×

CISD2 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol CISD2
Entrez Gene ID 493856
Full Name CDGSH iron sulfur domain 2
Synonyms ERIS,Miner1,NAF-1,WFS2,ZCD2
General protein information
Preferred Names

CDGSH iron sulfur domain 2

Names

CDGSH iron-sulfur domain-containing protein 2
endoplasmic reticulum intermembrane small protein
mitoNEET-related 1 protein
nutrient-deprivation autophagy factor-1
zinc finger, CDGSH-type domain 2

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

4

4q24

Summary The protein encoded by this gene is a zinc finger protein that localizes to the endoplasmic reticulum. The encoded protein binds an iron/sulfur cluster and may be involved in calcium homeostasis. Defects in this gene are a cause of Wolfram syndrome 2. [provided by RefSeq, Mar 2011].
Disorder MIM:

611507

Disorder Html: Wolfram syndrome 2, 604928 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001008388.4 NP_001008389.1 CDGSH iron-sulfur domain-containing protein 2



Homo sapiens (human) CISD2 NP_001008389.1
Rattus norvegicus (Norway rat) Cisd2 NP_001178537.1
Danio rerio (zebrafish) cisd2 NP_956677.1
Xenopus tropicalis (tropical clawed frog) cisd2 NP_001011479.1
Pan troglodytes (chimpanzee) CISD2 XP_003310469.1
Mus musculus (house mouse) Cisd2 NP_080178.1
Gallus gallus (chicken) CISD2 XP_420668.1
Drosophila melanogaster (fruit fly) CG1458 NP_651684.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP004484 XP_313782.3
Bos taurus (cattle) CISD2 NP_001073807.1
Macaca mulatta (rhesus monkey) LOC100429989 XP_002804204.1


Related articles in PubMed

NAF-1 and mitoNEET are central to human breast cancer proliferation by maintaining mitochondrial homeostasis and promoting tumor growth.
Sohn YS, Tamir S, Song L, Michaeli D, Matouk I, Conlan AR, Harir Y, Holt SH, Shulaev V, Paddock ML, Hochberg A, Cabanchick IZ, Onuchic JN, Jennings PA, Nechushtai R, Mittler R
Proceedings of the National Academy of Sciences of the United States of America110(36)14676-81(2013 Sep)

A role for the CISD2 gene in lifespan control and human disease.
Chen YF, Wu CY, Kirby R, Kao CH, Tsai TF
Annals of the New York Academy of Sciences120158-64(2010 Jul)

Antagonism of Beclin 1-dependent autophagy by BCL-2 at the endoplasmic reticulum requires NAF-1.
Chang NC, Nguyen M, Germain M, Shore GC
The EMBO journal29(3)606-18(2010 Feb)

Crystal structure of Miner1: The redox-active 2Fe-2S protein causative in Wolfram Syndrome 2.
Conlan AR, Axelrod HL, Cohen AE, Abresch EC, Zuris J, Yee D, Nechushtai R, Jennings PA, Paddock ML
Journal of molecular biology392(1)143-53(2009 Sep)

A homozygous mutation in a novel zinc-finger protein, ERIS, is responsible for Wolfram syndrome 2.
Amr S, Heisey C, Zhang M, Xia XJ, Shows KH, Ajlouni K, Pandya A, Satin LS, El-Shanti H, Shiang R
American journal of human genetics81(4)673-83(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NAF-1 is a major player in the metabolic regulation of breast cancer cells.
Title: Activation of apoptosis in NAF-1-deficient human epithelial breast cancer cells.

High CISD2 expression was significantly associated with gastric cancer.
Title: Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway.

CISD2 exerts anti-apoptotic and anti-inflammatory effects in neural cells; and (2) curcumin can attenuate the downregulation of CISD2 in SCI and LPS-treated astrocytes.
Title: CISD2 serves a novel role as a suppressor of nitric oxide signalling and curcumin increases CISD2 expression in spinal cord injuries.

The findings suggested that CISD2 haplotype-tagging single nucleotide polymorphisms are not associated with Alzheimer's disease risk.
Title: Sequence variants of the aging gene CISD2 and the risk for Alzheimer's disease.

CISD2 protein may serve as a candidate prognostic marker and a novel therapeutic target for hepatocellular carcinoma (HCC) and play an important role in promoting proliferation and enhanced progression of HCC.
Title: CISD2 associated with proliferation indicates negative prognosis in patients with hepatocellular carcinoma.

