAAGCAGTGC CTAGTGAGAG TGAGCCCCCT GCTCTTCAAA ACAACCTCTT GTCTCCTCTT
CTGACCGGGA CAGAGTCACC ATTTGATTTG GAACAGCAGT GGCAAGATCT CATGTCCATC
ATGGAAATGC AGGCCATGGA AGTGAACACA TCAGCAAGTG AAATCCTGTA CAGTGCCCCT
CCTGGAGACC CACTGAGCAC CAACTACAGC CTTGCCCCCA ACACTCCCAT CAATCAGAAT
GTCAGCCTGC ATCAGGCGTC CCTGGGGGGC TGCAGCCAGG ACTTCTTACT CTTCAGCCCC
GAGGTGGAAA GCCTGCCTGT GGCCAGTAGC TCCACGCTGC TCCCGTTGGC CCCCAGCAAT
TCTACCAGCC TCAACTCCAC CTTCGGCTCC ACCAACCTGA CAGGGCTCTT CTTTCCACCC
CAGCTCAATG GCACAGCCAA TGACACAGCA GGCCCAGAGC TGCCTGACCC TTTGGGGGGT
CTGTTAGATG AAGCTATGTT GGATGAGATC AGCCTTATGG ACCTGGCCAT TGAAGAAGGC
TTTAACCCTG TGCAGGCCTC CCAGCTGGAG GAGGAATTTG ACTCTGACTC AGGCCTTTCC
TTAGACTCGA GCCATAGCCC TTCTTCCCTA AGCAGCTCTG AAGGCAGTTC TTCCTCTTCT
TCCTCCTCCT CTTCCTCTTC TTCCTCTGCT TCTTCCTCTG CCTCTTCCTC CTTTTCTGAG
GAAGGTGCGG TTGGCTACAG CTCTGACTCT GAGACCCTGG ATCTGGAAGA GGCCGAGGGT
GCTGTGGGCT ACCAGCCTGA GTATTCCAAG TTCTGCCGCA TGAGCTACCA GGATCCAGCT
CAGCTCTCAT GCCTGCCCTA CCTGGAGCAC GTGGGCCACA ACCACACATA CAACATGGCA
CCCAGTGCCC TGGACTCAGC CGACCTGCCA CCACCCAGTG CCCTCAAGAA AGGCAGCAAG
GAGAAGCAGG CTGACTTCCT GGACAAGCAG ATGAGCCGGG ATGAGCACCG AGCCCGAGCC
ATGAAGATCC CTTTCACCAA TGACAAAATC ATCAACCTGC CTGTGGAGGA GTTCAATGAA
CTGCTGTCCA AATACCAGTT GAGTGAAGCC CAGCTGAGCC TCATCCGAGA CATCCGGCGC
CGGGGCAAGA ACAAGATGGC GGCGCAGAAC TGCCGCAAGC GCAAGCTGGA CACCATCCTG
AATCTGGAGC GTGATGTGGA GGACCTGCAG CGTGACAAAG CCCGGCTGCT GCGGGAGAAA
GTGGAGTTCC TGCGCTCCCT GCGACAGATG AAGCAGAAGG TCCAGAGCCT GTACCAGGAG
GTGTTTGGGC GGCTGCGAGA TGAGAACGGA CGACCCTACT CGCCCAGTCA GTATGCGCTC
CAGTACGCCG GGGACGGCAG TGTCCTCCTC ATCCCCCGCA CGATGGCCGA CCAGCAGGCC
CGGCGGCAGG AGAGGAAGCC AAAGGACCGG AGAAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50435
Clone ID Related Accession (Same CDS sequence) NM_001330261.1 , XM_005257411.3
Accession Version NM_001330261.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-09
Organism Homo sapiens(Human)
Product nuclear factor erythroid 2-related factor 1 isoform 2
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004477.2 and BM971272.1. On Aug 20, 2016 this sequence version replaced XM_017024691.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.45841.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GATATTGATC TGGGGGCTGG GCGTGAGGTT
TTTGACTATA GTCACCGCCA GAAGGAGCAG GATGTGGAGA AGGAGCTGCG AGATGGAGGC
GAGCAGGACA CCTGGGCAGG CGAGGGCGCG GAAGCTCTGG CACGGAACCT GCTAGTGGAT
GGAGAGACTG GGGAGAGCTT CCCTGCACAG GTGCCTAGTG GGGAGGACCA GACGGCCCTG
TCCCTGGAAG AGTGCCTTAG GCTGCTGGAA GCCACCTGCC CCTTTGGGGA GAATGCTGAG
TTTCCAGCAG ACATTTCCAG CATAACAGAA GCAGTGCCTA GTGAGAGTGA GCCCCCTGCT
CTTCAAAACA ACCTCTTGTC TCCTCTTCTG ACCGGGACAG AGTCACCATT TGATTTGGAA
CAGCAGTGGC AAGATCTCAT GTCCATCATG GAAATGCAGG CCATGGAAGT GAACACATCA
GCAAGTGAAA TCCTGTACAG TGCCCCTCCT GGAGACCCAC TGAGCACCAA CTACAGCCTT
GCCCCCAACA CTCCCATCAA TCAGAATGTC AGCCTGCATC AGGCGTCCCT GGGGGGCTGC
AGCCAGGACT TCTTACTCTT CAGCCCCGAG GTGGAAAGCC TGCCTGTGGC CAGTAGCTCC
ACGCTGCTCC CGTTGGCCCC CAGCAATTCT ACCAGCCTCA ACTCCACCTT CGGCTCCACC
AACCTGACAG GGCTCTTCTT TCCACCCCAG CTCAATGGCA CAGCCAATGA CACAGCAGGC
CCAGAGCTGC CTGACCCTTT GGGGGGTCTG TTAGATGAAG CTATGTTGGA TGAGATCAGC
CTTATGGACC TGGCCATTGA AGAAGGCTTT AACCCTGTGC AGGCCTCCCA GCTGGAGGAG
GAATTTGACT CTGACTCAGG CCTTTCCTTA GACTCGAGCC ATAGCCCTTC TTCCCTAAGC
AGCTCTGAAG GCAGTTCTTC CTCTTCTTCC TCCTCCTCTT CCTCTTCTTC CTCTGCTTCT
TCCTCTGCCT CTTCCTCCTT TTCTGAGGAA GGTGCGGTTG GCTACAGCTC TGACTCTGAG
ACCCTGGATC TGGAAGAGGC CGAGGGTGCT GTGGGCTACC AGCCTGAGTA TTCCAAGTTC
TGCCGCATGA GCTACCAGGA TCCAGCTCAG CTCTCATGCC TGCCCTACCT GGAGCACGTG
GGCCACAACC ACACATACAA CATGGCACCC AGTGCCCTGG ACTCAGCCGA CCTGCCACCA
CCCAGTGCCC TCAAGAAAGG CAGCAAGGAG AAGCAGGCTG ACTTCCTGGA CAAGCAGATG
AGCCGGGATG AGCACCGAGC CCGAGCCATG AAGATCCCTT TCACCAATGA CAAAATCATC
AACCTGCCTG TGGAGGAGTT CAATGAACTG CTGTCCAAAT ACCAGTTGAG TGAAGCCCAG
CTGAGCCTCA TCCGAGACAT CCGGCGCCGG GGCAAGAACA AGATGGCGGC GCAGAACTGC
CGCAAGCGCA AGCTGGACAC CATCCTGAAT CTGGAGCGTG ATGTGGAGGA CCTGCAGCGT
GACAAAGCCC GGCTGCTGCG GGAGAAAGTG GAGTTCCTGC GCTCCCTGCG ACAGATGAAG
CAGAAGGTCC AGAGCCTGTA CCAGGAGGTG TTTGGGCGGC TGCGAGATGA GAACGGACGA
CCCTACTCGC CCAGTCAGTA TGCGCTCCAG TACGCCGGGG ACGGCAGTGT CCTCCTCATC
CCCCGCACGA TGGCCGACCA GCAGGCCCGG CGGCAGGAGA GGAAGCCAAA GGACCGGAGA
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001317190.1
CDS671..2956
Misc Feature(1)611..613
Misc Feature(2)689..742
Misc Feature(3)2588..2791
Misc Feature(4)2591..2779
Misc Feature(5)2603..2662
Misc Feature(6)order(2615..2620,2624..2632,2636..2653,2657..2665)
Misc Feature(7)order(2660..2662,2669..2674,2681..2686,2690..2695,2702..2707,2711..2716,2723..2728,2732..2737,2744..2749,2753..2758)
Misc Feature(8)2681..2725
Exon (1)1..158
Exon (2)159..1180
Exon (3)1181..1360
Exon (4)1361..1450
Exon (5)1451..1609
Exon (6)1610..4860
Translation

