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NPC1 cDNA ORF clone, Homo sapiens (human)

Gene Symbol NPC1
Entrez Gene ID 4864
Full Name Niemann-Pick disease, type C1
Synonyms NPC
General protein information
Preferred Names
Niemann-Pick C1 protein
Names
Niemann-Pick C1 protein
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

18

18q11.2

Summary This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.[provided by RefSeq, Aug 2009]. lac of sum
Disorder MIM:

607623

Disorder Html: Niemann-Pick disease, type C1, 257220 (3); Niemann-Pick disease,

mRNA and Protein(s)

mRNA Protein Name
XM_005258277 XP_005258334 Niemann-Pick C1 protein isoform X1
XM_005258278 XP_005258335 Niemann-Pick C1 protein isoform X2
XM_005258279 XP_005258336 Niemann-Pick C1 protein isoform X3
XM_006722479 XP_006722542 Niemann-Pick C1 protein isoform X4
XM_011526015 XP_011524317 Niemann-Pick C1 protein isoform X5
NM_000271 NP_000262 Niemann-Pick C1 protein precursor



Homo sapiens (human) NPC1 NP_000262.2
Pan troglodytes (chimpanzee) NPC1 XP_001155285.1
Macaca mulatta (Rhesus monkey) NPC1 XP_002800934.1
Canis lupus familiaris (dog) NPC1 NP_001003107.1
Bos taurus (cattle) NPC1 NP_777183.1
Mus musculus (house mouse) Npc1 NP_032746.2
Rattus norvegicus (Norway rat) Npc1 NP_705888.2
Gallus gallus (chicken) NPC1 XP_419162.3
Danio rerio (zebrafish) npc1 NP_001230804.1
Drosophila melanogaster (fruit fly) Npc1a NP_001188769.1
Arabidopsis thaliana (thale cress) AT4G38350 NP_001190953.1
Arabidopsis thaliana (thale cress) AT1G42470 NP_174975.2
Xenopus (Silurana) tropicalis (western clawed frog) npc1 XP_004915269.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NPC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu51216 XM_005258277 PREDICTED: Homo sapiens Niemann-Pick disease, type C1 (NPC1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu51217 XM_005258278 PREDICTED: Homo sapiens Niemann-Pick disease, type C1 (NPC1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu51218 XM_005258279 PREDICTED: Homo sapiens Niemann-Pick disease, type C1 (NPC1), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu51219 XM_006722479 PREDICTED: Homo sapiens Niemann-Pick disease, type C1 (NPC1), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $643.30
$919.00
OHu59314 XM_011526015 PREDICTED: Homo sapiens Niemann-Pick disease, type C1 (NPC1), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $559.30
$799.00
OHu27327 NM_000271 Homo sapiens Niemann-Pick disease, type C1 (NPC1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 20 $384.30
$549.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu51216
Clone ID Related Accession (Same CDS sequence) XM_005258277
Accession Version XM_005258277.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Niemann-Pick C1 protein isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010966.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
CTCCCTGTCT TACTCAGTTA CATAGCAAAG CTTCTGGAAA GAACTATCTA CTTGTGCATC
TCACAGCACA TTCATTATGC AACCCAGATG GCTCCACTAC AGTGTCTCAT GACCGCCTCA
GACAGATGTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005258334.1
CDS164..4075
Misc Feature(1)236..3967(+)
Misc Feature(2)2159..2620(+)
Translation

Target ORF information:

RefSeq Version XM_005258277
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Niemann-Pick disease, type C1 (NPC1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005258277

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
CTCCCTGTCT TACTCAGTTA CATAGCAAAG CTTCTGGAAA GAACTATCTA CTTGTGCATC
TCACAGCACA TTCATTATGC AACCCAGATG GCTCCACTAC AGTGTCTCAT GACCGCCTCA
GACAGATGTT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51217
Clone ID Related Accession (Same CDS sequence) XM_005258278
Accession Version XM_005258278.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Niemann-Pick C1 protein isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010966.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578832532. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
CTCCCTGTCT TACTCAGTTA CATAGGGCCA TCAGTAAATA AAGCCAAAAG TTGTGCCACT
GAAGAGCGAT ACAAAGGAAC AGAGCGCGAA CGGCTTCTAA ATTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005258335.1
CDS164..4051
Misc Feature(1)236..3970(+)
Misc Feature(2)2159..2620(+)
Translation

Target ORF information:

RefSeq Version XM_005258278
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Niemann-Pick disease, type C1 (NPC1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005258278

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
CTCCCTGTCT TACTCAGTTA CATAGGGCCA TCAGTAAATA AAGCCAAAAG TTGTGCCACT
GAAGAGCGAT ACAAAGGAAC AGAGCGCGAA CGGCTTCTAA ATTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51218
Clone ID Related Accession (Same CDS sequence) XM_005258279
Accession Version XM_005258279.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Niemann-Pick C1 protein isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010966.15) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGCAAAGC TTCTGGAAAG AACTATCTAC
TTGTGCATCT CACAGCACAT TCATTATGCA ACCCAGATGG CTCCACTACA GTGTCTCATG
ACCGCCTCAG ACAGATGTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005258336.1
CDS164..4024
Misc Feature(1)236..3916(+)
Misc Feature(2)2108..2569(+)
Translation

