• English
  • Sign In
    Please Sign In
    Sign In
    New to GenScript?
    Register
  • Contact Us
×
Contact us now: +1-877-436-7274

NOTCH3 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol NOTCH3
Entrez Gene ID 4854
Full Name notch 3
Synonyms CADASIL,CADASIL1,CASIL,IMF2,LMNS
General protein information
Preferred Names

notch 3

Names

neurogenic locus notch homolog protein 3
Notch homolog 3
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

19

19p13.12
Summary This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). [provided by RefSeq, Jul 2008].
Disorder MIM:

600276

Disorder Html: Cerebral arteriopathy with subcortical infarcts and leukoencephalopathy 1, 125310 (3); ?Myofibromatosis, infantile 2, 615293 (3); Lateral meningocele syndrome, 130720 (3)

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_000435.3 NP_000426.2 neurogenic locus notch homolog protein 3 precursor
NM_000435.2 NP_000426.2 neurogenic locus notch homolog protein 3 precursor
XM_005259924.4 XP_005259981.1 neurogenic locus notch homolog protein 3 isoform X1

Pathways from BioSystems


Homologies


Pan troglodytes (chimpanzee) NOTCH3 XP_003316212.1
NOTCH3 XP_002808222.1
Bos taurus (cattle) NOTCH3 XP_003586294.1
Canis lupus familiaris (dog) NOTCH3 XP_005633258.1
Mus musculus (house mouse) Notch3 NP_032742.1
Xenopus tropicalis (tropical clawed frog) notch2 XP_002941531.2
Homo sapiens (human) NOTCH3 NP_000426.2
Rattus norvegicus (Norway rat) Notch3 NP_064472.2

Gene Ontology


Bibliography

Related articles in PubMed

The common oncogenomic program of NOTCH1 and NOTCH3 signaling in T-cell acute lymphoblastic leukemia.
Choi SH, Severson E, Pear WS, Liu XS, Aster JC, Blacklow SC
PloS one12(10)e0185762(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Single-nucleotide polymorphism in NOTCH3 gene is associated with breast cancers.
Title: Identification of Novel Breast Cancer Risk Loci.

Oligodendrocytes expressing mutant NOTCH3(R90C) (present in CADASIL disease), exhibited aberrant NOTCH3 proteolytic processing. These cells were less viable and had a higher rate of apoptosis. Cells with NOTCH3(R90C) had higher levels of intrinsic mitochondrial apoptosis, extrinsic death receptor path-related apoptosis, and autophagy compared with cells transfected with wild-type NOTCH3.
Title: CADASIL mutant NOTCH3(R90C) decreases the viability of HS683 oligodendrocytes via apoptosis.

Specific Notch3 and Jag1 subcellular localization patterns may provide clues for the behavior of the corresponding tumors and could potentially be applied in the clinic for Jag1 targeting in triple-negative breast cancer patients
Title: Prognostic Subcellular Notch2, Notch3 and Jagged1 Localization Patterns in Early Triple-negative Breast Cancer.

there is a cross-talk between Jagged1/Notch3 and VEGF in TNBC angiogenesis. Jagged1/Notch3 is expected to be an important signaling pathway for TNBC progression and a potential target for TNBC neovascularization therapy.
Title: Expression of Jagged1/Notch3 Signaling Pathway and their Relationship with the Tumor Angiogenesis in TNBC.

The mRNA and protein expression of NOTCH3 was significantly lower in cisplatin and astragaloside IV-treated cells.
Title: Astragaloside IV Enhances Cisplatin Chemosensitivity in Human Colorectal Cancer via Regulating NOTCH3.

The following NOTCH3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOTCH3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu24165 NM_000435.3
Latest version!		 Homo sapiens notch receptor 3 (NOTCH3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $659.50-$923.30
$1319.00
OHu51627 XM_005259924.4
Latest version!		 Homo sapiens notch receptor 3 (NOTCH3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector TBD $1091.30
$1559.00
View Old Accession Versions >>
OHu24165 NM_000435.2 Homo sapiens notch 3 (NOTCH3), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $659.50-$923.30
$1319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu24165
Clone ID Related Accession (Same CDS sequence) NM_000435.3 , NM_000435.2
Accession Version NM_000435.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 6966bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-30
Organism Homo sapiens(human)
Product neurogenic locus notch homolog protein 3 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004257.1, U97669.1 and AC004663.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_000435.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U97669.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263388.7/ ENSP00000263388.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
ATGGGGCCGG GGGCCCGTGG CCGCCGCCGC CGCCGTCGCC CGATGTCGCC GCCACCGCCA 
CCGCCACCCG TGCGGGCGCT GCCCCTGCTG CTGCTGCTAG CGGGGCCGGG GGCTGCAGCC 
CCCCCTTGCC TGGACGGAAG CCCGTGTGCA AATGGAGGTC GTTGCACCCA GCTGCCCTCC 
CGGGAGGCTG CCTGCCTGTG CCCGCCTGGC TGGGTGGGTG AGCGGTGTCA GCTGGAGGAC 
CCCTGTCACT CAGGCCCCTG TGCTGGCCGT GGTGTCTGCC AGAGTTCAGT GGTGGCTGGC 
ACCGCCCGAT TCTCATGCCG GTGCCCCCGT GGCTTCCGAG GCCCTGACTG CTCCCTGCCA 
GATCCCTGCC TCAGCAGCCC TTGTGCCCAC GGTGCCCGCT GCTCAGTGGG GCCCGATGGA 
CGCTTCCTCT GCTCCTGCCC ACCTGGCTAC CAGGGCCGCA GCTGCCGAAG CGACGTGGAT 
GAGTGCCGGG TGGGTGAGCC CTGCCGCCAT GGTGGCACCT GCCTCAACAC ACCTGGCTCC 
TTCCGCTGCC AGTGTCCAGC TGGCTACACA GGGCCACTAT GTGAGAACCC CGCGGTGCCC 
TGTGCACCCT CACCATGCCG TAACGGGGGC ACCTGCAGGC AGAGTGGCGA CCTCACTTAC 
GACTGTGCCT GTCTTCCTGG GTTTGAGGGT CAGAATTGTG AAGTGAACGT GGACGACTGT 
CCAGGACACC GATGTCTCAA TGGGGGGACA TGCGTGGATG GCGTCAACAC CTATAACTGC 
CAGTGCCCTC CTGAGTGGAC AGGCCAGTTC TGCACGGAGG ACGTGGATGA GTGTCAGCTG 
CAGCCCAACG CCTGCCACAA TGGGGGTACC TGCTTCAACA CGCTGGGTGG CCACAGCTGC 
GTGTGTGTCA ATGGCTGGAC AGGCGAGAGC TGCAGTCAGA ATATCGATGA CTGTGCCACA 
GCCGTGTGCT TCCATGGGGC CACCTGCCAT GACCGCGTGG CTTCTTTCTA CTGTGCCTGC 
CCCATGGGCA AGACTGGCCT CCTGTGTCAC CTGGATGACG CCTGTGTCAG CAACCCCTGC 
CACGAGGATG CTATCTGTGA CACAAATCCG GTGAACGGCC GGGCCATTTG CACCTGTCCT 
CCCGGCTTCA CGGGTGGGGC ATGTGACCAG GATGTGGACG AGTGCTCTAT CGGCGCCAAC 
CCCTGCGAGC ACTTGGGCAG GTGCGTGAAC ACGCAGGGCT CCTTCCTGTG CCAGTGCGGT 
CGTGGCTACA CTGGACCTCG CTGTGAGACC GATGTCAACG AGTGTCTGTC GGGGCCCTGC 
CGAAACCAGG CCACGTGCCT CGACCGCATA GGCCAGTTCA CCTGTATCTG TATGGCAGGC 
TTCACAGGAA CCTATTGCGA GGTGGACATT GACGAGTGTC AGAGTAGCCC CTGTGTCAAC 