Target ORF information:

RefSeq Version NM_001330261.1
Organism Homo sapiens(Human)
Definition Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001330261.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GATATTGATC TGGGGGCTGG GCGTGAGGTT
TTTGACTATA GTCACCGCCA GAAGGAGCAG GATGTGGAGA AGGAGCTGCG AGATGGAGGC
GAGCAGGACA CCTGGGCAGG CGAGGGCGCG GAAGCTCTGG CACGGAACCT GCTAGTGGAT
GGAGAGACTG GGGAGAGCTT CCCTGCACAG GTGCCTAGTG GGGAGGACCA GACGGCCCTG
TCCCTGGAAG AGTGCCTTAG GCTGCTGGAA GCCACCTGCC CCTTTGGGGA GAATGCTGAG
TTTCCAGCAG ACATTTCCAG CATAACAGAA GCAGTGCCTA GTGAGAGTGA GCCCCCTGCT
CTTCAAAACA ACCTCTTGTC TCCTCTTCTG ACCGGGACAG AGTCACCATT TGATTTGGAA
CAGCAGTGGC AAGATCTCAT GTCCATCATG GAAATGCAGG CCATGGAAGT GAACACATCA
GCAAGTGAAA TCCTGTACAG TGCCCCTCCT GGAGACCCAC TGAGCACCAA CTACAGCCTT
GCCCCCAACA CTCCCATCAA TCAGAATGTC AGCCTGCATC AGGCGTCCCT GGGGGGCTGC
AGCCAGGACT TCTTACTCTT CAGCCCCGAG GTGGAAAGCC TGCCTGTGGC CAGTAGCTCC
ACGCTGCTCC CGTTGGCCCC CAGCAATTCT ACCAGCCTCA ACTCCACCTT CGGCTCCACC
AACCTGACAG GGCTCTTCTT TCCACCCCAG CTCAATGGCA CAGCCAATGA CACAGCAGGC
CCAGAGCTGC CTGACCCTTT GGGGGGTCTG TTAGATGAAG CTATGTTGGA TGAGATCAGC
CTTATGGACC TGGCCATTGA AGAAGGCTTT AACCCTGTGC AGGCCTCCCA GCTGGAGGAG
GAATTTGACT CTGACTCAGG CCTTTCCTTA GACTCGAGCC ATAGCCCTTC TTCCCTAAGC
AGCTCTGAAG GCAGTTCTTC CTCTTCTTCC TCCTCCTCTT CCTCTTCTTC CTCTGCTTCT
TCCTCTGCCT CTTCCTCCTT TTCTGAGGAA GGTGCGGTTG GCTACAGCTC TGACTCTGAG
ACCCTGGATC TGGAAGAGGC CGAGGGTGCT GTGGGCTACC AGCCTGAGTA TTCCAAGTTC
TGCCGCATGA GCTACCAGGA TCCAGCTCAG CTCTCATGCC TGCCCTACCT GGAGCACGTG
GGCCACAACC ACACATACAA CATGGCACCC AGTGCCCTGG ACTCAGCCGA CCTGCCACCA
CCCAGTGCCC TCAAGAAAGG CAGCAAGGAG AAGCAGGCTG ACTTCCTGGA CAAGCAGATG
AGCCGGGATG AGCACCGAGC CCGAGCCATG AAGATCCCTT TCACCAATGA CAAAATCATC
AACCTGCCTG TGGAGGAGTT CAATGAACTG CTGTCCAAAT ACCAGTTGAG TGAAGCCCAG
CTGAGCCTCA TCCGAGACAT CCGGCGCCGG GGCAAGAACA AGATGGCGGC GCAGAACTGC
CGCAAGCGCA AGCTGGACAC CATCCTGAAT CTGGAGCGTG ATGTGGAGGA CCTGCAGCGT
GACAAAGCCC GGCTGCTGCG GGAGAAAGTG GAGTTCCTGC GCTCCCTGCG ACAGATGAAG
CAGAAGGTCC AGAGCCTGTA CCAGGAGGTG TTTGGGCGGC TGCGAGATGA GAACGGACGA
CCCTACTCGC CCAGTCAGTA TGCGCTCCAG TACGCCGGGG ACGGCAGTGT CCTCCTCATC
CCCCGCACGA TGGCCGACCA GCAGGCCCGG CGGCAGGAGA GGAAGCCAAA GGACCGGAGA
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50436
Clone ID Related Accession (Same CDS sequence) NM_001330262.1 , XM_005257412.3
Accession Version NM_001330262.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2229bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-10-09
Organism Homo sapiens(Human)
