NOTCH2 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following NOTCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOTCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16899 NM_001200001.1
Latest version!
Homo sapiens notch receptor 2 (NOTCH2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu19515 NM_024408.4
Latest version!
Homo sapiens notch receptor 2 (NOTCH2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu33467 XM_011541520.2
Latest version!
Homo sapiens notch 2 (NOTCH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24 $1301.30
$1859.00
OHu33467 XM_005270901.3
Latest version!
Homo sapiens notch 2 (NOTCH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24 $1301.30
$1859.00
OHu33467 XM_017001373.1
Latest version!
Homo sapiens notch 2 (NOTCH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24 $1301.30
$1859.00
OHu90064 XM_017001372.1
Latest version!
Homo sapiens notch 2 (NOTCH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu59298 XM_011541519.2
Latest version!
Homo sapiens notch 2 (NOTCH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
View Old Accession Versions >>
OHu19515 NM_024408.3 Homo sapiens notch 2 (NOTCH2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu16899
    Clone ID Related Accession (Same CDS sequence) NM_001200001.1
    Accession Version NM_001200001.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-07
    Organism Homo sapiens(human)
    Product neurogenic locus notch homolog protein 2 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB290699.1, BC071562.1 and AF315356.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071562.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCGCCC TGCGCCCCGC TCTGCTGTGG GCGCTGCTGG CGCTCTGGCT GTGCTGCGCG 
    GCCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG
    GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC
    TGCCAGTACT CAACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA
    TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG
    GAGTGCCAAT GGACGGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG
    ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC
    CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC
    CTGTATGTGC CCTGTGCACC CTCACCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT
    GACTTCACTT TTGAGTGCAA CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT
    ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC
    ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA
    GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT
    TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTGCC
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC
    CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC
    CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT
    CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG
    TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC
    CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG
    GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT
    CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGGGTT
    AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AATCTGTATG
    GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT
    GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG
    GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA
    TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA
    TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC
    TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC
    CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG
    TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG
    CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA
    CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTAT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT
    TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT
    GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTGAATGA GGGAACGTGT
    GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT
    CAGACCCTGG TGAATCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG
    AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG
    CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC
    CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC
    TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC
    GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGCG AGTGTGTCCC AGGCTATCAG
    GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC
    ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGTATG
    AAATCATCCT TATCCATTTT CCATCCAGGG CATTGTCTTA AGTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186930.1
    CDS298..4005
    Translation

    Target ORF information:

    RefSeq Version NM_001200001.1
    Organism Homo sapiens(human)
    Definition Homo sapiens notch receptor 2 (NOTCH2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001200001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCGCCC TGCGCCCCGC TCTGCTGTGG GCGCTGCTGG CGCTCTGGCT GTGCTGCGCG 
    GCCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG
    GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC
    TGCCAGTACT CAACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA
    TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG
    GAGTGCCAAT GGACGGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG
    ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC
    CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC
    CTGTATGTGC CCTGTGCACC CTCACCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT
    GACTTCACTT TTGAGTGCAA CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT
    ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC
    ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA
    GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT
    TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTGCC
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC
    CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC
    CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT
    CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG
    TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC
    CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG
    GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT
    CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGGGTT
    AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AATCTGTATG
    GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT
    GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG
    GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA
    TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA
    TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC
    TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC
    CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG
    TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG
    CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA
    CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTAT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT
    TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT
    GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTGAATGA GGGAACGTGT
    GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT
    CAGACCCTGG TGAATCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG
    AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG
    CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC
    CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC
    TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC
    GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGCG AGTGTGTCCC AGGCTATCAG
    GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC
    ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGTATG
    AAATCATCCT TATCCATTTT CCATCCAGGG CATTGTCTTA AGTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19515
    Clone ID Related Accession (Same CDS sequence) NM_024408.3 , NM_024408.4
    Accession Version NM_024408.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-10
    Organism Homo sapiens(human)
    Product neurogenic locus notch homolog protein 2 isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB290699.1, BC071562.1, AF315356.1 and AL133036.1. This sequence is a reference standard in the RefSeqGene project. On Jan 6, 2011 this sequence version replaced NM_024408.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCCCGCCC TGCGCCCCGC TCTGCTGTGG GCGCTGCTGG CGCTCTGGCT GTGCTGCGCG 
    GCCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG
    GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC
    TGCCAGTACT CAACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA
    TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG
    GAGTGCCAAT GGACGGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG
    ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC
    CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC
    CTGTATGTGC CCTGTGCACC CTCACCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT
    GACTTCACTT TTGAGTGCAA CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT
    ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC
    ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA
    GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT
    TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTGCC
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC
    CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC
    CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT
    CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG
    TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC
    CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG
    GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT
    CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGGGTT
    AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AATCTGTATG
    GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT
    GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG
    GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA
    TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA
    TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC
    TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC
    CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG
    TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG
    CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA
    CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTAT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT
    TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT
    GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTGAATGA GGGAACGTGT
    GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT
    CAGACCCTGG TGAATCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG
    AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG
    CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC
    CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC
    TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC
    GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGCG AGTGTGTCCC AGGCTATCAG
    GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC
    ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGCCTA
    CTCTGTGAAG AGAACATTGA TGACTGTGCC CGGGGTCCCC ATTGCCTTAA TGGTGGTCAG
    TGCATGGATA GGATTGGAGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC TGGGGAGCGT
    TGTGAGGGAG ACATCAACGA GTGCCTCTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGTATACAGC TCACCAATGA CTACCTGTGT GTTTGCCGTA GTGCCTTTAC TGGCCGGCAC
    TGTGAAACCT TCGTCGATGT GTGTCCCCAG ATGCCCTGCC TGAATGGAGG GACTTGTGCT
    GTGGCCAGTA ACATGCCTGA TGGTTTCATT TGCCGTTGTC CCCCGGGATT TTCCGGGGCA
    AGGTGCCAGA GCAGCTGTGG ACAAGTGAAA TGTAGGAAGG GGGAGCAGTG TGTGCACACC
    GCCTCTGGAC CCCGCTGCTT CTGCCCCAGT CCCCGGGACT GCGAGTCAGG CTGTGCCAGT
    AGCCCCTGCC AGCACGGGGG CAGCTGCCAC CCTCAGCGCC AGCCTCCTTA TTACTCCTGC
    CAGTGTGCCC CACCATTCTC GGGTAGCCGC TGTGAACTCT ACACGGCACC CCCCAGCACC
    CCTCCTGCCA CCTGTCTGAG CCAGTATTGT GCCGACAAAG CTCGGGATGG CGTCTGTGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCTCT CACCATGGAG
    AACCCCTGGG CCAACTGCTC CTCCCCACTT CCCTGCTGGG ATTATATCAA CAACCAGTGT
    GATGAGCTGT GCAACACGGT CGAGTGCCTG TTTGACAACT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGGTGCAACA GTGAGGAGTG TGGTTGGGAT GGGCTGGACT GTGCTGCTGA CCAACCTGAG
    AACCTGGCAG AAGGTACCCT GGTTATTGTG GTATTGATGC CACCTGAACA ACTGCTCCAG
    GATGCTCGCA GCTTCTTGCG GGCACTGGGT ACCCTGCTCC ACACCAACCT GCGCATTAAG
    CGGGACTCCC AGGGGGAACT CATGGTGTAC CCCTATTATG GTGAGAAGTC AGCTGCTATG
    AAGAAACAGA GGATGACACG CAGATCCCTT CCTGGTGAAC AAGAACAGGA GGTGGCTGGC
    TCTAAAGTCT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCACTGCTTC
    AAGAACACGG ATGCAGCAGC AGCTCTCCTG GCCTCTCACG CCATACAGGG GACCCTGTCA
    TACCCTCTTG TGTCTGTCGT CAGTGAATCC CTGACTCCAG AACGCACTCA GCTCCTCTAT
    CTCCTTGCTG TTGCTGTTGT CATCATTCTG TTTATTATTC TGCTGGGGGT AATCATGGCA
    AAACGAAAGC GTAAGCATGG CTCTCTCTGG CTGCCTGAAG GTTTCACTCT TCGCCGAGAT
    GCAAGCAATC ACAAGCGTCG TGAGCCAGTG GGACAGGATG CTGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAAGC TAACCTAATT GGTACTGGAA CAAGTGAACA CTGGGTCGAT
    GATGAAGGGC CCCAGCCAAA GAAAGTAAAG GCTGAAGATG AGGCCTTACT CTCAGAAGAA
    GATGACCCCA TTGATCGACG GCCATGGACA CAGCAGCACC TTGAAGCTGC AGACATCCGT
    AGGACACCAT CGCTGGCTCT CACCCCTCCT CAGGCAGAGC AGGAGGTGGA TGTGTTAGAT
    GTGAATGTCC GTGGCCCAGA TGGCTGCACC CCATTGATGT TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATT TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCTAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTCCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCACGGGCT GATGCTGCCA AGCGTCTCCT GGATGCAGGT
    GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGTCCAC TCCATGCTGC AGTGGCAGCT
    GATGCCCAAG GTGTCTTCCA GATTCTGATT CGCAACCGAG TAACTGATCT AGATGCCAGG
    ATGAATGATG GTACTACACC CCTGATCCTG GCTGCCCGCC TGGCTGTGGA GGGAATGGTG
    GCAGAACTGA TCAACTGCCA AGCGGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAATGTG GAGGCAACTC TTTTGTTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAGGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG
    GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGAGA CATCACAGAC
    CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGATCGCA TGCACCATGA CATTGTGCGC
    CTTCTGGATG AATACAATGT GACCCCAAGC CCTCCAGGCA CCGTGTTGAC TTCTGCTCTC
    TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TGAAGCACAC CCCAATGGGC
    AAGAAGTCTA GACGGCCCAG TGCCAAGAGT ACCATGCCTA CTAGCCTCCC TAACCTTGCC
    AAGGAGGCAA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA GAAGGTCCAA
    CTGTCTGAGA GTTCAGTAAC TTTATCCCCT GTTGATTCCC TAGAATCTCC TCACACGTAT
    GTTTCCGACA CCACATCCTC TCCAATGATT ACATCCCCTG GGATCTTACA GGCCTCACCC
    AACCCTATGT TGGCCACTGC CGCCCCTCCT GCCCCAGTCC ATGCCCAGCA TGCACTATCT
    TTTTCTAACC TTCATGAAAT GCAGCCTTTG GCACATGGGG CCAGCACTGT GCTTCCCTCA
    GTGAGCCAGT TGCTATCCCA CCACCACATT GTGTCTCCAG GCAGTGGCAG TGCTGGAAGC
    TTGAGTAGGC TCCATCCAGT CCCAGTCCCA GCAGATTGGA TGAACCGCAT GGAGGTGAAT
    GAGACCCAGT ACAATGAGAT GTTTGGTATG GTCCTGGCTC CAGCTGAGGG CACCCATCCT
    GGCATAGCTC CCCAGAGCAG GCCACCTGAA GGGAAGCACA TAACCACCCC TCGGGAGCCC
    TTGCCCCCCA TTGTGACTTT CCAGCTCATC CCTAAAGGCA GTATTGCCCA ACCAGCGGGG
    GCTCCCCAGC CTCAGTCCAC CTGCCCTCCA GCTGTTGCGG GCCCCCTGCC CACCATGTAC
    CAGATTCCAG AAATGGCCCG TTTGCCCAGT GTGGCTTTCC CCACTGCCAT GATGCCCCAG
    CAGGACGGGC AGGTAGCTCA GACCATTCTC CCAGCCTATC ATCCTTTCCC AGCCTCTGTG
    GGCAAGTACC CCACACCCCC TTCACAGCAC AGTTATGCTT CCTCAAATGC TGCTGAGCGA
    ACACCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG
    TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC
    ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT
    GAGCCACCAC ACAACAACAT GCAGGTTTAT GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_077719.2
    CDS298..7713
    Translation

    Target ORF information:

    RefSeq Version NM_024408.3
    Organism Homo sapiens(human)
    Definition Homo sapiens notch 2 (NOTCH2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_024408.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCCCGCCC TGCGCCCCGC TCTGCTGTGG GCGCTGCTGG CGCTCTGGCT GTGCTGCGCG 
    GCCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG
    GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC
    TGCCAGTACT CAACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA
    TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG
    GAGTGCCAAT GGACGGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG
    ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC
    CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC
    CTGTATGTGC CCTGTGCACC CTCACCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT
    GACTTCACTT TTGAGTGCAA CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT
    ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC
    ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA
    GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT
    TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTGCC
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC
    CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC
    CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT
    CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG
    TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC
    CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG
    GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT
    CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGGGTT
    AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AATCTGTATG
    GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT
    GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG
    GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA
    TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA
    TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC
    TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC
    CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG
    TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG
    CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA
    CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTAT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT
    TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT
    GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTGAATGA GGGAACGTGT
    GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT
    CAGACCCTGG TGAATCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG
    AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG
    CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC
    CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC
    TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC
    GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGCG AGTGTGTCCC AGGCTATCAG
    GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC
    ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGCCTA
    CTCTGTGAAG AGAACATTGA TGACTGTGCC CGGGGTCCCC ATTGCCTTAA TGGTGGTCAG
    TGCATGGATA GGATTGGAGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC TGGGGAGCGT
    TGTGAGGGAG ACATCAACGA GTGCCTCTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGTATACAGC TCACCAATGA CTACCTGTGT GTTTGCCGTA GTGCCTTTAC TGGCCGGCAC
    TGTGAAACCT TCGTCGATGT GTGTCCCCAG ATGCCCTGCC TGAATGGAGG GACTTGTGCT
    GTGGCCAGTA ACATGCCTGA TGGTTTCATT TGCCGTTGTC CCCCGGGATT TTCCGGGGCA
    AGGTGCCAGA GCAGCTGTGG ACAAGTGAAA TGTAGGAAGG GGGAGCAGTG TGTGCACACC
    GCCTCTGGAC CCCGCTGCTT CTGCCCCAGT CCCCGGGACT GCGAGTCAGG CTGTGCCAGT
    AGCCCCTGCC AGCACGGGGG CAGCTGCCAC CCTCAGCGCC AGCCTCCTTA TTACTCCTGC
    CAGTGTGCCC CACCATTCTC GGGTAGCCGC TGTGAACTCT ACACGGCACC CCCCAGCACC
    CCTCCTGCCA CCTGTCTGAG CCAGTATTGT GCCGACAAAG CTCGGGATGG CGTCTGTGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCTCT CACCATGGAG
    AACCCCTGGG CCAACTGCTC CTCCCCACTT CCCTGCTGGG ATTATATCAA CAACCAGTGT
    GATGAGCTGT GCAACACGGT CGAGTGCCTG TTTGACAACT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGGTGCAACA GTGAGGAGTG TGGTTGGGAT GGGCTGGACT GTGCTGCTGA CCAACCTGAG
    AACCTGGCAG AAGGTACCCT GGTTATTGTG GTATTGATGC CACCTGAACA ACTGCTCCAG
    GATGCTCGCA GCTTCTTGCG GGCACTGGGT ACCCTGCTCC ACACCAACCT GCGCATTAAG
    CGGGACTCCC AGGGGGAACT CATGGTGTAC CCCTATTATG GTGAGAAGTC AGCTGCTATG
    AAGAAACAGA GGATGACACG CAGATCCCTT CCTGGTGAAC AAGAACAGGA GGTGGCTGGC
    TCTAAAGTCT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCACTGCTTC
    AAGAACACGG ATGCAGCAGC AGCTCTCCTG GCCTCTCACG CCATACAGGG GACCCTGTCA
    TACCCTCTTG TGTCTGTCGT CAGTGAATCC CTGACTCCAG AACGCACTCA GCTCCTCTAT
    CTCCTTGCTG TTGCTGTTGT CATCATTCTG TTTATTATTC TGCTGGGGGT AATCATGGCA
    AAACGAAAGC GTAAGCATGG CTCTCTCTGG CTGCCTGAAG GTTTCACTCT TCGCCGAGAT
    GCAAGCAATC ACAAGCGTCG TGAGCCAGTG GGACAGGATG CTGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAAGC TAACCTAATT GGTACTGGAA CAAGTGAACA CTGGGTCGAT
    GATGAAGGGC CCCAGCCAAA GAAAGTAAAG GCTGAAGATG AGGCCTTACT CTCAGAAGAA
    GATGACCCCA TTGATCGACG GCCATGGACA CAGCAGCACC TTGAAGCTGC AGACATCCGT
    AGGACACCAT CGCTGGCTCT CACCCCTCCT CAGGCAGAGC AGGAGGTGGA TGTGTTAGAT
    GTGAATGTCC GTGGCCCAGA TGGCTGCACC CCATTGATGT TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATT TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCTAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTCCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCACGGGCT GATGCTGCCA AGCGTCTCCT GGATGCAGGT
    GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGTCCAC TCCATGCTGC AGTGGCAGCT
    GATGCCCAAG GTGTCTTCCA GATTCTGATT CGCAACCGAG TAACTGATCT AGATGCCAGG
    ATGAATGATG GTACTACACC CCTGATCCTG GCTGCCCGCC TGGCTGTGGA GGGAATGGTG
    GCAGAACTGA TCAACTGCCA AGCGGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAATGTG GAGGCAACTC TTTTGTTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAGGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG
    GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGAGA CATCACAGAC
    CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGATCGCA TGCACCATGA CATTGTGCGC
    CTTCTGGATG AATACAATGT GACCCCAAGC CCTCCAGGCA CCGTGTTGAC TTCTGCTCTC
    TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TGAAGCACAC CCCAATGGGC
    AAGAAGTCTA GACGGCCCAG TGCCAAGAGT ACCATGCCTA CTAGCCTCCC TAACCTTGCC
    AAGGAGGCAA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA GAAGGTCCAA
    CTGTCTGAGA GTTCAGTAAC TTTATCCCCT GTTGATTCCC TAGAATCTCC TCACACGTAT
    GTTTCCGACA CCACATCCTC TCCAATGATT ACATCCCCTG GGATCTTACA GGCCTCACCC
    AACCCTATGT TGGCCACTGC CGCCCCTCCT GCCCCAGTCC ATGCCCAGCA TGCACTATCT
    TTTTCTAACC TTCATGAAAT GCAGCCTTTG GCACATGGGG CCAGCACTGT GCTTCCCTCA
    GTGAGCCAGT TGCTATCCCA CCACCACATT GTGTCTCCAG GCAGTGGCAG TGCTGGAAGC
    TTGAGTAGGC TCCATCCAGT CCCAGTCCCA GCAGATTGGA TGAACCGCAT GGAGGTGAAT
    GAGACCCAGT ACAATGAGAT GTTTGGTATG GTCCTGGCTC CAGCTGAGGG CACCCATCCT
    GGCATAGCTC CCCAGAGCAG GCCACCTGAA GGGAAGCACA TAACCACCCC TCGGGAGCCC
    TTGCCCCCCA TTGTGACTTT CCAGCTCATC CCTAAAGGCA GTATTGCCCA ACCAGCGGGG
    GCTCCCCAGC CTCAGTCCAC CTGCCCTCCA GCTGTTGCGG GCCCCCTGCC CACCATGTAC
    CAGATTCCAG AAATGGCCCG TTTGCCCAGT GTGGCTTTCC CCACTGCCAT GATGCCCCAG
    CAGGACGGGC AGGTAGCTCA GACCATTCTC CCAGCCTATC ATCCTTTCCC AGCCTCTGTG
    GGCAAGTACC CCACACCCCC TTCACAGCAC AGTTATGCTT CCTCAAATGC TGCTGAGCGA
    ACACCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG
    TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC
    ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT
    GAGCCACCAC ACAACAACAT GCAGGTTTAT GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19515
    Clone ID Related Accession (Same CDS sequence) NM_024408.3 , NM_024408.4
    Accession Version NM_024408.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-07
    Organism Homo sapiens(human)
    Product neurogenic locus notch homolog protein 2 isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB290699.1, BC071562.1, AF315356.1 and AL133036.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_024408.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000256646.7/ ENSP00000256646.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCCCGCCC TGCGCCCCGC TCTGCTGTGG GCGCTGCTGG CGCTCTGGCT GTGCTGCGCG 
    GCCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG
    GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC
    TGCCAGTACT CAACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA
    TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG
    GAGTGCCAAT GGACGGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG
    ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC
    CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC
    CTGTATGTGC CCTGTGCACC CTCACCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT
    GACTTCACTT TTGAGTGCAA CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT
    ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC
    ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA
    GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT
    TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTGCC
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC
    CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC
    CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT
    CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG
    TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC
    CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG
    GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT
    CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGGGTT
    AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AATCTGTATG
    GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT
    GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG
    GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA
    TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA
    TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC
    TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC
    CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG
    TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG
    CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA
    CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTAT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT
    TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT
    GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTGAATGA GGGAACGTGT
    GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT
    CAGACCCTGG TGAATCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG
    AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG
    CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC
    CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC
    TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC
    GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGCG AGTGTGTCCC AGGCTATCAG
    GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC
    ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGCCTA
    CTCTGTGAAG AGAACATTGA TGACTGTGCC CGGGGTCCCC ATTGCCTTAA TGGTGGTCAG
    TGCATGGATA GGATTGGAGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC TGGGGAGCGT
    TGTGAGGGAG ACATCAACGA GTGCCTCTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGTATACAGC TCACCAATGA CTACCTGTGT GTTTGCCGTA GTGCCTTTAC TGGCCGGCAC
    TGTGAAACCT TCGTCGATGT GTGTCCCCAG ATGCCCTGCC TGAATGGAGG GACTTGTGCT
    GTGGCCAGTA ACATGCCTGA TGGTTTCATT TGCCGTTGTC CCCCGGGATT TTCCGGGGCA
    AGGTGCCAGA GCAGCTGTGG ACAAGTGAAA TGTAGGAAGG GGGAGCAGTG TGTGCACACC
    GCCTCTGGAC CCCGCTGCTT CTGCCCCAGT CCCCGGGACT GCGAGTCAGG CTGTGCCAGT
    AGCCCCTGCC AGCACGGGGG CAGCTGCCAC CCTCAGCGCC AGCCTCCTTA TTACTCCTGC
    CAGTGTGCCC CACCATTCTC GGGTAGCCGC TGTGAACTCT ACACGGCACC CCCCAGCACC
    CCTCCTGCCA CCTGTCTGAG CCAGTATTGT GCCGACAAAG CTCGGGATGG CGTCTGTGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCTCT CACCATGGAG
    AACCCCTGGG CCAACTGCTC CTCCCCACTT CCCTGCTGGG ATTATATCAA CAACCAGTGT
    GATGAGCTGT GCAACACGGT CGAGTGCCTG TTTGACAACT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGGTGCAACA GTGAGGAGTG TGGTTGGGAT GGGCTGGACT GTGCTGCTGA CCAACCTGAG
    AACCTGGCAG AAGGTACCCT GGTTATTGTG GTATTGATGC CACCTGAACA ACTGCTCCAG
    GATGCTCGCA GCTTCTTGCG GGCACTGGGT ACCCTGCTCC ACACCAACCT GCGCATTAAG
    CGGGACTCCC AGGGGGAACT CATGGTGTAC CCCTATTATG GTGAGAAGTC AGCTGCTATG
    AAGAAACAGA GGATGACACG CAGATCCCTT CCTGGTGAAC AAGAACAGGA GGTGGCTGGC
    TCTAAAGTCT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCACTGCTTC
    AAGAACACGG ATGCAGCAGC AGCTCTCCTG GCCTCTCACG CCATACAGGG GACCCTGTCA
    TACCCTCTTG TGTCTGTCGT CAGTGAATCC CTGACTCCAG AACGCACTCA GCTCCTCTAT
    CTCCTTGCTG TTGCTGTTGT CATCATTCTG TTTATTATTC TGCTGGGGGT AATCATGGCA
    AAACGAAAGC GTAAGCATGG CTCTCTCTGG CTGCCTGAAG GTTTCACTCT TCGCCGAGAT
    GCAAGCAATC ACAAGCGTCG TGAGCCAGTG GGACAGGATG CTGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAAGC TAACCTAATT GGTACTGGAA CAAGTGAACA CTGGGTCGAT
    GATGAAGGGC CCCAGCCAAA GAAAGTAAAG GCTGAAGATG AGGCCTTACT CTCAGAAGAA
    GATGACCCCA TTGATCGACG GCCATGGACA CAGCAGCACC TTGAAGCTGC AGACATCCGT
    AGGACACCAT CGCTGGCTCT CACCCCTCCT CAGGCAGAGC AGGAGGTGGA TGTGTTAGAT
    GTGAATGTCC GTGGCCCAGA TGGCTGCACC CCATTGATGT TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATT TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCTAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTCCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCACGGGCT GATGCTGCCA AGCGTCTCCT GGATGCAGGT
    GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGTCCAC TCCATGCTGC AGTGGCAGCT
    GATGCCCAAG GTGTCTTCCA GATTCTGATT CGCAACCGAG TAACTGATCT AGATGCCAGG
    ATGAATGATG GTACTACACC CCTGATCCTG GCTGCCCGCC TGGCTGTGGA GGGAATGGTG
    GCAGAACTGA TCAACTGCCA AGCGGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAATGTG GAGGCAACTC TTTTGTTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAGGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG
    GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGAGA CATCACAGAC
    CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGATCGCA TGCACCATGA CATTGTGCGC
    CTTCTGGATG AATACAATGT GACCCCAAGC CCTCCAGGCA CCGTGTTGAC TTCTGCTCTC
    TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TGAAGCACAC CCCAATGGGC
    AAGAAGTCTA GACGGCCCAG TGCCAAGAGT ACCATGCCTA CTAGCCTCCC TAACCTTGCC
    AAGGAGGCAA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA GAAGGTCCAA
    CTGTCTGAGA GTTCAGTAAC TTTATCCCCT GTTGATTCCC TAGAATCTCC TCACACGTAT
    GTTTCCGACA CCACATCCTC TCCAATGATT ACATCCCCTG GGATCTTACA GGCCTCACCC
    AACCCTATGT TGGCCACTGC CGCCCCTCCT GCCCCAGTCC ATGCCCAGCA TGCACTATCT
    TTTTCTAACC TTCATGAAAT GCAGCCTTTG GCACATGGGG CCAGCACTGT GCTTCCCTCA
    GTGAGCCAGT TGCTATCCCA CCACCACATT GTGTCTCCAG GCAGTGGCAG TGCTGGAAGC
    TTGAGTAGGC TCCATCCAGT CCCAGTCCCA GCAGATTGGA TGAACCGCAT GGAGGTGAAT
    GAGACCCAGT ACAATGAGAT GTTTGGTATG GTCCTGGCTC CAGCTGAGGG CACCCATCCT
    GGCATAGCTC CCCAGAGCAG GCCACCTGAA GGGAAGCACA TAACCACCCC TCGGGAGCCC
    TTGCCCCCCA TTGTGACTTT CCAGCTCATC CCTAAAGGCA GTATTGCCCA ACCAGCGGGG
    GCTCCCCAGC CTCAGTCCAC CTGCCCTCCA GCTGTTGCGG GCCCCCTGCC CACCATGTAC
    CAGATTCCAG AAATGGCCCG TTTGCCCAGT GTGGCTTTCC CCACTGCCAT GATGCCCCAG
    CAGGACGGGC AGGTAGCTCA GACCATTCTC CCAGCCTATC ATCCTTTCCC AGCCTCTGTG
    GGCAAGTACC CCACACCCCC TTCACAGCAC AGTTATGCTT CCTCAAATGC TGCTGAGCGA
    ACACCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG
    TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC
    ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT
    GAGCCACCAC ACAACAACAT GCAGGTTTAT GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_077719.2
    CDS257..7672
    Translation

    Target ORF information:

    RefSeq Version NM_024408.4
    Organism Homo sapiens(human)
    Definition Homo sapiens notch receptor 2 (NOTCH2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_024408.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCCCGCCC TGCGCCCCGC TCTGCTGTGG GCGCTGCTGG CGCTCTGGCT GTGCTGCGCG 
    GCCCCCGCGC ATGCATTGCA GTGTCGAGAT GGCTATGAAC CCTGTGTAAA TGAAGGAATG
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAAGGCTT CTTGGGGGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAAG AACCGCTGCC AGAATGGTGG GACTTGTGTG
    GCCCAGGCCA TGCTGGGGAA AGCCACGTGC CGATGTGCCT CAGGGTTTAC AGGAGAGGAC
    TGCCAGTACT CAACATCTCA TCCATGCTTT GTGTCTCGAC CCTGCCTGAA TGGCGGCACA
    TGCCATATGC TCAGCCGGGA TACCTATGAG TGCACCTGTC AAGTCGGGTT TACAGGTAAG
    GAGTGCCAAT GGACGGATGC CTGCCTGTCT CATCCCTGTG CAAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACAGGGCA GAAATGTGAG
    ACTGATGTCA ATGAGTGTGA CATTCCAGGA CACTGCCAGC ATGGTGGCAC CTGCCTCAAC
    CTGCCTGGTT CCTACCAGTG CCAGTGCCCT CAGGGCTTCA CAGGCCAGTA CTGTGACAGC
    CTGTATGTGC CCTGTGCACC CTCACCTTGT GTCAATGGAG GCACCTGTCG GCAGACTGGT
    GACTTCACTT TTGAGTGCAA CTGCCTTCCA GGTTTTGAAG GGAGCACCTG TGAGAGGAAT
    ATTGATGACT GCCCTAACCA CAGGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTCAAC
    ACTTACAACT GCCGCTGTCC CCCACAATGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAATGCCTGC TGCAGCCCAA TGCCTGTCAA AATGGGGGCA CCTGTGCCAA CCGCAATGGA
    GGCTATGGCT GTGTATGTGT CAACGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GATTGTGCCT TCGCCTCCTG TACTCCAGGC TCCACCTGCA TCGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAGGG GAAGGCAGGT CTCCTGTGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GACACCAACC CCCTAAATGG GCAATATATT
    TGCACCTGCC CACAAGGCTA CAAAGGGGCT GACTGCACAG AAGATGTGGA TGAATGTGCC
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAATGTG TGAACACGGA TGGCGCCTTC
    CACTGTGAGT GTCTGAAGGG TTATGCAGGA CCTCGTTGTG AGATGGACAT CAATGAGTGC
    CATTCAGACC CCTGCCAGAA TGATGCTACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    CTGTGCATGC CAGGTTTCAA AGGTGTGCAT TGTGAATTAG AAATAAATGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG GCAGTGTGTG GATAAAGTCA ATCGTTTCCA GTGCCTGTGT
    CCTCCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCG
    TGTCTGAATG GGGCAAAGTG TATCGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGTTTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCCGA TCCTTGCCAC
    CATGGTCAGT GTCAGGATGG TATTGATTCC TACACCTGCA TCTGCAATCC CGGGTACATG
    GGCGCCATCT GCAGTGACCA GATTGATGAA TGTTACAGCA GCCCTTGCCT GAACGATGGT
    CGCTGCATTG ACCTGGTCAA TGGCTACCAG TGCAACTGCC AGCCAGGCAC GTCAGGGGTT
    AATTGTGAAA TTAATTTTGA TGACTGTGCA AGTAACCCTT GTATCCATGG AATCTGTATG
    GATGGCATTA ATCGCTACAG TTGTGTCTGC TCACCAGGAT TCACAGGGCA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG TATCAACGGT
    GTGAATGGTT TCCGCTGTAT ATGCCCCGAG GGACCCCATC ACCCCAGCTG CTACTCACAG
    GTGAACGAAT GCCTGAGCAA TCCCTGCATC CATGGAAACT GTACTGGAGG TCTCAGTGGA
    TATAAGTGTC TCTGTGATGC AGGCTGGGTT GGCATCAACT GTGAAGTGGA CAAAAATGAA
    TGCCTTTCGA ATCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGCCAGG TGAATATTGA TGAATGTGCC
    TCAAATCCAT GCCTGAACCA AGGAACCTGC TTTGATGACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACAGTAT TGGCTCCCTG TTCCCCAAAC
    CCTTGTGAGA ATGCTGCTGT TTGCAAAGAG TCACCAAATT TTGAGAGTTA TACTTGCTTG
    TGTGCTCCTG GCTGGCAAGG TCAGCGGTGT ACCATTGACA TTGACGAGTG TATCTCCAAG
    CCCTGCATGA ACCATGGTCT CTGCCATAAC ACCCAGGGCA GCTACATGTG TGAATGTCCA
    CCAGGCTTCA GTGGTATGGA CTGTGAGGAG GACATTGATG ACTGCCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTAT GGATGGAGTG AATACTTTCT CCTGCCTCTG CCTTCCGGGT
    TTCACTGGGG ATAAGTGCCA GACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACCT GCTCTGACTA CGTCAACAGT TACACTTGCA AGTGCCAGGC AGGATTTGAT
    GGAGTCCATT GTGAGAACAA CATCAATGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTTG ATGGGATTAA CTCCTTCTCT TGCTTGTGCC CTGTGGGTTT CACTGGATCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAGC TCTCATCCAT GCCTGAATGA GGGAACGTGT
    GTTGATGGCC TGGGTACCTA CCGCTGCAGC TGCCCCCTGG GCTACACTGG GAAAAACTGT
    CAGACCCTGG TGAATCTCTG CAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCGTTCAG
    AAAAAAGCAG AGTCCCAGTG CCTATGTCCA TCTGGATGGG CTGGTGCCTA TTGTGACGTG
    CCCAATGTCT CTTGTGACAT AGCAGCCTCC AGGAGAGGTG TGCTTGTTGA ACACTTGTGC
    CAGCACTCAG GTGTCTGCAT CAATGCTGGC AACACGCATT ACTGTCAGTG CCCCCTGGGC
    TATACTGGGA GCTACTGTGA GGAGCAACTC GATGAGTGTG CGTCCAACCC CTGCCAGCAC
    GGGGCAACAT GCAGTGACTT CATTGGTGGA TACAGATGCG AGTGTGTCCC AGGCTATCAG
    GGTGTCAACT GTGAGTATGA AGTGGATGAG TGCCAGAATC AGCCCTGCCA GAATGGAGGC
    ACCTGTATTG ACCTTGTGAA CCATTTCAAG TGCTCTTGCC CACCAGGCAC TCGGGGCCTA
    CTCTGTGAAG AGAACATTGA TGACTGTGCC CGGGGTCCCC ATTGCCTTAA TGGTGGTCAG
    TGCATGGATA GGATTGGAGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC TGGGGAGCGT
    TGTGAGGGAG ACATCAACGA GTGCCTCTCC AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGTATACAGC TCACCAATGA CTACCTGTGT GTTTGCCGTA GTGCCTTTAC TGGCCGGCAC
    TGTGAAACCT TCGTCGATGT GTGTCCCCAG ATGCCCTGCC TGAATGGAGG GACTTGTGCT
    GTGGCCAGTA ACATGCCTGA TGGTTTCATT TGCCGTTGTC CCCCGGGATT TTCCGGGGCA
    AGGTGCCAGA GCAGCTGTGG ACAAGTGAAA TGTAGGAAGG GGGAGCAGTG TGTGCACACC
    GCCTCTGGAC CCCGCTGCTT CTGCCCCAGT CCCCGGGACT GCGAGTCAGG CTGTGCCAGT
    AGCCCCTGCC AGCACGGGGG CAGCTGCCAC CCTCAGCGCC AGCCTCCTTA TTACTCCTGC
    CAGTGTGCCC CACCATTCTC GGGTAGCCGC TGTGAACTCT ACACGGCACC CCCCAGCACC
    CCTCCTGCCA CCTGTCTGAG CCAGTATTGT GCCGACAAAG CTCGGGATGG CGTCTGTGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCTCT CACCATGGAG
    AACCCCTGGG CCAACTGCTC CTCCCCACTT CCCTGCTGGG ATTATATCAA CAACCAGTGT
    GATGAGCTGT GCAACACGGT CGAGTGCCTG TTTGACAACT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGGTGCAACA GTGAGGAGTG TGGTTGGGAT GGGCTGGACT GTGCTGCTGA CCAACCTGAG
    AACCTGGCAG AAGGTACCCT GGTTATTGTG GTATTGATGC CACCTGAACA ACTGCTCCAG
    GATGCTCGCA GCTTCTTGCG GGCACTGGGT ACCCTGCTCC ACACCAACCT GCGCATTAAG
    CGGGACTCCC AGGGGGAACT CATGGTGTAC CCCTATTATG GTGAGAAGTC AGCTGCTATG
    AAGAAACAGA GGATGACACG CAGATCCCTT CCTGGTGAAC AAGAACAGGA GGTGGCTGGC
    TCTAAAGTCT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGACTCAGA CCACTGCTTC
    AAGAACACGG ATGCAGCAGC AGCTCTCCTG GCCTCTCACG CCATACAGGG GACCCTGTCA
    TACCCTCTTG TGTCTGTCGT CAGTGAATCC CTGACTCCAG AACGCACTCA GCTCCTCTAT
    CTCCTTGCTG TTGCTGTTGT CATCATTCTG TTTATTATTC TGCTGGGGGT AATCATGGCA
    AAACGAAAGC GTAAGCATGG CTCTCTCTGG CTGCCTGAAG GTTTCACTCT TCGCCGAGAT
    GCAAGCAATC ACAAGCGTCG TGAGCCAGTG GGACAGGATG CTGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAAGC TAACCTAATT GGTACTGGAA CAAGTGAACA CTGGGTCGAT
    GATGAAGGGC CCCAGCCAAA GAAAGTAAAG GCTGAAGATG AGGCCTTACT CTCAGAAGAA
    GATGACCCCA TTGATCGACG GCCATGGACA CAGCAGCACC TTGAAGCTGC AGACATCCGT
    AGGACACCAT CGCTGGCTCT CACCCCTCCT CAGGCAGAGC AGGAGGTGGA TGTGTTAGAT
    GTGAATGTCC GTGGCCCAGA TGGCTGCACC CCATTGATGT TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATT TGAGTGATGA AGATGAAGAT GCAGAGGACT CTTCTGCTAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTCCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCACGGGCT GATGCTGCCA AGCGTCTCCT GGATGCAGGT
    GCAGATGCCA ATGCCCAGGA CAACATGGGC CGCTGTCCAC TCCATGCTGC AGTGGCAGCT
    GATGCCCAAG GTGTCTTCCA GATTCTGATT CGCAACCGAG TAACTGATCT AGATGCCAGG
    ATGAATGATG GTACTACACC CCTGATCCTG GCTGCCCGCC TGGCTGTGGA GGGAATGGTG
    GCAGAACTGA TCAACTGCCA AGCGGATGTG AATGCAGTGG ATGACCATGG AAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAATGTG GAGGCAACTC TTTTGTTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAGGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG
    GGGAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGAGA CATCACAGAC
    CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGATCGCA TGCACCATGA CATTGTGCGC
    CTTCTGGATG AATACAATGT GACCCCAAGC CCTCCAGGCA CCGTGTTGAC TTCTGCTCTC
    TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TGAAGCACAC CCCAATGGGC
    AAGAAGTCTA GACGGCCCAG TGCCAAGAGT ACCATGCCTA CTAGCCTCCC TAACCTTGCC
    AAGGAGGCAA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA GAAGGTCCAA
    CTGTCTGAGA GTTCAGTAAC TTTATCCCCT GTTGATTCCC TAGAATCTCC TCACACGTAT
    GTTTCCGACA CCACATCCTC TCCAATGATT ACATCCCCTG GGATCTTACA GGCCTCACCC
    AACCCTATGT TGGCCACTGC CGCCCCTCCT GCCCCAGTCC ATGCCCAGCA TGCACTATCT
    TTTTCTAACC TTCATGAAAT GCAGCCTTTG GCACATGGGG CCAGCACTGT GCTTCCCTCA
    GTGAGCCAGT TGCTATCCCA CCACCACATT GTGTCTCCAG GCAGTGGCAG TGCTGGAAGC
    TTGAGTAGGC TCCATCCAGT CCCAGTCCCA GCAGATTGGA TGAACCGCAT GGAGGTGAAT
    GAGACCCAGT ACAATGAGAT GTTTGGTATG GTCCTGGCTC CAGCTGAGGG CACCCATCCT
    GGCATAGCTC CCCAGAGCAG GCCACCTGAA GGGAAGCACA TAACCACCCC TCGGGAGCCC
    TTGCCCCCCA TTGTGACTTT CCAGCTCATC CCTAAAGGCA GTATTGCCCA ACCAGCGGGG
    GCTCCCCAGC CTCAGTCCAC CTGCCCTCCA GCTGTTGCGG GCCCCCTGCC CACCATGTAC
    CAGATTCCAG AAATGGCCCG TTTGCCCAGT GTGGCTTTCC CCACTGCCAT GATGCCCCAG
    CAGGACGGGC AGGTAGCTCA GACCATTCTC CCAGCCTATC ATCCTTTCCC AGCCTCTGTG
    GGCAAGTACC CCACACCCCC TTCACAGCAC AGTTATGCTT CCTCAAATGC TGCTGAGCGA
    ACACCCAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG
    TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC
    ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGGAC ACACATGTCT
    GAGCCACCAC ACAACAACAT GCAGGTTTAT GCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu33467
    Clone ID Related Accession (Same CDS sequence) XM_005270901.3 , XM_011541520.2 , XM_017001373.1
    Accession Version XM_005270901.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurogenic locus notch homolog protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005270901.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
    GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT
    GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG
    GACTGCCAGT ACTCAACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC
    ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT
    AAGGAGTGCC AATGGACGGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT
    ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAATGT
    GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC
    AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC
    AGCCTGTATG TGCCCTGTGC ACCCTCACCT TGTGTCAATG GAGGCACCTG TCGGCAGACT
    GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG
    AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC
    AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG
    GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT
    GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT
    GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC
    TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC
    ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT
    ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT
    GCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC
    TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG
    TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA
    TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG
    AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG
    TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT
    CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC
    ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC
    CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC
    ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT
    GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCAGG CACGTCAGGG
    GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAATCTGT
    ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT
    AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC
    GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA
    CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT
    GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT
    GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC
    AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT
    GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC
    CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA
    AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC
    TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC
    AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT
    CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT
    TGCCAGAATG GAGGTTCCTG TATGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG
    GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG
    AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT
    GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT
    GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA
    TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTGAA TGAGGGAACG
    TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC
    TGTCAGACCC TGGTGAATCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT
    CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC
    GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG
    TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG
    GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG
    CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GCGAGTGTGT CCCAGGCTAT
    CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA
    GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC
    CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT
    CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG
    CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG
    GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG
    CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT
    GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCCGGG
    GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AGGGGGAGCA GTGTGTGCAC
    ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC
    AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC
    TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC
    ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT
    GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG
    GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG
    TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC
    AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC
    CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT
    GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC
    CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TCCACACCAA CCTGCGCATT
    AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT
    ATGAAGAAAC AGAGGATGAC ACGCAGATCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT
    GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC
    TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG
    TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC
    TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG
    GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA
    GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTGAAAAAT
    CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTACTG GAACAAGTGA ACACTGGGTC
    GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA
    GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC TGCAGACATC
    CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA
    GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA
    GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC
    ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG
    GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA
    GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGTC CACTCCATGC TGCAGTGGCA
    GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC
    AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG
    GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT
    GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT
    GGGGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG
    GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA
    GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG
    CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT
    CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG
    GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT
    GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC
    CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG
    TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA
    CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA
    TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC
    TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA
    AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG
    AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT
    CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG
    CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG
    GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG
    TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC
    CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT
    GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG
    CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA
    GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG
    ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG
    TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005270958.1
    CDS2729..10027
    Translation

    Target ORF information:

    RefSeq Version XM_005270901.3
    Organism Homo sapiens(human)
    Definition Homo sapiens notch 2 (NOTCH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005270901.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
    GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT
    GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG
    GACTGCCAGT ACTCAACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC
    ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT
    AAGGAGTGCC AATGGACGGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT
    ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAATGT
    GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC
    AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC
    AGCCTGTATG TGCCCTGTGC ACCCTCACCT TGTGTCAATG GAGGCACCTG TCGGCAGACT
    GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG
    AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC
    AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG
    GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT
    GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT
    GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC
    TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC
    ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT
    ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT
    GCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC
    TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG
    TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA
    TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG
    AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG
    TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT
    CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC
    ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC
    CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC
    ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT
    GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCAGG CACGTCAGGG
    GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAATCTGT
    ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT
    AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC
    GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA
    CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT
    GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT
    GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC
    AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT
    GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC
    CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA
    AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC
    TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC
    AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT
    CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT
    TGCCAGAATG GAGGTTCCTG TATGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG
    GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG
    AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT
    GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT
    GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA
    TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTGAA TGAGGGAACG
    TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC
    TGTCAGACCC TGGTGAATCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT
    CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC
    GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG
    TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG
    GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG
    CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GCGAGTGTGT CCCAGGCTAT
    CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA
    GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC
    CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT
    CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG
    CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG
    GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG
    CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT
    GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCCGGG
    GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AGGGGGAGCA GTGTGTGCAC
    ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC
    AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC
    TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC
    ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT
    GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG
    GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG
    TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC
    AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC
    CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT
    GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC
    CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TCCACACCAA CCTGCGCATT
    AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT
    ATGAAGAAAC AGAGGATGAC ACGCAGATCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT
    GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC
    TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG
    TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC
    TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG
    GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA
    GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTGAAAAAT
    CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTACTG GAACAAGTGA ACACTGGGTC
    GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA
    GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC TGCAGACATC
    CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA
    GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA
    GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC
    ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG
    GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA
    GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGTC CACTCCATGC TGCAGTGGCA
    GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC
    AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG
    GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT
    GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT
    GGGGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG
    GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA
    GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG
    CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT
    CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG
    GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT
    GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC
    CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG
    TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA
    CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA
    TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC
    TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA
    AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG
    AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT
    CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG
    CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG
    GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG
    TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC
    CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT
    GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG
    CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA
    GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG
    ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG
    TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu33467
    Clone ID Related Accession (Same CDS sequence) XM_005270901.3 , XM_011541520.2 , XM_017001373.1
    Accession Version XM_011541520.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurogenic locus notch homolog protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
    GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT
    GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG
    GACTGCCAGT ACTCAACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC
    ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT
    AAGGAGTGCC AATGGACGGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT
    ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAATGT
    GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC
    AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC
    AGCCTGTATG TGCCCTGTGC ACCCTCACCT TGTGTCAATG GAGGCACCTG TCGGCAGACT
    GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG
    AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC
    AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG
    GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT
    GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT
    GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC
    TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC
    ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT
    ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT
    GCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC
    TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG
    TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA
    TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG
    AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG
    TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT
    CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC
    ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC
    CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC
    ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT
    GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCAGG CACGTCAGGG
    GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAATCTGT
    ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT
    AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC
    GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA
    CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT
    GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT
    GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC
    AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT
    GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC
    CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA
    AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC
    TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC
    AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT
    CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT
    TGCCAGAATG GAGGTTCCTG TATGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG
    GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG
    AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT
    GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT
    GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA
    TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTGAA TGAGGGAACG
    TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC
    TGTCAGACCC TGGTGAATCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT
    CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC
    GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG
    TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG
    GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG
    CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GCGAGTGTGT CCCAGGCTAT
    CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA
    GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC
    CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT
    CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG
    CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG
    GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG
    CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT
    GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCCGGG
    GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AGGGGGAGCA GTGTGTGCAC
    ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC
    AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC
    TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC
    ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT
    GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG
    GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG
    TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC
    AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC
    CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT
    GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC
    CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TCCACACCAA CCTGCGCATT
    AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT
    ATGAAGAAAC AGAGGATGAC ACGCAGATCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT
    GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC
    TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG
    TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC
    TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG
    GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA
    GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTGAAAAAT
    CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTACTG GAACAAGTGA ACACTGGGTC
    GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA
    GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC TGCAGACATC
    CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA
    GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA
    GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC
    ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG
    GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA
    GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGTC CACTCCATGC TGCAGTGGCA
    GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC
    AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG
    GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT
    GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT
    GGGGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG
    GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA
    GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG
    CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT
    CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG
    GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT
    GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC
    CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG
    TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA
    CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA
    TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC
    TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA
    AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG
    AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT
    CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG
    CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG
    GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG
    TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC
    CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT
    GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG
    CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA
    GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG
    ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG
    TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539822.1
    CDS2433..9731
    Translation

    Target ORF information:

    RefSeq Version XM_011541520.2
    Organism Homo sapiens(human)
    Definition Homo sapiens notch 2 (NOTCH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011541520.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
    GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT
    GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG
    GACTGCCAGT ACTCAACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC
    ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT
    AAGGAGTGCC AATGGACGGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT
    ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAATGT
    GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC
    AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC
    AGCCTGTATG TGCCCTGTGC ACCCTCACCT TGTGTCAATG GAGGCACCTG TCGGCAGACT
    GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG
    AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC
    AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG
    GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT
    GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT
    GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC
    TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC
    ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT
    ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT
    GCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC
    TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG
    TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA
    TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG
    AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG
    TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT
    CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC
    ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC
    CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC
    ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT
    GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCAGG CACGTCAGGG
    GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAATCTGT
    ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT
    AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC
    GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA
    CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT
    GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT
    GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC
    AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT
    GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC
    CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA
    AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC
    TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC
    AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT
    CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT
    TGCCAGAATG GAGGTTCCTG TATGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG
    GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG
    AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT
    GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT
    GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA
    TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTGAA TGAGGGAACG
    TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC
    TGTCAGACCC TGGTGAATCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT
    CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC
    GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG
    TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG
    GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG
    CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GCGAGTGTGT CCCAGGCTAT
    CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA
    GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC
    CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT
    CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG
    CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG
    GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG
    CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT
    GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCCGGG
    GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AGGGGGAGCA GTGTGTGCAC
    ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC
    AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC
    TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC
    ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT
    GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG
    GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG
    TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC
    AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC
    CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT
    GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC
    CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TCCACACCAA CCTGCGCATT
    AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT
    ATGAAGAAAC AGAGGATGAC ACGCAGATCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT
    GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC
    TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG
    TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC
    TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG
    GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA
    GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTGAAAAAT
    CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTACTG GAACAAGTGA ACACTGGGTC
    GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA
    GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC TGCAGACATC
    CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA
    GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA
    GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC
    ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG
    GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA
    GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGTC CACTCCATGC TGCAGTGGCA
    GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC
    AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG
    GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT
    GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT
    GGGGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG
    GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA
    GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG
    CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT
    CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG
    GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT
    GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC
    CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG
    TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA
    CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA
    TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC
    TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA
    AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG
    AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT
    CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG
    CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG
    GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG
    TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC
    CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT
    GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG
    CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA
    GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG
    ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG
    TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu33467
    Clone ID Related Accession (Same CDS sequence) XM_005270901.3 , XM_011541520.2 , XM_017001373.1
    Accession Version XM_017001373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurogenic locus notch homolog protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
    GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT
    GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG
    GACTGCCAGT ACTCAACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC
    ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT
    AAGGAGTGCC AATGGACGGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT
    ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAATGT
    GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC
    AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC
    AGCCTGTATG TGCCCTGTGC ACCCTCACCT TGTGTCAATG GAGGCACCTG TCGGCAGACT
    GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG
    AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC
    AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG
    GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT
    GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT
    GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC
    TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC
    ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT
    ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT
    GCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC
    TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG
    TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA
    TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG
    AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG
    TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT
    CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC
    ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC
    CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC
    ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT
    GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCAGG CACGTCAGGG
    GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAATCTGT
    ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT
    AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC
    GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA
    CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT
    GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT
    GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC
    AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT
    GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC
    CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA
    AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC
    TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC
    AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT
    CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT
    TGCCAGAATG GAGGTTCCTG TATGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG
    GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG
    AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT
    GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT
    GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA
    TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTGAA TGAGGGAACG
    TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC
    TGTCAGACCC TGGTGAATCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT
    CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC
    GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG
    TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG
    GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG
    CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GCGAGTGTGT CCCAGGCTAT
    CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA
    GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC
    CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT
    CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG
    CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG
    GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG
    CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT
    GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCCGGG
    GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AGGGGGAGCA GTGTGTGCAC
    ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC
    AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC
    TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC
    ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT
    GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG
    GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG
    TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC
    AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC
    CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT
    GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC
    CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TCCACACCAA CCTGCGCATT
    AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT
    ATGAAGAAAC AGAGGATGAC ACGCAGATCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT
    GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC
    TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG
    TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC
    TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG
    GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA
    GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTGAAAAAT
    CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTACTG GAACAAGTGA ACACTGGGTC
    GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA
    GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC TGCAGACATC
    CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA
    GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA
    GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC
    ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG
    GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA
    GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGTC CACTCCATGC TGCAGTGGCA
    GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC
    AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG
    GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT
    GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT
    GGGGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG
    GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA
    GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG
    CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT
    CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG
    GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT
    GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC
    CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG
    TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA
    CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA
    TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC
    TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA
    AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG
    AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT
    CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG
    CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG
    GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG
    TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC
    CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT
    GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG
    CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA
    GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG
    ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG
    TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016856862.1
    CDS64..7362
    Translation

