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GLRX5 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol GLRX5
Entrez Gene ID 51218
Full Name glutaredoxin 5
Synonyms C14orf87,FLB4739,GRX5,PR01238,PRO1238,PRSA,SIDBA3,SPAHGC
General protein information
Preferred Names

glutaredoxin 5

Names

glutaredoxin-related protein 5, mitochondrial
glutaredoxin 5 homolog
monothiol glutaredoxin-5

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

14

14q32.13

Summary This gene encodes a mitochondrial protein, which is evolutionarily conserved. It is involved in the biogenesis of iron-sulfur clusters, which are required for normal iron homeostasis. Mutations in this gene are associated with autosomal recessive pyridoxine-refractory sideroblastic anemia. [provided by RefSeq, May 2010].
Disorder MIM:

609588

Disorder Html: Anemia, sideroblastic, pyridoxine-refractory, autosomal recessive,

mRNA and Protein(s)

mRNA Protein Name
NM_016417.2 NP_057501.2 glutaredoxin-related protein 5, mitochondrial precursor



Gallus gallus (chicken) GLRX5 NP_001008472.1
Danio rerio (zebrafish) glrx5 NP_998186.1
Kluyveromyces lactis KLLA0B09636g XP_451957.1
Rattus norvegicus (Norway rat) Glrx5 NP_001102192.1
Drosophila melanogaster (fruit fly) CG14407 NP_572974.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP002500 XP_312440.3
Bos taurus (cattle) GLRX5 NP_001093773.1
Caenorhabditis elegans (roundworm) glrx-5 NP_499610.1
Saccharomyces cerevisiae (baker's yeast) GRX5 NP_015266.1
Schizosaccharomyces pombe (fission yeast) grx5 NP_593888.1
Macaca mulatta (rhesus monkey) GLRX5 XP_001101070.1
Homo sapiens (human) GLRX5 NP_057501.2
Pan troglodytes (chimpanzee) GLRX5 XP_001154482.1
Mus musculus (house mouse) Glrx5 NP_082695.1
Eremothecium gossypii AGOS_ADR053W NP_984149.1
Magnaporthe oryzae (rice blast fungus) MGG_09542 XP_003712199.1
Neurospora crassa NCU04098 XP_960860.1
Xenopus tropicalis (tropical clawed frog) glrx5 NP_001004919.1


Related articles in PubMed

Genome-wide association study combining pathway analysis for typical sporadic amyotrophic lateral sclerosis in Chinese Han populations.
Xie T, Deng L, Mei P, Zhou Y, Wang B, Zhang J, Lin J, Wei Y, Zhang X, Xu R
Neurobiology of aging35(7)1778.e9-1778.e23(2014 Jul)

Variant non ketotic hyperglycinemia is caused by mutations in LIAS, BOLA3 and the novel gene GLRX5.
Baker PR 2nd, Friederich MW, Swanson MA, Shaikh T, Bhattacharya K, Scharer GH, Aicher J, Creadon-Swindell G, Geiger E, MacLean KN, Lee WT, Deshpande C, Freckmann ML, Shih LY, Wasserstein M, Rasmussen MB, Lund AM, Procopis P, Cameron JM, Robinson BH, Brown GK, Brown RM, Compton AG, Dieckmann CL, Collard R, Coughlin CR 2nd, Spector E, Wempe MF, Van Hove JL
Brain : a journal of neurology137(Pt 2)366-79(2014 Feb)

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA, Butte NF
PloS one7(12)e51954(2012)

The crystal structure of human GLRX5: iron-sulfur cluster co-ordination, tetrameric assembly and monomer activity.
Johansson C, Roos AK, Montano SJ, Sengupta R, Filippakopoulos P, Guo K, von Delft F, Holmgren A, Oppermann U, Kavanagh KL
The Biochemical journal433(2)303-11(2011 Jan)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Patients with GLRX5-associated variant nonketotic hyperglycemia had normal development with childhood-onset spastic paraplegia, spinal lesion, and optic atrophy.
Title: Variant non ketotic hyperglycinemia is caused by mutations in LIAS, BOLA3 and the novel gene GLRX5.

crystal structure of GLRX5 revealed a tetrameric organization with the [2Fe-2S] clusters buried in the interior and shielded from the solvent by the conserved beta1-alpha2 loop
Title: The crystal structure of human GLRX5: iron-sulfur cluster co-ordination, tetrameric assembly and monomer activity.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Glutaredoxin 5 deficiency causes sideroblastic anemia by specifically impairing heme biosynthesis and depleting cytosolic iron in human erythroblasts
Title: Glutaredoxin 5 deficiency causes sideroblastic anemia by specifically impairing heme biosynthesis and depleting cytosolic iron in human erythroblasts.