Product nuclear factor erythroid 2-related factor 1 isoform 3
Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL530578.3, DB097304.1, AC004477.2, L24123.1 and BM971272.1. On Aug 20, 2016 this sequence version replaced XM_005257413.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L24123.1, BC010623.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GACATAGATC TGATTGACAT CCTTTGGCGA
CAGGATATTG ATCTGGGGGC TGGGCGTGAG GTTTTTGACT ATAGTCACCG CCAGAAGGAG
CAGGATGTGG AGAAGGAGCT GCGAGATGGA GGCGAGCAGG ACACCTGGGC AGGCGAGGGC
GCGGAAGCTC TGGCACGGAA CCTGCTAGTG GATGGAGAGA CTGGGGAGAG CTTCCCTGCA
CAGTTTCCAG CAGACATTTC CAGCATAACA GAAGCAGTGC CTAGTGAGAG TGAGCCCCCT
GCTCTTCAAA ACAACCTCTT GTCTCCTCTT CTGACCGGGA CAGAGTCACC ATTTGATTTG
GAACAGCAGT GGCAAGATCT CATGTCCATC ATGGAAATGC AGGCCATGGA AGTGAACACA
TCAGCAAGTG AAATCCTGTA CAGTGCCCCT CCTGGAGACC CACTGAGCAC CAACTACAGC
CTTGCCCCCA ACACTCCCAT CAATCAGAAT GTCAGCCTGC ATCAGGCGTC CCTGGGGGGC
TGCAGCCAGG ACTTCTTACT CTTCAGCCCC GAGGTGGAAA GCCTGCCTGT GGCCAGTAGC
TCCACGCTGC TCCCGTTGGC CCCCAGCAAT TCTACCAGCC TCAACTCCAC CTTCGGCTCC
ACCAACCTGA CAGGGCTCTT CTTTCCACCC CAGCTCAATG GCACAGCCAA TGACACAGCA
GGCCCAGAGC TGCCTGACCC TTTGGGGGGT CTGTTAGATG AAGCTATGTT GGATGAGATC
AGCCTTATGG ACCTGGCCAT TGAAGAAGGC TTTAACCCTG TGCAGGCCTC CCAGCTGGAG
GAGGAATTTG ACTCTGACTC AGGCCTTTCC TTAGACTCGA GCCATAGCCC TTCTTCCCTA
AGCAGCTCTG AAGGCAGTTC TTCCTCTTCT TCCTCCTCCT CTTCCTCTTC TTCCTCTGCT
TCTTCCTCTG CCTCTTCCTC CTTTTCTGAG GAAGGTGCGG TTGGCTACAG CTCTGACTCT
GAGACCCTGG ATCTGGAAGA GGCCGAGGGT GCTGTGGGCT ACCAGCCTGA GTATTCCAAG
TTCTGCCGCA TGAGCTACCA GGATCCAGCT CAGCTCTCAT GCCTGCCCTA CCTGGAGCAC
GTGGGCCACA ACCACACATA CAACATGGCA CCCAGTGCCC TGGACTCAGC CGACCTGCCA
CCACCCAGTG CCCTCAAGAA AGGCAGCAAG GAGAAGCAGG CTGACTTCCT GGACAAGCAG
ATGAGCCGGG ATGAGCACCG AGCCCGAGCC ATGAAGATCC CTTTCACCAA TGACAAAATC
ATCAACCTGC CTGTGGAGGA GTTCAATGAA CTGCTGTCCA AATACCAGTT GAGTGAAGCC
CAGCTGAGCC TCATCCGAGA CATCCGGCGC CGGGGCAAGA ACAAGATGGC GGCGCAGAAC
TGCCGCAAGC GCAAGCTGGA CACCATCCTG AATCTGGAGC GTGATGTGGA GGACCTGCAG
CGTGACAAAG CCCGGCTGCT GCGGGAGAAA GTGGAGTTCC TGCGCTCCCT GCGACAGATG
AAGCAGAAGG TCCAGAGCCT GTACCAGGAG GTGTTTGGGC GGCTGCGAGA TGAGAACGGA
CGACCCTACT CGCCCAGTCA GTATGCGCTC CAGTACGCCG GGGACGGCAG TGTCCTCCTC
ATCCCCCGCA CGATGGCCGA CCAGCAGGCC CGGCGGCAGG AGAGGAAGCC AAAGGACCGG
AGAAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001317191.1
CDS671..2899
Misc Feature(1)611..613
Exon (1)1..158
Exon (2)159..1180
Exon (3)1181..1393
Exon (4)1394..1552
Exon (5)1553..4803
Translation

Target ORF information:

RefSeq Version NM_001330262.1
Organism Homo sapiens(Human)
Definition Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001330262.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GACATAGATC TGATTGACAT CCTTTGGCGA
CAGGATATTG ATCTGGGGGC TGGGCGTGAG GTTTTTGACT ATAGTCACCG CCAGAAGGAG
CAGGATGTGG AGAAGGAGCT GCGAGATGGA GGCGAGCAGG ACACCTGGGC AGGCGAGGGC
GCGGAAGCTC TGGCACGGAA CCTGCTAGTG GATGGAGAGA CTGGGGAGAG CTTCCCTGCA
CAGTTTCCAG CAGACATTTC CAGCATAACA GAAGCAGTGC CTAGTGAGAG TGAGCCCCCT
GCTCTTCAAA ACAACCTCTT GTCTCCTCTT CTGACCGGGA CAGAGTCACC ATTTGATTTG
GAACAGCAGT GGCAAGATCT CATGTCCATC ATGGAAATGC AGGCCATGGA AGTGAACACA
TCAGCAAGTG AAATCCTGTA CAGTGCCCCT CCTGGAGACC CACTGAGCAC CAACTACAGC
CTTGCCCCCA ACACTCCCAT CAATCAGAAT GTCAGCCTGC ATCAGGCGTC CCTGGGGGGC
TGCAGCCAGG ACTTCTTACT CTTCAGCCCC GAGGTGGAAA GCCTGCCTGT GGCCAGTAGC
TCCACGCTGC TCCCGTTGGC CCCCAGCAAT TCTACCAGCC TCAACTCCAC CTTCGGCTCC
ACCAACCTGA CAGGGCTCTT CTTTCCACCC CAGCTCAATG GCACAGCCAA TGACACAGCA
GGCCCAGAGC TGCCTGACCC TTTGGGGGGT CTGTTAGATG AAGCTATGTT GGATGAGATC
AGCCTTATGG ACCTGGCCAT TGAAGAAGGC TTTAACCCTG TGCAGGCCTC CCAGCTGGAG
GAGGAATTTG ACTCTGACTC AGGCCTTTCC TTAGACTCGA GCCATAGCCC TTCTTCCCTA
AGCAGCTCTG AAGGCAGTTC TTCCTCTTCT TCCTCCTCCT CTTCCTCTTC TTCCTCTGCT
TCTTCCTCTG CCTCTTCCTC CTTTTCTGAG GAAGGTGCGG TTGGCTACAG CTCTGACTCT
GAGACCCTGG ATCTGGAAGA GGCCGAGGGT GCTGTGGGCT ACCAGCCTGA GTATTCCAAG
TTCTGCCGCA TGAGCTACCA GGATCCAGCT CAGCTCTCAT GCCTGCCCTA CCTGGAGCAC
GTGGGCCACA ACCACACATA CAACATGGCA CCCAGTGCCC TGGACTCAGC CGACCTGCCA
CCACCCAGTG CCCTCAAGAA AGGCAGCAAG GAGAAGCAGG CTGACTTCCT GGACAAGCAG
ATGAGCCGGG ATGAGCACCG AGCCCGAGCC ATGAAGATCC CTTTCACCAA TGACAAAATC
ATCAACCTGC CTGTGGAGGA GTTCAATGAA CTGCTGTCCA AATACCAGTT GAGTGAAGCC
CAGCTGAGCC TCATCCGAGA CATCCGGCGC CGGGGCAAGA ACAAGATGGC GGCGCAGAAC
TGCCGCAAGC GCAAGCTGGA CACCATCCTG AATCTGGAGC GTGATGTGGA GGACCTGCAG
CGTGACAAAG CCCGGCTGCT GCGGGAGAAA GTGGAGTTCC TGCGCTCCCT GCGACAGATG
AAGCAGAAGG TCCAGAGCCT GTACCAGGAG GTGTTTGGGC GGCTGCGAGA TGAGAACGGA
CGACCCTACT CGCCCAGTCA GTATGCGCTC CAGTACGCCG GGGACGGCAG TGTCCTCCTC
ATCCCCCGCA CGATGGCCGA CCAGCAGGCC CGGCGGCAGG AGAGGAAGCC AAAGGACCGG
AGAAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50435
Clone ID Related Accession (Same CDS sequence) NM_001330261.1 , XM_005257411.3
Accession Version XM_005257411.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2286bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product nuclear factor erythroid 2-related factor 1 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257411.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GATATTGATC TGGGGGCTGG GCGTGAGGTT
TTTGACTATA GTCACCGCCA GAAGGAGCAG GATGTGGAGA AGGAGCTGCG AGATGGAGGC
GAGCAGGACA CCTGGGCAGG CGAGGGCGCG GAAGCTCTGG CACGGAACCT GCTAGTGGAT
GGAGAGACTG GGGAGAGCTT CCCTGCACAG GTGCCTAGTG GGGAGGACCA GACGGCCCTG
TCCCTGGAAG AGTGCCTTAG GCTGCTGGAA GCCACCTGCC CCTTTGGGGA GAATGCTGAG
TTTCCAGCAG ACATTTCCAG CATAACAGAA GCAGTGCCTA GTGAGAGTGA GCCCCCTGCT
CTTCAAAACA ACCTCTTGTC TCCTCTTCTG ACCGGGACAG AGTCACCATT TGATTTGGAA
CAGCAGTGGC AAGATCTCAT GTCCATCATG GAAATGCAGG CCATGGAAGT GAACACATCA
GCAAGTGAAA TCCTGTACAG TGCCCCTCCT GGAGACCCAC TGAGCACCAA CTACAGCCTT
GCCCCCAACA CTCCCATCAA TCAGAATGTC AGCCTGCATC AGGCGTCCCT GGGGGGCTGC
AGCCAGGACT TCTTACTCTT CAGCCCCGAG GTGGAAAGCC TGCCTGTGGC CAGTAGCTCC
ACGCTGCTCC CGTTGGCCCC CAGCAATTCT ACCAGCCTCA ACTCCACCTT CGGCTCCACC
AACCTGACAG GGCTCTTCTT TCCACCCCAG CTCAATGGCA CAGCCAATGA CACAGCAGGC
CCAGAGCTGC CTGACCCTTT GGGGGGTCTG TTAGATGAAG CTATGTTGGA TGAGATCAGC
CTTATGGACC TGGCCATTGA AGAAGGCTTT AACCCTGTGC AGGCCTCCCA GCTGGAGGAG
GAATTTGACT CTGACTCAGG CCTTTCCTTA GACTCGAGCC ATAGCCCTTC TTCCCTAAGC
AGCTCTGAAG GCAGTTCTTC CTCTTCTTCC TCCTCCTCTT CCTCTTCTTC CTCTGCTTCT
TCCTCTGCCT CTTCCTCCTT TTCTGAGGAA GGTGCGGTTG GCTACAGCTC TGACTCTGAG
ACCCTGGATC TGGAAGAGGC CGAGGGTGCT GTGGGCTACC AGCCTGAGTA TTCCAAGTTC
TGCCGCATGA GCTACCAGGA TCCAGCTCAG CTCTCATGCC TGCCCTACCT GGAGCACGTG
GGCCACAACC ACACATACAA CATGGCACCC AGTGCCCTGG ACTCAGCCGA CCTGCCACCA
CCCAGTGCCC TCAAGAAAGG CAGCAAGGAG AAGCAGGCTG ACTTCCTGGA CAAGCAGATG
AGCCGGGATG AGCACCGAGC CCGAGCCATG AAGATCCCTT TCACCAATGA CAAAATCATC
AACCTGCCTG TGGAGGAGTT CAATGAACTG CTGTCCAAAT ACCAGTTGAG TGAAGCCCAG
CTGAGCCTCA TCCGAGACAT CCGGCGCCGG GGCAAGAACA AGATGGCGGC GCAGAACTGC
CGCAAGCGCA AGCTGGACAC CATCCTGAAT CTGGAGCGTG ATGTGGAGGA CCTGCAGCGT
GACAAAGCCC GGCTGCTGCG GGAGAAAGTG GAGTTCCTGC GCTCCCTGCG ACAGATGAAG
CAGAAGGTCC AGAGCCTGTA CCAGGAGGTG TTTGGGCGGC TGCGAGATGA GAACGGACGA
CCCTACTCGC CCAGTCAGTA TGCGCTCCAG TACGCCGGGG ACGGCAGTGT CCTCCTCATC
CCCCGCACGA TGGCCGACCA GCAGGCCCGG CGGCAGGAGA GGAAGCCAAA GGACCGGAGA
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257468.1
CDS598..2883
Misc Feature(1)2515..2718
Misc Feature(2)2518..2706
Misc Feature(3)order(2542..2547,2551..2559,2563..2580,2584..2592)
Misc Feature(4)order(2587..2589,2596..2601,2608..2613,2617..2622,2629..2634,2638..2643,2650..2655,2659..2664,2671..2676,2680..2685)
Translation