Target ORF information:

RefSeq Version XM_005258279
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Niemann-Pick disease, type C1 (NPC1), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005258279

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGCAAAGC TTCTGGAAAG AACTATCTAC
TTGTGCATCT CACAGCACAT TCATTATGCA ACCCAGATGG CTCCACTACA GTGTCTCATG
ACCGCCTCAG ACAGATGTTA A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu51219
Clone ID Related Accession (Same CDS sequence) XM_006722479
Accession Version XM_006722479.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3822bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Niemann-Pick C1 protein isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010966.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578832533. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
CTCCCTGTCT TACTCAGTTA CATAGGCCCA TTCAATTCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006722542.1
CDS164..3985
Misc Feature(1)236..3970(+)
Misc Feature(2)2159..2620(+)
Translation

Target ORF information:

RefSeq Version XM_006722479
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Niemann-Pick disease, type C1 (NPC1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006722479

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAACG
GCTTGGCTCT TAACCTCCAC CTTCCCTTCC TCTCCCGTTC TTCCAGGAGA GGCGTCCTGC
TGTGACCCTG TCAGCGCAGC ATTTGAGGGC TGCTTGAGGC GGCTGTTCAC ACGCTGGGGG
TCTTTCTGCG TCCGAAACCC TGGCTGTGTC ATTTTCTTCT CGCTGGTCTT CATTACTGCG
TGTTCGTCAG GCCTGGTGTT TGTCCGGGTC ACAACCAATC CAGTTGACCT CTGGTCAGCC
CCCAGCAGCC AGGCTCGCCT GGAAAAAGAG TACTTTGACC AGCACTTTGG GCCTTTCTTC
CGGACGGAGC AGCTCATCAT CCGGGCCCCT CTCACTGACA AACACATTTA CCAGCCATAC
CCTTCGGGAG CTGATGTACC CTTTGGACCT CCGCTTGACA TACAGATACT GCACCAGGTT
CTTGACTTAC AAATAGCCAT CGAAAACATT ACTGCCTCTT ATGACAATGA GACTGTGACA
CTTCAAGACA TCTGCTTGGC CCCTCTTTCA CCGTATAACA CGAACTGCAC CATTTTGAGT
GTGTTAAATT ACTTCCAGAA CAGCCATTCC GTGCTGGACC ACAAGAAAGG GGACGACTTC
TTTGTGTATG CCGATTACCA CACGCACTTT CTGTACTGCG TACGGGCTCC TGCCTCTCTG
AATGATACAA GTTTGCTCCA TGACCCTTGT CTGGGTACGT TTGGTGGACC AGTGTTCCCG
TGGCTTGTGT TGGGAGGCTA TGATGATCAA AACTACAATA ACGCCACTGC CCTTGTGATT
ACCTTCCCTG TCAATAATTA CTATAATGAT ACAGAGAAGC TCCAGAGGGC CCAGGCCTGG
GAAAAAGAGT TTATTAATTT TGTGAAAAAC TACAAGAATC CCAATCTGAC CATTTCCTTC
ACTGCTGAAC GAAGTATTGA AGATGAACTA AATCGTGAAA GTGACAGTGA TGTCTTCACC
GTTGTAATTA GCTATGCCAT CATGTTTCTA TATATTTCCC TAGCCTTGGG GCACATGAAA
AGCTGTCGCA GGCTTCTGGT GGATTCGAAG GTCTCACTAG GCATCGCGGG CATCTTGATC
GTGCTGAGCT CGGTGGCTTG CTCCTTGGGT GTCTTCAGCT ACATTGGGTT GCCCTTGACC
CTCATTGTGA TTGAAGTCAT CCCGTTCCTG GTGCTGGCTG TTGGAGTGGA CAACATCTTC
ATTCTGGTGC AGGCCTACCA GAGAGATGAA CGTCTTCAAG GGGAAACCCT GGATCAGCAG
CTGGGCAGGG TCCTAGGAGA AGTGGCTCCC AGTATGTTCC TGTCATCCTT TTCTGAGACT
GTAGCATTTT TCTTAGGAGC ATTGTCCGTG ATGCCAGCCG TGCACACCTT CTCTCTCTTT
GCGGGATTGG CAGTCTTCAT TGACTTTCTT CTGCAGATTA CCTGTTTCGT GAGTCTCTTG
