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NOS1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol NOS1
Entrez Gene ID 4842
Full Name nitric oxide synthase 1 (neuronal)
Synonyms IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS
General protein information
Preferred Names
nitric oxide synthase, brain
Names
nitric oxide synthase, brain
NOS type I
neuronal NOS
constitutive NOS
peptidyl-cysteine S-nitrosylase NOS1
NP_000611.1
NP_001191142.1
NP_001191143.1
NP_001191147.1
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

12

12q24.22

Summary The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]. lac of sum
Disorder MIM:

163731


mRNA and Protein(s)

mRNA Protein Name
XM_011538398 XP_011536700 nitric oxide synthase, brain isoform X1
NM_000620 NP_000611 nitric oxide synthase, brain isoform 1
NM_001204213 NP_001191142 nitric oxide synthase, brain isoform 3
NM_001204214 NP_001191143 nitric oxide synthase, brain isoform 3
NM_001204218 NP_001191147 nitric oxide synthase, brain isoform 2



Homo sapiens (human) NOS1 NP_000611.1
Pan troglodytes (chimpanzee) NOS1 XP_003314004.2
Macaca mulatta (Rhesus monkey) NOS1 XP_001083352.2
Canis lupus familiaris (dog) NOS1 NP_001182074.1
Bos taurus (cattle) NOS1 XP_002694631.3
Mus musculus (house mouse) Nos1 NP_032738.1
Rattus norvegicus (Norway rat) Nos1 NP_434686.1
Gallus gallus (chicken) NOS1 XP_004934537.1
Danio rerio (zebrafish) nos1 NP_571735.1
Drosophila melanogaster (fruit fly) Nos NP_523541.2
Xenopus (Silurana) tropicalis (western clawed frog) nos1 XP_004910558.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu21540 XM_011538398 PREDICTED: Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu21020 NM_000620 Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00
OHu21527 NM_001204213 Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu21527 NM_001204214 Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu21540 NM_001204218 Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 25 $727.30
$1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu21540
Clone ID Related Accession (Same CDS sequence) NM_001204218 , XM_011538398
Accession Version XM_011538398.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product nitric oxide synthase, brain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_029419.13) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
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721
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961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC
CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG
AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC
CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011536700.1
CDS572..4978
Misc Feature(1)614..865(+)
Misc Feature(2)650..826(+)
Misc Feature(3)1484..2719(+)
Misc Feature(4)1502..2680(+)
Misc Feature(5)1829..2704(+)
Misc Feature(6)2846..4870(+)
Misc Feature(7)2855..3478(+)
Misc Feature(8)3674..4885(+)
Misc Feature(9)3755..4402(+)
Misc Feature(10)3782..4324(+)
Misc Feature(11)4205..4216(+)
Misc Feature(12)4214..4873(+)
Misc Feature(13)4265..4750(+)
Misc Feature(14)4313..4333(+)
Misc Feature(15)4424..4453(+)
Translation

Target ORF information:

RefSeq Version XM_011538398
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011538398

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC
CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG
AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC
CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21020
Clone ID Related Accession (Same CDS sequence) NM_000620
Accession Version NM_000620.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product nitric oxide synthase, brain isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK307481.1, U17327.1, AK294435.1, BC033208.1, AC026364.36 and BE207961.1. This sequence is a reference standard in the RefSeqGene project. On Feb 18, 2011 this sequence version replaced gi:307938319. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1 (also known as nNOS). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU753241.1, U17327.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA
AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC
AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC
GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG
GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC
AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT
TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC
GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC
CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG
CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC
TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG
CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC
ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA
GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC
GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT
ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT
TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT
TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC
AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC
CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT
TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC
CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG
ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG
CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG
TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT
GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG
GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT
GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC
ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000611.1
CDS712..5016
Misc Feature(1)622..624(+)
Misc Feature(2)712..1326(+)
Misc Feature(3)754..1005(+)
Misc Feature(4)790..966(+)
Misc Feature(5)1198..1446(+)
Misc Feature(6)1624..2859(+)
Misc Feature(7)1642..2820(+)
Misc Feature(8)1969..2844(+)
Misc Feature(9)2899..2961(+)
Misc Feature(10)2947..2949(+)
Misc Feature(11)2974..3033(+)
Misc Feature(12)2986..4908(+)
Misc Feature(13)2995..3516(+)
Misc Feature(14)3265..3267(+)
Misc Feature(15)3265..3267(+)
Misc Feature(16)3265..3267(+)
Misc Feature(17)3265..3267(+)
Misc Feature(18)3265..3267(+)
Misc Feature(19)3712..4923(+)
Misc Feature(20)3793..4440(+)
Misc Feature(21)3820..4362(+)
Misc Feature(22)4243..4254(+)
Misc Feature(23)4252..4911(+)
Misc Feature(24)4303..4788(+)
Misc Feature(25)4351..4371(+)
Misc Feature(26)4462..4491(+)
Misc Feature(27)4960..4962(+)
Exon (1)1..291
Gene:NOS1
Gene Synonym:
Exon (2)292..1436
Gene:NOS1
Gene Synonym:
Exon (3)1437..1563
Gene:NOS1
Gene Synonym:
Exon (4)1564..1692
Gene:NOS1
Gene Synonym:
Exon (5)1693..1838
Gene:NOS1
Gene Synonym:
Exon (6)1839..2001
Gene:NOS1
Gene Synonym:
Exon (7)2002..2093
Gene:NOS1
Gene Synonym:
Exon (8)2094..2235
Gene:NOS1
Gene Synonym:
Exon (9)2236..2375
Gene:NOS1
Gene Synonym:
Exon (10)2376..2550
Gene:NOS1
Gene Synonym:
Exon (11)2551..2652
Gene:NOS1
Gene Synonym:
Exon (12)2653..2847
Gene:NOS1
Gene Synonym:
Exon (13)2848..2933
Gene:NOS1
Gene Synonym:
Exon (14)2934..3078
Gene:NOS1
Gene Synonym:
Exon (15)3079..3183
Gene:NOS1
Gene Synonym:
Exon (16)3184..3242
Gene:NOS1
Gene Synonym:
Exon (17)3243..3359
Gene:NOS1
Gene Synonym:
Exon (18)3360..3534
Gene:NOS1
Gene Synonym:
Exon (19)3535..3673
Gene:NOS1
Gene Synonym:
Exon (20)3674..3752
Gene:NOS1
Gene Synonym:
Exon (21)3753..3946
Gene:NOS1
Gene Synonym:
Exon (22)3947..4116
Gene:NOS1
Gene Synonym:
Exon (23)4117..4327
Gene:NOS1
Gene Synonym:
Exon (24)4328..4415
Gene:NOS1
Gene Synonym:
Exon (25)4416..4537
Gene:NOS1
Gene Synonym:
Exon (26)4538..4686
Gene:NOS1
Gene Synonym:
Exon (27)4687..4881
Gene:NOS1
Gene Synonym:
Exon (28)4882..5000
Gene:NOS1
Gene Synonym:
Exon (29)5001..12183
Gene:NOS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000620
Organism Homo sapiens (human)
Definition Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000620

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
GAAGAAAGGA AGAGCTACAA GGTCCGATTC AACAGCGTCT CCTCCTACTC TGACTCCCAA
AAATCATCAG GCGATGGGCC CGACCTCAGA GACAACTTTG AGAGTGCTGG ACCCCTGGCC
AATGTGAGGT TCTCAGTTTT TGGCCTCGGC TCACGAGCAT ACCCTCACTT TTGCGCCTTC
GGACACGCTG TGGACACCCT CCTGGAAGAA CTGGGAGGGG AGAGGATCCT GAAGATGAGG
GAAGGGGATG AGCTCTGTGG GCAGGAAGAG GCTTTCAGGA CCTGGGCCAA GAAGGTCTTC
AAGGCAGCCT GTGATGTCTT CTGTGTGGGA GATGATGTCA ACATTGAAAA GGCCAACAAT
TCCCTCATCA GCAATGATCG CAGCTGGAAG AGAAACAAGT TCCGCCTCAC CTTTGTGGCC
GAAGCTCCAG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGAGT CTCAGCTGCC
CGGCTCCTTA GCCGTCAAAA CCTCCAGAGC CCTAAATCCA GTCGGTCAAC TATCTTCGTG
CGTCTCCACA CCAACGGGAG CCAGGAGCTG CAGTACCAGC CTGGGGACCA CCTGGGTGTC
TTCCCTGGCA ACCACGAGGA CCTCGTGAAT GCCCTGATCG AGCGGCTGGA GGACGCGCCG
CCTGTCAACC AGATGGTGAA AGTGGAACTG CTGGAGGAGC GGAACACGGC TTTAGGTGTC
ATCAGTAACT GGACAGACGA GCTCCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
TACTACCTGG ACATCACCAC GCCACCAACG CCTCTGCAGC TGCAGCAGTT TGCCTCCCTA
GCTACCAGCG AGAAGGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAGTAC
GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCATCT
ATCCAGATGC CGGCCACCCT GCTCCTGACC CAGCTGTCCC TGCTGCAGCC CCGCTACTAT
TCCATCAGCT CCTCCCCAGA CATGTACCCT GATGAAGTGC ACCTCACTGT GGCCATCGTT
TCCTACCGCA CTCGAGATGG AGAAGGACCA ATTCACCACG GCGTATGCTC CTCCTGGCTC
AACCGGATAC AGGCTGACGA ACTGGTCCCC TGTTTCGTGA GAGGAGCACC CAGCTTCCAC
CTGCCCCGGA ACCCCCAAGT CCCCTGCATC CTCGTTGGAC CAGGCACCGG CATTGCCCCT
TTCCGAAGCT TCTGGCAACA GCGGCAATTT GATATCCAAC ACAAAGGAAT GAACCCCTGC
CCCATGGTCC TGGTCTTCGG GTGCCGGCAA TCCAAGATAG ATCATATCTA CAGGGAAGAG
ACCCTGCAGG CCAAGAACAA GGGGGTCTTC AGAGAGCTGT ACACGGCTTA CTCCCGGGAG
CCAGACAAAC CAAAGAAGTA CGTGCAGGAC ATCCTGCAGG AGCAGCTGGC GGAGTCTGTG