Target ORF information:

RefSeq Version XM_005257411.3
Organism Homo sapiens(Human)
Definition Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257411.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GATATTGATC TGGGGGCTGG GCGTGAGGTT
TTTGACTATA GTCACCGCCA GAAGGAGCAG GATGTGGAGA AGGAGCTGCG AGATGGAGGC
GAGCAGGACA CCTGGGCAGG CGAGGGCGCG GAAGCTCTGG CACGGAACCT GCTAGTGGAT
GGAGAGACTG GGGAGAGCTT CCCTGCACAG GTGCCTAGTG GGGAGGACCA GACGGCCCTG
TCCCTGGAAG AGTGCCTTAG GCTGCTGGAA GCCACCTGCC CCTTTGGGGA GAATGCTGAG
TTTCCAGCAG ACATTTCCAG CATAACAGAA GCAGTGCCTA GTGAGAGTGA GCCCCCTGCT
CTTCAAAACA ACCTCTTGTC TCCTCTTCTG ACCGGGACAG AGTCACCATT TGATTTGGAA
CAGCAGTGGC AAGATCTCAT GTCCATCATG GAAATGCAGG CCATGGAAGT GAACACATCA
GCAAGTGAAA TCCTGTACAG TGCCCCTCCT GGAGACCCAC TGAGCACCAA CTACAGCCTT
GCCCCCAACA CTCCCATCAA TCAGAATGTC AGCCTGCATC AGGCGTCCCT GGGGGGCTGC
AGCCAGGACT TCTTACTCTT CAGCCCCGAG GTGGAAAGCC TGCCTGTGGC CAGTAGCTCC
ACGCTGCTCC CGTTGGCCCC CAGCAATTCT ACCAGCCTCA ACTCCACCTT CGGCTCCACC
AACCTGACAG GGCTCTTCTT TCCACCCCAG CTCAATGGCA CAGCCAATGA CACAGCAGGC
CCAGAGCTGC CTGACCCTTT GGGGGGTCTG TTAGATGAAG CTATGTTGGA TGAGATCAGC
CTTATGGACC TGGCCATTGA AGAAGGCTTT AACCCTGTGC AGGCCTCCCA GCTGGAGGAG
GAATTTGACT CTGACTCAGG CCTTTCCTTA GACTCGAGCC ATAGCCCTTC TTCCCTAAGC
AGCTCTGAAG GCAGTTCTTC CTCTTCTTCC TCCTCCTCTT CCTCTTCTTC CTCTGCTTCT
TCCTCTGCCT CTTCCTCCTT TTCTGAGGAA GGTGCGGTTG GCTACAGCTC TGACTCTGAG
ACCCTGGATC TGGAAGAGGC CGAGGGTGCT GTGGGCTACC AGCCTGAGTA TTCCAAGTTC
TGCCGCATGA GCTACCAGGA TCCAGCTCAG CTCTCATGCC TGCCCTACCT GGAGCACGTG
GGCCACAACC ACACATACAA CATGGCACCC AGTGCCCTGG ACTCAGCCGA CCTGCCACCA
CCCAGTGCCC TCAAGAAAGG CAGCAAGGAG AAGCAGGCTG ACTTCCTGGA CAAGCAGATG
AGCCGGGATG AGCACCGAGC CCGAGCCATG AAGATCCCTT TCACCAATGA CAAAATCATC
AACCTGCCTG TGGAGGAGTT CAATGAACTG CTGTCCAAAT ACCAGTTGAG TGAAGCCCAG
CTGAGCCTCA TCCGAGACAT CCGGCGCCGG GGCAAGAACA AGATGGCGGC GCAGAACTGC
CGCAAGCGCA AGCTGGACAC CATCCTGAAT CTGGAGCGTG ATGTGGAGGA CCTGCAGCGT
GACAAAGCCC GGCTGCTGCG GGAGAAAGTG GAGTTCCTGC GCTCCCTGCG ACAGATGAAG
CAGAAGGTCC AGAGCCTGTA CCAGGAGGTG TTTGGGCGGC TGCGAGATGA GAACGGACGA
CCCTACTCGC CCAGTCAGTA TGCGCTCCAG TACGCCGGGG ACGGCAGTGT CCTCCTCATC
CCCCGCACGA TGGCCGACCA GCAGGCCCGG CGGCAGGAGA GGAAGCCAAA GGACCGGAGA
AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50436
Clone ID Related Accession (Same CDS sequence) NM_001330262.1 , XM_005257412.3