    Target ORF information:

    RefSeq Version XM_017001373.1
    Organism Homo sapiens(human)
    Definition Homo sapiens notch 2 (NOTCH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017001373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGTGTGTTA CCTACCACAA TGGCACAGGA TACTGCAAAT GTCCAGAAGG CTTCTTGGGG 
    GAATATTGTC AACATCGAGA CCCCTGTGAG AAGAACCGCT GCCAGAATGG TGGGACTTGT
    GTGGCCCAGG CCATGCTGGG GAAAGCCACG TGCCGATGTG CCTCAGGGTT TACAGGAGAG
    GACTGCCAGT ACTCAACATC TCATCCATGC TTTGTGTCTC GACCCTGCCT GAATGGCGGC
    ACATGCCATA TGCTCAGCCG GGATACCTAT GAGTGCACCT GTCAAGTCGG GTTTACAGGT
    AAGGAGTGCC AATGGACGGA TGCCTGCCTG TCTCATCCCT GTGCAAATGG AAGTACCTGT
    ACCACTGTGG CCAACCAGTT CTCCTGCAAA TGCCTCACAG GCTTCACAGG GCAGAAATGT
    GAGACTGATG TCAATGAGTG TGACATTCCA GGACACTGCC AGCATGGTGG CACCTGCCTC
    AACCTGCCTG GTTCCTACCA GTGCCAGTGC CCTCAGGGCT TCACAGGCCA GTACTGTGAC
    AGCCTGTATG TGCCCTGTGC ACCCTCACCT TGTGTCAATG GAGGCACCTG TCGGCAGACT
    GGTGACTTCA CTTTTGAGTG CAACTGCCTT CCAGGTTTTG AAGGGAGCAC CTGTGAGAGG
    AATATTGATG ACTGCCCTAA CCACAGGTGT CAGAATGGAG GGGTTTGTGT GGATGGGGTC
    AACACTTACA ACTGCCGCTG TCCCCCACAA TGGACAGGAC AGTTCTGCAC AGAGGATGTG
    GATGAATGCC TGCTGCAGCC CAATGCCTGT CAAAATGGGG GCACCTGTGC CAACCGCAAT
    GGAGGCTATG GCTGTGTATG TGTCAACGGC TGGAGTGGAG ATGACTGCAG TGAGAACATT
    GATGATTGTG CCTTCGCCTC CTGTACTCCA GGCTCCACCT GCATCGACCG TGTGGCCTCC
    TTCTCTTGCA TGTGCCCAGA GGGGAAGGCA GGTCTCCTGT GTCATCTGGA TGATGCATGC
    ATCAGCAATC CTTGCCACAA GGGGGCACTG TGTGACACCA ACCCCCTAAA TGGGCAATAT
    ATTTGCACCT GCCCACAAGG CTACAAAGGG GCTGACTGCA CAGAAGATGT GGATGAATGT
    GCCATGGCCA ATAGCAATCC TTGTGAGCAT GCAGGAAAAT GTGTGAACAC GGATGGCGCC
    TTCCACTGTG AGTGTCTGAA GGGTTATGCA GGACCTCGTT GTGAGATGGA CATCAATGAG
    TGCCATTCAG ACCCCTGCCA GAATGATGCT ACCTGTCTGG ATAAGATTGG AGGCTTCACA
    TGTCTGTGCA TGCCAGGTTT CAAAGGTGTG CATTGTGAAT TAGAAATAAA TGAATGTCAG
    AGCAACCCTT GTGTGAACAA TGGGCAGTGT GTGGATAAAG TCAATCGTTT CCAGTGCCTG
    TGTCCTCCTG GTTTCACTGG GCCAGTTTGC CAGATTGATA TTGATGACTG TTCCAGTACT
    CCGTGTCTGA ATGGGGCAAA GTGTATCGAT CACCCGAATG GCTATGAATG CCAGTGTGCC
    ACAGGTTTCA CTGGTGTGTT GTGTGAGGAG AACATTGACA ACTGTGACCC CGATCCTTGC
    CACCATGGTC AGTGTCAGGA TGGTATTGAT TCCTACACCT GCATCTGCAA TCCCGGGTAC
    ATGGGCGCCA TCTGCAGTGA CCAGATTGAT GAATGTTACA GCAGCCCTTG CCTGAACGAT
    GGTCGCTGCA TTGACCTGGT CAATGGCTAC CAGTGCAACT GCCAGCCAGG CACGTCAGGG
    GTTAATTGTG AAATTAATTT TGATGACTGT GCAAGTAACC CTTGTATCCA TGGAATCTGT
    ATGGATGGCA TTAATCGCTA CAGTTGTGTC TGCTCACCAG GATTCACAGG GCAGAGATGT
    AACATTGACA TTGATGAGTG TGCCTCCAAT CCCTGTCGCA AGGGTGCAAC ATGTATCAAC
    GGTGTGAATG GTTTCCGCTG TATATGCCCC GAGGGACCCC ATCACCCCAG CTGCTACTCA
    CAGGTGAACG AATGCCTGAG CAATCCCTGC ATCCATGGAA ACTGTACTGG AGGTCTCAGT
    GGATATAAGT GTCTCTGTGA TGCAGGCTGG GTTGGCATCA ACTGTGAAGT GGACAAAAAT
    GAATGCCTTT CGAATCCATG CCAGAATGGA GGAACTTGTG ACAATCTGGT GAATGGATAC
    AGGTGTACTT GCAAGAAGGG CTTTAAAGGC TATAACTGCC AGGTGAATAT TGATGAATGT
    GCCTCAAATC CATGCCTGAA CCAAGGAACC TGCTTTGATG ACATAAGTGG CTACACTTGC
    CACTGTGTGC TGCCATACAC AGGCAAGAAT TGTCAGACAG TATTGGCTCC CTGTTCCCCA
    AACCCTTGTG AGAATGCTGC TGTTTGCAAA GAGTCACCAA ATTTTGAGAG TTATACTTGC
    TTGTGTGCTC CTGGCTGGCA AGGTCAGCGG TGTACCATTG ACATTGACGA GTGTATCTCC
    AAGCCCTGCA TGAACCATGG TCTCTGCCAT AACACCCAGG GCAGCTACAT GTGTGAATGT
    CCACCAGGCT TCAGTGGTAT GGACTGTGAG GAGGACATTG ATGACTGCCT TGCCAATCCT
    TGCCAGAATG GAGGTTCCTG TATGGATGGA GTGAATACTT TCTCCTGCCT CTGCCTTCCG
    GGTTTCACTG GGGATAAGTG CCAGACAGAC ATGAATGAGT GTCTGAGTGA ACCCTGTAAG
    AATGGAGGGA CCTGCTCTGA CTACGTCAAC AGTTACACTT GCAAGTGCCA GGCAGGATTT
    GATGGAGTCC ATTGTGAGAA CAACATCAAT GAGTGCACTG AGAGCTCCTG TTTCAATGGT
    GGCACATGTG TTGATGGGAT TAACTCCTTC TCTTGCTTGT GCCCTGTGGG TTTCACTGGA
    TCCTTCTGCC TCCATGAGAT CAATGAATGC AGCTCTCATC CATGCCTGAA TGAGGGAACG
    TGTGTTGATG GCCTGGGTAC CTACCGCTGC AGCTGCCCCC TGGGCTACAC TGGGAAAAAC
    TGTCAGACCC TGGTGAATCT CTGCAGTCGG TCTCCATGTA AAAACAAAGG TACTTGCGTT
    CAGAAAAAAG CAGAGTCCCA GTGCCTATGT CCATCTGGAT GGGCTGGTGC CTATTGTGAC
    GTGCCCAATG TCTCTTGTGA CATAGCAGCC TCCAGGAGAG GTGTGCTTGT TGAACACTTG
    TGCCAGCACT CAGGTGTCTG CATCAATGCT GGCAACACGC ATTACTGTCA GTGCCCCCTG
    GGCTATACTG GGAGCTACTG TGAGGAGCAA CTCGATGAGT GTGCGTCCAA CCCCTGCCAG
    CACGGGGCAA CATGCAGTGA CTTCATTGGT GGATACAGAT GCGAGTGTGT CCCAGGCTAT
    CAGGGTGTCA ACTGTGAGTA TGAAGTGGAT GAGTGCCAGA ATCAGCCCTG CCAGAATGGA
    GGCACCTGTA TTGACCTTGT GAACCATTTC AAGTGCTCTT GCCCACCAGG CACTCGGGGC
    CTACTCTGTG AAGAGAACAT TGATGACTGT GCCCGGGGTC CCCATTGCCT TAATGGTGGT
    CAGTGCATGG ATAGGATTGG AGGCTACAGT TGTCGCTGCT TGCCTGGCTT TGCTGGGGAG
    CGTTGTGAGG GAGACATCAA CGAGTGCCTC TCCAACCCCT GCAGCTCTGA GGGCAGCCTG
    GACTGTATAC AGCTCACCAA TGACTACCTG TGTGTTTGCC GTAGTGCCTT TACTGGCCGG
    CACTGTGAAA CCTTCGTCGA TGTGTGTCCC CAGATGCCCT GCCTGAATGG AGGGACTTGT
    GCTGTGGCCA GTAACATGCC TGATGGTTTC ATTTGCCGTT GTCCCCCGGG ATTTTCCGGG
    GCAAGGTGCC AGAGCAGCTG TGGACAAGTG AAATGTAGGA AGGGGGAGCA GTGTGTGCAC
    ACCGCCTCTG GACCCCGCTG CTTCTGCCCC AGTCCCCGGG ACTGCGAGTC AGGCTGTGCC
    AGTAGCCCCT GCCAGCACGG GGGCAGCTGC CACCCTCAGC GCCAGCCTCC TTATTACTCC
    TGCCAGTGTG CCCCACCATT CTCGGGTAGC CGCTGTGAAC TCTACACGGC ACCCCCCAGC
    ACCCCTCCTG CCACCTGTCT GAGCCAGTAT TGTGCCGACA AAGCTCGGGA TGGCGTCTGT
    GATGAGGCCT GCAACAGCCA TGCCTGCCAG TGGGATGGGG GTGACTGTTC TCTCACCATG
    GAGAACCCCT GGGCCAACTG CTCCTCCCCA CTTCCCTGCT GGGATTATAT CAACAACCAG
    TGTGATGAGC TGTGCAACAC GGTCGAGTGC CTGTTTGACA ACTTTGAATG CCAGGGGAAC
    AGCAAGACAT GCAAGTATGA CAAATACTGT GCAGACCACT TCAAAGACAA CCACTGTGAC
    CAGGGGTGCA ACAGTGAGGA GTGTGGTTGG GATGGGCTGG ACTGTGCTGC TGACCAACCT
    GAGAACCTGG CAGAAGGTAC CCTGGTTATT GTGGTATTGA TGCCACCTGA ACAACTGCTC
    CAGGATGCTC GCAGCTTCTT GCGGGCACTG GGTACCCTGC TCCACACCAA CCTGCGCATT
    AAGCGGGACT CCCAGGGGGA ACTCATGGTG TACCCCTATT ATGGTGAGAA GTCAGCTGCT
    ATGAAGAAAC AGAGGATGAC ACGCAGATCC CTTCCTGGTG AACAAGAACA GGAGGTGGCT
    GGCTCTAAAG TCTTTCTGGA AATTGACAAC CGCCAGTGTG TTCAAGACTC AGACCACTGC
    TTCAAGAACA CGGATGCAGC AGCAGCTCTC CTGGCCTCTC ACGCCATACA GGGGACCCTG
    TCATACCCTC TTGTGTCTGT CGTCAGTGAA TCCCTGACTC CAGAACGCAC TCAGCTCCTC
    TATCTCCTTG CTGTTGCTGT TGTCATCATT CTGTTTATTA TTCTGCTGGG GGTAATCATG
    GCAAAACGAA AGCGTAAGCA TGGCTCTCTC TGGCTGCCTG AAGGTTTCAC TCTTCGCCGA
    GATGCAAGCA ATCACAAGCG TCGTGAGCCA GTGGGACAGG ATGCTGTGGG GCTGAAAAAT
    CTCTCAGTGC AAGTCTCAGA AGCTAACCTA ATTGGTACTG GAACAAGTGA ACACTGGGTC
    GATGATGAAG GGCCCCAGCC AAAGAAAGTA AAGGCTGAAG ATGAGGCCTT ACTCTCAGAA
    GAAGATGACC CCATTGATCG ACGGCCATGG ACACAGCAGC ACCTTGAAGC TGCAGACATC
    CGTAGGACAC CATCGCTGGC TCTCACCCCT CCTCAGGCAG AGCAGGAGGT GGATGTGTTA
    GATGTGAATG TCCGTGGCCC AGATGGCTGC ACCCCATTGA TGTTGGCTTC TCTCCGAGGA
    GGCAGCTCAG ATTTGAGTGA TGAAGATGAA GATGCAGAGG ACTCTTCTGC TAACATCATC
    ACAGACTTGG TCTACCAGGG TGCCAGCCTC CAGGCCCAGA CAGACCGGAC TGGTGAGATG
    GCCCTGCACC TTGCAGCCCG CTACTCACGG GCTGATGCTG CCAAGCGTCT CCTGGATGCA
    GGTGCAGATG CCAATGCCCA GGACAACATG GGCCGCTGTC CACTCCATGC TGCAGTGGCA
    GCTGATGCCC AAGGTGTCTT CCAGATTCTG ATTCGCAACC GAGTAACTGA TCTAGATGCC
    AGGATGAATG ATGGTACTAC ACCCCTGATC CTGGCTGCCC GCCTGGCTGT GGAGGGAATG
    GTGGCAGAAC TGATCAACTG CCAAGCGGAT GTGAATGCAG TGGATGACCA TGGAAAATCT
    GCTCTTCACT GGGCAGCTGC TGTCAATAAT GTGGAGGCAA CTCTTTTGTT GTTGAAAAAT
    GGGGCCAACC GAGACATGCA GGACAACAAG GAAGAGACAC CTCTGTTTCT TGCTGCCCGG
    GAGGGGAGCT ATGAAGCAGC CAAGATCCTG TTAGACCATT TTGCCAATCG AGACATCACA
    GACCATATGG ATCGTCTTCC CCGGGATGTG GCTCGGGATC GCATGCACCA TGACATTGTG
    CGCCTTCTGG ATGAATACAA TGTGACCCCA AGCCCTCCAG GCACCGTGTT GACTTCTGCT
    CTCTCACCTG TCATCTGTGG GCCCAACAGA TCTTTCCTCA GCCTGAAGCA CACCCCAATG
    GGCAAGAAGT CTAGACGGCC CAGTGCCAAG AGTACCATGC CTACTAGCCT CCCTAACCTT
    GCCAAGGAGG CAAAGGATGC CAAGGGTAGT AGGAGGAAGA AGTCTCTGAG TGAGAAGGTC
    CAACTGTCTG AGAGTTCAGT AACTTTATCC CCTGTTGATT CCCTAGAATC TCCTCACACG
    TATGTTTCCG ACACCACATC CTCTCCAATG ATTACATCCC CTGGGATCTT ACAGGCCTCA
    CCCAACCCTA TGTTGGCCAC TGCCGCCCCT CCTGCCCCAG TCCATGCCCA GCATGCACTA
    TCTTTTTCTA ACCTTCATGA AATGCAGCCT TTGGCACATG GGGCCAGCAC TGTGCTTCCC
    TCAGTGAGCC AGTTGCTATC CCACCACCAC ATTGTGTCTC CAGGCAGTGG CAGTGCTGGA
    AGCTTGAGTA GGCTCCATCC AGTCCCAGTC CCAGCAGATT GGATGAACCG CATGGAGGTG
    AATGAGACCC AGTACAATGA GATGTTTGGT ATGGTCCTGG CTCCAGCTGA GGGCACCCAT
    CCTGGCATAG CTCCCCAGAG CAGGCCACCT GAAGGGAAGC ACATAACCAC CCCTCGGGAG
    CCCTTGCCCC CCATTGTGAC TTTCCAGCTC ATCCCTAAAG GCAGTATTGC CCAACCAGCG
    GGGGCTCCCC AGCCTCAGTC CACCTGCCCT CCAGCTGTTG CGGGCCCCCT GCCCACCATG
    TACCAGATTC CAGAAATGGC CCGTTTGCCC AGTGTGGCTT TCCCCACTGC CATGATGCCC
    CAGCAGGACG GGCAGGTAGC TCAGACCATT CTCCCAGCCT ATCATCCTTT CCCAGCCTCT
    GTGGGCAAGT ACCCCACACC CCCTTCACAG CACAGTTATG CTTCCTCAAA TGCTGCTGAG
    CGAACACCCA GTCACAGTGG TCACCTCCAG GGTGAGCATC CCTACCTGAC ACCATCCCCA
    GAGTCTCCTG ACCAGTGGTC AAGTTCATCA CCCCACTCTG CTTCTGACTG GTCAGATGTG
    ACCACCAGCC CTACCCCTGG GGGTGCTGGA GGAGGTCAGC GGGGACCTGG GACACACATG
    TCTGAGCCAC CACACAACAA CATGCAGGTT TATGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu90064
    Clone ID Related Accession (Same CDS sequence) XM_017001372.1