TACCGAGCCC TGAAGGAGCA AGGGGGCCAC ATATACGTCT GTGGGGACGT CACCATGGCT
GCTGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAGCT CTCGGCAGAG
GACGCCGGCG TATTCATCAG CCGGATGAGG GATGACAACC GATACCATGA GGATATTTTT
GGAGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAGTC CATTGCCTTC
ATTGAAGAGA GCAAAAAAGA CACCGATGAG GTTTTCAGCT CCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21527
Clone ID Related Accession (Same CDS sequence) NM_001204213 , NM_001204214
Accession Version NM_001204213.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3297bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product nitric oxide synthase, brain isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068799.14, AK294435.1, U17327.1, AK307481.1, BC033208.1, AC026364.36 and BE207961.1. On Aug 31, 2013 this sequence version replaced gi:530401306. Transcript Variant: This variant (3, also known as TnNOS) contains 2 unique alternate exons (Tex 1 and Tex 2) at the 5' end compared to variant 1, resulting in translation initiation from a downstream AUG, and an isoform (isoform 3, also known as nNOSgamma) with a shorter N-terminus compared to isoform 1. This variant is specifically expressed in the testis, and the encoded isoform has catalytic activity (PMID:9111048). Variants 3 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA962332 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGGCTCCA TCATGCATCC TTCTCAGCAT GCAAGGAGGC CTGAAGACGT CCGCACAAAA 
GGACAGCTCT TCCCTCTCGC CAAAGAGTTT ATTGATCAAT ACTATTCATC AATTAAAAGA
TTTGGCTCCA AAGCCCACAT GGAAAGGCTG GAAGAGGTGA ACAAAGAGAT CGACACCACT
AGCACTTACC AGCTCAAGGA CACAGAGCTC ATCTATGGGG CCAAGCACGC CTGGCGGAAT
GCCTCGCGCT GTGTGGGCAG GATCCAGTGG TCCAAGCTGC AGGTATTCGA TGCCCGTGAC
TGCACCACGG CCCACGGGAT GTTCAACTAC ATCTGTAACC ATGTCAAGTA TGCCACCAAC
AAAGGGAACC TCAGGTCTGC CATCACCATA TTCCCCCAGA GGACAGACGG CAAGCACGAC
TTCCGAGTCT GGAACTCCCA GCTCATCCGC TACGCTGGCT ACAAGCAGCC TGACGGCTCC
ACCCTGGGGG ACCCAGCCAA TGTGCAGTTC ACAGAGATAT GCATACAGCA GGGCTGGAAA
CCGCCTAGAG GCCGCTTCGA TGTCCTGCCG CTCCTGCTTC AGGCCAACGG CAATGACCCT
GAGCTCTTCC AGATTCCTCC AGAGCTGGTG TTGGAAGTTC CCATCAGGCA CCCCAAGTTT
GAGTGGTTCA AGGACCTGGG GCTGAAGTGG TACGGCCTCC CCGCCGTGTC CAACATGCTC
CTAGAGATTG GCGGCCTGGA GTTCAGCGCC TGTCCCTTCA GTGGCTGGTA CATGGGCACA
GAGATTGGTG TCCGCGACTA CTGTGACAAC TCCCGCTACA ATATCCTGGA GGAAGTGGCC
AAGAAGATGA ACTTAGACAT GAGGAAGACG TCCTCCCTGT GGAAGGACCA GGCGCTGGTG
GAGATCAATA TCGCGGTTCT CTATAGCTTC CAGAGTGACA AAGTGACCAT TGTTGACCAT
CACTCCGCCA CCGAGTCCTT CATTAAGCAC ATGGAGAATG AGTACCGCTG CCGGGGGGGC
TGCCCTGCCG ACTGGGTGTG GATCGTGCCC CCCATGTCCG GAAGCATCAC CCCTGTGTTC
CACCAGGAGA TGCTCAACTA CCGGCTCACC CCCTCCTTCG AATACCAGCC TGATCCCTGG
AACACGCATG TCTGGAAAGG CACCAACGGG ACCCCCACAA AGCGGCGAGC CATTGGCTTC
AAGAAGCTAG CAGAAGCTGT CAAGTTCTCG GCCAAGCTGA TGGGGCAGGC TATGGCCAAG
AGGGTGAAAG CGACCATCCT CTATGCCACA GAGACAGGCA AATCGCAAGC TTATGCCAAG
ACCTTGTGTG AGATCTTCAA ACACGCCTTT GATGCCAAGG TGATGTCCAT GGAAGAATAT
GACATTGTGC ACCTGGAACA TGAAACTCTG GTCCTTGTGG TCACCAGCAC CTTTGGCAAT
GGAGATCCCC CTGAGAATGG GGAGAAATTC GGCTGTGCTT TGATGGAAAT GAGGCACCCC
AACTCTGTGC AGGAAGAAAG GAAGAGCTAC AAGGTCCGAT TCAACAGCGT CTCCTCCTAC
TCTGACTCCC AAAAATCATC AGGCGATGGG CCCGACCTCA GAGACAACTT TGAGAGTGCT
GGACCCCTGG CCAATGTGAG GTTCTCAGTT TTTGGCCTCG GCTCACGAGC ATACCCTCAC
TTTTGCGCCT TCGGACACGC TGTGGACACC CTCCTGGAAG AACTGGGAGG GGAGAGGATC
CTGAAGATGA GGGAAGGGGA TGAGCTCTGT GGGCAGGAAG AGGCTTTCAG GACCTGGGCC
AAGAAGGTCT TCAAGGCAGC CTGTGATGTC TTCTGTGTGG GAGATGATGT CAACATTGAA
AAGGCCAACA ATTCCCTCAT CAGCAATGAT CGCAGCTGGA AGAGAAACAA GTTCCGCCTC
ACCTTTGTGG CCGAAGCTCC AGAACTCACA CAAGGTCTAT CCAATGTCCA CAAAAAGCGA
GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA AACCTCCAGA GCCCTAAATC CAGTCGGTCA
ACTATCTTCG TGCGTCTCCA CACCAACGGG AGCCAGGAGC TGCAGTACCA GCCTGGGGAC
CACCTGGGTG TCTTCCCTGG CAACCACGAG GACCTCGTGA ATGCCCTGAT CGAGCGGCTG
GAGGACGCGC CGCCTGTCAA CCAGATGGTG AAAGTGGAAC TGCTGGAGGA GCGGAACACG
GCTTTAGGTG TCATCAGTAA CTGGACAGAC GAGCTCCGCC TCCCGCCCTG CACCATCTTC
CAGGCCTTCA AGTACTACCT GGACATCACC ACGCCACCAA CGCCTCTGCA GCTGCAGCAG
TTTGCCTCCC TAGCTACCAG CGAGAAGGAG AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT
TTGCAGGAGT ACGAGGAATG GAAATGGGGC AAGAACCCCA CCATCGTGGA GGTGCTGGAG
GAGTTCCCAT CTATCCAGAT GCCGGCCACC CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG
CCCCGCTACT ATTCCATCAG CTCCTCCCCA GACATGTACC CTGATGAAGT GCACCTCACT
GTGGCCATCG TTTCCTACCG CACTCGAGAT GGAGAAGGAC CAATTCACCA CGGCGTATGC
TCCTCCTGGC TCAACCGGAT ACAGGCTGAC GAACTGGTCC CCTGTTTCGT GAGAGGAGCA
CCCAGCTTCC ACCTGCCCCG GAACCCCCAA GTCCCCTGCA TCCTCGTTGG ACCAGGCACC
GGCATTGCCC CTTTCCGAAG CTTCTGGCAA CAGCGGCAAT TTGATATCCA ACACAAAGGA
ATGAACCCCT GCCCCATGGT CCTGGTCTTC GGGTGCCGGC AATCCAAGAT AGATCATATC
TACAGGGAAG AGACCCTGCA GGCCAAGAAC AAGGGGGTCT TCAGAGAGCT GTACACGGCT
TACTCCCGGG AGCCAGACAA ACCAAAGAAG TACGTGCAGG ACATCCTGCA GGAGCAGCTG
GCGGAGTCTG TGTACCGAGC CCTGAAGGAG CAAGGGGGCC ACATATACGT CTGTGGGGAC