GGTGGGGTCT GCAAGGACCG AGTCAATGGC TTCAGCTGCA CCTGCCCCTC GGGCTTCAGC 
GGCTCCACGT GTCAGCTGGA CGTGGACGAA TGCGCCAGCA CGCCCTGCAG GAATGGCGCC 
AAATGCGTGG ACCAGCCCGA TGGCTACGAG TGCCGCTGTG CCGAGGGCTT TGAGGGCACG 
CTGTGTGATC GCAACGTGGA CGACTGCTCC CCTGACCCAT GCCACCATGG TCGCTGCGTG 
GATGGCATCG CCAGCTTCTC ATGTGCCTGT GCTCCTGGCT ACACGGGCAC ACGCTGCGAG 
AGCCAGGTGG ACGAATGCCG CAGCCAGCCC TGCCGCCATG GCGGCAAATG CCTAGACCTG 
GTGGACAAGT ACCTCTGCCG CTGCCCTTCT GGGACCACAG GTGTGAACTG CGAAGTGAAC 
ATTGACGACT GTGCCAGCAA CCCCTGCACC TTTGGAGTCT GCCGTGATGG CATCAACCGC 
TACGACTGTG TCTGCCAACC TGGCTTCACA GGGCCCCTTT GTAACGTGGA GATCAATGAG 
TGTGCTTCCA GCCCATGCGG CGAGGGAGGT TCCTGTGTGG ATGGGGAAAA TGGCTTCCGC 
TGCCTCTGCC CGCCTGGCTC CTTGCCCCCA CTCTGCCTCC CCCCGAGCCA TCCCTGTGCC 
CATGAGCCCT GCAGTCACGG CATCTGCTAT GATGCACCTG GCGGGTTCCG CTGTGTGTGT 
GAGCCTGGCT GGAGTGGCCC CCGCTGCAGC CAGAGCCTGG CCCGAGACGC CTGTGAGTCC 
CAGCCGTGCA GGGCCGGTGG GACATGCAGC AGCGATGGAA TGGGTTTCCA CTGCACCTGC 
CCGCCTGGTG TCCAGGGACG TCAGTGTGAA CTCCTCTCCC CCTGCACCCC GAACCCCTGT 
GAGCATGGGG GCCGCTGCGA GTCTGCCCCT GGCCAGCTGC CTGTCTGCTC CTGCCCCCAG 
GGCTGGCAAG GCCCACGATG CCAGCAGGAT GTGGACGAGT GTGCTGGCCC CGCACCCTGT 
GGCCCTCATG GTATCTGCAC CAACCTGGCA GGGAGTTTCA GCTGCACCTG CCATGGAGGG 
TACACTGGCC CTTCCTGCGA TCAGGACATC AATGACTGTG ACCCCAACCC ATGCCTGAAC 
GGTGGCTCGT GCCAAGACGG CGTGGGCTCC TTTTCCTGCT CCTGCCTCCC TGGTTTCGCC 
GGCCCACGAT GCGCCCGCGA TGTGGATGAG TGCCTGAGCA ACCCCTGCGG CCCGGGCACC 
TGTACCGACC ACGTGGCCTC CTTCACCTGC ACCTGCCCGC CAGGCTACGG AGGCTTCCAC 
TGCGAACAGG ACCTGCCCGA CTGCAGCCCC AGCTCCTGCT TCAATGGCGG GACCTGTGTG 
GACGGCGTGA ACTCGTTCAG CTGCCTGTGC CGTCCCGGCT ACACAGGAGC CCACTGCCAA 
CATGAGGCAG ACCCCTGCCT CTCGCGGCCC TGCCTACACG GGGGCGTCTG CAGCGCCGCC 
CACCCTGGCT TCCGCTGCAC CTGCCTCGAG AGCTTCACGG GCCCGCAGTG CCAGACGCTG 
GTGGATTGGT GCAGCCGCCA GCCTTGTCAA AACGGGGGTC GCTGCGTCCA GACTGGGGCC 
TATTGCCTTT GTCCCCCTGG ATGGAGCGGA CGCCTCTGTG ACATCCGAAG CTTGCCCTGC 
AGGGAGGCCG CAGCCCAGAT CGGGGTGCGG CTGGAGCAGC TGTGTCAGGC GGGTGGGCAG 
TGTGTGGATG AAGACAGCTC CCACTACTGC GTGTGCCCAG AGGGCCGTAC TGGTAGCCAC 
TGTGAGCAGG AGGTGGACCC CTGCTTGGCC CAGCCCTGCC AGCATGGGGG GACCTGCCGT 
GGCTATATGG GGGGCTACAT GTGTGAGTGT CTTCCTGGCT ACAATGGTGA TAACTGTGAG 
GACGACGTGG ACGAGTGTGC CTCCCAGCCC TGCCAGCACG GGGGTTCATG CATTGACCTC 
GTGGCCCGCT ATCTCTGCTC CTGTCCCCCA GGAACGCTGG GGGTGCTCTG CGAGATTAAT 
GAGGATGACT GCGGCCCAGG CCCACCGCTG GACTCAGGGC CCCGGTGCCT ACACAATGGC 
ACCTGCGTGG ACCTGGTGGG TGGTTTCCGC TGCACCTGTC CCCCAGGATA CACTGGTTTG 
CGCTGCGAGG CAGACATCAA TGAGTGTCGC TCAGGTGCCT GCCACGCGGC ACACACCCGG 
GACTGCCTGC AGGACCCAGG CGGAGGTTTC CGTTGCCTTT GTCATGCTGG CTTCTCAGGT 
CCTCGCTGTC AGACTGTCCT GTCTCCCTGC GAGTCCCAGC CATGCCAGCA TGGAGGCCAG 
TGCCGTCCTA GCCCGGGTCC TGGGGGTGGG CTGACCTTCA CCTGTCACTG TGCCCAGCCG 
TTCTGGGGTC CGCGTTGCGA GCGGGTGGCG CGCTCCTGCC GGGAGCTGCA GTGCCCGGTG 
GGCGTCCCAT GCCAGCAGAC GCCCCGCGGG CCGCGCTGCG CCTGCCCCCC AGGGTTGTCG 
GGACCCTCCT GCCGCAGCTT CCCGGGGTCG CCGCCGGGGG CCAGCAACGC CAGCTGCGCG 
GCCGCCCCCT GTCTCCACGG GGGCTCCTGC CGCCCCGCGC CGCTCGCGCC CTTCTTCCGC 
TGCGCTTGCG CGCAGGGCTG GACCGGGCCG CGCTGCGAGG CGCCCGCCGC GGCACCCGAG 
GTCTCGGAGG AGCCGCGGTG CCCGCGCGCC GCCTGCCAGG CCAAGCGCGG GGACCAGCGC 
TGCGACCGCG AGTGCAACAG CCCAGGCTGC GGCTGGGACG GCGGCGACTG CTCGCTGAGC 
GTGGGCGACC CCTGGCGGCA ATGCGAGGCG CTGCAGTGCT GGCGCCTCTT CAACAACAGC 
CGCTGCGACC CCGCCTGCAG CTCGCCCGCC TGCCTCTACG ACAACTTCGA CTGCCACGCC 
GGTGGCCGCG AGCGCACTTG CAACCCGGTG TACGAGAAGT ACTGCGCCGA CCACTTTGCC 
GACGGCCGCT GCGACCAGGG CTGCAACACG GAGGAGTGCG GCTGGGATGG GCTGGATTGT 
GCCAGCGAGG TGCCGGCCCT GCTGGCCCGC GGCGTGCTGG TGCTCACAGT GCTGCTGCCG 
CCAGAGGAGC TACTGCGTTC CAGCGCCGAC TTTCTGCAGC GGCTCAGCGC CATCCTGCGC 
ACCTCGCTGC GCTTCCGCCT GGACGCGCAC GGCCAGGCCA TGGTCTTCCC TTACCACCGG 
CCTAGTCCTG GCTCCGAACC CCGGGCCCGT CGGGAGCTGG CCCCCGAGGT GATCGGCTCG 
GTAGTAATGC TGGAGATTGA CAACCGGCTC TGCCTGCAGT CGCCTGAGAA TGATCACTGC 
TTCCCCGATG CCCAGAGCGC CGCTGACTAC CTGGGAGCGT TGTCAGCGGT GGAGCGCCTG 
GACTTCCCGT ACCCACTGCG GGACGTGCGG GGGGAGCCGC TGGAGCCTCC AGAACCCAGC 
GTCCCGCTGC TGCCACTGCT AGTGGCGGGC GCTGTCTTGC TGCTGGTCAT TCTCGTCCTG 
GGTGTCATGG TGGCCCGGCG CAAGCGCGAG CACAGCACCC TCTGGTTCCC TGAGGGCTTC 
TCACTGCACA AGGACGTGGC CTCTGGTCAC AAGGGCCGGC GGGAACCCGT GGGCCAGGAC 
GCGCTGGGCA TGAAGAACAT GGCCAAGGGT GAGAGCCTGA TGGGGGAGGT GGCCACAGAC 
TGGATGGACA CAGAGTGCCC AGAGGCCAAG CGGCTAAAGG TAGAGGAGCC AGGCATGGGG 
GCTGAGGAGG CTGTGGATTG CCGTCAGTGG ACTCAACACC ATCTGGTTGC TGCTGACATC 
CGCGTGGCAC CAGCCATGGC ACTGACACCA CCACAGGGCG ACGCAGATGC TGATGGCATG 
GATGTCAATG TGCGTGGCCC AGATGGCTTC ACCCCGCTAA TGCTGGCTTC CTTCTGTGGG 
GGGGCTCTGG AGCCAATGCC AACTGAAGAG GATGAGGCAG ATGACACATC AGCTAGCATC 
ATCTCCGACC TGATCTGCCA GGGGGCTCAG CTTGGGGCAC GGACTGACCG TACTGGCGAG 
ACTGCTTTGC ACCTGGCTGC CCGTTATGCC CGTGCTGATG CAGCCAAGCG GCTGCTGGAT 
GCTGGGGCAG ACACCAATGC CCAGGACCAC TCAGGCCGCA CTCCCCTGCA CACAGCTGTC 
ACAGCCGATG CCCAGGGTGT CTTCCAGATT CTCATCCGAA ACCGCTCTAC AGACTTGGAT 
GCCCGCATGG CAGATGGCTC AACGGCACTG ATCCTGGCGG CCCGCCTGGC AGTAGAGGGC 
ATGGTGGAAG AGCTCATCGC CAGCCATGCT GATGTCAATG CTGTGGATGA GCTTGGGAAA 
TCAGCCTTAC ACTGGGCTGC GGCTGTGAAC AACGTGGAAG CCACTTTGGC CCTGCTCAAA 
AATGGAGCCA ATAAGGACAT GCAGGATAGC AAGGAGGAGA CCCCCCTATT CCTGGCCGCC 
CGCGAGGGCA GCTATGAGGC TGCCAAGCTG CTGTTGGACC ACTTTGCCAA CCGTGAGATC 
ACCGACCACC TGGACAGGCT GCCGCGGGAC GTAGCCCAGG AGAGACTGCA CCAGGACATC 
GTGCGCTTGC TGGATCAACC CAGTGGGCCC CGCAGCCCCC CCGGTCCCCA CGGCCTGGGG 
CCTCTGCTCT GTCCTCCAGG GGCCTTCCTC CCTGGCCTCA AAGCGGCACA GTCGGGGTCC 
AAGAAGAGCA GGAGGCCCCC CGGGAAGGCG GGGCTGGGGC CGCAGGGGCC CCGGGGGCGG 
GGCAAGAAGC TGACGCTGGC CTGCCCGGGC CCCCTGGCTG ACAGCTCGGT CACGCTGTCG 
CCCGTGGACT CGCTGGACTC CCCGCGGCCT TTCGGTGGGC CCCCTGCTTC CCCTGGTGGC 
TTCCCCCTTG AGGGGCCCTA TGCAGCTGCC ACTGCCACTG CAGTGTCTCT GGCACAGCTT 
GGTGGCCCAG GCCGGGCGGG TCTAGGGCGC CAGCCCCCTG GAGGATGTGT ACTCAGCCTG 
GGCCTGCTGA ACCCTGTGGC TGTGCCCCTC GATTGGGCCC GGCTGCCCCC ACCTGCCCCT 
CCAGGCCCCT CGTTCCTGCT GCCACTGGCG CCGGGACCCC AGCTGCTCAA CCCAGGGACC 
CCCGTCTCCC CGCAGGAGCG GCCCCCGCCT TACCTGGCAG TCCCAGGACA TGGCGAGGAG 
TACCCGGCGG CTGGGGCACA CAGCAGCCCC CCAAAGGCCC GCTTCCTGCG GGTTCCCAGT 
GAGCACCCTT ACCTGACCCC ATCCCCCGAA TCCCCTGAGC ACTGGGCCAG CCCCTCACCT 
CCCTCCCTCT CAGACTGGTC CGAATCCACG CCTAGCCCAG CCACTGCCAC TGGGGCCATG 
GCCACCACCA CTGGGGCACT GCCTGCCCAG CCACTTCCCT TGTCTGTTCC CAGCTCCCTT 
GCTCAGGCCC AGACCCAGCT GGGGCCCCAG CCGGAAGTTA CCCCCAAGAG GCAAGTGTTG 
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000426.2
CDS91..7056
Translation

