PAPPA cDNA ORF clone, Homo sapiens(human)

The following PAPPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAPPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu20096 NM_002581.5
Latest version!
Homo sapiens pappalysin 1 (PAPPA), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu43320 XM_006717129.3
Latest version!
Homo sapiens pappalysin 1 (PAPPA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu98680 XM_017014784.2
Latest version!
Homo sapiens pappalysin 1 (PAPPA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
View Old Accession Versions >>
OHu20096 NM_002581.3 Homo sapiens pregnancy-associated plasma protein A, pappalysin 1 (PAPPA), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu20096 NM_002581.4 Homo sapiens pappalysin 1 (PAPPA), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu43320 XM_006717129.1 Homo sapiens pregnancy-associated plasma protein A, pappalysin 1 (PAPPA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu43320 XM_006717129.2 Homo sapiens pappalysin 1 (PAPPA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu98680 XM_017014784.1 Homo sapiens pappalysin 1 (PAPPA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $811.30
$1159.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu20096
    Clone ID Related Accession (Same CDS sequence) NM_002581.4 , NM_002581.3 , NM_002581.5
    Accession Version NM_002581.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4884bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-23
    Organism Homo sapiens(human)
    Product pappalysin-1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY795841.2, BC078657.1, X68280.1, AK300129.1, AU134599.1, AL137024.27 and AY623010.1. On Dec 19, 2015 this sequence version replaced NM_002581.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078657.1, SRR1660803.213527.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCGGCTCT GGAGTTGGGT GCTGCACCTG GGGCTGCTGA GCGCCGCGCT GGGCTGCGGG 
    CTGGCCGAGC GTCCCCGCCG GGCCCGGAGA GACCCGCGGG CCGGCCGACC CCCGCGCCCC
    GCCGCCGGCC CGGCCACCTG CGCCACCCGG GCGGCCCGCG GCCGCCGCGC CTCGCCGCCG
    CCGCCGCCGC CGCCGGGCGG TGCCTGGGAA GCCGTGCGCG TCCCCCGGCG GCGGCAGCAG
    CGGGAGGCGA GGGGCGCCAC CGAGGAGCCG AGCCCGCCGA GCCGGGCGCT CTATTTCAGC
    GGGCGAGGCG AGCAGCTGCG CCTCCGGGCC GACCTCGAGC TGCCCCGGGA CGCGTTCACG
    CTGCAAGTGT GGCTGCGAGC GGAGGGGGGC CAGAGGTCTC CGGCAGTGAT CACAGGGCTG
    TATGACAAAT GTTCTTATAT CTCACGTGAC CGAGGATGGG TCGTGGGCAT TCACACCATC
    AGTGACCAAG ACAACAAAGA CCCACGCTAC TTTTTCTCCT TGAAGACAGA CCGAGCCCGG
    CAAGTGACCA CCATCAATGC CCACCGCAGC TACCTCCCAG GCCAGTGGGT ATACCTAGCT
    GCCACCTATG ATGGGCAGTT CATGAAGCTC TATGTGAATG GTGCCCAGGT GGCCACCTCT
    GGGGAACAAG TGGGTGGCAT ATTCAGCCCA CTGACCCAGA AGTGCAAAGT GCTCATGTTA
    GGGGGCAGTG CCCTGAATCA CAACTACCGG GGCTACATCG AGCACTTCAG TCTGTGGAAG
    GTGGCCAGGA CTCAGCGGGA GATACTGTCT GACATGGAAA CCCATGGCGC CCACACTGCT
    CTACCTCAGC TCCTCCTCCA GGAGAACTGG GACAATGTGA AGCATGCCTG GTCCCCCATG
    AAGGATGGCA GCAGCCCCAA AGTGGAATTC AGCAATGCCC ACGGCTTTCT GCTGGACACG
    AGTCTGGAGC CTCCTCTGTG CGGACAGACA TTGTGTGACA ACACAGAGGT CATTGCCAGC
    TACAATCAGC TCTCAAGTTT CCGCCAGCCC AAGGTGGTGC GCTACCGCGT GGTCAACCTC
    TATGAAGATG ATCATAAGAA CCCGACGGTG ACGCGCGAGC AGGTGGACTT CCAGCACCAT
    CAGCTGGCTG AGGCCTTCAA GCAATACAAC ATCTCCTGGG AGCTGGACGT GCTGGAGGTG
    AGCAACTCCT CCCTTCGCCG CCGCCTCATC CTGGCCAACT GTGACATCAG CAAGATTGGG
    GATGAGAACT GTGACCCCGA GTGCAACCAC ACGCTGACGG GCCACGACGG CGGGGATTGC
    CGCCACCTGC GCCACCCTGC CTTCGTGAAG AAGCAGCACA ACGGGGTGTG TGACATGGAC
    TGCAACTATG AACGGTTCAA CTTTGATGGT GGAGAGTGCT GTGACCCTGA AATCACCAAT
    GTCACTCAGA CTTGCTTTGA CCCCGACTCT CCACACAGAG CCTACTTGGA TGTTAATGAG
    CTGAAGAACA TTCTTAAATT GGATGGATCA ACACATCTCA ATATTTTCTT TGCAAAATCC
    TCAGAGGAGG AGTTGGCAGG AGTAGCAACT TGGCCATGGG ACAAGGAGGC CCTGATGCAC
    TTAGGTGGCA TTGTCTTGAA CCCATCTTTC TATGGCATGC CTGGGCACAC CCACACCATG
    ATCCATGAGA TTGGTCACAG CCTGGGCCTC TATCACGTCT TCCGAGGCAT CTCAGAAATC
    CAGTCCTGCA GTGACCCCTG CATGGAGACA GAGCCCTCCT TCGAGACTGG AGACCTCTGC
    AATGATACCA ACCCAGCCCC TAAACACAAG TCCTGTGGTG ACCCAGGGCC AGGAAATGAC
    ACCTGTGGCT TTCATAGCTT CTTCAACACT CCTTACAACA ACTTCATGAG CTATGCAGAT
    GACGACTGTA CGGACTCCTT CACGCCCAAT CAAGTCGCCA GAATGCACTG TTACCTGGAC
    CTGGTCTACC AGGGCTGGCA GCCCTCCAGG AAACCAGCGC CTGTTGCCCT CGCCCCCCAA
    GTTCTGGGCC ACACAACGGA CTCTGTGACA CTGGAGTGGT TCCCACCTAT AGATGGCCAT
    TTCTTTGAAA GAGAATTGGG ATCAGCATGT CATCTTTGCC TGGAAGGGAG AATCCTGGTG
    CAGTATGCTT CCAACGCTTC CTCCCCAATG CCCTGCAGCC CATCAGGACA CTGGAGCCCT
    CGTGAAGCAG AAGGTCATCC TGATGTTGAA CAGCCCTGTA AGTCCAGTGT CCGCACCTGG
    AGCCCAAATT CAGCTGTCAA CCCACACACG GTTCCTCCAG CCTGCCCTGA GCCTCAAGGC
    TGCTACCTCG AGCTGGAGTT CCTCTACCCC TTGGTCCCTG AGTCTCTGAC CATTTGGGTG
    ACCTTTGTCT CCACTGACTG GGACTCTAGT GGAGCTGTCA ATGACATCAA ACTGTTGGCT
    GTCAGTGGGA AGAACATCTC CCTGGGTCCT CAGAATGTCT TCTGTGATGT CCCACTGACC
    ATCAGACTCT GGGACGTGGG CGAGGAGGTG TATGGCATCC AAATCTACAC GCTGGATGAG
    CACCTGGAGA TCGATGCTGC CATGTTGACC TCCACTGCAG ACACCCCACT CTGTCTACAG
    TGTAAGCCCC TGAAGTATAA GGTGGTCCGG GACCCTCCTC TCCAGATGGA TGTGGCCTCC
    ATCCTACATC TCAATAGGAA ATTCGTAGAC ATGGATCTAA ATCTTGGCAG TGTGTACCAG
    TATTGGGTCA TAACTATTTC AGGAACTGAA GAGAGTGAGC CATCACCTGC TGTCACATAC
    ATCCATGGAA GTGGGTACTG TGGCGATGGC ATTATACAAA AAGACCAAGG TGAACAATGC
    GACGACATGA ATAAGATCAA TGGTGATGGC TGCTCCCTTT TCTGCCGACA AGAAGTCTCC
    TTCAATTGTA TTGATGAACC CAGCCGGTGC TATTTCCATG ATGGTGATGG GGTATGTGAG
    GAGTTTGAAC AAAAAACCAG CATTAAGGAC TGTGGTGTCT ACACGCCCCA GGGATTCCTG
    GATCAGTGGG CATCCAATGC TTCAGTATCT CATCAAGACC AGCAATGCCC AGGCTGGGTC
    ATCATCGGAC AGCCAGCAGC ATCCCAGGTG TGTCGAACCA AGGTGATAGA TCTCAGTGAA
    GGCATTTCCC AGCATGCCTG GTACCCTTGC ACCATCAGCT ACCCATATTC CCAGCTGGCT
    CAGACCACTT TTTGGCTCCG GGCGTATTTT TCTCAACCAA TGGTTGCCGC AGCTGTCATT
    GTCCACCTGG TGACGGATGG GACATATTAT GGGGACCAAA AGCAGGAGAC CATCAGCGTG
    CAGCTGCTTG ATACCAAAGA TCAGAGCCAC GATCTAGGCC TCCATGTCCT GAGCTGCAGG
    AACAATCCCC TGATTATCCC TGTGGTCCAT GACCTCAGCC AGCCCTTCTA CCACAGCCAG
    GCGGTACGTG TGAGCTTCAG TTCGCCCCTG GTCGCCATCT CGGGGGTGGC CCTCCGTTCC
    TTCGACAACT TTGACCCCGT CACCCTGAGC AGCTGCCAGA GAGGGGAGAC CTACAGCCCT
    GCCGAGCAGA GCTGCGTGCA CTTCGCATGT GAGAAAACTG ACTGTCCAGA GCTGGCTGTG
    GAGAATGCTT CTCTCAATTG CTCCAGCAGC GACCGCTACC ACGGTGCCCA GTGTACTGTG
    AGCTGCCGGA CAGGCTACGT GCTCCAGATA CGGCGGGATG ATGAGCTGAT CAAGAGCCAG
    ACGGGACCCA GCGTCACAGT GACCTGTACA GAGGGCAAGT GGAATAAGCA GGTGGCCTGT
    GAGCCAGTCG ACTGCAGCAT CCCAGATCAC CATCAAGTCT ATGCTGCCTC CTTCTCCTGC
    CCTGAGGGCA CCACCTTTGG CAGTCAATGT TCCTTCCAGT GCCGTCACCC TGCACAATTG
    AAAGGCAACA ACAGCCTCCT GACCTGCATG GAGGATGGGC TGTGGTCCTT CCCAGAGGCC
    CTGTGTGAGC TCATGTGCCT CGCTCCACCC CCTGTGCCCA ATGCAGACCT CCAGACCGCC
    CGGTGCCGAG AGAATAAGCA CAAGGTGGGC TCCTTCTGCA AATACAAATG CAAGCCTGGA
    TACCATGTGC CTGGATCCTC TCGGAAGTCA AAGAAACGGG CCTTCAAGAC TCAGTGTACC
    CAGGATGGCA GCTGGCAGGA GGGAGCTTGT GTTCCTGTGA CCTGTGACCC ACCTCCACCA
    AAATTCCATG GGCTCTACCA GTGTACTAAT GGCTTCCAGT TCAACAGTGA GTGTAGGATC
    AAGTGTGAAG ACAGTGATGC CTCCCAGGGA CTTGGGAGCA ATGTCATTCA TTGCCGGAAA
    GATGGCACCT GGAACGGCTC CTTCCATGTC TGCCAGGAGA TGCAAGGCCA GTGCTCGGTT
    CCAAACGAGC TCAACAGCAA CCTCAAACTG CAGTGCCCTG ATGGCTATGC CATAGGGTCG
    GAGTGTGCCA CCTCGTGCCT GGACCACAAC AGCGAGTCCA TCATCCTGCC AATGAACGTG
    ACCGTGCGTG ACATCCCCCA CTGGCTGAAC CCCACACGGG TAGAGAGAGT TGTCTGCACT
    GCTGGTCTCA AGTGGTATCC TCACCCTGCT CTGATTCACT GTGTCAAAGG CTGTGAGCCC
    TTCATGGGAG ACAATTATTG TGATGCCATC AACAACCGAG CCTTTTGCAA CTATGACGGT
    GGGGATTGCT GCACCTCCAC AGTGAAGACC AAAAAGGTCA CCCCATTCCC TATGTCCTGT
    GATCTACAAG GTGACTGTGC TTGTCGGGAC CCCCAGGCCC AAGAACACAG CCGGAAAGAC
    CTCCGGGGAT ACAGCCATGG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002572.2
    CDS382..5265
    Translation

    Target ORF information:

    RefSeq Version NM_002581.4
    Organism Homo sapiens(human)
    Definition Homo sapiens pappalysin 1 (PAPPA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002581.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCGGCTCT GGAGTTGGGT GCTGCACCTG GGGCTGCTGA GCGCCGCGCT GGGCTGCGGG 
    CTGGCCGAGC GTCCCCGCCG GGCCCGGAGA GACCCGCGGG CCGGCCGACC CCCGCGCCCC
    GCCGCCGGCC CGGCCACCTG CGCCACCCGG GCGGCCCGCG GCCGCCGCGC CTCGCCGCCG
    CCGCCGCCGC CGCCGGGCGG TGCCTGGGAA GCCGTGCGCG TCCCCCGGCG GCGGCAGCAG
    CGGGAGGCGA GGGGCGCCAC CGAGGAGCCG AGCCCGCCGA GCCGGGCGCT CTATTTCAGC
    GGGCGAGGCG AGCAGCTGCG CCTCCGGGCC GACCTCGAGC TGCCCCGGGA CGCGTTCACG
    CTGCAAGTGT GGCTGCGAGC GGAGGGGGGC CAGAGGTCTC CGGCAGTGAT CACAGGGCTG
    TATGACAAAT GTTCTTATAT CTCACGTGAC CGAGGATGGG TCGTGGGCAT TCACACCATC
    AGTGACCAAG ACAACAAAGA CCCACGCTAC TTTTTCTCCT TGAAGACAGA CCGAGCCCGG
    CAAGTGACCA CCATCAATGC CCACCGCAGC TACCTCCCAG GCCAGTGGGT ATACCTAGCT
    GCCACCTATG ATGGGCAGTT CATGAAGCTC TATGTGAATG GTGCCCAGGT GGCCACCTCT
    GGGGAACAAG TGGGTGGCAT ATTCAGCCCA CTGACCCAGA AGTGCAAAGT GCTCATGTTA
    GGGGGCAGTG CCCTGAATCA CAACTACCGG GGCTACATCG AGCACTTCAG TCTGTGGAAG
    GTGGCCAGGA CTCAGCGGGA GATACTGTCT GACATGGAAA CCCATGGCGC CCACACTGCT
    CTACCTCAGC TCCTCCTCCA GGAGAACTGG GACAATGTGA AGCATGCCTG GTCCCCCATG
    AAGGATGGCA GCAGCCCCAA AGTGGAATTC AGCAATGCCC ACGGCTTTCT GCTGGACACG
    AGTCTGGAGC CTCCTCTGTG CGGACAGACA TTGTGTGACA ACACAGAGGT CATTGCCAGC
    TACAATCAGC TCTCAAGTTT CCGCCAGCCC AAGGTGGTGC GCTACCGCGT GGTCAACCTC
    TATGAAGATG ATCATAAGAA CCCGACGGTG ACGCGCGAGC AGGTGGACTT CCAGCACCAT
    CAGCTGGCTG AGGCCTTCAA GCAATACAAC ATCTCCTGGG AGCTGGACGT GCTGGAGGTG
    AGCAACTCCT CCCTTCGCCG CCGCCTCATC CTGGCCAACT GTGACATCAG CAAGATTGGG
    GATGAGAACT GTGACCCCGA GTGCAACCAC ACGCTGACGG GCCACGACGG CGGGGATTGC
    CGCCACCTGC GCCACCCTGC CTTCGTGAAG AAGCAGCACA ACGGGGTGTG TGACATGGAC
    TGCAACTATG AACGGTTCAA CTTTGATGGT GGAGAGTGCT GTGACCCTGA AATCACCAAT
    GTCACTCAGA CTTGCTTTGA CCCCGACTCT CCACACAGAG CCTACTTGGA TGTTAATGAG
    CTGAAGAACA TTCTTAAATT GGATGGATCA ACACATCTCA ATATTTTCTT TGCAAAATCC
    TCAGAGGAGG AGTTGGCAGG AGTAGCAACT TGGCCATGGG ACAAGGAGGC CCTGATGCAC
    TTAGGTGGCA TTGTCTTGAA CCCATCTTTC TATGGCATGC CTGGGCACAC CCACACCATG
    ATCCATGAGA TTGGTCACAG CCTGGGCCTC TATCACGTCT TCCGAGGCAT CTCAGAAATC
    CAGTCCTGCA GTGACCCCTG CATGGAGACA GAGCCCTCCT TCGAGACTGG AGACCTCTGC
    AATGATACCA ACCCAGCCCC TAAACACAAG TCCTGTGGTG ACCCAGGGCC AGGAAATGAC
    ACCTGTGGCT TTCATAGCTT CTTCAACACT CCTTACAACA ACTTCATGAG CTATGCAGAT
    GACGACTGTA CGGACTCCTT CACGCCCAAT CAAGTCGCCA GAATGCACTG TTACCTGGAC
    CTGGTCTACC AGGGCTGGCA GCCCTCCAGG AAACCAGCGC CTGTTGCCCT CGCCCCCCAA
    GTTCTGGGCC ACACAACGGA CTCTGTGACA CTGGAGTGGT TCCCACCTAT AGATGGCCAT
    TTCTTTGAAA GAGAATTGGG ATCAGCATGT CATCTTTGCC TGGAAGGGAG AATCCTGGTG
    CAGTATGCTT CCAACGCTTC CTCCCCAATG CCCTGCAGCC CATCAGGACA CTGGAGCCCT
    CGTGAAGCAG AAGGTCATCC TGATGTTGAA CAGCCCTGTA AGTCCAGTGT CCGCACCTGG
    AGCCCAAATT CAGCTGTCAA CCCACACACG GTTCCTCCAG CCTGCCCTGA GCCTCAAGGC
    TGCTACCTCG AGCTGGAGTT CCTCTACCCC TTGGTCCCTG AGTCTCTGAC CATTTGGGTG
    ACCTTTGTCT CCACTGACTG GGACTCTAGT GGAGCTGTCA ATGACATCAA ACTGTTGGCT
    GTCAGTGGGA AGAACATCTC CCTGGGTCCT CAGAATGTCT TCTGTGATGT CCCACTGACC
    ATCAGACTCT GGGACGTGGG CGAGGAGGTG TATGGCATCC AAATCTACAC GCTGGATGAG
    CACCTGGAGA TCGATGCTGC CATGTTGACC TCCACTGCAG ACACCCCACT CTGTCTACAG
    TGTAAGCCCC TGAAGTATAA GGTGGTCCGG GACCCTCCTC TCCAGATGGA TGTGGCCTCC
    ATCCTACATC TCAATAGGAA ATTCGTAGAC ATGGATCTAA ATCTTGGCAG TGTGTACCAG
    TATTGGGTCA TAACTATTTC AGGAACTGAA GAGAGTGAGC CATCACCTGC TGTCACATAC
    ATCCATGGAA GTGGGTACTG TGGCGATGGC ATTATACAAA AAGACCAAGG TGAACAATGC
    GACGACATGA ATAAGATCAA TGGTGATGGC TGCTCCCTTT TCTGCCGACA AGAAGTCTCC
    TTCAATTGTA TTGATGAACC CAGCCGGTGC TATTTCCATG ATGGTGATGG GGTATGTGAG
    GAGTTTGAAC AAAAAACCAG CATTAAGGAC TGTGGTGTCT ACACGCCCCA GGGATTCCTG
    GATCAGTGGG CATCCAATGC TTCAGTATCT CATCAAGACC AGCAATGCCC AGGCTGGGTC
    ATCATCGGAC AGCCAGCAGC ATCCCAGGTG TGTCGAACCA AGGTGATAGA TCTCAGTGAA
    GGCATTTCCC AGCATGCCTG GTACCCTTGC ACCATCAGCT ACCCATATTC CCAGCTGGCT
    CAGACCACTT TTTGGCTCCG GGCGTATTTT TCTCAACCAA TGGTTGCCGC AGCTGTCATT
    GTCCACCTGG TGACGGATGG GACATATTAT GGGGACCAAA AGCAGGAGAC CATCAGCGTG
    CAGCTGCTTG ATACCAAAGA TCAGAGCCAC GATCTAGGCC TCCATGTCCT GAGCTGCAGG
    AACAATCCCC TGATTATCCC TGTGGTCCAT GACCTCAGCC AGCCCTTCTA CCACAGCCAG
    GCGGTACGTG TGAGCTTCAG TTCGCCCCTG GTCGCCATCT CGGGGGTGGC CCTCCGTTCC
    TTCGACAACT TTGACCCCGT CACCCTGAGC AGCTGCCAGA GAGGGGAGAC CTACAGCCCT
    GCCGAGCAGA GCTGCGTGCA CTTCGCATGT GAGAAAACTG ACTGTCCAGA GCTGGCTGTG
    GAGAATGCTT CTCTCAATTG CTCCAGCAGC GACCGCTACC ACGGTGCCCA GTGTACTGTG
    AGCTGCCGGA CAGGCTACGT GCTCCAGATA CGGCGGGATG ATGAGCTGAT CAAGAGCCAG
    ACGGGACCCA GCGTCACAGT GACCTGTACA GAGGGCAAGT GGAATAAGCA GGTGGCCTGT
    GAGCCAGTCG ACTGCAGCAT CCCAGATCAC CATCAAGTCT ATGCTGCCTC CTTCTCCTGC
    CCTGAGGGCA CCACCTTTGG CAGTCAATGT TCCTTCCAGT GCCGTCACCC TGCACAATTG
    AAAGGCAACA ACAGCCTCCT GACCTGCATG GAGGATGGGC TGTGGTCCTT CCCAGAGGCC
    CTGTGTGAGC TCATGTGCCT CGCTCCACCC CCTGTGCCCA ATGCAGACCT CCAGACCGCC
    CGGTGCCGAG AGAATAAGCA CAAGGTGGGC TCCTTCTGCA AATACAAATG CAAGCCTGGA
    TACCATGTGC CTGGATCCTC TCGGAAGTCA AAGAAACGGG CCTTCAAGAC TCAGTGTACC
    CAGGATGGCA GCTGGCAGGA GGGAGCTTGT GTTCCTGTGA CCTGTGACCC ACCTCCACCA
    AAATTCCATG GGCTCTACCA GTGTACTAAT GGCTTCCAGT TCAACAGTGA GTGTAGGATC
    AAGTGTGAAG ACAGTGATGC CTCCCAGGGA CTTGGGAGCA ATGTCATTCA TTGCCGGAAA
    GATGGCACCT GGAACGGCTC CTTCCATGTC TGCCAGGAGA TGCAAGGCCA GTGCTCGGTT
    CCAAACGAGC TCAACAGCAA CCTCAAACTG CAGTGCCCTG ATGGCTATGC CATAGGGTCG
    GAGTGTGCCA CCTCGTGCCT GGACCACAAC AGCGAGTCCA TCATCCTGCC AATGAACGTG
    ACCGTGCGTG ACATCCCCCA CTGGCTGAAC CCCACACGGG TAGAGAGAGT TGTCTGCACT
    GCTGGTCTCA AGTGGTATCC TCACCCTGCT CTGATTCACT GTGTCAAAGG CTGTGAGCCC
    TTCATGGGAG ACAATTATTG TGATGCCATC AACAACCGAG CCTTTTGCAA CTATGACGGT
    GGGGATTGCT GCACCTCCAC AGTGAAGACC AAAAAGGTCA CCCCATTCCC TATGTCCTGT
    GATCTACAAG GTGACTGTGC TTGTCGGGAC CCCCAGGCCC AAGAACACAG CCGGAAAGAC
    CTCCGGGGAT ACAGCCATGG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20096
    Clone ID Related Accession (Same CDS sequence) NM_002581.4 , NM_002581.3 , NM_002581.5
    Accession Version NM_002581.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4884bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product pappalysin-1 preproprotein
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCGGCTCT GGAGTTGGGT GCTGCACCTG GGGCTGCTGA GCGCCGCGCT GGGCTGCGGG 
    CTGGCCGAGC GTCCCCGCCG GGCCCGGAGA GACCCGCGGG CCGGCCGACC CCCGCGCCCC
    GCCGCCGGCC CGGCCACCTG CGCCACCCGG GCGGCCCGCG GCCGCCGCGC CTCGCCGCCG
    CCGCCGCCGC CGCCGGGCGG TGCCTGGGAA GCCGTGCGCG TCCCCCGGCG GCGGCAGCAG
    CGGGAGGCGA GGGGCGCCAC CGAGGAGCCG AGCCCGCCGA GCCGGGCGCT CTATTTCAGC
    GGGCGAGGCG AGCAGCTGCG CCTCCGGGCC GACCTCGAGC TGCCCCGGGA CGCGTTCACG
    CTGCAAGTGT GGCTGCGAGC GGAGGGGGGC CAGAGGTCTC CGGCAGTGAT CACAGGGCTG
    TATGACAAAT GTTCTTATAT CTCACGTGAC CGAGGATGGG TCGTGGGCAT TCACACCATC
    AGTGACCAAG ACAACAAAGA CCCACGCTAC TTTTTCTCCT TGAAGACAGA CCGAGCCCGG
    CAAGTGACCA CCATCAATGC CCACCGCAGC TACCTCCCAG GCCAGTGGGT ATACCTAGCT
    GCCACCTATG ATGGGCAGTT CATGAAGCTC TATGTGAATG GTGCCCAGGT GGCCACCTCT
    GGGGAACAAG TGGGTGGCAT ATTCAGCCCA CTGACCCAGA AGTGCAAAGT GCTCATGTTA
    GGGGGCAGTG CCCTGAATCA CAACTACCGG GGCTACATCG AGCACTTCAG TCTGTGGAAG
    GTGGCCAGGA CTCAGCGGGA GATACTGTCT GACATGGAAA CCCATGGCGC CCACACTGCT
    CTACCTCAGC TCCTCCTCCA GGAGAACTGG GACAATGTGA AGCATGCCTG GTCCCCCATG
    AAGGATGGCA GCAGCCCCAA AGTGGAATTC AGCAATGCCC ACGGCTTTCT GCTGGACACG
    AGTCTGGAGC CTCCTCTGTG CGGACAGACA TTGTGTGACA ACACAGAGGT CATTGCCAGC
    TACAATCAGC TCTCAAGTTT CCGCCAGCCC AAGGTGGTGC GCTACCGCGT GGTCAACCTC
    TATGAAGATG ATCATAAGAA CCCGACGGTG ACGCGCGAGC AGGTGGACTT CCAGCACCAT
    CAGCTGGCTG AGGCCTTCAA GCAATACAAC ATCTCCTGGG AGCTGGACGT GCTGGAGGTG
    AGCAACTCCT CCCTTCGCCG CCGCCTCATC CTGGCCAACT GTGACATCAG CAAGATTGGG
    GATGAGAACT GTGACCCCGA GTGCAACCAC ACGCTGACGG GCCACGACGG CGGGGATTGC
    CGCCACCTGC GCCACCCTGC CTTCGTGAAG AAGCAGCACA ACGGGGTGTG TGACATGGAC
    TGCAACTATG AACGGTTCAA CTTTGATGGT GGAGAGTGCT GTGACCCTGA AATCACCAAT
    GTCACTCAGA CTTGCTTTGA CCCCGACTCT CCACACAGAG CCTACTTGGA TGTTAATGAG
    CTGAAGAACA TTCTTAAATT GGATGGATCA ACACATCTCA ATATTTTCTT TGCAAAATCC
    TCAGAGGAGG AGTTGGCAGG AGTAGCAACT TGGCCATGGG ACAAGGAGGC CCTGATGCAC
    TTAGGTGGCA TTGTCTTGAA CCCATCTTTC TATGGCATGC CTGGGCACAC CCACACCATG
    ATCCATGAGA TTGGTCACAG CCTGGGCCTC TATCACGTCT TCCGAGGCAT CTCAGAAATC
    CAGTCCTGCA GTGACCCCTG CATGGAGACA GAGCCCTCCT TCGAGACTGG AGACCTCTGC
    AATGATACCA ACCCAGCCCC TAAACACAAG TCCTGTGGTG ACCCAGGGCC AGGAAATGAC
    ACCTGTGGCT TTCATAGCTT CTTCAACACT CCTTACAACA ACTTCATGAG CTATGCAGAT
    GACGACTGTA CGGACTCCTT CACGCCCAAT CAAGTCGCCA GAATGCACTG TTACCTGGAC
    CTGGTCTACC AGGGCTGGCA GCCCTCCAGG AAACCAGCGC CTGTTGCCCT CGCCCCCCAA
    GTTCTGGGCC ACACAACGGA CTCTGTGACA CTGGAGTGGT TCCCACCTAT AGATGGCCAT
    TTCTTTGAAA GAGAATTGGG ATCAGCATGT CATCTTTGCC TGGAAGGGAG AATCCTGGTG
    CAGTATGCTT CCAACGCTTC CTCCCCAATG CCCTGCAGCC CATCAGGACA CTGGAGCCCT
    CGTGAAGCAG AAGGTCATCC TGATGTTGAA CAGCCCTGTA AGTCCAGTGT CCGCACCTGG
    AGCCCAAATT CAGCTGTCAA CCCACACACG GTTCCTCCAG CCTGCCCTGA GCCTCAAGGC
    TGCTACCTCG AGCTGGAGTT CCTCTACCCC TTGGTCCCTG AGTCTCTGAC CATTTGGGTG
    ACCTTTGTCT CCACTGACTG GGACTCTAGT GGAGCTGTCA ATGACATCAA ACTGTTGGCT
    GTCAGTGGGA AGAACATCTC CCTGGGTCCT CAGAATGTCT TCTGTGATGT CCCACTGACC
    ATCAGACTCT GGGACGTGGG CGAGGAGGTG TATGGCATCC AAATCTACAC GCTGGATGAG
    CACCTGGAGA TCGATGCTGC CATGTTGACC TCCACTGCAG ACACCCCACT CTGTCTACAG
    TGTAAGCCCC TGAAGTATAA GGTGGTCCGG GACCCTCCTC TCCAGATGGA TGTGGCCTCC
    ATCCTACATC TCAATAGGAA ATTCGTAGAC ATGGATCTAA ATCTTGGCAG TGTGTACCAG
    TATTGGGTCA TAACTATTTC AGGAACTGAA GAGAGTGAGC CATCACCTGC TGTCACATAC
    ATCCATGGAA GTGGGTACTG TGGCGATGGC ATTATACAAA AAGACCAAGG TGAACAATGC
    GACGACATGA ATAAGATCAA TGGTGATGGC TGCTCCCTTT TCTGCCGACA AGAAGTCTCC
    TTCAATTGTA TTGATGAACC CAGCCGGTGC TATTTCCATG ATGGTGATGG GGTATGTGAG
    GAGTTTGAAC AAAAAACCAG CATTAAGGAC TGTGGTGTCT ACACGCCCCA GGGATTCCTG
    GATCAGTGGG CATCCAATGC TTCAGTATCT CATCAAGACC AGCAATGCCC AGGCTGGGTC
    ATCATCGGAC AGCCAGCAGC ATCCCAGGTG TGTCGAACCA AGGTGATAGA TCTCAGTGAA
    GGCATTTCCC AGCATGCCTG GTACCCTTGC ACCATCAGCT ACCCATATTC CCAGCTGGCT
    CAGACCACTT TTTGGCTCCG GGCGTATTTT TCTCAACCAA TGGTTGCCGC AGCTGTCATT
    GTCCACCTGG TGACGGATGG GACATATTAT GGGGACCAAA AGCAGGAGAC CATCAGCGTG
    CAGCTGCTTG ATACCAAAGA TCAGAGCCAC GATCTAGGCC TCCATGTCCT GAGCTGCAGG
    AACAATCCCC TGATTATCCC TGTGGTCCAT GACCTCAGCC AGCCCTTCTA CCACAGCCAG
    GCGGTACGTG TGAGCTTCAG TTCGCCCCTG GTCGCCATCT CGGGGGTGGC CCTCCGTTCC
    TTCGACAACT TTGACCCCGT CACCCTGAGC AGCTGCCAGA GAGGGGAGAC CTACAGCCCT
    GCCGAGCAGA GCTGCGTGCA CTTCGCATGT GAGAAAACTG ACTGTCCAGA GCTGGCTGTG
    GAGAATGCTT CTCTCAATTG CTCCAGCAGC GACCGCTACC ACGGTGCCCA GTGTACTGTG
    AGCTGCCGGA CAGGCTACGT GCTCCAGATA CGGCGGGATG ATGAGCTGAT CAAGAGCCAG
    ACGGGACCCA GCGTCACAGT GACCTGTACA GAGGGCAAGT GGAATAAGCA GGTGGCCTGT
    GAGCCAGTCG ACTGCAGCAT CCCAGATCAC CATCAAGTCT ATGCTGCCTC CTTCTCCTGC
    CCTGAGGGCA CCACCTTTGG CAGTCAATGT TCCTTCCAGT GCCGTCACCC TGCACAATTG
    AAAGGCAACA ACAGCCTCCT GACCTGCATG GAGGATGGGC TGTGGTCCTT CCCAGAGGCC
    CTGTGTGAGC TCATGTGCCT CGCTCCACCC CCTGTGCCCA ATGCAGACCT CCAGACCGCC
    CGGTGCCGAG AGAATAAGCA CAAGGTGGGC TCCTTCTGCA AATACAAATG CAAGCCTGGA
    TACCATGTGC CTGGATCCTC TCGGAAGTCA AAGAAACGGG CCTTCAAGAC TCAGTGTACC
    CAGGATGGCA GCTGGCAGGA GGGAGCTTGT GTTCCTGTGA CCTGTGACCC ACCTCCACCA
    AAATTCCATG GGCTCTACCA GTGTACTAAT GGCTTCCAGT TCAACAGTGA GTGTAGGATC
    AAGTGTGAAG ACAGTGATGC CTCCCAGGGA CTTGGGAGCA ATGTCATTCA TTGCCGGAAA
    GATGGCACCT GGAACGGCTC CTTCCATGTC TGCCAGGAGA TGCAAGGCCA GTGCTCGGTT
    CCAAACGAGC TCAACAGCAA CCTCAAACTG CAGTGCCCTG ATGGCTATGC CATAGGGTCG
    GAGTGTGCCA CCTCGTGCCT GGACCACAAC AGCGAGTCCA TCATCCTGCC AATGAACGTG
    ACCGTGCGTG ACATCCCCCA CTGGCTGAAC CCCACACGGG TAGAGAGAGT TGTCTGCACT
    GCTGGTCTCA AGTGGTATCC TCACCCTGCT CTGATTCACT GTGTCAAAGG CTGTGAGCCC
    TTCATGGGAG ACAATTATTG TGATGCCATC AACAACCGAG CCTTTTGCAA CTATGACGGT
    GGGGATTGCT GCACCTCCAC AGTGAAGACC AAAAAGGTCA CCCCATTCCC TATGTCCTGT
    GATCTACAAG GTGACTGTGC TTGTCGGGAC CCCCAGGCCC AAGAACACAG CCGGAAAGAC
    CTCCGGGGAT ACAGCCATGG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002572.2
    Translation

    Target ORF information:

    RefSeq Version NM_002581.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens pregnancy-associated plasma protein A, pappalysin 1 (PAPPA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002581.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCGGCTCT GGAGTTGGGT GCTGCACCTG GGGCTGCTGA GCGCCGCGCT GGGCTGCGGG 
    CTGGCCGAGC GTCCCCGCCG GGCCCGGAGA GACCCGCGGG CCGGCCGACC CCCGCGCCCC
    GCCGCCGGCC CGGCCACCTG CGCCACCCGG GCGGCCCGCG GCCGCCGCGC CTCGCCGCCG
    CCGCCGCCGC CGCCGGGCGG TGCCTGGGAA GCCGTGCGCG TCCCCCGGCG GCGGCAGCAG
    CGGGAGGCGA GGGGCGCCAC CGAGGAGCCG AGCCCGCCGA GCCGGGCGCT CTATTTCAGC
    GGGCGAGGCG AGCAGCTGCG CCTCCGGGCC GACCTCGAGC TGCCCCGGGA CGCGTTCACG
    CTGCAAGTGT GGCTGCGAGC GGAGGGGGGC CAGAGGTCTC CGGCAGTGAT CACAGGGCTG
    TATGACAAAT GTTCTTATAT CTCACGTGAC CGAGGATGGG TCGTGGGCAT TCACACCATC
    AGTGACCAAG ACAACAAAGA CCCACGCTAC TTTTTCTCCT TGAAGACAGA CCGAGCCCGG
    CAAGTGACCA CCATCAATGC CCACCGCAGC TACCTCCCAG GCCAGTGGGT ATACCTAGCT
    GCCACCTATG ATGGGCAGTT CATGAAGCTC TATGTGAATG GTGCCCAGGT GGCCACCTCT
    GGGGAACAAG TGGGTGGCAT ATTCAGCCCA CTGACCCAGA AGTGCAAAGT GCTCATGTTA
    GGGGGCAGTG CCCTGAATCA CAACTACCGG GGCTACATCG AGCACTTCAG TCTGTGGAAG
    GTGGCCAGGA CTCAGCGGGA GATACTGTCT GACATGGAAA CCCATGGCGC CCACACTGCT
    CTACCTCAGC TCCTCCTCCA GGAGAACTGG GACAATGTGA AGCATGCCTG GTCCCCCATG
    AAGGATGGCA GCAGCCCCAA AGTGGAATTC AGCAATGCCC ACGGCTTTCT GCTGGACACG
    AGTCTGGAGC CTCCTCTGTG CGGACAGACA TTGTGTGACA ACACAGAGGT CATTGCCAGC
    TACAATCAGC TCTCAAGTTT CCGCCAGCCC AAGGTGGTGC GCTACCGCGT GGTCAACCTC
    TATGAAGATG ATCATAAGAA CCCGACGGTG ACGCGCGAGC AGGTGGACTT CCAGCACCAT
    CAGCTGGCTG AGGCCTTCAA GCAATACAAC ATCTCCTGGG AGCTGGACGT GCTGGAGGTG
    AGCAACTCCT CCCTTCGCCG CCGCCTCATC CTGGCCAACT GTGACATCAG CAAGATTGGG
    GATGAGAACT GTGACCCCGA GTGCAACCAC ACGCTGACGG GCCACGACGG CGGGGATTGC
    CGCCACCTGC GCCACCCTGC CTTCGTGAAG AAGCAGCACA ACGGGGTGTG TGACATGGAC
    TGCAACTATG AACGGTTCAA CTTTGATGGT GGAGAGTGCT GTGACCCTGA AATCACCAAT
    GTCACTCAGA CTTGCTTTGA CCCCGACTCT CCACACAGAG CCTACTTGGA TGTTAATGAG
    CTGAAGAACA TTCTTAAATT GGATGGATCA ACACATCTCA ATATTTTCTT TGCAAAATCC
    TCAGAGGAGG AGTTGGCAGG AGTAGCAACT TGGCCATGGG ACAAGGAGGC CCTGATGCAC
    TTAGGTGGCA TTGTCTTGAA CCCATCTTTC TATGGCATGC CTGGGCACAC CCACACCATG
    ATCCATGAGA TTGGTCACAG CCTGGGCCTC TATCACGTCT TCCGAGGCAT CTCAGAAATC
    CAGTCCTGCA GTGACCCCTG CATGGAGACA GAGCCCTCCT TCGAGACTGG AGACCTCTGC
    AATGATACCA ACCCAGCCCC TAAACACAAG TCCTGTGGTG ACCCAGGGCC AGGAAATGAC
    ACCTGTGGCT TTCATAGCTT CTTCAACACT CCTTACAACA ACTTCATGAG CTATGCAGAT
    GACGACTGTA CGGACTCCTT CACGCCCAAT CAAGTCGCCA GAATGCACTG TTACCTGGAC
    CTGGTCTACC AGGGCTGGCA GCCCTCCAGG AAACCAGCGC CTGTTGCCCT CGCCCCCCAA
    GTTCTGGGCC ACACAACGGA CTCTGTGACA CTGGAGTGGT TCCCACCTAT AGATGGCCAT
    TTCTTTGAAA GAGAATTGGG ATCAGCATGT CATCTTTGCC TGGAAGGGAG AATCCTGGTG
    CAGTATGCTT CCAACGCTTC CTCCCCAATG CCCTGCAGCC CATCAGGACA CTGGAGCCCT
    CGTGAAGCAG AAGGTCATCC TGATGTTGAA CAGCCCTGTA AGTCCAGTGT CCGCACCTGG
    AGCCCAAATT CAGCTGTCAA CCCACACACG GTTCCTCCAG CCTGCCCTGA GCCTCAAGGC
    TGCTACCTCG AGCTGGAGTT CCTCTACCCC TTGGTCCCTG AGTCTCTGAC CATTTGGGTG
    ACCTTTGTCT CCACTGACTG GGACTCTAGT GGAGCTGTCA ATGACATCAA ACTGTTGGCT
    GTCAGTGGGA AGAACATCTC CCTGGGTCCT CAGAATGTCT TCTGTGATGT CCCACTGACC
    ATCAGACTCT GGGACGTGGG CGAGGAGGTG TATGGCATCC AAATCTACAC GCTGGATGAG
    CACCTGGAGA TCGATGCTGC CATGTTGACC TCCACTGCAG ACACCCCACT CTGTCTACAG
    TGTAAGCCCC TGAAGTATAA GGTGGTCCGG GACCCTCCTC TCCAGATGGA TGTGGCCTCC
    ATCCTACATC TCAATAGGAA ATTCGTAGAC ATGGATCTAA ATCTTGGCAG TGTGTACCAG
    TATTGGGTCA TAACTATTTC AGGAACTGAA GAGAGTGAGC CATCACCTGC TGTCACATAC
    ATCCATGGAA GTGGGTACTG TGGCGATGGC ATTATACAAA AAGACCAAGG TGAACAATGC
    GACGACATGA ATAAGATCAA TGGTGATGGC TGCTCCCTTT TCTGCCGACA AGAAGTCTCC
    TTCAATTGTA TTGATGAACC CAGCCGGTGC TATTTCCATG ATGGTGATGG GGTATGTGAG
    GAGTTTGAAC AAAAAACCAG CATTAAGGAC TGTGGTGTCT ACACGCCCCA GGGATTCCTG
    GATCAGTGGG CATCCAATGC TTCAGTATCT CATCAAGACC AGCAATGCCC AGGCTGGGTC
    ATCATCGGAC AGCCAGCAGC ATCCCAGGTG TGTCGAACCA AGGTGATAGA TCTCAGTGAA
    GGCATTTCCC AGCATGCCTG GTACCCTTGC ACCATCAGCT ACCCATATTC CCAGCTGGCT
    CAGACCACTT TTTGGCTCCG GGCGTATTTT TCTCAACCAA TGGTTGCCGC AGCTGTCATT
    GTCCACCTGG TGACGGATGG GACATATTAT GGGGACCAAA AGCAGGAGAC CATCAGCGTG
    CAGCTGCTTG ATACCAAAGA TCAGAGCCAC GATCTAGGCC TCCATGTCCT GAGCTGCAGG
    AACAATCCCC TGATTATCCC TGTGGTCCAT GACCTCAGCC AGCCCTTCTA CCACAGCCAG
    GCGGTACGTG TGAGCTTCAG TTCGCCCCTG GTCGCCATCT CGGGGGTGGC CCTCCGTTCC
    TTCGACAACT TTGACCCCGT CACCCTGAGC AGCTGCCAGA GAGGGGAGAC CTACAGCCCT
    GCCGAGCAGA GCTGCGTGCA CTTCGCATGT GAGAAAACTG ACTGTCCAGA GCTGGCTGTG
    GAGAATGCTT CTCTCAATTG CTCCAGCAGC GACCGCTACC ACGGTGCCCA GTGTACTGTG
    AGCTGCCGGA CAGGCTACGT GCTCCAGATA CGGCGGGATG ATGAGCTGAT CAAGAGCCAG
    ACGGGACCCA GCGTCACAGT GACCTGTACA GAGGGCAAGT GGAATAAGCA GGTGGCCTGT
    GAGCCAGTCG ACTGCAGCAT CCCAGATCAC CATCAAGTCT ATGCTGCCTC CTTCTCCTGC
    CCTGAGGGCA CCACCTTTGG CAGTCAATGT TCCTTCCAGT GCCGTCACCC TGCACAATTG
    AAAGGCAACA ACAGCCTCCT GACCTGCATG GAGGATGGGC TGTGGTCCTT CCCAGAGGCC
    CTGTGTGAGC TCATGTGCCT CGCTCCACCC CCTGTGCCCA ATGCAGACCT CCAGACCGCC
    CGGTGCCGAG AGAATAAGCA CAAGGTGGGC TCCTTCTGCA AATACAAATG CAAGCCTGGA
    TACCATGTGC CTGGATCCTC TCGGAAGTCA AAGAAACGGG CCTTCAAGAC TCAGTGTACC
    CAGGATGGCA GCTGGCAGGA GGGAGCTTGT GTTCCTGTGA CCTGTGACCC ACCTCCACCA
    AAATTCCATG GGCTCTACCA GTGTACTAAT GGCTTCCAGT TCAACAGTGA GTGTAGGATC
    AAGTGTGAAG ACAGTGATGC CTCCCAGGGA CTTGGGAGCA ATGTCATTCA TTGCCGGAAA
    GATGGCACCT GGAACGGCTC CTTCCATGTC TGCCAGGAGA TGCAAGGCCA GTGCTCGGTT
    CCAAACGAGC TCAACAGCAA CCTCAAACTG CAGTGCCCTG ATGGCTATGC CATAGGGTCG
    GAGTGTGCCA CCTCGTGCCT GGACCACAAC AGCGAGTCCA TCATCCTGCC AATGAACGTG
    ACCGTGCGTG ACATCCCCCA CTGGCTGAAC CCCACACGGG TAGAGAGAGT TGTCTGCACT
    GCTGGTCTCA AGTGGTATCC TCACCCTGCT CTGATTCACT GTGTCAAAGG CTGTGAGCCC
    TTCATGGGAG ACAATTATTG TGATGCCATC AACAACCGAG CCTTTTGCAA CTATGACGGT
    GGGGATTGCT GCACCTCCAC AGTGAAGACC AAAAAGGTCA CCCCATTCCC TATGTCCTGT
    GATCTACAAG GTGACTGTGC TTGTCGGGAC CCCCAGGCCC AAGAACACAG CCGGAAAGAC
    CTCCGGGGAT ACAGCCATGG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20096
    Clone ID Related Accession (Same CDS sequence) NM_002581.4 , NM_002581.3 , NM_002581.5
    Accession Version NM_002581.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4884bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product pappalysin-1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL691426.9, BY795841.2, BC078657.1, X68280.1, AK300129.1, AU134599.1, AL137024.27 and AY623010.1. On Nov 23, 2018 this sequence version replaced NM_002581.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078657.1, SRR1660803.213527.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000328252.4/ ENSP00000330658.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCGGCTCT GGAGTTGGGT GCTGCACCTG GGGCTGCTGA GCGCCGCGCT GGGCTGCGGG 
    CTGGCCGAGC GTCCCCGCCG GGCCCGGAGA GACCCGCGGG CCGGCCGACC CCCGCGCCCC
    GCCGCCGGCC CGGCCACCTG CGCCACCCGG GCGGCCCGCG GCCGCCGCGC CTCGCCGCCG
    CCGCCGCCGC CGCCGGGCGG TGCCTGGGAA GCCGTGCGCG TCCCCCGGCG GCGGCAGCAG
    CGGGAGGCGA GGGGCGCCAC CGAGGAGCCG AGCCCGCCGA GCCGGGCGCT CTATTTCAGC
    GGGCGAGGCG AGCAGCTGCG CCTCCGGGCC GACCTCGAGC TGCCCCGGGA CGCGTTCACG
    CTGCAAGTGT GGCTGCGAGC GGAGGGGGGC CAGAGGTCTC CGGCAGTGAT CACAGGGCTG
    TATGACAAAT GTTCTTATAT CTCACGTGAC CGAGGATGGG TCGTGGGCAT TCACACCATC
    AGTGACCAAG ACAACAAAGA CCCACGCTAC TTTTTCTCCT TGAAGACAGA CCGAGCCCGG
    CAAGTGACCA CCATCAATGC CCACCGCAGC TACCTCCCAG GCCAGTGGGT ATACCTAGCT
    GCCACCTATG ATGGGCAGTT CATGAAGCTC TATGTGAATG GTGCCCAGGT GGCCACCTCT
    GGGGAACAAG TGGGTGGCAT ATTCAGCCCA CTGACCCAGA AGTGCAAAGT GCTCATGTTA
    GGGGGCAGTG CCCTGAATCA CAACTACCGG GGCTACATCG AGCACTTCAG TCTGTGGAAG
    GTGGCCAGGA CTCAGCGGGA GATACTGTCT GACATGGAAA CCCATGGCGC CCACACTGCT
    CTACCTCAGC TCCTCCTCCA GGAGAACTGG GACAATGTGA AGCATGCCTG GTCCCCCATG
    AAGGATGGCA GCAGCCCCAA AGTGGAATTC AGCAATGCCC ACGGCTTTCT GCTGGACACG
    AGTCTGGAGC CTCCTCTGTG CGGACAGACA TTGTGTGACA ACACAGAGGT CATTGCCAGC
    TACAATCAGC TCTCAAGTTT CCGCCAGCCC AAGGTGGTGC GCTACCGCGT GGTCAACCTC
    TATGAAGATG ATCATAAGAA CCCGACGGTG ACGCGCGAGC AGGTGGACTT CCAGCACCAT
    CAGCTGGCTG AGGCCTTCAA GCAATACAAC ATCTCCTGGG AGCTGGACGT GCTGGAGGTG
    AGCAACTCCT CCCTTCGCCG CCGCCTCATC CTGGCCAACT GTGACATCAG CAAGATTGGG
    GATGAGAACT GTGACCCCGA GTGCAACCAC ACGCTGACGG GCCACGACGG CGGGGATTGC
    CGCCACCTGC GCCACCCTGC CTTCGTGAAG AAGCAGCACA ACGGGGTGTG TGACATGGAC
    TGCAACTATG AACGGTTCAA CTTTGATGGT GGAGAGTGCT GTGACCCTGA AATCACCAAT
    GTCACTCAGA CTTGCTTTGA CCCCGACTCT CCACACAGAG CCTACTTGGA TGTTAATGAG
    CTGAAGAACA TTCTTAAATT GGATGGATCA ACACATCTCA ATATTTTCTT TGCAAAATCC
    TCAGAGGAGG AGTTGGCAGG AGTAGCAACT TGGCCATGGG ACAAGGAGGC CCTGATGCAC
    TTAGGTGGCA TTGTCTTGAA CCCATCTTTC TATGGCATGC CTGGGCACAC CCACACCATG
    ATCCATGAGA TTGGTCACAG CCTGGGCCTC TATCACGTCT TCCGAGGCAT CTCAGAAATC
    CAGTCCTGCA GTGACCCCTG CATGGAGACA GAGCCCTCCT TCGAGACTGG AGACCTCTGC
    AATGATACCA ACCCAGCCCC TAAACACAAG TCCTGTGGTG ACCCAGGGCC AGGAAATGAC
    ACCTGTGGCT TTCATAGCTT CTTCAACACT CCTTACAACA ACTTCATGAG CTATGCAGAT
    GACGACTGTA CGGACTCCTT CACGCCCAAT CAAGTCGCCA GAATGCACTG TTACCTGGAC
    CTGGTCTACC AGGGCTGGCA GCCCTCCAGG AAACCAGCGC CTGTTGCCCT CGCCCCCCAA
    GTTCTGGGCC ACACAACGGA CTCTGTGACA CTGGAGTGGT TCCCACCTAT AGATGGCCAT
    TTCTTTGAAA GAGAATTGGG ATCAGCATGT CATCTTTGCC TGGAAGGGAG AATCCTGGTG
    CAGTATGCTT CCAACGCTTC CTCCCCAATG CCCTGCAGCC CATCAGGACA CTGGAGCCCT
    CGTGAAGCAG AAGGTCATCC TGATGTTGAA CAGCCCTGTA AGTCCAGTGT CCGCACCTGG
    AGCCCAAATT CAGCTGTCAA CCCACACACG GTTCCTCCAG CCTGCCCTGA GCCTCAAGGC
    TGCTACCTCG AGCTGGAGTT CCTCTACCCC TTGGTCCCTG AGTCTCTGAC CATTTGGGTG
    ACCTTTGTCT CCACTGACTG GGACTCTAGT GGAGCTGTCA ATGACATCAA ACTGTTGGCT
    GTCAGTGGGA AGAACATCTC CCTGGGTCCT CAGAATGTCT TCTGTGATGT CCCACTGACC
    ATCAGACTCT GGGACGTGGG CGAGGAGGTG TATGGCATCC AAATCTACAC GCTGGATGAG
    CACCTGGAGA TCGATGCTGC CATGTTGACC TCCACTGCAG ACACCCCACT CTGTCTACAG
    TGTAAGCCCC TGAAGTATAA GGTGGTCCGG GACCCTCCTC TCCAGATGGA TGTGGCCTCC
    ATCCTACATC TCAATAGGAA ATTCGTAGAC ATGGATCTAA ATCTTGGCAG TGTGTACCAG
    TATTGGGTCA TAACTATTTC AGGAACTGAA GAGAGTGAGC CATCACCTGC TGTCACATAC
    ATCCATGGAA GTGGGTACTG TGGCGATGGC ATTATACAAA AAGACCAAGG TGAACAATGC
    GACGACATGA ATAAGATCAA TGGTGATGGC TGCTCCCTTT TCTGCCGACA AGAAGTCTCC
    TTCAATTGTA TTGATGAACC CAGCCGGTGC TATTTCCATG ATGGTGATGG GGTATGTGAG
    GAGTTTGAAC AAAAAACCAG CATTAAGGAC TGTGGTGTCT ACACGCCCCA GGGATTCCTG
    GATCAGTGGG CATCCAATGC TTCAGTATCT CATCAAGACC AGCAATGCCC AGGCTGGGTC
    ATCATCGGAC AGCCAGCAGC ATCCCAGGTG TGTCGAACCA AGGTGATAGA TCTCAGTGAA
    GGCATTTCCC AGCATGCCTG GTACCCTTGC ACCATCAGCT ACCCATATTC CCAGCTGGCT
    CAGACCACTT TTTGGCTCCG GGCGTATTTT TCTCAACCAA TGGTTGCCGC AGCTGTCATT
    GTCCACCTGG TGACGGATGG GACATATTAT GGGGACCAAA AGCAGGAGAC CATCAGCGTG
    CAGCTGCTTG ATACCAAAGA TCAGAGCCAC GATCTAGGCC TCCATGTCCT GAGCTGCAGG
    AACAATCCCC TGATTATCCC TGTGGTCCAT GACCTCAGCC AGCCCTTCTA CCACAGCCAG
    GCGGTACGTG TGAGCTTCAG TTCGCCCCTG GTCGCCATCT CGGGGGTGGC CCTCCGTTCC
    TTCGACAACT TTGACCCCGT CACCCTGAGC AGCTGCCAGA GAGGGGAGAC CTACAGCCCT
    GCCGAGCAGA GCTGCGTGCA CTTCGCATGT GAGAAAACTG ACTGTCCAGA GCTGGCTGTG
    GAGAATGCTT CTCTCAATTG CTCCAGCAGC GACCGCTACC ACGGTGCCCA GTGTACTGTG
    AGCTGCCGGA CAGGCTACGT GCTCCAGATA CGGCGGGATG ATGAGCTGAT CAAGAGCCAG
    ACGGGACCCA GCGTCACAGT GACCTGTACA GAGGGCAAGT GGAATAAGCA GGTGGCCTGT
    GAGCCAGTCG ACTGCAGCAT CCCAGATCAC CATCAAGTCT ATGCTGCCTC CTTCTCCTGC
    CCTGAGGGCA CCACCTTTGG CAGTCAATGT TCCTTCCAGT GCCGTCACCC TGCACAATTG
    AAAGGCAACA ACAGCCTCCT GACCTGCATG GAGGATGGGC TGTGGTCCTT CCCAGAGGCC
    CTGTGTGAGC TCATGTGCCT CGCTCCACCC CCTGTGCCCA ATGCAGACCT CCAGACCGCC
    CGGTGCCGAG AGAATAAGCA CAAGGTGGGC TCCTTCTGCA AATACAAATG CAAGCCTGGA
    TACCATGTGC CTGGATCCTC TCGGAAGTCA AAGAAACGGG CCTTCAAGAC TCAGTGTACC
    CAGGATGGCA GCTGGCAGGA GGGAGCTTGT GTTCCTGTGA CCTGTGACCC ACCTCCACCA
    AAATTCCATG GGCTCTACCA GTGTACTAAT GGCTTCCAGT TCAACAGTGA GTGTAGGATC
    AAGTGTGAAG ACAGTGATGC CTCCCAGGGA CTTGGGAGCA ATGTCATTCA TTGCCGGAAA
    GATGGCACCT GGAACGGCTC CTTCCATGTC TGCCAGGAGA TGCAAGGCCA GTGCTCGGTT
    CCAAACGAGC TCAACAGCAA CCTCAAACTG CAGTGCCCTG ATGGCTATGC CATAGGGTCG
    GAGTGTGCCA CCTCGTGCCT GGACCACAAC AGCGAGTCCA TCATCCTGCC AATGAACGTG
    ACCGTGCGTG ACATCCCCCA CTGGCTGAAC CCCACACGGG TAGAGAGAGT TGTCTGCACT
    GCTGGTCTCA AGTGGTATCC TCACCCTGCT CTGATTCACT GTGTCAAAGG CTGTGAGCCC
    TTCATGGGAG ACAATTATTG TGATGCCATC AACAACCGAG CCTTTTGCAA CTATGACGGT
    GGGGATTGCT GCACCTCCAC AGTGAAGACC AAAAAGGTCA CCCCATTCCC TATGTCCTGT
    GATCTACAAG GTGACTGTGC TTGTCGGGAC CCCCAGGCCC AAGAACACAG CCGGAAAGAC
    CTCCGGGGAT ACAGCCATGG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002572.2
    CDS383..5266
    Translation