GTCACCATGG CTGCTGATGT CCTCAAAGCC ATCCAGCGCA TCATGACCCA GCAGGGGAAG
CTCTCGGCAG AGGACGCCGG CGTATTCATC AGCCGGATGA GGGATGACAA CCGATACCAT
GAGGATATTT TTGGAGTCAC CCTGCGAACG TACGAAGTGA CCAACCGCCT TAGATCTGAG
TCCATTGCCT TCATTGAAGA GAGCAAAAAA GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191142.1
CDS312..3608
Misc Feature(1)150..152(+)
Misc Feature(2)312..1451(+)
Misc Feature(3)312..1412(+)
Misc Feature(4)561..1436(+)
Misc Feature(5)1578..3500(+)
Misc Feature(6)1587..2108(+)
Misc Feature(7)2304..3515(+)
Misc Feature(8)2385..3032(+)
Misc Feature(9)2412..2954(+)
Misc Feature(10)2835..2846(+)
Misc Feature(11)2844..3503(+)
Misc Feature(12)2895..3380(+)
Misc Feature(13)2943..2963(+)
Misc Feature(14)3054..3083(+)
Exon (1)1..99
Gene:NOS1
Gene Synonym:
Exon (2)100..155
Gene:NOS1
Gene Synonym:
Exon (3)156..284
Gene:NOS1
Gene Synonym:
Exon (4)285..430
Gene:NOS1
Gene Synonym:
Exon (5)431..593
Gene:NOS1
Gene Synonym:
Exon (6)594..685
Gene:NOS1
Gene Synonym:
Exon (7)686..827
Gene:NOS1
Gene Synonym:
Exon (8)828..967
Gene:NOS1
Gene Synonym:
Exon (9)968..1142
Gene:NOS1
Gene Synonym:
Exon (10)1143..1244
Gene:NOS1
Gene Synonym:
Exon (11)1245..1439
Gene:NOS1
Gene Synonym:
Exon (12)1440..1525
Gene:NOS1
Gene Synonym:
Exon (13)1526..1670
Gene:NOS1
Gene Synonym:
Exon (14)1671..1775
Gene:NOS1
Gene Synonym:
Exon (15)1776..1834
Gene:NOS1
Gene Synonym:
Exon (16)1835..1951
Gene:NOS1
Gene Synonym:
Exon (17)1952..2126
Gene:NOS1
Gene Synonym:
Exon (18)2127..2265
Gene:NOS1
Gene Synonym:
Exon (19)2266..2344
Gene:NOS1
Gene Synonym:
Exon (20)2345..2538
Gene:NOS1
Gene Synonym:
Exon (21)2539..2708
Gene:NOS1
Gene Synonym:
Exon (22)2709..2919
Gene:NOS1
Gene Synonym:
Exon (23)2920..3007
Gene:NOS1
Gene Synonym:
Exon (24)3008..3129
Gene:NOS1
Gene Synonym:
Exon (25)3130..3278
Gene:NOS1
Gene Synonym:
Exon (26)3279..3473
Gene:NOS1
Gene Synonym:
Exon (27)3474..3592
Gene:NOS1
Gene Synonym:
Exon (28)3593..10775
Gene:NOS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204213
Organism Homo sapiens (human)
Definition Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204213

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGGCTCCA TCATGCATCC TTCTCAGCAT GCAAGGAGGC CTGAAGACGT CCGCACAAAA 
GGACAGCTCT TCCCTCTCGC CAAAGAGTTT ATTGATCAAT ACTATTCATC AATTAAAAGA
TTTGGCTCCA AAGCCCACAT GGAAAGGCTG GAAGAGGTGA ACAAAGAGAT CGACACCACT
AGCACTTACC AGCTCAAGGA CACAGAGCTC ATCTATGGGG CCAAGCACGC CTGGCGGAAT
GCCTCGCGCT GTGTGGGCAG GATCCAGTGG TCCAAGCTGC AGGTATTCGA TGCCCGTGAC
TGCACCACGG CCCACGGGAT GTTCAACTAC ATCTGTAACC ATGTCAAGTA TGCCACCAAC
AAAGGGAACC TCAGGTCTGC CATCACCATA TTCCCCCAGA GGACAGACGG CAAGCACGAC
TTCCGAGTCT GGAACTCCCA GCTCATCCGC TACGCTGGCT ACAAGCAGCC TGACGGCTCC
ACCCTGGGGG ACCCAGCCAA TGTGCAGTTC ACAGAGATAT GCATACAGCA GGGCTGGAAA
CCGCCTAGAG GCCGCTTCGA TGTCCTGCCG CTCCTGCTTC AGGCCAACGG CAATGACCCT
GAGCTCTTCC AGATTCCTCC AGAGCTGGTG TTGGAAGTTC CCATCAGGCA CCCCAAGTTT
GAGTGGTTCA AGGACCTGGG GCTGAAGTGG TACGGCCTCC CCGCCGTGTC CAACATGCTC
CTAGAGATTG GCGGCCTGGA GTTCAGCGCC TGTCCCTTCA GTGGCTGGTA CATGGGCACA
GAGATTGGTG TCCGCGACTA CTGTGACAAC TCCCGCTACA ATATCCTGGA GGAAGTGGCC
AAGAAGATGA ACTTAGACAT GAGGAAGACG TCCTCCCTGT GGAAGGACCA GGCGCTGGTG
GAGATCAATA TCGCGGTTCT CTATAGCTTC CAGAGTGACA AAGTGACCAT TGTTGACCAT
CACTCCGCCA CCGAGTCCTT CATTAAGCAC ATGGAGAATG AGTACCGCTG CCGGGGGGGC
TGCCCTGCCG ACTGGGTGTG GATCGTGCCC CCCATGTCCG GAAGCATCAC CCCTGTGTTC
CACCAGGAGA TGCTCAACTA CCGGCTCACC CCCTCCTTCG AATACCAGCC TGATCCCTGG
AACACGCATG TCTGGAAAGG CACCAACGGG ACCCCCACAA AGCGGCGAGC CATTGGCTTC
AAGAAGCTAG CAGAAGCTGT CAAGTTCTCG GCCAAGCTGA TGGGGCAGGC TATGGCCAAG
AGGGTGAAAG CGACCATCCT CTATGCCACA GAGACAGGCA AATCGCAAGC TTATGCCAAG
ACCTTGTGTG AGATCTTCAA ACACGCCTTT GATGCCAAGG TGATGTCCAT GGAAGAATAT
GACATTGTGC ACCTGGAACA TGAAACTCTG GTCCTTGTGG TCACCAGCAC CTTTGGCAAT
GGAGATCCCC CTGAGAATGG GGAGAAATTC GGCTGTGCTT TGATGGAAAT GAGGCACCCC
AACTCTGTGC AGGAAGAAAG GAAGAGCTAC AAGGTCCGAT TCAACAGCGT CTCCTCCTAC
TCTGACTCCC AAAAATCATC AGGCGATGGG CCCGACCTCA GAGACAACTT TGAGAGTGCT
GGACCCCTGG CCAATGTGAG GTTCTCAGTT TTTGGCCTCG GCTCACGAGC ATACCCTCAC
TTTTGCGCCT TCGGACACGC TGTGGACACC CTCCTGGAAG AACTGGGAGG GGAGAGGATC
CTGAAGATGA GGGAAGGGGA TGAGCTCTGT GGGCAGGAAG AGGCTTTCAG GACCTGGGCC
AAGAAGGTCT TCAAGGCAGC CTGTGATGTC TTCTGTGTGG GAGATGATGT CAACATTGAA
AAGGCCAACA ATTCCCTCAT CAGCAATGAT CGCAGCTGGA AGAGAAACAA GTTCCGCCTC
ACCTTTGTGG CCGAAGCTCC AGAACTCACA CAAGGTCTAT CCAATGTCCA CAAAAAGCGA
GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA AACCTCCAGA GCCCTAAATC CAGTCGGTCA
ACTATCTTCG TGCGTCTCCA CACCAACGGG AGCCAGGAGC TGCAGTACCA GCCTGGGGAC
CACCTGGGTG TCTTCCCTGG CAACCACGAG GACCTCGTGA ATGCCCTGAT CGAGCGGCTG
GAGGACGCGC CGCCTGTCAA CCAGATGGTG AAAGTGGAAC TGCTGGAGGA GCGGAACACG
GCTTTAGGTG TCATCAGTAA CTGGACAGAC GAGCTCCGCC TCCCGCCCTG CACCATCTTC
CAGGCCTTCA AGTACTACCT GGACATCACC ACGCCACCAA CGCCTCTGCA GCTGCAGCAG
TTTGCCTCCC TAGCTACCAG CGAGAAGGAG AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT
TTGCAGGAGT ACGAGGAATG GAAATGGGGC AAGAACCCCA CCATCGTGGA GGTGCTGGAG
GAGTTCCCAT CTATCCAGAT GCCGGCCACC CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG
CCCCGCTACT ATTCCATCAG CTCCTCCCCA GACATGTACC CTGATGAAGT GCACCTCACT
GTGGCCATCG TTTCCTACCG CACTCGAGAT GGAGAAGGAC CAATTCACCA CGGCGTATGC
TCCTCCTGGC TCAACCGGAT ACAGGCTGAC GAACTGGTCC CCTGTTTCGT GAGAGGAGCA
CCCAGCTTCC ACCTGCCCCG GAACCCCCAA GTCCCCTGCA TCCTCGTTGG ACCAGGCACC
GGCATTGCCC CTTTCCGAAG CTTCTGGCAA CAGCGGCAAT TTGATATCCA ACACAAAGGA
ATGAACCCCT GCCCCATGGT CCTGGTCTTC GGGTGCCGGC AATCCAAGAT AGATCATATC
TACAGGGAAG AGACCCTGCA GGCCAAGAAC AAGGGGGTCT TCAGAGAGCT GTACACGGCT
TACTCCCGGG AGCCAGACAA ACCAAAGAAG TACGTGCAGG ACATCCTGCA GGAGCAGCTG
GCGGAGTCTG TGTACCGAGC CCTGAAGGAG CAAGGGGGCC ACATATACGT CTGTGGGGAC
GTCACCATGG CTGCTGATGT CCTCAAAGCC ATCCAGCGCA TCATGACCCA GCAGGGGAAG
CTCTCGGCAG AGGACGCCGG CGTATTCATC AGCCGGATGA GGGATGACAA CCGATACCAT
GAGGATATTT TTGGAGTCAC CCTGCGAACG TACGAAGTGA CCAACCGCCT TAGATCTGAG
TCCATTGCCT TCATTGAAGA GAGCAAAAAA GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21527
Clone ID Related Accession (Same CDS sequence) NM_001204213 , NM_001204214
Accession Version NM_001204214.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3297bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product nitric oxide synthase, brain isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068799.14, AK294435.1, U17327.1, AK307481.1, BC033208.1, AC026364.36 and BE207961.1. Transcript Variant: This variant (4, also known as TnNOSb) contains 2 unique alternate exons (Tex 1b and Tex 2) at the 5' end compared to variant 1, resulting in translation initiation from a downstream AUG, and an isoform (3, also known as nNOSgamma) with a shorter N-terminus compared to isoform 1. This variant is specifically expressed in the testis, and the encoded isoform has catalytic activity (PMID:9111048). Variants 3 and 4 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA962332 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGGCTCCA TCATGCATCC TTCTCAGCAT GCAAGGAGGC CTGAAGACGT CCGCACAAAA 
GGACAGCTCT TCCCTCTCGC CAAAGAGTTT ATTGATCAAT ACTATTCATC AATTAAAAGA
TTTGGCTCCA AAGCCCACAT GGAAAGGCTG GAAGAGGTGA ACAAAGAGAT CGACACCACT
AGCACTTACC AGCTCAAGGA CACAGAGCTC ATCTATGGGG CCAAGCACGC CTGGCGGAAT
GCCTCGCGCT GTGTGGGCAG GATCCAGTGG TCCAAGCTGC AGGTATTCGA TGCCCGTGAC
TGCACCACGG CCCACGGGAT GTTCAACTAC ATCTGTAACC ATGTCAAGTA TGCCACCAAC
AAAGGGAACC TCAGGTCTGC CATCACCATA TTCCCCCAGA GGACAGACGG CAAGCACGAC
TTCCGAGTCT GGAACTCCCA GCTCATCCGC TACGCTGGCT ACAAGCAGCC TGACGGCTCC
ACCCTGGGGG ACCCAGCCAA TGTGCAGTTC ACAGAGATAT GCATACAGCA GGGCTGGAAA
CCGCCTAGAG GCCGCTTCGA TGTCCTGCCG CTCCTGCTTC AGGCCAACGG CAATGACCCT
GAGCTCTTCC AGATTCCTCC AGAGCTGGTG TTGGAAGTTC CCATCAGGCA CCCCAAGTTT
GAGTGGTTCA AGGACCTGGG GCTGAAGTGG TACGGCCTCC CCGCCGTGTC CAACATGCTC
CTAGAGATTG GCGGCCTGGA GTTCAGCGCC TGTCCCTTCA GTGGCTGGTA CATGGGCACA
GAGATTGGTG TCCGCGACTA CTGTGACAAC TCCCGCTACA ATATCCTGGA GGAAGTGGCC
AAGAAGATGA ACTTAGACAT GAGGAAGACG TCCTCCCTGT GGAAGGACCA GGCGCTGGTG
GAGATCAATA TCGCGGTTCT CTATAGCTTC CAGAGTGACA AAGTGACCAT TGTTGACCAT
CACTCCGCCA CCGAGTCCTT CATTAAGCAC ATGGAGAATG AGTACCGCTG CCGGGGGGGC
TGCCCTGCCG ACTGGGTGTG GATCGTGCCC CCCATGTCCG GAAGCATCAC CCCTGTGTTC
CACCAGGAGA TGCTCAACTA CCGGCTCACC CCCTCCTTCG AATACCAGCC TGATCCCTGG
AACACGCATG TCTGGAAAGG CACCAACGGG ACCCCCACAA AGCGGCGAGC CATTGGCTTC
AAGAAGCTAG CAGAAGCTGT CAAGTTCTCG GCCAAGCTGA TGGGGCAGGC TATGGCCAAG
AGGGTGAAAG CGACCATCCT CTATGCCACA GAGACAGGCA AATCGCAAGC TTATGCCAAG
ACCTTGTGTG AGATCTTCAA ACACGCCTTT GATGCCAAGG TGATGTCCAT GGAAGAATAT
GACATTGTGC ACCTGGAACA TGAAACTCTG GTCCTTGTGG TCACCAGCAC CTTTGGCAAT
GGAGATCCCC CTGAGAATGG GGAGAAATTC GGCTGTGCTT TGATGGAAAT GAGGCACCCC
AACTCTGTGC AGGAAGAAAG GAAGAGCTAC AAGGTCCGAT TCAACAGCGT CTCCTCCTAC
TCTGACTCCC AAAAATCATC AGGCGATGGG CCCGACCTCA GAGACAACTT TGAGAGTGCT
GGACCCCTGG CCAATGTGAG GTTCTCAGTT TTTGGCCTCG GCTCACGAGC ATACCCTCAC
TTTTGCGCCT TCGGACACGC TGTGGACACC CTCCTGGAAG AACTGGGAGG GGAGAGGATC
CTGAAGATGA GGGAAGGGGA TGAGCTCTGT GGGCAGGAAG AGGCTTTCAG GACCTGGGCC
AAGAAGGTCT TCAAGGCAGC CTGTGATGTC TTCTGTGTGG GAGATGATGT CAACATTGAA
AAGGCCAACA ATTCCCTCAT CAGCAATGAT CGCAGCTGGA AGAGAAACAA GTTCCGCCTC
ACCTTTGTGG CCGAAGCTCC AGAACTCACA CAAGGTCTAT CCAATGTCCA CAAAAAGCGA
GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA AACCTCCAGA GCCCTAAATC CAGTCGGTCA
ACTATCTTCG TGCGTCTCCA CACCAACGGG AGCCAGGAGC TGCAGTACCA GCCTGGGGAC
CACCTGGGTG TCTTCCCTGG CAACCACGAG GACCTCGTGA ATGCCCTGAT CGAGCGGCTG
GAGGACGCGC CGCCTGTCAA CCAGATGGTG AAAGTGGAAC TGCTGGAGGA GCGGAACACG
GCTTTAGGTG TCATCAGTAA CTGGACAGAC GAGCTCCGCC TCCCGCCCTG CACCATCTTC
CAGGCCTTCA AGTACTACCT GGACATCACC ACGCCACCAA CGCCTCTGCA GCTGCAGCAG
TTTGCCTCCC TAGCTACCAG CGAGAAGGAG AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT
TTGCAGGAGT ACGAGGAATG GAAATGGGGC AAGAACCCCA CCATCGTGGA GGTGCTGGAG
GAGTTCCCAT CTATCCAGAT GCCGGCCACC CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG
CCCCGCTACT ATTCCATCAG CTCCTCCCCA GACATGTACC CTGATGAAGT GCACCTCACT
GTGGCCATCG TTTCCTACCG CACTCGAGAT GGAGAAGGAC CAATTCACCA CGGCGTATGC
TCCTCCTGGC TCAACCGGAT ACAGGCTGAC GAACTGGTCC CCTGTTTCGT GAGAGGAGCA
CCCAGCTTCC ACCTGCCCCG GAACCCCCAA GTCCCCTGCA TCCTCGTTGG ACCAGGCACC
GGCATTGCCC CTTTCCGAAG CTTCTGGCAA CAGCGGCAAT TTGATATCCA ACACAAAGGA
ATGAACCCCT GCCCCATGGT CCTGGTCTTC GGGTGCCGGC AATCCAAGAT AGATCATATC
TACAGGGAAG AGACCCTGCA GGCCAAGAAC AAGGGGGTCT TCAGAGAGCT GTACACGGCT
TACTCCCGGG AGCCAGACAA ACCAAAGAAG TACGTGCAGG ACATCCTGCA GGAGCAGCTG
GCGGAGTCTG TGTACCGAGC CCTGAAGGAG CAAGGGGGCC ACATATACGT CTGTGGGGAC
GTCACCATGG CTGCTGATGT CCTCAAAGCC ATCCAGCGCA TCATGACCCA GCAGGGGAAG
CTCTCGGCAG AGGACGCCGG CGTATTCATC AGCCGGATGA GGGATGACAA CCGATACCAT
GAGGATATTT TTGGAGTCAC CCTGCGAACG TACGAAGTGA CCAACCGCCT TAGATCTGAG
TCCATTGCCT TCATTGAAGA GAGCAAAAAA GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191143.1
CDS307..3603
Misc Feature(1)145..147(+)
Misc Feature(2)307..1446(+)
Misc Feature(3)307..1407(+)
Misc Feature(4)556..1431(+)
Misc Feature(5)1573..3495(+)
Misc Feature(6)1582..2103(+)
Misc Feature(7)2299..3510(+)
Misc Feature(8)2380..3027(+)
Misc Feature(9)2407..2949(+)
Misc Feature(10)2830..2841(+)
Misc Feature(11)2839..3498(+)
Misc Feature(12)2890..3375(+)
Misc Feature(13)2938..2958(+)
Misc Feature(14)3049..3078(+)
Exon (1)1..94
Gene:NOS1
Gene Synonym:
Exon (2)95..150
Gene:NOS1
Gene Synonym:
Exon (3)151..279
Gene:NOS1
Gene Synonym:
Exon (4)280..425
Gene:NOS1
Gene Synonym:
Exon (5)426..588
Gene:NOS1
Gene Synonym:
Exon (6)589..680
Gene:NOS1
Gene Synonym:
Exon (7)681..822
Gene:NOS1
Gene Synonym:
Exon (8)823..962
Gene:NOS1
Gene Synonym:
Exon (9)963..1137
Gene:NOS1
Gene Synonym:
Exon (10)1138..1239
Gene:NOS1
Gene Synonym:
Exon (11)1240..1434
Gene:NOS1
Gene Synonym:
Exon (12)1435..1520
Gene:NOS1
Gene Synonym:
Exon (13)1521..1665
Gene:NOS1
Gene Synonym:
Exon (14)1666..1770
Gene:NOS1
Gene Synonym:
Exon (15)1771..1829
Gene:NOS1
Gene Synonym:
Exon (16)1830..1946
Gene:NOS1
Gene Synonym:
Exon (17)1947..2121
Gene:NOS1
Gene Synonym:
Exon (18)2122..2260
Gene:NOS1
Gene Synonym:
Exon (19)2261..2339
Gene:NOS1
Gene Synonym:
Exon (20)2340..2533
Gene:NOS1
Gene Synonym:
Exon (21)2534..2703
Gene:NOS1
Gene Synonym:
Exon (22)2704..2914
Gene:NOS1
Gene Synonym:
Exon (23)2915..3002
Gene:NOS1
Gene Synonym:
Exon (24)3003..3124
Gene:NOS1
Gene Synonym:
Exon (25)3125..3273
Gene:NOS1
Gene Synonym:
Exon (26)3274..3468
Gene:NOS1
Gene Synonym:
Exon (27)3469..3587
Gene:NOS1
Gene Synonym:
Exon (28)3588..10770
Gene:NOS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204214
Organism Homo sapiens (human)
Definition Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204214

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
ATGGGCTCCA TCATGCATCC TTCTCAGCAT GCAAGGAGGC CTGAAGACGT CCGCACAAAA 
GGACAGCTCT TCCCTCTCGC CAAAGAGTTT ATTGATCAAT ACTATTCATC AATTAAAAGA
TTTGGCTCCA AAGCCCACAT GGAAAGGCTG GAAGAGGTGA ACAAAGAGAT CGACACCACT
AGCACTTACC AGCTCAAGGA CACAGAGCTC ATCTATGGGG CCAAGCACGC CTGGCGGAAT
GCCTCGCGCT GTGTGGGCAG GATCCAGTGG TCCAAGCTGC AGGTATTCGA TGCCCGTGAC
TGCACCACGG CCCACGGGAT GTTCAACTAC ATCTGTAACC ATGTCAAGTA TGCCACCAAC
AAAGGGAACC TCAGGTCTGC CATCACCATA TTCCCCCAGA GGACAGACGG CAAGCACGAC
TTCCGAGTCT GGAACTCCCA GCTCATCCGC TACGCTGGCT ACAAGCAGCC TGACGGCTCC
ACCCTGGGGG ACCCAGCCAA TGTGCAGTTC ACAGAGATAT GCATACAGCA GGGCTGGAAA
CCGCCTAGAG GCCGCTTCGA TGTCCTGCCG CTCCTGCTTC AGGCCAACGG CAATGACCCT
GAGCTCTTCC AGATTCCTCC AGAGCTGGTG TTGGAAGTTC CCATCAGGCA CCCCAAGTTT
GAGTGGTTCA AGGACCTGGG GCTGAAGTGG TACGGCCTCC CCGCCGTGTC CAACATGCTC
CTAGAGATTG GCGGCCTGGA GTTCAGCGCC TGTCCCTTCA GTGGCTGGTA CATGGGCACA
GAGATTGGTG TCCGCGACTA CTGTGACAAC TCCCGCTACA ATATCCTGGA GGAAGTGGCC
AAGAAGATGA ACTTAGACAT GAGGAAGACG TCCTCCCTGT GGAAGGACCA GGCGCTGGTG
GAGATCAATA TCGCGGTTCT CTATAGCTTC CAGAGTGACA AAGTGACCAT TGTTGACCAT
CACTCCGCCA CCGAGTCCTT CATTAAGCAC ATGGAGAATG AGTACCGCTG CCGGGGGGGC
TGCCCTGCCG ACTGGGTGTG GATCGTGCCC CCCATGTCCG GAAGCATCAC CCCTGTGTTC
CACCAGGAGA TGCTCAACTA CCGGCTCACC CCCTCCTTCG AATACCAGCC TGATCCCTGG
AACACGCATG TCTGGAAAGG CACCAACGGG ACCCCCACAA AGCGGCGAGC CATTGGCTTC
AAGAAGCTAG CAGAAGCTGT CAAGTTCTCG GCCAAGCTGA TGGGGCAGGC TATGGCCAAG
AGGGTGAAAG CGACCATCCT CTATGCCACA GAGACAGGCA AATCGCAAGC TTATGCCAAG
ACCTTGTGTG AGATCTTCAA ACACGCCTTT GATGCCAAGG TGATGTCCAT GGAAGAATAT
GACATTGTGC ACCTGGAACA TGAAACTCTG GTCCTTGTGG TCACCAGCAC CTTTGGCAAT
GGAGATCCCC CTGAGAATGG GGAGAAATTC GGCTGTGCTT TGATGGAAAT GAGGCACCCC
AACTCTGTGC AGGAAGAAAG GAAGAGCTAC AAGGTCCGAT TCAACAGCGT CTCCTCCTAC
TCTGACTCCC AAAAATCATC AGGCGATGGG CCCGACCTCA GAGACAACTT TGAGAGTGCT
GGACCCCTGG CCAATGTGAG GTTCTCAGTT TTTGGCCTCG GCTCACGAGC ATACCCTCAC
TTTTGCGCCT TCGGACACGC TGTGGACACC CTCCTGGAAG AACTGGGAGG GGAGAGGATC
CTGAAGATGA GGGAAGGGGA TGAGCTCTGT GGGCAGGAAG AGGCTTTCAG GACCTGGGCC
AAGAAGGTCT TCAAGGCAGC CTGTGATGTC TTCTGTGTGG GAGATGATGT CAACATTGAA
AAGGCCAACA ATTCCCTCAT CAGCAATGAT CGCAGCTGGA AGAGAAACAA GTTCCGCCTC