Target ORF information:

RefSeq Version NM_000435.3
Organism Homo sapiens(human)
Definition Homo sapiens notch receptor 3 (NOTCH3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_000435.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
ATGGGGCCGG GGGCCCGTGG CCGCCGCCGC CGCCGTCGCC CGATGTCGCC GCCACCGCCA 
CCGCCACCCG TGCGGGCGCT GCCCCTGCTG CTGCTGCTAG CGGGGCCGGG GGCTGCAGCC 
CCCCCTTGCC TGGACGGAAG CCCGTGTGCA AATGGAGGTC GTTGCACCCA GCTGCCCTCC 
CGGGAGGCTG CCTGCCTGTG CCCGCCTGGC TGGGTGGGTG AGCGGTGTCA GCTGGAGGAC 
CCCTGTCACT CAGGCCCCTG TGCTGGCCGT GGTGTCTGCC AGAGTTCAGT GGTGGCTGGC 
ACCGCCCGAT TCTCATGCCG GTGCCCCCGT GGCTTCCGAG GCCCTGACTG CTCCCTGCCA 
GATCCCTGCC TCAGCAGCCC TTGTGCCCAC GGTGCCCGCT GCTCAGTGGG GCCCGATGGA 
CGCTTCCTCT GCTCCTGCCC ACCTGGCTAC CAGGGCCGCA GCTGCCGAAG CGACGTGGAT 
GAGTGCCGGG TGGGTGAGCC CTGCCGCCAT GGTGGCACCT GCCTCAACAC ACCTGGCTCC 
TTCCGCTGCC AGTGTCCAGC TGGCTACACA GGGCCACTAT GTGAGAACCC CGCGGTGCCC 
TGTGCACCCT CACCATGCCG TAACGGGGGC ACCTGCAGGC AGAGTGGCGA CCTCACTTAC 
GACTGTGCCT GTCTTCCTGG GTTTGAGGGT CAGAATTGTG AAGTGAACGT GGACGACTGT 
CCAGGACACC GATGTCTCAA TGGGGGGACA TGCGTGGATG GCGTCAACAC CTATAACTGC 
CAGTGCCCTC CTGAGTGGAC AGGCCAGTTC TGCACGGAGG ACGTGGATGA GTGTCAGCTG 
CAGCCCAACG CCTGCCACAA TGGGGGTACC TGCTTCAACA CGCTGGGTGG CCACAGCTGC 
GTGTGTGTCA ATGGCTGGAC AGGCGAGAGC TGCAGTCAGA ATATCGATGA CTGTGCCACA 
GCCGTGTGCT TCCATGGGGC CACCTGCCAT GACCGCGTGG CTTCTTTCTA CTGTGCCTGC 
CCCATGGGCA AGACTGGCCT CCTGTGTCAC CTGGATGACG CCTGTGTCAG CAACCCCTGC 
CACGAGGATG CTATCTGTGA CACAAATCCG GTGAACGGCC GGGCCATTTG CACCTGTCCT 
CCCGGCTTCA CGGGTGGGGC ATGTGACCAG GATGTGGACG AGTGCTCTAT CGGCGCCAAC 
CCCTGCGAGC ACTTGGGCAG GTGCGTGAAC ACGCAGGGCT CCTTCCTGTG CCAGTGCGGT 
CGTGGCTACA CTGGACCTCG CTGTGAGACC GATGTCAACG AGTGTCTGTC GGGGCCCTGC 
CGAAACCAGG CCACGTGCCT CGACCGCATA GGCCAGTTCA CCTGTATCTG TATGGCAGGC 
TTCACAGGAA CCTATTGCGA GGTGGACATT GACGAGTGTC AGAGTAGCCC CTGTGTCAAC 
GGTGGGGTCT GCAAGGACCG AGTCAATGGC TTCAGCTGCA CCTGCCCCTC GGGCTTCAGC 
GGCTCCACGT GTCAGCTGGA CGTGGACGAA TGCGCCAGCA CGCCCTGCAG GAATGGCGCC 
AAATGCGTGG ACCAGCCCGA TGGCTACGAG TGCCGCTGTG CCGAGGGCTT TGAGGGCACG 
CTGTGTGATC GCAACGTGGA CGACTGCTCC CCTGACCCAT GCCACCATGG TCGCTGCGTG 
GATGGCATCG CCAGCTTCTC ATGTGCCTGT GCTCCTGGCT ACACGGGCAC ACGCTGCGAG 
AGCCAGGTGG ACGAATGCCG CAGCCAGCCC TGCCGCCATG GCGGCAAATG CCTAGACCTG 
GTGGACAAGT ACCTCTGCCG CTGCCCTTCT GGGACCACAG GTGTGAACTG CGAAGTGAAC 
ATTGACGACT GTGCCAGCAA CCCCTGCACC TTTGGAGTCT GCCGTGATGG CATCAACCGC 
TACGACTGTG TCTGCCAACC TGGCTTCACA GGGCCCCTTT GTAACGTGGA GATCAATGAG 
TGTGCTTCCA GCCCATGCGG CGAGGGAGGT TCCTGTGTGG ATGGGGAAAA TGGCTTCCGC 
TGCCTCTGCC CGCCTGGCTC CTTGCCCCCA CTCTGCCTCC CCCCGAGCCA TCCCTGTGCC 
CATGAGCCCT GCAGTCACGG CATCTGCTAT GATGCACCTG GCGGGTTCCG CTGTGTGTGT 
GAGCCTGGCT GGAGTGGCCC CCGCTGCAGC CAGAGCCTGG CCCGAGACGC CTGTGAGTCC 
CAGCCGTGCA GGGCCGGTGG GACATGCAGC AGCGATGGAA TGGGTTTCCA CTGCACCTGC 
CCGCCTGGTG TCCAGGGACG TCAGTGTGAA CTCCTCTCCC CCTGCACCCC GAACCCCTGT 
GAGCATGGGG GCCGCTGCGA GTCTGCCCCT GGCCAGCTGC CTGTCTGCTC CTGCCCCCAG 
GGCTGGCAAG GCCCACGATG CCAGCAGGAT GTGGACGAGT GTGCTGGCCC CGCACCCTGT 
GGCCCTCATG GTATCTGCAC CAACCTGGCA GGGAGTTTCA GCTGCACCTG CCATGGAGGG 
TACACTGGCC CTTCCTGCGA TCAGGACATC AATGACTGTG ACCCCAACCC ATGCCTGAAC 
GGTGGCTCGT GCCAAGACGG CGTGGGCTCC TTTTCCTGCT CCTGCCTCCC TGGTTTCGCC 
GGCCCACGAT GCGCCCGCGA TGTGGATGAG TGCCTGAGCA ACCCCTGCGG CCCGGGCACC 
TGTACCGACC ACGTGGCCTC CTTCACCTGC ACCTGCCCGC CAGGCTACGG AGGCTTCCAC 
TGCGAACAGG ACCTGCCCGA CTGCAGCCCC AGCTCCTGCT TCAATGGCGG GACCTGTGTG 
GACGGCGTGA ACTCGTTCAG CTGCCTGTGC CGTCCCGGCT ACACAGGAGC CCACTGCCAA 
CATGAGGCAG ACCCCTGCCT CTCGCGGCCC TGCCTACACG GGGGCGTCTG CAGCGCCGCC 
CACCCTGGCT TCCGCTGCAC CTGCCTCGAG AGCTTCACGG GCCCGCAGTG CCAGACGCTG 
GTGGATTGGT GCAGCCGCCA GCCTTGTCAA AACGGGGGTC GCTGCGTCCA GACTGGGGCC 
TATTGCCTTT GTCCCCCTGG ATGGAGCGGA CGCCTCTGTG ACATCCGAAG CTTGCCCTGC 
AGGGAGGCCG CAGCCCAGAT CGGGGTGCGG CTGGAGCAGC TGTGTCAGGC GGGTGGGCAG 
TGTGTGGATG AAGACAGCTC CCACTACTGC GTGTGCCCAG AGGGCCGTAC TGGTAGCCAC 
TGTGAGCAGG AGGTGGACCC CTGCTTGGCC CAGCCCTGCC AGCATGGGGG GACCTGCCGT 
GGCTATATGG GGGGCTACAT GTGTGAGTGT CTTCCTGGCT ACAATGGTGA TAACTGTGAG 
GACGACGTGG ACGAGTGTGC CTCCCAGCCC TGCCAGCACG GGGGTTCATG CATTGACCTC 
GTGGCCCGCT ATCTCTGCTC CTGTCCCCCA GGAACGCTGG GGGTGCTCTG CGAGATTAAT 
GAGGATGACT GCGGCCCAGG CCCACCGCTG GACTCAGGGC CCCGGTGCCT ACACAATGGC 
ACCTGCGTGG ACCTGGTGGG TGGTTTCCGC TGCACCTGTC CCCCAGGATA CACTGGTTTG 
CGCTGCGAGG CAGACATCAA TGAGTGTCGC TCAGGTGCCT GCCACGCGGC ACACACCCGG 
GACTGCCTGC AGGACCCAGG CGGAGGTTTC CGTTGCCTTT GTCATGCTGG CTTCTCAGGT 
CCTCGCTGTC AGACTGTCCT GTCTCCCTGC GAGTCCCAGC CATGCCAGCA TGGAGGCCAG 
TGCCGTCCTA GCCCGGGTCC TGGGGGTGGG CTGACCTTCA CCTGTCACTG TGCCCAGCCG 
TTCTGGGGTC CGCGTTGCGA GCGGGTGGCG CGCTCCTGCC GGGAGCTGCA GTGCCCGGTG 
GGCGTCCCAT GCCAGCAGAC GCCCCGCGGG CCGCGCTGCG CCTGCCCCCC AGGGTTGTCG 
GGACCCTCCT GCCGCAGCTT CCCGGGGTCG CCGCCGGGGG CCAGCAACGC CAGCTGCGCG 
GCCGCCCCCT GTCTCCACGG GGGCTCCTGC CGCCCCGCGC CGCTCGCGCC CTTCTTCCGC 
TGCGCTTGCG CGCAGGGCTG GACCGGGCCG CGCTGCGAGG CGCCCGCCGC GGCACCCGAG 
GTCTCGGAGG AGCCGCGGTG CCCGCGCGCC GCCTGCCAGG CCAAGCGCGG GGACCAGCGC 
TGCGACCGCG AGTGCAACAG CCCAGGCTGC GGCTGGGACG GCGGCGACTG CTCGCTGAGC 
GTGGGCGACC CCTGGCGGCA ATGCGAGGCG CTGCAGTGCT GGCGCCTCTT CAACAACAGC 
CGCTGCGACC CCGCCTGCAG CTCGCCCGCC TGCCTCTACG ACAACTTCGA CTGCCACGCC 
GGTGGCCGCG AGCGCACTTG CAACCCGGTG TACGAGAAGT ACTGCGCCGA CCACTTTGCC 
GACGGCCGCT GCGACCAGGG CTGCAACACG GAGGAGTGCG GCTGGGATGG GCTGGATTGT 
GCCAGCGAGG TGCCGGCCCT GCTGGCCCGC GGCGTGCTGG TGCTCACAGT GCTGCTGCCG 
CCAGAGGAGC TACTGCGTTC CAGCGCCGAC TTTCTGCAGC GGCTCAGCGC CATCCTGCGC 
ACCTCGCTGC GCTTCCGCCT GGACGCGCAC GGCCAGGCCA TGGTCTTCCC TTACCACCGG 
CCTAGTCCTG GCTCCGAACC CCGGGCCCGT CGGGAGCTGG CCCCCGAGGT GATCGGCTCG 
