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ALDH7A1 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol ALDH7A1
Entrez Gene ID 501
Full Name aldehyde dehydrogenase 7 family member A1
Synonyms ATQ1,EPD,PDE
General protein information
Preferred Names

aldehyde dehydrogenase 7 family member A1

Names

alpha-aminoadipic semialdehyde dehydrogenase
26g turgor protein homolog
P6c dehydrogenase
alpha-AASA dehydrogenase
antiquitin-1
betaine aldehyde dehydrogenase
delta1-piperideine-6-carboxylate dehydrogenase

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

5

5q23.2

Summary The protein encoded by this gene is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. It is also involved in lysine catabolism that is known to occur in the mitochondrial matrix. Recent reports show that this protein is found both in the cytosol and the mitochondria, and the two forms likely arise from the use of alternative translation initiation sites. An additional variant encoding a different isoform has also been found for this gene. Mutations in this gene are associated with pyridoxine-dependent epilepsy. Several related pseudogenes have also been identified. [provided by RefSeq, Jan 2011].
Disorder MIM:

107323

Disorder Html: Epilepsy, pyridoxine-dependent, 266100 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001182.4 NP_001173.2 alpha-aminoadipic semialdehyde dehydrogenase isoform 1 precursor
NM_001201377.1 NP_001188306.1 alpha-aminoadipic semialdehyde dehydrogenase isoform 2
XM_017009493.1 XP_016864982.1 alpha-aminoadipic semialdehyde dehydrogenase isoform X1
XM_011543417.2 XP_011541719.1 alpha-aminoadipic semialdehyde dehydrogenase isoform X1
NM_001202404.1 NP_001189333.2 alpha-aminoadipic semialdehyde dehydrogenase isoform 3



Bos taurus (cattle) ALDH7A1 NP_001039434.2
Oryza sativa (rice) Os09g0440300 NP_001063281.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP000881 XP_316857.3
Macaca mulatta (rhesus monkey) ALDH7A1 XP_001095681.2
Homo sapiens (human) ALDH7A1 NP_001173.2
Pan troglodytes (chimpanzee) ALDH7A1 XP_001157473.2
Gallus gallus (chicken) ALDH7A1 XP_424422.2
Danio rerio (zebrafish) aldh7a1 NP_997889.1
Drosophila melanogaster (fruit fly) CG9629 NP_649099.1
Xenopus tropicalis (tropical clawed frog) aldh7a1 NP_001016377.1
Canis lupus familiaris (dog) ALDH7A1 XP_538607.3
Mus musculus (house mouse) Aldh7a1 NP_613066.2
Rattus norvegicus (Norway rat) Aldh7a1 NP_001258034.1
Caenorhabditis elegans (roundworm) alh-9 NP_498263.2
Arabidopsis thaliana (thale cress) ALDH7B4 NP_175812.1


Related articles in PubMed

Intragenic deletions of ALDH7A1 in pyridoxine-dependent epilepsy caused by Alu-Alu recombination.
Mefford HC, Zemel M, Geraghty E, Cook J, Clayton PT, Paul K, Plecko B, Mills PB, Nordli DR Jr, Gospe SM Jr
Neurology85(9)756-62(2015 Sep)

Structural Basis of Substrate Recognition by Aldehyde Dehydrogenase 7A1.
Luo M, Tanner JJ
Biochemistry54(35)5513-22(2015 Sep)

The ALDH7A1 genetic polymorphisms contribute to development of esophageal squamous cell carcinoma.
Wang H, Tong L, Wei J, Pan W, Li L, Ge Y, Zhou L, Yuan Q, Zhou C, Yang M
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine35(12)12665-70(2014 Dec)

Clinical diagnosis, treatment, and ALDH7A1 mutations in pyridoxine-dependent epilepsy in three Chinese infants.
Yang Z, Yang X, Wu Y, Wang J, Zhang Y, Xiong H, Jiang Y, Qin J
PloS one9(3)e92803(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Direct sequencing of the ALDH7A1 gene revealed one novel (c.297delG, p.Trp99*) and two already reported (c.328C>T, p.Arg110*; c.584A>G, p.Asn195Ser) mutations
Title: First cases of pyridoxine-dependent epilepsy in Bulgaria: novel mutation in the ALDH7A1 gene.

This study found five novel mutations of ALDH7A1 gene in pyridoxin dependent epilepsy.
Title: A cohort study of pyridoxine-dependent epilepsy and high prevalence of splice site IVS11+1G>A mutation in Chinese patients.

Using a custom array, study identified heterozygous intragenic deletions in the ALDH7A1 gene in 5 of 6 patients with pyridoxine-dependent epilepsy and positive biomarkers who had only a single mutation identified by conventional sequence analysis
Title: Intragenic deletions of ALDH7A1 in pyridoxine-dependent epilepsy caused by Alu-Alu recombination.

Binding to ALDH7A1 is associated with movement of the C-terminus into the active site which stabilizes the substrate anchor loop.
Title: Structural Basis of Substrate Recognition by Aldehyde Dehydrogenase 7A1.

We present the clinical and molecular genetic findings of two patients with c.1597_1597delG mutations in ALDH7A1 gene.
Title: Pyridoxine-dependent epilepsy in two Turkish patients in Turkey and review of the literature.