GGGTTAGACA TTAAACGTCA AGAGAAAAAT CGGCTAGACA TCTTTTGCTG TGTCAGAGGT
GCTGAAGATG GAACAAGCGT CCAGGCCTCA GAGAGCTGTT TGTTTCGCTT CTTCAAAAAC
TCCTATTCTC CACTTCTGCT AAAGGACTGG ATGAGACCAA TTGTGATAGC AATATTTGTG
GGTGTTCTGT CATTCAGCAT CGCAGTCCTG AACAAAGTAG ATATTGGATT GGATCAGTCT
CTTTCGATGC CAGATGACTC CTACATGGTG GATTATTTCA AATCCATCAG TCAGTACCTG
CATGCGGGTC CGCCTGTGTA CTTTGTCCTG GAGGAAGGGC ACGACTACAC TTCTTCCAAG
GGGCAGAACA TGGTGTGCGG CGGCATGGGC TGCAACAATG ATTCCCTGGT GCAGCAGATA
TTTAACGCGG CGCAGCTGGA CAACTATACC CGAATAGGCT TCGCCCCCTC GTCCTGGATC
GACGATTATT TCGACTGGGT GAAGCCACAG TCGTCTTGCT GTCGAGTGGA CAATATCACT
GACCAGTTCT GCAATGCTTC AGTGGTTGAC CCTGCCTGCG TTCGCTGCAG GCCTCTGACT
CCGGAAGGCA AACAGAGGCC TCAGGGGGGA GACTTCATGA GATTCCTGCC CATGTTCCTT
TCGGATAACC CTAACCCCAA GTGTGGCAAA GGGGGACATG CTGCCTATAG TTCTGCAGTT
AACATCCTCC TTGGCCATGG CACCAGGGTC GGAGCCACGT ACTTCATGAC CTACCACACC
GTGCTGCAGA CCTCTGCTGA CTTTATTGAC GCTCTGAAGA AAGCCCGACT TATAGCCAGT
AATGTCACCG AAACCATGGG CATTAACGGC AGTGCCTACC GAGTATTTCC TTACAGTGTG
TTTTATGTCT TCTACGAACA GTACCTGACC ATCATTGACG ACACTATCTT CAACCTCGGT
GTGTCCCTGG GCGCGATATT TCTGGTGACC ATGGTCCTCC TGGGCTGTGA GCTCTGGTCT
GCAGTCATCA TGTGTGCCAC CATCGCCATG GTCTTGGTCA ACATGTTTGG AGTTATGTGG
CTCTGGGGCA TCAGTCTGAA CGCTGTATCC TTGGTCAACC TGGTGATGAG CTGTGGCATC
TCCGTGGAGT TCTGCAGCCA CATAACCAGA GCGTTCACGG TGAGCATGAA AGGCAGCCGC
GTGGAGCGCG CGGAAGAGGC ACTTGCCCAC ATGGGCAGCT CCGTGTTCAG TGGAATCACA
CTTACAAAAT TTGGAGGGAT TGTGGTGTTG GCTTTTGCCA AATCTCAAAT TTTCCAGATA
TTCTACTTCA GGATGTATTT GGCCATGGTC TTACTGGGAG CCACTCACGG ATTAATATTT
CTCCCTGTCT TACTCAGTTA CATAGGCCCA TTCAATTCCT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59314
Clone ID Related Accession (Same CDS sequence) XM_011526015
Accession Version XM_011526015.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product Niemann-Pick C1 protein isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_010966.15) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGTACAATG CCTGCCGGGA TGTGGAGGCC CCCTCAAGTA ATGACAAGGC CCTGGGACTC 
CTGTGTGGGA AGGACGCTGA CGCCTGTAAT GCCACCAACT GGATTGAATA CATGTTCAAT
AAGGACAATG GACAGGCACC TTTTACCATC ACTCCTGTGT TTTCAGATTT TCCAGTCCAT
GGGATGGAGC CCATGAACAA TGCCACCAAA GGCTGTGACG AGTCTGTGGA TGAGGTCACA
GCACCATGTA GCTGCCAAGA CTGCTCTATT GTCTGTGGCC CCAAGCCCCA GCCCCCACCT
CCTCCTGCTC CCTGGACGAT CCTTGGCTTG GACGCCATGT ATGTCATCAT GTGGATCACC
TACATGGCGT TTTTGCTTGT GTTTTTTGGA GCATTTTTTG CAGTGTGGTG CTACAGAAAA
CGGTATTTTG TCTCCGAGTA CACTCCCATC GATAGCAATA TAGCTTTTTC TGTTAATGCA
AGTGACAAAG GAACGGCTTG GCTCTTAACC TCCACCTTCC CTTCCTCTCC CGTTCTTCCA
GGAGAGGCGT CCTGCTGTGA CCCTGTCAGC GCAGCATTTG AGGGCTGCTT GAGGCGGCTG
TTCACACGCT GGGGGTCTTT CTGCGTCCGA AACCCTGGCT GTGTCATTTT CTTCTCGCTG
GTCTTCATTA CTGCGTGTTC GTCAGGCCTG GTGTTTGTCC GGGTCACAAC CAATCCAGTT
GACCTCTGGT CAGCCCCCAG CAGCCAGGCT CGCCTGGAAA AAGAGTACTT TGACCAGCAC
TTTGGGCCTT TCTTCCGGAC GGAGCAGCTC ATCATCCGGG CCCCTCTCAC TGACAAACAC
ATTTACCAGC CATACCCTTC GGGAGCTGAT GTACCCTTTG GACCTCCGCT TGACATACAG
ATACTGCACC AGGTTCTTGA CTTACAAATA GCCATCGAAA ACATTACTGC CTCTTATGAC