    Accession Version XM_017001372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurogenic locus notch homolog protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTGCTTCA CATTGTTGCA CAAAGCATTG CAGTGTCGAG ATGGCTATGA ACCCTGTGTA 
    AATGAAGGAA TGTGTGTTAC CTACCACAAT GGCACAGGAT ACTGCAAATG TCCAGAAGGC
    TTCTTGGGGG AATATTGTCA ACATCGAGAC CCCTGTGAGA AGAACCGCTG CCAGAATGGT
    GGGACTTGTG TGGCCCAGGC CATGCTGGGG AAAGCCACGT GCCGATGTGC CTCAGGGTTT
    ACAGGAGAGG ACTGCCAGTA CTCAACATCT CATCCATGCT TTGTGTCTCG ACCCTGCCTG
    AATGGCGGCA CATGCCATAT GCTCAGCCGG GATACCTATG AGTGCACCTG TCAAGTCGGG
    TTTACAGGTA AGGAGTGCCA ATGGACGGAT GCCTGCCTGT CTCATCCCTG TGCAAATGGA
    AGTACCTGTA CCACTGTGGC CAACCAGTTC TCCTGCAAAT GCCTCACAGG CTTCACAGGG
    CAGAAATGTG AGACTGATGT CAATGAGTGT GACATTCCAG GACACTGCCA GCATGGTGGC
    ACCTGCCTCA ACCTGCCTGG TTCCTACCAG TGCCAGTGCC CTCAGGGCTT CACAGGCCAG
    TACTGTGACA GCCTGTATGT GCCCTGTGCA CCCTCACCTT GTGTCAATGG AGGCACCTGT
    CGGCAGACTG GTGACTTCAC TTTTGAGTGC AACTGCCTTC CAGGTTTTGA AGGGAGCACC
    TGTGAGAGGA ATATTGATGA CTGCCCTAAC CACAGGTGTC AGAATGGAGG GGTTTGTGTG
    GATGGGGTCA ACACTTACAA CTGCCGCTGT CCCCCACAAT GGACAGGACA GTTCTGCACA
    GAGGATGTGG ATGAATGCCT GCTGCAGCCC AATGCCTGTC AAAATGGGGG CACCTGTGCC
    AACCGCAATG GAGGCTATGG CTGTGTATGT GTCAACGGCT GGAGTGGAGA TGACTGCAGT
    GAGAACATTG ATGATTGTGC CTTCGCCTCC TGTACTCCAG GCTCCACCTG CATCGACCGT
    GTGGCCTCCT TCTCTTGCAT GTGCCCAGAG GGGAAGGCAG GTCTCCTGTG TCATCTGGAT
    GATGCATGCA TCAGCAATCC TTGCCACAAG GGGGCACTGT GTGACACCAA CCCCCTAAAT
    GGGCAATATA TTTGCACCTG CCCACAAGGC TACAAAGGGG CTGACTGCAC AGAAGATGTG
    GATGAATGTG CCATGGCCAA TAGCAATCCT TGTGAGCATG CAGGAAAATG TGTGAACACG
    GATGGCGCCT TCCACTGTGA GTGTCTGAAG GGTTATGCAG GACCTCGTTG TGAGATGGAC
    ATCAATGAGT GCCATTCAGA CCCCTGCCAG AATGATGCTA CCTGTCTGGA TAAGATTGGA
    GGCTTCACAT GTCTGTGCAT GCCAGGTTTC AAAGGTGTGC ATTGTGAATT AGAAATAAAT
    GAATGTCAGA GCAACCCTTG TGTGAACAAT GGGCAGTGTG TGGATAAAGT CAATCGTTTC
    CAGTGCCTGT GTCCTCCTGG TTTCACTGGG CCAGTTTGCC AGATTGATAT TGATGACTGT
    TCCAGTACTC CGTGTCTGAA TGGGGCAAAG TGTATCGATC ACCCGAATGG CTATGAATGC
    CAGTGTGCCA CAGGTTTCAC TGGTGTGTTG TGTGAGGAGA ACATTGACAA CTGTGACCCC
    GATCCTTGCC ACCATGGTCA GTGTCAGGAT GGTATTGATT CCTACACCTG CATCTGCAAT
    CCCGGGTACA TGGGCGCCAT CTGCAGTGAC CAGATTGATG AATGTTACAG CAGCCCTTGC
    CTGAACGATG GTCGCTGCAT TGACCTGGTC AATGGCTACC AGTGCAACTG CCAGCCAGGC
    ACGTCAGGGG TTAATTGTGA AATTAATTTT GATGACTGTG CAAGTAACCC TTGTATCCAT
    GGAATCTGTA TGGATGGCAT TAATCGCTAC AGTTGTGTCT GCTCACCAGG ATTCACAGGG
    CAGAGATGTA ACATTGACAT TGATGAGTGT GCCTCCAATC CCTGTCGCAA GGGTGCAACA
    TGTATCAACG GTGTGAATGG TTTCCGCTGT ATATGCCCCG AGGGACCCCA TCACCCCAGC
    TGCTACTCAC AGGTGAACGA ATGCCTGAGC AATCCCTGCA TCCATGGAAA CTGTACTGGA
    GGTCTCAGTG GATATAAGTG TCTCTGTGAT GCAGGCTGGG TTGGCATCAA CTGTGAAGTG
    GACAAAAATG AATGCCTTTC GAATCCATGC CAGAATGGAG GAACTTGTGA CAATCTGGTG
    AATGGATACA GGTGTACTTG CAAGAAGGGC TTTAAAGGCT ATAACTGCCA GGTGAATATT
    GATGAATGTG CCTCAAATCC ATGCCTGAAC CAAGGAACCT GCTTTGATGA CATAAGTGGC
    TACACTTGCC ACTGTGTGCT GCCATACACA GGCAAGAATT GTCAGACAGT ATTGGCTCCC
    TGTTCCCCAA ACCCTTGTGA GAATGCTGCT GTTTGCAAAG AGTCACCAAA TTTTGAGAGT
    TATACTTGCT TGTGTGCTCC TGGCTGGCAA GGTCAGCGGT GTACCATTGA CATTGACGAG
    TGTATCTCCA AGCCCTGCAT GAACCATGGT CTCTGCCATA ACACCCAGGG CAGCTACATG
    TGTGAATGTC CACCAGGCTT CAGTGGTATG GACTGTGAGG AGGACATTGA TGACTGCCTT
    GCCAATCCTT GCCAGAATGG AGGTTCCTGT ATGGATGGAG TGAATACTTT CTCCTGCCTC
    TGCCTTCCGG GTTTCACTGG GGATAAGTGC CAGACAGACA TGAATGAGTG TCTGAGTGAA
    CCCTGTAAGA ATGGAGGGAC CTGCTCTGAC TACGTCAACA GTTACACTTG CAAGTGCCAG
    GCAGGATTTG ATGGAGTCCA TTGTGAGAAC AACATCAATG AGTGCACTGA GAGCTCCTGT
    TTCAATGGTG GCACATGTGT TGATGGGATT AACTCCTTCT CTTGCTTGTG CCCTGTGGGT
    TTCACTGGAT CCTTCTGCCT CCATGAGATC AATGAATGCA GCTCTCATCC ATGCCTGAAT
    GAGGGAACGT GTGTTGATGG CCTGGGTACC TACCGCTGCA GCTGCCCCCT GGGCTACACT
    GGGAAAAACT GTCAGACCCT GGTGAATCTC TGCAGTCGGT CTCCATGTAA AAACAAAGGT
    ACTTGCGTTC AGAAAAAAGC AGAGTCCCAG TGCCTATGTC CATCTGGATG GGCTGGTGCC
    TATTGTGACG TGCCCAATGT CTCTTGTGAC ATAGCAGCCT CCAGGAGAGG TGTGCTTGTT
    GAACACTTGT GCCAGCACTC AGGTGTCTGC ATCAATGCTG GCAACACGCA TTACTGTCAG
    TGCCCCCTGG GCTATACTGG GAGCTACTGT GAGGAGCAAC TCGATGAGTG TGCGTCCAAC
    CCCTGCCAGC ACGGGGCAAC ATGCAGTGAC TTCATTGGTG GATACAGATG CGAGTGTGTC
    CCAGGCTATC AGGGTGTCAA CTGTGAGTAT GAAGTGGATG AGTGCCAGAA TCAGCCCTGC
    CAGAATGGAG GCACCTGTAT TGACCTTGTG AACCATTTCA AGTGCTCTTG CCCACCAGGC
    ACTCGGGGCC TACTCTGTGA AGAGAACATT GATGACTGTG CCCGGGGTCC CCATTGCCTT
    AATGGTGGTC AGTGCATGGA TAGGATTGGA GGCTACAGTT GTCGCTGCTT GCCTGGCTTT
    GCTGGGGAGC GTTGTGAGGG AGACATCAAC GAGTGCCTCT CCAACCCCTG CAGCTCTGAG
    GGCAGCCTGG ACTGTATACA GCTCACCAAT GACTACCTGT GTGTTTGCCG TAGTGCCTTT
    ACTGGCCGGC ACTGTGAAAC CTTCGTCGAT GTGTGTCCCC AGATGCCCTG CCTGAATGGA
    GGGACTTGTG CTGTGGCCAG TAACATGCCT GATGGTTTCA TTTGCCGTTG TCCCCCGGGA
    TTTTCCGGGG CAAGGTGCCA GAGCAGCTGT GGACAAGTGA AATGTAGGAA GGGGGAGCAG
    TGTGTGCACA CCGCCTCTGG ACCCCGCTGC TTCTGCCCCA GTCCCCGGGA CTGCGAGTCA
    GGCTGTGCCA GTAGCCCCTG CCAGCACGGG GGCAGCTGCC ACCCTCAGCG CCAGCCTCCT
    TATTACTCCT GCCAGTGTGC CCCACCATTC TCGGGTAGCC GCTGTGAACT CTACACGGCA
    CCCCCCAGCA CCCCTCCTGC CACCTGTCTG AGCCAGTATT GTGCCGACAA AGCTCGGGAT
    GGCGTCTGTG ATGAGGCCTG CAACAGCCAT GCCTGCCAGT GGGATGGGGG TGACTGTTCT
    CTCACCATGG AGAACCCCTG GGCCAACTGC TCCTCCCCAC TTCCCTGCTG GGATTATATC
    AACAACCAGT GTGATGAGCT GTGCAACACG GTCGAGTGCC TGTTTGACAA CTTTGAATGC
    CAGGGGAACA GCAAGACATG CAAGTATGAC AAATACTGTG CAGACCACTT CAAAGACAAC
    CACTGTGACC AGGGGTGCAA CAGTGAGGAG TGTGGTTGGG ATGGGCTGGA CTGTGCTGCT
    GACCAACCTG AGAACCTGGC AGAAGGTACC CTGGTTATTG TGGTATTGAT GCCACCTGAA
    CAACTGCTCC AGGATGCTCG CAGCTTCTTG CGGGCACTGG GTACCCTGCT CCACACCAAC
    CTGCGCATTA AGCGGGACTC CCAGGGGGAA CTCATGGTGT ACCCCTATTA TGGTGAGAAG
    TCAGCTGCTA TGAAGAAACA GAGGATGACA CGCAGATCCC TTCCTGGTGA ACAAGAACAG
    GAGGTGGCTG GCTCTAAAGT CTTTCTGGAA ATTGACAACC GCCAGTGTGT TCAAGACTCA
    GACCACTGCT TCAAGAACAC GGATGCAGCA GCAGCTCTCC TGGCCTCTCA CGCCATACAG
    GGGACCCTGT CATACCCTCT TGTGTCTGTC GTCAGTGAAT CCCTGACTCC AGAACGCACT
    CAGCTCCTCT ATCTCCTTGC TGTTGCTGTT GTCATCATTC TGTTTATTAT TCTGCTGGGG
    GTAATCATGG CAAAACGAAA GCGTAAGCAT GGCTCTCTCT GGCTGCCTGA AGGTTTCACT
    CTTCGCCGAG ATGCAAGCAA TCACAAGCGT CGTGAGCCAG TGGGACAGGA TGCTGTGGGG
    CTGAAAAATC TCTCAGTGCA AGTCTCAGAA GCTAACCTAA TTGGTACTGG AACAAGTGAA
    CACTGGGTCG ATGATGAAGG GCCCCAGCCA AAGAAAGTAA AGGCTGAAGA TGAGGCCTTA
    CTCTCAGAAG AAGATGACCC CATTGATCGA CGGCCATGGA CACAGCAGCA CCTTGAAGCT
    GCAGACATCC GTAGGACACC ATCGCTGGCT CTCACCCCTC CTCAGGCAGA GCAGGAGGTG
    GATGTGTTAG ATGTGAATGT CCGTGGCCCA GATGGCTGCA CCCCATTGAT GTTGGCTTCT
    CTCCGAGGAG GCAGCTCAGA TTTGAGTGAT GAAGATGAAG ATGCAGAGGA CTCTTCTGCT
    AACATCATCA CAGACTTGGT CTACCAGGGT GCCAGCCTCC AGGCCCAGAC AGACCGGACT
    GGTGAGATGG CCCTGCACCT TGCAGCCCGC TACTCACGGG CTGATGCTGC CAAGCGTCTC
    CTGGATGCAG GTGCAGATGC CAATGCCCAG GACAACATGG GCCGCTGTCC ACTCCATGCT
    GCAGTGGCAG CTGATGCCCA AGGTGTCTTC CAGATTCTGA TTCGCAACCG AGTAACTGAT
    CTAGATGCCA GGATGAATGA TGGTACTACA CCCCTGATCC TGGCTGCCCG CCTGGCTGTG
    GAGGGAATGG TGGCAGAACT GATCAACTGC CAAGCGGATG TGAATGCAGT GGATGACCAT
    GGAAAATCTG CTCTTCACTG GGCAGCTGCT GTCAATAATG TGGAGGCAAC TCTTTTGTTG
    TTGAAAAATG GGGCCAACCG AGACATGCAG GACAACAAGG AAGAGACACC TCTGTTTCTT
    GCTGCCCGGG AGGGGAGCTA TGAAGCAGCC AAGATCCTGT TAGACCATTT TGCCAATCGA
    GACATCACAG ACCATATGGA TCGTCTTCCC CGGGATGTGG CTCGGGATCG CATGCACCAT
    GACATTGTGC GCCTTCTGGA TGAATACAAT GTGACCCCAA GCCCTCCAGG CACCGTGTTG
    ACTTCTGCTC TCTCACCTGT CATCTGTGGG CCCAACAGAT CTTTCCTCAG CCTGAAGCAC
    ACCCCAATGG GCAAGAAGTC TAGACGGCCC AGTGCCAAGA GTACCATGCC TACTAGCCTC
    CCTAACCTTG CCAAGGAGGC AAAGGATGCC AAGGGTAGTA GGAGGAAGAA GTCTCTGAGT
    GAGAAGGTCC AACTGTCTGA GAGTTCAGTA ACTTTATCCC CTGTTGATTC CCTAGAATCT
    CCTCACACGT ATGTTTCCGA CACCACATCC TCTCCAATGA TTACATCCCC TGGGATCTTA
    CAGGCCTCAC CCAACCCTAT GTTGGCCACT GCCGCCCCTC CTGCCCCAGT CCATGCCCAG
    CATGCACTAT CTTTTTCTAA CCTTCATGAA ATGCAGCCTT TGGCACATGG GGCCAGCACT
    GTGCTTCCCT CAGTGAGCCA GTTGCTATCC CACCACCACA TTGTGTCTCC AGGCAGTGGC
    AGTGCTGGAA GCTTGAGTAG GCTCCATCCA GTCCCAGTCC CAGCAGATTG GATGAACCGC
    ATGGAGGTGA ATGAGACCCA GTACAATGAG ATGTTTGGTA TGGTCCTGGC TCCAGCTGAG
    GGCACCCATC CTGGCATAGC TCCCCAGAGC AGGCCACCTG AAGGGAAGCA CATAACCACC
    CCTCGGGAGC CCTTGCCCCC CATTGTGACT TTCCAGCTCA TCCCTAAAGG CAGTATTGCC
    CAACCAGCGG GGGCTCCCCA GCCTCAGTCC ACCTGCCCTC CAGCTGTTGC GGGCCCCCTG
    CCCACCATGT ACCAGATTCC AGAAATGGCC CGTTTGCCCA GTGTGGCTTT CCCCACTGCC
    ATGATGCCCC AGCAGGACGG GCAGGTAGCT CAGACCATTC TCCCAGCCTA TCATCCTTTC
    CCAGCCTCTG TGGGCAAGTA CCCCACACCC CCTTCACAGC ACAGTTATGC TTCCTCAAAT
    GCTGCTGAGC GAACACCCAG TCACAGTGGT CACCTCCAGG GTGAGCATCC CTACCTGACA
    CCATCCCCAG AGTCTCCTGA CCAGTGGTCA AGTTCATCAC CCCACTCTGC TTCTGACTGG
    TCAGATGTGA CCACCAGCCC TACCCCTGGG GGTGCTGGAG GAGGTCAGCG GGGACCTGGG
    ACACACATGT CTGAGCCACC ACACAACAAC ATGCAGGTTT ATGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016856861.1
    CDS16..7383
    Translation