The following ALDH7A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALDH7A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu22561
NM_001182.4
Latest version!
Homo sapiens aldehyde dehydrogenase 7 family member A1 (ALDH7A1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu22668
NM_001201377.1
Latest version!
Homo sapiens aldehyde dehydrogenase 7 family member A1 (ALDH7A1), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu56423 XM_017009493.1
Latest version!
Homo sapiens aldehyde dehydrogenase 7 family member A1 (ALDH7A1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu56423 XM_011543417.2
Latest version!
Homo sapiens aldehyde dehydrogenase 7 family member A1 (ALDH7A1), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
OHu100433 NM_001202404.1
Latest version!
Homo sapiens aldehyde dehydrogenase 7 family member A1 (ALDH7A1), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu22561
Clone ID Related Accession (Same CDS sequence) NM_001182.4
Accession Version NM_001182.4 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1620bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-08-28
Organism Homo sapiens(Human)
Product alpha-aminoadipic semialdehyde dehydrogenase isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC375277.1, BC071712.1, AK021800.1, AC093535.2, DB501575.1, BU153725.1, CD678674.1, BG680326.1, AK123774.1 and AI039375.1. This sequence is a reference standard in the RefSeqGene project. On Jan 19, 2011 this sequence version replaced NM_001182.3. Transcript Variant: This variant (1) encodes two isoforms resulting from the use of alternative in-frame translation initiation codons. The longer isoform (1) is derived from an upstream AUG (at nt 193-195), while the shorter isoform (2) is derived from a downstream AUG (at nt 277-279). This RefSeq represents the longer isoform, which resides in the mitochondria (PMIDs: 20207735 and 19885858). Sequence Note: This Refseq, containing three potential in-frame translation initiation codons (all with weak Kozak signals), is annotated with a CDS starting from a downstream start codon (at nt 193-195) based on better conservation, N-terminal consistency with homologous proteins, and the presence of a transit peptide, which is essential for the localization of this isoform in the mitochondria (PMIDs: 20207735 and 19885858), and is consistent with the function of this gene in lysine catabolism (which is known to occur in the mitochondria). The use of an upstream start codon (at nt 112-114) that is present in only a subset of higher mammals, would increase the protein length by 27 aa. A shorter, soluble isoform resulting from the use of another downstream start codon (at nt 277-279) is represented in a separate RefSeq (NM_001201377.1). This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071712.1, S74728.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:19885858) gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTGGCGCC TTCCTCGCGC GCTGTGTGTG CACGCTGCAA AGACCAGCAA GCTCTCTGGA 
CCTTGGAGCA GGCCTGCCGC CTTCATGTCC ACTCTCCTCA TCAATCAGCC CCAGTATGCG
TGGCTGAAAG AGCTGGGGCT CCGCGAGGAA AACGAGGGCG TGTATAATGG AAGCTGGGGA
GGCCGGGGAG AGGTTATTAC GACCTATTGC CCTGCTAACA ACGAGCCAAT AGCAAGAGTC
CGACAGGCCA GTGTGGCAGA CTATGAAGAA ACTGTAAAGA AAGCAAGAGA AGCATGGAAA
ATCTGGGCAG ATATTCCTGC TCCAAAACGA GGAGAAATAG TAAGACAGAT TGGCGATGCC
TTGCGGGAGA AGATCCAAGT ACTAGGAAGC TTGGTGTCTT TGGAGATGGG GAAAATCTTA
GTGGAAGGTG TGGGTGAAGT TCAGGAGTAT GTGGATATCT GTGACTATGC TGTTGGTTTA
TCAAGGATGA TTGGAGGACC TATCTTGCCT TCTGAAAGAT CTGGCCATGC ACTGATTGAG
CAGTGGAATC CCGTAGGCCT GGTTGGAATC ATCACGGCAT TCAATTTCCC TGTGGCAGTG
TATGGTTGGA ACAACGCCAT CGCCATGATC TGTGGAAATG TCTGCCTCTG GAAAGGAGCT
CCAACCACTT CCCTCATTAG TGTGGCTGTC ACAAAGATAA TAGCCAAGGT TCTGGAGGAC
AACAAGCTGC CTGGTGCAAT TTGTTCCTTG ACTTGTGGTG GAGCAGATAT TGGCACAGCA
ATGGCCAAAG ATGAACGAGT GAACCTGCTG TCCTTCACTG GGAGCACTCA GGTGGGAAAA
CAGGTGGGCC TGATGGTGCA GGAGAGGTTT GGGAGAAGTC TGTTGGAACT TGGAGGAAAC
AATGCCATTA TTGCCTTTGA AGATGCAGAC CTCAGCTTAG TTGTTCCATC AGCTCTCTTC
GCTGCTGTGG GAACAGCTGG CCAGAGGTGT ACCACTGCGA GGCGACTGTT TATACATGAA
AGCATCCATG ATGAGGTTGT AAACAGACTT AAAAAGGCCT ATGCACAGAT CCGAGTTGGG
AACCCATGGG ACCCTAATGT TCTCTATGGG CCACTCCACA CCAAGCAGGC AGTGAGCATG
TTTCTTGGAG CAGTGGAAGA AGCAAAGAAA GAAGGTGGCA CAGTGGTCTA TGGGGGCAAG
GTTATGGATC GCCCTGGAAA TTATGTAGAA CCGACAATTG TGACAGGTCT TGGCCACGAT
GCGTCCATTG CACACACAGA GACTTTTGCT CCGATTCTCT ATGTCTTTAA ATTCAAGAAT
GAAGAAGAGG TCTTTGCATG GAATAATGAA GTAAAACAGG GACTTTCAAG TAGCATCTTT
ACCAAAGATC TGGGCAGAAT CTTTCGCTGG CTTGGACCTA AAGGATCAGA CTGTGGCATT
GTAAATGTCA ACATTCCAAC AAGTGGGGCT GAGATTGGAG GTGCCTTTGG AGGAGAAAAG
CACACTGGTG GTGGCAGGGA GTCTGGCAGT GATGCCTGGA AACAGTACAT GAGAAGGTCT
ACTTGTACTA TCAACTACAG TAAAGACCTT CCTCTGGCCC AAGGAATCAA GTTTCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001173.2
CDS193..1812
Misc Feature(1)112..114
Misc Feature(2)349..1773
Misc Feature(3)472..474
Misc Feature(4)472..474
Misc Feature(5)order(490..492,508..510,517..519,535..537,556..558,673..678,682..684,688..690,694..696,700..711,730..735,739..741,1030..1032,1039..1044,1051..1053,1066..1071,1150..1152,1258..1260,1294..1296,1516..1518,1585..1587,1603..1608,1618..1620,1627..1629,1633..1644,1660..1665,1675..1677,1687..1689,1708..1710,1720..1725,1732..1734,1744..1761,1765..1770)
Misc Feature(6)order(766..777,844..846,850..855,964..969,1006..1017,1024..1026,1033..1035,1078..1086,1180..1182,1471..1473,1678..1680)
Misc Feature(7)order(775..777,1078..1080,1171..1173,1180..1182)
Misc Feature(8)775..777
Misc Feature(9)1576..1578
Misc Feature(10)1690..1692
Misc Feature(11)1801..1803
Misc Feature(12)277..279
Exon (1)1..384
Exon (2)385..438
Exon (3)439..504
Exon (4)505..585
Exon (5)586..709
Exon (6)710..842
Exon (7)843..887
Exon (8)888..965
Exon (9)966..1063
Exon (10)1064..1105
Exon (11)1106..1200
Exon (12)1201..1285
Exon (13)1286..1392
Exon (14)1393..1509
Exon (15)1510..1607
Exon (16)1608..1681
Exon (17)1682..1757
Exon (18)1758..4936
Translation