ACCTTTGTGG CCGAAGCTCC AGAACTCACA CAAGGTCTAT CCAATGTCCA CAAAAAGCGA
GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA AACCTCCAGA GCCCTAAATC CAGTCGGTCA
ACTATCTTCG TGCGTCTCCA CACCAACGGG AGCCAGGAGC TGCAGTACCA GCCTGGGGAC
CACCTGGGTG TCTTCCCTGG CAACCACGAG GACCTCGTGA ATGCCCTGAT CGAGCGGCTG
GAGGACGCGC CGCCTGTCAA CCAGATGGTG AAAGTGGAAC TGCTGGAGGA GCGGAACACG
GCTTTAGGTG TCATCAGTAA CTGGACAGAC GAGCTCCGCC TCCCGCCCTG CACCATCTTC
CAGGCCTTCA AGTACTACCT GGACATCACC ACGCCACCAA CGCCTCTGCA GCTGCAGCAG
TTTGCCTCCC TAGCTACCAG CGAGAAGGAG AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT
TTGCAGGAGT ACGAGGAATG GAAATGGGGC AAGAACCCCA CCATCGTGGA GGTGCTGGAG
GAGTTCCCAT CTATCCAGAT GCCGGCCACC CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG
CCCCGCTACT ATTCCATCAG CTCCTCCCCA GACATGTACC CTGATGAAGT GCACCTCACT
GTGGCCATCG TTTCCTACCG CACTCGAGAT GGAGAAGGAC CAATTCACCA CGGCGTATGC
TCCTCCTGGC TCAACCGGAT ACAGGCTGAC GAACTGGTCC CCTGTTTCGT GAGAGGAGCA
CCCAGCTTCC ACCTGCCCCG GAACCCCCAA GTCCCCTGCA TCCTCGTTGG ACCAGGCACC
GGCATTGCCC CTTTCCGAAG CTTCTGGCAA CAGCGGCAAT TTGATATCCA ACACAAAGGA
ATGAACCCCT GCCCCATGGT CCTGGTCTTC GGGTGCCGGC AATCCAAGAT AGATCATATC
TACAGGGAAG AGACCCTGCA GGCCAAGAAC AAGGGGGTCT TCAGAGAGCT GTACACGGCT
TACTCCCGGG AGCCAGACAA ACCAAAGAAG TACGTGCAGG ACATCCTGCA GGAGCAGCTG
GCGGAGTCTG TGTACCGAGC CCTGAAGGAG CAAGGGGGCC ACATATACGT CTGTGGGGAC
GTCACCATGG CTGCTGATGT CCTCAAAGCC ATCCAGCGCA TCATGACCCA GCAGGGGAAG
CTCTCGGCAG AGGACGCCGG CGTATTCATC AGCCGGATGA GGGATGACAA CCGATACCAT
GAGGATATTT TTGGAGTCAC CCTGCGAACG TACGAAGTGA CCAACCGCCT TAGATCTGAG
TCCATTGCCT TCATTGAAGA GAGCAAAAAA GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21540
Clone ID Related Accession (Same CDS sequence) NM_001204218 , XM_011538398
Accession Version NM_001204218.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product nitric oxide synthase, brain isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK307481.1, U17327.1, AK294435.1, AJ004918.1, BC033208.1, AC026364.36 and BE207961.1. Transcript Variant: This variant (2) contains an additional in-frame coding exon compared to variant 1, resulting in a longer isoform (2, also known as nNOSmu) containing a 34 aa protein segment not found in isoform 1. A similar isoform has also been reported in rat and mouse, and the mouse isoform has been shown to have catalytic activity (PMID:9791007). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC
CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG
AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC
CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001191147.1
CDS712..5118
Misc Feature(1)622..624(+)
Misc Feature(2)754..1005(+)
Misc Feature(3)790..966(+)
Misc Feature(4)1624..2859(+)
Misc Feature(5)1642..2820(+)
Misc Feature(6)1969..2844(+)
Misc Feature(7)2986..5010(+)
Misc Feature(8)2995..3618(+)
Misc Feature(9)3814..5025(+)
Misc Feature(10)3895..4542(+)
Misc Feature(11)3922..4464(+)
Misc Feature(12)4345..4356(+)
Misc Feature(13)4354..5013(+)
Misc Feature(14)4405..4890(+)
Misc Feature(15)4453..4473(+)
Misc Feature(16)4564..4593(+)
Exon (1)1..291
Gene:NOS1
Gene Synonym:
Exon (2)292..1436
Gene:NOS1
Gene Synonym:
Exon (3)1437..1563
Gene:NOS1
Gene Synonym:
Exon (4)1564..1692
Gene:NOS1
Gene Synonym:
Exon (5)1693..1838
Gene:NOS1
Gene Synonym:
Exon (6)1839..2001
Gene:NOS1
Gene Synonym:
Exon (7)2002..2093
Gene:NOS1
Gene Synonym:
Exon (8)2094..2235
Gene:NOS1
Gene Synonym:
Exon (9)2236..2375
Gene:NOS1
Gene Synonym:
Exon (10)2376..2550
Gene:NOS1
Gene Synonym:
Exon (11)2551..2652
Gene:NOS1
Gene Synonym:
Exon (12)2653..2847
Gene:NOS1
Gene Synonym:
Exon (13)2848..2933
Gene:NOS1
Gene Synonym:
Exon (14)2934..3078
Gene:NOS1
Gene Synonym:
Exon (15)3079..3183
Gene:NOS1
Gene Synonym:
Exon (16)3184..3242
Gene:NOS1
Gene Synonym:
Exon (17)3243..3344
Gene:NOS1
Gene Synonym:
Exon (18)3345..3461
Gene:NOS1
Gene Synonym:
Exon (19)3462..3636
Gene:NOS1
Gene Synonym:
Exon (20)3637..3775
Gene:NOS1
Gene Synonym:
Exon (21)3776..3854
Gene:NOS1
Gene Synonym:
Exon (22)3855..4048
Gene:NOS1
Gene Synonym:
Exon (23)4049..4218
Gene:NOS1
Gene Synonym:
Exon (24)4219..4429
Gene:NOS1
Gene Synonym:
Exon (25)4430..4517
Gene:NOS1
Gene Synonym:
Exon (26)4518..4639
Gene:NOS1
Gene Synonym:
Exon (27)4640..4788
Gene:NOS1
Gene Synonym:
Exon (28)4789..4983
Gene:NOS1
Gene Synonym:
Exon (29)4984..5102
Gene:NOS1
Gene Synonym:
Exon (30)5103..12285
Gene:NOS1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001204218
Organism Homo sapiens (human)
Definition Homo sapiens nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001204218

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
ATGGAGGATC ACATGTTCGG TGTTCAGCAA ATCCAGCCCA ATGTCATTTC TGTTCGTCTC 
TTCAAGCGCA AAGTTGGGGG CCTGGGATTT CTGGTGAAGG AGCGGGTCAG TAAGCCGCCC
GTGATCATCT CTGACCTGAT TCGTGGGGGC GCCGCAGAGC AGAGTGGCCT CATCCAGGCC
GGAGACATCA TTCTTGCGGT CAACGGCCGG CCCTTGGTGG ACCTGAGCTA TGACAGCGCC