    Target ORF information:

    RefSeq Version XM_017001372.1
    Organism Homo sapiens(human)
    Definition Homo sapiens notch 2 (NOTCH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017001372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGTGCTTCA CATTGTTGCA CAAAGCATTG CAGTGTCGAG ATGGCTATGA ACCCTGTGTA 
    AATGAAGGAA TGTGTGTTAC CTACCACAAT GGCACAGGAT ACTGCAAATG TCCAGAAGGC
    TTCTTGGGGG AATATTGTCA ACATCGAGAC CCCTGTGAGA AGAACCGCTG CCAGAATGGT
    GGGACTTGTG TGGCCCAGGC CATGCTGGGG AAAGCCACGT GCCGATGTGC CTCAGGGTTT
    ACAGGAGAGG ACTGCCAGTA CTCAACATCT CATCCATGCT TTGTGTCTCG ACCCTGCCTG
    AATGGCGGCA CATGCCATAT GCTCAGCCGG GATACCTATG AGTGCACCTG TCAAGTCGGG
    TTTACAGGTA AGGAGTGCCA ATGGACGGAT GCCTGCCTGT CTCATCCCTG TGCAAATGGA
    AGTACCTGTA CCACTGTGGC CAACCAGTTC TCCTGCAAAT GCCTCACAGG CTTCACAGGG
    CAGAAATGTG AGACTGATGT CAATGAGTGT GACATTCCAG GACACTGCCA GCATGGTGGC
    ACCTGCCTCA ACCTGCCTGG TTCCTACCAG TGCCAGTGCC CTCAGGGCTT CACAGGCCAG
    TACTGTGACA GCCTGTATGT GCCCTGTGCA CCCTCACCTT GTGTCAATGG AGGCACCTGT
    CGGCAGACTG GTGACTTCAC TTTTGAGTGC AACTGCCTTC CAGGTTTTGA AGGGAGCACC
    TGTGAGAGGA ATATTGATGA CTGCCCTAAC CACAGGTGTC AGAATGGAGG GGTTTGTGTG
    GATGGGGTCA ACACTTACAA CTGCCGCTGT CCCCCACAAT GGACAGGACA GTTCTGCACA
    GAGGATGTGG ATGAATGCCT GCTGCAGCCC AATGCCTGTC AAAATGGGGG CACCTGTGCC
    AACCGCAATG GAGGCTATGG CTGTGTATGT GTCAACGGCT GGAGTGGAGA TGACTGCAGT
    GAGAACATTG ATGATTGTGC CTTCGCCTCC TGTACTCCAG GCTCCACCTG CATCGACCGT
    GTGGCCTCCT TCTCTTGCAT GTGCCCAGAG GGGAAGGCAG GTCTCCTGTG TCATCTGGAT
    GATGCATGCA TCAGCAATCC TTGCCACAAG GGGGCACTGT GTGACACCAA CCCCCTAAAT
    GGGCAATATA TTTGCACCTG CCCACAAGGC TACAAAGGGG CTGACTGCAC AGAAGATGTG
    GATGAATGTG CCATGGCCAA TAGCAATCCT TGTGAGCATG CAGGAAAATG TGTGAACACG
    GATGGCGCCT TCCACTGTGA GTGTCTGAAG GGTTATGCAG GACCTCGTTG TGAGATGGAC
    ATCAATGAGT GCCATTCAGA CCCCTGCCAG AATGATGCTA CCTGTCTGGA TAAGATTGGA
    GGCTTCACAT GTCTGTGCAT GCCAGGTTTC AAAGGTGTGC ATTGTGAATT AGAAATAAAT
    GAATGTCAGA GCAACCCTTG TGTGAACAAT GGGCAGTGTG TGGATAAAGT CAATCGTTTC
    CAGTGCCTGT GTCCTCCTGG TTTCACTGGG CCAGTTTGCC AGATTGATAT TGATGACTGT
    TCCAGTACTC CGTGTCTGAA TGGGGCAAAG TGTATCGATC ACCCGAATGG CTATGAATGC
    CAGTGTGCCA CAGGTTTCAC TGGTGTGTTG TGTGAGGAGA ACATTGACAA CTGTGACCCC
    GATCCTTGCC ACCATGGTCA GTGTCAGGAT GGTATTGATT CCTACACCTG CATCTGCAAT
    CCCGGGTACA TGGGCGCCAT CTGCAGTGAC CAGATTGATG AATGTTACAG CAGCCCTTGC
    CTGAACGATG GTCGCTGCAT TGACCTGGTC AATGGCTACC AGTGCAACTG CCAGCCAGGC
    ACGTCAGGGG TTAATTGTGA AATTAATTTT GATGACTGTG CAAGTAACCC TTGTATCCAT
    GGAATCTGTA TGGATGGCAT TAATCGCTAC AGTTGTGTCT GCTCACCAGG ATTCACAGGG
    CAGAGATGTA ACATTGACAT TGATGAGTGT GCCTCCAATC CCTGTCGCAA GGGTGCAACA
    TGTATCAACG GTGTGAATGG TTTCCGCTGT ATATGCCCCG AGGGACCCCA TCACCCCAGC
    TGCTACTCAC AGGTGAACGA ATGCCTGAGC AATCCCTGCA TCCATGGAAA CTGTACTGGA
    GGTCTCAGTG GATATAAGTG TCTCTGTGAT GCAGGCTGGG TTGGCATCAA CTGTGAAGTG
    GACAAAAATG AATGCCTTTC GAATCCATGC CAGAATGGAG GAACTTGTGA CAATCTGGTG
    AATGGATACA GGTGTACTTG CAAGAAGGGC TTTAAAGGCT ATAACTGCCA GGTGAATATT
    GATGAATGTG CCTCAAATCC ATGCCTGAAC CAAGGAACCT GCTTTGATGA CATAAGTGGC
    TACACTTGCC ACTGTGTGCT GCCATACACA GGCAAGAATT GTCAGACAGT ATTGGCTCCC
    TGTTCCCCAA ACCCTTGTGA GAATGCTGCT GTTTGCAAAG AGTCACCAAA TTTTGAGAGT
    TATACTTGCT TGTGTGCTCC TGGCTGGCAA GGTCAGCGGT GTACCATTGA CATTGACGAG
    TGTATCTCCA AGCCCTGCAT GAACCATGGT CTCTGCCATA ACACCCAGGG CAGCTACATG
    TGTGAATGTC CACCAGGCTT CAGTGGTATG GACTGTGAGG AGGACATTGA TGACTGCCTT
    GCCAATCCTT GCCAGAATGG AGGTTCCTGT ATGGATGGAG TGAATACTTT CTCCTGCCTC
    TGCCTTCCGG GTTTCACTGG GGATAAGTGC CAGACAGACA TGAATGAGTG TCTGAGTGAA
    CCCTGTAAGA ATGGAGGGAC CTGCTCTGAC TACGTCAACA GTTACACTTG CAAGTGCCAG
    GCAGGATTTG ATGGAGTCCA TTGTGAGAAC AACATCAATG AGTGCACTGA GAGCTCCTGT
    TTCAATGGTG GCACATGTGT TGATGGGATT AACTCCTTCT CTTGCTTGTG CCCTGTGGGT
    TTCACTGGAT CCTTCTGCCT CCATGAGATC AATGAATGCA GCTCTCATCC ATGCCTGAAT
    GAGGGAACGT GTGTTGATGG CCTGGGTACC TACCGCTGCA GCTGCCCCCT GGGCTACACT
    GGGAAAAACT GTCAGACCCT GGTGAATCTC TGCAGTCGGT CTCCATGTAA AAACAAAGGT
    ACTTGCGTTC AGAAAAAAGC AGAGTCCCAG TGCCTATGTC CATCTGGATG GGCTGGTGCC
    TATTGTGACG TGCCCAATGT CTCTTGTGAC ATAGCAGCCT CCAGGAGAGG TGTGCTTGTT
    GAACACTTGT GCCAGCACTC AGGTGTCTGC ATCAATGCTG GCAACACGCA TTACTGTCAG
    TGCCCCCTGG GCTATACTGG GAGCTACTGT GAGGAGCAAC TCGATGAGTG TGCGTCCAAC
    CCCTGCCAGC ACGGGGCAAC ATGCAGTGAC TTCATTGGTG GATACAGATG CGAGTGTGTC
    CCAGGCTATC AGGGTGTCAA CTGTGAGTAT GAAGTGGATG AGTGCCAGAA TCAGCCCTGC
    CAGAATGGAG GCACCTGTAT TGACCTTGTG AACCATTTCA AGTGCTCTTG CCCACCAGGC
    ACTCGGGGCC TACTCTGTGA AGAGAACATT GATGACTGTG CCCGGGGTCC CCATTGCCTT
    AATGGTGGTC AGTGCATGGA TAGGATTGGA GGCTACAGTT GTCGCTGCTT GCCTGGCTTT
    GCTGGGGAGC GTTGTGAGGG AGACATCAAC GAGTGCCTCT CCAACCCCTG CAGCTCTGAG
    GGCAGCCTGG ACTGTATACA GCTCACCAAT GACTACCTGT GTGTTTGCCG TAGTGCCTTT
    ACTGGCCGGC ACTGTGAAAC CTTCGTCGAT GTGTGTCCCC AGATGCCCTG CCTGAATGGA
    GGGACTTGTG CTGTGGCCAG TAACATGCCT GATGGTTTCA TTTGCCGTTG TCCCCCGGGA
    TTTTCCGGGG CAAGGTGCCA GAGCAGCTGT GGACAAGTGA AATGTAGGAA GGGGGAGCAG
    TGTGTGCACA CCGCCTCTGG ACCCCGCTGC TTCTGCCCCA GTCCCCGGGA CTGCGAGTCA
    GGCTGTGCCA GTAGCCCCTG CCAGCACGGG GGCAGCTGCC ACCCTCAGCG CCAGCCTCCT
    TATTACTCCT GCCAGTGTGC CCCACCATTC TCGGGTAGCC GCTGTGAACT CTACACGGCA
    CCCCCCAGCA CCCCTCCTGC CACCTGTCTG AGCCAGTATT GTGCCGACAA AGCTCGGGAT
    GGCGTCTGTG ATGAGGCCTG CAACAGCCAT GCCTGCCAGT GGGATGGGGG TGACTGTTCT
    CTCACCATGG AGAACCCCTG GGCCAACTGC TCCTCCCCAC TTCCCTGCTG GGATTATATC
    AACAACCAGT GTGATGAGCT GTGCAACACG GTCGAGTGCC TGTTTGACAA CTTTGAATGC
    CAGGGGAACA GCAAGACATG CAAGTATGAC AAATACTGTG CAGACCACTT CAAAGACAAC
    CACTGTGACC AGGGGTGCAA CAGTGAGGAG TGTGGTTGGG ATGGGCTGGA CTGTGCTGCT
    GACCAACCTG AGAACCTGGC AGAAGGTACC CTGGTTATTG TGGTATTGAT GCCACCTGAA
    CAACTGCTCC AGGATGCTCG CAGCTTCTTG CGGGCACTGG GTACCCTGCT CCACACCAAC
    CTGCGCATTA AGCGGGACTC CCAGGGGGAA CTCATGGTGT ACCCCTATTA TGGTGAGAAG
    TCAGCTGCTA TGAAGAAACA GAGGATGACA CGCAGATCCC TTCCTGGTGA ACAAGAACAG
    GAGGTGGCTG GCTCTAAAGT CTTTCTGGAA ATTGACAACC GCCAGTGTGT TCAAGACTCA
    GACCACTGCT TCAAGAACAC GGATGCAGCA GCAGCTCTCC TGGCCTCTCA CGCCATACAG
    GGGACCCTGT CATACCCTCT TGTGTCTGTC GTCAGTGAAT CCCTGACTCC AGAACGCACT
    CAGCTCCTCT ATCTCCTTGC TGTTGCTGTT GTCATCATTC TGTTTATTAT TCTGCTGGGG
    GTAATCATGG CAAAACGAAA GCGTAAGCAT GGCTCTCTCT GGCTGCCTGA AGGTTTCACT
    CTTCGCCGAG ATGCAAGCAA TCACAAGCGT CGTGAGCCAG TGGGACAGGA TGCTGTGGGG
    CTGAAAAATC TCTCAGTGCA AGTCTCAGAA GCTAACCTAA TTGGTACTGG AACAAGTGAA
    CACTGGGTCG ATGATGAAGG GCCCCAGCCA AAGAAAGTAA AGGCTGAAGA TGAGGCCTTA
    CTCTCAGAAG AAGATGACCC CATTGATCGA CGGCCATGGA CACAGCAGCA CCTTGAAGCT
    GCAGACATCC GTAGGACACC ATCGCTGGCT CTCACCCCTC CTCAGGCAGA GCAGGAGGTG
    GATGTGTTAG ATGTGAATGT CCGTGGCCCA GATGGCTGCA CCCCATTGAT GTTGGCTTCT
    CTCCGAGGAG GCAGCTCAGA TTTGAGTGAT GAAGATGAAG ATGCAGAGGA CTCTTCTGCT
    AACATCATCA CAGACTTGGT CTACCAGGGT GCCAGCCTCC AGGCCCAGAC AGACCGGACT
    GGTGAGATGG CCCTGCACCT TGCAGCCCGC TACTCACGGG CTGATGCTGC CAAGCGTCTC
    CTGGATGCAG GTGCAGATGC CAATGCCCAG GACAACATGG GCCGCTGTCC ACTCCATGCT
    GCAGTGGCAG CTGATGCCCA AGGTGTCTTC CAGATTCTGA TTCGCAACCG AGTAACTGAT
    CTAGATGCCA GGATGAATGA TGGTACTACA CCCCTGATCC TGGCTGCCCG CCTGGCTGTG
    GAGGGAATGG TGGCAGAACT GATCAACTGC CAAGCGGATG TGAATGCAGT GGATGACCAT
    GGAAAATCTG CTCTTCACTG GGCAGCTGCT GTCAATAATG TGGAGGCAAC TCTTTTGTTG
    TTGAAAAATG GGGCCAACCG AGACATGCAG GACAACAAGG AAGAGACACC TCTGTTTCTT
    GCTGCCCGGG AGGGGAGCTA TGAAGCAGCC AAGATCCTGT TAGACCATTT TGCCAATCGA
    GACATCACAG ACCATATGGA TCGTCTTCCC CGGGATGTGG CTCGGGATCG CATGCACCAT
    GACATTGTGC GCCTTCTGGA TGAATACAAT GTGACCCCAA GCCCTCCAGG CACCGTGTTG
    ACTTCTGCTC TCTCACCTGT CATCTGTGGG CCCAACAGAT CTTTCCTCAG CCTGAAGCAC
    ACCCCAATGG GCAAGAAGTC TAGACGGCCC AGTGCCAAGA GTACCATGCC TACTAGCCTC
    CCTAACCTTG CCAAGGAGGC AAAGGATGCC AAGGGTAGTA GGAGGAAGAA GTCTCTGAGT
    GAGAAGGTCC AACTGTCTGA GAGTTCAGTA ACTTTATCCC CTGTTGATTC CCTAGAATCT
    CCTCACACGT ATGTTTCCGA CACCACATCC TCTCCAATGA TTACATCCCC TGGGATCTTA
    CAGGCCTCAC CCAACCCTAT GTTGGCCACT GCCGCCCCTC CTGCCCCAGT CCATGCCCAG
    CATGCACTAT CTTTTTCTAA CCTTCATGAA ATGCAGCCTT TGGCACATGG GGCCAGCACT
    GTGCTTCCCT CAGTGAGCCA GTTGCTATCC CACCACCACA TTGTGTCTCC AGGCAGTGGC
    AGTGCTGGAA GCTTGAGTAG GCTCCATCCA GTCCCAGTCC CAGCAGATTG GATGAACCGC
    ATGGAGGTGA ATGAGACCCA GTACAATGAG ATGTTTGGTA TGGTCCTGGC TCCAGCTGAG
    GGCACCCATC CTGGCATAGC TCCCCAGAGC AGGCCACCTG AAGGGAAGCA CATAACCACC
    CCTCGGGAGC CCTTGCCCCC CATTGTGACT TTCCAGCTCA TCCCTAAAGG CAGTATTGCC
    CAACCAGCGG GGGCTCCCCA GCCTCAGTCC ACCTGCCCTC CAGCTGTTGC GGGCCCCCTG
    CCCACCATGT ACCAGATTCC AGAAATGGCC CGTTTGCCCA GTGTGGCTTT CCCCACTGCC
    ATGATGCCCC AGCAGGACGG GCAGGTAGCT CAGACCATTC TCCCAGCCTA TCATCCTTTC
    CCAGCCTCTG TGGGCAAGTA CCCCACACCC CCTTCACAGC ACAGTTATGC TTCCTCAAAT
    GCTGCTGAGC GAACACCCAG TCACAGTGGT CACCTCCAGG GTGAGCATCC CTACCTGACA
    CCATCCCCAG AGTCTCCTGA CCAGTGGTCA AGTTCATCAC CCCACTCTGC TTCTGACTGG
    TCAGATGTGA CCACCAGCCC TACCCCTGGG GGTGCTGGAG GAGGTCAGCG GGGACCTGGG
    ACACACATGT CTGAGCCACC ACACAACAAC ATGCAGGTTT ATGCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu59298
    Clone ID Related Accession (Same CDS sequence) XM_011541519.2