AATGAGACTG TGACACTTCA AGACATCTGC TTGGCCCCTC TTTCACCGTA TAACACGAAC
TGCACCATTT TGAGTGTGTT AAATTACTTC CAGAACAGCC ATTCCGTGCT GGACCACAAG
AAAGGGGACG ACTTCTTTGT GTATGCCGAT TACCACACGC ACTTTCTGTA CTGCGTACGG
GCTCCTGCCT CTCTGAATGA TACAAGTTTG CTCCATGACC CTTGTCTGGG TACGTTTGGT
GGACCAGTGT TCCCGTGGCT TGTGTTGGGA GGCTATGATG ATCAAAACTA CAATAACGCC
ACTGCCCTTG TGATTACCTT CCCTGTCAAT AATTACTATA ATGATACAGA GAAGCTCCAG
AGGGCCCAGG CCTGGGAAAA AGAGTTTATT AATTTTGTGA AAAACTACAA GAATCCCAAT
CTGACCATTT CCTTCACTGC TGAACGAAGT ATTGAAGATG AACTAAATCG TGAAAGTGAC
AGTGATGTCT TCACCGTTGT AATTAGCTAT GCCATCATGT TTCTATATAT TTCCCTAGCC
TTGGGGCACA TGAAAAGCTG TCGCAGGCTT CTGGTGGATT CGAAGGTCTC ACTAGGCATC
GCGGGCATCT TGATCGTGCT GAGCTCGGTG GCTTGCTCCT TGGGTGTCTT CAGCTACATT
GGGTTGCCCT TGACCCTCAT TGTGATTGAA GTCATCCCGT TCCTGGTGCT GGCTGTTGGA
GTGGACAACA TCTTCATTCT GGTGCAGGCC TACCAGAGAG ATGAACGTCT TCAAGGGGAA
ACCCTGGATC AGCAGCTGGG CAGGGTCCTA GGAGAAGTGG CTCCCAGTAT GTTCCTGTCA
TCCTTTTCTG AGACTGTAGC ATTTTTCTTA GGAGCATTGT CCGTGATGCC AGCCGTGCAC
ACCTTCTCTC TCTTTGCGGG ATTGGCAGTC TTCATTGACT TTCTTCTGCA GATTACCTGT
TTCGTGAGTC TCTTGGGGTT AGACATTAAA CGTCAAGAGA AAAATCGGCT AGACATCTTT
TGCTGTGTCA GAGGTGCTGA AGATGGAACA AGCGTCCAGG CCTCAGAGAG CTGTTTGTTT
CGCTTCTTCA AAAACTCCTA TTCTCCACTT CTGCTAAAGG ACTGGATGAG ACCAATTGTG
ATAGCAATAT TTGTGGGTGT TCTGTCATTC AGCATCGCAG TCCTGAACAA AGTAGATATT
GGATTGGATC AGTCTCTTTC GATGCCAGAT GACTCCTACA TGGTGGATTA TTTCAAATCC
ATCAGTCAGT ACCTGCATGC GGGTCCGCCT GTGTACTTTG TCCTGGAGGA AGGGCACGAC
TACACTTCTT CCAAGGGGCA GAACATGGTG TGCGGCGGCA TGGGCTGCAA CAATGATTCC
CTGGTGCAGC AGATATTTAA CGCGGCGCAG CTGGACAACT ATACCCGAAT AGGCTTCGCC
CCCTCGTCCT GGATCGACGA TTATTTCGAC TGGGTGAAGC CACAGTCGTC TTGCTGTCGA
GTGGACAATA TCACTGACCA GTTCTGCAAT GCTTCAGTGG TTGACCCTGC CTGCGTTCGC
TGCAGGCCTC TGACTCCGGA AGGCAAACAG AGGCCTCAGG GGGGAGACTT CATGAGATTC
CTGCCCATGT TCCTTTCGGA TAACCCTAAC CCCAAGTGTG GCAAAGGGGG ACATGCTGCC
TATAGTTCTG CAGTTAACAT CCTCCTTGGC CATGGCACCA GGGTCGGAGC CACGTACTTC
ATGACCTACC ACACCGTGCT GCAGACCTCT GCTGACTTTA TTGACGCTCT GAAGAAAGCC
CGACTTATAG CCAGTAATGT CACCGAAACC ATGGGCATTA ACGGCAGTGC CTACCGAGTA
TTTCCTTACA GTGTGTTTTA TGTCTTCTAC GAACAGTACC TGACCATCAT TGACGACACT
ATCTTCAACC TCGGTGTGTC CCTGGGCGCG ATATTTCTGG TGACCATGGT CCTCCTGGGC
TGTGAGCTCT GGTCTGCAGT CATCATGTGT GCCACCATCG CCATGGTCTT GGTCAACATG
TTTGGAGTTA TGTGGCTCTG GGGCATCAGT CTGAACGCTG TATCCTTGGT CAACCTGGTG
ATGAGCTGTG GCATCTCCGT GGAGTTCTGC AGCCACATAA CCAGAGCGTT CACGGTGAGC
ATGAAAGGCA GCCGCGTGGA GCGCGCGGAA GAGGCACTTG CCCACATGGG CAGCTCCGTG
TTCAGTGGAA TCACACTTAC AAAATTTGGA GGGATTGTGG TGTTGGCTTT TGCCAAATCT
CAAATTTTCC AGATATTCTA CTTCAGGATG TATTTGGCCA TGGTCTTACT GGGAGCCACT
CACGGATTAA TATTTCTCCC TGTCTTACTC AGTTACATAG CAAAGCTTCT GGAAAGAACT
ATCTACTTGT GCATCTCACA GCACATTCAT TATGCAACCC AGATGGCTCC ACTACAGTGT
CTCATGACCG CCTCAGACAG ATGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011524317.1
CDS877..4323
Misc Feature(1)877..4215(+)
Misc Feature(2)2407..2868(+)
Translation