CTGGAGGTAC TCAGAGGCAT TGCCTCTGAG ACCCACGTGG TCCTCATTCT GAGGGGCCCT
GAAGGTTTCA CCACGCACCT GGAGACCACC TTTACAGGTG ATGGGACCCC CAAGACCATC
CGGGTGACAC AGCCCCTGGG TCCCCCCACC AAAGCCGTGG ATCTGTCCCA CCAGCCACCG
GCCGGCAAAG AACAGCCCCT GGCAGTGGAT GGGGCCTCGG GTCCCGGGAA TGGGCCTCAG
CATGCCTACG ATGATGGGCA GGAGGCTGGC TCACTCCCCC ATGCCAACGG CCTGGCCCCC
AGGCCCCCAG GCCAGGACCC CGCGAAGAAA GCAACCAGAG TCAGCCTCCA AGGCAGAGGG
GAGAACAATG AACTGCTCAA GGAGATAGAG CCTGTGCTGA GCCTTCTCAC CAGTGGGAGC
AGAGGGGTCA AGGGAGGGGC ACCTGCCAAG GCAGAGATGA AAGATATGGG AATCCAGGTG
GACAGAGATT TGGACGGCAA GTCACACAAA CCTCTGCCCC TCGGCGTGGA GAACGACCGA
GTCTTCAATG ACCTATGGGG GAAGGGCAAT GTGCCTGTCG TCCTCAACAA CCCATATTCA
GAGAAGGAGC AGCCCCCCAC CTCAGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
AAGTGTCCAC GCTTCCTCAA GGTCAAGAAC TGGGAGACTG AGGTGGTTCT CACTGACACC
CTCCACCTTA AGAGCACATT GGAAACGGGA TGCACTGAGT ACATCTGCAT GGGCTCCATC
ATGCATCCTT CTCAGCATGC AAGGAGGCCT GAAGACGTCC GCACAAAAGG ACAGCTCTTC
CCTCTCGCCA AAGAGTTTAT TGATCAATAC TATTCATCAA TTAAAAGATT TGGCTCCAAA
GCCCACATGG AAAGGCTGGA AGAGGTGAAC AAAGAGATCG ACACCACTAG CACTTACCAG
CTCAAGGACA CAGAGCTCAT CTATGGGGCC AAGCACGCCT GGCGGAATGC CTCGCGCTGT
GTGGGCAGGA TCCAGTGGTC CAAGCTGCAG GTATTCGATG CCCGTGACTG CACCACGGCC
CACGGGATGT TCAACTACAT CTGTAACCAT GTCAAGTATG CCACCAACAA AGGGAACCTC
AGGTCTGCCA TCACCATATT CCCCCAGAGG ACAGACGGCA AGCACGACTT CCGAGTCTGG
AACTCCCAGC TCATCCGCTA CGCTGGCTAC AAGCAGCCTG ACGGCTCCAC CCTGGGGGAC
CCAGCCAATG TGCAGTTCAC AGAGATATGC ATACAGCAGG GCTGGAAACC GCCTAGAGGC
CGCTTCGATG TCCTGCCGCT CCTGCTTCAG GCCAACGGCA ATGACCCTGA GCTCTTCCAG
ATTCCTCCAG AGCTGGTGTT GGAAGTTCCC ATCAGGCACC CCAAGTTTGA GTGGTTCAAG
GACCTGGGGC TGAAGTGGTA CGGCCTCCCC GCCGTGTCCA ACATGCTCCT AGAGATTGGC
GGCCTGGAGT TCAGCGCCTG TCCCTTCAGT GGCTGGTACA TGGGCACAGA GATTGGTGTC
CGCGACTACT GTGACAACTC CCGCTACAAT ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
TTAGACATGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAATATC
GCGGTTCTCT ATAGCTTCCA GAGTGACAAA GTGACCATTG TTGACCATCA CTCCGCCACC
GAGTCCTTCA TTAAGCACAT GGAGAATGAG TACCGCTGCC GGGGGGGCTG CCCTGCCGAC
TGGGTGTGGA TCGTGCCCCC CATGTCCGGA AGCATCACCC CTGTGTTCCA CCAGGAGATG
CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCCTGGAA CACGCATGTC
TGGAAAGGCA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TTGGCTTCAA GAAGCTAGCA
GAAGCTGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCTA TGGCCAAGAG GGTGAAAGCG
ACCATCCTCT ATGCCACAGA GACAGGCAAA TCGCAAGCTT ATGCCAAGAC CTTGTGTGAG
ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
CTGGAACATG AAACTCTGGT CCTTGTGGTC ACCAGCACCT TTGGCAATGG AGATCCCCCT
GAGAATGGGG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GGCACCCCAA CTCTGTGCAG
GAAGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
AATGGTGACA CAGAAGTCCA CGGTCTGGCT GCAGCCCGTG ACAGCCAGCA CAGGAGCTAC
AAGGTCCGAT TCAACAGCGT CTCCTCCTAC TCTGACTCCC AAAAATCATC AGGCGATGGG
CCCGACCTCA GAGACAACTT TGAGAGTGCT GGACCCCTGG CCAATGTGAG GTTCTCAGTT
TTTGGCCTCG GCTCACGAGC ATACCCTCAC TTTTGCGCCT TCGGACACGC TGTGGACACC
CTCCTGGAAG AACTGGGAGG GGAGAGGATC CTGAAGATGA GGGAAGGGGA TGAGCTCTGT
GGGCAGGAAG AGGCTTTCAG GACCTGGGCC AAGAAGGTCT TCAAGGCAGC CTGTGATGTC
TTCTGTGTGG GAGATGATGT CAACATTGAA AAGGCCAACA ATTCCCTCAT CAGCAATGAT
CGCAGCTGGA AGAGAAACAA GTTCCGCCTC ACCTTTGTGG CCGAAGCTCC AGAACTCACA
CAAGGTCTAT CCAATGTCCA CAAAAAGCGA GTCTCAGCTG CCCGGCTCCT TAGCCGTCAA
AACCTCCAGA GCCCTAAATC CAGTCGGTCA ACTATCTTCG TGCGTCTCCA CACCAACGGG
AGCCAGGAGC TGCAGTACCA GCCTGGGGAC CACCTGGGTG TCTTCCCTGG CAACCACGAG
GACCTCGTGA ATGCCCTGAT CGAGCGGCTG GAGGACGCGC CGCCTGTCAA CCAGATGGTG
AAAGTGGAAC TGCTGGAGGA GCGGAACACG GCTTTAGGTG TCATCAGTAA CTGGACAGAC
GAGCTCCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGACATCACC
ACGCCACCAA CGCCTCTGCA GCTGCAGCAG TTTGCCTCCC TAGCTACCAG CGAGAAGGAG
AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAGT ACGAGGAATG GAAATGGGGC
AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCAT CTATCCAGAT GCCGGCCACC
CTGCTCCTGA CCCAGCTGTC CCTGCTGCAG CCCCGCTACT ATTCCATCAG CTCCTCCCCA
GACATGTACC CTGATGAAGT GCACCTCACT GTGGCCATCG TTTCCTACCG CACTCGAGAT
GGAGAAGGAC CAATTCACCA CGGCGTATGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
GAACTGGTCC CCTGTTTCGT GAGAGGAGCA CCCAGCTTCC ACCTGCCCCG GAACCCCCAA
GTCCCCTGCA TCCTCGTTGG ACCAGGCACC GGCATTGCCC CTTTCCGAAG CTTCTGGCAA
CAGCGGCAAT TTGATATCCA ACACAAAGGA ATGAACCCCT GCCCCATGGT CCTGGTCTTC
GGGTGCCGGC AATCCAAGAT AGATCATATC TACAGGGAAG AGACCCTGCA GGCCAAGAAC
AAGGGGGTCT TCAGAGAGCT GTACACGGCT TACTCCCGGG AGCCAGACAA ACCAAAGAAG
TACGTGCAGG ACATCCTGCA GGAGCAGCTG GCGGAGTCTG TGTACCGAGC CCTGAAGGAG
CAAGGGGGCC ACATATACGT CTGTGGGGAC GTCACCATGG CTGCTGATGT CCTCAAAGCC
ATCCAGCGCA TCATGACCCA GCAGGGGAAG CTCTCGGCAG AGGACGCCGG CGTATTCATC
AGCCGGATGA GGGATGACAA CCGATACCAT GAGGATATTT TTGGAGTCAC CCTGCGAACG
TACGAAGTGA CCAACCGCCT TAGATCTGAG TCCATTGCCT TCATTGAAGA GAGCAAAAAA
GACACCGATG AGGTTTTCAG CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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