    Accession Version XM_011541519.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product neurogenic locus notch homolog protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011541519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGAACACAT ATTTGGTGTC GGCAGTTTCC CTCAGATGTG TTAGTGTGGA GAGATATAAA 
    GCATTGCAGT GTCGAGATGG CTATGAACCC TGTGTAAATG AAGGAATGTG TGTTACCTAC
    CACAATGGCA CAGGATACTG CAAATGTCCA GAAGGCTTCT TGGGGGAATA TTGTCAACAT
    CGAGACCCCT GTGAGAAGAA CCGCTGCCAG AATGGTGGGA CTTGTGTGGC CCAGGCCATG
    CTGGGGAAAG CCACGTGCCG ATGTGCCTCA GGGTTTACAG GAGAGGACTG CCAGTACTCA
    ACATCTCATC CATGCTTTGT GTCTCGACCC TGCCTGAATG GCGGCACATG CCATATGCTC
    AGCCGGGATA CCTATGAGTG CACCTGTCAA GTCGGGTTTA CAGGTAAGGA GTGCCAATGG
    ACGGATGCCT GCCTGTCTCA TCCCTGTGCA AATGGAAGTA CCTGTACCAC TGTGGCCAAC
    CAGTTCTCCT GCAAATGCCT CACAGGCTTC ACAGGGCAGA AATGTGAGAC TGATGTCAAT
    GAGTGTGACA TTCCAGGACA CTGCCAGCAT GGTGGCACCT GCCTCAACCT GCCTGGTTCC
    TACCAGTGCC AGTGCCCTCA GGGCTTCACA GGCCAGTACT GTGACAGCCT GTATGTGCCC
    TGTGCACCCT CACCTTGTGT CAATGGAGGC ACCTGTCGGC AGACTGGTGA CTTCACTTTT
    GAGTGCAACT GCCTTCCAGG TTTTGAAGGG AGCACCTGTG AGAGGAATAT TGATGACTGC
    CCTAACCACA GGTGTCAGAA TGGAGGGGTT TGTGTGGATG GGGTCAACAC TTACAACTGC
    CGCTGTCCCC CACAATGGAC AGGACAGTTC TGCACAGAGG ATGTGGATGA ATGCCTGCTG
    CAGCCCAATG CCTGTCAAAA TGGGGGCACC TGTGCCAACC GCAATGGAGG CTATGGCTGT
    GTATGTGTCA ACGGCTGGAG TGGAGATGAC TGCAGTGAGA ACATTGATGA TTGTGCCTTC
    GCCTCCTGTA CTCCAGGCTC CACCTGCATC GACCGTGTGG CCTCCTTCTC TTGCATGTGC
    CCAGAGGGGA AGGCAGGTCT CCTGTGTCAT CTGGATGATG CATGCATCAG CAATCCTTGC
    CACAAGGGGG CACTGTGTGA CACCAACCCC CTAAATGGGC AATATATTTG CACCTGCCCA
    CAAGGCTACA AAGGGGCTGA CTGCACAGAA GATGTGGATG AATGTGCCAT GGCCAATAGC
    AATCCTTGTG AGCATGCAGG AAAATGTGTG AACACGGATG GCGCCTTCCA CTGTGAGTGT
    CTGAAGGGTT ATGCAGGACC TCGTTGTGAG ATGGACATCA ATGAGTGCCA TTCAGACCCC
    TGCCAGAATG ATGCTACCTG TCTGGATAAG ATTGGAGGCT TCACATGTCT GTGCATGCCA
    GGTTTCAAAG GTGTGCATTG TGAATTAGAA ATAAATGAAT GTCAGAGCAA CCCTTGTGTG
    AACAATGGGC AGTGTGTGGA TAAAGTCAAT CGTTTCCAGT GCCTGTGTCC TCCTGGTTTC
    ACTGGGCCAG TTTGCCAGAT TGATATTGAT GACTGTTCCA GTACTCCGTG TCTGAATGGG
    GCAAAGTGTA TCGATCACCC GAATGGCTAT GAATGCCAGT GTGCCACAGG TTTCACTGGT
    GTGTTGTGTG AGGAGAACAT TGACAACTGT GACCCCGATC CTTGCCACCA TGGTCAGTGT
    CAGGATGGTA TTGATTCCTA CACCTGCATC TGCAATCCCG GGTACATGGG CGCCATCTGC
    AGTGACCAGA TTGATGAATG TTACAGCAGC CCTTGCCTGA ACGATGGTCG CTGCATTGAC
    CTGGTCAATG GCTACCAGTG CAACTGCCAG CCAGGCACGT CAGGGGTTAA TTGTGAAATT
    AATTTTGATG ACTGTGCAAG TAACCCTTGT ATCCATGGAA TCTGTATGGA TGGCATTAAT
    CGCTACAGTT GTGTCTGCTC ACCAGGATTC ACAGGGCAGA GATGTAACAT TGACATTGAT
    GAGTGTGCCT CCAATCCCTG TCGCAAGGGT GCAACATGTA TCAACGGTGT GAATGGTTTC
    CGCTGTATAT GCCCCGAGGG ACCCCATCAC CCCAGCTGCT ACTCACAGGT GAACGAATGC
    CTGAGCAATC CCTGCATCCA TGGAAACTGT ACTGGAGGTC TCAGTGGATA TAAGTGTCTC
    TGTGATGCAG GCTGGGTTGG CATCAACTGT GAAGTGGACA AAAATGAATG CCTTTCGAAT
    CCATGCCAGA ATGGAGGAAC TTGTGACAAT CTGGTGAATG GATACAGGTG TACTTGCAAG
    AAGGGCTTTA AAGGCTATAA CTGCCAGGTG AATATTGATG AATGTGCCTC AAATCCATGC
    CTGAACCAAG GAACCTGCTT TGATGACATA AGTGGCTACA CTTGCCACTG TGTGCTGCCA
    TACACAGGCA AGAATTGTCA GACAGTATTG GCTCCCTGTT CCCCAAACCC TTGTGAGAAT
    GCTGCTGTTT GCAAAGAGTC ACCAAATTTT GAGAGTTATA CTTGCTTGTG TGCTCCTGGC
    TGGCAAGGTC AGCGGTGTAC CATTGACATT GACGAGTGTA TCTCCAAGCC CTGCATGAAC
    CATGGTCTCT GCCATAACAC CCAGGGCAGC TACATGTGTG AATGTCCACC AGGCTTCAGT
    GGTATGGACT GTGAGGAGGA CATTGATGAC TGCCTTGCCA ATCCTTGCCA GAATGGAGGT
    TCCTGTATGG ATGGAGTGAA TACTTTCTCC TGCCTCTGCC TTCCGGGTTT CACTGGGGAT
    AAGTGCCAGA CAGACATGAA TGAGTGTCTG AGTGAACCCT GTAAGAATGG AGGGACCTGC
    TCTGACTACG TCAACAGTTA CACTTGCAAG TGCCAGGCAG GATTTGATGG AGTCCATTGT
    GAGAACAACA TCAATGAGTG CACTGAGAGC TCCTGTTTCA ATGGTGGCAC ATGTGTTGAT
    GGGATTAACT CCTTCTCTTG CTTGTGCCCT GTGGGTTTCA CTGGATCCTT CTGCCTCCAT
    GAGATCAATG AATGCAGCTC TCATCCATGC CTGAATGAGG GAACGTGTGT TGATGGCCTG
    GGTACCTACC GCTGCAGCTG CCCCCTGGGC TACACTGGGA AAAACTGTCA GACCCTGGTG
    AATCTCTGCA GTCGGTCTCC ATGTAAAAAC AAAGGTACTT GCGTTCAGAA AAAAGCAGAG
    TCCCAGTGCC TATGTCCATC TGGATGGGCT GGTGCCTATT GTGACGTGCC CAATGTCTCT
    TGTGACATAG CAGCCTCCAG GAGAGGTGTG CTTGTTGAAC ACTTGTGCCA GCACTCAGGT
    GTCTGCATCA ATGCTGGCAA CACGCATTAC TGTCAGTGCC CCCTGGGCTA TACTGGGAGC
    TACTGTGAGG AGCAACTCGA TGAGTGTGCG TCCAACCCCT GCCAGCACGG GGCAACATGC
    AGTGACTTCA TTGGTGGATA CAGATGCGAG TGTGTCCCAG GCTATCAGGG TGTCAACTGT
    GAGTATGAAG TGGATGAGTG CCAGAATCAG CCCTGCCAGA ATGGAGGCAC CTGTATTGAC
    CTTGTGAACC ATTTCAAGTG CTCTTGCCCA CCAGGCACTC GGGGCCTACT CTGTGAAGAG
    AACATTGATG ACTGTGCCCG GGGTCCCCAT TGCCTTAATG GTGGTCAGTG CATGGATAGG
    ATTGGAGGCT ACAGTTGTCG CTGCTTGCCT GGCTTTGCTG GGGAGCGTTG TGAGGGAGAC
    ATCAACGAGT GCCTCTCCAA CCCCTGCAGC TCTGAGGGCA GCCTGGACTG TATACAGCTC
    ACCAATGACT ACCTGTGTGT TTGCCGTAGT GCCTTTACTG GCCGGCACTG TGAAACCTTC
    GTCGATGTGT GTCCCCAGAT GCCCTGCCTG AATGGAGGGA CTTGTGCTGT GGCCAGTAAC
    ATGCCTGATG GTTTCATTTG CCGTTGTCCC CCGGGATTTT CCGGGGCAAG GTGCCAGAGC
    AGCTGTGGAC AAGTGAAATG TAGGAAGGGG GAGCAGTGTG TGCACACCGC CTCTGGACCC
    CGCTGCTTCT GCCCCAGTCC CCGGGACTGC GAGTCAGGCT GTGCCAGTAG CCCCTGCCAG
    CACGGGGGCA GCTGCCACCC TCAGCGCCAG CCTCCTTATT ACTCCTGCCA GTGTGCCCCA
    CCATTCTCGG GTAGCCGCTG TGAACTCTAC ACGGCACCCC CCAGCACCCC TCCTGCCACC
    TGTCTGAGCC AGTATTGTGC CGACAAAGCT CGGGATGGCG TCTGTGATGA GGCCTGCAAC
    AGCCATGCCT GCCAGTGGGA TGGGGGTGAC TGTTCTCTCA CCATGGAGAA CCCCTGGGCC
    AACTGCTCCT CCCCACTTCC CTGCTGGGAT TATATCAACA ACCAGTGTGA TGAGCTGTGC
    AACACGGTCG AGTGCCTGTT TGACAACTTT GAATGCCAGG GGAACAGCAA GACATGCAAG
    TATGACAAAT ACTGTGCAGA CCACTTCAAA GACAACCACT GTGACCAGGG GTGCAACAGT
    GAGGAGTGTG GTTGGGATGG GCTGGACTGT GCTGCTGACC AACCTGAGAA CCTGGCAGAA
    GGTACCCTGG TTATTGTGGT ATTGATGCCA CCTGAACAAC TGCTCCAGGA TGCTCGCAGC
    TTCTTGCGGG CACTGGGTAC CCTGCTCCAC ACCAACCTGC GCATTAAGCG GGACTCCCAG
    GGGGAACTCA TGGTGTACCC CTATTATGGT GAGAAGTCAG CTGCTATGAA GAAACAGAGG
    ATGACACGCA GATCCCTTCC TGGTGAACAA GAACAGGAGG TGGCTGGCTC TAAAGTCTTT
    CTGGAAATTG ACAACCGCCA GTGTGTTCAA GACTCAGACC ACTGCTTCAA GAACACGGAT
    GCAGCAGCAG CTCTCCTGGC CTCTCACGCC ATACAGGGGA CCCTGTCATA CCCTCTTGTG
    TCTGTCGTCA GTGAATCCCT GACTCCAGAA CGCACTCAGC TCCTCTATCT CCTTGCTGTT
    GCTGTTGTCA TCATTCTGTT TATTATTCTG CTGGGGGTAA TCATGGCAAA ACGAAAGCGT
    AAGCATGGCT CTCTCTGGCT GCCTGAAGGT TTCACTCTTC GCCGAGATGC AAGCAATCAC
    AAGCGTCGTG AGCCAGTGGG ACAGGATGCT GTGGGGCTGA AAAATCTCTC AGTGCAAGTC
    TCAGAAGCTA ACCTAATTGG TACTGGAACA AGTGAACACT GGGTCGATGA TGAAGGGCCC
    CAGCCAAAGA AAGTAAAGGC TGAAGATGAG GCCTTACTCT CAGAAGAAGA TGACCCCATT
    GATCGACGGC CATGGACACA GCAGCACCTT GAAGCTGCAG ACATCCGTAG GACACCATCG
    CTGGCTCTCA CCCCTCCTCA GGCAGAGCAG GAGGTGGATG TGTTAGATGT GAATGTCCGT
    GGCCCAGATG GCTGCACCCC ATTGATGTTG GCTTCTCTCC GAGGAGGCAG CTCAGATTTG
    AGTGATGAAG ATGAAGATGC AGAGGACTCT TCTGCTAACA TCATCACAGA CTTGGTCTAC
    CAGGGTGCCA GCCTCCAGGC CCAGACAGAC CGGACTGGTG AGATGGCCCT GCACCTTGCA
    GCCCGCTACT CACGGGCTGA TGCTGCCAAG CGTCTCCTGG ATGCAGGTGC AGATGCCAAT
    GCCCAGGACA ACATGGGCCG CTGTCCACTC CATGCTGCAG TGGCAGCTGA TGCCCAAGGT
    GTCTTCCAGA TTCTGATTCG CAACCGAGTA ACTGATCTAG ATGCCAGGAT GAATGATGGT
    ACTACACCCC TGATCCTGGC TGCCCGCCTG GCTGTGGAGG GAATGGTGGC AGAACTGATC
    AACTGCCAAG CGGATGTGAA TGCAGTGGAT GACCATGGAA AATCTGCTCT TCACTGGGCA
    GCTGCTGTCA ATAATGTGGA GGCAACTCTT TTGTTGTTGA AAAATGGGGC CAACCGAGAC
    ATGCAGGACA ACAAGGAAGA GACACCTCTG TTTCTTGCTG CCCGGGAGGG GAGCTATGAA
    GCAGCCAAGA TCCTGTTAGA CCATTTTGCC AATCGAGACA TCACAGACCA TATGGATCGT
    CTTCCCCGGG ATGTGGCTCG GGATCGCATG CACCATGACA TTGTGCGCCT TCTGGATGAA
    TACAATGTGA CCCCAAGCCC TCCAGGCACC GTGTTGACTT CTGCTCTCTC ACCTGTCATC
    TGTGGGCCCA ACAGATCTTT CCTCAGCCTG AAGCACACCC CAATGGGCAA GAAGTCTAGA
    CGGCCCAGTG CCAAGAGTAC CATGCCTACT AGCCTCCCTA ACCTTGCCAA GGAGGCAAAG
    GATGCCAAGG GTAGTAGGAG GAAGAAGTCT CTGAGTGAGA AGGTCCAACT GTCTGAGAGT
    TCAGTAACTT TATCCCCTGT TGATTCCCTA GAATCTCCTC ACACGTATGT TTCCGACACC
    ACATCCTCTC CAATGATTAC ATCCCCTGGG ATCTTACAGG CCTCACCCAA CCCTATGTTG
    GCCACTGCCG CCCCTCCTGC CCCAGTCCAT GCCCAGCATG CACTATCTTT TTCTAACCTT
    CATGAAATGC AGCCTTTGGC ACATGGGGCC AGCACTGTGC TTCCCTCAGT GAGCCAGTTG
    CTATCCCACC ACCACATTGT GTCTCCAGGC AGTGGCAGTG CTGGAAGCTT GAGTAGGCTC
    CATCCAGTCC CAGTCCCAGC AGATTGGATG AACCGCATGG AGGTGAATGA GACCCAGTAC
    AATGAGATGT TTGGTATGGT CCTGGCTCCA GCTGAGGGCA CCCATCCTGG CATAGCTCCC
    CAGAGCAGGC CACCTGAAGG GAAGCACATA ACCACCCCTC GGGAGCCCTT GCCCCCCATT
    GTGACTTTCC AGCTCATCCC TAAAGGCAGT ATTGCCCAAC CAGCGGGGGC TCCCCAGCCT
    CAGTCCACCT GCCCTCCAGC TGTTGCGGGC CCCCTGCCCA CCATGTACCA GATTCCAGAA
    ATGGCCCGTT TGCCCAGTGT GGCTTTCCCC ACTGCCATGA TGCCCCAGCA GGACGGGCAG
    GTAGCTCAGA CCATTCTCCC AGCCTATCAT CCTTTCCCAG CCTCTGTGGG CAAGTACCCC
    ACACCCCCTT CACAGCACAG TTATGCTTCC TCAAATGCTG CTGAGCGAAC ACCCAGTCAC
    AGTGGTCACC TCCAGGGTGA GCATCCCTAC CTGACACCAT CCCCAGAGTC TCCTGACCAG
    TGGTCAAGTT CATCACCCCA CTCTGCTTCT GACTGGTCAG ATGTGACCAC CAGCCCTACC
    CCTGGGGGTG CTGGAGGAGG TCAGCGGGGA CCTGGGACAC ACATGTCTGA GCCACCACAC
    AACAACATGC AGGTTTATGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011539821.1
    CDS5418..12821
    Translation