Target ORF information:

RefSeq Version NM_001182.4
Organism Homo sapiens(Human)
Definition Homo sapiens aldehyde dehydrogenase 7 family member A1 (ALDH7A1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001182.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGTGGCGCC TTCCTCGCGC GCTGTGTGTG CACGCTGCAA AGACCAGCAA GCTCTCTGGA 
CCTTGGAGCA GGCCTGCCGC CTTCATGTCC ACTCTCCTCA TCAATCAGCC CCAGTATGCG
TGGCTGAAAG AGCTGGGGCT CCGCGAGGAA AACGAGGGCG TGTATAATGG AAGCTGGGGA
GGCCGGGGAG AGGTTATTAC GACCTATTGC CCTGCTAACA ACGAGCCAAT AGCAAGAGTC
CGACAGGCCA GTGTGGCAGA CTATGAAGAA ACTGTAAAGA AAGCAAGAGA AGCATGGAAA
ATCTGGGCAG ATATTCCTGC TCCAAAACGA GGAGAAATAG TAAGACAGAT TGGCGATGCC
TTGCGGGAGA AGATCCAAGT ACTAGGAAGC TTGGTGTCTT TGGAGATGGG GAAAATCTTA
GTGGAAGGTG TGGGTGAAGT TCAGGAGTAT GTGGATATCT GTGACTATGC TGTTGGTTTA
TCAAGGATGA TTGGAGGACC TATCTTGCCT TCTGAAAGAT CTGGCCATGC ACTGATTGAG
CAGTGGAATC CCGTAGGCCT GGTTGGAATC ATCACGGCAT TCAATTTCCC TGTGGCAGTG
TATGGTTGGA ACAACGCCAT CGCCATGATC TGTGGAAATG TCTGCCTCTG GAAAGGAGCT
CCAACCACTT CCCTCATTAG TGTGGCTGTC ACAAAGATAA TAGCCAAGGT TCTGGAGGAC
AACAAGCTGC CTGGTGCAAT TTGTTCCTTG ACTTGTGGTG GAGCAGATAT TGGCACAGCA
ATGGCCAAAG ATGAACGAGT GAACCTGCTG TCCTTCACTG GGAGCACTCA GGTGGGAAAA
CAGGTGGGCC TGATGGTGCA GGAGAGGTTT GGGAGAAGTC TGTTGGAACT TGGAGGAAAC
AATGCCATTA TTGCCTTTGA AGATGCAGAC CTCAGCTTAG TTGTTCCATC AGCTCTCTTC
GCTGCTGTGG GAACAGCTGG CCAGAGGTGT ACCACTGCGA GGCGACTGTT TATACATGAA
AGCATCCATG ATGAGGTTGT AAACAGACTT AAAAAGGCCT ATGCACAGAT CCGAGTTGGG
AACCCATGGG ACCCTAATGT TCTCTATGGG CCACTCCACA CCAAGCAGGC AGTGAGCATG
TTTCTTGGAG CAGTGGAAGA AGCAAAGAAA GAAGGTGGCA CAGTGGTCTA TGGGGGCAAG
GTTATGGATC GCCCTGGAAA TTATGTAGAA CCGACAATTG TGACAGGTCT TGGCCACGAT
GCGTCCATTG CACACACAGA GACTTTTGCT CCGATTCTCT ATGTCTTTAA ATTCAAGAAT
GAAGAAGAGG TCTTTGCATG GAATAATGAA GTAAAACAGG GACTTTCAAG TAGCATCTTT
ACCAAAGATC TGGGCAGAAT CTTTCGCTGG CTTGGACCTA AAGGATCAGA CTGTGGCATT
GTAAATGTCA ACATTCCAAC AAGTGGGGCT GAGATTGGAG GTGCCTTTGG AGGAGAAAAG
CACACTGGTG GTGGCAGGGA GTCTGGCAGT GATGCCTGGA AACAGTACAT GAGAAGGTCT
ACTTGTACTA TCAACTACAG TAAAGACCTT CCTCTGGCCC AAGGAATCAA GTTTCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu22668
Clone ID Related Accession (Same CDS sequence) NM_001201377.1
Accession Version NM_001201377.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1536bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-08-28
Organism Homo sapiens(Human)
Product alpha-aminoadipic semialdehyde dehydrogenase isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC375277.1, BC071712.1, AK021800.1, AC093535.2, DB501575.1, BU153725.1, CD678674.1, BG680326.1, AK123774.1 and AI039375.1. On or before Jan 20, 2011 this sequence version replaced XM_003120470.1, XM_003120475.1, XM_003120471.1, XM_003120474.1, XM_003120477.1, XM_003120473.1, XM_003120476.1, XM_003120472.1, XM_003120478.1. Transcript Variant: This variant (1) encodes two isoforms resulting from the use of alternative in-frame translation initiation codons. The longer isoform (1) is derived from an upstream AUG (at nt 193-195), while the shorter isoform (2) is derived from a downstream AUG (at nt 277-279). This RefSeq represents the shorter isoform, which is found in the cytosol (PMIDs: 20207735 and 19885858). Sequence Note: This Refseq, containing three potential in-frame translation initiation codons (all with weak Kozak signals), is annotated with a CDS starting from a downstream start codon (at nt 