The following CISD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CISD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu23185 NM_001008388.4
Latest version!
Homo sapiens CDGSH iron sulfur domain 2 (CISD2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu23185
Clone ID Related Accession (Same CDS sequence) NM_001008388.4
Accession Version NM_001008388.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 408bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2015-03-15
Organism Homo sapiens(Human)
Product CDGSH iron-sulfur domain-containing protein 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC018797.4, AK292134.1, BX537971.1 and AC083826.7. This sequence is a reference standard in the RefSeqGene project. On Jul 3, 2010 this sequence version replaced NM_001008388.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DN993861.1, BX537971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
ATGGTGCTGG AGAGCGTGGC CCGTATCGTG AAGGTGCAGC TCCCTGCATA TCTGAAGCGG 
CTCCCAGTCC CTGAAAGCAT TACCGGGTTC GCTAGGCTCA CAGTTTCAGA ATGGCTTCGG
TTATTGCCTT TCCTTGGTGT ACTCGCACTT CTTGGCTACC TTGCAGTTCG TCCATTCCTC
CCGAAGAAGA AACAACAGAA GGATAGCTTG ATTAATCTTA AAATACAAAA GGAAAATCCG
AAAGTAGTGA ATGAAATAAA CATTGAAGAT TTGTGTCTTA CTAAAGCAGC TTATTGTAGG
TGTTGGCGTT CTAAAACGTT TCCTGCCTGC GATGGTTCAC ATAATAAACA CAATGAATTG
ACAGGAGATA ATGTGGGTCC ACTAATACTG AAGAAGAAAG AAGTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001008389.1
CDS108..515
Misc Feature(1)114..305
Misc Feature(2)219..287
Misc Feature(3)348..464
Exon (1)1..210
Exon (2)211..425
Exon (3)426..5892
Translation

Target ORF information:

RefSeq Version NM_001008388.4
Organism Homo sapiens(Human)
Definition Homo sapiens CDGSH iron sulfur domain 2 (CISD2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001008388.4

ORF Insert Sequence:

1
61
121
181
241
301
361
ATGGTGCTGG AGAGCGTGGC CCGTATCGTG AAGGTGCAGC TCCCTGCATA TCTGAAGCGG 
CTCCCAGTCC CTGAAAGCAT TACCGGGTTC GCTAGGCTCA CAGTTTCAGA ATGGCTTCGG
TTATTGCCTT TCCTTGGTGT ACTCGCACTT CTTGGCTACC TTGCAGTTCG TCCATTCCTC
CCGAAGAAGA AACAACAGAA GGATAGCTTG ATTAATCTTA AAATACAAAA GGAAAATCCG
AAAGTAGTGA ATGAAATAAA CATTGAAGAT TTGTGTCTTA CTAAAGCAGC TTATTGTAGG
TGTTGGCGTT CTAAAACGTT TCCTGCCTGC GATGGTTCAC ATAATAAACA CAATGAATTG
ACAGGAGATA ATGTGGGTCC ACTAATACTG AAGAAGAAAG AAGTATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

NAF-1 and mitoNEET are central to human breast cancer proliferation by maintaining mitochondrial homeostasis and promoting tumor growth.
Proceedings of the National Academy of Sciences of the United States of America110(36)14676-81(2013 Sep)
Sohn YS,Tamir S,Song L,Michaeli D,Matouk I,Conlan AR,Harir Y,Holt SH,Shulaev V,Paddock ML,Hochberg A,Cabanchick IZ,Onuchic JN,Jennings PA,Nechushtai R,Mittler R


book

A role for the CISD2 gene in lifespan control and human disease.
Annals of the New York Academy of Sciences120158-64(2010 Jul)
Chen YF,Wu CY,Kirby R,Kao CH,Tsai TF


book

Antagonism of Beclin 1-dependent autophagy by BCL-2 at the endoplasmic reticulum requires NAF-1.
The EMBO journal29(3)606-18(2010 Feb)
Chang NC,Nguyen M,Germain M,Shore GC


book

Crystal structure of Miner1: The redox-active 2Fe-2S protein causative in Wolfram Syndrome 2.
Journal of molecular biology392(1)143-53(2009 Sep)
Conlan AR,Axelrod HL,Cohen AE,Abresch EC,Zuris J,Yee D,Nechushtai R,Jennings PA,Paddock ML


book

A homozygous mutation in a novel zinc-finger protein, ERIS, is responsible for Wolfram syndrome 2.
American journal of human genetics81(4)673-83(2007 Oct)
Amr S,Heisey C,Zhang M,Xia XJ,Shows KH,Ajlouni K,Pandya A,Satin LS,El-Shanti H,Shiang R


 
*
*
*
*