Target ORF information:

RefSeq Version XM_011526015
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens Niemann-Pick disease, type C1 (NPC1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011526015

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
ATGTACAATG CCTGCCGGGA TGTGGAGGCC CCCTCAAGTA ATGACAAGGC CCTGGGACTC 
CTGTGTGGGA AGGACGCTGA CGCCTGTAAT GCCACCAACT GGATTGAATA CATGTTCAAT
AAGGACAATG GACAGGCACC TTTTACCATC ACTCCTGTGT TTTCAGATTT TCCAGTCCAT
GGGATGGAGC CCATGAACAA TGCCACCAAA GGCTGTGACG AGTCTGTGGA TGAGGTCACA
GCACCATGTA GCTGCCAAGA CTGCTCTATT GTCTGTGGCC CCAAGCCCCA GCCCCCACCT
CCTCCTGCTC CCTGGACGAT CCTTGGCTTG GACGCCATGT ATGTCATCAT GTGGATCACC
TACATGGCGT TTTTGCTTGT GTTTTTTGGA GCATTTTTTG CAGTGTGGTG CTACAGAAAA
CGGTATTTTG TCTCCGAGTA CACTCCCATC GATAGCAATA TAGCTTTTTC TGTTAATGCA
AGTGACAAAG GAACGGCTTG GCTCTTAACC TCCACCTTCC CTTCCTCTCC CGTTCTTCCA
GGAGAGGCGT CCTGCTGTGA CCCTGTCAGC GCAGCATTTG AGGGCTGCTT GAGGCGGCTG
TTCACACGCT GGGGGTCTTT CTGCGTCCGA AACCCTGGCT GTGTCATTTT CTTCTCGCTG
GTCTTCATTA CTGCGTGTTC GTCAGGCCTG GTGTTTGTCC GGGTCACAAC CAATCCAGTT
GACCTCTGGT CAGCCCCCAG CAGCCAGGCT CGCCTGGAAA AAGAGTACTT TGACCAGCAC
TTTGGGCCTT TCTTCCGGAC GGAGCAGCTC ATCATCCGGG CCCCTCTCAC TGACAAACAC
ATTTACCAGC CATACCCTTC GGGAGCTGAT GTACCCTTTG GACCTCCGCT TGACATACAG
ATACTGCACC AGGTTCTTGA CTTACAAATA GCCATCGAAA ACATTACTGC CTCTTATGAC
AATGAGACTG TGACACTTCA AGACATCTGC TTGGCCCCTC TTTCACCGTA TAACACGAAC
TGCACCATTT TGAGTGTGTT AAATTACTTC CAGAACAGCC ATTCCGTGCT GGACCACAAG
AAAGGGGACG ACTTCTTTGT GTATGCCGAT TACCACACGC ACTTTCTGTA CTGCGTACGG
GCTCCTGCCT CTCTGAATGA TACAAGTTTG CTCCATGACC CTTGTCTGGG TACGTTTGGT
GGACCAGTGT TCCCGTGGCT TGTGTTGGGA GGCTATGATG ATCAAAACTA CAATAACGCC
ACTGCCCTTG TGATTACCTT CCCTGTCAAT AATTACTATA ATGATACAGA GAAGCTCCAG
AGGGCCCAGG CCTGGGAAAA AGAGTTTATT AATTTTGTGA AAAACTACAA GAATCCCAAT
CTGACCATTT CCTTCACTGC TGAACGAAGT ATTGAAGATG AACTAAATCG TGAAAGTGAC
AGTGATGTCT TCACCGTTGT AATTAGCTAT GCCATCATGT TTCTATATAT TTCCCTAGCC
TTGGGGCACA TGAAAAGCTG TCGCAGGCTT CTGGTGGATT CGAAGGTCTC ACTAGGCATC
GCGGGCATCT TGATCGTGCT GAGCTCGGTG GCTTGCTCCT TGGGTGTCTT CAGCTACATT
GGGTTGCCCT TGACCCTCAT TGTGATTGAA GTCATCCCGT TCCTGGTGCT GGCTGTTGGA
GTGGACAACA TCTTCATTCT GGTGCAGGCC TACCAGAGAG ATGAACGTCT TCAAGGGGAA
ACCCTGGATC AGCAGCTGGG CAGGGTCCTA GGAGAAGTGG CTCCCAGTAT GTTCCTGTCA
TCCTTTTCTG AGACTGTAGC ATTTTTCTTA GGAGCATTGT CCGTGATGCC AGCCGTGCAC
ACCTTCTCTC TCTTTGCGGG ATTGGCAGTC TTCATTGACT TTCTTCTGCA GATTACCTGT
TTCGTGAGTC TCTTGGGGTT AGACATTAAA CGTCAAGAGA AAAATCGGCT AGACATCTTT
TGCTGTGTCA GAGGTGCTGA AGATGGAACA AGCGTCCAGG CCTCAGAGAG CTGTTTGTTT
CGCTTCTTCA AAAACTCCTA TTCTCCACTT CTGCTAAAGG ACTGGATGAG ACCAATTGTG
ATAGCAATAT TTGTGGGTGT TCTGTCATTC AGCATCGCAG TCCTGAACAA AGTAGATATT
GGATTGGATC AGTCTCTTTC GATGCCAGAT GACTCCTACA TGGTGGATTA TTTCAAATCC
ATCAGTCAGT ACCTGCATGC GGGTCCGCCT GTGTACTTTG TCCTGGAGGA AGGGCACGAC
TACACTTCTT CCAAGGGGCA GAACATGGTG TGCGGCGGCA TGGGCTGCAA CAATGATTCC
CTGGTGCAGC AGATATTTAA CGCGGCGCAG CTGGACAACT ATACCCGAAT AGGCTTCGCC
CCCTCGTCCT GGATCGACGA TTATTTCGAC TGGGTGAAGC CACAGTCGTC TTGCTGTCGA
GTGGACAATA TCACTGACCA GTTCTGCAAT GCTTCAGTGG TTGACCCTGC CTGCGTTCGC
TGCAGGCCTC TGACTCCGGA AGGCAAACAG AGGCCTCAGG GGGGAGACTT CATGAGATTC
CTGCCCATGT TCCTTTCGGA TAACCCTAAC CCCAAGTGTG GCAAAGGGGG ACATGCTGCC
TATAGTTCTG CAGTTAACAT CCTCCTTGGC CATGGCACCA GGGTCGGAGC CACGTACTTC
ATGACCTACC ACACCGTGCT GCAGACCTCT GCTGACTTTA TTGACGCTCT GAAGAAAGCC