277-279), which results in a shorter, soluble isoform that is localized in the cytosol (PMIDs: 20207735 and 19885858). A longer isoform, resulting from the use of an upstream start codon (at nt 193-195) and localized in the mitochondria, is represented in a separate RefSeq (NM_001182.4). The use of another upstream start codon (at nt 112-114) that is present in only a subset of higher mammals, would increase the protein length by another 27 aa. This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071712.1, S74728.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:19885858) gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCACTC TCCTCATCAA TCAGCCCCAG TATGCGTGGC TGAAAGAGCT GGGGCTCCGC 
GAGGAAAACG AGGGCGTGTA TAATGGAAGC TGGGGAGGCC GGGGAGAGGT TATTACGACC
TATTGCCCTG CTAACAACGA GCCAATAGCA AGAGTCCGAC AGGCCAGTGT GGCAGACTAT
GAAGAAACTG TAAAGAAAGC AAGAGAAGCA TGGAAAATCT GGGCAGATAT TCCTGCTCCA
AAACGAGGAG AAATAGTAAG ACAGATTGGC GATGCCTTGC GGGAGAAGAT CCAAGTACTA
GGAAGCTTGG TGTCTTTGGA GATGGGGAAA ATCTTAGTGG AAGGTGTGGG TGAAGTTCAG
GAGTATGTGG ATATCTGTGA CTATGCTGTT GGTTTATCAA GGATGATTGG AGGACCTATC
TTGCCTTCTG AAAGATCTGG CCATGCACTG ATTGAGCAGT GGAATCCCGT AGGCCTGGTT
GGAATCATCA CGGCATTCAA TTTCCCTGTG GCAGTGTATG GTTGGAACAA CGCCATCGCC
ATGATCTGTG GAAATGTCTG CCTCTGGAAA GGAGCTCCAA CCACTTCCCT CATTAGTGTG
GCTGTCACAA AGATAATAGC CAAGGTTCTG GAGGACAACA AGCTGCCTGG TGCAATTTGT
TCCTTGACTT GTGGTGGAGC AGATATTGGC ACAGCAATGG CCAAAGATGA ACGAGTGAAC
CTGCTGTCCT TCACTGGGAG CACTCAGGTG GGAAAACAGG TGGGCCTGAT GGTGCAGGAG
AGGTTTGGGA GAAGTCTGTT GGAACTTGGA GGAAACAATG CCATTATTGC CTTTGAAGAT
GCAGACCTCA GCTTAGTTGT TCCATCAGCT CTCTTCGCTG CTGTGGGAAC AGCTGGCCAG
AGGTGTACCA CTGCGAGGCG ACTGTTTATA CATGAAAGCA TCCATGATGA GGTTGTAAAC
AGACTTAAAA AGGCCTATGC ACAGATCCGA GTTGGGAACC CATGGGACCC TAATGTTCTC
TATGGGCCAC TCCACACCAA GCAGGCAGTG AGCATGTTTC TTGGAGCAGT GGAAGAAGCA
AAGAAAGAAG GTGGCACAGT GGTCTATGGG GGCAAGGTTA TGGATCGCCC TGGAAATTAT
GTAGAACCGA CAATTGTGAC AGGTCTTGGC CACGATGCGT CCATTGCACA CACAGAGACT
TTTGCTCCGA TTCTCTATGT CTTTAAATTC AAGAATGAAG AAGAGGTCTT TGCATGGAAT
AATGAAGTAA AACAGGGACT TTCAAGTAGC ATCTTTACCA AAGATCTGGG CAGAATCTTT
CGCTGGCTTG GACCTAAAGG ATCAGACTGT GGCATTGTAA ATGTCAACAT TCCAACAAGT
GGGGCTGAGA TTGGAGGTGC CTTTGGAGGA GAAAAGCACA CTGGTGGTGG CAGGGAGTCT
GGCAGTGATG CCTGGAAACA GTACATGAGA AGGTCTACTT GTACTATCAA CTACAGTAAA
GACCTTCCTC TGGCCCAAGG AATCAAGTTT CAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001188306.1
CDS277..1812
Misc Feature(1)112..114
Misc Feature(2)193..195
Misc Feature(3)349..1773
Misc Feature(4)order(490..492,508..510,517..519,535..537,556..558,673..678,682..684,688..690,694..696,700..711,730..735,739..741,1030..1032,1039..1044,1051..1053,1066..1071,1150..1152,1258..1260,1294..1296,1516..1518,1585..1587,1603..1608,1618..1620,1627..1629,1633..1644,1660..1665,1675..1677,1687..1689,1708..1710,1720..1725,1732..1734,1744..1761,1765..1770)
Misc Feature(5)order(766..777,844..846,850..855,964..969,1006..1017,1024..1026,1033..1035,1078..1086,1180..1182,1471..1473,1678..1680)
Misc Feature(6)order(775..777,1078..1080,1171..1173,1180..1182)
Exon (1)1..384
Exon (2)385..438
Exon (3)439..504
Exon (4)505..585
Exon (5)586..709
Exon (6)710..842
Exon (7)843..887
Exon (8)888..965
Exon (9)966..1063
Exon (10)1064..1105
Exon (11)1106..1200
Exon (12)1201..1285
Exon (13)1286..1392
Exon (14)1393..1509
Exon (15)1510..1607
Exon (16)1608..1681
Exon (17)1682..1757
Exon (18)1758..4936
Translation