    Target ORF information:

    RefSeq Version XM_011541519.2
    Organism Homo sapiens(human)
    Definition Homo sapiens notch 2 (NOTCH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011541519.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGAACACAT ATTTGGTGTC GGCAGTTTCC CTCAGATGTG TTAGTGTGGA GAGATATAAA 
    GCATTGCAGT GTCGAGATGG CTATGAACCC TGTGTAAATG AAGGAATGTG TGTTACCTAC
    CACAATGGCA CAGGATACTG CAAATGTCCA GAAGGCTTCT TGGGGGAATA TTGTCAACAT
    CGAGACCCCT GTGAGAAGAA CCGCTGCCAG AATGGTGGGA CTTGTGTGGC CCAGGCCATG
    CTGGGGAAAG CCACGTGCCG ATGTGCCTCA GGGTTTACAG GAGAGGACTG CCAGTACTCA
    ACATCTCATC CATGCTTTGT GTCTCGACCC TGCCTGAATG GCGGCACATG CCATATGCTC
    AGCCGGGATA CCTATGAGTG CACCTGTCAA GTCGGGTTTA CAGGTAAGGA GTGCCAATGG
    ACGGATGCCT GCCTGTCTCA TCCCTGTGCA AATGGAAGTA CCTGTACCAC TGTGGCCAAC
    CAGTTCTCCT GCAAATGCCT CACAGGCTTC ACAGGGCAGA AATGTGAGAC TGATGTCAAT
    GAGTGTGACA TTCCAGGACA CTGCCAGCAT GGTGGCACCT GCCTCAACCT GCCTGGTTCC
    TACCAGTGCC AGTGCCCTCA GGGCTTCACA GGCCAGTACT GTGACAGCCT GTATGTGCCC
    TGTGCACCCT CACCTTGTGT CAATGGAGGC ACCTGTCGGC AGACTGGTGA CTTCACTTTT
    GAGTGCAACT GCCTTCCAGG TTTTGAAGGG AGCACCTGTG AGAGGAATAT TGATGACTGC
    CCTAACCACA GGTGTCAGAA TGGAGGGGTT TGTGTGGATG GGGTCAACAC TTACAACTGC
    CGCTGTCCCC CACAATGGAC AGGACAGTTC TGCACAGAGG ATGTGGATGA ATGCCTGCTG
    CAGCCCAATG CCTGTCAAAA TGGGGGCACC TGTGCCAACC GCAATGGAGG CTATGGCTGT
    GTATGTGTCA ACGGCTGGAG TGGAGATGAC TGCAGTGAGA ACATTGATGA TTGTGCCTTC
    GCCTCCTGTA CTCCAGGCTC CACCTGCATC GACCGTGTGG CCTCCTTCTC TTGCATGTGC
    CCAGAGGGGA AGGCAGGTCT CCTGTGTCAT CTGGATGATG CATGCATCAG CAATCCTTGC
    CACAAGGGGG CACTGTGTGA CACCAACCCC CTAAATGGGC AATATATTTG CACCTGCCCA
    CAAGGCTACA AAGGGGCTGA CTGCACAGAA GATGTGGATG AATGTGCCAT GGCCAATAGC
    AATCCTTGTG AGCATGCAGG AAAATGTGTG AACACGGATG GCGCCTTCCA CTGTGAGTGT
    CTGAAGGGTT ATGCAGGACC TCGTTGTGAG ATGGACATCA ATGAGTGCCA TTCAGACCCC
    TGCCAGAATG ATGCTACCTG TCTGGATAAG ATTGGAGGCT TCACATGTCT GTGCATGCCA
    GGTTTCAAAG GTGTGCATTG TGAATTAGAA ATAAATGAAT GTCAGAGCAA CCCTTGTGTG
    AACAATGGGC AGTGTGTGGA TAAAGTCAAT CGTTTCCAGT GCCTGTGTCC TCCTGGTTTC
    ACTGGGCCAG TTTGCCAGAT TGATATTGAT GACTGTTCCA GTACTCCGTG TCTGAATGGG
    GCAAAGTGTA TCGATCACCC GAATGGCTAT GAATGCCAGT GTGCCACAGG TTTCACTGGT
    GTGTTGTGTG AGGAGAACAT TGACAACTGT GACCCCGATC CTTGCCACCA TGGTCAGTGT
    CAGGATGGTA TTGATTCCTA CACCTGCATC TGCAATCCCG GGTACATGGG CGCCATCTGC
    AGTGACCAGA TTGATGAATG TTACAGCAGC CCTTGCCTGA ACGATGGTCG CTGCATTGAC
    CTGGTCAATG GCTACCAGTG CAACTGCCAG CCAGGCACGT CAGGGGTTAA TTGTGAAATT
    AATTTTGATG ACTGTGCAAG TAACCCTTGT ATCCATGGAA TCTGTATGGA TGGCATTAAT
    CGCTACAGTT GTGTCTGCTC ACCAGGATTC ACAGGGCAGA GATGTAACAT TGACATTGAT
    GAGTGTGCCT CCAATCCCTG TCGCAAGGGT GCAACATGTA TCAACGGTGT GAATGGTTTC
    CGCTGTATAT GCCCCGAGGG ACCCCATCAC CCCAGCTGCT ACTCACAGGT GAACGAATGC
    CTGAGCAATC CCTGCATCCA TGGAAACTGT ACTGGAGGTC TCAGTGGATA TAAGTGTCTC
    TGTGATGCAG GCTGGGTTGG CATCAACTGT GAAGTGGACA AAAATGAATG CCTTTCGAAT
    CCATGCCAGA ATGGAGGAAC TTGTGACAAT CTGGTGAATG GATACAGGTG TACTTGCAAG
    AAGGGCTTTA AAGGCTATAA CTGCCAGGTG AATATTGATG AATGTGCCTC AAATCCATGC
    CTGAACCAAG GAACCTGCTT TGATGACATA AGTGGCTACA CTTGCCACTG TGTGCTGCCA
    TACACAGGCA AGAATTGTCA GACAGTATTG GCTCCCTGTT CCCCAAACCC TTGTGAGAAT
    GCTGCTGTTT GCAAAGAGTC ACCAAATTTT GAGAGTTATA CTTGCTTGTG TGCTCCTGGC
    TGGCAAGGTC AGCGGTGTAC CATTGACATT GACGAGTGTA TCTCCAAGCC CTGCATGAAC
    CATGGTCTCT GCCATAACAC CCAGGGCAGC TACATGTGTG AATGTCCACC AGGCTTCAGT
    GGTATGGACT GTGAGGAGGA CATTGATGAC TGCCTTGCCA ATCCTTGCCA GAATGGAGGT
    TCCTGTATGG ATGGAGTGAA TACTTTCTCC TGCCTCTGCC TTCCGGGTTT CACTGGGGAT
    AAGTGCCAGA CAGACATGAA TGAGTGTCTG AGTGAACCCT GTAAGAATGG AGGGACCTGC
    TCTGACTACG TCAACAGTTA CACTTGCAAG TGCCAGGCAG GATTTGATGG AGTCCATTGT
    GAGAACAACA TCAATGAGTG CACTGAGAGC TCCTGTTTCA ATGGTGGCAC ATGTGTTGAT
    GGGATTAACT CCTTCTCTTG CTTGTGCCCT GTGGGTTTCA CTGGATCCTT CTGCCTCCAT
    GAGATCAATG AATGCAGCTC TCATCCATGC CTGAATGAGG GAACGTGTGT TGATGGCCTG
    GGTACCTACC GCTGCAGCTG CCCCCTGGGC TACACTGGGA AAAACTGTCA GACCCTGGTG
    AATCTCTGCA GTCGGTCTCC ATGTAAAAAC AAAGGTACTT GCGTTCAGAA AAAAGCAGAG
    TCCCAGTGCC TATGTCCATC TGGATGGGCT GGTGCCTATT GTGACGTGCC CAATGTCTCT
    TGTGACATAG CAGCCTCCAG GAGAGGTGTG CTTGTTGAAC ACTTGTGCCA GCACTCAGGT
    GTCTGCATCA ATGCTGGCAA CACGCATTAC TGTCAGTGCC CCCTGGGCTA TACTGGGAGC
    TACTGTGAGG AGCAACTCGA TGAGTGTGCG TCCAACCCCT GCCAGCACGG GGCAACATGC
    AGTGACTTCA TTGGTGGATA CAGATGCGAG TGTGTCCCAG GCTATCAGGG TGTCAACTGT
    GAGTATGAAG TGGATGAGTG CCAGAATCAG CCCTGCCAGA ATGGAGGCAC CTGTATTGAC
    CTTGTGAACC ATTTCAAGTG CTCTTGCCCA CCAGGCACTC GGGGCCTACT CTGTGAAGAG
    AACATTGATG ACTGTGCCCG GGGTCCCCAT TGCCTTAATG GTGGTCAGTG CATGGATAGG
    ATTGGAGGCT ACAGTTGTCG CTGCTTGCCT GGCTTTGCTG GGGAGCGTTG TGAGGGAGAC
    ATCAACGAGT GCCTCTCCAA CCCCTGCAGC TCTGAGGGCA GCCTGGACTG TATACAGCTC
    ACCAATGACT ACCTGTGTGT TTGCCGTAGT GCCTTTACTG GCCGGCACTG TGAAACCTTC
    GTCGATGTGT GTCCCCAGAT GCCCTGCCTG AATGGAGGGA CTTGTGCTGT GGCCAGTAAC
    ATGCCTGATG GTTTCATTTG CCGTTGTCCC CCGGGATTTT CCGGGGCAAG GTGCCAGAGC
    AGCTGTGGAC AAGTGAAATG TAGGAAGGGG GAGCAGTGTG TGCACACCGC CTCTGGACCC
    CGCTGCTTCT GCCCCAGTCC CCGGGACTGC GAGTCAGGCT GTGCCAGTAG CCCCTGCCAG
    CACGGGGGCA GCTGCCACCC TCAGCGCCAG CCTCCTTATT ACTCCTGCCA GTGTGCCCCA
    CCATTCTCGG GTAGCCGCTG TGAACTCTAC ACGGCACCCC CCAGCACCCC TCCTGCCACC
    TGTCTGAGCC AGTATTGTGC CGACAAAGCT CGGGATGGCG TCTGTGATGA GGCCTGCAAC
    AGCCATGCCT GCCAGTGGGA TGGGGGTGAC TGTTCTCTCA CCATGGAGAA CCCCTGGGCC
    AACTGCTCCT CCCCACTTCC CTGCTGGGAT TATATCAACA ACCAGTGTGA TGAGCTGTGC
    AACACGGTCG AGTGCCTGTT TGACAACTTT GAATGCCAGG GGAACAGCAA GACATGCAAG
    TATGACAAAT ACTGTGCAGA CCACTTCAAA GACAACCACT GTGACCAGGG GTGCAACAGT
    GAGGAGTGTG GTTGGGATGG GCTGGACTGT GCTGCTGACC AACCTGAGAA CCTGGCAGAA
    GGTACCCTGG TTATTGTGGT ATTGATGCCA CCTGAACAAC TGCTCCAGGA TGCTCGCAGC
    TTCTTGCGGG CACTGGGTAC CCTGCTCCAC ACCAACCTGC GCATTAAGCG GGACTCCCAG
    GGGGAACTCA TGGTGTACCC CTATTATGGT GAGAAGTCAG CTGCTATGAA GAAACAGAGG
    ATGACACGCA GATCCCTTCC TGGTGAACAA GAACAGGAGG TGGCTGGCTC TAAAGTCTTT
    CTGGAAATTG ACAACCGCCA GTGTGTTCAA GACTCAGACC ACTGCTTCAA GAACACGGAT
    GCAGCAGCAG CTCTCCTGGC CTCTCACGCC ATACAGGGGA CCCTGTCATA CCCTCTTGTG
    TCTGTCGTCA GTGAATCCCT GACTCCAGAA CGCACTCAGC TCCTCTATCT CCTTGCTGTT
    GCTGTTGTCA TCATTCTGTT TATTATTCTG CTGGGGGTAA TCATGGCAAA ACGAAAGCGT
    AAGCATGGCT CTCTCTGGCT GCCTGAAGGT TTCACTCTTC GCCGAGATGC AAGCAATCAC
    AAGCGTCGTG AGCCAGTGGG ACAGGATGCT GTGGGGCTGA AAAATCTCTC AGTGCAAGTC
    TCAGAAGCTA ACCTAATTGG TACTGGAACA AGTGAACACT GGGTCGATGA TGAAGGGCCC
    CAGCCAAAGA AAGTAAAGGC TGAAGATGAG GCCTTACTCT CAGAAGAAGA TGACCCCATT
    GATCGACGGC CATGGACACA GCAGCACCTT GAAGCTGCAG ACATCCGTAG GACACCATCG
    CTGGCTCTCA CCCCTCCTCA GGCAGAGCAG GAGGTGGATG TGTTAGATGT GAATGTCCGT
    GGCCCAGATG GCTGCACCCC ATTGATGTTG GCTTCTCTCC GAGGAGGCAG CTCAGATTTG
    AGTGATGAAG ATGAAGATGC AGAGGACTCT TCTGCTAACA TCATCACAGA CTTGGTCTAC
    CAGGGTGCCA GCCTCCAGGC CCAGACAGAC CGGACTGGTG AGATGGCCCT GCACCTTGCA
    GCCCGCTACT CACGGGCTGA TGCTGCCAAG CGTCTCCTGG ATGCAGGTGC AGATGCCAAT
    GCCCAGGACA ACATGGGCCG CTGTCCACTC CATGCTGCAG TGGCAGCTGA TGCCCAAGGT
    GTCTTCCAGA TTCTGATTCG CAACCGAGTA ACTGATCTAG ATGCCAGGAT GAATGATGGT
    ACTACACCCC TGATCCTGGC TGCCCGCCTG GCTGTGGAGG GAATGGTGGC AGAACTGATC
    AACTGCCAAG CGGATGTGAA TGCAGTGGAT GACCATGGAA AATCTGCTCT TCACTGGGCA
    GCTGCTGTCA ATAATGTGGA GGCAACTCTT TTGTTGTTGA AAAATGGGGC CAACCGAGAC
    ATGCAGGACA ACAAGGAAGA GACACCTCTG TTTCTTGCTG CCCGGGAGGG GAGCTATGAA
    GCAGCCAAGA TCCTGTTAGA CCATTTTGCC AATCGAGACA TCACAGACCA TATGGATCGT
    CTTCCCCGGG ATGTGGCTCG GGATCGCATG CACCATGACA TTGTGCGCCT TCTGGATGAA
    TACAATGTGA CCCCAAGCCC TCCAGGCACC GTGTTGACTT CTGCTCTCTC ACCTGTCATC
    TGTGGGCCCA ACAGATCTTT CCTCAGCCTG AAGCACACCC CAATGGGCAA GAAGTCTAGA
    CGGCCCAGTG CCAAGAGTAC CATGCCTACT AGCCTCCCTA ACCTTGCCAA GGAGGCAAAG
    GATGCCAAGG GTAGTAGGAG GAAGAAGTCT CTGAGTGAGA AGGTCCAACT GTCTGAGAGT
    TCAGTAACTT TATCCCCTGT TGATTCCCTA GAATCTCCTC ACACGTATGT TTCCGACACC
    ACATCCTCTC CAATGATTAC ATCCCCTGGG ATCTTACAGG CCTCACCCAA CCCTATGTTG
    GCCACTGCCG CCCCTCCTGC CCCAGTCCAT GCCCAGCATG CACTATCTTT TTCTAACCTT
    CATGAAATGC AGCCTTTGGC ACATGGGGCC AGCACTGTGC TTCCCTCAGT GAGCCAGTTG
    CTATCCCACC ACCACATTGT GTCTCCAGGC AGTGGCAGTG CTGGAAGCTT GAGTAGGCTC
    CATCCAGTCC CAGTCCCAGC AGATTGGATG AACCGCATGG AGGTGAATGA GACCCAGTAC
    AATGAGATGT TTGGTATGGT CCTGGCTCCA GCTGAGGGCA CCCATCCTGG CATAGCTCCC
    CAGAGCAGGC CACCTGAAGG GAAGCACATA ACCACCCCTC GGGAGCCCTT GCCCCCCATT
    GTGACTTTCC AGCTCATCCC TAAAGGCAGT ATTGCCCAAC CAGCGGGGGC TCCCCAGCCT
    CAGTCCACCT GCCCTCCAGC TGTTGCGGGC CCCCTGCCCA CCATGTACCA GATTCCAGAA
    ATGGCCCGTT TGCCCAGTGT GGCTTTCCCC ACTGCCATGA TGCCCCAGCA GGACGGGCAG
    GTAGCTCAGA CCATTCTCCC AGCCTATCAT CCTTTCCCAG CCTCTGTGGG CAAGTACCCC
    ACACCCCCTT CACAGCACAG TTATGCTTCC TCAAATGCTG CTGAGCGAAC ACCCAGTCAC
    AGTGGTCACC TCCAGGGTGA GCATCCCTAC CTGACACCAT CCCCAGAGTC TCCTGACCAG
    TGGTCAAGTT CATCACCCCA CTCTGCTTCT GACTGGTCAG ATGTGACCAC CAGCCCTACC
    CCTGGGGGTG CTGGAGGAGG TCAGCGGGGA CCTGGGACAC ACATGTCTGA GCCACCACAC
    AACAACATGC AGGTTTATGC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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