CGACTTATAG CCAGTAATGT CACCGAAACC ATGGGCATTA ACGGCAGTGC CTACCGAGTA
TTTCCTTACA GTGTGTTTTA TGTCTTCTAC GAACAGTACC TGACCATCAT TGACGACACT
ATCTTCAACC TCGGTGTGTC CCTGGGCGCG ATATTTCTGG TGACCATGGT CCTCCTGGGC
TGTGAGCTCT GGTCTGCAGT CATCATGTGT GCCACCATCG CCATGGTCTT GGTCAACATG
TTTGGAGTTA TGTGGCTCTG GGGCATCAGT CTGAACGCTG TATCCTTGGT CAACCTGGTG
ATGAGCTGTG GCATCTCCGT GGAGTTCTGC AGCCACATAA CCAGAGCGTT CACGGTGAGC
ATGAAAGGCA GCCGCGTGGA GCGCGCGGAA GAGGCACTTG CCCACATGGG CAGCTCCGTG
TTCAGTGGAA TCACACTTAC AAAATTTGGA GGGATTGTGG TGTTGGCTTT TGCCAAATCT
CAAATTTTCC AGATATTCTA CTTCAGGATG TATTTGGCCA TGGTCTTACT GGGAGCCACT
CACGGATTAA TATTTCTCCC TGTCTTACTC AGTTACATAG CAAAGCTTCT GGAAAGAACT
ATCTACTTGT GCATCTCACA GCACATTCAT TATGCAACCC AGATGGCTCC ACTACAGTGT
CTCATGACCG CCTCAGACAG ATGTTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu27327
Clone ID Related Accession (Same CDS sequence) NM_000271
Accession Version NM_000271.4 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 3837bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product Niemann-Pick C1 protein precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR159421.1, AF002020.1, AB209048.1 and BC063302.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2009 this sequence version replaced gi:133930789. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF002020.1, AB209048.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGGGCCAT CAGTAAATAA AGCCAAAAGT
TGTGCCACTG AAGAGCGATA CAAAGGAACA GAGCGCGAAC GGCTTCTAAA TTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000262.2
CDS275..4111
Misc Feature(1)347..4030(+)
Misc Feature(2)596..598(+)
Misc Feature(3)797..889(+)
Misc Feature(4)1082..1144(+)
Misc Feature(5)1325..1387(+)
Misc Feature(6)2135..2197(+)
Misc Feature(7)2219..2680(+)
Misc Feature(8)2237..2299(+)
Misc Feature(9)2306..2368(+)
Misc Feature(10)2477..2539(+)
Misc Feature(11)2552..2614(+)
Misc Feature(12)2771..2833(+)
Misc Feature(13)3569..3631(+)
Misc Feature(14)3647..3709(+)
Misc Feature(15)3713..3775(+)
Misc Feature(16)3860..3922(+)
Misc Feature(17)3956..4018(+)
Misc Feature(18)4097..4108(+)
Exon (1)1..331
Gene:NPC1
Gene Synonym:
Exon (2)332..454
Gene:NPC1
Gene Synonym:
Exon (3)455..561
Gene:NPC1
Gene Synonym:
Exon (4)562..737
Gene:NPC1
Gene Synonym:
Exon (5)738..905
Gene:NPC1
Gene Synonym:
Exon (6)906..1155
Gene:NPC1
Gene Synonym:
Exon (7)1156..1229
Gene:NPC1
Gene Synonym:
Exon (8)1230..1600
Gene:NPC1
Gene Synonym:
Exon (9)1601..1827
Gene:NPC1
Gene Synonym:
Exon (10)1828..1928
Gene:NPC1
Gene Synonym:
Exon (11)1929..2031
Gene:NPC1
Gene Synonym:
Exon (12)2032..2221
Gene:NPC1
Gene Synonym:
Exon (13)2222..2404
Gene:NPC1
Gene Synonym:
Exon (14)2405..2519
Gene:NPC1
Gene Synonym:
Exon (15)2520..2647
Gene:NPC1
Gene Synonym:
Exon (16)2648..2788
Gene:NPC1
Gene Synonym:
Exon (17)2789..2878
Gene:NPC1
Gene Synonym:
Exon (18)2879..3069
Gene:NPC1
Gene Synonym:
Exon (19)3070..3185
Gene:NPC1
Gene Synonym:
Exon (20)3186..3315
Gene:NPC1
Gene Synonym:
Exon (21)3316..3519
Gene:NPC1
Gene Synonym:
Exon (22)3520..3751
Gene:NPC1
Gene Synonym:
Exon (23)3752..3865
Gene:NPC1
Gene Synonym:
Exon (24)3866..4028
Gene:NPC1
Gene Synonym:
Exon (25)4029..4814
Gene:NPC1
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000271
Organism Homo sapiens (human)
Definition Homo sapiens Niemann-Pick disease, type C1 (NPC1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000271