    Target ORF information:

    RefSeq Version NM_002581.5
    Organism Homo sapiens(human)
    Definition Homo sapiens pappalysin 1 (PAPPA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002581.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGCGGCTCT GGAGTTGGGT GCTGCACCTG GGGCTGCTGA GCGCCGCGCT GGGCTGCGGG 
    CTGGCCGAGC GTCCCCGCCG GGCCCGGAGA GACCCGCGGG CCGGCCGACC CCCGCGCCCC
    GCCGCCGGCC CGGCCACCTG CGCCACCCGG GCGGCCCGCG GCCGCCGCGC CTCGCCGCCG
    CCGCCGCCGC CGCCGGGCGG TGCCTGGGAA GCCGTGCGCG TCCCCCGGCG GCGGCAGCAG
    CGGGAGGCGA GGGGCGCCAC CGAGGAGCCG AGCCCGCCGA GCCGGGCGCT CTATTTCAGC
    GGGCGAGGCG AGCAGCTGCG CCTCCGGGCC GACCTCGAGC TGCCCCGGGA CGCGTTCACG
    CTGCAAGTGT GGCTGCGAGC GGAGGGGGGC CAGAGGTCTC CGGCAGTGAT CACAGGGCTG
    TATGACAAAT GTTCTTATAT CTCACGTGAC CGAGGATGGG TCGTGGGCAT TCACACCATC
    AGTGACCAAG ACAACAAAGA CCCACGCTAC TTTTTCTCCT TGAAGACAGA CCGAGCCCGG
    CAAGTGACCA CCATCAATGC CCACCGCAGC TACCTCCCAG GCCAGTGGGT ATACCTAGCT
    GCCACCTATG ATGGGCAGTT CATGAAGCTC TATGTGAATG GTGCCCAGGT GGCCACCTCT
    GGGGAACAAG TGGGTGGCAT ATTCAGCCCA CTGACCCAGA AGTGCAAAGT GCTCATGTTA
    GGGGGCAGTG CCCTGAATCA CAACTACCGG GGCTACATCG AGCACTTCAG TCTGTGGAAG
    GTGGCCAGGA CTCAGCGGGA GATACTGTCT GACATGGAAA CCCATGGCGC CCACACTGCT
    CTACCTCAGC TCCTCCTCCA GGAGAACTGG GACAATGTGA AGCATGCCTG GTCCCCCATG
    AAGGATGGCA GCAGCCCCAA AGTGGAATTC AGCAATGCCC ACGGCTTTCT GCTGGACACG
    AGTCTGGAGC CTCCTCTGTG CGGACAGACA TTGTGTGACA ACACAGAGGT CATTGCCAGC
    TACAATCAGC TCTCAAGTTT CCGCCAGCCC AAGGTGGTGC GCTACCGCGT GGTCAACCTC
    TATGAAGATG ATCATAAGAA CCCGACGGTG ACGCGCGAGC AGGTGGACTT CCAGCACCAT
    CAGCTGGCTG AGGCCTTCAA GCAATACAAC ATCTCCTGGG AGCTGGACGT GCTGGAGGTG
    AGCAACTCCT CCCTTCGCCG CCGCCTCATC CTGGCCAACT GTGACATCAG CAAGATTGGG
    GATGAGAACT GTGACCCCGA GTGCAACCAC ACGCTGACGG GCCACGACGG CGGGGATTGC
    CGCCACCTGC GCCACCCTGC CTTCGTGAAG AAGCAGCACA ACGGGGTGTG TGACATGGAC
    TGCAACTATG AACGGTTCAA CTTTGATGGT GGAGAGTGCT GTGACCCTGA AATCACCAAT
    GTCACTCAGA CTTGCTTTGA CCCCGACTCT CCACACAGAG CCTACTTGGA TGTTAATGAG
    CTGAAGAACA TTCTTAAATT GGATGGATCA ACACATCTCA ATATTTTCTT TGCAAAATCC
    TCAGAGGAGG AGTTGGCAGG AGTAGCAACT TGGCCATGGG ACAAGGAGGC CCTGATGCAC
    TTAGGTGGCA TTGTCTTGAA CCCATCTTTC TATGGCATGC CTGGGCACAC CCACACCATG
    ATCCATGAGA TTGGTCACAG CCTGGGCCTC TATCACGTCT TCCGAGGCAT CTCAGAAATC
    CAGTCCTGCA GTGACCCCTG CATGGAGACA GAGCCCTCCT TCGAGACTGG AGACCTCTGC
    AATGATACCA ACCCAGCCCC TAAACACAAG TCCTGTGGTG ACCCAGGGCC AGGAAATGAC
    ACCTGTGGCT TTCATAGCTT CTTCAACACT CCTTACAACA ACTTCATGAG CTATGCAGAT
    GACGACTGTA CGGACTCCTT CACGCCCAAT CAAGTCGCCA GAATGCACTG TTACCTGGAC
    CTGGTCTACC AGGGCTGGCA GCCCTCCAGG AAACCAGCGC CTGTTGCCCT CGCCCCCCAA
    GTTCTGGGCC ACACAACGGA CTCTGTGACA CTGGAGTGGT TCCCACCTAT AGATGGCCAT
    TTCTTTGAAA GAGAATTGGG ATCAGCATGT CATCTTTGCC TGGAAGGGAG AATCCTGGTG
    CAGTATGCTT CCAACGCTTC CTCCCCAATG CCCTGCAGCC CATCAGGACA CTGGAGCCCT
    CGTGAAGCAG AAGGTCATCC TGATGTTGAA CAGCCCTGTA AGTCCAGTGT CCGCACCTGG
    AGCCCAAATT CAGCTGTCAA CCCACACACG GTTCCTCCAG CCTGCCCTGA GCCTCAAGGC
    TGCTACCTCG AGCTGGAGTT CCTCTACCCC TTGGTCCCTG AGTCTCTGAC CATTTGGGTG
    ACCTTTGTCT CCACTGACTG GGACTCTAGT GGAGCTGTCA ATGACATCAA ACTGTTGGCT
    GTCAGTGGGA AGAACATCTC CCTGGGTCCT CAGAATGTCT TCTGTGATGT CCCACTGACC
    ATCAGACTCT GGGACGTGGG CGAGGAGGTG TATGGCATCC AAATCTACAC GCTGGATGAG
    CACCTGGAGA TCGATGCTGC CATGTTGACC TCCACTGCAG ACACCCCACT CTGTCTACAG
    TGTAAGCCCC TGAAGTATAA GGTGGTCCGG GACCCTCCTC TCCAGATGGA TGTGGCCTCC
    ATCCTACATC TCAATAGGAA ATTCGTAGAC ATGGATCTAA ATCTTGGCAG TGTGTACCAG
    TATTGGGTCA TAACTATTTC AGGAACTGAA GAGAGTGAGC CATCACCTGC TGTCACATAC
    ATCCATGGAA GTGGGTACTG TGGCGATGGC ATTATACAAA AAGACCAAGG TGAACAATGC
    GACGACATGA ATAAGATCAA TGGTGATGGC TGCTCCCTTT TCTGCCGACA AGAAGTCTCC
    TTCAATTGTA TTGATGAACC CAGCCGGTGC TATTTCCATG ATGGTGATGG GGTATGTGAG
    GAGTTTGAAC AAAAAACCAG CATTAAGGAC TGTGGTGTCT ACACGCCCCA GGGATTCCTG
    GATCAGTGGG CATCCAATGC TTCAGTATCT CATCAAGACC AGCAATGCCC AGGCTGGGTC
    ATCATCGGAC AGCCAGCAGC ATCCCAGGTG TGTCGAACCA AGGTGATAGA TCTCAGTGAA
    GGCATTTCCC AGCATGCCTG GTACCCTTGC ACCATCAGCT ACCCATATTC CCAGCTGGCT
    CAGACCACTT TTTGGCTCCG GGCGTATTTT TCTCAACCAA TGGTTGCCGC AGCTGTCATT
    GTCCACCTGG TGACGGATGG GACATATTAT GGGGACCAAA AGCAGGAGAC CATCAGCGTG
    CAGCTGCTTG ATACCAAAGA TCAGAGCCAC GATCTAGGCC TCCATGTCCT GAGCTGCAGG
    AACAATCCCC TGATTATCCC TGTGGTCCAT GACCTCAGCC AGCCCTTCTA CCACAGCCAG
    GCGGTACGTG TGAGCTTCAG TTCGCCCCTG GTCGCCATCT CGGGGGTGGC CCTCCGTTCC
    TTCGACAACT TTGACCCCGT CACCCTGAGC AGCTGCCAGA GAGGGGAGAC CTACAGCCCT
    GCCGAGCAGA GCTGCGTGCA CTTCGCATGT GAGAAAACTG ACTGTCCAGA GCTGGCTGTG
    GAGAATGCTT CTCTCAATTG CTCCAGCAGC GACCGCTACC ACGGTGCCCA GTGTACTGTG
    AGCTGCCGGA CAGGCTACGT GCTCCAGATA CGGCGGGATG ATGAGCTGAT CAAGAGCCAG
    ACGGGACCCA GCGTCACAGT GACCTGTACA GAGGGCAAGT GGAATAAGCA GGTGGCCTGT
    GAGCCAGTCG ACTGCAGCAT CCCAGATCAC CATCAAGTCT ATGCTGCCTC CTTCTCCTGC
    CCTGAGGGCA CCACCTTTGG CAGTCAATGT TCCTTCCAGT GCCGTCACCC TGCACAATTG
    AAAGGCAACA ACAGCCTCCT GACCTGCATG GAGGATGGGC TGTGGTCCTT CCCAGAGGCC
    CTGTGTGAGC TCATGTGCCT CGCTCCACCC CCTGTGCCCA ATGCAGACCT CCAGACCGCC
    CGGTGCCGAG AGAATAAGCA CAAGGTGGGC TCCTTCTGCA AATACAAATG CAAGCCTGGA
    TACCATGTGC CTGGATCCTC TCGGAAGTCA AAGAAACGGG CCTTCAAGAC TCAGTGTACC
    CAGGATGGCA GCTGGCAGGA GGGAGCTTGT GTTCCTGTGA CCTGTGACCC ACCTCCACCA
    AAATTCCATG GGCTCTACCA GTGTACTAAT GGCTTCCAGT TCAACAGTGA GTGTAGGATC
    AAGTGTGAAG ACAGTGATGC CTCCCAGGGA CTTGGGAGCA ATGTCATTCA TTGCCGGAAA
    GATGGCACCT GGAACGGCTC CTTCCATGTC TGCCAGGAGA TGCAAGGCCA GTGCTCGGTT
    CCAAACGAGC TCAACAGCAA CCTCAAACTG CAGTGCCCTG ATGGCTATGC CATAGGGTCG
    GAGTGTGCCA CCTCGTGCCT GGACCACAAC AGCGAGTCCA TCATCCTGCC AATGAACGTG
    ACCGTGCGTG ACATCCCCCA CTGGCTGAAC CCCACACGGG TAGAGAGAGT TGTCTGCACT
    GCTGGTCTCA AGTGGTATCC TCACCCTGCT CTGATTCACT GTGTCAAAGG CTGTGAGCCC
    TTCATGGGAG ACAATTATTG TGATGCCATC AACAACCGAG CCTTTTGCAA CTATGACGGT
    GGGGATTGCT GCACCTCCAC AGTGAAGACC AAAAAGGTCA CCCCATTCCC TATGTCCTGT
    GATCTACAAG GTGACTGTGC TTGTCGGGAC CCCCAGGCCC AAGAACACAG CCGGAAAGAC
    CTCCGGGGAT ACAGCCATGG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43320
    Clone ID Related Accession (Same CDS sequence) XM_006717129.3 , XM_006717129.1 , XM_006717129.2
    Accession Version XM_006717129.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pappalysin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006717129.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCATTT CTTTGAAAGA ATTGGGATCA GCATGTCATC TTTGCCTGGA AGGGAGAATC 
    CTGGTGCAGT ATGCTTCCAA CGCTTCCTCC CCAATGCCCT GCAGCCCATC AGGACACTGG
    AGCCCTCGTG AAGCAGAAGG TCATCCTGAT GTTGAACAGC CCTGTAAGTC CAGTGTCCGC
    ACCTGGAGCC CAAATTCAGC TGTCAACCCA CACACGGTTC CTCCAGCCTG CCCTGAGCCT
    CAAGGCTGCT ACCTCGAGCT GGAGTTCCTC TACCCCTTGG TCCCTGAGTC TCTGACCATT
    TGGGTGACCT TTGTCTCCAC TGACTGGGAC TCTAGTGGAG CTGTCAATGA CATCAAACTG
    TTGGCTGTCA GTGGGAAGAA CATCTCCCTG GGTCCTCAGA ATGTCTTCTG TGATGTCCCA
    CTGACCATCA GACTCTGGGA CGTGGGCGAG GAGGTGTATG GCATCCAAAT CTACACGCTG
    GATGAGCACC TGGAGATCGA TGCTGCCATG TTGACCTCCA CTGCAGACAC CCCACTCTGT
    CTACAGTGTA AGCCCCTGAA GTATAAGGTG GTCCGGGACC CTCCTCTCCA GATGGATGTG
    GCCTCCATCC TACATCTCAA TAGGAAATTC GTAGACATGG ATCTAAATCT TGGCAGTGTG
    TACCAGTATT GGGTCATAAC TATTTCAGGA ACTGAAGAGA GTGAGCCATC ACCTGCTGTC
    ACATACATCC ATGGAAGTGG GTACTGTGGC GATGGCATTA TACAAAAAGA CCAAGGTGAA
    CAATGCGACG ACATGAATAA GATCAATGGT GATGGCTGCT CCCTTTTCTG CCGACAAGAA
    GTCTCCTTCA ATTGTATTGA TGAACCCAGC CGGTGCTATT TCCATGATGG TGATGGGGTA
    TGTGAGGAGT TTGAACAAAA AACCAGCATT AAGGACTGTG GTGTCTACAC GCCCCAGGGA
    TTCCTGGATC AGTGGGCATC CAATGCTTCA GTATCTCATC AAGACCAGCA ATGCCCAGGC
    TGGGTCATCA TCGGACAGCC AGCAGCATCC CAGGTGTGTC GAACCAAGGT GATAGATCTC
    AGTGAAGGCA TTTCCCAGCA TGCCTGGTAC CCTTGCACCA TCAGCTACCC ATATTCCCAG
    CTGGCTCAGA CCACTTTTTG GCTCCGGGCG TATTTTTCTC AACCAATGGT TGCCGCAGCT
    GTCATTGTCC ACCTGGTGAC GGATGGGACA TATTATGGGG ACCAAAAGCA GGAGACCATC
    AGCGTGCAGC TGCTTGATAC CAAAGATCAG AGCCACGATC TAGGCCTCCA TGTCCTGAGC
    TGCAGGAACA ATCCCCTGAT TATCCCTGTG GTCCATGACC TCAGCCAGCC CTTCTACCAC
    AGCCAGGCGG TACGTGTGAG CTTCAGTTCG CCCCTGGTCG CCATCTCGGG GGTGGCCCTC
    CGTTCCTTCG ACAACTTTGA CCCCGTCACC CTGAGCAGCT GCCAGAGAGG GGAGACCTAC
    AGCCCTGCCG AGCAGAGCTG CGTGCACTTC GCATGTGAGA AAACTGACTG TCCAGAGCTG
    GCTGTGGAGA ATGCTTCTCT CAATTGCTCC AGCAGCGACC GCTACCACGG TGCCCAGTGT
    ACTGTGAGCT GCCGGACAGG CTACGTGCTC CAGATACGGC GGGATGATGA GCTGATCAAG
    AGCCAGACGG GACCCAGCGT CACAGTGACC TGTACAGAGG GCAAGTGGAA TAAGCAGGTG
    GCCTGTGAGC CAGTCGACTG CAGCATCCCA GATCACCATC AAGTCTATGC TGCCTCCTTC
    TCCTGCCCTG AGGGCACCAC CTTTGGCAGT CAATGTTCCT TCCAGTGCCG TCACCCTGCA
    CAATTGAAAG GCAACAACAG CCTCCTGACC TGCATGGAGG ATGGGCTGTG GTCCTTCCCA
    GAGGCCCTGT GTGAGCTCAT GTGCCTCGCT CCACCCCCTG TGCCCAATGC AGACCTCCAG
    ACCGCCCGGT GCCGAGAGAA TAAGCACAAG GTGGGCTCCT TCTGCAAATA CAAATGCAAG
    CCTGGATACC ATGTGCCTGG ATCCTCTCGG AAGTCAAAGA AACGGGCCTT CAAGACTCAG
    TGTACCCAGG ATGGCAGCTG GCAGGAGGGA GCTTGTGTTC CTGTGACCTG TGACCCACCT
    CCACCAAAAT TCCATGGGCT CTACCAGTGT ACTAATGGCT TCCAGTTCAA CAGTGAGTGT
    AGGATCAAGT GTGAAGACAG TGATGCCTCC CAGGGACTTG GGAGCAATGT CATTCATTGC
    CGGAAAGATG GCACCTGGAA CGGCTCCTTC CATGTCTGCC AGGAGATGCA AGGCCAGTGC
    TCGGTTCCAA ACGAGCTCAA CAGCAACCTC AAACTGCAGT GCCCTGATGG CTATGCCATA
    GGGTCGGAGT GTGCCACCTC GTGCCTGGAC CACAACAGCG AGTCCATCAT CCTGCCAATG
    AACGTGACCG TGCGTGACAT CCCCCACTGG CTGAACCCCA CACGGGTAGA GAGAGTTGTC
    TGCACTGCTG GTCTCAAGTG GTATCCTCAC CCTGCTCTGA TTCACTGTGT CAAAGGCTGT
    GAGCCCTTCA TGGGAGACAA TTATTGTGAT GCCATCAACA ACCGAGCCTT TTGCAACTAT
    GACGGTGGGG ATTGCTGCAC CTCCACAGTG AAGACCAAAA AGGTCACCCC ATTCCCTATG
    TCCTGTGATC TACAAGGTGA CTGTGCTTGT CGGGACCCCC AGGCCCAAGA ACACAGCCGG
    AAAGACCTCC GGGGATACAG CCATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006717192.1
    CDS550..3339
    Translation

    Target ORF information:

    RefSeq Version XM_006717129.3
    Organism Homo sapiens(human)
    Definition Homo sapiens pappalysin 1 (PAPPA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006717129.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCATTT CTTTGAAAGA ATTGGGATCA GCATGTCATC TTTGCCTGGA AGGGAGAATC 
    CTGGTGCAGT ATGCTTCCAA CGCTTCCTCC CCAATGCCCT GCAGCCCATC AGGACACTGG
    AGCCCTCGTG AAGCAGAAGG TCATCCTGAT GTTGAACAGC CCTGTAAGTC CAGTGTCCGC
    ACCTGGAGCC CAAATTCAGC TGTCAACCCA CACACGGTTC CTCCAGCCTG CCCTGAGCCT
    CAAGGCTGCT ACCTCGAGCT GGAGTTCCTC TACCCCTTGG TCCCTGAGTC TCTGACCATT
    TGGGTGACCT TTGTCTCCAC TGACTGGGAC TCTAGTGGAG CTGTCAATGA CATCAAACTG
    TTGGCTGTCA GTGGGAAGAA CATCTCCCTG GGTCCTCAGA ATGTCTTCTG TGATGTCCCA
    CTGACCATCA GACTCTGGGA CGTGGGCGAG GAGGTGTATG GCATCCAAAT CTACACGCTG
    GATGAGCACC TGGAGATCGA TGCTGCCATG TTGACCTCCA CTGCAGACAC CCCACTCTGT
    CTACAGTGTA AGCCCCTGAA GTATAAGGTG GTCCGGGACC CTCCTCTCCA GATGGATGTG
    GCCTCCATCC TACATCTCAA TAGGAAATTC GTAGACATGG ATCTAAATCT TGGCAGTGTG
    TACCAGTATT GGGTCATAAC TATTTCAGGA ACTGAAGAGA GTGAGCCATC ACCTGCTGTC
    ACATACATCC ATGGAAGTGG GTACTGTGGC GATGGCATTA TACAAAAAGA CCAAGGTGAA
    CAATGCGACG ACATGAATAA GATCAATGGT GATGGCTGCT CCCTTTTCTG CCGACAAGAA
    GTCTCCTTCA ATTGTATTGA TGAACCCAGC CGGTGCTATT TCCATGATGG TGATGGGGTA
    TGTGAGGAGT TTGAACAAAA AACCAGCATT AAGGACTGTG GTGTCTACAC GCCCCAGGGA
    TTCCTGGATC AGTGGGCATC CAATGCTTCA GTATCTCATC AAGACCAGCA ATGCCCAGGC
    TGGGTCATCA TCGGACAGCC AGCAGCATCC CAGGTGTGTC GAACCAAGGT GATAGATCTC
    AGTGAAGGCA TTTCCCAGCA TGCCTGGTAC CCTTGCACCA TCAGCTACCC ATATTCCCAG
    CTGGCTCAGA CCACTTTTTG GCTCCGGGCG TATTTTTCTC AACCAATGGT TGCCGCAGCT
    GTCATTGTCC ACCTGGTGAC GGATGGGACA TATTATGGGG ACCAAAAGCA GGAGACCATC
    AGCGTGCAGC TGCTTGATAC CAAAGATCAG AGCCACGATC TAGGCCTCCA TGTCCTGAGC
    TGCAGGAACA ATCCCCTGAT TATCCCTGTG GTCCATGACC TCAGCCAGCC CTTCTACCAC
    AGCCAGGCGG TACGTGTGAG CTTCAGTTCG CCCCTGGTCG CCATCTCGGG GGTGGCCCTC
    CGTTCCTTCG ACAACTTTGA CCCCGTCACC CTGAGCAGCT GCCAGAGAGG GGAGACCTAC
    AGCCCTGCCG AGCAGAGCTG CGTGCACTTC GCATGTGAGA AAACTGACTG TCCAGAGCTG
    GCTGTGGAGA ATGCTTCTCT CAATTGCTCC AGCAGCGACC GCTACCACGG TGCCCAGTGT
    ACTGTGAGCT GCCGGACAGG CTACGTGCTC CAGATACGGC GGGATGATGA GCTGATCAAG
    AGCCAGACGG GACCCAGCGT CACAGTGACC TGTACAGAGG GCAAGTGGAA TAAGCAGGTG
    GCCTGTGAGC CAGTCGACTG CAGCATCCCA GATCACCATC AAGTCTATGC TGCCTCCTTC
    TCCTGCCCTG AGGGCACCAC CTTTGGCAGT CAATGTTCCT TCCAGTGCCG TCACCCTGCA
    CAATTGAAAG GCAACAACAG CCTCCTGACC TGCATGGAGG ATGGGCTGTG GTCCTTCCCA
    GAGGCCCTGT GTGAGCTCAT GTGCCTCGCT CCACCCCCTG TGCCCAATGC AGACCTCCAG
    ACCGCCCGGT GCCGAGAGAA TAAGCACAAG GTGGGCTCCT TCTGCAAATA CAAATGCAAG
    CCTGGATACC ATGTGCCTGG ATCCTCTCGG AAGTCAAAGA AACGGGCCTT CAAGACTCAG
    TGTACCCAGG ATGGCAGCTG GCAGGAGGGA GCTTGTGTTC CTGTGACCTG TGACCCACCT
    CCACCAAAAT TCCATGGGCT CTACCAGTGT ACTAATGGCT TCCAGTTCAA CAGTGAGTGT
    AGGATCAAGT GTGAAGACAG TGATGCCTCC CAGGGACTTG GGAGCAATGT CATTCATTGC
    CGGAAAGATG GCACCTGGAA CGGCTCCTTC CATGTCTGCC AGGAGATGCA AGGCCAGTGC
    TCGGTTCCAA ACGAGCTCAA CAGCAACCTC AAACTGCAGT GCCCTGATGG CTATGCCATA
    GGGTCGGAGT GTGCCACCTC GTGCCTGGAC CACAACAGCG AGTCCATCAT CCTGCCAATG
    AACGTGACCG TGCGTGACAT CCCCCACTGG CTGAACCCCA CACGGGTAGA GAGAGTTGTC
    TGCACTGCTG GTCTCAAGTG GTATCCTCAC CCTGCTCTGA TTCACTGTGT CAAAGGCTGT
    GAGCCCTTCA TGGGAGACAA TTATTGTGAT GCCATCAACA ACCGAGCCTT TTGCAACTAT
    GACGGTGGGG ATTGCTGCAC CTCCACAGTG AAGACCAAAA AGGTCACCCC ATTCCCTATG
    TCCTGTGATC TACAAGGTGA CTGTGCTTGT CGGGACCCCC AGGCCCAAGA ACACAGCCGG
    AAAGACCTCC GGGGATACAG CCATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43320
    Clone ID Related Accession (Same CDS sequence) XM_006717129.3 , XM_006717129.1 , XM_006717129.2
    Accession Version XM_006717129.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product pappalysin-1 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCATTT CTTTGAAAGA ATTGGGATCA GCATGTCATC TTTGCCTGGA AGGGAGAATC 
    CTGGTGCAGT ATGCTTCCAA CGCTTCCTCC CCAATGCCCT GCAGCCCATC AGGACACTGG
    AGCCCTCGTG AAGCAGAAGG TCATCCTGAT GTTGAACAGC CCTGTAAGTC CAGTGTCCGC
    ACCTGGAGCC CAAATTCAGC TGTCAACCCA CACACGGTTC CTCCAGCCTG CCCTGAGCCT
    CAAGGCTGCT ACCTCGAGCT GGAGTTCCTC TACCCCTTGG TCCCTGAGTC TCTGACCATT
    TGGGTGACCT TTGTCTCCAC TGACTGGGAC TCTAGTGGAG CTGTCAATGA CATCAAACTG
    TTGGCTGTCA GTGGGAAGAA CATCTCCCTG GGTCCTCAGA ATGTCTTCTG TGATGTCCCA
    CTGACCATCA GACTCTGGGA CGTGGGCGAG GAGGTGTATG GCATCCAAAT CTACACGCTG
    GATGAGCACC TGGAGATCGA TGCTGCCATG TTGACCTCCA CTGCAGACAC CCCACTCTGT
    CTACAGTGTA AGCCCCTGAA GTATAAGGTG GTCCGGGACC CTCCTCTCCA GATGGATGTG
    GCCTCCATCC TACATCTCAA TAGGAAATTC GTAGACATGG ATCTAAATCT TGGCAGTGTG
    TACCAGTATT GGGTCATAAC TATTTCAGGA ACTGAAGAGA GTGAGCCATC ACCTGCTGTC
    ACATACATCC ATGGAAGTGG GTACTGTGGC GATGGCATTA TACAAAAAGA CCAAGGTGAA
    CAATGCGACG ACATGAATAA GATCAATGGT GATGGCTGCT CCCTTTTCTG CCGACAAGAA
    GTCTCCTTCA ATTGTATTGA TGAACCCAGC CGGTGCTATT TCCATGATGG TGATGGGGTA
    TGTGAGGAGT TTGAACAAAA AACCAGCATT AAGGACTGTG GTGTCTACAC GCCCCAGGGA
    TTCCTGGATC AGTGGGCATC CAATGCTTCA GTATCTCATC AAGACCAGCA ATGCCCAGGC
    TGGGTCATCA TCGGACAGCC AGCAGCATCC CAGGTGTGTC GAACCAAGGT GATAGATCTC
    AGTGAAGGCA TTTCCCAGCA TGCCTGGTAC CCTTGCACCA TCAGCTACCC ATATTCCCAG
    CTGGCTCAGA CCACTTTTTG GCTCCGGGCG TATTTTTCTC AACCAATGGT TGCCGCAGCT
    GTCATTGTCC ACCTGGTGAC GGATGGGACA TATTATGGGG ACCAAAAGCA GGAGACCATC
    AGCGTGCAGC TGCTTGATAC CAAAGATCAG AGCCACGATC TAGGCCTCCA TGTCCTGAGC
    TGCAGGAACA ATCCCCTGAT TATCCCTGTG GTCCATGACC TCAGCCAGCC CTTCTACCAC
    AGCCAGGCGG TACGTGTGAG CTTCAGTTCG CCCCTGGTCG CCATCTCGGG GGTGGCCCTC
    CGTTCCTTCG ACAACTTTGA CCCCGTCACC CTGAGCAGCT GCCAGAGAGG GGAGACCTAC
    AGCCCTGCCG AGCAGAGCTG CGTGCACTTC GCATGTGAGA AAACTGACTG TCCAGAGCTG
    GCTGTGGAGA ATGCTTCTCT CAATTGCTCC AGCAGCGACC GCTACCACGG TGCCCAGTGT
    ACTGTGAGCT GCCGGACAGG CTACGTGCTC CAGATACGGC GGGATGATGA GCTGATCAAG
    AGCCAGACGG GACCCAGCGT CACAGTGACC TGTACAGAGG GCAAGTGGAA TAAGCAGGTG
    GCCTGTGAGC CAGTCGACTG CAGCATCCCA GATCACCATC AAGTCTATGC TGCCTCCTTC
    TCCTGCCCTG AGGGCACCAC CTTTGGCAGT CAATGTTCCT TCCAGTGCCG TCACCCTGCA
    CAATTGAAAG GCAACAACAG CCTCCTGACC TGCATGGAGG ATGGGCTGTG GTCCTTCCCA
    GAGGCCCTGT GTGAGCTCAT GTGCCTCGCT CCACCCCCTG TGCCCAATGC AGACCTCCAG
    ACCGCCCGGT GCCGAGAGAA TAAGCACAAG GTGGGCTCCT TCTGCAAATA CAAATGCAAG
    CCTGGATACC ATGTGCCTGG ATCCTCTCGG AAGTCAAAGA AACGGGCCTT CAAGACTCAG
    TGTACCCAGG ATGGCAGCTG GCAGGAGGGA GCTTGTGTTC CTGTGACCTG TGACCCACCT
    CCACCAAAAT TCCATGGGCT CTACCAGTGT ACTAATGGCT TCCAGTTCAA CAGTGAGTGT
    AGGATCAAGT GTGAAGACAG TGATGCCTCC CAGGGACTTG GGAGCAATGT CATTCATTGC
    CGGAAAGATG GCACCTGGAA CGGCTCCTTC CATGTCTGCC AGGAGATGCA AGGCCAGTGC
    TCGGTTCCAA ACGAGCTCAA CAGCAACCTC AAACTGCAGT GCCCTGATGG CTATGCCATA
    GGGTCGGAGT GTGCCACCTC GTGCCTGGAC CACAACAGCG AGTCCATCAT CCTGCCAATG
    AACGTGACCG TGCGTGACAT CCCCCACTGG CTGAACCCCA CACGGGTAGA GAGAGTTGTC
    TGCACTGCTG GTCTCAAGTG GTATCCTCAC CCTGCTCTGA TTCACTGTGT CAAAGGCTGT
    GAGCCCTTCA TGGGAGACAA TTATTGTGAT GCCATCAACA ACCGAGCCTT TTGCAACTAT
    GACGGTGGGG ATTGCTGCAC CTCCACAGTG AAGACCAAAA AGGTCACCCC ATTCCCTATG
    TCCTGTGATC TACAAGGTGA CTGTGCTTGT CGGGACCCCC AGGCCCAAGA ACACAGCCGG
    AAAGACCTCC GGGGATACAG CCATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006717192.1
    Translation

    Target ORF information:

    RefSeq Version XM_006717129.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens pregnancy-associated plasma protein A, pappalysin 1 (PAPPA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006717129.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCATTT CTTTGAAAGA ATTGGGATCA GCATGTCATC TTTGCCTGGA AGGGAGAATC 
    CTGGTGCAGT ATGCTTCCAA CGCTTCCTCC CCAATGCCCT GCAGCCCATC AGGACACTGG
    AGCCCTCGTG AAGCAGAAGG TCATCCTGAT GTTGAACAGC CCTGTAAGTC CAGTGTCCGC
    ACCTGGAGCC CAAATTCAGC TGTCAACCCA CACACGGTTC CTCCAGCCTG CCCTGAGCCT
    CAAGGCTGCT ACCTCGAGCT GGAGTTCCTC TACCCCTTGG TCCCTGAGTC TCTGACCATT
    TGGGTGACCT TTGTCTCCAC TGACTGGGAC TCTAGTGGAG CTGTCAATGA CATCAAACTG
    TTGGCTGTCA GTGGGAAGAA CATCTCCCTG GGTCCTCAGA ATGTCTTCTG TGATGTCCCA
    CTGACCATCA GACTCTGGGA CGTGGGCGAG GAGGTGTATG GCATCCAAAT CTACACGCTG
    GATGAGCACC TGGAGATCGA TGCTGCCATG TTGACCTCCA CTGCAGACAC CCCACTCTGT
    CTACAGTGTA AGCCCCTGAA GTATAAGGTG GTCCGGGACC CTCCTCTCCA GATGGATGTG
    GCCTCCATCC TACATCTCAA TAGGAAATTC GTAGACATGG ATCTAAATCT TGGCAGTGTG
    TACCAGTATT GGGTCATAAC TATTTCAGGA ACTGAAGAGA GTGAGCCATC ACCTGCTGTC
    ACATACATCC ATGGAAGTGG GTACTGTGGC GATGGCATTA TACAAAAAGA CCAAGGTGAA
    CAATGCGACG ACATGAATAA GATCAATGGT GATGGCTGCT CCCTTTTCTG CCGACAAGAA
    GTCTCCTTCA ATTGTATTGA TGAACCCAGC CGGTGCTATT TCCATGATGG TGATGGGGTA
    TGTGAGGAGT TTGAACAAAA AACCAGCATT AAGGACTGTG GTGTCTACAC GCCCCAGGGA
    TTCCTGGATC AGTGGGCATC CAATGCTTCA GTATCTCATC AAGACCAGCA ATGCCCAGGC
    TGGGTCATCA TCGGACAGCC AGCAGCATCC CAGGTGTGTC GAACCAAGGT GATAGATCTC
    AGTGAAGGCA TTTCCCAGCA TGCCTGGTAC CCTTGCACCA TCAGCTACCC ATATTCCCAG
    CTGGCTCAGA CCACTTTTTG GCTCCGGGCG TATTTTTCTC AACCAATGGT TGCCGCAGCT
    GTCATTGTCC ACCTGGTGAC GGATGGGACA TATTATGGGG ACCAAAAGCA GGAGACCATC
    AGCGTGCAGC TGCTTGATAC CAAAGATCAG AGCCACGATC TAGGCCTCCA TGTCCTGAGC
    TGCAGGAACA ATCCCCTGAT TATCCCTGTG GTCCATGACC TCAGCCAGCC CTTCTACCAC
    AGCCAGGCGG TACGTGTGAG CTTCAGTTCG CCCCTGGTCG CCATCTCGGG GGTGGCCCTC
    CGTTCCTTCG ACAACTTTGA CCCCGTCACC CTGAGCAGCT GCCAGAGAGG GGAGACCTAC
    AGCCCTGCCG AGCAGAGCTG CGTGCACTTC GCATGTGAGA AAACTGACTG TCCAGAGCTG
    GCTGTGGAGA ATGCTTCTCT CAATTGCTCC AGCAGCGACC GCTACCACGG TGCCCAGTGT
    ACTGTGAGCT GCCGGACAGG CTACGTGCTC CAGATACGGC GGGATGATGA GCTGATCAAG
    AGCCAGACGG GACCCAGCGT CACAGTGACC TGTACAGAGG GCAAGTGGAA TAAGCAGGTG
    GCCTGTGAGC CAGTCGACTG CAGCATCCCA GATCACCATC AAGTCTATGC TGCCTCCTTC
    TCCTGCCCTG AGGGCACCAC CTTTGGCAGT CAATGTTCCT TCCAGTGCCG TCACCCTGCA
    CAATTGAAAG GCAACAACAG CCTCCTGACC TGCATGGAGG ATGGGCTGTG GTCCTTCCCA
    GAGGCCCTGT GTGAGCTCAT GTGCCTCGCT CCACCCCCTG TGCCCAATGC AGACCTCCAG
    ACCGCCCGGT GCCGAGAGAA TAAGCACAAG GTGGGCTCCT TCTGCAAATA CAAATGCAAG
    CCTGGATACC ATGTGCCTGG ATCCTCTCGG AAGTCAAAGA AACGGGCCTT CAAGACTCAG
    TGTACCCAGG ATGGCAGCTG GCAGGAGGGA GCTTGTGTTC CTGTGACCTG TGACCCACCT
    CCACCAAAAT TCCATGGGCT CTACCAGTGT ACTAATGGCT TCCAGTTCAA CAGTGAGTGT
    AGGATCAAGT GTGAAGACAG TGATGCCTCC CAGGGACTTG GGAGCAATGT CATTCATTGC
    CGGAAAGATG GCACCTGGAA CGGCTCCTTC CATGTCTGCC AGGAGATGCA AGGCCAGTGC
    TCGGTTCCAA ACGAGCTCAA CAGCAACCTC AAACTGCAGT GCCCTGATGG CTATGCCATA
    GGGTCGGAGT GTGCCACCTC GTGCCTGGAC CACAACAGCG AGTCCATCAT CCTGCCAATG
    AACGTGACCG TGCGTGACAT CCCCCACTGG CTGAACCCCA CACGGGTAGA GAGAGTTGTC
    TGCACTGCTG GTCTCAAGTG GTATCCTCAC CCTGCTCTGA TTCACTGTGT CAAAGGCTGT
    GAGCCCTTCA TGGGAGACAA TTATTGTGAT GCCATCAACA ACCGAGCCTT TTGCAACTAT
    GACGGTGGGG ATTGCTGCAC CTCCACAGTG AAGACCAAAA AGGTCACCCC ATTCCCTATG
    TCCTGTGATC TACAAGGTGA CTGTGCTTGT CGGGACCCCC AGGCCCAAGA ACACAGCCGG
    AAAGACCTCC GGGGATACAG CCATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43320
    Clone ID Related Accession (Same CDS sequence) XM_006717129.3 , XM_006717129.1 , XM_006717129.2
    Accession Version XM_006717129.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2790bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product pappalysin-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006717129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCATTT CTTTGAAAGA ATTGGGATCA GCATGTCATC TTTGCCTGGA AGGGAGAATC 
    CTGGTGCAGT ATGCTTCCAA CGCTTCCTCC CCAATGCCCT GCAGCCCATC AGGACACTGG
    AGCCCTCGTG AAGCAGAAGG TCATCCTGAT GTTGAACAGC CCTGTAAGTC CAGTGTCCGC
    ACCTGGAGCC CAAATTCAGC TGTCAACCCA CACACGGTTC CTCCAGCCTG CCCTGAGCCT
    CAAGGCTGCT ACCTCGAGCT GGAGTTCCTC TACCCCTTGG TCCCTGAGTC TCTGACCATT
    TGGGTGACCT TTGTCTCCAC TGACTGGGAC TCTAGTGGAG CTGTCAATGA CATCAAACTG
    TTGGCTGTCA GTGGGAAGAA CATCTCCCTG GGTCCTCAGA ATGTCTTCTG TGATGTCCCA
    CTGACCATCA GACTCTGGGA CGTGGGCGAG GAGGTGTATG GCATCCAAAT CTACACGCTG
    GATGAGCACC TGGAGATCGA TGCTGCCATG TTGACCTCCA CTGCAGACAC CCCACTCTGT
    CTACAGTGTA AGCCCCTGAA GTATAAGGTG GTCCGGGACC CTCCTCTCCA GATGGATGTG
    GCCTCCATCC TACATCTCAA TAGGAAATTC GTAGACATGG ATCTAAATCT TGGCAGTGTG
    TACCAGTATT GGGTCATAAC TATTTCAGGA ACTGAAGAGA GTGAGCCATC ACCTGCTGTC
    ACATACATCC ATGGAAGTGG GTACTGTGGC GATGGCATTA TACAAAAAGA CCAAGGTGAA
    CAATGCGACG ACATGAATAA GATCAATGGT GATGGCTGCT CCCTTTTCTG CCGACAAGAA
    GTCTCCTTCA ATTGTATTGA TGAACCCAGC CGGTGCTATT TCCATGATGG TGATGGGGTA
    TGTGAGGAGT TTGAACAAAA AACCAGCATT AAGGACTGTG GTGTCTACAC GCCCCAGGGA
    TTCCTGGATC AGTGGGCATC CAATGCTTCA GTATCTCATC AAGACCAGCA ATGCCCAGGC
    TGGGTCATCA TCGGACAGCC AGCAGCATCC CAGGTGTGTC GAACCAAGGT GATAGATCTC
    AGTGAAGGCA TTTCCCAGCA TGCCTGGTAC CCTTGCACCA TCAGCTACCC ATATTCCCAG
    CTGGCTCAGA CCACTTTTTG GCTCCGGGCG TATTTTTCTC AACCAATGGT TGCCGCAGCT
    GTCATTGTCC ACCTGGTGAC GGATGGGACA TATTATGGGG ACCAAAAGCA GGAGACCATC
    AGCGTGCAGC TGCTTGATAC CAAAGATCAG AGCCACGATC TAGGCCTCCA TGTCCTGAGC
    TGCAGGAACA ATCCCCTGAT TATCCCTGTG GTCCATGACC TCAGCCAGCC CTTCTACCAC
    AGCCAGGCGG TACGTGTGAG CTTCAGTTCG CCCCTGGTCG CCATCTCGGG GGTGGCCCTC
    CGTTCCTTCG ACAACTTTGA CCCCGTCACC CTGAGCAGCT GCCAGAGAGG GGAGACCTAC
    AGCCCTGCCG AGCAGAGCTG CGTGCACTTC GCATGTGAGA AAACTGACTG TCCAGAGCTG
    GCTGTGGAGA ATGCTTCTCT CAATTGCTCC AGCAGCGACC GCTACCACGG TGCCCAGTGT
    ACTGTGAGCT GCCGGACAGG CTACGTGCTC CAGATACGGC GGGATGATGA GCTGATCAAG
    AGCCAGACGG GACCCAGCGT CACAGTGACC TGTACAGAGG GCAAGTGGAA TAAGCAGGTG
    GCCTGTGAGC CAGTCGACTG CAGCATCCCA GATCACCATC AAGTCTATGC TGCCTCCTTC
    TCCTGCCCTG AGGGCACCAC CTTTGGCAGT CAATGTTCCT TCCAGTGCCG TCACCCTGCA
    CAATTGAAAG GCAACAACAG CCTCCTGACC TGCATGGAGG ATGGGCTGTG GTCCTTCCCA
    GAGGCCCTGT GTGAGCTCAT GTGCCTCGCT CCACCCCCTG TGCCCAATGC AGACCTCCAG
    ACCGCCCGGT GCCGAGAGAA TAAGCACAAG GTGGGCTCCT TCTGCAAATA CAAATGCAAG
    CCTGGATACC ATGTGCCTGG ATCCTCTCGG AAGTCAAAGA AACGGGCCTT CAAGACTCAG
    TGTACCCAGG ATGGCAGCTG GCAGGAGGGA GCTTGTGTTC CTGTGACCTG TGACCCACCT
    CCACCAAAAT TCCATGGGCT CTACCAGTGT ACTAATGGCT TCCAGTTCAA CAGTGAGTGT
    AGGATCAAGT GTGAAGACAG TGATGCCTCC CAGGGACTTG GGAGCAATGT CATTCATTGC
    CGGAAAGATG GCACCTGGAA CGGCTCCTTC CATGTCTGCC AGGAGATGCA AGGCCAGTGC
    TCGGTTCCAA ACGAGCTCAA CAGCAACCTC AAACTGCAGT GCCCTGATGG CTATGCCATA
    GGGTCGGAGT GTGCCACCTC GTGCCTGGAC CACAACAGCG AGTCCATCAT CCTGCCAATG
    AACGTGACCG TGCGTGACAT CCCCCACTGG CTGAACCCCA CACGGGTAGA GAGAGTTGTC
    TGCACTGCTG GTCTCAAGTG GTATCCTCAC CCTGCTCTGA TTCACTGTGT CAAAGGCTGT
    GAGCCCTTCA TGGGAGACAA TTATTGTGAT GCCATCAACA ACCGAGCCTT TTGCAACTAT
    GACGGTGGGG ATTGCTGCAC CTCCACAGTG AAGACCAAAA AGGTCACCCC ATTCCCTATG
    TCCTGTGATC TACAAGGTGA CTGTGCTTGT CGGGACCCCC AGGCCCAAGA ACACAGCCGG
    AAAGACCTCC GGGGATACAG CCATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006717192.1
    CDS550..3339
    Translation

    Target ORF information:

    RefSeq Version XM_006717129.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pappalysin 1 (PAPPA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006717129.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCATTT CTTTGAAAGA ATTGGGATCA GCATGTCATC TTTGCCTGGA AGGGAGAATC 
    CTGGTGCAGT ATGCTTCCAA CGCTTCCTCC CCAATGCCCT GCAGCCCATC AGGACACTGG
    AGCCCTCGTG AAGCAGAAGG TCATCCTGAT GTTGAACAGC CCTGTAAGTC CAGTGTCCGC
    ACCTGGAGCC CAAATTCAGC TGTCAACCCA CACACGGTTC CTCCAGCCTG CCCTGAGCCT
    CAAGGCTGCT ACCTCGAGCT GGAGTTCCTC TACCCCTTGG TCCCTGAGTC TCTGACCATT
    TGGGTGACCT TTGTCTCCAC TGACTGGGAC TCTAGTGGAG CTGTCAATGA CATCAAACTG
    TTGGCTGTCA GTGGGAAGAA CATCTCCCTG GGTCCTCAGA ATGTCTTCTG TGATGTCCCA
    CTGACCATCA GACTCTGGGA CGTGGGCGAG GAGGTGTATG GCATCCAAAT CTACACGCTG
    GATGAGCACC TGGAGATCGA TGCTGCCATG TTGACCTCCA CTGCAGACAC CCCACTCTGT
    CTACAGTGTA AGCCCCTGAA GTATAAGGTG GTCCGGGACC CTCCTCTCCA GATGGATGTG
    GCCTCCATCC TACATCTCAA TAGGAAATTC GTAGACATGG ATCTAAATCT TGGCAGTGTG
    TACCAGTATT GGGTCATAAC TATTTCAGGA ACTGAAGAGA GTGAGCCATC ACCTGCTGTC
    ACATACATCC ATGGAAGTGG GTACTGTGGC GATGGCATTA TACAAAAAGA CCAAGGTGAA
    CAATGCGACG ACATGAATAA GATCAATGGT GATGGCTGCT CCCTTTTCTG CCGACAAGAA
    GTCTCCTTCA ATTGTATTGA TGAACCCAGC CGGTGCTATT TCCATGATGG TGATGGGGTA
    TGTGAGGAGT TTGAACAAAA AACCAGCATT AAGGACTGTG GTGTCTACAC GCCCCAGGGA
    TTCCTGGATC AGTGGGCATC CAATGCTTCA GTATCTCATC AAGACCAGCA ATGCCCAGGC
    TGGGTCATCA TCGGACAGCC AGCAGCATCC CAGGTGTGTC GAACCAAGGT GATAGATCTC
    AGTGAAGGCA TTTCCCAGCA TGCCTGGTAC CCTTGCACCA TCAGCTACCC ATATTCCCAG
    CTGGCTCAGA CCACTTTTTG GCTCCGGGCG TATTTTTCTC AACCAATGGT TGCCGCAGCT
    GTCATTGTCC ACCTGGTGAC GGATGGGACA TATTATGGGG ACCAAAAGCA GGAGACCATC
    AGCGTGCAGC TGCTTGATAC CAAAGATCAG AGCCACGATC TAGGCCTCCA TGTCCTGAGC
    TGCAGGAACA ATCCCCTGAT TATCCCTGTG GTCCATGACC TCAGCCAGCC CTTCTACCAC
    AGCCAGGCGG TACGTGTGAG CTTCAGTTCG CCCCTGGTCG CCATCTCGGG GGTGGCCCTC
    CGTTCCTTCG ACAACTTTGA CCCCGTCACC CTGAGCAGCT GCCAGAGAGG GGAGACCTAC
    AGCCCTGCCG AGCAGAGCTG CGTGCACTTC GCATGTGAGA AAACTGACTG TCCAGAGCTG
    GCTGTGGAGA ATGCTTCTCT CAATTGCTCC AGCAGCGACC GCTACCACGG TGCCCAGTGT
    ACTGTGAGCT GCCGGACAGG CTACGTGCTC CAGATACGGC GGGATGATGA GCTGATCAAG
    AGCCAGACGG GACCCAGCGT CACAGTGACC TGTACAGAGG GCAAGTGGAA TAAGCAGGTG
    GCCTGTGAGC CAGTCGACTG CAGCATCCCA GATCACCATC AAGTCTATGC TGCCTCCTTC
    TCCTGCCCTG AGGGCACCAC CTTTGGCAGT CAATGTTCCT TCCAGTGCCG TCACCCTGCA
    CAATTGAAAG GCAACAACAG CCTCCTGACC TGCATGGAGG ATGGGCTGTG GTCCTTCCCA
    GAGGCCCTGT GTGAGCTCAT GTGCCTCGCT CCACCCCCTG TGCCCAATGC AGACCTCCAG
    ACCGCCCGGT GCCGAGAGAA TAAGCACAAG GTGGGCTCCT TCTGCAAATA CAAATGCAAG
    CCTGGATACC ATGTGCCTGG ATCCTCTCGG AAGTCAAAGA AACGGGCCTT CAAGACTCAG
    TGTACCCAGG ATGGCAGCTG GCAGGAGGGA GCTTGTGTTC CTGTGACCTG TGACCCACCT
    CCACCAAAAT TCCATGGGCT CTACCAGTGT ACTAATGGCT TCCAGTTCAA CAGTGAGTGT
    AGGATCAAGT GTGAAGACAG TGATGCCTCC CAGGGACTTG GGAGCAATGT CATTCATTGC
    CGGAAAGATG GCACCTGGAA CGGCTCCTTC CATGTCTGCC AGGAGATGCA AGGCCAGTGC
    TCGGTTCCAA ACGAGCTCAA CAGCAACCTC AAACTGCAGT GCCCTGATGG CTATGCCATA
    GGGTCGGAGT GTGCCACCTC GTGCCTGGAC CACAACAGCG AGTCCATCAT CCTGCCAATG
    AACGTGACCG TGCGTGACAT CCCCCACTGG CTGAACCCCA CACGGGTAGA GAGAGTTGTC
    TGCACTGCTG GTCTCAAGTG GTATCCTCAC CCTGCTCTGA TTCACTGTGT CAAAGGCTGT
    GAGCCCTTCA TGGGAGACAA TTATTGTGAT GCCATCAACA ACCGAGCCTT TTGCAACTAT
    GACGGTGGGG ATTGCTGCAC CTCCACAGTG AAGACCAAAA AGGTCACCCC ATTCCCTATG
    TCCTGTGATC TACAAGGTGA CTGTGCTTGT CGGGACCCCC AGGCCCAAGA ACACAGCCGG
    AAAGACCTCC GGGGATACAG CCATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98680
    Clone ID Related Accession (Same CDS sequence) XM_017014784.1 , XM_017014784.2
    Accession Version XM_017014784.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4770bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product pappalysin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGCTCT GGAGTTGGGT GCTGCACCTG GGGCTGCTGA GCGCCGCGCT GGGCTGCGGG 
    CTGGCCGAGC GTCCCCGCCG GGCCCGGAGA GACCCGCGGG CCGGCCGACC CCCGCGCCCC
    GCCGCCGGCC CGGCCACCTG CGCCACCCGG GCGGCCCGCG GCCGCCGCGC CTCGCCGCCG
    CCGCCGCCGC CGCCGGGCGG TGCCTGGGAA GCCGTGCGCG TCCCCCGGCG GCGGCAGCAG
    CGGGAGGCGA GGGGCGCCAC CGAGGAGCCG AGCCCGCCGA GCCGGGCGCT CTATTTCAGC
    GGGCGAGGCG AGCAGCTGCG CCTCCGGGCC GACCTCGAGC TGCCCCGGGA CGCGTTCACG
    CTGCAAGTGT GGCTGCGAGC GGAGGGGGGC CAGAGGTCTC CGGCAGTGAT CACAGGGCTG
    TATGACAAAT GTTCTTATAT CTCACGTGAC CGAGGATGGG TCGTGGGCAT TCACACCATC
    AGTGACCAAG ACAACAAAGA CCCACGCTAC TTTTTCTCCT TGAAGACAGA CCGAGCCCGG
    CAAGTGACCA CCATCAATGC CCACCGCAGC TACCTCCCAG GCCAGTGGGT ATACCTAGCT
    GCCACCTATG ATGGGCAGTT CATGAAGCTC TATGTGAATG GTGCCCAGGT GGCCACCTCT
    GGGGAACAAG TGGGTGGCAT ATTCAGCCCA CTGACCCAGA AGTGCAAAGT GCTCATGTTA
    GGGGGCAGTG CCCTGAATCA CAACTACCGG GGCTACATCG AGCACTTCAG TCTGTGGAAG
    GTGGCCAGGA CTCAGCGGGA GATACTGTCT GACATGGAAA CCCATGGCGC CCACACTGCT
    CTACCTCAGC TCCTCCTCCA GGAGAACTGG GACAATGTGA AGCATGCCTG GTCCCCCATG
    AAGGATGGCA GCAGCCCCAA AGTGGAATTC AGCAATGCCC ACGGCTTTCT GCTGGACACG
    AGTCTGGAGC CTCCTCTGTG CGGACAGACA TTGTGTGACA ACACAGAGGT CATTGCCAGC
    TACAATCAGC TCTCAAGTTT CCGCCAGCCC AAGGTGGTGC GCTACCGCGT GGTCAACCTC
    TATGAAGATG ATCATAAGAA CCCGACGGTG ACGCGCGAGC AGGTGGACTT CCAGCACCAT
    CAGCTGGCTG AGGCCTTCAA GCAATACAAC ATCTCCTGGG AGCTGGACGT GCTGGAGGTG
    AGCAACTCCT CCCTTCGCCG CCGCCTCATC CTGGCCAACT GTGACATCAG CAAGATTGGG
    GATGAGAACT GTGACCCCGA GTGCAACCAC ACGCTGACGG GCCACGACGG CGGGGATTGC
    CGCCACCTGC GCCACCCTGC CTTCGTGAAG AAGCAGCACA ACGGGGTGTG TGACATGGAC
    TGCAACTATG AACGGTTCAA CTTTGATGGT GGAGAGTGCT GTGACCCTGA AATCACCAAT
    GTCACTCAGA CTTGCTTTGA CCCCGACTCT CCACACAGAG CCTACTTGGA TGTTAATGAG
    CTGAAGAACA TTCTTAAATT GGATGGATCA ACACATCTCA ATATTTTCTT TGCAAAATCC
    TCAGAGGAGG AGTTGGCAGG AGTAGCAACT TGGCCATGGG ACAAGGAGGC CCTGATGCAC
    TTAGGTGGCA TTGTCTTGAA CCCATCTTTC TATGGCATGC CTGGGCACAC CCACACCATG
    ATCCATGAGA TTGGTCACAG CCTGGGCCTC TATCACGTCT TCCGAGGCAT CTCAGAAATC
    CAGTCCTGCA GTGACCCCTG CATGGAGACA GAGCCCTCCT TCGAGACTGG AGACCTCTGC
    AATGATACCA ACCCAGCCCC TAAACACAAG TCCTGTGGTG ACCCAGGGCC AGGAAATGAC
    ACCTGTGGCT TTCATAGCTT CTTCAACACT CCTTACAACA ACTTCATGAG CTATGCAGAT
    GACGACTGTA CGGACTCCTT CACGCCCAAT CAAGTCGCCA GAATGCACTG TTACCTGGAC
    CTGGTCTACC AGGGCTGGCA GCCCTCCAGG AAACCAGCGC CTGTTGCCCT CGCCCCCCAA
    GTTCTGGGCC ACACAACGGA CTCTGTGACA CTGGAGTGGT TCCCACCTAT AGATGGCCAT
    TTCTTTGAAA GAGAATTGGG ATCAGCATGT CATCTTTGCC TGGAAGGGAG AATCCTGGTG
    CAGTATGCTT CCAACGCTTC CTCCCCAATG CCCTGCAGCC CATCAGGACA CTGGAGCCCT
    CGTGAAGCAG AAGGTCATCC TGATGTTGAA CAGCCCTGTA AGTCCAGTGT CCGCACCTGG
    AGCCCAAATT CAGCTGTCAA CCCACACACG GTTCCTCCAG CCTGCCCTGA GCCTCAAGGC
    TGCTACCTCG AGCTGGAGTT CCTCTACCCC TTGGTCCCTG AGTCTCTGAC CATTTGGGTG
    ACCTTTGTCT CCACTGACTG GGACTCTAGT GGAGCTGTCA ATGACATCAA ACTGTTGGCT
    GTCAGTGGGA AGAACATCTC CCTGGGTCCT CAGAATGTCT TCTGTGATGT CCCACTGACC
    ATCAGACTCT GGGACGTGGG CGAGGAGGTG TATGGCATCC AAATCTACAC GCTGGATGAG
    CACCTGGAGA TCGATGCTGC CATGTTGACC TCCACTGCAG ACACCCCACT CTGTCTACAG
    TGTAAGCCCC TGAAGTATAA GGTGGTCCGG GACCCTCCTC TCCAGATGGA TGTGGCCTCC
    ATCCTACATC TCAATAGGAA ATTCGTAGAC ATGGATCTAA ATCTTGGCAG TGTGTACCAG
    TATTGGGTCA TAACTATTTC AGGAACTGAA GAGAGTGAGC CATCACCTGC TGTCACATAC
    ATCCATGGAA GTGGGTACTG TGGCGATGGC ATTATACAAA AAGACCAAGG TGAACAATGC
    GACGACATGA ATAAGATCAA TGGTGATGGC TGCTCCCTTT TCTGCCGACA AGAAGTCTCC
    TTCAATTGTA TTGATGAACC CAGCCGGTGC TATTTCCATG ATGGTGATGG GGTATGTGAG
    GAGTTTGAAC AAAAAACCAG CATTAAGGAC TGTGGTGTCT ACACGCCCCA GGGATTCCTG
    GATCAGTGGG CATCCAATGC TTCAGTATCT CATCAAGACC AGCAATGCCC AGGCTGGGTC
    ATCATCGGAC AGCCAGCAGC ATCCCAGGCG TATTTTTCTC AACCAATGGT TGCCGCAGCT
    GTCATTGTCC ACCTGGTGAC GGATGGGACA TATTATGGGG ACCAAAAGCA GGAGACCATC
    AGCGTGCAGC TGCTTGATAC CAAAGATCAG AGCCACGATC TAGGCCTCCA TGTCCTGAGC
    TGCAGGAACA ATCCCCTGAT TATCCCTGTG GTCCATGACC TCAGCCAGCC CTTCTACCAC
    AGCCAGGCGG TACGTGTGAG CTTCAGTTCG CCCCTGGTCG CCATCTCGGG GGTGGCCCTC
    CGTTCCTTCG ACAACTTTGA CCCCGTCACC CTGAGCAGCT GCCAGAGAGG GGAGACCTAC
    AGCCCTGCCG AGCAGAGCTG CGTGCACTTC GCATGTGAGA AAACTGACTG TCCAGAGCTG
    GCTGTGGAGA ATGCTTCTCT CAATTGCTCC AGCAGCGACC GCTACCACGG TGCCCAGTGT
    ACTGTGAGCT GCCGGACAGG CTACGTGCTC CAGATACGGC GGGATGATGA GCTGATCAAG
    AGCCAGACGG GACCCAGCGT CACAGTGACC TGTACAGAGG GCAAGTGGAA TAAGCAGGTG
    GCCTGTGAGC CAGTCGACTG CAGCATCCCA GATCACCATC AAGTCTATGC TGCCTCCTTC
    TCCTGCCCTG AGGGCACCAC CTTTGGCAGT CAATGTTCCT TCCAGTGCCG TCACCCTGCA
    CAATTGAAAG GCAACAACAG CCTCCTGACC TGCATGGAGG ATGGGCTGTG GTCCTTCCCA
    GAGGCCCTGT GTGAGCTCAT GTGCCTCGCT CCACCCCCTG TGCCCAATGC AGACCTCCAG
    ACCGCCCGGT GCCGAGAGAA TAAGCACAAG GTGGGCTCCT TCTGCAAATA CAAATGCAAG
    CCTGGATACC ATGTGCCTGG ATCCTCTCGG AAGTCAAAGA AACGGGCCTT CAAGACTCAG
    TGTACCCAGG ATGGCAGCTG GCAGGAGGGA GCTTGTGTTC CTGTGACCTG TGACCCACCT
    CCACCAAAAT TCCATGGGCT CTACCAGTGT ACTAATGGCT TCCAGTTCAA CAGTGAGTGT
    AGGATCAAGT GTGAAGACAG TGATGCCTCC CAGGGACTTG GGAGCAATGT CATTCATTGC
    CGGAAAGATG GCACCTGGAA CGGCTCCTTC CATGTCTGCC AGGAGATGCA AGGCCAGTGC
    TCGGTTCCAA ACGAGCTCAA CAGCAACCTC AAACTGCAGT GCCCTGATGG CTATGCCATA
    GGGTCGGAGT GTGCCACCTC GTGCCTGGAC CACAACAGCG AGTCCATCAT CCTGCCAATG
    AACGTGACCG TGCGTGACAT CCCCCACTGG CTGAACCCCA CACGGGTAGA GAGAGTTGTC
    TGCACTGCTG GTCTCAAGTG GTATCCTCAC CCTGCTCTGA TTCACTGTGT CAAAGGCTGT
    GAGCCCTTCA TGGGAGACAA TTATTGTGAT GCCATCAACA ACCGAGCCTT TTGCAACTAT
    GACGGTGGGG ATTGCTGCAC CTCCACAGTG AAGACCAAAA AGGTCACCCC ATTCCCTATG
    TCCTGTGATC TACAAGGTGA CTGTGCTTGT CGGGACCCCC AGGCCCAAGA ACACAGCCGG
    AAAGACCTCC GGGGATACAG CCATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016870273.1
    CDS422..5191
    Translation