Target ORF information:

RefSeq Version NM_001201377.1
Organism Homo sapiens(Human)
Definition Homo sapiens aldehyde dehydrogenase 7 family member A1 (ALDH7A1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001201377.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGTCCACTC TCCTCATCAA TCAGCCCCAG TATGCGTGGC TGAAAGAGCT GGGGCTCCGC 
GAGGAAAACG AGGGCGTGTA TAATGGAAGC TGGGGAGGCC GGGGAGAGGT TATTACGACC
TATTGCCCTG CTAACAACGA GCCAATAGCA AGAGTCCGAC AGGCCAGTGT GGCAGACTAT
GAAGAAACTG TAAAGAAAGC AAGAGAAGCA TGGAAAATCT GGGCAGATAT TCCTGCTCCA
AAACGAGGAG AAATAGTAAG ACAGATTGGC GATGCCTTGC GGGAGAAGAT CCAAGTACTA
GGAAGCTTGG TGTCTTTGGA GATGGGGAAA ATCTTAGTGG AAGGTGTGGG TGAAGTTCAG
GAGTATGTGG ATATCTGTGA CTATGCTGTT GGTTTATCAA GGATGATTGG AGGACCTATC
TTGCCTTCTG AAAGATCTGG CCATGCACTG ATTGAGCAGT GGAATCCCGT AGGCCTGGTT
GGAATCATCA CGGCATTCAA TTTCCCTGTG GCAGTGTATG GTTGGAACAA CGCCATCGCC
ATGATCTGTG GAAATGTCTG CCTCTGGAAA GGAGCTCCAA CCACTTCCCT CATTAGTGTG
GCTGTCACAA AGATAATAGC CAAGGTTCTG GAGGACAACA AGCTGCCTGG TGCAATTTGT
TCCTTGACTT GTGGTGGAGC AGATATTGGC ACAGCAATGG CCAAAGATGA ACGAGTGAAC
CTGCTGTCCT TCACTGGGAG CACTCAGGTG GGAAAACAGG TGGGCCTGAT GGTGCAGGAG
AGGTTTGGGA GAAGTCTGTT GGAACTTGGA GGAAACAATG CCATTATTGC CTTTGAAGAT
GCAGACCTCA GCTTAGTTGT TCCATCAGCT CTCTTCGCTG CTGTGGGAAC AGCTGGCCAG
AGGTGTACCA CTGCGAGGCG ACTGTTTATA CATGAAAGCA TCCATGATGA GGTTGTAAAC
AGACTTAAAA AGGCCTATGC ACAGATCCGA GTTGGGAACC CATGGGACCC TAATGTTCTC
TATGGGCCAC TCCACACCAA GCAGGCAGTG AGCATGTTTC TTGGAGCAGT GGAAGAAGCA
AAGAAAGAAG GTGGCACAGT GGTCTATGGG GGCAAGGTTA TGGATCGCCC TGGAAATTAT
GTAGAACCGA CAATTGTGAC AGGTCTTGGC CACGATGCGT CCATTGCACA CACAGAGACT
TTTGCTCCGA TTCTCTATGT CTTTAAATTC AAGAATGAAG AAGAGGTCTT TGCATGGAAT
AATGAAGTAA AACAGGGACT TTCAAGTAGC ATCTTTACCA AAGATCTGGG CAGAATCTTT
CGCTGGCTTG GACCTAAAGG ATCAGACTGT GGCATTGTAA ATGTCAACAT TCCAACAAGT
GGGGCTGAGA TTGGAGGTGC CTTTGGAGGA GAAAAGCACA CTGGTGGTGG CAGGGAGTCT
GGCAGTGATG CCTGGAAACA GTACATGAGA AGGTCTACTT GTACTATCAA CTACAGTAAA
GACCTTCCTC TGGCCCAAGG AATCAAGTTT CAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56423
Clone ID Related Accession (Same CDS sequence) XM_017009493.1 , XM_011543417.2
Accession Version XM_017009493.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1215bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product alpha-aminoadipic semialdehyde dehydrogenase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGGGAAAA TCTTAGTGGA AGGTGTGGGT GAAGTTCAGG AGTATGTGGA TATCTGTGAC 
TATGCTGTTG GTTTATCAAG GATGATTGGA GGACCTATCT TGCCTTCTGA AAGATCTGGC
CATGCACTGA TTGAGCAGTG GAATCCCGTA GGCCTGGTTG GAATCATCAC GGCATTCAAT
TTCCCTGTGG CAGTGTATGG TTGGAACAAC GCCATCGCCA TGATCTGTGG AAATGTCTGC
CTCTGGAAAG GAGCTCCAAC CACTTCCCTC ATTAGTGTGG CTGTCACAAA GATAATAGCC
AAGGTTCTGG AGGACAACAA GCTGCCTGGT GCAATTTGTT CCTTGACTTG TGGTGGAGCA
GATATTGGCA CAGCAATGGC CAAAGATGAA CGAGTGAACC TGCTGTCCTT CACTGGGAGC
ACTCAGGTGG GAAAACAGGT GGGCCTGATG GTGCAGGAGA GGTTTGGGAG AAGTCTGTTG
GAACTTGGAG GAAACAATGC CATTATTGCC TTTGAAGATG CAGACCTCAG CTTAGTTGTT
CCATCAGCTC TCTTCGCTGC TGTGGGAACA GCTGGCCAGA GGTGTACCAC TGCGAGGCGA
CTGTTTATAC ATGAAAGCAT CCATGATGAG GTTGTAAACA GACTTAAAAA GGCCTATGCA
CAGATCCGAG TTGGGAACCC ATGGGACCCT AATGTTCTCT ATGGGCCACT CCACACCAAG
CAGGCAGTGA GCATGTTTCT TGGAGCAGTG GAAGAAGCAA AGAAAGAAGG TGGCACAGTG
GTCTATGGGG GCAAGGTTAT GGATCGCCCT GGAAATTATG TAGAACCGAC AATTGTGACA
GGTCTTGGCC ACGATGCGTC CATTGCACAC ACAGAGACTT TTGCTCCGAT TCTCTATGTC
TTTAAATTCA AGAATGAAGA AGAGGTCTTT GCATGGAATA ATGAAGTAAA ACAGGGACTT
TCAAGTAGCA TCTTTACCAA AGATCTGGGC AGAATCTTTC GCTGGCTTGG ACCTAAAGGA
TCAGACTGTG GCATTGTAAA TGTCAACATT CCAACAAGTG GGGCTGAGAT TGGAGGTGCC
TTTGGAGGAG AAAAGCACAC TGGTGGTGGC AGGGAGTCTG GCAGTGATGC CTGGAAACAG
TACATGAGAA GGTCTACTTG TACTATCAAC TACAGTAAAG ACCTTCCTCT GGCCCAAGGA
ATCAAGTTTC AGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016864982.1
CDS374..1588
Misc Feature(1)374..1549
Misc Feature(2)order(449..454,458..460,464..466,470..472,476..487,506..511,515..517,806..808,815..820,827..829,842..847,926..928,1034..1036,1070..1072,1292..1294,1361..1363,1379..1384,1394..1396,1403..1405,1409..1420,1436..1441,1451..1453,1463..1465,1484..1486,1496..1501,1508..1510,1520..1537,1541..1546)
Misc Feature(3)order(542..553,620..622,626..631,740..745,782..793,800..802,809..811,854..862,956..958,1247..1249,1454..1456)
Misc Feature(4)order(551..553,854..856,947..949,956..958)
Translation

Target ORF information:

RefSeq Version XM_017009493.1
Organism Homo sapiens(Human)
Definition Homo sapiens aldehyde dehydrogenase 7 family member A1 (ALDH7A1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017009493.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGGGAAAA TCTTAGTGGA AGGTGTGGGT GAAGTTCAGG AGTATGTGGA TATCTGTGAC 
TATGCTGTTG GTTTATCAAG GATGATTGGA GGACCTATCT TGCCTTCTGA AAGATCTGGC
CATGCACTGA TTGAGCAGTG GAATCCCGTA GGCCTGGTTG GAATCATCAC GGCATTCAAT
TTCCCTGTGG CAGTGTATGG TTGGAACAAC GCCATCGCCA TGATCTGTGG AAATGTCTGC
CTCTGGAAAG GAGCTCCAAC CACTTCCCTC ATTAGTGTGG CTGTCACAAA GATAATAGCC
AAGGTTCTGG AGGACAACAA GCTGCCTGGT GCAATTTGTT CCTTGACTTG TGGTGGAGCA
GATATTGGCA CAGCAATGGC CAAAGATGAA CGAGTGAACC TGCTGTCCTT CACTGGGAGC
ACTCAGGTGG GAAAACAGGT GGGCCTGATG GTGCAGGAGA GGTTTGGGAG AAGTCTGTTG
GAACTTGGAG GAAACAATGC CATTATTGCC TTTGAAGATG CAGACCTCAG CTTAGTTGTT
CCATCAGCTC TCTTCGCTGC TGTGGGAACA GCTGGCCAGA GGTGTACCAC TGCGAGGCGA
CTGTTTATAC ATGAAAGCAT CCATGATGAG GTTGTAAACA GACTTAAAAA GGCCTATGCA
CAGATCCGAG TTGGGAACCC ATGGGACCCT AATGTTCTCT ATGGGCCACT CCACACCAAG
CAGGCAGTGA GCATGTTTCT TGGAGCAGTG GAAGAAGCAA AGAAAGAAGG TGGCACAGTG
GTCTATGGGG GCAAGGTTAT GGATCGCCCT GGAAATTATG TAGAACCGAC AATTGTGACA
GGTCTTGGCC ACGATGCGTC CATTGCACAC ACAGAGACTT TTGCTCCGAT TCTCTATGTC
TTTAAATTCA AGAATGAAGA AGAGGTCTTT GCATGGAATA ATGAAGTAAA ACAGGGACTT
TCAAGTAGCA TCTTTACCAA AGATCTGGGC AGAATCTTTC GCTGGCTTGG ACCTAAAGGA
TCAGACTGTG GCATTGTAAA TGTCAACATT CCAACAAGTG GGGCTGAGAT TGGAGGTGCC
TTTGGAGGAG AAAAGCACAC TGGTGGTGGC AGGGAGTCTG GCAGTGATGC CTGGAAACAG
TACATGAGAA GGTCTACTTG TACTATCAAC TACAGTAAAG ACCTTCCTCT GGCCCAAGGA
ATCAAGTTTC AGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56423
Clone ID Related Accession (Same CDS sequence) XM_017009493.1 , XM_011543417.2
Accession Version XM_011543417.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1215bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product alpha-aminoadipic semialdehyde dehydrogenase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543417.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGGGAAAA TCTTAGTGGA AGGTGTGGGT GAAGTTCAGG AGTATGTGGA TATCTGTGAC 
TATGCTGTTG GTTTATCAAG GATGATTGGA GGACCTATCT TGCCTTCTGA AAGATCTGGC
CATGCACTGA TTGAGCAGTG GAATCCCGTA GGCCTGGTTG GAATCATCAC GGCATTCAAT
TTCCCTGTGG CAGTGTATGG TTGGAACAAC GCCATCGCCA TGATCTGTGG AAATGTCTGC
CTCTGGAAAG GAGCTCCAAC CACTTCCCTC ATTAGTGTGG CTGTCACAAA GATAATAGCC
AAGGTTCTGG AGGACAACAA GCTGCCTGGT GCAATTTGTT CCTTGACTTG TGGTGGAGCA
GATATTGGCA CAGCAATGGC CAAAGATGAA CGAGTGAACC TGCTGTCCTT CACTGGGAGC
ACTCAGGTGG GAAAACAGGT GGGCCTGATG GTGCAGGAGA GGTTTGGGAG AAGTCTGTTG
GAACTTGGAG GAAACAATGC CATTATTGCC TTTGAAGATG CAGACCTCAG CTTAGTTGTT
CCATCAGCTC TCTTCGCTGC TGTGGGAACA GCTGGCCAGA GGTGTACCAC TGCGAGGCGA
CTGTTTATAC ATGAAAGCAT CCATGATGAG GTTGTAAACA GACTTAAAAA GGCCTATGCA
CAGATCCGAG TTGGGAACCC ATGGGACCCT AATGTTCTCT ATGGGCCACT CCACACCAAG
CAGGCAGTGA GCATGTTTCT TGGAGCAGTG GAAGAAGCAA AGAAAGAAGG TGGCACAGTG
GTCTATGGGG GCAAGGTTAT GGATCGCCCT GGAAATTATG TAGAACCGAC AATTGTGACA
GGTCTTGGCC ACGATGCGTC CATTGCACAC ACAGAGACTT TTGCTCCGAT TCTCTATGTC
TTTAAATTCA AGAATGAAGA AGAGGTCTTT GCATGGAATA ATGAAGTAAA ACAGGGACTT
TCAAGTAGCA TCTTTACCAA AGATCTGGGC AGAATCTTTC GCTGGCTTGG ACCTAAAGGA
TCAGACTGTG GCATTGTAAA TGTCAACATT CCAACAAGTG GGGCTGAGAT TGGAGGTGCC
TTTGGAGGAG AAAAGCACAC TGGTGGTGGC AGGGAGTCTG GCAGTGATGC CTGGAAACAG
TACATGAGAA GGTCTACTTG TACTATCAAC TACAGTAAAG ACCTTCCTCT GGCCCAAGGA
ATCAAGTTTC AGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011541719.1
CDS589..1803
Misc Feature(1)589..1764
Misc Feature(2)order(664..669,673..675,679..681,685..687,691..702,721..726,730..732,1021..1023,1030..1035,1042..1044,1057..1062,1141..1143,1249..1251,1285..1287,1507..1509,1576..1578,1594..1599,1609..1611,1618..1620,1624..1635,1651..1656,1666..1668,1678..1680,1699..1701,1711..1716,1723..1725,1735..1752,1756..1761)
Misc Feature(3)order(757..768,835..837,841..846,955..960,997..1008,1015..1017,1024..1026,1069..1077,1171..1173,1462..1464,1669..1671)
Misc Feature(4)order(766..768,1069..1071,1162..1164,1171..1173)
Translation

Target ORF information:

RefSeq Version XM_011543417.2
Organism Homo sapiens(Human)
Definition Homo sapiens aldehyde dehydrogenase 7 family member A1 (ALDH7A1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011543417.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGGGAAAA TCTTAGTGGA AGGTGTGGGT GAAGTTCAGG AGTATGTGGA TATCTGTGAC 
TATGCTGTTG GTTTATCAAG GATGATTGGA GGACCTATCT TGCCTTCTGA AAGATCTGGC
CATGCACTGA TTGAGCAGTG GAATCCCGTA GGCCTGGTTG GAATCATCAC GGCATTCAAT
TTCCCTGTGG CAGTGTATGG TTGGAACAAC GCCATCGCCA TGATCTGTGG AAATGTCTGC
CTCTGGAAAG GAGCTCCAAC CACTTCCCTC ATTAGTGTGG CTGTCACAAA GATAATAGCC
AAGGTTCTGG AGGACAACAA GCTGCCTGGT GCAATTTGTT CCTTGACTTG TGGTGGAGCA
GATATTGGCA CAGCAATGGC CAAAGATGAA CGAGTGAACC TGCTGTCCTT CACTGGGAGC
ACTCAGGTGG GAAAACAGGT GGGCCTGATG GTGCAGGAGA GGTTTGGGAG AAGTCTGTTG
GAACTTGGAG GAAACAATGC CATTATTGCC TTTGAAGATG CAGACCTCAG CTTAGTTGTT
CCATCAGCTC TCTTCGCTGC TGTGGGAACA GCTGGCCAGA GGTGTACCAC TGCGAGGCGA
CTGTTTATAC ATGAAAGCAT CCATGATGAG GTTGTAAACA GACTTAAAAA GGCCTATGCA
CAGATCCGAG TTGGGAACCC ATGGGACCCT AATGTTCTCT ATGGGCCACT CCACACCAAG
CAGGCAGTGA GCATGTTTCT TGGAGCAGTG GAAGAAGCAA AGAAAGAAGG TGGCACAGTG
GTCTATGGGG GCAAGGTTAT GGATCGCCCT GGAAATTATG TAGAACCGAC AATTGTGACA
GGTCTTGGCC ACGATGCGTC CATTGCACAC ACAGAGACTT TTGCTCCGAT TCTCTATGTC
TTTAAATTCA AGAATGAAGA AGAGGTCTTT GCATGGAATA ATGAAGTAAA ACAGGGACTT
TCAAGTAGCA TCTTTACCAA AGATCTGGGC AGAATCTTTC GCTGGCTTGG ACCTAAAGGA
TCAGACTGTG GCATTGTAAA TGTCAACATT CCAACAAGTG GGGCTGAGAT TGGAGGTGCC
TTTGGAGGAG AAAAGCACAC TGGTGGTGGC AGGGAGTCTG GCAGTGATGC CTGGAAACAG
TACATGAGAA GGTCTACTTG TACTATCAAC TACAGTAAAG ACCTTCCTCT GGCCCAAGGA
ATCAAGTTTC AGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu100433
Clone ID Related Accession (Same CDS sequence) NM_001202404.1
Accession Version NM_001202404.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-09-11
Organism Homo sapiens(Human)
Product alpha-aminoadipic semialdehyde dehydrogenase isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC375277.1, BC071712.1, AK297365.1, AK021800.1, AC093535.2, DB501575.1, BU153725.1, CD678674.1, BG680326.1, AK123774.1 and AI039375.1. Transcript Variant: This variant (2) is missing two in-frame coding exons compared to variant 1, resulting in a shorter isoform (3) lacking an internal protein segment compared to isoform 1. Sequence Note: This Refseq, containing three potential in-frame translation initiation codons (all with weak Kozak signals), is annotated with a CDS starting from a downstream start codon (at nt 193-195) based on better conservation, N-terminal consistency with homologous proteins, and the presence of a transit peptide, which is essential for the localization of this isoform in the mitochondria (PMIDs: 20207735 and 19885858), and is consistent with the function of this gene in lysine catabolism (which is known to occur in the mitochondria). The use of an upstream start codon (at nt 112-114) that is present in only a subset of higher mammals, would increase the protein length by 27 aa. This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297365.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal (PMID:19885858) gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGTGGCGCC TTCCTCGCGC GCTGTGTGTG CACGCTGCAA AGACCAGCAA GCTCTCTGGA 
CCTTGGAGCA GGCCTGCCGC CTTCATGTCC ACTCTCCTCA TCAATCAGCC CCAGTATGCG
TGGCTGAAAG AGCTGGGGCT CCGCGAGGAA AACGAGGGCG TGTATAATGG AAGCTGGGGA
GGCCGGGGAG AGGTTATTAC GACCTATTGC CCTGCTAACA ACGAGCCAAT AGCAAGAGTC
CGACAGGCCA GTGTGGCAGA CTATGAAGAA ACTGTAAAGA AAGCAAGAGA AGCATGGAAA
ATCTGGGCAG ATATTCCTGC TCCAAAACGA GGAGAAATAG TAAGACAGAT TGGCGATGCC
TTGCGGGAGA AGATCCAAGT ACTAGGAAGC TTGGTGTCTT TGGAGATGGG GAAAATCTTA
GTGGAAGGTG TGGGTGAAGT TCAGGAGTAT GTGGATATCT GTGACTATGC TGTTGGTTTA
TCAAGGATGA TTGGAGGACC TATCTTGCCT TCTGAAAGAT CTGGCCATGC ACTGATTGAG
CAGTGGAATC CCGTAGGCCT GGTTGGAATC ATCACGGCAT TCAATTTCCC TGTGGCAGTG
TATGGTTGGA ACAACGCCAT CGCCATGATC TGTGGAAATG TCTGCCTCTG GAAAGGAGCT
CCAACCACTT CCCTCATTAG TGTGGCTGTC ACAAAGATAA TAGCCAAGGT TCTGGAGGAC
AACAAGCTGC CTGGTGCAAT TTGTTCCTTG ACTTGTGGTG GAGCAGATAT TGGCACAGCA
ATGGCCAAAG ATGAACGAGT GAACCTGCTG TCCTTCACTG GGAGCACTCA GGTGGGAAAA
CAGGTGGGCC TGATGGTGCA GGAGAGGTTT GGGAGAAGTC TGTTGGAACT TGGAGGAAAC
AATGCCATTA TTGCCTTTGA AGATGCAGAC CTCAGCTTAG TTGTTCCATC AGCTCTCTTC
GCTGCTGTGG GAACAGCTGG CCAGAGGTGT ACCACTGCGA GGCGACTGGT TATGGATCGC
CCTGGAAATT ATGTAGAACC GACAATTGTG ACAGGTCTTG GCCACGATGC GTCCATTGCA
CACACAGAGA CTTTTGCTCC GATTCTCTAT GTCTTTAAAT TCAAGAATGA AGAAGAGGTC
TTTGCATGGA ATAATGAAGT AAAACAGGGA CTTTCAAGTA GCATCTTTAC CAAAGATCTG
GGCAGAATCT TTCGCTGGCT TGGACCTAAA GGATCAGACT GTGGCATTGT AAATGTCAAC
ATTCCAACAA GTGGGGCTGA GATTGGAGGT GCCTTTGGAG GAGAAAAGCA CACTGGTGGT
GGCAGGGAGT CTGGCAGTGA TGCCTGGAAA CAGTACATGA GAAGGTCTAC TTGTACTATC
AACTACAGTA AAGACCTTCC TCTGGCCCAA GGAATCAAGT TTCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001189333.2
CDS193..1620
Misc Feature(1)349..1581
Misc Feature(2)order(490..492,508..510,517..519,535..537,556..558,673..678,682..684,688..690,694..696,700..711,730..735,739..741,1030..1032,1039..1044,1051..1053,1066..1071,1150..1152,1324..1326,1393..1395,1411..1416,1426..1428,1435..1437,1441..1452,1468..1473,1483..1485,1495..1497,1516..1518,1528..1533,1540..1542,1552..1569,1573..1578)
Misc Feature(3)order(766..777,844..846,850..855,964..969,1006..1017,1024..1026,1033..1035,1078..1086,1180..1182,1279..1281,1486..1488)
Misc Feature(4)order(775..777,1078..1080,1171..1173,1180..1182)
Exon (1)1..384
Exon (2)385..438
Exon (3)439..504
Exon (4)505..585
Exon (5)586..709
Exon (6)710..842
Exon (7)843..887
Exon (8)888..965
Exon (9)966..1063
Exon (10)1064..1105
Exon (11)1106..1200
Exon (12)1201..1317
Exon (13)1318..1415
Exon (14)1416..1489
Exon (15)1490..1565
Exon (16)1566..4744
Translation