GTAGTAATGC TGGAGATTGA CAACCGGCTC TGCCTGCAGT CGCCTGAGAA TGATCACTGC 
TTCCCCGATG CCCAGAGCGC CGCTGACTAC CTGGGAGCGT TGTCAGCGGT GGAGCGCCTG 
GACTTCCCGT ACCCACTGCG GGACGTGCGG GGGGAGCCGC TGGAGCCTCC AGAACCCAGC 
GTCCCGCTGC TGCCACTGCT AGTGGCGGGC GCTGTCTTGC TGCTGGTCAT TCTCGTCCTG 
GGTGTCATGG TGGCCCGGCG CAAGCGCGAG CACAGCACCC TCTGGTTCCC TGAGGGCTTC 
TCACTGCACA AGGACGTGGC CTCTGGTCAC AAGGGCCGGC GGGAACCCGT GGGCCAGGAC 
GCGCTGGGCA TGAAGAACAT GGCCAAGGGT GAGAGCCTGA TGGGGGAGGT GGCCACAGAC 
TGGATGGACA CAGAGTGCCC AGAGGCCAAG CGGCTAAAGG TAGAGGAGCC AGGCATGGGG 
GCTGAGGAGG CTGTGGATTG CCGTCAGTGG ACTCAACACC ATCTGGTTGC TGCTGACATC 
CGCGTGGCAC CAGCCATGGC ACTGACACCA CCACAGGGCG ACGCAGATGC TGATGGCATG 
GATGTCAATG TGCGTGGCCC AGATGGCTTC ACCCCGCTAA TGCTGGCTTC CTTCTGTGGG 
GGGGCTCTGG AGCCAATGCC AACTGAAGAG GATGAGGCAG ATGACACATC AGCTAGCATC 
ATCTCCGACC TGATCTGCCA GGGGGCTCAG CTTGGGGCAC GGACTGACCG TACTGGCGAG 
ACTGCTTTGC ACCTGGCTGC CCGTTATGCC CGTGCTGATG CAGCCAAGCG GCTGCTGGAT 
GCTGGGGCAG ACACCAATGC CCAGGACCAC TCAGGCCGCA CTCCCCTGCA CACAGCTGTC 
ACAGCCGATG CCCAGGGTGT CTTCCAGATT CTCATCCGAA ACCGCTCTAC AGACTTGGAT 
GCCCGCATGG CAGATGGCTC AACGGCACTG ATCCTGGCGG CCCGCCTGGC AGTAGAGGGC 
ATGGTGGAAG AGCTCATCGC CAGCCATGCT GATGTCAATG CTGTGGATGA GCTTGGGAAA 
TCAGCCTTAC ACTGGGCTGC GGCTGTGAAC AACGTGGAAG CCACTTTGGC CCTGCTCAAA 
AATGGAGCCA ATAAGGACAT GCAGGATAGC AAGGAGGAGA CCCCCCTATT CCTGGCCGCC 
CGCGAGGGCA GCTATGAGGC TGCCAAGCTG CTGTTGGACC ACTTTGCCAA CCGTGAGATC 
ACCGACCACC TGGACAGGCT GCCGCGGGAC GTAGCCCAGG AGAGACTGCA CCAGGACATC 
GTGCGCTTGC TGGATCAACC CAGTGGGCCC CGCAGCCCCC CCGGTCCCCA CGGCCTGGGG 
CCTCTGCTCT GTCCTCCAGG GGCCTTCCTC CCTGGCCTCA AAGCGGCACA GTCGGGGTCC 
AAGAAGAGCA GGAGGCCCCC CGGGAAGGCG GGGCTGGGGC CGCAGGGGCC CCGGGGGCGG 
GGCAAGAAGC TGACGCTGGC CTGCCCGGGC CCCCTGGCTG ACAGCTCGGT CACGCTGTCG 
CCCGTGGACT CGCTGGACTC CCCGCGGCCT TTCGGTGGGC CCCCTGCTTC CCCTGGTGGC 
TTCCCCCTTG AGGGGCCCTA TGCAGCTGCC ACTGCCACTG CAGTGTCTCT GGCACAGCTT 
GGTGGCCCAG GCCGGGCGGG TCTAGGGCGC CAGCCCCCTG GAGGATGTGT ACTCAGCCTG 
GGCCTGCTGA ACCCTGTGGC TGTGCCCCTC GATTGGGCCC GGCTGCCCCC ACCTGCCCCT 
CCAGGCCCCT CGTTCCTGCT GCCACTGGCG CCGGGACCCC AGCTGCTCAA CCCAGGGACC 
CCCGTCTCCC CGCAGGAGCG GCCCCCGCCT TACCTGGCAG TCCCAGGACA TGGCGAGGAG 
TACCCGGCGG CTGGGGCACA CAGCAGCCCC CCAAAGGCCC GCTTCCTGCG GGTTCCCAGT 
GAGCACCCTT ACCTGACCCC ATCCCCCGAA TCCCCTGAGC ACTGGGCCAG CCCCTCACCT 
CCCTCCCTCT CAGACTGGTC CGAATCCACG CCTAGCCCAG CCACTGCCAC TGGGGCCATG 
GCCACCACCA CTGGGGCACT GCCTGCCCAG CCACTTCCCT TGTCTGTTCC CAGCTCCCTT 
GCTCAGGCCC AGACCCAGCT GGGGCCCCAG CCGGAAGTTA CCCCCAAGAG GCAAGTGTTG 
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24165
Clone ID Related Accession (Same CDS sequence) NM_000435.3 , NM_000435.2
Accession Version NM_000435.2 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 6966bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2017-12-05
Organism Homo sapiens(human)
Product neurogenic locus notch homolog protein 3 precursor
Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U97669.1 and AC004663.1. This sequence is a reference standard in the RefSeqGene project. On Mar 28, 2007 this sequence version replaced NM_000435.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U97669.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
ATGGGGCCGG GGGCCCGTGG CCGCCGCCGC CGCCGTCGCC CGATGTCGCC GCCACCGCCA 
CCGCCACCCG TGCGGGCGCT GCCCCTGCTG CTGCTGCTAG CGGGGCCGGG GGCTGCAGCC 
CCCCCTTGCC TGGACGGAAG CCCGTGTGCA AATGGAGGTC GTTGCACCCA GCTGCCCTCC 
CGGGAGGCTG CCTGCCTGTG CCCGCCTGGC TGGGTGGGTG AGCGGTGTCA GCTGGAGGAC 
CCCTGTCACT CAGGCCCCTG TGCTGGCCGT GGTGTCTGCC AGAGTTCAGT GGTGGCTGGC 
ACCGCCCGAT TCTCATGCCG GTGCCCCCGT GGCTTCCGAG GCCCTGACTG CTCCCTGCCA 
GATCCCTGCC TCAGCAGCCC TTGTGCCCAC GGTGCCCGCT GCTCAGTGGG GCCCGATGGA 
CGCTTCCTCT GCTCCTGCCC ACCTGGCTAC CAGGGCCGCA GCTGCCGAAG CGACGTGGAT 
GAGTGCCGGG TGGGTGAGCC CTGCCGCCAT GGTGGCACCT GCCTCAACAC ACCTGGCTCC 
TTCCGCTGCC AGTGTCCAGC TGGCTACACA GGGCCACTAT GTGAGAACCC CGCGGTGCCC 
TGTGCACCCT CACCATGCCG TAACGGGGGC ACCTGCAGGC AGAGTGGCGA CCTCACTTAC 
GACTGTGCCT GTCTTCCTGG GTTTGAGGGT CAGAATTGTG AAGTGAACGT GGACGACTGT 
CCAGGACACC GATGTCTCAA TGGGGGGACA TGCGTGGATG GCGTCAACAC CTATAACTGC 
CAGTGCCCTC CTGAGTGGAC AGGCCAGTTC TGCACGGAGG ACGTGGATGA GTGTCAGCTG 
CAGCCCAACG CCTGCCACAA TGGGGGTACC TGCTTCAACA CGCTGGGTGG CCACAGCTGC 
GTGTGTGTCA ATGGCTGGAC AGGCGAGAGC TGCAGTCAGA ATATCGATGA CTGTGCCACA 
GCCGTGTGCT TCCATGGGGC CACCTGCCAT GACCGCGTGG CTTCTTTCTA CTGTGCCTGC 
CCCATGGGCA AGACTGGCCT CCTGTGTCAC CTGGATGACG CCTGTGTCAG CAACCCCTGC 
CACGAGGATG CTATCTGTGA CACAAATCCG GTGAACGGCC GGGCCATTTG CACCTGTCCT 
CCCGGCTTCA CGGGTGGGGC ATGTGACCAG GATGTGGACG AGTGCTCTAT CGGCGCCAAC 
CCCTGCGAGC ACTTGGGCAG GTGCGTGAAC ACGCAGGGCT CCTTCCTGTG CCAGTGCGGT 
CGTGGCTACA CTGGACCTCG CTGTGAGACC GATGTCAACG AGTGTCTGTC GGGGCCCTGC 
CGAAACCAGG CCACGTGCCT CGACCGCATA GGCCAGTTCA CCTGTATCTG TATGGCAGGC 
TTCACAGGAA CCTATTGCGA GGTGGACATT GACGAGTGTC AGAGTAGCCC CTGTGTCAAC 
GGTGGGGTCT GCAAGGACCG AGTCAATGGC TTCAGCTGCA CCTGCCCCTC GGGCTTCAGC 
GGCTCCACGT GTCAGCTGGA CGTGGACGAA TGCGCCAGCA CGCCCTGCAG GAATGGCGCC 
AAATGCGTGG ACCAGCCCGA TGGCTACGAG TGCCGCTGTG CCGAGGGCTT TGAGGGCACG 
CTGTGTGATC GCAACGTGGA CGACTGCTCC CCTGACCCAT GCCACCATGG TCGCTGCGTG 
GATGGCATCG CCAGCTTCTC ATGTGCCTGT GCTCCTGGCT ACACGGGCAC ACGCTGCGAG 
AGCCAGGTGG ACGAATGCCG CAGCCAGCCC TGCCGCCATG GCGGCAAATG CCTAGACCTG 
GTGGACAAGT ACCTCTGCCG CTGCCCTTCT GGGACCACAG GTGTGAACTG CGAAGTGAAC 
ATTGACGACT GTGCCAGCAA CCCCTGCACC TTTGGAGTCT GCCGTGATGG CATCAACCGC 
TACGACTGTG TCTGCCAACC TGGCTTCACA GGGCCCCTTT GTAACGTGGA GATCAATGAG 
TGTGCTTCCA GCCCATGCGG CGAGGGAGGT TCCTGTGTGG ATGGGGAAAA TGGCTTCCGC 
TGCCTCTGCC CGCCTGGCTC CTTGCCCCCA CTCTGCCTCC CCCCGAGCCA TCCCTGTGCC 