    Target ORF information:

    RefSeq Version XM_017014784.1
    Organism Homo sapiens(human)
    Definition Homo sapiens pappalysin 1 (PAPPA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGCTCT GGAGTTGGGT GCTGCACCTG GGGCTGCTGA GCGCCGCGCT GGGCTGCGGG 
    CTGGCCGAGC GTCCCCGCCG GGCCCGGAGA GACCCGCGGG CCGGCCGACC CCCGCGCCCC
    GCCGCCGGCC CGGCCACCTG CGCCACCCGG GCGGCCCGCG GCCGCCGCGC CTCGCCGCCG
    CCGCCGCCGC CGCCGGGCGG TGCCTGGGAA GCCGTGCGCG TCCCCCGGCG GCGGCAGCAG
    CGGGAGGCGA GGGGCGCCAC CGAGGAGCCG AGCCCGCCGA GCCGGGCGCT CTATTTCAGC
    GGGCGAGGCG AGCAGCTGCG CCTCCGGGCC GACCTCGAGC TGCCCCGGGA CGCGTTCACG
    CTGCAAGTGT GGCTGCGAGC GGAGGGGGGC CAGAGGTCTC CGGCAGTGAT CACAGGGCTG
    TATGACAAAT GTTCTTATAT CTCACGTGAC CGAGGATGGG TCGTGGGCAT TCACACCATC
    AGTGACCAAG ACAACAAAGA CCCACGCTAC TTTTTCTCCT TGAAGACAGA CCGAGCCCGG
    CAAGTGACCA CCATCAATGC CCACCGCAGC TACCTCCCAG GCCAGTGGGT ATACCTAGCT
    GCCACCTATG ATGGGCAGTT CATGAAGCTC TATGTGAATG GTGCCCAGGT GGCCACCTCT
    GGGGAACAAG TGGGTGGCAT ATTCAGCCCA CTGACCCAGA AGTGCAAAGT GCTCATGTTA
    GGGGGCAGTG CCCTGAATCA CAACTACCGG GGCTACATCG AGCACTTCAG TCTGTGGAAG
    GTGGCCAGGA CTCAGCGGGA GATACTGTCT GACATGGAAA CCCATGGCGC CCACACTGCT
    CTACCTCAGC TCCTCCTCCA GGAGAACTGG GACAATGTGA AGCATGCCTG GTCCCCCATG
    AAGGATGGCA GCAGCCCCAA AGTGGAATTC AGCAATGCCC ACGGCTTTCT GCTGGACACG
    AGTCTGGAGC CTCCTCTGTG CGGACAGACA TTGTGTGACA ACACAGAGGT CATTGCCAGC
    TACAATCAGC TCTCAAGTTT CCGCCAGCCC AAGGTGGTGC GCTACCGCGT GGTCAACCTC
    TATGAAGATG ATCATAAGAA CCCGACGGTG ACGCGCGAGC AGGTGGACTT CCAGCACCAT
    CAGCTGGCTG AGGCCTTCAA GCAATACAAC ATCTCCTGGG AGCTGGACGT GCTGGAGGTG
    AGCAACTCCT CCCTTCGCCG CCGCCTCATC CTGGCCAACT GTGACATCAG CAAGATTGGG
    GATGAGAACT GTGACCCCGA GTGCAACCAC ACGCTGACGG GCCACGACGG CGGGGATTGC
    CGCCACCTGC GCCACCCTGC CTTCGTGAAG AAGCAGCACA ACGGGGTGTG TGACATGGAC
    TGCAACTATG AACGGTTCAA CTTTGATGGT GGAGAGTGCT GTGACCCTGA AATCACCAAT
    GTCACTCAGA CTTGCTTTGA CCCCGACTCT CCACACAGAG CCTACTTGGA TGTTAATGAG
    CTGAAGAACA TTCTTAAATT GGATGGATCA ACACATCTCA ATATTTTCTT TGCAAAATCC
    TCAGAGGAGG AGTTGGCAGG AGTAGCAACT TGGCCATGGG ACAAGGAGGC CCTGATGCAC
    TTAGGTGGCA TTGTCTTGAA CCCATCTTTC TATGGCATGC CTGGGCACAC CCACACCATG
    ATCCATGAGA TTGGTCACAG CCTGGGCCTC TATCACGTCT TCCGAGGCAT CTCAGAAATC
    CAGTCCTGCA GTGACCCCTG CATGGAGACA GAGCCCTCCT TCGAGACTGG AGACCTCTGC
    AATGATACCA ACCCAGCCCC TAAACACAAG TCCTGTGGTG ACCCAGGGCC AGGAAATGAC
    ACCTGTGGCT TTCATAGCTT CTTCAACACT CCTTACAACA ACTTCATGAG CTATGCAGAT
    GACGACTGTA CGGACTCCTT CACGCCCAAT CAAGTCGCCA GAATGCACTG TTACCTGGAC
    CTGGTCTACC AGGGCTGGCA GCCCTCCAGG AAACCAGCGC CTGTTGCCCT CGCCCCCCAA
    GTTCTGGGCC ACACAACGGA CTCTGTGACA CTGGAGTGGT TCCCACCTAT AGATGGCCAT
    TTCTTTGAAA GAGAATTGGG ATCAGCATGT CATCTTTGCC TGGAAGGGAG AATCCTGGTG
    CAGTATGCTT CCAACGCTTC CTCCCCAATG CCCTGCAGCC CATCAGGACA CTGGAGCCCT
    CGTGAAGCAG AAGGTCATCC TGATGTTGAA CAGCCCTGTA AGTCCAGTGT CCGCACCTGG
    AGCCCAAATT CAGCTGTCAA CCCACACACG GTTCCTCCAG CCTGCCCTGA GCCTCAAGGC
    TGCTACCTCG AGCTGGAGTT CCTCTACCCC TTGGTCCCTG AGTCTCTGAC CATTTGGGTG
    ACCTTTGTCT CCACTGACTG GGACTCTAGT GGAGCTGTCA ATGACATCAA ACTGTTGGCT
    GTCAGTGGGA AGAACATCTC CCTGGGTCCT CAGAATGTCT TCTGTGATGT CCCACTGACC
    ATCAGACTCT GGGACGTGGG CGAGGAGGTG TATGGCATCC AAATCTACAC GCTGGATGAG
    CACCTGGAGA TCGATGCTGC CATGTTGACC TCCACTGCAG ACACCCCACT CTGTCTACAG
    TGTAAGCCCC TGAAGTATAA GGTGGTCCGG GACCCTCCTC TCCAGATGGA TGTGGCCTCC
    ATCCTACATC TCAATAGGAA ATTCGTAGAC ATGGATCTAA ATCTTGGCAG TGTGTACCAG
    TATTGGGTCA TAACTATTTC AGGAACTGAA GAGAGTGAGC CATCACCTGC TGTCACATAC
    ATCCATGGAA GTGGGTACTG TGGCGATGGC ATTATACAAA AAGACCAAGG TGAACAATGC
    GACGACATGA ATAAGATCAA TGGTGATGGC TGCTCCCTTT TCTGCCGACA AGAAGTCTCC
    TTCAATTGTA TTGATGAACC CAGCCGGTGC TATTTCCATG ATGGTGATGG GGTATGTGAG
    GAGTTTGAAC AAAAAACCAG CATTAAGGAC TGTGGTGTCT ACACGCCCCA GGGATTCCTG
    GATCAGTGGG CATCCAATGC TTCAGTATCT CATCAAGACC AGCAATGCCC AGGCTGGGTC
    ATCATCGGAC AGCCAGCAGC ATCCCAGGCG TATTTTTCTC AACCAATGGT TGCCGCAGCT
    GTCATTGTCC ACCTGGTGAC GGATGGGACA TATTATGGGG ACCAAAAGCA GGAGACCATC
    AGCGTGCAGC TGCTTGATAC CAAAGATCAG AGCCACGATC TAGGCCTCCA TGTCCTGAGC
    TGCAGGAACA ATCCCCTGAT TATCCCTGTG GTCCATGACC TCAGCCAGCC CTTCTACCAC
    AGCCAGGCGG TACGTGTGAG CTTCAGTTCG CCCCTGGTCG CCATCTCGGG GGTGGCCCTC
    CGTTCCTTCG ACAACTTTGA CCCCGTCACC CTGAGCAGCT GCCAGAGAGG GGAGACCTAC
    AGCCCTGCCG AGCAGAGCTG CGTGCACTTC GCATGTGAGA AAACTGACTG TCCAGAGCTG
    GCTGTGGAGA ATGCTTCTCT CAATTGCTCC AGCAGCGACC GCTACCACGG TGCCCAGTGT
    ACTGTGAGCT GCCGGACAGG CTACGTGCTC CAGATACGGC GGGATGATGA GCTGATCAAG
    AGCCAGACGG GACCCAGCGT CACAGTGACC TGTACAGAGG GCAAGTGGAA TAAGCAGGTG
    GCCTGTGAGC CAGTCGACTG CAGCATCCCA GATCACCATC AAGTCTATGC TGCCTCCTTC
    TCCTGCCCTG AGGGCACCAC CTTTGGCAGT CAATGTTCCT TCCAGTGCCG TCACCCTGCA
    CAATTGAAAG GCAACAACAG CCTCCTGACC TGCATGGAGG ATGGGCTGTG GTCCTTCCCA
    GAGGCCCTGT GTGAGCTCAT GTGCCTCGCT CCACCCCCTG TGCCCAATGC AGACCTCCAG
    ACCGCCCGGT GCCGAGAGAA TAAGCACAAG GTGGGCTCCT TCTGCAAATA CAAATGCAAG
    CCTGGATACC ATGTGCCTGG ATCCTCTCGG AAGTCAAAGA AACGGGCCTT CAAGACTCAG
    TGTACCCAGG ATGGCAGCTG GCAGGAGGGA GCTTGTGTTC CTGTGACCTG TGACCCACCT
    CCACCAAAAT TCCATGGGCT CTACCAGTGT ACTAATGGCT TCCAGTTCAA CAGTGAGTGT
    AGGATCAAGT GTGAAGACAG TGATGCCTCC CAGGGACTTG GGAGCAATGT CATTCATTGC
    CGGAAAGATG GCACCTGGAA CGGCTCCTTC CATGTCTGCC AGGAGATGCA AGGCCAGTGC
    TCGGTTCCAA ACGAGCTCAA CAGCAACCTC AAACTGCAGT GCCCTGATGG CTATGCCATA
    GGGTCGGAGT GTGCCACCTC GTGCCTGGAC CACAACAGCG AGTCCATCAT CCTGCCAATG
    AACGTGACCG TGCGTGACAT CCCCCACTGG CTGAACCCCA CACGGGTAGA GAGAGTTGTC
    TGCACTGCTG GTCTCAAGTG GTATCCTCAC CCTGCTCTGA TTCACTGTGT CAAAGGCTGT
    GAGCCCTTCA TGGGAGACAA TTATTGTGAT GCCATCAACA ACCGAGCCTT TTGCAACTAT
    GACGGTGGGG ATTGCTGCAC CTCCACAGTG AAGACCAAAA AGGTCACCCC ATTCCCTATG
    TCCTGTGATC TACAAGGTGA CTGTGCTTGT CGGGACCCCC AGGCCCAAGA ACACAGCCGG
    AAAGACCTCC GGGGATACAG CCATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu98680
    Clone ID Related Accession (Same CDS sequence) XM_017014784.1 , XM_017014784.2
    Accession Version XM_017014784.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4770bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product pappalysin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017014784.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGCTCT GGAGTTGGGT GCTGCACCTG GGGCTGCTGA GCGCCGCGCT GGGCTGCGGG 
    CTGGCCGAGC GTCCCCGCCG GGCCCGGAGA GACCCGCGGG CCGGCCGACC CCCGCGCCCC
    GCCGCCGGCC CGGCCACCTG CGCCACCCGG GCGGCCCGCG GCCGCCGCGC CTCGCCGCCG
    CCGCCGCCGC CGCCGGGCGG TGCCTGGGAA GCCGTGCGCG TCCCCCGGCG GCGGCAGCAG
    CGGGAGGCGA GGGGCGCCAC CGAGGAGCCG AGCCCGCCGA GCCGGGCGCT CTATTTCAGC
    GGGCGAGGCG AGCAGCTGCG CCTCCGGGCC GACCTCGAGC TGCCCCGGGA CGCGTTCACG
    CTGCAAGTGT GGCTGCGAGC GGAGGGGGGC CAGAGGTCTC CGGCAGTGAT CACAGGGCTG
    TATGACAAAT GTTCTTATAT CTCACGTGAC CGAGGATGGG TCGTGGGCAT TCACACCATC
    AGTGACCAAG ACAACAAAGA CCCACGCTAC TTTTTCTCCT TGAAGACAGA CCGAGCCCGG
    CAAGTGACCA CCATCAATGC CCACCGCAGC TACCTCCCAG GCCAGTGGGT ATACCTAGCT
    GCCACCTATG ATGGGCAGTT CATGAAGCTC TATGTGAATG GTGCCCAGGT GGCCACCTCT
    GGGGAACAAG TGGGTGGCAT ATTCAGCCCA CTGACCCAGA AGTGCAAAGT GCTCATGTTA
    GGGGGCAGTG CCCTGAATCA CAACTACCGG GGCTACATCG AGCACTTCAG TCTGTGGAAG
    GTGGCCAGGA CTCAGCGGGA GATACTGTCT GACATGGAAA CCCATGGCGC CCACACTGCT
    CTACCTCAGC TCCTCCTCCA GGAGAACTGG GACAATGTGA AGCATGCCTG GTCCCCCATG
    AAGGATGGCA GCAGCCCCAA AGTGGAATTC AGCAATGCCC ACGGCTTTCT GCTGGACACG
    AGTCTGGAGC CTCCTCTGTG CGGACAGACA TTGTGTGACA ACACAGAGGT CATTGCCAGC
    TACAATCAGC TCTCAAGTTT CCGCCAGCCC AAGGTGGTGC GCTACCGCGT GGTCAACCTC
    TATGAAGATG ATCATAAGAA CCCGACGGTG ACGCGCGAGC AGGTGGACTT CCAGCACCAT
    CAGCTGGCTG AGGCCTTCAA GCAATACAAC ATCTCCTGGG AGCTGGACGT GCTGGAGGTG
    AGCAACTCCT CCCTTCGCCG CCGCCTCATC CTGGCCAACT GTGACATCAG CAAGATTGGG
    GATGAGAACT GTGACCCCGA GTGCAACCAC ACGCTGACGG GCCACGACGG CGGGGATTGC
    CGCCACCTGC GCCACCCTGC CTTCGTGAAG AAGCAGCACA ACGGGGTGTG TGACATGGAC
    TGCAACTATG AACGGTTCAA CTTTGATGGT GGAGAGTGCT GTGACCCTGA AATCACCAAT
    GTCACTCAGA CTTGCTTTGA CCCCGACTCT CCACACAGAG CCTACTTGGA TGTTAATGAG
    CTGAAGAACA TTCTTAAATT GGATGGATCA ACACATCTCA ATATTTTCTT TGCAAAATCC
    TCAGAGGAGG AGTTGGCAGG AGTAGCAACT TGGCCATGGG ACAAGGAGGC CCTGATGCAC
    TTAGGTGGCA TTGTCTTGAA CCCATCTTTC TATGGCATGC CTGGGCACAC CCACACCATG
    ATCCATGAGA TTGGTCACAG CCTGGGCCTC TATCACGTCT TCCGAGGCAT CTCAGAAATC
    CAGTCCTGCA GTGACCCCTG CATGGAGACA GAGCCCTCCT TCGAGACTGG AGACCTCTGC
    AATGATACCA ACCCAGCCCC TAAACACAAG TCCTGTGGTG ACCCAGGGCC AGGAAATGAC
    ACCTGTGGCT TTCATAGCTT CTTCAACACT CCTTACAACA ACTTCATGAG CTATGCAGAT
    GACGACTGTA CGGACTCCTT CACGCCCAAT CAAGTCGCCA GAATGCACTG TTACCTGGAC
    CTGGTCTACC AGGGCTGGCA GCCCTCCAGG AAACCAGCGC CTGTTGCCCT CGCCCCCCAA
    GTTCTGGGCC ACACAACGGA CTCTGTGACA CTGGAGTGGT TCCCACCTAT AGATGGCCAT
    TTCTTTGAAA GAGAATTGGG ATCAGCATGT CATCTTTGCC TGGAAGGGAG AATCCTGGTG
    CAGTATGCTT CCAACGCTTC CTCCCCAATG CCCTGCAGCC CATCAGGACA CTGGAGCCCT
    CGTGAAGCAG AAGGTCATCC TGATGTTGAA CAGCCCTGTA AGTCCAGTGT CCGCACCTGG
    AGCCCAAATT CAGCTGTCAA CCCACACACG GTTCCTCCAG CCTGCCCTGA GCCTCAAGGC
    TGCTACCTCG AGCTGGAGTT CCTCTACCCC TTGGTCCCTG AGTCTCTGAC CATTTGGGTG
    ACCTTTGTCT CCACTGACTG GGACTCTAGT GGAGCTGTCA ATGACATCAA ACTGTTGGCT
    GTCAGTGGGA AGAACATCTC CCTGGGTCCT CAGAATGTCT TCTGTGATGT CCCACTGACC
    ATCAGACTCT GGGACGTGGG CGAGGAGGTG TATGGCATCC AAATCTACAC GCTGGATGAG
    CACCTGGAGA TCGATGCTGC CATGTTGACC TCCACTGCAG ACACCCCACT CTGTCTACAG
    TGTAAGCCCC TGAAGTATAA GGTGGTCCGG GACCCTCCTC TCCAGATGGA TGTGGCCTCC
    ATCCTACATC TCAATAGGAA ATTCGTAGAC ATGGATCTAA ATCTTGGCAG TGTGTACCAG
    TATTGGGTCA TAACTATTTC AGGAACTGAA GAGAGTGAGC CATCACCTGC TGTCACATAC
    ATCCATGGAA GTGGGTACTG TGGCGATGGC ATTATACAAA AAGACCAAGG TGAACAATGC
    GACGACATGA ATAAGATCAA TGGTGATGGC TGCTCCCTTT TCTGCCGACA AGAAGTCTCC
    TTCAATTGTA TTGATGAACC CAGCCGGTGC TATTTCCATG ATGGTGATGG GGTATGTGAG
    GAGTTTGAAC AAAAAACCAG CATTAAGGAC TGTGGTGTCT ACACGCCCCA GGGATTCCTG
    GATCAGTGGG CATCCAATGC TTCAGTATCT CATCAAGACC AGCAATGCCC AGGCTGGGTC
    ATCATCGGAC AGCCAGCAGC ATCCCAGGCG TATTTTTCTC AACCAATGGT TGCCGCAGCT
    GTCATTGTCC ACCTGGTGAC GGATGGGACA TATTATGGGG ACCAAAAGCA GGAGACCATC
    AGCGTGCAGC TGCTTGATAC CAAAGATCAG AGCCACGATC TAGGCCTCCA TGTCCTGAGC
    TGCAGGAACA ATCCCCTGAT TATCCCTGTG GTCCATGACC TCAGCCAGCC CTTCTACCAC
    AGCCAGGCGG TACGTGTGAG CTTCAGTTCG CCCCTGGTCG CCATCTCGGG GGTGGCCCTC
    CGTTCCTTCG ACAACTTTGA CCCCGTCACC CTGAGCAGCT GCCAGAGAGG GGAGACCTAC
    AGCCCTGCCG AGCAGAGCTG CGTGCACTTC GCATGTGAGA AAACTGACTG TCCAGAGCTG
    GCTGTGGAGA ATGCTTCTCT CAATTGCTCC AGCAGCGACC GCTACCACGG TGCCCAGTGT
    ACTGTGAGCT GCCGGACAGG CTACGTGCTC CAGATACGGC GGGATGATGA GCTGATCAAG
    AGCCAGACGG GACCCAGCGT CACAGTGACC TGTACAGAGG GCAAGTGGAA TAAGCAGGTG
    GCCTGTGAGC CAGTCGACTG CAGCATCCCA GATCACCATC AAGTCTATGC TGCCTCCTTC
    TCCTGCCCTG AGGGCACCAC CTTTGGCAGT CAATGTTCCT TCCAGTGCCG TCACCCTGCA
    CAATTGAAAG GCAACAACAG CCTCCTGACC TGCATGGAGG ATGGGCTGTG GTCCTTCCCA
    GAGGCCCTGT GTGAGCTCAT GTGCCTCGCT CCACCCCCTG TGCCCAATGC AGACCTCCAG
    ACCGCCCGGT GCCGAGAGAA TAAGCACAAG GTGGGCTCCT TCTGCAAATA CAAATGCAAG
    CCTGGATACC ATGTGCCTGG ATCCTCTCGG AAGTCAAAGA AACGGGCCTT CAAGACTCAG
    TGTACCCAGG ATGGCAGCTG GCAGGAGGGA GCTTGTGTTC CTGTGACCTG TGACCCACCT
    CCACCAAAAT TCCATGGGCT CTACCAGTGT ACTAATGGCT TCCAGTTCAA CAGTGAGTGT
    AGGATCAAGT GTGAAGACAG TGATGCCTCC CAGGGACTTG GGAGCAATGT CATTCATTGC
    CGGAAAGATG GCACCTGGAA CGGCTCCTTC CATGTCTGCC AGGAGATGCA AGGCCAGTGC
    TCGGTTCCAA ACGAGCTCAA CAGCAACCTC AAACTGCAGT GCCCTGATGG CTATGCCATA
    GGGTCGGAGT GTGCCACCTC GTGCCTGGAC CACAACAGCG AGTCCATCAT CCTGCCAATG
    AACGTGACCG TGCGTGACAT CCCCCACTGG CTGAACCCCA CACGGGTAGA GAGAGTTGTC
    TGCACTGCTG GTCTCAAGTG GTATCCTCAC CCTGCTCTGA TTCACTGTGT CAAAGGCTGT
    GAGCCCTTCA TGGGAGACAA TTATTGTGAT GCCATCAACA ACCGAGCCTT TTGCAACTAT
    GACGGTGGGG ATTGCTGCAC CTCCACAGTG AAGACCAAAA AGGTCACCCC ATTCCCTATG
    TCCTGTGATC TACAAGGTGA CTGTGCTTGT CGGGACCCCC AGGCCCAAGA ACACAGCCGG
    AAAGACCTCC GGGGATACAG CCATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016870273.1
    CDS422..5191
    Translation