Accession Version XM_005257412.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2229bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product nuclear factor erythroid 2-related factor 1 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257412.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GACATAGATC TGATTGACAT CCTTTGGCGA
CAGGATATTG ATCTGGGGGC TGGGCGTGAG GTTTTTGACT ATAGTCACCG CCAGAAGGAG
CAGGATGTGG AGAAGGAGCT GCGAGATGGA GGCGAGCAGG ACACCTGGGC AGGCGAGGGC
GCGGAAGCTC TGGCACGGAA CCTGCTAGTG GATGGAGAGA CTGGGGAGAG CTTCCCTGCA
CAGTTTCCAG CAGACATTTC CAGCATAACA GAAGCAGTGC CTAGTGAGAG TGAGCCCCCT
GCTCTTCAAA ACAACCTCTT GTCTCCTCTT CTGACCGGGA CAGAGTCACC ATTTGATTTG
GAACAGCAGT GGCAAGATCT CATGTCCATC ATGGAAATGC AGGCCATGGA AGTGAACACA
TCAGCAAGTG AAATCCTGTA CAGTGCCCCT CCTGGAGACC CACTGAGCAC CAACTACAGC
CTTGCCCCCA ACACTCCCAT CAATCAGAAT GTCAGCCTGC ATCAGGCGTC CCTGGGGGGC
TGCAGCCAGG ACTTCTTACT CTTCAGCCCC GAGGTGGAAA GCCTGCCTGT GGCCAGTAGC
TCCACGCTGC TCCCGTTGGC CCCCAGCAAT TCTACCAGCC TCAACTCCAC CTTCGGCTCC
ACCAACCTGA CAGGGCTCTT CTTTCCACCC CAGCTCAATG GCACAGCCAA TGACACAGCA
GGCCCAGAGC TGCCTGACCC TTTGGGGGGT CTGTTAGATG AAGCTATGTT GGATGAGATC
AGCCTTATGG ACCTGGCCAT TGAAGAAGGC TTTAACCCTG TGCAGGCCTC CCAGCTGGAG
GAGGAATTTG ACTCTGACTC AGGCCTTTCC TTAGACTCGA GCCATAGCCC TTCTTCCCTA
AGCAGCTCTG AAGGCAGTTC TTCCTCTTCT TCCTCCTCCT CTTCCTCTTC TTCCTCTGCT
TCTTCCTCTG CCTCTTCCTC CTTTTCTGAG GAAGGTGCGG TTGGCTACAG CTCTGACTCT
GAGACCCTGG ATCTGGAAGA GGCCGAGGGT GCTGTGGGCT ACCAGCCTGA GTATTCCAAG
TTCTGCCGCA TGAGCTACCA GGATCCAGCT CAGCTCTCAT GCCTGCCCTA CCTGGAGCAC
GTGGGCCACA ACCACACATA CAACATGGCA CCCAGTGCCC TGGACTCAGC CGACCTGCCA
CCACCCAGTG CCCTCAAGAA AGGCAGCAAG GAGAAGCAGG CTGACTTCCT GGACAAGCAG
ATGAGCCGGG ATGAGCACCG AGCCCGAGCC ATGAAGATCC CTTTCACCAA TGACAAAATC
ATCAACCTGC CTGTGGAGGA GTTCAATGAA CTGCTGTCCA AATACCAGTT GAGTGAAGCC
CAGCTGAGCC TCATCCGAGA CATCCGGCGC CGGGGCAAGA ACAAGATGGC GGCGCAGAAC
TGCCGCAAGC GCAAGCTGGA CACCATCCTG AATCTGGAGC GTGATGTGGA GGACCTGCAG
CGTGACAAAG CCCGGCTGCT GCGGGAGAAA GTGGAGTTCC TGCGCTCCCT GCGACAGATG
AAGCAGAAGG TCCAGAGCCT GTACCAGGAG GTGTTTGGGC GGCTGCGAGA TGAGAACGGA
CGACCCTACT CGCCCAGTCA GTATGCGCTC CAGTACGCCG GGGACGGCAG TGTCCTCCTC
ATCCCCCGCA CGATGGCCGA CCAGCAGGCC CGGCGGCAGG AGAGGAAGCC AAAGGACCGG
AGAAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257469.1
CDS598..2826
Misc Feature(1)2458..2661
Misc Feature(2)2461..2649
Misc Feature(3)order(2485..2490,2494..2502,2506..2523,2527..2535)
Misc Feature(4)order(2530..2532,2539..2544,2551..2556,2560..2565,2572..2577,2581..2586,2593..2598,2602..2607,2614..2619,2623..2628)
Translation