CATGAGCCCT GCAGTCACGG CATCTGCTAT GATGCACCTG GCGGGTTCCG CTGTGTGTGT 
GAGCCTGGCT GGAGTGGCCC CCGCTGCAGC CAGAGCCTGG CCCGAGACGC CTGTGAGTCC 
CAGCCGTGCA GGGCCGGTGG GACATGCAGC AGCGATGGAA TGGGTTTCCA CTGCACCTGC 
CCGCCTGGTG TCCAGGGACG TCAGTGTGAA CTCCTCTCCC CCTGCACCCC GAACCCCTGT 
GAGCATGGGG GCCGCTGCGA GTCTGCCCCT GGCCAGCTGC CTGTCTGCTC CTGCCCCCAG 
GGCTGGCAAG GCCCACGATG CCAGCAGGAT GTGGACGAGT GTGCTGGCCC CGCACCCTGT 
GGCCCTCATG GTATCTGCAC CAACCTGGCA GGGAGTTTCA GCTGCACCTG CCATGGAGGG 
TACACTGGCC CTTCCTGCGA TCAGGACATC AATGACTGTG ACCCCAACCC ATGCCTGAAC 
GGTGGCTCGT GCCAAGACGG CGTGGGCTCC TTTTCCTGCT CCTGCCTCCC TGGTTTCGCC 
GGCCCACGAT GCGCCCGCGA TGTGGATGAG TGCCTGAGCA ACCCCTGCGG CCCGGGCACC 
TGTACCGACC ACGTGGCCTC CTTCACCTGC ACCTGCCCGC CAGGCTACGG AGGCTTCCAC 
TGCGAACAGG ACCTGCCCGA CTGCAGCCCC AGCTCCTGCT TCAATGGCGG GACCTGTGTG 
GACGGCGTGA ACTCGTTCAG CTGCCTGTGC CGTCCCGGCT ACACAGGAGC CCACTGCCAA 
CATGAGGCAG ACCCCTGCCT CTCGCGGCCC TGCCTACACG GGGGCGTCTG CAGCGCCGCC 
CACCCTGGCT TCCGCTGCAC CTGCCTCGAG AGCTTCACGG GCCCGCAGTG CCAGACGCTG 
GTGGATTGGT GCAGCCGCCA GCCTTGTCAA AACGGGGGTC GCTGCGTCCA GACTGGGGCC 
TATTGCCTTT GTCCCCCTGG ATGGAGCGGA CGCCTCTGTG ACATCCGAAG CTTGCCCTGC 
AGGGAGGCCG CAGCCCAGAT CGGGGTGCGG CTGGAGCAGC TGTGTCAGGC GGGTGGGCAG 
TGTGTGGATG AAGACAGCTC CCACTACTGC GTGTGCCCAG AGGGCCGTAC TGGTAGCCAC 
TGTGAGCAGG AGGTGGACCC CTGCTTGGCC CAGCCCTGCC AGCATGGGGG GACCTGCCGT 
GGCTATATGG GGGGCTACAT GTGTGAGTGT CTTCCTGGCT ACAATGGTGA TAACTGTGAG 
GACGACGTGG ACGAGTGTGC CTCCCAGCCC TGCCAGCACG GGGGTTCATG CATTGACCTC 
GTGGCCCGCT ATCTCTGCTC CTGTCCCCCA GGAACGCTGG GGGTGCTCTG CGAGATTAAT 
GAGGATGACT GCGGCCCAGG CCCACCGCTG GACTCAGGGC CCCGGTGCCT ACACAATGGC 
ACCTGCGTGG ACCTGGTGGG TGGTTTCCGC TGCACCTGTC CCCCAGGATA CACTGGTTTG 
CGCTGCGAGG CAGACATCAA TGAGTGTCGC TCAGGTGCCT GCCACGCGGC ACACACCCGG 
GACTGCCTGC AGGACCCAGG CGGAGGTTTC CGTTGCCTTT GTCATGCTGG CTTCTCAGGT 
CCTCGCTGTC AGACTGTCCT GTCTCCCTGC GAGTCCCAGC CATGCCAGCA TGGAGGCCAG 
TGCCGTCCTA GCCCGGGTCC TGGGGGTGGG CTGACCTTCA CCTGTCACTG TGCCCAGCCG 
TTCTGGGGTC CGCGTTGCGA GCGGGTGGCG CGCTCCTGCC GGGAGCTGCA GTGCCCGGTG 
GGCGTCCCAT GCCAGCAGAC GCCCCGCGGG CCGCGCTGCG CCTGCCCCCC AGGGTTGTCG 
GGACCCTCCT GCCGCAGCTT CCCGGGGTCG CCGCCGGGGG CCAGCAACGC CAGCTGCGCG 
GCCGCCCCCT GTCTCCACGG GGGCTCCTGC CGCCCCGCGC CGCTCGCGCC CTTCTTCCGC 
TGCGCTTGCG CGCAGGGCTG GACCGGGCCG CGCTGCGAGG CGCCCGCCGC GGCACCCGAG 
GTCTCGGAGG AGCCGCGGTG CCCGCGCGCC GCCTGCCAGG CCAAGCGCGG GGACCAGCGC 
TGCGACCGCG AGTGCAACAG CCCAGGCTGC GGCTGGGACG GCGGCGACTG CTCGCTGAGC 
GTGGGCGACC CCTGGCGGCA ATGCGAGGCG CTGCAGTGCT GGCGCCTCTT CAACAACAGC 
CGCTGCGACC CCGCCTGCAG CTCGCCCGCC TGCCTCTACG ACAACTTCGA CTGCCACGCC 
GGTGGCCGCG AGCGCACTTG CAACCCGGTG TACGAGAAGT ACTGCGCCGA CCACTTTGCC 
GACGGCCGCT GCGACCAGGG CTGCAACACG GAGGAGTGCG GCTGGGATGG GCTGGATTGT 
GCCAGCGAGG TGCCGGCCCT GCTGGCCCGC GGCGTGCTGG TGCTCACAGT GCTGCTGCCG 
CCAGAGGAGC TACTGCGTTC CAGCGCCGAC TTTCTGCAGC GGCTCAGCGC CATCCTGCGC 
ACCTCGCTGC GCTTCCGCCT GGACGCGCAC GGCCAGGCCA TGGTCTTCCC TTACCACCGG 
CCTAGTCCTG GCTCCGAACC CCGGGCCCGT CGGGAGCTGG CCCCCGAGGT GATCGGCTCG 
GTAGTAATGC TGGAGATTGA CAACCGGCTC TGCCTGCAGT CGCCTGAGAA TGATCACTGC 
TTCCCCGATG CCCAGAGCGC CGCTGACTAC CTGGGAGCGT TGTCAGCGGT GGAGCGCCTG 
GACTTCCCGT ACCCACTGCG GGACGTGCGG GGGGAGCCGC TGGAGCCTCC AGAACCCAGC 
GTCCCGCTGC TGCCACTGCT AGTGGCGGGC GCTGTCTTGC TGCTGGTCAT TCTCGTCCTG 
GGTGTCATGG TGGCCCGGCG CAAGCGCGAG CACAGCACCC TCTGGTTCCC TGAGGGCTTC 
TCACTGCACA AGGACGTGGC CTCTGGTCAC AAGGGCCGGC GGGAACCCGT GGGCCAGGAC 
GCGCTGGGCA TGAAGAACAT GGCCAAGGGT GAGAGCCTGA TGGGGGAGGT GGCCACAGAC 
TGGATGGACA CAGAGTGCCC AGAGGCCAAG CGGCTAAAGG TAGAGGAGCC AGGCATGGGG 
GCTGAGGAGG CTGTGGATTG CCGTCAGTGG ACTCAACACC ATCTGGTTGC TGCTGACATC 
CGCGTGGCAC CAGCCATGGC ACTGACACCA CCACAGGGCG ACGCAGATGC TGATGGCATG 
GATGTCAATG TGCGTGGCCC AGATGGCTTC ACCCCGCTAA TGCTGGCTTC CTTCTGTGGG 
GGGGCTCTGG AGCCAATGCC AACTGAAGAG GATGAGGCAG ATGACACATC AGCTAGCATC 
ATCTCCGACC TGATCTGCCA GGGGGCTCAG CTTGGGGCAC GGACTGACCG TACTGGCGAG 
ACTGCTTTGC ACCTGGCTGC CCGTTATGCC CGTGCTGATG CAGCCAAGCG GCTGCTGGAT 
GCTGGGGCAG ACACCAATGC CCAGGACCAC TCAGGCCGCA CTCCCCTGCA CACAGCTGTC 
ACAGCCGATG CCCAGGGTGT CTTCCAGATT CTCATCCGAA ACCGCTCTAC AGACTTGGAT 
GCCCGCATGG CAGATGGCTC AACGGCACTG ATCCTGGCGG CCCGCCTGGC AGTAGAGGGC 
ATGGTGGAAG AGCTCATCGC CAGCCATGCT GATGTCAATG CTGTGGATGA GCTTGGGAAA 
TCAGCCTTAC ACTGGGCTGC GGCTGTGAAC AACGTGGAAG CCACTTTGGC CCTGCTCAAA 
AATGGAGCCA ATAAGGACAT GCAGGATAGC AAGGAGGAGA CCCCCCTATT CCTGGCCGCC 
CGCGAGGGCA GCTATGAGGC TGCCAAGCTG CTGTTGGACC ACTTTGCCAA CCGTGAGATC 
ACCGACCACC TGGACAGGCT GCCGCGGGAC GTAGCCCAGG AGAGACTGCA CCAGGACATC 
GTGCGCTTGC TGGATCAACC CAGTGGGCCC CGCAGCCCCC CCGGTCCCCA CGGCCTGGGG 
CCTCTGCTCT GTCCTCCAGG GGCCTTCCTC CCTGGCCTCA AAGCGGCACA GTCGGGGTCC 
AAGAAGAGCA GGAGGCCCCC CGGGAAGGCG GGGCTGGGGC CGCAGGGGCC CCGGGGGCGG 
GGCAAGAAGC TGACGCTGGC CTGCCCGGGC CCCCTGGCTG ACAGCTCGGT CACGCTGTCG 
CCCGTGGACT CGCTGGACTC CCCGCGGCCT TTCGGTGGGC CCCCTGCTTC CCCTGGTGGC 
TTCCCCCTTG AGGGGCCCTA TGCAGCTGCC ACTGCCACTG CAGTGTCTCT GGCACAGCTT 
GGTGGCCCAG GCCGGGCGGG TCTAGGGCGC CAGCCCCCTG GAGGATGTGT ACTCAGCCTG 
GGCCTGCTGA ACCCTGTGGC TGTGCCCCTC GATTGGGCCC GGCTGCCCCC ACCTGCCCCT 
CCAGGCCCCT CGTTCCTGCT GCCACTGGCG CCGGGACCCC AGCTGCTCAA CCCAGGGACC 
CCCGTCTCCC CGCAGGAGCG GCCCCCGCCT TACCTGGCAG TCCCAGGACA TGGCGAGGAG 
TACCCGGCGG CTGGGGCACA CAGCAGCCCC CCAAAGGCCC GCTTCCTGCG GGTTCCCAGT 
GAGCACCCTT ACCTGACCCC ATCCCCCGAA TCCCCTGAGC ACTGGGCCAG CCCCTCACCT 
CCCTCCCTCT CAGACTGGTC CGAATCCACG CCTAGCCCAG CCACTGCCAC TGGGGCCATG 
GCCACCACCA CTGGGGCACT GCCTGCCCAG CCACTTCCCT TGTCTGTTCC CAGCTCCCTT 
GCTCAGGCCC AGACCCAGCT GGGGCCCCAG CCGGAAGTTA CCCCCAAGAG GCAAGTGTTG 
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000426.2
CDS77..7042
Translation