    Target ORF information:

    RefSeq Version XM_017014784.2
    Organism Homo sapiens(human)
    Definition Homo sapiens pappalysin 1 (PAPPA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017014784.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCGGCTCT GGAGTTGGGT GCTGCACCTG GGGCTGCTGA GCGCCGCGCT GGGCTGCGGG 
    CTGGCCGAGC GTCCCCGCCG GGCCCGGAGA GACCCGCGGG CCGGCCGACC CCCGCGCCCC
    GCCGCCGGCC CGGCCACCTG CGCCACCCGG GCGGCCCGCG GCCGCCGCGC CTCGCCGCCG
    CCGCCGCCGC CGCCGGGCGG TGCCTGGGAA GCCGTGCGCG TCCCCCGGCG GCGGCAGCAG
    CGGGAGGCGA GGGGCGCCAC CGAGGAGCCG AGCCCGCCGA GCCGGGCGCT CTATTTCAGC
    GGGCGAGGCG AGCAGCTGCG CCTCCGGGCC GACCTCGAGC TGCCCCGGGA CGCGTTCACG
    CTGCAAGTGT GGCTGCGAGC GGAGGGGGGC CAGAGGTCTC CGGCAGTGAT CACAGGGCTG
    TATGACAAAT GTTCTTATAT CTCACGTGAC CGAGGATGGG TCGTGGGCAT TCACACCATC
    AGTGACCAAG ACAACAAAGA CCCACGCTAC TTTTTCTCCT TGAAGACAGA CCGAGCCCGG
    CAAGTGACCA CCATCAATGC CCACCGCAGC TACCTCCCAG GCCAGTGGGT ATACCTAGCT
    GCCACCTATG ATGGGCAGTT CATGAAGCTC TATGTGAATG GTGCCCAGGT GGCCACCTCT
    GGGGAACAAG TGGGTGGCAT ATTCAGCCCA CTGACCCAGA AGTGCAAAGT GCTCATGTTA
    GGGGGCAGTG CCCTGAATCA CAACTACCGG GGCTACATCG AGCACTTCAG TCTGTGGAAG
    GTGGCCAGGA CTCAGCGGGA GATACTGTCT GACATGGAAA CCCATGGCGC CCACACTGCT
    CTACCTCAGC TCCTCCTCCA GGAGAACTGG GACAATGTGA AGCATGCCTG GTCCCCCATG
    AAGGATGGCA GCAGCCCCAA AGTGGAATTC AGCAATGCCC ACGGCTTTCT GCTGGACACG
    AGTCTGGAGC CTCCTCTGTG CGGACAGACA TTGTGTGACA ACACAGAGGT CATTGCCAGC
    TACAATCAGC TCTCAAGTTT CCGCCAGCCC AAGGTGGTGC GCTACCGCGT GGTCAACCTC
    TATGAAGATG ATCATAAGAA CCCGACGGTG ACGCGCGAGC AGGTGGACTT CCAGCACCAT
    CAGCTGGCTG AGGCCTTCAA GCAATACAAC ATCTCCTGGG AGCTGGACGT GCTGGAGGTG
    AGCAACTCCT CCCTTCGCCG CCGCCTCATC CTGGCCAACT GTGACATCAG CAAGATTGGG
    GATGAGAACT GTGACCCCGA GTGCAACCAC ACGCTGACGG GCCACGACGG CGGGGATTGC
    CGCCACCTGC GCCACCCTGC CTTCGTGAAG AAGCAGCACA ACGGGGTGTG TGACATGGAC
    TGCAACTATG AACGGTTCAA CTTTGATGGT GGAGAGTGCT GTGACCCTGA AATCACCAAT
    GTCACTCAGA CTTGCTTTGA CCCCGACTCT CCACACAGAG CCTACTTGGA TGTTAATGAG
    CTGAAGAACA TTCTTAAATT GGATGGATCA ACACATCTCA ATATTTTCTT TGCAAAATCC
    TCAGAGGAGG AGTTGGCAGG AGTAGCAACT TGGCCATGGG ACAAGGAGGC CCTGATGCAC
    TTAGGTGGCA TTGTCTTGAA CCCATCTTTC TATGGCATGC CTGGGCACAC CCACACCATG
    ATCCATGAGA TTGGTCACAG CCTGGGCCTC TATCACGTCT TCCGAGGCAT CTCAGAAATC
    CAGTCCTGCA GTGACCCCTG CATGGAGACA GAGCCCTCCT TCGAGACTGG AGACCTCTGC
    AATGATACCA ACCCAGCCCC TAAACACAAG TCCTGTGGTG ACCCAGGGCC AGGAAATGAC
    ACCTGTGGCT TTCATAGCTT CTTCAACACT CCTTACAACA ACTTCATGAG CTATGCAGAT
    GACGACTGTA CGGACTCCTT CACGCCCAAT CAAGTCGCCA GAATGCACTG TTACCTGGAC
    CTGGTCTACC AGGGCTGGCA GCCCTCCAGG AAACCAGCGC CTGTTGCCCT CGCCCCCCAA
    GTTCTGGGCC ACACAACGGA CTCTGTGACA CTGGAGTGGT TCCCACCTAT AGATGGCCAT
    TTCTTTGAAA GAGAATTGGG ATCAGCATGT CATCTTTGCC TGGAAGGGAG AATCCTGGTG
    CAGTATGCTT CCAACGCTTC CTCCCCAATG CCCTGCAGCC CATCAGGACA CTGGAGCCCT
    CGTGAAGCAG AAGGTCATCC TGATGTTGAA CAGCCCTGTA AGTCCAGTGT CCGCACCTGG
    AGCCCAAATT CAGCTGTCAA CCCACACACG GTTCCTCCAG CCTGCCCTGA GCCTCAAGGC
    TGCTACCTCG AGCTGGAGTT CCTCTACCCC TTGGTCCCTG AGTCTCTGAC CATTTGGGTG
    ACCTTTGTCT CCACTGACTG GGACTCTAGT GGAGCTGTCA ATGACATCAA ACTGTTGGCT
    GTCAGTGGGA AGAACATCTC CCTGGGTCCT CAGAATGTCT TCTGTGATGT CCCACTGACC
    ATCAGACTCT GGGACGTGGG CGAGGAGGTG TATGGCATCC AAATCTACAC GCTGGATGAG
    CACCTGGAGA TCGATGCTGC CATGTTGACC TCCACTGCAG ACACCCCACT CTGTCTACAG
    TGTAAGCCCC TGAAGTATAA GGTGGTCCGG GACCCTCCTC TCCAGATGGA TGTGGCCTCC
    ATCCTACATC TCAATAGGAA ATTCGTAGAC ATGGATCTAA ATCTTGGCAG TGTGTACCAG
    TATTGGGTCA TAACTATTTC AGGAACTGAA GAGAGTGAGC CATCACCTGC TGTCACATAC
    ATCCATGGAA GTGGGTACTG TGGCGATGGC ATTATACAAA AAGACCAAGG TGAACAATGC
    GACGACATGA ATAAGATCAA TGGTGATGGC TGCTCCCTTT TCTGCCGACA AGAAGTCTCC
    TTCAATTGTA TTGATGAACC CAGCCGGTGC TATTTCCATG ATGGTGATGG GGTATGTGAG
    GAGTTTGAAC AAAAAACCAG CATTAAGGAC TGTGGTGTCT ACACGCCCCA GGGATTCCTG
    GATCAGTGGG CATCCAATGC TTCAGTATCT CATCAAGACC AGCAATGCCC AGGCTGGGTC
    ATCATCGGAC AGCCAGCAGC ATCCCAGGCG TATTTTTCTC AACCAATGGT TGCCGCAGCT
    GTCATTGTCC ACCTGGTGAC GGATGGGACA TATTATGGGG ACCAAAAGCA GGAGACCATC
    AGCGTGCAGC TGCTTGATAC CAAAGATCAG AGCCACGATC TAGGCCTCCA TGTCCTGAGC
    TGCAGGAACA ATCCCCTGAT TATCCCTGTG GTCCATGACC TCAGCCAGCC CTTCTACCAC
    AGCCAGGCGG TACGTGTGAG CTTCAGTTCG CCCCTGGTCG CCATCTCGGG GGTGGCCCTC
    CGTTCCTTCG ACAACTTTGA CCCCGTCACC CTGAGCAGCT GCCAGAGAGG GGAGACCTAC
    AGCCCTGCCG AGCAGAGCTG CGTGCACTTC GCATGTGAGA AAACTGACTG TCCAGAGCTG
    GCTGTGGAGA ATGCTTCTCT CAATTGCTCC AGCAGCGACC GCTACCACGG TGCCCAGTGT
    ACTGTGAGCT GCCGGACAGG CTACGTGCTC CAGATACGGC GGGATGATGA GCTGATCAAG
    AGCCAGACGG GACCCAGCGT CACAGTGACC TGTACAGAGG GCAAGTGGAA TAAGCAGGTG
    GCCTGTGAGC CAGTCGACTG CAGCATCCCA GATCACCATC AAGTCTATGC TGCCTCCTTC
    TCCTGCCCTG AGGGCACCAC CTTTGGCAGT CAATGTTCCT TCCAGTGCCG TCACCCTGCA
    CAATTGAAAG GCAACAACAG CCTCCTGACC TGCATGGAGG ATGGGCTGTG GTCCTTCCCA
    GAGGCCCTGT GTGAGCTCAT GTGCCTCGCT CCACCCCCTG TGCCCAATGC AGACCTCCAG
    ACCGCCCGGT GCCGAGAGAA TAAGCACAAG GTGGGCTCCT TCTGCAAATA CAAATGCAAG
    CCTGGATACC ATGTGCCTGG ATCCTCTCGG AAGTCAAAGA AACGGGCCTT CAAGACTCAG
    TGTACCCAGG ATGGCAGCTG GCAGGAGGGA GCTTGTGTTC CTGTGACCTG TGACCCACCT
    CCACCAAAAT TCCATGGGCT CTACCAGTGT ACTAATGGCT TCCAGTTCAA CAGTGAGTGT
    AGGATCAAGT GTGAAGACAG TGATGCCTCC CAGGGACTTG GGAGCAATGT CATTCATTGC
    CGGAAAGATG GCACCTGGAA CGGCTCCTTC CATGTCTGCC AGGAGATGCA AGGCCAGTGC
    TCGGTTCCAA ACGAGCTCAA CAGCAACCTC AAACTGCAGT GCCCTGATGG CTATGCCATA
    GGGTCGGAGT GTGCCACCTC GTGCCTGGAC CACAACAGCG AGTCCATCAT CCTGCCAATG
    AACGTGACCG TGCGTGACAT CCCCCACTGG CTGAACCCCA CACGGGTAGA GAGAGTTGTC
    TGCACTGCTG GTCTCAAGTG GTATCCTCAC CCTGCTCTGA TTCACTGTGT CAAAGGCTGT
    GAGCCCTTCA TGGGAGACAA TTATTGTGAT GCCATCAACA ACCGAGCCTT TTGCAACTAT
    GACGGTGGGG ATTGCTGCAC CTCCACAGTG AAGACCAAAA AGGTCACCCC ATTCCCTATG
    TCCTGTGATC TACAAGGTGA CTGTGCTTGT CGGGACCCCC AGGCCCAAGA ACACAGCCGG
    AAAGACCTCC GGGGATACAG CCATGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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