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
ATGACCGCTC GCGGCCTGGC CCTTGGCCTC CTCCTGCTGC TACTGTGTCC AGCGCAGGTG 
TTTTCACAGT CCTGTGTTTG GTATGGAGAG TGTGGAATTG CATATGGGGA CAAGAGGTAC
AATTGCGAAT ATTCTGGCCC ACCAAAACCA TTGCCAAAGG ATGGATATGA CTTAGTGCAG
GAACTCTGTC CAGGATTCTT CTTTGGCAAT GTCAGTCTCT GTTGTGATGT TCGGCAGCTT
CAGACACTAA AAGACAACCT GCAGCTGCCT CTACAGTTTC TGTCCAGATG TCCATCCTGT
TTTTATAACC TACTGAACCT GTTTTGTGAG CTGACATGTA GCCCTCGACA GAGTCAGTTT
TTGAATGTTA CAGCTACTGA AGATTATGTT GATCCTGTTA CAAACCAGAC GAAAACAAAT
GTGAAAGAGT TACAATACTA CGTCGGACAG AGTTTTGCCA ATGCAATGTA CAATGCCTGC
CGGGATGTGG AGGCCCCCTC AAGTAATGAC AAGGCCCTGG GACTCCTGTG TGGGAAGGAC
GCTGACGCCT GTAATGCCAC CAACTGGATT GAATACATGT TCAATAAGGA CAATGGACAG
GCACCTTTTA CCATCACTCC TGTGTTTTCA GATTTTCCAG TCCATGGGAT GGAGCCCATG
AACAATGCCA CCAAAGGCTG TGACGAGTCT GTGGATGAGG TCACAGCACC ATGTAGCTGC
CAAGACTGCT CTATTGTCTG TGGCCCCAAG CCCCAGCCCC CACCTCCTCC TGCTCCCTGG
ACGATCCTTG GCTTGGACGC CATGTATGTC ATCATGTGGA TCACCTACAT GGCGTTTTTG
CTTGTGTTTT TTGGAGCATT TTTTGCAGTG TGGTGCTACA GAAAACGGTA TTTTGTCTCC
GAGTACACTC CCATCGATAG CAATATAGCT TTTTCTGTTA ATGCAAGTGA CAAAGGAGAG
GCGTCCTGCT GTGACCCTGT CAGCGCAGCA TTTGAGGGCT GCTTGAGGCG GCTGTTCACA
CGCTGGGGGT CTTTCTGCGT CCGAAACCCT GGCTGTGTCA TTTTCTTCTC GCTGGTCTTC
ATTACTGCGT GTTCGTCAGG CCTGGTGTTT GTCCGGGTCA CAACCAATCC AGTTGACCTC
TGGTCAGCCC CCAGCAGCCA GGCTCGCCTG GAAAAAGAGT ACTTTGACCA GCACTTTGGG
CCTTTCTTCC GGACGGAGCA GCTCATCATC CGGGCCCCTC TCACTGACAA ACACATTTAC
CAGCCATACC CTTCGGGAGC TGATGTACCC TTTGGACCTC CGCTTGACAT ACAGATACTG
CACCAGGTTC TTGACTTACA AATAGCCATC GAAAACATTA CTGCCTCTTA TGACAATGAG
ACTGTGACAC TTCAAGACAT CTGCTTGGCC CCTCTTTCAC CGTATAACAC GAACTGCACC
ATTTTGAGTG TGTTAAATTA CTTCCAGAAC AGCCATTCCG TGCTGGACCA CAAGAAAGGG
GACGACTTCT TTGTGTATGC CGATTACCAC ACGCACTTTC TGTACTGCGT ACGGGCTCCT
GCCTCTCTGA ATGATACAAG TTTGCTCCAT GACCCTTGTC TGGGTACGTT TGGTGGACCA
GTGTTCCCGT GGCTTGTGTT GGGAGGCTAT GATGATCAAA ACTACAATAA CGCCACTGCC
CTTGTGATTA CCTTCCCTGT CAATAATTAC TATAATGATA CAGAGAAGCT CCAGAGGGCC
CAGGCCTGGG AAAAAGAGTT TATTAATTTT GTGAAAAACT ACAAGAATCC CAATCTGACC
ATTTCCTTCA CTGCTGAACG AAGTATTGAA GATGAACTAA ATCGTGAAAG TGACAGTGAT
GTCTTCACCG TTGTAATTAG CTATGCCATC ATGTTTCTAT ATATTTCCCT AGCCTTGGGG