Target ORF information:

RefSeq Version NM_000435.2
Organism Homo sapiens(human)
Definition Homo sapiens notch 3 (NOTCH3), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_000435.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
6841
6901
6961
ATGGGGCCGG GGGCCCGTGG CCGCCGCCGC CGCCGTCGCC CGATGTCGCC GCCACCGCCA 
CCGCCACCCG TGCGGGCGCT GCCCCTGCTG CTGCTGCTAG CGGGGCCGGG GGCTGCAGCC 
CCCCCTTGCC TGGACGGAAG CCCGTGTGCA AATGGAGGTC GTTGCACCCA GCTGCCCTCC 
CGGGAGGCTG CCTGCCTGTG CCCGCCTGGC TGGGTGGGTG AGCGGTGTCA GCTGGAGGAC 
CCCTGTCACT CAGGCCCCTG TGCTGGCCGT GGTGTCTGCC AGAGTTCAGT GGTGGCTGGC 
ACCGCCCGAT TCTCATGCCG GTGCCCCCGT GGCTTCCGAG GCCCTGACTG CTCCCTGCCA 
GATCCCTGCC TCAGCAGCCC TTGTGCCCAC GGTGCCCGCT GCTCAGTGGG GCCCGATGGA 
CGCTTCCTCT GCTCCTGCCC ACCTGGCTAC CAGGGCCGCA GCTGCCGAAG CGACGTGGAT 
GAGTGCCGGG TGGGTGAGCC CTGCCGCCAT GGTGGCACCT GCCTCAACAC ACCTGGCTCC 
TTCCGCTGCC AGTGTCCAGC TGGCTACACA GGGCCACTAT GTGAGAACCC CGCGGTGCCC 
TGTGCACCCT CACCATGCCG TAACGGGGGC ACCTGCAGGC AGAGTGGCGA CCTCACTTAC 
GACTGTGCCT GTCTTCCTGG GTTTGAGGGT CAGAATTGTG AAGTGAACGT GGACGACTGT 
CCAGGACACC GATGTCTCAA TGGGGGGACA TGCGTGGATG GCGTCAACAC CTATAACTGC 
CAGTGCCCTC CTGAGTGGAC AGGCCAGTTC TGCACGGAGG ACGTGGATGA GTGTCAGCTG 
CAGCCCAACG CCTGCCACAA TGGGGGTACC TGCTTCAACA CGCTGGGTGG CCACAGCTGC 
GTGTGTGTCA ATGGCTGGAC AGGCGAGAGC TGCAGTCAGA ATATCGATGA CTGTGCCACA 
GCCGTGTGCT TCCATGGGGC CACCTGCCAT GACCGCGTGG CTTCTTTCTA CTGTGCCTGC 
CCCATGGGCA AGACTGGCCT CCTGTGTCAC CTGGATGACG CCTGTGTCAG CAACCCCTGC 
CACGAGGATG CTATCTGTGA CACAAATCCG GTGAACGGCC GGGCCATTTG CACCTGTCCT 
CCCGGCTTCA CGGGTGGGGC ATGTGACCAG GATGTGGACG AGTGCTCTAT CGGCGCCAAC 
CCCTGCGAGC ACTTGGGCAG GTGCGTGAAC ACGCAGGGCT CCTTCCTGTG CCAGTGCGGT 
CGTGGCTACA CTGGACCTCG CTGTGAGACC GATGTCAACG AGTGTCTGTC GGGGCCCTGC 
CGAAACCAGG CCACGTGCCT CGACCGCATA GGCCAGTTCA CCTGTATCTG TATGGCAGGC 
TTCACAGGAA CCTATTGCGA GGTGGACATT GACGAGTGTC AGAGTAGCCC CTGTGTCAAC 
GGTGGGGTCT GCAAGGACCG AGTCAATGGC TTCAGCTGCA CCTGCCCCTC GGGCTTCAGC 
GGCTCCACGT GTCAGCTGGA CGTGGACGAA TGCGCCAGCA CGCCCTGCAG GAATGGCGCC 
AAATGCGTGG ACCAGCCCGA TGGCTACGAG TGCCGCTGTG CCGAGGGCTT TGAGGGCACG 
CTGTGTGATC GCAACGTGGA CGACTGCTCC CCTGACCCAT GCCACCATGG TCGCTGCGTG 
GATGGCATCG CCAGCTTCTC ATGTGCCTGT GCTCCTGGCT ACACGGGCAC ACGCTGCGAG 
AGCCAGGTGG ACGAATGCCG CAGCCAGCCC TGCCGCCATG GCGGCAAATG CCTAGACCTG 
GTGGACAAGT ACCTCTGCCG CTGCCCTTCT GGGACCACAG GTGTGAACTG CGAAGTGAAC 
ATTGACGACT GTGCCAGCAA CCCCTGCACC TTTGGAGTCT GCCGTGATGG CATCAACCGC 
TACGACTGTG TCTGCCAACC TGGCTTCACA GGGCCCCTTT GTAACGTGGA GATCAATGAG 
TGTGCTTCCA GCCCATGCGG CGAGGGAGGT TCCTGTGTGG ATGGGGAAAA TGGCTTCCGC 
TGCCTCTGCC CGCCTGGCTC CTTGCCCCCA CTCTGCCTCC CCCCGAGCCA TCCCTGTGCC 
CATGAGCCCT GCAGTCACGG CATCTGCTAT GATGCACCTG GCGGGTTCCG CTGTGTGTGT 
GAGCCTGGCT GGAGTGGCCC CCGCTGCAGC CAGAGCCTGG CCCGAGACGC CTGTGAGTCC 
CAGCCGTGCA GGGCCGGTGG GACATGCAGC AGCGATGGAA TGGGTTTCCA CTGCACCTGC 
CCGCCTGGTG TCCAGGGACG TCAGTGTGAA CTCCTCTCCC CCTGCACCCC GAACCCCTGT 
GAGCATGGGG GCCGCTGCGA GTCTGCCCCT GGCCAGCTGC CTGTCTGCTC CTGCCCCCAG 
GGCTGGCAAG GCCCACGATG CCAGCAGGAT GTGGACGAGT GTGCTGGCCC CGCACCCTGT 
GGCCCTCATG GTATCTGCAC CAACCTGGCA GGGAGTTTCA GCTGCACCTG CCATGGAGGG 
TACACTGGCC CTTCCTGCGA TCAGGACATC AATGACTGTG ACCCCAACCC ATGCCTGAAC 
GGTGGCTCGT GCCAAGACGG CGTGGGCTCC TTTTCCTGCT CCTGCCTCCC TGGTTTCGCC 
GGCCCACGAT GCGCCCGCGA TGTGGATGAG TGCCTGAGCA ACCCCTGCGG CCCGGGCACC 
TGTACCGACC ACGTGGCCTC CTTCACCTGC ACCTGCCCGC CAGGCTACGG AGGCTTCCAC 
TGCGAACAGG ACCTGCCCGA CTGCAGCCCC AGCTCCTGCT TCAATGGCGG GACCTGTGTG 
GACGGCGTGA ACTCGTTCAG CTGCCTGTGC CGTCCCGGCT ACACAGGAGC CCACTGCCAA 
CATGAGGCAG ACCCCTGCCT CTCGCGGCCC TGCCTACACG GGGGCGTCTG CAGCGCCGCC 
CACCCTGGCT TCCGCTGCAC CTGCCTCGAG AGCTTCACGG GCCCGCAGTG CCAGACGCTG 
GTGGATTGGT GCAGCCGCCA GCCTTGTCAA AACGGGGGTC GCTGCGTCCA GACTGGGGCC 
TATTGCCTTT GTCCCCCTGG ATGGAGCGGA CGCCTCTGTG ACATCCGAAG CTTGCCCTGC 
AGGGAGGCCG CAGCCCAGAT CGGGGTGCGG CTGGAGCAGC TGTGTCAGGC GGGTGGGCAG 
TGTGTGGATG AAGACAGCTC CCACTACTGC GTGTGCCCAG AGGGCCGTAC TGGTAGCCAC 
TGTGAGCAGG AGGTGGACCC CTGCTTGGCC CAGCCCTGCC AGCATGGGGG GACCTGCCGT 
GGCTATATGG GGGGCTACAT GTGTGAGTGT CTTCCTGGCT ACAATGGTGA TAACTGTGAG 
GACGACGTGG ACGAGTGTGC CTCCCAGCCC TGCCAGCACG GGGGTTCATG CATTGACCTC 
GTGGCCCGCT ATCTCTGCTC CTGTCCCCCA GGAACGCTGG GGGTGCTCTG CGAGATTAAT 
GAGGATGACT GCGGCCCAGG CCCACCGCTG GACTCAGGGC CCCGGTGCCT ACACAATGGC 
ACCTGCGTGG ACCTGGTGGG TGGTTTCCGC TGCACCTGTC CCCCAGGATA CACTGGTTTG 
CGCTGCGAGG CAGACATCAA TGAGTGTCGC TCAGGTGCCT GCCACGCGGC ACACACCCGG 
GACTGCCTGC AGGACCCAGG CGGAGGTTTC CGTTGCCTTT GTCATGCTGG CTTCTCAGGT 
CCTCGCTGTC AGACTGTCCT GTCTCCCTGC GAGTCCCAGC CATGCCAGCA TGGAGGCCAG 
TGCCGTCCTA GCCCGGGTCC TGGGGGTGGG CTGACCTTCA CCTGTCACTG TGCCCAGCCG 
TTCTGGGGTC CGCGTTGCGA GCGGGTGGCG CGCTCCTGCC GGGAGCTGCA GTGCCCGGTG 
GGCGTCCCAT GCCAGCAGAC GCCCCGCGGG CCGCGCTGCG CCTGCCCCCC AGGGTTGTCG 
GGACCCTCCT GCCGCAGCTT CCCGGGGTCG CCGCCGGGGG CCAGCAACGC CAGCTGCGCG 
GCCGCCCCCT GTCTCCACGG GGGCTCCTGC CGCCCCGCGC CGCTCGCGCC CTTCTTCCGC 
TGCGCTTGCG CGCAGGGCTG GACCGGGCCG CGCTGCGAGG CGCCCGCCGC GGCACCCGAG 
GTCTCGGAGG AGCCGCGGTG CCCGCGCGCC GCCTGCCAGG CCAAGCGCGG GGACCAGCGC 
TGCGACCGCG AGTGCAACAG CCCAGGCTGC GGCTGGGACG GCGGCGACTG CTCGCTGAGC 
GTGGGCGACC CCTGGCGGCA ATGCGAGGCG CTGCAGTGCT GGCGCCTCTT CAACAACAGC 
CGCTGCGACC CCGCCTGCAG CTCGCCCGCC TGCCTCTACG ACAACTTCGA CTGCCACGCC 
GGTGGCCGCG AGCGCACTTG CAACCCGGTG TACGAGAAGT ACTGCGCCGA CCACTTTGCC 
GACGGCCGCT GCGACCAGGG CTGCAACACG GAGGAGTGCG GCTGGGATGG GCTGGATTGT 
GCCAGCGAGG TGCCGGCCCT GCTGGCCCGC GGCGTGCTGG TGCTCACAGT GCTGCTGCCG 
CCAGAGGAGC TACTGCGTTC CAGCGCCGAC TTTCTGCAGC GGCTCAGCGC CATCCTGCGC 
ACCTCGCTGC GCTTCCGCCT GGACGCGCAC GGCCAGGCCA TGGTCTTCCC TTACCACCGG 
CCTAGTCCTG GCTCCGAACC CCGGGCCCGT CGGGAGCTGG CCCCCGAGGT GATCGGCTCG 
GTAGTAATGC TGGAGATTGA CAACCGGCTC TGCCTGCAGT CGCCTGAGAA TGATCACTGC 
TTCCCCGATG CCCAGAGCGC CGCTGACTAC CTGGGAGCGT TGTCAGCGGT GGAGCGCCTG 
GACTTCCCGT ACCCACTGCG GGACGTGCGG GGGGAGCCGC TGGAGCCTCC AGAACCCAGC 
GTCCCGCTGC TGCCACTGCT AGTGGCGGGC GCTGTCTTGC TGCTGGTCAT TCTCGTCCTG 
GGTGTCATGG TGGCCCGGCG CAAGCGCGAG CACAGCACCC TCTGGTTCCC TGAGGGCTTC 
TCACTGCACA AGGACGTGGC CTCTGGTCAC AAGGGCCGGC GGGAACCCGT GGGCCAGGAC 
GCGCTGGGCA TGAAGAACAT GGCCAAGGGT GAGAGCCTGA TGGGGGAGGT GGCCACAGAC 
TGGATGGACA CAGAGTGCCC AGAGGCCAAG CGGCTAAAGG TAGAGGAGCC AGGCATGGGG 
GCTGAGGAGG CTGTGGATTG CCGTCAGTGG ACTCAACACC ATCTGGTTGC TGCTGACATC 
CGCGTGGCAC CAGCCATGGC ACTGACACCA CCACAGGGCG ACGCAGATGC TGATGGCATG 
GATGTCAATG TGCGTGGCCC AGATGGCTTC ACCCCGCTAA TGCTGGCTTC CTTCTGTGGG 
GGGGCTCTGG AGCCAATGCC AACTGAAGAG GATGAGGCAG ATGACACATC AGCTAGCATC 
ATCTCCGACC TGATCTGCCA GGGGGCTCAG CTTGGGGCAC GGACTGACCG TACTGGCGAG 
ACTGCTTTGC ACCTGGCTGC CCGTTATGCC CGTGCTGATG CAGCCAAGCG GCTGCTGGAT 
GCTGGGGCAG ACACCAATGC CCAGGACCAC TCAGGCCGCA CTCCCCTGCA CACAGCTGTC 
ACAGCCGATG CCCAGGGTGT CTTCCAGATT CTCATCCGAA ACCGCTCTAC AGACTTGGAT 
GCCCGCATGG CAGATGGCTC AACGGCACTG ATCCTGGCGG CCCGCCTGGC AGTAGAGGGC 
ATGGTGGAAG AGCTCATCGC CAGCCATGCT GATGTCAATG CTGTGGATGA GCTTGGGAAA 
TCAGCCTTAC ACTGGGCTGC GGCTGTGAAC AACGTGGAAG CCACTTTGGC CCTGCTCAAA 
AATGGAGCCA ATAAGGACAT GCAGGATAGC AAGGAGGAGA CCCCCCTATT CCTGGCCGCC 
CGCGAGGGCA GCTATGAGGC TGCCAAGCTG CTGTTGGACC ACTTTGCCAA CCGTGAGATC 
ACCGACCACC TGGACAGGCT GCCGCGGGAC GTAGCCCAGG AGAGACTGCA CCAGGACATC 
GTGCGCTTGC TGGATCAACC CAGTGGGCCC CGCAGCCCCC CCGGTCCCCA CGGCCTGGGG 
CCTCTGCTCT GTCCTCCAGG GGCCTTCCTC CCTGGCCTCA AAGCGGCACA GTCGGGGTCC 
AAGAAGAGCA GGAGGCCCCC CGGGAAGGCG GGGCTGGGGC CGCAGGGGCC CCGGGGGCGG 
GGCAAGAAGC TGACGCTGGC CTGCCCGGGC CCCCTGGCTG ACAGCTCGGT CACGCTGTCG 
CCCGTGGACT CGCTGGACTC CCCGCGGCCT TTCGGTGGGC CCCCTGCTTC CCCTGGTGGC 
TTCCCCCTTG AGGGGCCCTA TGCAGCTGCC ACTGCCACTG CAGTGTCTCT GGCACAGCTT 
GGTGGCCCAG GCCGGGCGGG TCTAGGGCGC CAGCCCCCTG GAGGATGTGT ACTCAGCCTG 
GGCCTGCTGA ACCCTGTGGC TGTGCCCCTC GATTGGGCCC GGCTGCCCCC ACCTGCCCCT 
CCAGGCCCCT CGTTCCTGCT GCCACTGGCG CCGGGACCCC AGCTGCTCAA CCCAGGGACC 
CCCGTCTCCC CGCAGGAGCG GCCCCCGCCT TACCTGGCAG TCCCAGGACA TGGCGAGGAG 
TACCCGGCGG CTGGGGCACA CAGCAGCCCC CCAAAGGCCC GCTTCCTGCG GGTTCCCAGT 
GAGCACCCTT ACCTGACCCC ATCCCCCGAA TCCCCTGAGC ACTGGGCCAG CCCCTCACCT 
CCCTCCCTCT CAGACTGGTC CGAATCCACG CCTAGCCCAG CCACTGCCAC TGGGGCCATG 
GCCACCACCA CTGGGGCACT GCCTGCCCAG CCACTTCCCT TGTCTGTTCC CAGCTCCCTT 
GCTCAGGCCC AGACCCAGCT GGGGCCCCAG CCGGAAGTTA CCCCCAAGAG GCAAGTGTTG 
GCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51627