The following GLRX5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLRX5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu18481 NM_016417.2
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Homo sapiens glutaredoxin 5 (GLRX5), mRNA. pcDNA3.1+/C-(K)DYK or customized vector
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu18481
Clone ID Related Accession (Same CDS sequence) NM_016417.2
Accession Version NM_016417.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 474bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-26
Organism Homo sapiens(Human)
Product glutaredoxin-related protein 5, mitochondrial precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI259789.1, BX248075.1, BC047680.1, BI834047.1 and AI089763.1. This sequence is a reference standard in the RefSeqGene project. On Feb 10, 2004 this sequence version replaced NM_016417.1. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.2408.1, ERR279834.923.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
ATGAGCGGGT CCCTCGGCCG AGCTGCGGCG GCTCTGCTCC GCTGGGGGCG CGGCGCGGGC 
GGCGGTGGCC TTTGGGGTCC GGGCGTGCGG GCGGCGGGCT CGGGCGCGGG CGGCGGCGGC
TCGGCGGAGC AGTTGGACGC GCTGGTGAAG AAGGACAAGG TGGTGGTCTT CCTCAAGGGG
ACGCCGGAGC AGCCCCAGTG CGGCTTCAGC AACGCCGTGG TGCAGATCCT GCGGCTGCAC
GGCGTCCGCG ATTACGCGGC CTACAACGTG CTGGACGACC CGGAGCTCCG ACAAGGCATT
AAAGACTATT CCAACTGGCC CACCATCCCG CAAGTGTACC TCAATGGCGA GTTTGTAGGG
GGCTGTGACA TTCTTCTGCA GATGCACCAG AATGGGGACT TGGTGGAAGA ACTGAAAAAG
CTGGGGATCC ACTCCGCCCT TTTAGATGAA AAGAAAGACC AAGACTCCAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_057501.2
CDS105..578
Misc Feature(1)3..5
Misc Feature(2)237..509
Misc Feature(3)order(279..281,303..311,426..431)
Misc Feature(4)279..281
Misc Feature(5)order(303..305,312..314)
Misc Feature(6)393..407
Misc Feature(7)468..473
Misc Feature(8)570..572
Exon (1)1..399
Exon (2)400..1171
Translation

Target ORF information:

RefSeq Version NM_016417.2
Organism Homo sapiens(Human)
Definition Homo sapiens glutaredoxin 5 (GLRX5), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_016417.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
ATGAGCGGGT CCCTCGGCCG AGCTGCGGCG GCTCTGCTCC GCTGGGGGCG CGGCGCGGGC 
GGCGGTGGCC TTTGGGGTCC GGGCGTGCGG GCGGCGGGCT CGGGCGCGGG CGGCGGCGGC
TCGGCGGAGC AGTTGGACGC GCTGGTGAAG AAGGACAAGG TGGTGGTCTT CCTCAAGGGG
ACGCCGGAGC AGCCCCAGTG CGGCTTCAGC AACGCCGTGG TGCAGATCCT GCGGCTGCAC
GGCGTCCGCG ATTACGCGGC CTACAACGTG CTGGACGACC CGGAGCTCCG ACAAGGCATT
AAAGACTATT CCAACTGGCC CACCATCCCG CAAGTGTACC TCAATGGCGA GTTTGTAGGG
GGCTGTGACA TTCTTCTGCA GATGCACCAG AATGGGGACT TGGTGGAAGA ACTGAAAAAG
CTGGGGATCC ACTCCGCCCT TTTAGATGAA AAGAAAGACC AAGACTCCAA GTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Genome-wide association study combining pathway analysis for typical sporadic amyotrophic lateral sclerosis in Chinese Han populations.
Neurobiology of aging35(7)1778.e9-1778.e23(2014 Jul)
Xie T,Deng L,Mei P,Zhou Y,Wang B,Zhang J,Lin J,Wei Y,Zhang X,Xu R


book

Variant non ketotic hyperglycinemia is caused by mutations in LIAS, BOLA3 and the novel gene GLRX5.
Brain : a journal of neurology137(Pt 2)366-79(2014 Feb)
Baker PR 2nd,Friederich MW,Swanson MA,Shaikh T,Bhattacharya K,Scharer GH,Aicher J,Creadon-Swindell G,Geiger E,MacLean KN,Lee WT,Deshpande C,Freckmann ML,Shih LY,Wasserstein M,Rasmussen MB,Lund AM,Procopis P,Cameron JM,Robinson BH,Brown GK,Brown RM,Compton AG,Dieckmann CL,Collard R,Coughlin CR 2nd,Spector E,Wempe MF,Van Hove JL


book

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
PloS one7(12)e51954(2012)
Comuzzie AG,Cole SA,Laston SL,Voruganti VS,Haack K,Gibbs RA,Butte NF


book

The crystal structure of human GLRX5: iron-sulfur cluster co-ordination, tetrameric assembly and monomer activity.
The Biochemical journal433(2)303-11(2011 Jan)
Johansson C,Roos AK,Montano SJ,Sengupta R,Filippakopoulos P,Guo K,von Delft F,Holmgren A,Oppermann U,Kavanagh KL


book

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ


 
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