CACATGAAAA GCTGTCGCAG GCTTCTGGTG GATTCGAAGG TCTCACTAGG CATCGCGGGC
ATCTTGATCG TGCTGAGCTC GGTGGCTTGC TCCTTGGGTG TCTTCAGCTA CATTGGGTTG
CCCTTGACCC TCATTGTGAT TGAAGTCATC CCGTTCCTGG TGCTGGCTGT TGGAGTGGAC
AACATCTTCA TTCTGGTGCA GGCCTACCAG AGAGATGAAC GTCTTCAAGG GGAAACCCTG
GATCAGCAGC TGGGCAGGGT CCTAGGAGAA GTGGCTCCCA GTATGTTCCT GTCATCCTTT
TCTGAGACTG TAGCATTTTT CTTAGGAGCA TTGTCCGTGA TGCCAGCCGT GCACACCTTC
TCTCTCTTTG CGGGATTGGC AGTCTTCATT GACTTTCTTC TGCAGATTAC CTGTTTCGTG
AGTCTCTTGG GGTTAGACAT TAAACGTCAA GAGAAAAATC GGCTAGACAT CTTTTGCTGT
GTCAGAGGTG CTGAAGATGG AACAAGCGTC CAGGCCTCAG AGAGCTGTTT GTTTCGCTTC
TTCAAAAACT CCTATTCTCC ACTTCTGCTA AAGGACTGGA TGAGACCAAT TGTGATAGCA
ATATTTGTGG GTGTTCTGTC ATTCAGCATC GCAGTCCTGA ACAAAGTAGA TATTGGATTG
GATCAGTCTC TTTCGATGCC AGATGACTCC TACATGGTGG ATTATTTCAA ATCCATCAGT
CAGTACCTGC ATGCGGGTCC GCCTGTGTAC TTTGTCCTGG AGGAAGGGCA CGACTACACT
TCTTCCAAGG GGCAGAACAT GGTGTGCGGC GGCATGGGCT GCAACAATGA TTCCCTGGTG
CAGCAGATAT TTAACGCGGC GCAGCTGGAC AACTATACCC GAATAGGCTT CGCCCCCTCG
TCCTGGATCG ACGATTATTT CGACTGGGTG AAGCCACAGT CGTCTTGCTG TCGAGTGGAC
AATATCACTG ACCAGTTCTG CAATGCTTCA GTGGTTGACC CTGCCTGCGT TCGCTGCAGG
CCTCTGACTC CGGAAGGCAA ACAGAGGCCT CAGGGGGGAG ACTTCATGAG ATTCCTGCCC
ATGTTCCTTT CGGATAACCC TAACCCCAAG TGTGGCAAAG GGGGACATGC TGCCTATAGT
TCTGCAGTTA ACATCCTCCT TGGCCATGGC ACCAGGGTCG GAGCCACGTA CTTCATGACC
TACCACACCG TGCTGCAGAC CTCTGCTGAC TTTATTGACG CTCTGAAGAA AGCCCGACTT
ATAGCCAGTA ATGTCACCGA AACCATGGGC ATTAACGGCA GTGCCTACCG AGTATTTCCT
TACAGTGTGT TTTATGTCTT CTACGAACAG TACCTGACCA TCATTGACGA CACTATCTTC
AACCTCGGTG TGTCCCTGGG CGCGATATTT CTGGTGACCA TGGTCCTCCT GGGCTGTGAG
CTCTGGTCTG CAGTCATCAT GTGTGCCACC ATCGCCATGG TCTTGGTCAA CATGTTTGGA
GTTATGTGGC TCTGGGGCAT CAGTCTGAAC GCTGTATCCT TGGTCAACCT GGTGATGAGC
TGTGGCATCT CCGTGGAGTT CTGCAGCCAC ATAACCAGAG CGTTCACGGT GAGCATGAAA
GGCAGCCGCG TGGAGCGCGC GGAAGAGGCA CTTGCCCACA TGGGCAGCTC CGTGTTCAGT
GGAATCACAC TTACAAAATT TGGAGGGATT GTGGTGTTGG CTTTTGCCAA ATCTCAAATT
TTCCAGATAT TCTACTTCAG GATGTATTTG GCCATGGTCT TACTGGGAGC CACTCACGGA
TTAATATTTC TCCCTGTCTT ACTCAGTTAC ATAGGGCCAT CAGTAAATAA AGCCAAAAGT
TGTGCCACTG AAGAGCGATA CAAAGGAACA GAGCGCGAAC GGCTTCTAAA TTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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