Target ORF information:

RefSeq Version XM_005257412.3
Organism Homo sapiens(Human)
Definition Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257412.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GACATAGATC TGATTGACAT CCTTTGGCGA
CAGGATATTG ATCTGGGGGC TGGGCGTGAG GTTTTTGACT ATAGTCACCG CCAGAAGGAG
CAGGATGTGG AGAAGGAGCT GCGAGATGGA GGCGAGCAGG ACACCTGGGC AGGCGAGGGC
GCGGAAGCTC TGGCACGGAA CCTGCTAGTG GATGGAGAGA CTGGGGAGAG CTTCCCTGCA
CAGTTTCCAG CAGACATTTC CAGCATAACA GAAGCAGTGC CTAGTGAGAG TGAGCCCCCT
GCTCTTCAAA ACAACCTCTT GTCTCCTCTT CTGACCGGGA CAGAGTCACC ATTTGATTTG
GAACAGCAGT GGCAAGATCT CATGTCCATC ATGGAAATGC AGGCCATGGA AGTGAACACA
TCAGCAAGTG AAATCCTGTA CAGTGCCCCT CCTGGAGACC CACTGAGCAC CAACTACAGC
CTTGCCCCCA ACACTCCCAT CAATCAGAAT GTCAGCCTGC ATCAGGCGTC CCTGGGGGGC
TGCAGCCAGG ACTTCTTACT CTTCAGCCCC GAGGTGGAAA GCCTGCCTGT GGCCAGTAGC
TCCACGCTGC TCCCGTTGGC CCCCAGCAAT TCTACCAGCC TCAACTCCAC CTTCGGCTCC
ACCAACCTGA CAGGGCTCTT CTTTCCACCC CAGCTCAATG GCACAGCCAA TGACACAGCA
GGCCCAGAGC TGCCTGACCC TTTGGGGGGT CTGTTAGATG AAGCTATGTT GGATGAGATC
AGCCTTATGG ACCTGGCCAT TGAAGAAGGC TTTAACCCTG TGCAGGCCTC CCAGCTGGAG
GAGGAATTTG ACTCTGACTC AGGCCTTTCC TTAGACTCGA GCCATAGCCC TTCTTCCCTA
AGCAGCTCTG AAGGCAGTTC TTCCTCTTCT TCCTCCTCCT CTTCCTCTTC TTCCTCTGCT
TCTTCCTCTG CCTCTTCCTC CTTTTCTGAG GAAGGTGCGG TTGGCTACAG CTCTGACTCT
GAGACCCTGG ATCTGGAAGA GGCCGAGGGT GCTGTGGGCT ACCAGCCTGA GTATTCCAAG
TTCTGCCGCA TGAGCTACCA GGATCCAGCT CAGCTCTCAT GCCTGCCCTA CCTGGAGCAC
GTGGGCCACA ACCACACATA CAACATGGCA CCCAGTGCCC TGGACTCAGC CGACCTGCCA
CCACCCAGTG CCCTCAAGAA AGGCAGCAAG GAGAAGCAGG CTGACTTCCT GGACAAGCAG
ATGAGCCGGG ATGAGCACCG AGCCCGAGCC ATGAAGATCC CTTTCACCAA TGACAAAATC
ATCAACCTGC CTGTGGAGGA GTTCAATGAA CTGCTGTCCA AATACCAGTT GAGTGAAGCC
CAGCTGAGCC TCATCCGAGA CATCCGGCGC CGGGGCAAGA ACAAGATGGC GGCGCAGAAC
TGCCGCAAGC GCAAGCTGGA CACCATCCTG AATCTGGAGC GTGATGTGGA GGACCTGCAG
CGTGACAAAG CCCGGCTGCT GCGGGAGAAA GTGGAGTTCC TGCGCTCCCT GCGACAGATG
AAGCAGAAGG TCCAGAGCCT GTACCAGGAG GTGTTTGGGC GGCTGCGAGA TGAGAACGGA
CGACCCTACT CGCCCAGTCA GTATGCGCTC CAGTACGCCG GGGACGGCAG TGTCCTCCTC
ATCCCCCGCA CGATGGCCGA CCAGCAGGCC CGGCGGCAGG AGAGGAAGCC AAAGGACCGG
AGAAAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu50437
Clone ID Related Accession (Same CDS sequence) XM_005257415.3
Accession Version XM_005257415.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2196bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product nuclear factor erythroid 2-related factor 1 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257415.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GATATTGATC TGGGGGCTGG GCGTGAGGTT
TTTGACTATA GTCACCGCCA GAAGGAGCAG GATGTGGAGA AGGAGCTGCG AGATGGAGGC
GAGCAGGACA CCTGGGCAGG CGAGGGCGCG GAAGCTCTGG CACGGAACCT GCTAGTGGAT
GGAGAGACTG GGGAGAGCTT CCCTGCACAG TTTCCAGCAG ACATTTCCAG CATAACAGAA
GCAGTGCCTA GTGAGAGTGA GCCCCCTGCT CTTCAAAACA ACCTCTTGTC TCCTCTTCTG
ACCGGGACAG AGTCACCATT TGATTTGGAA CAGCAGTGGC AAGATCTCAT GTCCATCATG
GAAATGCAGG CCATGGAAGT GAACACATCA GCAAGTGAAA TCCTGTACAG TGCCCCTCCT
GGAGACCCAC TGAGCACCAA CTACAGCCTT GCCCCCAACA CTCCCATCAA TCAGAATGTC
AGCCTGCATC AGGCGTCCCT GGGGGGCTGC AGCCAGGACT TCTTACTCTT CAGCCCCGAG
GTGGAAAGCC TGCCTGTGGC CAGTAGCTCC ACGCTGCTCC CGTTGGCCCC CAGCAATTCT
ACCAGCCTCA ACTCCACCTT CGGCTCCACC AACCTGACAG GGCTCTTCTT TCCACCCCAG
CTCAATGGCA CAGCCAATGA CACAGCAGGC CCAGAGCTGC CTGACCCTTT GGGGGGTCTG
TTAGATGAAG CTATGTTGGA TGAGATCAGC CTTATGGACC TGGCCATTGA AGAAGGCTTT
AACCCTGTGC AGGCCTCCCA GCTGGAGGAG GAATTTGACT CTGACTCAGG CCTTTCCTTA
GACTCGAGCC ATAGCCCTTC TTCCCTAAGC AGCTCTGAAG GCAGTTCTTC CTCTTCTTCC
TCCTCCTCTT CCTCTTCTTC CTCTGCTTCT TCCTCTGCCT CTTCCTCCTT TTCTGAGGAA
GGTGCGGTTG GCTACAGCTC TGACTCTGAG ACCCTGGATC TGGAAGAGGC CGAGGGTGCT
GTGGGCTACC AGCCTGAGTA TTCCAAGTTC TGCCGCATGA GCTACCAGGA TCCAGCTCAG
CTCTCATGCC TGCCCTACCT GGAGCACGTG GGCCACAACC ACACATACAA CATGGCACCC
AGTGCCCTGG ACTCAGCCGA CCTGCCACCA CCCAGTGCCC TCAAGAAAGG CAGCAAGGAG
AAGCAGGCTG ACTTCCTGGA CAAGCAGATG AGCCGGGATG AGCACCGAGC CCGAGCCATG
AAGATCCCTT TCACCAATGA CAAAATCATC AACCTGCCTG TGGAGGAGTT CAATGAACTG
CTGTCCAAAT ACCAGTTGAG TGAAGCCCAG CTGAGCCTCA TCCGAGACAT CCGGCGCCGG
GGCAAGAACA AGATGGCGGC GCAGAACTGC CGCAAGCGCA AGCTGGACAC CATCCTGAAT
CTGGAGCGTG ATGTGGAGGA CCTGCAGCGT GACAAAGCCC GGCTGCTGCG GGAGAAAGTG
GAGTTCCTGC GCTCCCTGCG ACAGATGAAG CAGAAGGTCC AGAGCCTGTA CCAGGAGGTG
TTTGGGCGGC TGCGAGATGA GAACGGACGA CCCTACTCGC CCAGTCAGTA TGCGCTCCAG
TACGCCGGGG ACGGCAGTGT CCTCCTCATC CCCCGCACGA TGGCCGACCA GCAGGCCCGG
CGGCAGGAGA GGAAGCCAAA GGACCGGAGA AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005257472.1
CDS598..2793
Misc Feature(1)2425..2628
Misc Feature(2)2428..2616
Misc Feature(3)order(2452..2457,2461..2469,2473..2490,2494..2502)
Misc Feature(4)order(2497..2499,2506..2511,2518..2523,2527..2532,2539..2544,2548..2553,2560..2565,2569..2574,2581..2586,2590..2595)
Translation

Target ORF information:

RefSeq Version XM_005257415.3
Organism Homo sapiens(Human)
Definition Homo sapiens nuclear factor, erythroid 2 like 1 (NFE2L1), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005257415.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
ATGCTTTCTC TGAAGAAATA CTTAACGGAA GGACTTCTCC AGTTCACCAT TCTGCTGAGT 
TTGATTGGGG TACGGGTGGA CGTGGATACT TACCTGACCT CACAGCTTCC CCCACTCCGG
GAGATCATCC TGGGGCCCAG TTCTGCCTAT ACTCAGACCC AGTTCCACAA CCTGAGGAAT
ACCTTGGATG GCTATGGTAT CCACCCCAAG AGCATAGACC TGGACAATTA CTTCACTGCC
CGGCGGCTCC TCAGTCAGGT GAGGGCCCTG GACAGGTTCC AGGTGCCAAC CACTGAGGTA
AATGCCTGGC TGGTTCACCG AGACCCAGAG GGGTCTGTCT CTGGCAGTCA GCCCAACTCA
GGCCTCGCCC TCGAGAGTTC CAGTGGCCTC CAAGATGTGA CAGGCCCAGA CAACGGGGTG
CGAGAAAGCG AAACGGAGCA GGGATTCGGT GAAGATTTGG AGGATTTGGG GGCTGTAGCC
CCCCCAGTCA GTGGAGACTT AACCAAAGAG GATATTGATC TGGGGGCTGG GCGTGAGGTT
TTTGACTATA GTCACCGCCA GAAGGAGCAG GATGTGGAGA AGGAGCTGCG AGATGGAGGC
GAGCAGGACA CCTGGGCAGG CGAGGGCGCG GAAGCTCTGG CACGGAACCT GCTAGTGGAT
GGAGAGACTG GGGAGAGCTT CCCTGCACAG TTTCCAGCAG ACATTTCCAG CATAACAGAA
GCAGTGCCTA GTGAGAGTGA GCCCCCTGCT CTTCAAAACA ACCTCTTGTC TCCTCTTCTG
ACCGGGACAG AGTCACCATT TGATTTGGAA CAGCAGTGGC AAGATCTCAT GTCCATCATG
GAAATGCAGG CCATGGAAGT GAACACATCA GCAAGTGAAA TCCTGTACAG TGCCCCTCCT
GGAGACCCAC TGAGCACCAA CTACAGCCTT GCCCCCAACA CTCCCATCAA TCAGAATGTC
AGCCTGCATC AGGCGTCCCT GGGGGGCTGC AGCCAGGACT TCTTACTCTT CAGCCCCGAG
GTGGAAAGCC TGCCTGTGGC CAGTAGCTCC ACGCTGCTCC CGTTGGCCCC CAGCAATTCT
ACCAGCCTCA ACTCCACCTT CGGCTCCACC AACCTGACAG GGCTCTTCTT TCCACCCCAG
CTCAATGGCA CAGCCAATGA CACAGCAGGC CCAGAGCTGC CTGACCCTTT GGGGGGTCTG
TTAGATGAAG CTATGTTGGA TGAGATCAGC CTTATGGACC TGGCCATTGA AGAAGGCTTT
AACCCTGTGC AGGCCTCCCA GCTGGAGGAG GAATTTGACT CTGACTCAGG CCTTTCCTTA
GACTCGAGCC ATAGCCCTTC TTCCCTAAGC AGCTCTGAAG GCAGTTCTTC CTCTTCTTCC
TCCTCCTCTT CCTCTTCTTC CTCTGCTTCT TCCTCTGCCT CTTCCTCCTT TTCTGAGGAA
GGTGCGGTTG GCTACAGCTC TGACTCTGAG ACCCTGGATC TGGAAGAGGC CGAGGGTGCT
GTGGGCTACC AGCCTGAGTA TTCCAAGTTC TGCCGCATGA GCTACCAGGA TCCAGCTCAG
CTCTCATGCC TGCCCTACCT GGAGCACGTG GGCCACAACC ACACATACAA CATGGCACCC
AGTGCCCTGG ACTCAGCCGA CCTGCCACCA CCCAGTGCCC TCAAGAAAGG CAGCAAGGAG
AAGCAGGCTG ACTTCCTGGA CAAGCAGATG AGCCGGGATG AGCACCGAGC CCGAGCCATG
AAGATCCCTT TCACCAATGA CAAAATCATC AACCTGCCTG TGGAGGAGTT CAATGAACTG
CTGTCCAAAT ACCAGTTGAG TGAAGCCCAG CTGAGCCTCA TCCGAGACAT CCGGCGCCGG
GGCAAGAACA AGATGGCGGC GCAGAACTGC CGCAAGCGCA AGCTGGACAC CATCCTGAAT
CTGGAGCGTG ATGTGGAGGA CCTGCAGCGT GACAAAGCCC GGCTGCTGCG GGAGAAAGTG
GAGTTCCTGC GCTCCCTGCG ACAGATGAAG CAGAAGGTCC AGAGCCTGTA CCAGGAGGTG
TTTGGGCGGC TGCGAGATGA GAACGGACGA CCCTACTCGC CCAGTCAGTA TGCGCTCCAG
TACGCCGGGG ACGGCAGTGT CCTCCTCATC CCCCGCACGA TGGCCGACCA GCAGGCCCGG
CGGCAGGAGA GGAAGCCAAA GGACCGGAGA AAGTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Transcription factor Nrf1 is negatively regulated by its O-GlcNAcylation status.
FEBS letters589(18)2347-58(2015 Aug)
Chen J,Liu X,Lü F,Liu X,Ru Y,Ren Y,Yao L,Zhang Y


book

p97-dependent retrotranslocation and proteolytic processing govern formation of active Nrf1 upon proteasome inhibition.
eLife3e01856(2014)
Radhakrishnan SK,den Besten W,Deshaies RJ


book

The casein kinase 2-nrf1 axis controls the clearance of ubiquitinated proteins by regulating proteasome gene expression.
Molecular and cellular biology33(17)3461-72(2013 Sep)
Tsuchiya Y,Taniguchi H,Ito Y,Morita T,Karim MR,Ohtake N,Fukagai K,Ito T,Okamuro S,Iemura S,Natsume T,Nishida E,Kobayashi A


book

Glycogen synthase kinase 3 regulates expression of nuclear factor-erythroid-2 related transcription factor-1 (Nrf1) and inhibits pro-survival function of Nrf1.
Experimental cell research319(13)1922-31(2013 Aug)
Biswas M,Kwong EK,Park E,Nagra P,Chan JY


book

Human small Maf proteins form heterodimers with CNC family transcription factors and recognize the NF-E2 motif.
Oncogene14(16)1901-10(1997 Apr)
Toki T,Itoh J,Kitazawa J,Arai K,Hatakeyama K,Akasaka J,Igarashi K,Nomura N,Yokoyama M,Yamamoto M,Ito E


 
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