Target ORF information:

RefSeq Version NM_001202404.1
Organism Homo sapiens(Human)
Definition Homo sapiens aldehyde dehydrogenase 7 family member A1 (ALDH7A1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001202404.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGTGGCGCC TTCCTCGCGC GCTGTGTGTG CACGCTGCAA AGACCAGCAA GCTCTCTGGA 
CCTTGGAGCA GGCCTGCCGC CTTCATGTCC ACTCTCCTCA TCAATCAGCC CCAGTATGCG
TGGCTGAAAG AGCTGGGGCT CCGCGAGGAA AACGAGGGCG TGTATAATGG AAGCTGGGGA
GGCCGGGGAG AGGTTATTAC GACCTATTGC CCTGCTAACA ACGAGCCAAT AGCAAGAGTC
CGACAGGCCA GTGTGGCAGA CTATGAAGAA ACTGTAAAGA AAGCAAGAGA AGCATGGAAA
ATCTGGGCAG ATATTCCTGC TCCAAAACGA GGAGAAATAG TAAGACAGAT TGGCGATGCC
TTGCGGGAGA AGATCCAAGT ACTAGGAAGC TTGGTGTCTT TGGAGATGGG GAAAATCTTA
GTGGAAGGTG TGGGTGAAGT TCAGGAGTAT GTGGATATCT GTGACTATGC TGTTGGTTTA
TCAAGGATGA TTGGAGGACC TATCTTGCCT TCTGAAAGAT CTGGCCATGC ACTGATTGAG
CAGTGGAATC CCGTAGGCCT GGTTGGAATC ATCACGGCAT TCAATTTCCC TGTGGCAGTG
TATGGTTGGA ACAACGCCAT CGCCATGATC TGTGGAAATG TCTGCCTCTG GAAAGGAGCT
CCAACCACTT CCCTCATTAG TGTGGCTGTC ACAAAGATAA TAGCCAAGGT TCTGGAGGAC
AACAAGCTGC CTGGTGCAAT TTGTTCCTTG ACTTGTGGTG GAGCAGATAT TGGCACAGCA
ATGGCCAAAG ATGAACGAGT GAACCTGCTG TCCTTCACTG GGAGCACTCA GGTGGGAAAA
CAGGTGGGCC TGATGGTGCA GGAGAGGTTT GGGAGAAGTC TGTTGGAACT TGGAGGAAAC
AATGCCATTA TTGCCTTTGA AGATGCAGAC CTCAGCTTAG TTGTTCCATC AGCTCTCTTC
GCTGCTGTGG GAACAGCTGG CCAGAGGTGT ACCACTGCGA GGCGACTGGT TATGGATCGC
CCTGGAAATT ATGTAGAACC GACAATTGTG ACAGGTCTTG GCCACGATGC GTCCATTGCA
CACACAGAGA CTTTTGCTCC GATTCTCTAT GTCTTTAAAT TCAAGAATGA AGAAGAGGTC
TTTGCATGGA ATAATGAAGT AAAACAGGGA CTTTCAAGTA GCATCTTTAC CAAAGATCTG
GGCAGAATCT TTCGCTGGCT TGGACCTAAA GGATCAGACT GTGGCATTGT AAATGTCAAC
ATTCCAACAA GTGGGGCTGA GATTGGAGGT GCCTTTGGAG GAGAAAAGCA CACTGGTGGT
GGCAGGGAGT CTGGCAGTGA TGCCTGGAAA CAGTACATGA GAAGGTCTAC TTGTACTATC
AACTACAGTA AAGACCTTCC TCTGGCCCAA GGAATCAAGT TTCAGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Intragenic deletions of ALDH7A1 in pyridoxine-dependent epilepsy caused by Alu-Alu recombination.
Neurology85(9)756-62(2015 Sep)
Mefford HC,Zemel M,Geraghty E,Cook J,Clayton PT,Paul K,Plecko B,Mills PB,Nordli DR Jr,Gospe SM Jr


book

Structural Basis of Substrate Recognition by Aldehyde Dehydrogenase 7A1.
Biochemistry54(35)5513-22(2015 Sep)
Luo M,Tanner JJ


book

The ALDH7A1 genetic polymorphisms contribute to development of esophageal squamous cell carcinoma.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine35(12)12665-70(2014 Dec)
Wang H,Tong L,Wei J,Pan W,Li L,Ge Y,Zhou L,Yuan Q,Zhou C,Yang M


book

Clinical diagnosis, treatment, and ALDH7A1 mutations in pyridoxine-dependent epilepsy in three Chinese infants.
PloS one9(3)e92803(2014)
Yang Z,Yang X,Wu Y,Wang J,Zhang Y,Xiong H,Jiang Y,Qin J


 
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