Clone ID Related Accession (Same CDS sequence) XM_005259924.4
Accession Version XM_005259924.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 6810bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product neurogenic locus notch homolog protein 3 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005259924.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
ATGGGGCCGG GGGCCCGTGG CCGCCGCCGC CGCCGTCGCC CGATGTCGCC GCCACCGCCA 
CCGCCACCCG TGCGGGCGCT GCCCCTGCTG CTGCTGCTAG CGGGGCCGGG GGCTGCAGCC 
CCCCCTTGCC TGGACGGAAG CCCGTGTGCA AATGGAGGTC GTTGCACCCA GCTGCCCTCC 
CGGGAGGCTG CCTGCCTGTG CCCGCCTGGC TGGGTGGGTG AGCGGTGTCA GCTGGAGGAC 
CCCTGTCACT CAGGCCCCTG TGCTGGCCGT GGTGTCTGCC AGAGTTCAGT GGTGGCTGGC 
ACCGCCCGAT TCTCATGCCG GTGCCCCCGT GGCTTCCGAG GCCCTGACTG CTCCCTGCCA 
GATCCCTGCC TCAGCAGCCC TTGTGCCCAC GGTGCCCGCT GCTCAGTGGG GCCCGATGGA 
CGCTTCCTCT GCTCCTGCCC ACCTGGCTAC CAGGGCCGCA GCTGCCGAAG CGACGTGGAT 
GAGTGCCGGG TGGGTGAGCC CTGCCGCCAT GGTGGCACCT GCCTCAACAC ACCTGGCTCC 
TTCCGCTGCC AGTGTCCAGC TGGCTACACA GGGCCACTAT GTGAGAACCC CGCGGTGCCC 
TGTGCACCCT CACCATGCCG TAACGGGGGC ACCTGCAGGC AGAGTGGCGA CCTCACTTAC 
GACTGTGCCT GTCTTCCTGG GTTTGAGGGT CAGAATTGTG AAGTGAACGT GGACGACTGT 
CCAGGACACC GATGTCTCAA TGGGGGGACA TGCGTGGATG GCGTCAACAC CTATAACTGC 
CAGTGCCCTC CTGAGTGGAC AGGCCAGTTC TGCACGGAGG ACGTGGATGA GTGTCAGCTG 
CAGCCCAACG CCTGCCACAA TGGGGGTACC TGCTTCAACA CGCTGGGTGG CCACAGCTGC 
GTGTGTGTCA ATGGCTGGAC AGGCGAGAGC TGCAGTCAGA ATATCGATGA CTGTGCCACA 
GCCGTGTGCT TCCATGGGGC CACCTGCCAT GACCGCGTGG CTTCTTTCTA CTGTGCCTGC 
CCCATGGGCA AGACTGGCCT CCTGTGTCAC CTGGATGACG CCTGTGTCAG CAACCCCTGC 
CACGAGGATG CTATCTGTGA CACAAATCCG GTGAACGGCC GGGCCATTTG CACCTGTCCT 
CCCGGCTTCA CGGGTGGGGC ATGTGACCAG GATGTGGACG AGTGCTCTAT CGGCGCCAAC 
CCCTGCGAGC ACTTGGGCAG GTGCGTGAAC ACGCAGGGCT CCTTCCTGTG CCAGTGCGGT 
CGTGGCTACA CTGGACCTCG CTGTGAGACC GATGTCAACG AGTGTCTGTC GGGGCCCTGC 
CGAAACCAGG CCACGTGCCT CGACCGCATA GGCCAGTTCA CCTGTATCTG TATGGCAGGC 
TTCACAGGAA CCTATTGCGA GGTGGACATT GACGAGTGTC AGAGTAGCCC CTGTGTCAAC 
GGTGGGGTCT GCAAGGACCG AGTCAATGGC TTCAGCTGCA CCTGCCCCTC GGGCTTCAGC 
GGCTCCACGT GTCAGCTGGA CGTGGACGAA TGCGCCAGCA CGCCCTGCAG GAATGGCGCC 
AAATGCGTGG ACCAGCCCGA TGGCTACGAG TGCCGCTGTG CCGAGGGCTT TGAGGGCACG 
CTGTGTGATC GCAACGTGGA CGACTGCTCC CCTGACCCAT GCCACCATGG TCGCTGCGTG 
GATGGCATCG CCAGCTTCTC ATGTGCCTGT GCTCCTGGCT ACACGGGCAC ACGCTGCGAG 
AGCCAGGTGG ACGAATGCCG CAGCCAGCCC TGCCGCCATG GCGGCAAATG CCTAGACCTG 
GTGGACAAGT ACCTCTGCCG CTGCCCTTCT GGGACCACAG GTGTGAACTG CGAAGTGAAC 
ATTGACGACT GTGCCAGCAA CCCCTGCACC TTTGGAGTCT GCCGTGATGG CATCAACCGC 
TACGACTGTG TCTGCCAACC TGGCTTCACA GGGCCCCTTT GTAACGTGGA GATCAATGAG 
TGTGCTTCCA GCCCATGCGG CGAGGGAGGT TCCTGTGTGG ATGGGGAAAA TGGCTTCCGC 
TGCCTCTGCC CGCCTGGCTC CTTGCCCCCA CTCTGCCTCC CCCCGAGCCA TCCCTGTGCC 
CATGAGCCCT GCAGTCACGG CATCTGCTAT GATGCACCTG GCGGGTTCCG CTGTGTGTGT 
GAGCCTGGCT GGAGTGGCCC CCGCTGCAGC CAGAGCCTGG CCCGAGACGC CTGTGAGTCC 
CAGCCGTGCA GGGCCGGTGG GACATGCAGC AGCGATGGAA TGGGTTTCCA CTGCACCTGC 
CCGCCTGGTG TCCAGGGACG TCAGTGTGAA CTCCTCTCCC CCTGCACCCC GAACCCCTGT 
GAGCATGGGG GCCGCTGCGA GTCTGCCCCT GGCCAGCTGC CTGTCTGCTC CTGCCCCCAG 
GGCTGGCAAG ACCCATGCCT GAACGGTGGC TCGTGCCAAG ACGGCGTGGG CTCCTTTTCC 
TGCTCCTGCC TCCCTGGTTT CGCCGGCCCA CGATGCGCCC GCGATGTGGA TGAGTGCCTG 
AGCAACCCCT GCGGCCCGGG CACCTGTACC GACCACGTGG CCTCCTTCAC CTGCACCTGC 
CCGCCAGGCT ACGGAGGCTT CCACTGCGAA CAGGACCTGC CCGACTGCAG CCCCAGCTCC 
TGCTTCAATG GCGGGACCTG TGTGGACGGC GTGAACTCGT TCAGCTGCCT GTGCCGTCCC 
GGCTACACAG GAGCCCACTG CCAACATGAG GCAGACCCCT GCCTCTCGCG GCCCTGCCTA 
CACGGGGGCG TCTGCAGCGC CGCCCACCCT GGCTTCCGCT GCACCTGCCT CGAGAGCTTC 
ACGGGCCCGC AGTGCCAGAC GCTGGTGGAT TGGTGCAGCC GCCAGCCTTG TCAAAACGGG 
GGTCGCTGCG TCCAGACTGG GGCCTATTGC CTTTGTCCCC CTGGATGGAG CGGACGCCTC 
TGTGACATCC GAAGCTTGCC CTGCAGGGAG GCCGCAGCCC AGATCGGGGT GCGGCTGGAG 
CAGCTGTGTC AGGCGGGTGG GCAGTGTGTG GATGAAGACA GCTCCCACTA CTGCGTGTGC 
CCAGAGGGCC GTACTGGTAG CCACTGTGAG CAGGAGGTGG ACCCCTGCTT GGCCCAGCCC 
TGCCAGCATG GGGGGACCTG CCGTGGCTAT ATGGGGGGCT ACATGTGTGA GTGTCTTCCT 
GGCTACAATG GTGATAACTG TGAGGACGAC GTGGACGAGT GTGCCTCCCA GCCCTGCCAG 
CACGGGGGTT CATGCATTGA CCTCGTGGCC CGCTATCTCT GCTCCTGTCC CCCAGGAACG 
CTGGGGGTGC TCTGCGAGAT TAATGAGGAT GACTGCGGCC CAGGCCCACC GCTGGACTCA 
GGGCCCCGGT GCCTACACAA TGGCACCTGC GTGGACCTGG TGGGTGGTTT CCGCTGCACC 
TGTCCCCCAG GATACACTGG TTTGCGCTGC GAGGCAGACA TCAATGAGTG TCGCTCAGGT 
GCCTGCCACG CGGCACACAC CCGGGACTGC CTGCAGGACC CAGGCGGAGG TTTCCGTTGC 
CTTTGTCATG CTGGCTTCTC AGGTCCTCGC TGTCAGACTG TCCTGTCTCC CTGCGAGTCC 
CAGCCATGCC AGCATGGAGG CCAGTGCCGT CCTAGCCCGG GTCCTGGGGG TGGGCTGACC 
TTCACCTGTC ACTGTGCCCA GCCGTTCTGG GGTCCGCGTT GCGAGCGGGT GGCGCGCTCC 
TGCCGGGAGC TGCAGTGCCC GGTGGGCGTC CCATGCCAGC AGACGCCCCG CGGGCCGCGC 
TGCGCCTGCC CCCCAGGGTT GTCGGGACCC TCCTGCCGCA GCTTCCCGGG GTCGCCGCCG 
GGGGCCAGCA ACGCCAGCTG CGCGGCCGCC CCCTGTCTCC ACGGGGGCTC CTGCCGCCCC 
GCGCCGCTCG CGCCCTTCTT CCGCTGCGCT TGCGCGCAGG GCTGGACCGG GCCGCGCTGC 
GAGGCGCCCG CCGCGGCACC CGAGGTCTCG GAGGAGCCGC GGTGCCCGCG CGCCGCCTGC 
CAGGCCAAGC GCGGGGACCA GCGCTGCGAC CGCGAGTGCA ACAGCCCAGG CTGCGGCTGG 
GACGGCGGCG ACTGCTCGCT GAGCGTGGGC GACCCCTGGC GGCAATGCGA GGCGCTGCAG 
TGCTGGCGCC TCTTCAACAA CAGCCGCTGC GACCCCGCCT GCAGCTCGCC CGCCTGCCTC 
TACGACAACT TCGACTGCCA CGCCGGTGGC CGCGAGCGCA CTTGCAACCC GGTGTACGAG 
AAGTACTGCG CCGACCACTT TGCCGACGGC CGCTGCGACC AGGGCTGCAA CACGGAGGAG 
TGCGGCTGGG ATGGGCTGGA TTGTGCCAGC GAGGTGCCGG CCCTGCTGGC CCGCGGCGTG 
CTGGTGCTCA CAGTGCTGCT GCCGCCAGAG GAGCTACTGC GTTCCAGCGC CGACTTTCTG 
CAGCGGCTCA GCGCCATCCT GCGCACCTCG CTGCGCTTCC GCCTGGACGC GCACGGCCAG 
GCCATGGTCT TCCCTTACCA CCGGCCTAGT CCTGGCTCCG AACCCCGGGC CCGTCGGGAG 
CTGGCCCCCG AGGTGATCGG CTCGGTAGTA ATGCTGGAGA TTGACAACCG GCTCTGCCTG 
CAGTCGCCTG AGAATGATCA CTGCTTCCCC GATGCCCAGA GCGCCGCTGA CTACCTGGGA 
GCGTTGTCAG CGGTGGAGCG CCTGGACTTC CCGTACCCAC TGCGGGACGT GCGGGGGGAG 
CCGCTGGAGC CTCCAGAACC CAGCGTCCCG CTGCTGCCAC TGCTAGTGGC GGGCGCTGTC 
TTGCTGCTGG TCATTCTCGT CCTGGGTGTC ATGGTGGCCC GGCGCAAGCG CGAGCACAGC 
ACCCTCTGGT TCCCTGAGGG CTTCTCACTG CACAAGGACG TGGCCTCTGG TCACAAGGGC 
CGGCGGGAAC CCGTGGGCCA GGACGCGCTG GGCATGAAGA ACATGGCCAA GGGTGAGAGC 
CTGATGGGGG AGGTGGCCAC AGACTGGATG GACACAGAGT GCCCAGAGGC CAAGCGGCTA 
AAGGTAGAGG AGCCAGGCAT GGGGGCTGAG GAGGCTGTGG ATTGCCGTCA GTGGACTCAA 
CACCATCTGG TTGCTGCTGA CATCCGCGTG GCACCAGCCA TGGCACTGAC ACCACCACAG 
GGCGACGCAG ATGCTGATGG CATGGATGTC AATGTGCGTG GCCCAGATGG CTTCACCCCG 
CTAATGCTGG CTTCCTTCTG TGGGGGGGCT CTGGAGCCAA TGCCAACTGA AGAGGATGAG 
GCAGATGACA CATCAGCTAG CATCATCTCC GACCTGATCT GCCAGGGGGC TCAGCTTGGG 
GCACGGACTG ACCGTACTGG CGAGACTGCT TTGCACCTGG CTGCCCGTTA TGCCCGTGCT 
GATGCAGCCA AGCGGCTGCT GGATGCTGGG GCAGACACCA ATGCCCAGGA CCACTCAGGC 
CGCACTCCCC TGCACACAGC TGTCACAGCC GATGCCCAGG GTGTCTTCCA GATTCTCATC 
CGAAACCGCT CTACAGACTT GGATGCCCGC ATGGCAGATG GCTCAACGGC ACTGATCCTG 
GCGGCCCGCC TGGCAGTAGA GGGCATGGTG GAAGAGCTCA TCGCCAGCCA TGCTGATGTC 
AATGCTGTGG ATGAGCTTGG GAAATCAGCC TTACACTGGG CTGCGGCTGT GAACAACGTG 
GAAGCCACTT TGGCCCTGCT CAAAAATGGA GCCAATAAGG ACATGCAGGA TAGCAAGGAG 
GAGACCCCCC TATTCCTGGC CGCCCGCGAG GGCAGCTATG AGGCTGCCAA GCTGCTGTTG 
GACCACTTTG CCAACCGTGA GATCACCGAC CACCTGGACA GGCTGCCGCG GGACGTAGCC 
CAGGAGAGAC TGCACCAGGA CATCGTGCGC TTGCTGGATC AACCCAGTGG GCCCCGCAGC 
CCCCCCGGTC CCCACGGCCT GGGGCCTCTG CTCTGTCCTC CAGGGGCCTT CCTCCCTGGC 
CTCAAAGCGG CACAGTCGGG GTCCAAGAAG AGCAGGAGGC CCCCCGGGAA GGCGGGGCTG 
GGGCCGCAGG GGCCCCGGGG GCGGGGCAAG AAGCTGACGC TGGCCTGCCC GGGCCCCCTG 
GCTGACAGCT CGGTCACGCT GTCGCCCGTG GACTCGCTGG ACTCCCCGCG GCCTTTCGGT 
GGGCCCCCTG CTTCCCCTGG TGGCTTCCCC CTTGAGGGGC CCTATGCAGC TGCCACTGCC 
ACTGCAGTGT CTCTGGCACA GCTTGGTGGC CCAGGCCGGG CGGGTCTAGG GCGCCAGCCC 
CCTGGAGGAT GTGTACTCAG CCTGGGCCTG CTGAACCCTG TGGCTGTGCC CCTCGATTGG 
GCCCGGCTGC CCCCACCTGC CCCTCCAGGC CCCTCGTTCC TGCTGCCACT GGCGCCGGGA 
CCCCAGCTGC TCAACCCAGG GACCCCCGTC TCCCCGCAGG AGCGGCCCCC GCCTTACCTG 
GCAGTCCCAG GACATGGCGA GGAGTACCCG GCGGCTGGGG CACACAGCAG CCCCCCAAAG 
GCCCGCTTCC TGCGGGTTCC CAGTGAGCAC CCTTACCTGA CCCCATCCCC CGAATCCCCT 
GAGCACTGGG CCAGCCCCTC ACCTCCCTCC CTCTCAGACT GGTCCGAATC CACGCCTAGC 
CCAGCCACTG CCACTGGGGC CATGGCCACC ACCACTGGGG CACTGCCTGC CCAGCCACTT 
CCCTTGTCTG TTCCCAGCTC CCTTGCTCAG GCCCAGACCC AGCTGGGGCC CCAGCCGGAA 
GTTACCCCCA AGAGGCAAGT GTTGGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005259981.1
CDS16..6825
Translation

Target ORF information:

RefSeq Version XM_005259924.4
Organism Homo sapiens(human)
Definition Homo sapiens notch receptor 3 (NOTCH3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005259924.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
5041
5101
5161
5221
5281
5341
5401
5461
5521
5581
5641
5701
5761
5821
5881
5941
6001
6061
6121
6181
6241
6301
6361
6421
6481
6541
6601
6661
6721
6781
ATGGGGCCGG GGGCCCGTGG CCGCCGCCGC CGCCGTCGCC CGATGTCGCC GCCACCGCCA 
CCGCCACCCG TGCGGGCGCT GCCCCTGCTG CTGCTGCTAG CGGGGCCGGG GGCTGCAGCC 
CCCCCTTGCC TGGACGGAAG CCCGTGTGCA AATGGAGGTC GTTGCACCCA GCTGCCCTCC 
CGGGAGGCTG CCTGCCTGTG CCCGCCTGGC TGGGTGGGTG AGCGGTGTCA GCTGGAGGAC 
CCCTGTCACT CAGGCCCCTG TGCTGGCCGT GGTGTCTGCC AGAGTTCAGT GGTGGCTGGC 
ACCGCCCGAT TCTCATGCCG GTGCCCCCGT GGCTTCCGAG GCCCTGACTG CTCCCTGCCA 
GATCCCTGCC TCAGCAGCCC TTGTGCCCAC GGTGCCCGCT GCTCAGTGGG GCCCGATGGA 
CGCTTCCTCT GCTCCTGCCC ACCTGGCTAC CAGGGCCGCA GCTGCCGAAG CGACGTGGAT 
GAGTGCCGGG TGGGTGAGCC CTGCCGCCAT GGTGGCACCT GCCTCAACAC ACCTGGCTCC 
TTCCGCTGCC AGTGTCCAGC TGGCTACACA GGGCCACTAT GTGAGAACCC CGCGGTGCCC 
TGTGCACCCT CACCATGCCG TAACGGGGGC ACCTGCAGGC AGAGTGGCGA CCTCACTTAC 
GACTGTGCCT GTCTTCCTGG GTTTGAGGGT CAGAATTGTG AAGTGAACGT GGACGACTGT 
CCAGGACACC GATGTCTCAA TGGGGGGACA TGCGTGGATG GCGTCAACAC CTATAACTGC 
CAGTGCCCTC CTGAGTGGAC AGGCCAGTTC TGCACGGAGG ACGTGGATGA GTGTCAGCTG 
CAGCCCAACG CCTGCCACAA TGGGGGTACC TGCTTCAACA CGCTGGGTGG CCACAGCTGC 
GTGTGTGTCA ATGGCTGGAC AGGCGAGAGC TGCAGTCAGA ATATCGATGA CTGTGCCACA 
GCCGTGTGCT TCCATGGGGC CACCTGCCAT GACCGCGTGG CTTCTTTCTA CTGTGCCTGC 
CCCATGGGCA AGACTGGCCT CCTGTGTCAC CTGGATGACG CCTGTGTCAG CAACCCCTGC 
CACGAGGATG CTATCTGTGA CACAAATCCG GTGAACGGCC GGGCCATTTG CACCTGTCCT 
CCCGGCTTCA CGGGTGGGGC ATGTGACCAG GATGTGGACG AGTGCTCTAT CGGCGCCAAC 
CCCTGCGAGC ACTTGGGCAG GTGCGTGAAC ACGCAGGGCT CCTTCCTGTG CCAGTGCGGT 
CGTGGCTACA CTGGACCTCG CTGTGAGACC GATGTCAACG AGTGTCTGTC GGGGCCCTGC 
CGAAACCAGG CCACGTGCCT CGACCGCATA GGCCAGTTCA CCTGTATCTG TATGGCAGGC 
TTCACAGGAA CCTATTGCGA GGTGGACATT GACGAGTGTC AGAGTAGCCC CTGTGTCAAC 
GGTGGGGTCT GCAAGGACCG AGTCAATGGC TTCAGCTGCA CCTGCCCCTC GGGCTTCAGC 
GGCTCCACGT GTCAGCTGGA CGTGGACGAA TGCGCCAGCA CGCCCTGCAG GAATGGCGCC 
AAATGCGTGG ACCAGCCCGA TGGCTACGAG TGCCGCTGTG CCGAGGGCTT TGAGGGCACG 
CTGTGTGATC GCAACGTGGA CGACTGCTCC CCTGACCCAT GCCACCATGG TCGCTGCGTG 
GATGGCATCG CCAGCTTCTC ATGTGCCTGT GCTCCTGGCT ACACGGGCAC ACGCTGCGAG 
AGCCAGGTGG ACGAATGCCG CAGCCAGCCC TGCCGCCATG GCGGCAAATG CCTAGACCTG 
GTGGACAAGT ACCTCTGCCG CTGCCCTTCT GGGACCACAG GTGTGAACTG CGAAGTGAAC 
ATTGACGACT GTGCCAGCAA CCCCTGCACC TTTGGAGTCT GCCGTGATGG CATCAACCGC 
TACGACTGTG TCTGCCAACC TGGCTTCACA GGGCCCCTTT GTAACGTGGA GATCAATGAG 
TGTGCTTCCA GCCCATGCGG CGAGGGAGGT TCCTGTGTGG ATGGGGAAAA TGGCTTCCGC 
TGCCTCTGCC CGCCTGGCTC CTTGCCCCCA CTCTGCCTCC CCCCGAGCCA TCCCTGTGCC 
CATGAGCCCT GCAGTCACGG CATCTGCTAT GATGCACCTG GCGGGTTCCG CTGTGTGTGT 
GAGCCTGGCT GGAGTGGCCC CCGCTGCAGC CAGAGCCTGG CCCGAGACGC CTGTGAGTCC 
CAGCCGTGCA GGGCCGGTGG GACATGCAGC AGCGATGGAA TGGGTTTCCA CTGCACCTGC 
CCGCCTGGTG TCCAGGGACG TCAGTGTGAA CTCCTCTCCC CCTGCACCCC GAACCCCTGT 
GAGCATGGGG GCCGCTGCGA GTCTGCCCCT GGCCAGCTGC CTGTCTGCTC CTGCCCCCAG 
GGCTGGCAAG ACCCATGCCT GAACGGTGGC TCGTGCCAAG ACGGCGTGGG CTCCTTTTCC 
TGCTCCTGCC TCCCTGGTTT CGCCGGCCCA CGATGCGCCC GCGATGTGGA TGAGTGCCTG 
AGCAACCCCT GCGGCCCGGG CACCTGTACC GACCACGTGG CCTCCTTCAC CTGCACCTGC 
CCGCCAGGCT ACGGAGGCTT CCACTGCGAA CAGGACCTGC CCGACTGCAG CCCCAGCTCC 
TGCTTCAATG GCGGGACCTG TGTGGACGGC GTGAACTCGT TCAGCTGCCT GTGCCGTCCC 
GGCTACACAG GAGCCCACTG CCAACATGAG GCAGACCCCT GCCTCTCGCG GCCCTGCCTA 
CACGGGGGCG TCTGCAGCGC CGCCCACCCT GGCTTCCGCT GCACCTGCCT CGAGAGCTTC 
ACGGGCCCGC AGTGCCAGAC GCTGGTGGAT TGGTGCAGCC GCCAGCCTTG TCAAAACGGG 
GGTCGCTGCG TCCAGACTGG GGCCTATTGC CTTTGTCCCC CTGGATGGAG CGGACGCCTC 
TGTGACATCC GAAGCTTGCC CTGCAGGGAG GCCGCAGCCC AGATCGGGGT GCGGCTGGAG 
CAGCTGTGTC AGGCGGGTGG GCAGTGTGTG GATGAAGACA GCTCCCACTA CTGCGTGTGC 
CCAGAGGGCC GTACTGGTAG CCACTGTGAG CAGGAGGTGG ACCCCTGCTT GGCCCAGCCC 
TGCCAGCATG GGGGGACCTG CCGTGGCTAT ATGGGGGGCT ACATGTGTGA GTGTCTTCCT 
GGCTACAATG GTGATAACTG TGAGGACGAC GTGGACGAGT GTGCCTCCCA GCCCTGCCAG 
CACGGGGGTT CATGCATTGA CCTCGTGGCC CGCTATCTCT GCTCCTGTCC CCCAGGAACG 
CTGGGGGTGC TCTGCGAGAT TAATGAGGAT GACTGCGGCC CAGGCCCACC GCTGGACTCA 
GGGCCCCGGT GCCTACACAA TGGCACCTGC GTGGACCTGG TGGGTGGTTT CCGCTGCACC 
TGTCCCCCAG GATACACTGG TTTGCGCTGC GAGGCAGACA TCAATGAGTG TCGCTCAGGT 
GCCTGCCACG CGGCACACAC CCGGGACTGC CTGCAGGACC CAGGCGGAGG TTTCCGTTGC 
CTTTGTCATG CTGGCTTCTC AGGTCCTCGC TGTCAGACTG TCCTGTCTCC CTGCGAGTCC 
CAGCCATGCC AGCATGGAGG CCAGTGCCGT CCTAGCCCGG GTCCTGGGGG TGGGCTGACC 
TTCACCTGTC ACTGTGCCCA GCCGTTCTGG GGTCCGCGTT GCGAGCGGGT GGCGCGCTCC 
TGCCGGGAGC TGCAGTGCCC GGTGGGCGTC CCATGCCAGC AGACGCCCCG CGGGCCGCGC 
TGCGCCTGCC CCCCAGGGTT GTCGGGACCC TCCTGCCGCA GCTTCCCGGG GTCGCCGCCG 
GGGGCCAGCA ACGCCAGCTG CGCGGCCGCC CCCTGTCTCC ACGGGGGCTC CTGCCGCCCC 
GCGCCGCTCG CGCCCTTCTT CCGCTGCGCT TGCGCGCAGG GCTGGACCGG GCCGCGCTGC 
GAGGCGCCCG CCGCGGCACC CGAGGTCTCG GAGGAGCCGC GGTGCCCGCG CGCCGCCTGC 
CAGGCCAAGC GCGGGGACCA GCGCTGCGAC CGCGAGTGCA ACAGCCCAGG CTGCGGCTGG 
GACGGCGGCG ACTGCTCGCT GAGCGTGGGC GACCCCTGGC GGCAATGCGA GGCGCTGCAG 
TGCTGGCGCC TCTTCAACAA CAGCCGCTGC GACCCCGCCT GCAGCTCGCC CGCCTGCCTC 
TACGACAACT TCGACTGCCA CGCCGGTGGC CGCGAGCGCA CTTGCAACCC GGTGTACGAG 
AAGTACTGCG CCGACCACTT TGCCGACGGC CGCTGCGACC AGGGCTGCAA CACGGAGGAG 
TGCGGCTGGG ATGGGCTGGA TTGTGCCAGC GAGGTGCCGG CCCTGCTGGC CCGCGGCGTG 
CTGGTGCTCA CAGTGCTGCT GCCGCCAGAG GAGCTACTGC GTTCCAGCGC CGACTTTCTG 
CAGCGGCTCA GCGCCATCCT GCGCACCTCG CTGCGCTTCC GCCTGGACGC GCACGGCCAG 
GCCATGGTCT TCCCTTACCA CCGGCCTAGT CCTGGCTCCG AACCCCGGGC CCGTCGGGAG 
CTGGCCCCCG AGGTGATCGG CTCGGTAGTA ATGCTGGAGA TTGACAACCG GCTCTGCCTG 
CAGTCGCCTG AGAATGATCA CTGCTTCCCC GATGCCCAGA GCGCCGCTGA CTACCTGGGA 
GCGTTGTCAG CGGTGGAGCG CCTGGACTTC CCGTACCCAC TGCGGGACGT GCGGGGGGAG 
CCGCTGGAGC CTCCAGAACC CAGCGTCCCG CTGCTGCCAC TGCTAGTGGC GGGCGCTGTC 
TTGCTGCTGG TCATTCTCGT CCTGGGTGTC ATGGTGGCCC GGCGCAAGCG CGAGCACAGC 
ACCCTCTGGT TCCCTGAGGG CTTCTCACTG CACAAGGACG TGGCCTCTGG TCACAAGGGC 
CGGCGGGAAC CCGTGGGCCA GGACGCGCTG GGCATGAAGA ACATGGCCAA GGGTGAGAGC 
CTGATGGGGG AGGTGGCCAC AGACTGGATG GACACAGAGT GCCCAGAGGC CAAGCGGCTA 
AAGGTAGAGG AGCCAGGCAT GGGGGCTGAG GAGGCTGTGG ATTGCCGTCA GTGGACTCAA 
CACCATCTGG TTGCTGCTGA CATCCGCGTG GCACCAGCCA TGGCACTGAC ACCACCACAG 
GGCGACGCAG ATGCTGATGG CATGGATGTC AATGTGCGTG GCCCAGATGG CTTCACCCCG 
CTAATGCTGG CTTCCTTCTG TGGGGGGGCT CTGGAGCCAA TGCCAACTGA AGAGGATGAG 
GCAGATGACA CATCAGCTAG CATCATCTCC GACCTGATCT GCCAGGGGGC TCAGCTTGGG 
GCACGGACTG ACCGTACTGG CGAGACTGCT TTGCACCTGG CTGCCCGTTA TGCCCGTGCT 
GATGCAGCCA AGCGGCTGCT GGATGCTGGG GCAGACACCA ATGCCCAGGA CCACTCAGGC 
CGCACTCCCC TGCACACAGC TGTCACAGCC GATGCCCAGG GTGTCTTCCA GATTCTCATC 
CGAAACCGCT CTACAGACTT GGATGCCCGC ATGGCAGATG GCTCAACGGC ACTGATCCTG 
GCGGCCCGCC TGGCAGTAGA GGGCATGGTG GAAGAGCTCA TCGCCAGCCA TGCTGATGTC 
AATGCTGTGG ATGAGCTTGG GAAATCAGCC TTACACTGGG CTGCGGCTGT GAACAACGTG 
GAAGCCACTT TGGCCCTGCT CAAAAATGGA GCCAATAAGG ACATGCAGGA TAGCAAGGAG 
GAGACCCCCC TATTCCTGGC CGCCCGCGAG GGCAGCTATG AGGCTGCCAA GCTGCTGTTG 
GACCACTTTG CCAACCGTGA GATCACCGAC CACCTGGACA GGCTGCCGCG GGACGTAGCC 
CAGGAGAGAC TGCACCAGGA CATCGTGCGC TTGCTGGATC AACCCAGTGG GCCCCGCAGC 
CCCCCCGGTC CCCACGGCCT GGGGCCTCTG CTCTGTCCTC CAGGGGCCTT CCTCCCTGGC 
CTCAAAGCGG CACAGTCGGG GTCCAAGAAG AGCAGGAGGC CCCCCGGGAA GGCGGGGCTG 
GGGCCGCAGG GGCCCCGGGG GCGGGGCAAG AAGCTGACGC TGGCCTGCCC GGGCCCCCTG 
GCTGACAGCT CGGTCACGCT GTCGCCCGTG GACTCGCTGG ACTCCCCGCG GCCTTTCGGT 
GGGCCCCCTG CTTCCCCTGG TGGCTTCCCC CTTGAGGGGC CCTATGCAGC TGCCACTGCC 
ACTGCAGTGT CTCTGGCACA GCTTGGTGGC CCAGGCCGGG CGGGTCTAGG GCGCCAGCCC 
CCTGGAGGAT GTGTACTCAG CCTGGGCCTG CTGAACCCTG TGGCTGTGCC CCTCGATTGG 
GCCCGGCTGC CCCCACCTGC CCCTCCAGGC CCCTCGTTCC TGCTGCCACT GGCGCCGGGA 
CCCCAGCTGC TCAACCCAGG GACCCCCGTC TCCCCGCAGG AGCGGCCCCC GCCTTACCTG 
GCAGTCCCAG GACATGGCGA GGAGTACCCG GCGGCTGGGG CACACAGCAG CCCCCCAAAG 
GCCCGCTTCC TGCGGGTTCC CAGTGAGCAC CCTTACCTGA CCCCATCCCC CGAATCCCCT 
GAGCACTGGG CCAGCCCCTC ACCTCCCTCC CTCTCAGACT GGTCCGAATC CACGCCTAGC 
CCAGCCACTG CCACTGGGGC CATGGCCACC ACCACTGGGG CACTGCCTGC CCAGCCACTT 
CCCTTGTCTG TTCCCAGCTC CCTTGCTCAG GCCCAGACCC AGCTGGGGCC CCAGCCGGAA 
GTTACCCCCA AGAGGCAAGT GTTGGCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

The common oncogenomic program of NOTCH1 and NOTCH3 signaling in T-cell acute lymphoblastic leukemia.
PloS one12(10)e0185762(2017)
Choi SH,Severson E,Pear WS,Liu XS,Aster JC,Blacklow SC